CN107782682A - Method based on the ammonia-nitrogen content determination efficiency in increasing water quality - Google Patents
Method based on the ammonia-nitrogen content determination efficiency in increasing water quality Download PDFInfo
- Publication number
- CN107782682A CN107782682A CN201610732225.5A CN201610732225A CN107782682A CN 107782682 A CN107782682 A CN 107782682A CN 201610732225 A CN201610732225 A CN 201610732225A CN 107782682 A CN107782682 A CN 107782682A
- Authority
- CN
- China
- Prior art keywords
- water
- ammonia
- add
- absorbance
- diluted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/3103—Atomic absorption analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a kind of method of the ammonia-nitrogen content determination efficiency based in increasing water quality, 20g KIs are weighed to be dissolved in about 100ml water, it is a small amount of by several times while stirring to add mercuric chloride crystalline powder, to when vermilion precipitation occur and not readily dissolving, it is changed to that saturation liquor hydrargyri perchloridi is added dropwise；Weigh 50g sodium potassium tartrate tetrahydrates to be dissolved in 100ml water, heating is boiled to remove ammonia；Divide and take the water sample after flocculation precipitation pretreatment in right amount, add in 50ml colorimetric cylinders, graticule is diluted to, adds 1.0ml potassium sodium tartrate solutions, divides and takes in right amount through distilling pretreated distillate, add in 50ml colorimetric cylinders, add a certain amount of 1mol/L sodium hydroxide solutions to neutralize boric acid, be diluted to graticule, add 1.5ml nessler reagents, after placing 10min, absorbance is measured；After the absorbance measured by water sample subtracts the absorbance of blank assay, ammonia-nitrogen content m values are calculated with standard curve.The assay method continuous mode is easy, and the numerical value determined is accurate, whether meets safe for drinking according to result judgement water quality, can effectively protect the health of people.
Description
Technical field
The present invention relates to a kind of method, more particularly, to a kind of side of the ammonia-nitrogen content determination efficiency based in increasing water quality
Method.
Background technology
Ammonia nitrogen refers in water with free ammonia（NH3）And ammonium ion（NH4 +）Nitrogen existing for form.Both ratio of components depend on
The pH value and water temperature of water.When pH value is higher, the ratio of free ammonia is higher.Conversely, then the ratio of ammonium salt is high, water temperature then on the contrary,
The nitrogen content of animality organic matter is height typically compared with vegetalitas organic matter.Meanwhile itrogenous organic substance is very unstable in human and animal excreta,
It is readily decomposed to ammonia.Therefore, fixed nitrogen existing in the form of ammonia or ammonium ion is referred to when ammonia-nitrogen content increases in water.Natural terrain water
Mainly with nitrate nitrogen in body and body of groundwater（NO3）Based on, with free ammonia（NH3）And ammonium ion（NH4 +）Nitrogen existing for form
The ammonia nitrogen of polluted water body is hydration ammonia, also referred to as non-ionic ammonia.Non-ionic ammonia is the Main Factors for causing aquatile to be poisoned, and
Ammonium ion is relatively basic nontoxic.The class surface water of national standard III, the mg/litre of the concentration of non-ionic ammonia≤0.02.Ammonia nitrogen is water body
In nutrient, water eutrophication phenomenon can be caused to produce, be the main oxygen consumption pollutant in water body, to fish and some aquatic
Biology is toxic.In the running water that people drink, need to be measured for the ammonia-nitrogen content in water quality, but existing survey
The error that the method for determining determines is big, causes people to be damaged after drinking to body.
The content of the invention
It is an object of the invention to overcome the error of ammonia-nitrogen content in above-mentioned existing assay method measure water quality big, cause people
Drink after the problem of being damaged to body, devise a kind of side of the ammonia-nitrogen content determination efficiency based in increasing water quality
Method, the assay method continuous mode is easy, and the numerical value determined is accurate, and peace is drunk according to whether result judgement water quality meets
Entirely, the health of people can be effectively protected, it is big to solve the error of ammonia-nitrogen content in existing assay method measure water quality, leads
The problem of causing people to be damaged after drinking to body.
