CN107787973A - Application of the rhamnolipid in terms of plant salt stress is alleviated - Google Patents
Application of the rhamnolipid in terms of plant salt stress is alleviated Download PDFInfo
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- CN107787973A CN107787973A CN201711039965.1A CN201711039965A CN107787973A CN 107787973 A CN107787973 A CN 107787973A CN 201711039965 A CN201711039965 A CN 201711039965A CN 107787973 A CN107787973 A CN 107787973A
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- rhamnolipid
- lawn grass
- salt stress
- korea lawn
- relative
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- 150000003839 salts Chemical class 0.000 title claims abstract description 66
- FCBUKWWQSZQDDI-UHFFFAOYSA-N rhamnolipid Chemical compound CCCCCCCC(CC(O)=O)OC(=O)CC(CCCCCCC)OC1OC(C)C(O)C(O)C1OC1C(O)C(O)C(O)C(C)O1 FCBUKWWQSZQDDI-UHFFFAOYSA-N 0.000 title claims abstract description 53
- 241001520823 Zoysia Species 0.000 claims abstract description 81
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 40
- 230000012010 growth Effects 0.000 claims abstract description 32
- 230000006378 damage Effects 0.000 claims abstract description 8
- 241000196324 Embryophyta Species 0.000 claims description 33
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 claims description 7
- 229930186217 Glycolipid Natural products 0.000 claims description 7
- 230000008859 change Effects 0.000 abstract description 22
- 239000002689 soil Substances 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 4
- 240000001102 Zoysia matrella Species 0.000 description 29
- 241001299698 Zoysia sinica Species 0.000 description 29
- 210000004027 cell Anatomy 0.000 description 12
- 230000001965 increasing effect Effects 0.000 description 8
- 238000012545 processing Methods 0.000 description 7
- 210000000170 cell membrane Anatomy 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 4
- 230000003204 osmotic effect Effects 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 244000241235 Citrullus lanatus Species 0.000 description 2
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 2
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 2
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 239000003876 biosurfactant Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000003792 electrolyte Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229940118019 malondialdehyde Drugs 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 229910001415 sodium ion Inorganic materials 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 108010022172 Chitinases Proteins 0.000 description 1
- 102000012286 Chitinases Human genes 0.000 description 1
- 108010001682 Dextranase Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 239000006004 Quartz sand Substances 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000015784 hyperosmotic salinity response Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000019948 ion homeostasis Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000005502 peroxidation Methods 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 229910052761 rare earth metal Inorganic materials 0.000 description 1
- 150000002910 rare earth metals Chemical class 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
Abstract
The present invention is by studying during salt stress and using after rhamnolipid, the change of the relative growth rate, relative water content, relative conductivity and MDA (MDA) content of Korea lawn grass is alleviating the effect of Korea lawn grass salt stress to verify rhamnolipid, it was found that rhamnolipid can significantly improve the relative water content and relative growth rate of Korea lawn grass during salt stress, reduce the relative conductivity and mda content of Korea lawn grass, so as to alleviate harm of the salt stress to Korea lawn grass, so as to provide a kind of new method to alleviate the salt stress of salt-soda soil Korea lawn grass；The optium concentration of the rhamnolipid solution is 0.5g/L.
Description
Technical field
The present invention relates to a kind of new application of rhamnolipid, specifically, the application in terms of Korea lawn grass salt stress is alleviated.
Background technology
Korea lawn grass is frequently grown in warm and moistening coastal area, to illumination requirement strictly, but is had certain shade tolerance, is resisted
Drought, Salt And Alkali Tolerance, it is strong to diseases and insect pests resistance, it is usually used in Ornamental turf, motion lawn and slope protecting grassplot.Korea lawn grass and other plant
Equally, it is grown in open environment, often by the adverse circumstances such as the influence of poor environment, high temperature, low temperature, salt marsh and arid
The growth of Korea lawn grass will be suppressed, decline turf quality.
