CN107789619A - A kind of alimentary canal mucus cleaning agent - Google Patents
A kind of alimentary canal mucus cleaning agent Download PDFInfo
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- CN107789619A CN107789619A CN201610766046.3A CN201610766046A CN107789619A CN 107789619 A CN107789619 A CN 107789619A CN 201610766046 A CN201610766046 A CN 201610766046A CN 107789619 A CN107789619 A CN 107789619A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
- A61K38/482—Serine endopeptidases (3.4.21)
- A61K38/4826—Trypsin (3.4.21.4) Chymotrypsin (3.4.21.1)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/02—Inorganic compounds
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- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
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- A—HUMAN NECESSITIES
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- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/24—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing atoms other than carbon, hydrogen, oxygen, halogen, nitrogen or sulfur, e.g. cyclomethicone or phospholipids
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- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21004—Trypsin (3.4.21.4)
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Abstract
It is an object of the invention to provide a kind of alimentary canal mucus cleaning agent, is particluarly suitable for neutral or hydrolyzed under basic conditions alimentary canal mucus.Its composition includes neutral proteinase, trypsase and excipient, and the invention also discloses the preparation method of alimentary canal mucus cleaning agent.The beneficial effects of the invention are as follows the stability that can improve protease to greatest extent, alimentary canal mucus cleaning agent is stored under normal temperature condition, without cryogenic storage facilities such as refrigerators, be very easy to practicality, reduce use cost.Preparation technology is simple, reduces production cost, can effectively decompose the mucus in alimentary canal, reduces the generation because mucus foam in alimentary canal, can simplify endoscope diagnosis and treatment operating process, improves the definition in the visual field during endoscope diagnosis and treatment.
Description
Technical field
The invention belongs to pharmaceutical technology field, more particularly, to a kind of alimentary canal mucus cleaning agent, for digestive endoscope
Check and adjuvant drug before treatment or imageological examination.
Background technology
Digestive endoscopy refers to that in the case where light illuminates intestines and stomach and a kind of tubulose of abdominal visceral browser lesion can be looked at straight
Instrumental examination, it can both make to diagnose to confirm to the end through what other inspection methods obtained, and can also make to be not easy to make a definite diagnosis through other method
Early lesion made a definite diagnosis, particularly to early gastrointestinal carcinoma swell discovery play the role of uniqueness.But during diagnosis,
Due to a large amount of foams and mucus in alimentary canal be present, cause the visual field unclear, review time length, increase patient suffering, and easily
Cause mistaken diagnosis and fail to pinpoint a disease in diagnosis, be also unfavorable for the early diagnosis and discovery of disease of digestive tract.
The alimentary canal mucus cleaning agent of prior art uses pepsin, trypsase or chain protease by mucus water water more
Solution, and dimeticone is added as defoamer, to eliminate the mucus and foam in digestive system.Pepsin (English name：
Pepsin it is) a kind of digestible protein enzyme, work(secreted by the gastric chief cells (gastric chief cell) in stomach
It by the breaks down proteins in food is small fragments of peptides that can be.Trypsase Trypsin (Parenzyme) is the one of protease
Kind, it is from a kind of serine protein hydrolase of the pancreas extraction of ox, sheep, pig, is only broken lysine or arginine participates in being formed
Peptide bond, pH be 8.0, temperature be 37 DEG C when, the ability to function of trypsin solution is most strong.Chain protease is from streptococcus
The mixture of a kind of serinase and acid protease that are separated in (Streptomyces griseus).
Pepsin has greater activity in sour environment, and its optimum pH is about 3.Neutral or alkaline ph values molten
In liquid, pepsin can unwind and loss of activity.Digestive endoscopy typically enters under 6 hour conditions above on an empty stomach
OK, stomach pH value in emptying about between 7.0~7.2, is grown using pepsin as alimentary canal mucus cleaning agent scavenging period,
Effect is poor.
Alimentary canal mucus composition is the main mucoprotein to be made up of mucopolysaccharide, and its amino and carboxy-terminal end region are slight
Glycosylation, and it is rich in cysteine.Cysteine residues participate in establishing the contact in two sulphur between mucoprotein monomer.Mucoprotein
10~80 residue sequences multiple tandem repetitive sequences, wherein the amino acid of the up to big middle section of the formation of half is
Serine or threonine.It is also undesirable using trypsin hydrolysis alimentary canal mucus, hydrolysis time length, effect.
Chain protease optimal pH is similarly acid, and hydrolysis effect is poor.