The purpose of the present invention is achieved through the following technical solutions：Side based on the ammonia-nitrogen content determination efficiency in increasing water quality
Method, comprise the following steps：
（1）The preparation of reagent：20g KIs are weighed to be dissolved in about 100ml water, it is a small amount of by several times while stirring to add mercuric chloride knot
Crystalline flour end（About 10g）, to when vermilion precipitation occur and not readily dissolving, it is changed to that saturation liquor hydrargyri perchloridi is added dropwise, and is sufficiently stirred,
When the micro vermilion precipitation of appearance not readily dissolves, stop that liquor hydrargyri perchloridi is added dropwise；Separately weighing 60g potassium hydroxide is dissolved in water,
And 250ml is diluted to, after being sufficiently cooled to room temperature, inject slowly in potassium hydroxide solution, use water by above-mentioned solution under agitation
400ml is diluted to, is mixed, is stood, supernatant is moved into polyethylene bottle, close plug preserves stand-by；It is molten to weigh 50g sodium potassium tartrate tetrahydrates
In 100ml water, heating is boiled to remove ammonia, is let cool, constant volume 100ml；3.819g is weighed through 100 DEG C of dried top pure grade chlorine
It is soluble in water to change ammonium, moves into 1000ml volumetric flasks, is diluted to graticule, every milliliter of ammonia nitrogen containing 1.00mg of this solution, pipettes
5.00ml ammoniums Standard Reserving Solution is diluted with water to graticule in 500ml volumetric flasks, every milliliter of ammonia nitrogen containing 0.010mg of this solution；
（2）The making of standard curve：Draw 0,0.50,1.00,3.00,5.00,7.00 and 10.00ml ammoniums standard solution in
In 50ml colorimetric cylinders, graticule is added water to, adds 1.0ml potassium sodium tartrate solutions, shakes up, adds 1.5ml nessler reagents, is mixed, is placed
After 10min, go out in wavelength 420nm, with light path 20mm cuvettes, using water as reference, measure absorbance, subtracted by the absorbance measured
After going the absorbance of blank, obtain correcting absorbance, with ammonia-nitrogen content（mg）Statistical regression standard curve to correcting absorbance；
（3）The measure of water sample：Divide and take the water sample after flocculation precipitation pretreatment in right amount（Ammonia-nitrogen content is set to be no more than 0.1mg）, add
Enter in 50ml colorimetric cylinders, be diluted to graticule, add 1.0ml potassium sodium tartrate solutions, divide and take in right amount through distilling pretreated distillate
Liquid, add in 50ml colorimetric cylinders, add a certain amount of 1mol/L sodium hydroxide solutions to neutralize boric acid, be diluted to graticule, add 1.5ml to receive
Family name's reagent, mix, after placing 10min, according to step（2）Measure absorbance；
（4）As a result calculate：After the absorbance measured by water sample subtracts the absorbance of blank assay, ammonia nitrogen is calculated with standard curve
Content m（mg）Value, is as a result calculated：
Ammonia nitrogen（N, mg/L）=V/m*1000
In formula：M --- the ammonia nitrogen amount checked in by standard curve（mg）；
V --- volume of water sample（ml）.
In summary, the beneficial effects of the invention are as follows：The assay method continuous mode is easy, and it is accurate to determine the numerical value come
Really, whether safe for drinking is met according to result judgement water quality, can effectively protects the health of people, solve existing measure
The error of ammonia-nitrogen content is big in method measure water quality, the problem of causing people to be damaged after drinking to body.
Embodiment
With reference to embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited only to
This.
