CN107782777A - A kind of either high redox activity bovine serum albumin(BSA) carbon nano-tube combination electrode and its preparation method and application - Google Patents
A kind of either high redox activity bovine serum albumin(BSA) carbon nano-tube combination electrode and its preparation method and application Download PDFInfo
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Abstract
The invention belongs to the technical field of Biocomposite material, discloses a kind of either high redox activity bovine serum albumin(BSA) carbon nano-tube combination electrode and its preparation method and application.Present invention dispersing Nano carbon tubes (CNTs) under ultrasonic wave and bovine serum albumin(BSA) (BSA) effect, it is added drop-wise on conducting base, BSA CNTs combination electrodes are prepared with after the activation of continuous cyclic voltammetry by drying, and impart non-oxide reduction transporter either high redox activity.The preparation method not only has the characteristics that operation is fast and convenient, raw material is easy to get, cheap environmentally friendly, efficient stable, and has broad application prospects in bioanalysis and electro-catalysis field.
Description
Technical field
The invention belongs to the technical field of Biocomposite material, and in particular to a kind of either high redox activity bovine serum albumin
In vain-carbon nano-tube combination electrode and its preparation method and application.
Background technology
BSA is the transporter in a kind of organism.Its abundance, it is easy to purifying, cost low, can not only be used for synthesizing material
The template of material, also can be as the model protein of research.BSA because being considered as a kind of amphoteric surfactant with carboxyl and amino,
It is commonly used for the dispersant in materials synthesis field.But BSA is a kind of megohmite insulant, and the amino acid such as tryptophan is buried BSA's
In hydrophobic pocket, therefore BSA is more difficult shows redox active in electroanalysis and electro-catalysis.
CNTs is considered as being curled into nanotube-shaped graphene film, has pi-electron delocalization structure and good electric conductivity.
BSA rich in carboxyl and amino can make CNTs dispersed, realize CNTs functionalization and solvation, form BSA-CNTs π
Electron system.For BSA deficiency, the present invention is made up using CNTs structural advantages and satisfactory electrical conductivity, it is desirable to which development is a kind of
Fast and convenient, cheap environmental protection, the preparation method of efficient stable are operated, realizes the redox active of haemocyanin.
The content of the invention
In order to overcome shortcoming and defect present in prior art, primary and foremost purpose of the invention is to provide a kind of high oxidation
The preparation method of reducing activity bovine serum albumin(BSA)-carbon nano-tube combination electrode.This method select have pi-electron delocalization structure and
The CNTs of satisfactory electrical conductivity makes up BSA deficiency, it is desirable to which both combinations can show the redox active of haemocyanin.
Another object of the present invention is to provide a kind of either high redox activity ox being prepared by above-mentioned preparation method
Seralbumin-carbon nano-tube combination electrode.
It is still another object of the present invention to provide a kind of above-mentioned either high redox activity bovine serum albumin(BSA)-CNT is multiple
The application of composite electrode.
The purpose of the present invention is achieved through the following technical solutions：
A kind of preparation method of either high redox activity bovine serum albumin(BSA)-carbon nano-tube combination electrode, including following step
Suddenly：
(1) buffer preparation bovine serum albumin solution is used, then adds single wall carbon into bovine serum albumin solution and receives
Mitron (CNTs)；Under water bath condition, bovine serum albumin(BSA)-carbon nano-tube solution is obtained by the way that ultrasonic wave is scattered；
(2) bovine serum albumin(BSA)-carbon nano-tube solution is added drop-wise on conducting base, room temperature carries out freeze-day with constant temperature after air-drying
And preservation, obtain bovine serum albumin(BSA)-carbon nano-tube combination electrode；
(3) be working electrode by bovine serum albumin(BSA)-carbon nano-tube combination electrode, saturated calomel electrode be reference electrode,
Ti electrode is to electrode, is connected respectively with 3 electrode contacts of electrochemical workstation, then matched somebody with somebody cushioning liquid is added cathode pool
With make electrolyte solution in anode pool；
(4) electrode activation is carried out using cyclic voltammetry.
Step (1) described cushioning liquid is pH 7.2 trishydroxymethylaminomethane/sodium chloride mixed solution, wherein three hydroxyls
The concentration of aminomethane is 0.02molL-1, the concentration of sodium chloride is 0.1molL-1；The bovine serum albumin(BSA) is molten
The concentration of bovine serum albumin(BSA) is 0.1~80 μm of olL in liquid-1；The single-walled carbon nanotube is received in bovine serum albumin(BSA)-carbon
Concentration in mitron solution is 0.1~10 μ gL-1。
Step (1) described water-bath is under conditions of 10~40 DEG C of temperature；The scattered frequency of the ultrasonic wave for 25~
130KHz, 40~100W of power, when a length of 20~300min.