Number of patent application：201010136337.7 disclosing one kind is used for gastral pharmaceutical composition and its application, institute
Stating composition includes the first pharmacological activity component A containing at least one digestive ferment and containing at least one silicone oil analog
Second pharmacological activity component B.Said composition can be made into various pharmaceutically acceptable formulations；Composition is being prepared as digestion
Endoscopy has defoaming effect with treatment or the application of the preoperative adjuvant drug given of imageological examination, said composition, simultaneously
Also have and dispel mucus effect, digestive endoscopy inspection and the degree of getting a clear view for the treatment of or imageological examination can be improved, reduce mistaken diagnosis
With fail to pinpoint a disease in diagnosis, improve the validity of diagnosis and treatment, beneficial to disease of digestive tract early diagnosis find.
The alimentary canal mucus cleaning agent of prior art needs freezen protective, and the holding time is short, and hydrolysis effect is poor.
The content of the invention
It is an object of the invention to provide a kind of stability is high, can normal temperature storage, the alimentary canal mucus cleaning of holding time length
Agent
The technical scheme is that：
A kind of alimentary canal mucus cleaning agent of the present invention, including neutral proteinase, trypsase and excipient, neutral proteinase
Optimal pH be 7.5-10.5, the neutral proteinase is fermentation of bacillus subtilis generation.
The present invention hydrolyzes alimentary canal mucus using neutral proteinase and trypsase, can be directed to the spy of alimentary canal mucus
Point, many peptide bonds containing serine are targetedly hydrolyzed under weak basic condition, hydrolysis time is short, and effect is good.Can be at 5 minutes
Within the foam in alimentary canal is hydrolyzed it is complete.
The excipient includes pH adjusting agent, and the pH adjusting agent is one kind in carbonate, phosphate or borate
It is or several.
In order to further safeguard the weakly alkaline environment in alimentary canal, pH adjusting agent can be added in excipient.
Also include stabilizer in the excipient, the stabilizer is in 4- formylphenyl boronic acids, calcium chloride or sorbierite
One or more.
Boric acid is proteinase activity inhibitor, 4- formylphenyl boronic acids can protease inhibition activity, protease inhibition pair
The degraded of itself, the stability of protease is improved, the calcium ion of alimentary canal mucus cleaning agent of the invention can be with protease
Activated centre combines, and makes the active three-dimensional structure spatial stability of protease, is firmly combined together the peptide chain of protease, increases
The stability of strong protease, while calcium ion can also neutralize the negative electrical charge on protease surface, eliminate charged group to protease
Adverse effect.Sorbierite in stabilizer is combined with the micro water of protein surface, lowers hydrone to proteinase activity
Influence, improve protease stability.
The stabilizer is calcium chloride and sorbierite.
The alimentary canal mucus cleaning agent of the present invention, by the way that calcium chloride and sorbierite are used in combination, can be carried to greatest extent
The stability of high protein enzyme, alimentary canal mucus cleaning agent is stored under normal temperature condition, set without low-temperature storages such as refrigerators
It is standby, practicality is very easy to, reduces use cost.
The alimentary canal mucus cleaning agent includes neutral proteinase 5-30 parts by weight, trypsase 5-30 parts by weight and figuration
Agent 10-130 parts by weight.
The excipient includes pH adjusting agent 5-55 parts by weight, defoamer 0.2-5.0 parts by weight, stabilizer 4.8-60 weight
Part, antioxidant 0-5 parts by weight, and flavouring 0-5 parts by weight.
The stabilizer includes calcium chloride 0.001-2 parts by weight and sorbierite 4.799-58 parts by weight.
Researcher of the present invention passes through long-term lot of experiments, and discovery uses calcium chloride 0.001-2 parts by weight and sorbierite
The stabilizer of 4.799-58 parts by weight, the activity of 12 months enzymes of preservation at normal temperatures of alimentary canal mucus cleaning agent are maintained at
More than 90%.
The granularity of the neutral proteinase is 40-200 mesh, and preferably the granularity of neutral proteinase is 80-160 mesh.
A kind of preparation method of described alimentary canal mucus cleaning agent, comprises the following steps：
(1) load weighted neutral proteinase and excipient are crossed into 80 mesh sieves, mixes 15-30min afterwards；
(2) mass concentration for adding neutral proteinase and excipient gross weight 5-15% parts by weight is molten for 50% alcohol
Liquid, continue to mix 20-50min；
(3) neutral proteinase mixed and excipient are dried into 20-60mim under the conditions of 30-65 DEG C, it is clear obtains mucous membrane
Lotion.
The preparation method technique is simple, and protease need not use the modes such as parcel to carry out physical separation with unclassified stores,
Protease in the alimentary canal mucus cleaning agent of the present invention is directly mixed with unclassified stores, can simplify production technology
And flow, reduce production cost.