The saline and alkaline land area about 9.6 × 10 in the whole world8hm2, and there is salt lick 3.47 × 10 in China7hm2, equivalent to arable land
The 1/3 of area.Soil saliferous can excessively suppress plant growth, and the salinization of soil endangers to caused by plant to be embodied in：In cell
Na+Excessive buildup can break intracellular ionic equilibrium, suppress the eubolism in plant cell, make photosynthesis of plant
Ability decline, therefore plant growth can lack nutrient and then be suppressed even final dead；The salinity in salt-soda soil makes soil
Osmotic pressure be higher than the osmotic pressure of root system of plant, cause plant water suction difficult, cause plant dehydration and dry up；Saline Land meeting
The balance of generation and the removing of the active oxygen destroyed in plant, causes free radical largely to accumulate, and peroxidation of membrane lipids occurs,
Destroying cell membrane makes cell organic matter exosmose, so that cell inactivation.Research finds to live by increasing with Exogenous Trehalose
Property oxygen scavenging capacity, alleviate plasma membrane damage, maintain kytoplasm ionic homeostasis so as to improving the salt-resistance of plant.Dressed seed with earth solution
Or field Sprayed Rare Earth solution enhances control ability of the plant cell membrane to Electrolyte Leakage, stabilizes the generation in plant cell
Apologize for having done sth. wrong journey, improve the salt tolerance of plant.During salt stress, H is sprayed2O2Can increase plant soluble protein, can
The Osmotic Adjustment Substances such as dissolubility sugar and proline, improve activities of antioxidant enzymes and the antioxidants such as SOD, CAT, POD and APX
GSH contents, reduce O2 -And H2O2Accumulation, mitigate membrane oxidation injury and the suppressed degree of plant growth, so as to strengthen the resistance to of plant
Salt.
Rhamnolipid is a kind of very important biosurfactant as caused by pseudomonas aeruginosa.Biological surface is lived
Property agent is the metabolite for having certain biochemical activity, by microorganisms such as bacterium, fungi and yeasts in cells in vitro or
Produce on cell membrane, can also be obtained by the biochemical reactions of animals and plants.Biosurfactant have degradable, toxicity is low,
Source is wide, with the features such as biocompatibility is high, functional group's species is more, adaptable, can put forward the hydrophobic ability of cell surface
Height, increase the direct physical action between microsolubility substrate and plant cell.Research finds that rhamnolipid can not only change cell
Surface nature, increase Surface hydrophobicity of cell, moreover it is possible to significantly improve the solubility of hydrocarbon compound.It is another studies have found that, biological surface
Activating agent can influence the ability of regulation and control of the absorption and desorption of the antibacterial ability and microorganism of microorganism to plant cell membrane, to a huge sum of money
Category combination and the formation of biomembrane etc. have significant impact.Research of the rhamnolipid to watermelon blight preventive and therapeutic effect demonstrates mouse
Lee's glycolipid can prevent and treat watermelon seedlings droop under control environment by inducing plant resistance, studies have found that rhamnolipid pours
Filling processing can improve the activity of chitinase and dextranase in plant.But its work in terms of plant salt stress is alleviated
With being not reported so far.
The content of the invention
The present invention is the relative growth rate of Korea lawn grass, relative by studying during salt stress and using after rhamnolipid
The change of water content, relative conductivity and MDA (MDA) content is alleviating the work of Korea lawn grass salt stress to verify rhamnolipid
With discovery rhamnolipid can significantly improve the relative water content and relative growth rate of Korea lawn grass during salt stress, reduce plant
Relative conductivity and mda content, so as to alleviating harm of the salt stress to plant, so as to alleviate salt-soda soil Korea lawn grass
Salt stress provides a kind of new method.
Can be by pouring sandlwood specifically, rhamnolipid can be used for alleviating harm of the salt stress to Korea lawn grass, during use
Glycolipid sample is realized, it is preferable that the concentration of the rhamnolipid solution is 0.5g/L, after testing, shows that rhamnolipid can be bright
The aobvious relative water content and relative growth rate for improving Korea lawn grass blade during salt stress, reduce Korea lawn grass relative conductivity and
Mda content.
Brief description of the drawings
Fig. 1 is the change of Korea lawn grass relative growth rate during salt stress in the embodiment of the present invention；
Fig. 2 is the change of Korea lawn grass relative conductivity during salt stress in the embodiment of the present invention；
Fig. 3 is the change of Korea lawn grass leaf water content during salt stress in the embodiment of the present invention；
Fig. 4 is that Korea lawn grass mda content changes during salt stress in the embodiment of the present invention；
Fig. 5 is using the change of Korea lawn grass relative growth rate after rhamnolipid in the embodiment of the present invention；
Fig. 6 is using the change of Korea lawn grass relative conductivity after rhamnolipid in the embodiment of the present invention；
Fig. 7 is using the change of Korea lawn grass leaf water content after rhamnolipid in the embodiment of the present invention；
Fig. 8 is using the change of Korea lawn grass mda content after rhamnolipid in the embodiment of the present invention；
In figure：Different lowercase significant differences (P ＜ 0.05).