Step (2) described freeze-day with constant temperature is at 25~60 DEG C of temperature, is dried 4~72 hours.
Step (3) described electrolyte solution is pH 7.2 trishydroxymethylaminomethane/sodium chloride buffer solution, wherein three
The concentration of hydroxymethyl aminomethane is 0.02molL-1, the concentration of sodium chloride is 0.1molL-1。
The process that step (4) cyclic voltammetry carries out electrode activation is potential range -0.2~1.0V, sweeps speed 0.01
~0.6V s-1, the scanning circle of the number of turns 2~100.
A kind of either high redox activity bovine serum albumin(BSA)-CNT for being prepared by above-mentioned preparation method is compound
Electrode.
Above-mentioned either high redox activity bovine serum albumin(BSA)-carbon nano-tube combination electrode is led in bioanalysis and electro-catalysis
Application in domain
Compared with prior art, the present invention has advantages below and beneficial effect：
The preparation method of the present invention has the advantages that operation is fast and convenient, raw material is easy to get, cheap environmentally friendly, efficient stable；System
After standby obtained bovine serum albumin(BSA)-carbon nano-tube combination electrode is activated, is shown in 0.1~0.2V and come from BSA itself
Good oxidation reducing activity, therefore have broad application prospects in bioanalysis and electro-catalysis field.
Brief description of the drawings
Fig. 1 is the activation process of BSA-CNTs combination electrodes in the embodiment of the present invention 1.
Fig. 2 is the redox active of BSA-CNTs combination electrodes in the embodiment of the present invention 2.
Embodiment
The present invention is further illustrated below in conjunction with specific embodiments, but the implementation of the present invention is not limited to this.Root
According to purpose of design of the present invention, the simple replacement of allied substances and the change of size shape, such as change carbon nanotube sizes and
Type, change the species of protein, bath temperature, frequency, power and time when changing ultrasonic, change solute dosage or
PH value of solution etc. all should belong to the scope of the present invention；Test method used in following embodiments unless otherwise specified,
For the existing conventional method of the art；Used material, reagent etc., unless otherwise specified, commercially
Arrive.
Embodiment 1：The activation process of BSA-CNTs combination electrodes
The preparation and activation of the present embodiment BSA-CNTs combination electrodes, are realized by the following method：
(1) pH 7.2 0.02molL is prepared using acidometer-1Trishydroxymethylaminomethane/0.1molL-1NaCl
Cushioning liquid, and 10 μm of olL are prepared with this-1Bovine serum albumin(BSA) (BSA) solution；Again toward sequentially adding in cillin bottle
5mLBSA solution and 5mg single-walled carbon nanotubes, under 10 DEG C of water bath conditions, disperse CNTs, supersonic frequency using ultrasonic wave and BSA
For 40KHz, ultrasonic power 99W, when a length of 40min, obtain BSA-CNTs solution
(2) the scattered obtained BSA-CNTs solution of 20 μ L is added dropwise to electro-conductive glass ITO, after room temperature air-dries, constant 40 DEG C
12~20h drying is carried out, obtains bovine serum albumin(BSA)-carbon nano-tube combination electrode (BSA-CNTs combination electrodes).
(3) it is reference electrode using the BSA-CNTs combination electrodes being dried to obtain as working electrode, saturated calomel electrode, titanium electricity
Extremely to electrode, it is connected respectively with 3 electrode contacts of electrochemical workstation；The electrolyte solution of battery is pH's 7.2
0.02mol·L-1Trishydroxymethylaminomethane/0.1molL-1NaCl cushioning liquid.
(4) cyclic voltammetry and arrange parameter are selected：Potential range -0.2~1.0V, sweep fast 0.05V s-1, scan the number of turns
5 circles, as a result as shown in Figure 1；
As seen from Figure 1, at the beginning as current potential is shuffled, there is a current density in 0.79V and reach 0.14mA
cm-1Oxidation peak；During negative sense flyback, there is a current density in 0.12V and reach 0.042mA cm-1Reduction peak；The
Two circles are shuffled with current potential, and a current density occur in 0.19V reaches 0.035mA cm-1Oxidation peak, 0.79V oxidation peak is but
Suddenly reduce；Second circle has about been stablized in 0.04mA to the 5th circle, relatively this negative to the current density of reduction peak and oxidation peak
cm-1With 0.035mA cm-1, 0.79V oxidation peak then continues to reduce.
