CN107782826A - A kind of high density supermolecule single liquid drop micro-extractor and application - Google Patents
A kind of high density supermolecule single liquid drop micro-extractor and application Download PDFInfo
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- CN107782826A CN107782826A CN201710911581.8A CN201710911581A CN107782826A CN 107782826 A CN107782826 A CN 107782826A CN 201710911581 A CN201710911581 A CN 201710911581A CN 107782826 A CN107782826 A CN 107782826A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
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Abstract
The invention provides a kind of high density supermolecule single liquid drop micro-extractor, including extraction flask, drop lifting apparatus is provided with the extraction flask, the drop lifting apparatus includes lifting base and support meanss, support meanss one end, which is connected to, lifts on base, and the other end is connected to extraction flask.Device provided by the invention can be used for the separation and concentration of trace materials and analysis, substantially step in the complex matrices samples such as environment, medicine, food as follows：(1) environmental water sample to be extracted is added in extraction flask and be placed on magnetic stirring apparatus；(2) extractant is drawn using micro syringe to be injected into the circular groove of lifting apparatus；(3) open magnetic stirring apparatus and temperature and rotating speed are set, extracted；(4) drop is reclaimed after extracting, high performance liquid chromatograph can be directly injected into and analyzed.Apparatus of the present invention making is simple, cost is cheap, and extracting operation is convenient, consumption of organic solvent is few, and detection cycle is short, and extracting and enriching effect is preferable.
Description
Technical field
The invention belongs to Sample Pretreatment Technique Used field in analytical chemistry, and in particular to a kind of high density supermolecule list drop
Micro-extraction device and its application.
Background technology
Single-drop microextraction technology is the micro- extraction of a kind of drop that Cantwell and Dasgupta et al. put forward in 1996
Technology is taken, and is developed rapidly in recent years.Its most common application model is to drip water-insoluble extractant by single
Immerse sample solution in or after ullage, a period of time reclaim drop go forward side by side sample analysis.The principle of extraction is based on treating
Distribution of the thing in aqueous phase and extraction phase is surveyed, because sample and droplet size differ greatly, thus higher enrichment effect can be obtained
Fruit.At present, the technology has been successfully applied to organic compound in the complex matrices sample such as environmental sample, food, biological sample
Extraction and enrichment.However, single droplet extraction and the combination of high performance liquid chromatography (HPLC) are constantly subjected to limit, because single drop
Extractant typically from the organic solvent such as toluene, hexane, isooctane and carbon tetrachloride, these solvents and reversed-phase liquid chromatography
Mobile phase is incompatible.Therefore, new extractant is found, turns into the important topic that single droplet extraction faces.
Supermolecule solvent be surfactant under certain inducement (such as temperature, salt, organic solvent) in the aqueous solution
In the aggregation that is self-assembly of.It is a kind of water-insoluble extractant, can be compatible with the mobile phase of reversed-phase liquid chromatography,
With it is inexpensive, be easy to get, the characteristic such as environmental protection, it is of some concern in recent years.Compared with traditional extractant, supermolecule solvent sticks
Larger, relatively large beneficial to volume is formed stable droplet is spent, and the support bar with hanging drop has stronger affinity；Its
Secondary, supermolecule solvent is non-volatile extractant, and physicochemical properties are relatively stable, can be at a higher temperature to target
Thing is extracted；In addition, different from the extraction power that organic solvent is single, supermolecule solvent can provide for object to be dredged
The multi-acting forces such as water, electrostatic, π-cation, hydrogen bond, there is preferable extraction to the object of opposed polarity.Therefore,
Supermolecule solvent Single-drop microextraction method has broad application prospects.
But a difficult point facing in actual applications of Single-drop microextraction is the unstability of single drop.Single drop is micro-
Conventional supporter of the gas chromatographic sample introduction pin as single drop of extraction, it is because gas chromatographic sample introduction pin has a larger inclination
Face, the contact area with extractant can be increased, improve the stability of extraction drop, the volume of increase extraction drop.In addition,
One section of teflon rod or the plastics square embedded with stria can also be installed on the syringe needle of sample introduction needle, to increase supporter
With the contact area of extractant, the stability of drop is improved.