Embodiment
In order that objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further
Describe in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair
It is bright.
The applicant obtains by many experiments：When the concentration of rhamnolipid is 0.5g/L, it alleviates Korea lawn grass salt stress
Effect it is best, therefore, in following examples from concentration be 0.5g/L rhamnolipid as experiment reagent.
Embodiment
Embodiment takes the divot of two kinds of Korea lawn grass (a diameter of using manilagrass and Zoysia sinica as material
12.00cm) plant in being not easy in permeable flowerpot (high 14.00cm, a diameter of 13.00cm of basin mouth), 6 basins of every kind of plantation, wherein 3
Basin is used as salt treatment, and 3 basins are used as control.The matrix of salt stress processing is to be derived from the original soil of Yancheng Seashore, blended, makes its salt dense
Degree (electrical conductivity) is maintained at 0.5% or so, and cultivation matrix is fitted into Culture basin, is compacted, divot is planted in cultivation matrix
On, the salt-free loam culture of control.Cultivated in room temperature (20 ± 5 DEG C of cultivation temperature), pour 600mL water weekly.Cultivation
Each cone material is trimmed after end, it is taken blade away from basin mouth 2.00cm, 4d, 8d, 12d, 16d and 20d after cultivation
Determine physical signs.It is followed by subsequent processing every basin and pours the rhamnolipid that 100mL concentration is 0.5g/L, 4d, 8d, 12d after application,
16d and 20d takes measuring blade physical signs again.
Physiological index determining method：
1st, relative growth rate
The vertical-growth height of every part of Korea lawn grass is measured, it is high that the vertical-growth of each group processing is highly removed control group growth
Degree, obtained result is relative growth rate.
2nd, leaf r elative water content
The blade of clip manilagrass and Zoysia sinica is some, is quickly put into the aluminium box of known weight, weighs knot
Careless leaf tissue fresh weight in detail.After having claimed plant tissue fresh weight, Korea lawn grass blade is immersed in distilled water and puts 5-9h, until Korea lawn grass
Then Korea lawn grass blade is put into 105 DEG C of baking oven the 15min that finishes, is then placed on 80 DEG C by leaf water close to saturation state
Baking oven in dry to constant weight, weigh tissue dry weight.Leaf r elative water content is calculated using below equation：
Relative water content=(plant tissue fresh weight-plant tissue dry weight)/(plant tissue saturation fresh weight-plant tissue is done
Weight)
3rd, relative conductivity assay method
The Korea lawn grass blade of same position is taken, 0.6cm segment is cut into scissors, is rapidly rushed twice with distilled water, then
Korea lawn grass blade suck dry moisture is wrapped up with blotting paper, the Korea lawn grass blade after 0.3g processing is weighed and is put into thick test tube and adds distilled water
6mL, vacuum infiltration, it is evacuated and deflates 3 times, allow water to be closely contacted with blade and electrolyte is easily oozed out, taken after 30min
Go out, Korea lawn grass is placed and stands 2h at room temperature, then electric conductivity value is determined with conductivity meter, be former electric conductivity value, at Korea lawn grass blade
5min is heated at 100 DEG C to kill, it is cooled to room temperature, then surveys electric conductivity value, is as a result total electric conductivity value.Calculated using following equation
Blade relative conductivity.
Relative conductivity (%)=former electric conductivity value/total electric conductivity value × 100%
4th, MDA (MDA) content assaying method
The Korea lawn grass blade 0.5g shredded is weighed, the monoxones of 2mL 10% (TCA) and a small amount of quartz sand is added, is ground to even
Slurry, then add appropriate TCA further to grind, homogenate is transferred in 5mL centrifuge tubes, centrifuges 10min in 4000rpm, supernatant is sample
Extract solution.The supernatant 2mL (blank adds 2mL distilled water) of centrifugation is drawn, it is molten to add 2mL 0.6%TBA (thiobarbituricacidα-)
Liquid, homomixture centrifuge again in reacting 15min on boiling water bath after rapid cooling.Supernatant is taken to determine 532nm, 600nm and 450nm
Optical density under wavelength.