It is mixed into and is stirred in the step (1), stir speed (S.S.) is that 40-120 turns/min.
The alimentary canal mucus cleaning agent of the present invention, protease can effectively decompose the mucus in alimentary canal, reduce because viscous
The generation of liquid foam in alimentary canal, therefore avoid the need for using flushing of traditional defoamer to mucus spume in alimentary canal,
Endoscope diagnosis and treatment operating process can be simplified, improve the definition in the visual field during endoscope diagnosis and treatment.
Embodiment
Embodiment 1
The parts by weight of neutral proteinase 5.0, the parts by weight of trypsase 5.0, the parts by weight of Tween-80 0.17, Arlacel-80 0.13
The parts by weight of 0.18 parts by weight of silica of parts by weight dimethicone 0.001, the parts by weight of sodium acid carbonate 0.69, sodium dihydrogen phosphate
4.31 parts by weight, the parts by weight of calcium chloride 0.001, the parts by weight of sorbierite 4.799.
80 mesh sieves are crossed after above-mentioned medicine is weighed, mix 30min, the mass concentration for adding 5 parts by weight is 50% alcohol
Solution, 50min is stirred under conditions of 40 turns/min, by the neutral proteinase mixed and excipient under the conditions of 65 DEG C
20mim is dried, obtains mucous membrane cleaning agent.
Embodiment 2
The parts by weight of neutral proteinase 5.0, the parts by weight of trypsase 5.0, the parts by weight of Tween-80 1.21, Arlacel-60 0.58
The parts by weight of 0.21 parts by weight of silica of parts by weight dimethicone 0.13, the parts by weight of sodium carbonate 0.58, sodium dihydrogen phosphate
7.26 parts by weight, the parts by weight of calcium chloride 0.005, the parts by weight of sorbierite 12.34.
80 mesh sieves are crossed after above-mentioned medicine is weighed, mix 15min, the mass concentration for adding 10 parts by weight is 50% alcohol
Solution, 30min is stirred under conditions of 80 turns/min, by the neutral proteinase mixed and excipient under the conditions of 30 DEG C
60mim is dried, obtains mucous membrane cleaning agent.
Embodiment 3
The parts by weight of neutral proteinase 10.0, the parts by weight of trypsase 10.0, the parts by weight of Tween-80 0.97, Arlacel-80
The parts by weight of 0.63 parts by weight dimethicone, 0.38 parts by weight of silica 0.002, the parts by weight of disodium hydrogen phosphate 15.69, phosphorus
The parts by weight of acid dihydride sodium 4.31, the parts by weight of calcium chloride 0.001, the parts by weight of sorbierite 9.05.
80 mesh sieves are crossed after above-mentioned medicine is weighed, mix 30min, the mass concentration for adding 13 parts by weight is 50% alcohol
Solution, 20min is stirred under conditions of 120 turns/min, by the neutral proteinase mixed and excipient in 50 DEG C of conditions
Lower dry 50mim, obtain mucous membrane cleaning agent.
Embodiment 4
The parts by weight of neutral proteinase 15.0, the parts by weight of trypsase 25.0, the parts by weight of tween -86 1.36, Arlacel-80
The parts by weight of 1.14 parts by weight dimethicone, 0.84 parts by weight of silica 0.32, the parts by weight of sodium tetraborate 5.26, boric acid
15.66 parts by weight, the parts by weight of calcium chloride 2.0, the parts by weight of sorbierite 57.
80 mesh sieves are crossed after above-mentioned medicine is weighed, mix 15min, the mass concentration for adding 10 parts by weight is 50% alcohol
Solution, 50min is stirred under conditions of 80 turns/min, by the neutral proteinase mixed and excipient under the conditions of 65 DEG C
20mim is dried, obtains mucous membrane cleaning agent.
Embodiment 5
The parts by weight of neutral proteinase 10.0, the parts by weight of trypsase 20.0, the parts by weight of Tween-80 0.18, Arlacel-80
The parts by weight of 0.18 parts by weight dimethicone, 0.06 parts by weight of silica 0.06, the parts by weight of disodium hydrogen phosphate 50.69, boric acid
4.31 parts by weight, the parts by weight of calcium chloride 2.0, the parts by weight of sorbierite 58.
80 mesh sieves are crossed after above-mentioned medicine is weighed, mix 15min, the mass concentration for adding 10 parts by weight is 50% alcohol
Solution, 30min is stirred under conditions of 60 turns/min, by the neutral proteinase mixed and excipient under the conditions of 65 DEG C
20mim is dried, obtains mucous membrane cleaning agent.