Early-stage Study finds that hexafluoroisopropanol is a kind of solvent-induced dose of supermolecule with universality, and it can make non-
It is molten that ionic surfactant, ionic surfactant and its compound system etc. form supermolecule in very wide condition and range
Agent.The supermolecule solvent of hexafluoroisopropanol mediation has the characteristics that：Phase splitting at room temperature (liquid-liquid two-phase)；Supermolecule solvent is because close
Degree is big and is located at system lower floor, and dosage is small during extraction；The condition and range of split-phase is wide etc..These characteristics compensate for existing super well
The deficiencies of molecular solvent condition of phase separation is harsh, liquid-liquid two phase region is narrow, micro low-density supermolecule solvent phase hardly possible is collected.But
It is, if the high density supermolecule solvent of hexafluoroisopropanol mediation is using Single-drop microextraction mode, traditional suspension type list
The droplet size that droplet extraction device is resistant to is smaller (being less than 10 μ L), and drop is extremely unstable, is heating or is stirring
Under the conditions of, drop meeting heavy losses even drop.Therefore, the high density supermolecule list drop of hexafluoroisopropanol mediation is as extraction
Agent lacks a kind of matched extraction equipment, to improve the stability of drop, reduces loss of the drop in extraction process.
The content of the invention
The present invention in view of the deficienciess of the prior art, provide a kind of high density supermolecule single liquid drop micro-extractor and its
Application process, to improve the stability of single drop and extraction efficiency.
Concrete scheme provided by the invention is as follows.
A kind of high density supermolecule single liquid drop micro-extractor, including extraction flask, drop is provided with the extraction flask and is lifted
Device.
A progressive ground.The drop lifting apparatus includes lifting base and support meanss, support meanss one end connection
In lifting on base, the other end is connected to extraction flask.
A progressive ground.The base of lifting is combined by two different cylinders of diameter, above cylinder diameter it is big
In following cylinder, base upper surface is provided with circular groove.
A progressive ground.The support meanss are the metallic rod that can arbitrarily bend.
A progressive ground.The extraction flask is made up of glass material.
A kind of application process of above-specified high density supermolecule single liquid drop micro-extractor, comprises the following steps：
(1) environmental water sample to be extracted is added in extraction flask, adds magnetic stir bar；
(2) thermostat water bath is placed on magnetic stirring apparatus, extraction flask is put into after adding water；
(3) adjustment lifting apparatus is at vertical position and is immersed in water sample to be extracted, is inhaled using micro syringe
Take extractant to be injected into the groove of lifting apparatus, extractant is formed hemispherical drop in lifting apparatus upper surface；
(4) open magnetic stirring apparatus and temperature and rotating speed are set, extracted；
(5) after extracting, magnetic stirring apparatus power supply is closed, the drop recovery finished will be extracted using micro syringe；
(6) drop of recovery is directly injected into high performance liquid chromatograph to be analyzed to obtain test result.
Above-mentioned extractant is high density supermolecule list droplet extraction agent, and its composition includes anion surfactant laurate
(LA), cationic surfactant trimethyl ammonium hydroxide (DTAOH), hexafluoroisopropanol and water.
Above-mentioned extractant is by anion surfactant laurate (LA) and cationic surfactant dodecyl three
Ammonium hydroxide (DTAOH) is dissolved in hexafluoroisopropanol, is added water and is well mixed, and is protected after forming liquid-liquid diphase after centrifugation
The lower floor's solution stayed.
Above-mentioned thermostat water bath is made up of glass material.
Usefulness of the present invention：
(1) design of lifting apparatus adds stability of single drop in extraction process；
(2) lifting apparatus makes single droplet formation hemispherical, adds the volume and contact area of extractant, improves extraction
Efficiency；
(3) extractant has the characteristics of high density, stability are good, is lost in extraction process small, and dosage is few；
(4) extractant can be directly injected into high performance liquid chromatograph after extraction and be analyzed, and shorten test period；
(5) extractant contains cationic and anionic surfactant, suitable for the extraction of the compound of opposed polarity, for hydrophobic
Property, the extracting and enriching effect of neutrality and polar substances it is preferable.