MDA contents (μm ol/gFW)={ MDA concentration (μm ol/L) × extracting liquid volume (mL) }/plant tissue fresh weight (g)
As a result with analysis
Influence of the salt stress to two kinds of Korea lawn grass relative growth rates
During salt stress, Fig. 1 is shown in the change of two kinds of Korea lawn grass relative growth rates.It will be seen from figure 1 that at the beginning of salt stress
Phase, i.e., from 4d to 8d when, the relative growth rate of manilagrass and Zoysia sinica rises, manilagrass it is relative
Growth rate rises 4%, and the relative growth rate of Zoysia sinica rises 3%, shows the salt stress of short time and contributes to knot thread
The growth of grass, but two kinds of Korea lawn grass are compared without significant difference (P ＞ 0.05).With the extension of salt stress time after 8d, two kinds
The relative growth rate of Korea lawn grass is in be gradually reduced trend.The relative growth rate excursion of manilagrass and Zoysia sinica
Respectively 99%-93% and 97%-89%；It is notable in 12d, 16d and 20d of salt stress, the relative growth rate of Zoysia sinica
Less than the relative growth rate (P ＜ 0.05) of manilagrass, illustrate the relatively raw of two kinds of Korea lawn grass of salt stress significant impact
Long rate.
Influence of the salt stress to two kinds of Korea lawn grass blade relative conductivities
During salt stress, the relative conductivity of two kinds of Korea lawn grass is shown in Fig. 2, figure it is seen that control is relatively electric
Conductance change is little, and without significant difference (P ＞ 0.05).The blade relative conductivity of two kinds of Korea lawn grass is in during salt stress
The excursion of the relative conductivity of the trend being now gradually increasing, manilagrass and Zoysia sinica blade is respectively 8.9%-
33.26% and 9.21%-40.85%.In the 4d of salt stress, though manilagrass and Zoysia sinica relative conductivity have
Rise, but with compareing no significant difference, also without significant difference (P ＞ 0.05) between processing.In 8d, manilagrass is relative
Electrical conductivity is not significantly different (P ＞ 0.05) compared with the control, but the relative conductivity of Zoysia sinica is significantly higher than ditch
The relative conductivity (P ＜ 0.05) of leaf Korea lawn grass.In 12d, 16d and 20d of salt stress, the relative conductivity of two kinds of Korea lawn grass
Not only it is significantly higher than control, and the relative conductivity of Zoysia sinica is significantly higher than relative conductivity (the P ＜ of manilagrass
0.05)。
Influence of the salt stress to two kinds of Korea lawn grass leaf water contents
During salt stress, the leaf water content of two kinds of Korea lawn grass is shown in Fig. 3, and as can be seen from Figure 3, the leaf water content of control becomes
Change less, between the two without significant difference (P ＞ 0.05).During salt stress, the leaf r elative water content of two kinds of Korea lawn grass is in
It is gradually reduced trend, the excursion of the relative water content of manilagrass and Zoysia sinica blade is respectively 78.45%-
55.74% and 76.38%-42.62%.For Zoysia sinica since 4d, relative water content, which is substantially less than, compares (P ＜
0.05), and manilagrass since 8d, relative water content, which is substantially less than, compares (P ＜ 0.05), is handled in 16d and 20d
Manilagrass relative water content is noticeably greater than the relative water content (P ＜ 0.05) of Zoysia sinica.
Influence of the salt stress to two kinds of Korea lawn grass mda contents
During salt stress, Fig. 4 is shown in two kinds of Korea lawn grass mda content changes, as can be seen from Figure 4, control group mda content
Change is little, between the two without significant difference (P ＞ 0.05).The mda content of two kinds of Korea lawn grass is in gradual during salt stress
The mda content excursion of ascendant trend, manilagrass and Zoysia sinica blade is respectively 40 μm of oL/g-190 μ
MoL/g and 45 μm of oL/g-240 μm of oL/g.In the 4d and 8d of salt stress, manilagrass and Zoysia sinica MDA
Content is with compareing without significant difference, also without significant difference (P ＞ 0.05) between processing.Since 12d, manilagrass is with
Magnificent Korea lawn grass mda content is significantly higher than control (P ＜ 0.05), and Zoysia sinica mda content is noticeably greater than ditch leaf
The mda content (P ＜ 0.05) of Korea lawn grass.