Embodiment 6
The parts by weight of neutral proteinase 30.0, the parts by weight of trypsase 5.0, the parts by weight of Tween-80 2.32, Arlacel-80
The parts by weight of 1.43 parts by weight dimethicone, 0.24 parts by weight of silica 0.36, the parts by weight of sodium acid carbonate 10.21, boric acid
10.56 parts by weight, the parts by weight of calcium chloride 1.53, the parts by weight of sorbierite 35.64, the parts by weight of sodium thiosulfate 5.0.
80 mesh sieves are crossed after above-mentioned medicine is weighed, mix 15min, the mass concentration for adding 10 parts by weight is 50% alcohol
Solution, 30min is stirred under conditions of 60 turns/min, by the neutral proteinase mixed and excipient under the conditions of 65 DEG C
20mim is dried, obtains mucous membrane cleaning agent.
Embodiment 7
The parts by weight of neutral proteinase 10.0, the parts by weight of trypsase 30.0, the parts by weight of Tween-80 0.12, Arlacel-80
The parts by weight of 1.76 parts by weight dimethicone, 0.24 parts by weight of silica 0.36, the parts by weight of sodium acid carbonate 10.21, boric acid
10.56 parts by weight, the parts by weight of calcium chloride 0.02, the parts by weight of sorbierite 26.41, the parts by weight of sodium sulfite 1.5, the weight of lactose 5.0
Part.
80 mesh sieves are crossed after above-mentioned medicine is weighed, mix 15min, the mass concentration for adding 10 parts by weight is 50% alcohol
Solution, 30min is stirred under conditions of 60 turns/min, by the neutral proteinase mixed and excipient under the conditions of 65 DEG C
20mim is dried, obtains mucous membrane cleaning agent.
Embodiment 8
The parts by weight of neutral proteinase 5.0, the parts by weight of trypsase 30.0, the parts by weight of Tween-80 1.38, Arlacel-80
The parts by weight of 1.76 parts by weight dimethicone, 0.28 parts by weight of silica 0.36, the parts by weight of sodium acid carbonate 10.21, phosphoric acid hydrogen
The parts by weight of disodium 38.16, the parts by weight of calcium chloride 0.02, the parts by weight of sorbierite 15.36, the weight of sodium sulfite 1.5, part ascorbic acid
1.5 parts by weight, the parts by weight of lactose 1.0, the parts by weight of fructose 1.0
80 mesh sieves are crossed after above-mentioned medicine is weighed, mix 15min, the mass concentration for adding 10 parts by weight is 50% alcohol
Solution, 50min is stirred under conditions of 40 turns/min, by the neutral proteinase mixed and excipient under the conditions of 65 DEG C
20mim is dried, obtains mucous membrane cleaning agent.
The hydrolysis rate of experimental example 1 is tested
Test group：Embodiment 1 prepares alimentary canal mucus cleaning agent
Control group：The pepsin silicone oil prepared according to the embodiment 1 of application for a patent for invention number 201010136337.7 combines
Thing
Experiment material：Mucoprotein
Time：5min、10min、15min、30min、60min
The degradation rate of the different time experimental group of table 1 and control group to mucoprotein
Test result indicates that：Experimental group is within 5min, 10min, 15min, 30min, 60min time to the drop of mucoprotein
Solution rate is respectively 64%, 86%, 100%, 100%, 100%；Control group is in 5min, 10min, 15min, 30min, 60min
It is respectively 12%, 26%, 44%, 55%, 62% to the degradation rate of mucoprotein in time.Experimental group is right within 15min time
The degradation rate of mucoprotein is 100%, and control group only has 62%, therefore this within 60min time to the degradation rate of mucoprotein
Invention, which provides degraded of the alimentary canal mucus cleaning agent to mucoprotein, an obviously advantage, and degradation rate is very fast.Experimental example 2
Stability experiment
Test group：Embodiment 1 prepares alimentary canal mucus cleaning agent
Control group：The pepsin silicone oil prepared according to the embodiment 1 of application for a patent for invention number 201010136337.7 combines
Thing
40 DEG C of temperature, relative humidity 75%
Time：1 month, 3 months, 6 months, 12 months
The different time experimental group of table 2 and control group proteinase activity
| 1 month | 3 months | 6 months | 12 months | |
| Test group | 99% | 98% | 96% | 92% |
| Control group | 96% | 86% | 78% | 53% |
Test result indicates that：Protease enzyme activity is respectively within 1 month, 3 months, 6 months, time of 12 months for experimental group
For 99%, 98%, 96%, 92%；Control group protease enzyme activity point within 1 month, 3 months, 6 months, time of 12 months
Wei 96%, 86%, 78%, 53%.Placed 12 months under conditions of 37 DEG C of temperature, alimentary canal mucus provided by the invention is clear
The enzyme activity of lotion is reduced only by 8%, and the enzyme activity of commercially available alimentary canal mucus cleaning agent have dropped 47%.Therefore the present invention carries
It is highly stable for alimentary canal mucus cleaning agent, have great advantage than current market product.