Brief description of the drawings
Fig. 1 is high density supermolecule single liquid drop micro-extractor structural representation of the present invention；
Fig. 2 a are the side view of drop lifting apparatus center base of the present invention, and Fig. 2 b are drop lifting apparatus center base of the present invention
Top view；
Fig. 3 is the preparation process schematic diagram of high density supermolecule solvent；
Fig. 4 is Single-drop microextraction process schematic；
Before the high density supermolecule Single-drop microextraction that Fig. 5 mediates for 100ng/mL mark-ons East Lake water sample through hexafluoroisopropanol
High-efficient liquid phase chromatogram afterwards, curve a are the collection of illustrative plates after extraction, and curve b is the collection of illustrative plates before extraction, and curve c is blank supermolecule
The collection of illustrative plates of solvent, wherein peak 1,2,3,4,5,6 represent sulphadiazine, Norfloxacin, Ciprofloxacin, Danofloxacin, sulfanilamide (SN) respectively
Diformazan pyrimidine, Enrofloxacin.
Reference：1- extraction flasks, 2- extractants, 3- lift base, 4- support meanss, 5- magnetic stir bars, 6- constant temperature
Water-bath, 7- magnetic stirring apparatus.
Embodiment
The present invention is further described below in conjunction with the accompanying drawings, the complete not limited to this of present disclosure.
A kind of high density supermolecule single liquid drop micro-extractor, as shown in figure 1, including extraction flask 1, the extraction flask 1 is by saturating
Bright glass is made, and is provided with drop lifting apparatus.Drop lifting apparatus includes lifting base 3 and support meanss 4.Lift bottom
Seat 3 is combined by two different cylinders of diameter, above cylinder diameter be more than following cylinder, base upper surface is provided with
Circular groove, as shown in Figure 2, it is preferred that cylinder 1.5mm × Φ 4mm above, below cylinder 3.5mm × Φ 3mm, it is circular
Groove 0.25mm × Φ 2mm.Support meanss 4 are the metallic rod that can arbitrarily bend, and its one end, which is connected to, lifts on base 3, another
End is connected to extraction flask 1.
A kind of application process of high density supermolecule single liquid drop micro-extractor, comprises the following steps：
(1) 20mL environmental water samples to be extracted are added in extraction flask, adds magnetic stir bar；
(2) thermostat water bath is placed on magnetic stirring apparatus, extraction flask is put into after adding water；
(3) adjustment lifting apparatus is at vertical position and is immersed in water sample to be extracted, micro- using 50 μ L or 100 μ L
Amount syringe is drawn extractant and is injected into the groove of lifting apparatus, extractant is formed hemispherical liquid in lifting apparatus upper surface
Drop；
(4) open magnetic stirring apparatus and temperature and rotating speed are set, extracted；
(5) after extracting, magnetic stirring apparatus power supply is closed, the drop recovery finished will be extracted using micro syringe；
(6) drop of recovery is directly injected into high performance liquid chromatograph to be analyzed to obtain test result.
Fig. 4 shows single droplet extraction process.
The preparation of high density supermolecule solvent, as shown in figure 3, specific as follows：
By 70mg anion surfactant laurate solid and 36.75mg cationic surfactant dodecyl
Trimethylammonium hydroxide solid mixes, and adds 500 μ L hexafluoroisopropanol ultrasonic dissolution, adds 5mL water and mixes, with 3000rpm
Interface is obtained after centrifugation 5min clearly two layers, and upper strata is aqueous phase, and lower floor is supermolecule solvent phase, and lower floor's solvent of reservation is mutually made
For extractant.
Before the high density supermolecule Single-drop microextraction that Fig. 5 mediates for 100ng/mL mark-ons East Lake water sample through hexafluoroisopropanol
High-efficient liquid phase chromatogram afterwards, curve a are the collection of illustrative plates after extraction, and curve b is the collection of illustrative plates before extraction, and curve c is blank supermolecule
The collection of illustrative plates of solvent, wherein peak 1,2,3,4,5,6 represent sulphadiazine, Norfloxacin, Ciprofloxacin, Danofloxacin, sulfanilamide (SN) respectively
Diformazan pyrimidine, Enrofloxacin.