Using the change of rhamnolipid latter two Korea lawn grass relative growth rate
Fig. 5 is shown in change using rhamnolipid latter two Korea lawn grass blade relative growth rate, as can be seen from Figure 5 applies
The relative growth rate of rhamnolipid latter two Korea lawn grass is constantly raising, and the blade of manilagrass and Zoysia sinica is relatively raw
The excursion of long rate is respectively 94%-98% and 90%-97%.Apply rhamnolipid 20d when, manilagrass it is relative
Growth rate is than increasing 5% during salt stress 20d, the relative growth rate of Zoysia sinica during salt stress 20d than increasing 8%.
Using rhamnolipid 4d, 8d, 12d and 16d when, the relative growth rate of manilagrass is noticeably greater than the relative of Zoysia sinica
Growth rate (P ＜ 0.05).In 20d, manilagrass and Zoysia sinica blade relative growth rate are not significantly different (P ＞
0.05)。
Using the change of rhamnolipid latter two Korea lawn grass blade relative conductivity
Fig. 6 is shown in change using rhamnolipid latter two Korea lawn grass blade relative conductivity, as can be seen from Figure 6 using mouse
The trend that the blade relative conductivity presentation of Lee glycolipid latter two Korea lawn grass is gradually reduced, manilagrass and Zoysia sinica
The excursion of blade relative conductivity is respectively 33.26%-12.54% and 40.85%-15.42%.Using rhamnolipid
During 20d, for manilagrass blade relative conductivity than reducing 20.72% during salt stress 20d, Zoysia sinica blade is relatively electric
Conductance during salt stress 20d than reducing 25.43%.When applying 4d, 8d, 12d and 16d of rhamnolipid, two kinds of Korea lawn grass
Relative conductivity significant difference (P ＜ 0.05), but in 20d manilagrass and Zoysia sinica blade relative conductivity without
Significant difference (P ＞ 0.05).
Using the change of rhamnolipid latter two Korea lawn grass leaf water content
Fig. 7 is shown in change using rhamnolipid latter two Korea lawn grass leaf r elative water content, as can be seen from Figure 7 using mouse
The leaf r elative water content of Lee glycolipid latter two Korea lawn grass in being gradually increasing trend, manilagrass and Zoysia sinica blade
The excursion of relative water content is respectively 55.74%-73.84% and 42.62%-70.56%.Applying rhamnolipid 20d
When, for manilagrass leaf r elative water content than increasing 18.1% during salt stress 20d, Zoysia sinica relative water content compares salt
27.94% is increased when coercing 20d.When applying 4d, 8d and 12d of rhamnolipid, the relative water content of two portions of Korea lawn grass is poor
Different notable (P ＜ 0.05), but manilagrass and Zoysia sinica leaf r elative water content are without notable in 16d and 20d
Difference (P ＞ 0.05).
Using the change of rhamnolipid latter two Korea lawn grass mda content
Fig. 8 is shown in change using rhamnolipid latter two Korea lawn grass leaf malondialdehyde content, as can be seen from Figure 8 applies
The mda content of rhamnolipid latter two Korea lawn grass constantly reduces, and the blade MDA of manilagrass and Zoysia sinica contains
The excursion of amount is respectively 190 μm oL/g-86 μm oL/g and 240 μm of oL/g-144 μm of oL/g.Applying rhamnolipid 20d
When, the mda content of manilagrass blade is than reducing 54.7% during salt stress 20d, the MDA of Zoysia sinica blade
Content during salt stress 20d than reducing 52.5%.The mda content of manilagrass is notable all the time after rhamnolipid is applied
Less than Zoysia sinica leaf malondialdehyde content (P ＜ 0.05).