Embodiment：
Based on the method for the ammonia-nitrogen content determination efficiency in increasing water quality, comprise the following steps：
（1）The preparation of reagent：20g KIs are weighed to be dissolved in about 100ml water, it is a small amount of by several times while stirring to add mercuric chloride knot
Crystalline flour end（About 10g）, to when vermilion precipitation occur and not readily dissolving, it is changed to that saturation liquor hydrargyri perchloridi is added dropwise, and is sufficiently stirred,
When the micro vermilion precipitation of appearance not readily dissolves, stop that liquor hydrargyri perchloridi is added dropwise；Separately weighing 60g potassium hydroxide is dissolved in water,
And 250ml is diluted to, after being sufficiently cooled to room temperature, inject slowly in potassium hydroxide solution, use water by above-mentioned solution under agitation
400ml is diluted to, is mixed, is stood, supernatant is moved into polyethylene bottle, close plug preserves stand-by；It is molten to weigh 50g sodium potassium tartrate tetrahydrates
In 100ml water, heating is boiled to remove ammonia, is let cool, constant volume 100ml；3.819g is weighed through 100 DEG C of dried top pure grade chlorine
It is soluble in water to change ammonium, moves into 1000ml volumetric flasks, is diluted to graticule, every milliliter of ammonia nitrogen containing 1.00mg of this solution, pipettes
5.00ml ammoniums Standard Reserving Solution is diluted with water to graticule in 500ml volumetric flasks, every milliliter of ammonia nitrogen containing 0.010mg of this solution；
（2）The making of standard curve：Draw 0,0.50,1.00,3.00,5.00,7.00 and 10.00ml ammoniums standard solution in
In 50ml colorimetric cylinders, graticule is added water to, adds 1.0ml potassium sodium tartrate solutions, shakes up, adds 1.5ml nessler reagents, is mixed, is placed
After 10min, go out in wavelength 420nm, with light path 20mm cuvettes, using water as reference, measure absorbance, subtracted by the absorbance measured
After going the absorbance of blank, obtain correcting absorbance, with ammonia-nitrogen content（mg）Statistical regression standard curve to correcting absorbance；
（3）The measure of water sample：Divide and take the water sample after flocculation precipitation pretreatment in right amount（Ammonia-nitrogen content is set to be no more than 0.1mg）, add
Enter in 50ml colorimetric cylinders, be diluted to graticule, add 1.0ml potassium sodium tartrate solutions, divide and take in right amount through distilling pretreated distillate
Liquid, add in 50ml colorimetric cylinders, add a certain amount of 1mol/L sodium hydroxide solutions to neutralize boric acid, be diluted to graticule, add 1.5ml to receive
Family name's reagent, mix, after placing 10min, according to step（2）Measure absorbance；
（4）As a result calculate：After the absorbance measured by water sample subtracts the absorbance of blank assay, ammonia nitrogen is calculated with standard curve
Content m（mg）Value, is as a result calculated：
Ammonia nitrogen（N, mg/L）=V/m*1000
In formula：M --- the ammonia nitrogen amount checked in by standard curve（mg）；
V --- volume of water sample（ml）.
The assay method continuous mode is easy, and the numerical value determined is accurate, whether meets drink according to result judgement water quality
With safety, the health of people can be effectively protected, solves the error that existing assay method determines ammonia-nitrogen content in water quality
Greatly, the problem of causing people to be damaged after drinking to body.
It is described above, be only presently preferred embodiments of the present invention, any formal limitation not done to the present invention, it is every according to
Any simply modification, the equivalent variations substantially made according to the technology of the present invention, method to above example, each fall within the present invention
Protection domain within.