In the above-described embodiments, preparing the system condition of supermolecule solvent, (including cationic and anionic surfactant is total dense
Degree and ratio, hexafluoroisopropanol addition) and extraction conditions (including droplet size, extraction time, temperature, rotor speed, wait to extract
Water sampling pH) it can be adjusted to realize the efficiently concentrating to target analytes.Tables 1 and 2 is that supermolecule list drop is micro-
The Method validation result of sulfanilamide (SN) and FQNS in extraction-HPLC combination detection East Lake water samples.Can by data in table
To find out, after introducing the extraction equipment, 6 kinds of analytes are in good linear relationship in 10~100ng/mL concentration range,
Coefficient correlation is all higher than 0.9969, and the detections of two kinds of sulfa drugs and four kinds of FQNSs is limited to 0.81~
3.16ng/mL, RSD is below 7.24% in a few days, in the daytime, the rate of recovery of three kinds of spiked levels between 81.2~106.3%, by
This explanation this method test limit is low, and the degree of accuracy is high, and reproducible and easy to operate, analysis time is short, and solvent load is few, ring
Border is friendly, as a kind of new sample pre-treatments analysis method, has huge potentiality.
The linear relationship of table 1 and test limit
The degree of accuracy of table 2 and precision
The foregoing is only a preferred embodiment of the present invention, but the scope of protection of the invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in any modification for being made, equivalent substitution and
Improve etc., it should be included within the protection domain of invention.
Claims (9)
1. a kind of high density supermolecule single liquid drop micro-extractor, including extraction flask (1) is characterized in that：The extraction flask (1)
On be provided with drop lifting apparatus.
2. high density supermolecule single liquid drop micro-extractor according to claim 1, it is characterised in that：The drop is lifted
Device includes lifting base (3) and support meanss (4), and described support meanss (4) one end, which is connected to, lifts on base (3), another
End is connected to extraction flask (1).
3. high density supermolecule single liquid drop micro-extractor according to claim 2, it is characterised in that：It is described to lift base
(3) combined by two different cylinders of diameter, above cylinder diameter be more than following cylinder, base (3) upper surface
It is provided with circular groove.
4. high density supermolecule single liquid drop micro-extractor according to claim 2, it is characterised in that：The support meanss
(4) metallic rod can arbitrarily to bend.
5. high density supermolecule single liquid drop micro-extractor according to claim 2, it is characterised in that：The extraction flask
(1) it is made up of glass material.
A kind of 6. application process of the high density supermolecule single liquid drop micro-extractor described in claim 1, it is characterised in that bag
Include following steps：
(1) environmental water sample to be extracted is added in extraction flask, adds magnetic stir bar；
(2) thermostat water bath is placed on magnetic stirring apparatus, extraction flask is put into after adding water；
(3) adjustment lifting apparatus is at vertical position and is immersed in water sample to be extracted, is drawn and extracted using micro syringe
Take agent to be injected into the circular groove of lifting apparatus, extractant is formed hemispherical drop in lifting apparatus upper surface；
(4) open magnetic stirring apparatus and temperature and rotating speed are set, extracted；
(5) after extracting, magnetic stirring apparatus power supply is closed, the drop finished using micro syringe recovery extraction；
(6) drop of recovery is directly injected into high performance liquid chromatograph to be analyzed to obtain test result.
7. the application process of high density supermolecule single liquid drop micro-extractor according to claim 6, it is characterised in that：Institute
It is high density supermolecule list droplet extraction agent to state extractant, its composition include anion surfactant laurate (LA), sun from
Sub- surfactant trimethyl ammonium hydroxide (DTAOH), hexafluoroisopropanol and water.