The redox active of embodiment 2BSA-CNTs combination electrodes
It is as follows to illustrate the redox active after the activation of BSA-CNTs combination electrodes, concrete operation step in embodiment 1：
Match somebody with somebody toward battery addition after cushioning liquid makees electrolyte solution, selection cyclic voltammetry simultaneously arrange parameter：Current potential model
0~0.4V is enclosed, the scanning number of turns 1 is enclosed, and changes sweep degree of hastening 0.05,0.02,0.01,0.005,0.002,0.001V s successively-1,
As a result it is as shown in Figure 2；
As seen from Figure 2, the BSA-CNTs after 0.19V and 0.12V a pair of the redox peaks occurred illustrate activation
Combination electrode has higher redox active.The reduction peak current density of each sweep speed cyclic voltammetry curve is taken to phase
Sweep speed is answered to map, slope k=6.64 × 10 of linear fit-5, both linear correlation (R2=0.994) electrode, is illustrated
Surface reaction rate is very fast, and electrode controls for diffusion.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (8)
- A kind of 1. preparation method of either high redox activity bovine serum albumin(BSA)-carbon nano-tube combination electrode, it is characterised in that bag Include following steps：(1) buffer preparation bovine serum albumin solution is used, then single-walled carbon nanotube is added into bovine serum albumin solution； Under water bath condition, bovine serum albumin(BSA)-carbon nano-tube solution is obtained by the way that ultrasonic wave is scattered；(2) bovine serum albumin(BSA)-carbon nano-tube solution is added drop-wise on conducting base, room temperature carries out freeze-day with constant temperature and guarantor after air-drying Deposit, obtain bovine serum albumin(BSA)-carbon nano-tube combination electrode；(3) be working electrode by bovine serum albumin(BSA)-carbon nano-tube combination electrode, saturated calomel electrode be reference electrode, titanium electricity Extremely to electrode, it is connected respectively with 3 electrode contacts of electrochemical workstation, then matched somebody with somebody cushioning liquid is added cathode pool and sun Make electrolyte solution in pond in pole；(4) electrode activation is carried out using cyclic voltammetry.
- A kind of 2. system of either high redox activity bovine serum albumin(BSA)-carbon nano-tube combination electrode according to claim 1 Preparation Method, it is characterised in that：The trishydroxymethylaminomethane that step (1) described cushioning liquid is pH 7.2/sodium chloride mixing is molten The concentration of liquid, wherein trishydroxymethylaminomethane is 0.02molL-1, the concentration of sodium chloride is 0.1molL-1；The ox blood The concentration of bovine serum albumin(BSA) is 0.1~80 μm of olL in pure protein solution-1；The single-walled carbon nanotube is pure in ox blood Concentration in albumen-carbon nano-tube solution is 0.1~10 μ gL-1。
- A kind of 3. system of either high redox activity bovine serum albumin(BSA)-carbon nano-tube combination electrode according to claim 1 Preparation Method, it is characterised in that：Step (1) described water-bath is under conditions of 10~40 DEG C of temperature；The scattered frequency of the ultrasonic wave Rate is 25~130KHz, 40~100W of power, when a length of 20~300min.
- A kind of 4. system of either high redox activity bovine serum albumin(BSA)-carbon nano-tube combination electrode according to claim 1 Preparation Method, it is characterised in that：Step (2) described freeze-day with constant temperature is at 25~60 DEG C of temperature, is dried 4~72 hours.
- A kind of 5. system of either high redox activity bovine serum albumin(BSA)-carbon nano-tube combination electrode according to claim 1 Preparation Method, it is characterised in that：Step (3) described electrolyte solution is pH 7.2 trishydroxymethylaminomethane/sodium chloride buffer The concentration of solution, wherein trishydroxymethylaminomethane is 0.02molL-1, the concentration of sodium chloride is 0.1molL-1。
- A kind of 6. system of either high redox activity bovine serum albumin(BSA)-carbon nano-tube combination electrode according to claim 1 Preparation Method, it is characterised in that：Step (4) cyclic voltammetry carry out the process of electrode activation for potential range -0.2~ 1.0V, sweep 0.01~0.6Vs of speed-1, the scanning circle of the number of turns 2~100.
- 7. either high redox activity bovine serum albumin(BSA)-carbon that a kind of preparation method as described in claim 1 is prepared is received Mitron combination electrode.
- 8. either high redox activity bovine serum albumin(BSA)-carbon nano-tube combination electrode according to claim 7 is in biology point Application in analysis and electro-catalysis field.
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Cited By (2)
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| CN110057898A (en) * | 2019-04-15 | 2019-07-26 | 华南师范大学 | A kind of electrochemical detection method of high concentration denaturation bovine serum albumin(BSA) |
| CN114904538A (en) * | 2022-06-09 | 2022-08-16 | 浙江工业大学 | Biomass charcoal-loaded palladium/copper cobaltate composite material and preparation method and application thereof |
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Application publication date: 20180309 |