Claims (1)
1. the method based on the ammonia-nitrogen content determination efficiency in increasing water quality, it is characterised in that comprise the following steps：
（1）The preparation of reagent：20g KIs are weighed to be dissolved in about 100ml water, it is a small amount of by several times while stirring to add mercuric chloride knot
Crystalline flour end（About 10g）, to when vermilion precipitation occur and not readily dissolving, it is changed to that saturation liquor hydrargyri perchloridi is added dropwise, and is sufficiently stirred,
When the micro vermilion precipitation of appearance not readily dissolves, stop that liquor hydrargyri perchloridi is added dropwise；Separately weighing 60g potassium hydroxide is dissolved in water,
And 250ml is diluted to, after being sufficiently cooled to room temperature, inject slowly in potassium hydroxide solution, use water by above-mentioned solution under agitation
400ml is diluted to, is mixed, is stood, supernatant is moved into polyethylene bottle, close plug preserves stand-by；It is molten to weigh 50g sodium potassium tartrate tetrahydrates
In 100ml water, heating is boiled to remove ammonia, is let cool, constant volume 100ml；3.819g is weighed through 100 DEG C of dried top pure grade chlorine
It is soluble in water to change ammonium, moves into 1000ml volumetric flasks, is diluted to graticule, every milliliter of ammonia nitrogen containing 1.00mg of this solution, pipettes
5.00ml ammoniums Standard Reserving Solution is diluted with water to graticule in 500ml volumetric flasks, every milliliter of ammonia nitrogen containing 0.010mg of this solution；
（2）The making of standard curve：Draw 0,0.50,1.00,3.00,5.00,7.00 and 10.00ml ammoniums standard solution in
In 50ml colorimetric cylinders, graticule is added water to, adds 1.0ml potassium sodium tartrate solutions, shakes up, adds 1.5ml nessler reagents, is mixed, is placed
After 10min, go out in wavelength 420nm, with light path 20mm cuvettes, using water as reference, measure absorbance, subtracted by the absorbance measured
After going the absorbance of blank, obtain correcting absorbance, with ammonia-nitrogen content（mg）Statistical regression standard curve to correcting absorbance；
（3）The measure of water sample：Divide and take the water sample after flocculation precipitation pretreatment in right amount（Ammonia-nitrogen content is set to be no more than 0.1mg）, add
Enter in 50ml colorimetric cylinders, be diluted to graticule, add 1.0ml potassium sodium tartrate solutions, divide and take in right amount through distilling pretreated distillate
Liquid, add in 50ml colorimetric cylinders, add a certain amount of 1mol/L sodium hydroxide solutions to neutralize boric acid, be diluted to graticule, add 1.5ml to receive
Family name's reagent, mix, after placing 10min, according to step（2）Measure absorbance；
（4）As a result calculate：After the absorbance measured by water sample subtracts the absorbance of blank assay, ammonia nitrogen is calculated with standard curve
Content m（mg）Value, is as a result calculated：
Ammonia nitrogen（N, mg/L）=V/m*1000
In formula：M --- the ammonia nitrogen amount checked in by standard curve（mg）；
V --- volume of water sample（ml）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610732225.5A CN107782682A (en) | 2016-08-26 | 2016-08-26 | Method based on the ammonia-nitrogen content determination efficiency in increasing water quality |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610732225.5A CN107782682A (en) | 2016-08-26 | 2016-08-26 | Method based on the ammonia-nitrogen content determination efficiency in increasing water quality |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107782682A true CN107782682A (en) | 2018-03-09 |
Family
ID=61439177
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610732225.5A Withdrawn CN107782682A (en) | 2016-08-26 | 2016-08-26 | Method based on the ammonia-nitrogen content determination efficiency in increasing water quality |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107782682A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108444999A (en) * | 2018-06-20 | 2018-08-24 | 青岛新纪元检测评价有限公司 | The method that Berthelot spectrophotometry measures ammonia-nitrogen content in air |