8. the application process of high density supermolecule single liquid drop micro-extractor according to claim 6, it is characterised in that：Institute
It is by anion surfactant laurate (LA) and cationic surfactant trimethyl hydroxide to state extractant
Ammonium (DTAOH) is dissolved in hexafluoroisopropanol, is added water and is well mixed, and it is molten that the lower floor retained is formed after liquid-liquid diphase after centrifugation
Liquid.
9. the application process of high density supermolecule single liquid drop micro-extractor according to claim 6, it is characterised in that：Institute
The thermostat water bath stated is made up of glass material.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101196445A (en) * | 2007-07-31 | 2008-06-11 | 中山大学 | High-efficiency single liquid drop micro-extractor and application thereof |
| KR20090030761A (en) * | 2007-09-21 | 2009-03-25 | 재단법인서울대학교산학협력재단 | Dual stacking method combining large volume single drop microextraction and sweeping and the capillary for large volume single drop microextraction |
| WO2010017564A1 (en) * | 2008-08-08 | 2010-02-11 | University Of Toledo | Boron selective ionic liquids and polymeric ionic liquids, methods of making and methods of use thereof |
| WO2011031005A2 (en) * | 2009-09-08 | 2011-03-17 | Snu R & Db Foundation | Single drop microextraction method combined with capillary electrophoresis-mass spectrometry |
| CN203899202U (en) * | 2014-05-08 | 2014-10-29 | 中国水产科学研究院黄海水产研究所 | Ionic liquid drop micro-extraction device |
| CN105617711A (en) * | 2014-10-16 | 2016-06-01 | 中国药科大学 | New technology for separating trace components in complex sample through combination of a class of magnetic ionic liquids and corresponding micro-extraction apparatus |
| CN107158745A (en) * | 2016-10-18 | 2017-09-15 | 四川大学 | A kind of micro-extraction component and super gravity field micro-extraction device and extracting process |
-
2017
- 2017-09-29 CN CN201710911581.8A patent/CN107782826B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101196445A (en) * | 2007-07-31 | 2008-06-11 | 中山大学 | High-efficiency single liquid drop micro-extractor and application thereof |
| KR20090030761A (en) * | 2007-09-21 | 2009-03-25 | 재단법인서울대학교산학협력재단 | Dual stacking method combining large volume single drop microextraction and sweeping and the capillary for large volume single drop microextraction |
| WO2010017564A1 (en) * | 2008-08-08 | 2010-02-11 | University Of Toledo | Boron selective ionic liquids and polymeric ionic liquids, methods of making and methods of use thereof |
| WO2011031005A2 (en) * | 2009-09-08 | 2011-03-17 | Snu R & Db Foundation | Single drop microextraction method combined with capillary electrophoresis-mass spectrometry |
| CN203899202U (en) * | 2014-05-08 | 2014-10-29 | 中国水产科学研究院黄海水产研究所 | Ionic liquid drop micro-extraction device |
| CN105617711A (en) * | 2014-10-16 | 2016-06-01 | 中国药科大学 | New technology for separating trace components in complex sample through combination of a class of magnetic ionic liquids and corresponding micro-extraction apparatus |
| CN107158745A (en) * | 2016-10-18 | 2017-09-15 | 四川大学 | A kind of micro-extraction component and super gravity field micro-extraction device and extracting process |
Non-Patent Citations (5)
| Title |
|---|
| LI XU 等: "Developments in single-drop microextraction", 《JOURNAL OF CHROMATOGRAPHY A》 * |
| WEN-WEN DENG 等: "Hexfluoroisopropanol-Based Deep Eutectic Solvent/Salt Aqueous Two-Phase Systems for Extraction of Anthraquinones from Rhei Radix et Rhizoma Samples", 《ACS SUSTAINABLE CHEM.ENG.》 * |
| YUFENG ZHANG 等: "Liquid phase microextraction using knitting wool as the extractant phase holder before chromatographic analysis: A new approach for trace analysis", 《JOURNAL OF CHROMATOGRAPHY A》 * |
| 王素利 等: "液相微萃取技术在农药残留分析中的应用研究进展", 《农药学学报》 * |
| 邓勃 等: "单滴微萃取的应用进展", 《现代仪器》 * |
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