It can be seen that significantly rising using the relative water content of rhamnolipid latter two Korea lawn grass, rhamnolipid may pass through rush
Enter the synthesis of the macromolecular such as protein, carbohydrate in Korea lawn grass root cell so as to increase the osmotic potential of Korea lawn grass root cell, make
Korea lawn grass can be absorbed to moisture from soil, and add the transport toward cauline leaf.Using the phase of rhamnolipid latter two Korea lawn grass
In rising trend to growth rate, this is probably that rhamnolipid is absorbed by reducing intracellular sodium ion, is increased to potassium in soil
The absorption of ion, promote photosynthesis, so as to slow down the inhibitory action that salt stress grows to Korea lawn grass.After rhamnolipid,
The increase of leaf water content, the generation and removing for making active oxygen in leaves tend to balance, and membrane lipid peroxidation degree reduces, MDA contents
Also decline therewith.
In summary, show that rhamnolipid can significantly improve the relative water content of two kinds of Korea lawn grass blades during salt stress
And relative growth rate, the relative conductivity and mda content of two kinds of Korea lawn grass are reduced, alleviates harm of the salt stress to Korea lawn grass.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (4)
1. application of the rhamnolipid in terms of plant salt stress is alleviated, it is characterised in that can be with by pouring rhamnolipid solution
Alleviate harm of the salt stress to plant.
2. application of the rhamnolipid as claimed in claim 1 in terms of plant salt stress is alleviated, it is characterised in that the sandlwood
The concentration of glycolipid sample is 0.5g/L.
3. application of the rhamnolipid as claimed in claim 1 in terms of plant salt stress is alleviated, it is characterised in that the sandlwood
Glycolipid can be used for alleviating harm of the salt stress to Korea lawn grass.
4. application of the rhamnolipid as claimed in claim 3 in terms of plant salt stress is alleviated, it is characterised in that the sandlwood
Glycolipid can significantly improve the relative water content and relative growth rate of Korea lawn grass blade during salt stress, reduce the relative of Korea lawn grass
Electrical conductivity and mda content.
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Cited By (6)
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| CN109938044A (en) * | 2019-03-20 | 2019-06-28 | 安徽农业大学 | A kind of medicament that Wheat in Grain Filling Stage high temperature damage is effectively relieved and method |
| CN115039533A (en) * | 2022-06-23 | 2022-09-13 | 山东农业大学 | Orchard compact soil improvement method |
| CN116548445A (en) * | 2023-05-10 | 2023-08-08 | 广东省科学院南繁种业研究所 | Preparation and method for improving cold resistance of tobacco |
| CN116891862A (en) * | 2023-08-22 | 2023-10-17 | 江苏省中国科学院植物研究所 | Zoysia japonica salt tolerance gene ZmLA1, protein and application thereof |
| CN117296595A (en) * | 2023-10-25 | 2023-12-29 | 玉林师范学院 | Application of 2,4-epibrassinolide in promoting growth of Yulan in salt stress and method for relieving Yulan salt stress toxicity |
| CN116199540B (en) * | 2023-01-28 | 2024-03-22 | 西北农林科技大学 | Amino acid-rhamnolipid mixed solution for improving salt tolerance of crops as well as preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109938044A (en) * | 2019-03-20 | 2019-06-28 | 安徽农业大学 | A kind of medicament that Wheat in Grain Filling Stage high temperature damage is effectively relieved and method |
| CN115039533A (en) * | 2022-06-23 | 2022-09-13 | 山东农业大学 | Orchard compact soil improvement method |
| CN116199540B (en) * | 2023-01-28 | 2024-03-22 | 西北农林科技大学 | Amino acid-rhamnolipid mixed solution for improving salt tolerance of crops as well as preparation method and application thereof |
| CN116548445A (en) * | 2023-05-10 | 2023-08-08 | 广东省科学院南繁种业研究所 | Preparation and method for improving cold resistance of tobacco |
| CN116548445B (en) * | 2023-05-10 | 2023-12-08 | 广东省科学院南繁种业研究所 | Preparation and method for improving cold resistance of tobacco |
| CN116891862A (en) * | 2023-08-22 | 2023-10-17 | 江苏省中国科学院植物研究所 | Zoysia japonica salt tolerance gene ZmLA1, protein and application thereof |
| CN116891862B (en) * | 2023-08-22 | 2024-01-30 | 江苏省中国科学院植物研究所 | Zoysia japonica salt tolerance gene ZmLA1, protein and application thereof |
| CN117296595A (en) * | 2023-10-25 | 2023-12-29 | 玉林师范学院 | Application of 2,4-epibrassinolide in promoting growth of Yulan in salt stress and method for relieving Yulan salt stress toxicity |
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