Embodiments of the invention are described in detail above, but the content is only presently preferred embodiments of the present invention,
It is not to be regarded as the practical range for limiting the present invention.Any changes and modifications in accordance with the scope of the present application,
All should still it belong within the patent covering scope of the present invention.
Claims (10)
- A kind of 1. alimentary canal mucus cleaning agent, it is characterised in that：Including neutral proteinase, trypsase and excipient, neutral egg The optimal pH of white enzyme is 5.5-8.5, and the neutral proteinase generates for fermentation of bacillus subtilis.
- 2. alimentary canal mucus cleaning agent according to claim 1, it is characterised in that：The excipient includes pH regulations Agent, the pH adjusting agent are the one or more in carbonate, phosphate or borate.
- 3. alimentary canal mucus cleaning agent according to claim 2, it is characterised in that：Also include in the excipient stable Agent, the stabilizer are the one or more in 4- formylphenyl boronic acids, calcium chloride or sorbierite.
- 4. alimentary canal mucus cleaning agent according to claim 3, it is characterised in that：The stabilizer is calcium chloride and sorb Alcohol.
- 5. the alimentary canal mucus cleaning agent according to claim 1-4 any one, it is characterised in that：The alimentary canal mucus Cleaning agent includes neutral proteinase 5-30 parts by weight, trypsase 5-30 parts by weight and excipient 10-130 parts by weight.
- 6. alimentary canal mucus cleaning agent according to claim 5, it is characterised in that：The excipient includes pH adjusting agent 5- 55 parts by weight, defoamer 0.2-5.0 parts by weight, stabilizer 4.8-60 parts by weight, antioxidant 0-5 parts by weight, and flavouring 0-5 weights Measure part.
- 7. alimentary canal mucus cleaning agent according to claim 6, it is characterised in that：The stabilizer includes calcium chloride 0.001-2 parts by weight and sorbierite 4.799-58 parts by weight.
- 8. alimentary canal mucus cleaning agent according to claim 6, it is characterised in that：The granularity of the neutral proteinase is The granularity of 40-200 mesh, preferably neutral proteinase is 80-160 mesh.
- A kind of 9. preparation method of the alimentary canal mucus cleaning agent described in claim 1-8 any one, it is characterised in that：Including Following steps：(1) load weighted neutral proteinase and excipient are crossed into 80 mesh sieves, mixes 15-30min afterwards；(2) mass concentration of addition neutral proteinase and excipient gross weight 5-15% parts by weight is 50% alcoholic solution, after Continuous mixing 20-50min；(3) neutral proteinase mixed and excipient are dried into 20-60mim under the conditions of 30-65 DEG C, obtains mucous membrane cleaning Agent.
- 10. the preparation method of alimentary canal mucus cleaning agent according to claim 9, it is characterised in that：In the step (1) It is mixed into and is stirred, stir speed (S.S.) is that 40-120 turns/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610766046.3A CN107789619A (en) | 2016-08-30 | 2016-08-30 | A kind of alimentary canal mucus cleaning agent |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610766046.3A CN107789619A (en) | 2016-08-30 | 2016-08-30 | A kind of alimentary canal mucus cleaning agent |
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| CN107789619A true CN107789619A (en) | 2018-03-13 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1113516A (en) * | 1994-06-14 | 1995-12-20 | 北京市联合大学 | Bioenzyme used for dismounting pictures and the method therefor |
| CN101804199A (en) * | 2010-03-30 | 2010-08-18 | 四川健能制药有限公司 | Pharmaceutical composition used for alimentary canals and application thereof |
-
2016
- 2016-08-30 CN CN201610766046.3A patent/CN107789619A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1113516A (en) * | 1994-06-14 | 1995-12-20 | 北京市联合大学 | Bioenzyme used for dismounting pictures and the method therefor |
| CN101804199A (en) * | 2010-03-30 | 2010-08-18 | 四川健能制药有限公司 | Pharmaceutical composition used for alimentary canals and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 徐锐: "《发酵技术》", 31 January 2016, 重庆大学出版社 * |
| 章永年等: "《液体洗涤剂》", 30 September 2000, 中国轻工业出版社 * |
| 郝利平: "《食品添加剂》", 31 July 2016, 中国农业大学出版社 * |
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