| CN109142224A (en) * | 2018-10-12 | 2019-01-04 | 山东师范大学 | A kind of intelligent portable ammonia nitrogen detector |
| CN109187388A (en) * | 2018-09-05 | 2019-01-11 | 安徽润安信科检测科技有限公司 | A kind of colorimetry-turbidity compensation measuring method of Liquor-making industry ammonia nitrogen in waste water |
| CN111781198A (en) * | 2020-07-15 | 2020-10-16 | 南通大学 | Two-dimensional determination method for ammonia nitrogen content in water body, soil or sediment |
| CN112964654A (en) * | 2021-01-28 | 2021-06-15 | 上海中西三维药业有限公司 | Method for determining content of ammonia nitrogen in chlorine-containing wastewater and application thereof |
| CN113607725A (en) * | 2021-07-30 | 2021-11-05 | 云南铜业股份有限公司西南铜业分公司 | Method for determining ammonia nitrogen content in wastewater |
-
2016
- 2016-08-26 CN CN201610732225.5A patent/CN107782682A/en not_active Withdrawn
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108444999A (en) * | 2018-06-20 | 2018-08-24 | 青岛新纪元检测评价有限公司 | The method that Berthelot spectrophotometry measures ammonia-nitrogen content in air |
| CN109187388A (en) * | 2018-09-05 | 2019-01-11 | 安徽润安信科检测科技有限公司 | A kind of colorimetry-turbidity compensation measuring method of Liquor-making industry ammonia nitrogen in waste water |
| CN109142224A (en) * | 2018-10-12 | 2019-01-04 | 山东师范大学 | A kind of intelligent portable ammonia nitrogen detector |
| CN111781198A (en) * | 2020-07-15 | 2020-10-16 | 南通大学 | Two-dimensional determination method for ammonia nitrogen content in water body, soil or sediment |
| CN112964654A (en) * | 2021-01-28 | 2021-06-15 | 上海中西三维药业有限公司 | Method for determining content of ammonia nitrogen in chlorine-containing wastewater and application thereof |
| CN113607725A (en) * | 2021-07-30 | 2021-11-05 | 云南铜业股份有限公司西南铜业分公司 | Method for determining ammonia nitrogen content in wastewater |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107782682A (en) | Method based on the ammonia-nitrogen content determination efficiency in increasing water quality | |
| Bundy et al. | Distinct pools of dissolved iron‐binding ligands in the surface and benthic boundary layer of the California Current | |
| Brzezinski et al. | A solvent extraction method for the colorimetric determination of nanomolar concentrations of silicic acid in seawater | |
| CN106525739B (en) | Method for measuring ammonia nitrogen concentration value in water body | |
| CN104034681A (en) | Method for detecting content of ammonia nitrogen in sewage | |
| CN102955015A (en) | Method for determining total nitrogen content in nitrate-nitrogen-containing compound fertilizer through post-distillation titration method | |
| CN107817220B (en) | Method for measuring mass concentration of easily released cyanide in thiocyanate-containing water | |
| CN105424871A (en) | Determination method for ammonia nitrogen in wastewater | |
| CN101101286A (en) | Chlorine dioxide disinfector content detection method | |
| US5415809A (en) | Method for determination of dissolved oxygen in water | |
| Spencer et al. | Azomethine H colorimetric method for determining dissolved boron in water | |
| CN105300980A (en) | Method for rapidly determining soil ammonium nitrogen | |
| Cardiano et al. | Potentiometric, UV and 1H NMR study on the interaction of Cu2+ with ampicillin and amoxicillin in aqueous solution | |
| CN102478525A (en) | Method for determining soil nitrification potential | |
| Lin et al. | Photometric determination of ammonia nitrogen in slaughterhouse wastewater with Nessler's reagent: effects of different pretreatment methods | |
| Kopáčkek et al. | Semi-micro determination of ammonia in water by the rubazoic acid method | |
| US6777242B1 (en) | Aqueous solution based on an azo dye, process for its manufacture and use thereof | |
| JP3997265B2 (en) | Hydrogen peroxide concentration measuring reagent and method for measuring hydrogen peroxide concentration using the same | |
| CN105181410B (en) | The assay method of content of formaldehyde in a kind of N- methylols Methionine calcium salt product | |
| CN107764930A (en) | It is a kind of can in Accurate Determining water quality ammonia-nitrogen content technique | |
| CN113155818B (en) | Reagent for rapidly and quantitatively detecting cyanide, preparation method and detection method thereof | |
| CN107764932A (en) | The method being measured to hardness in drinking water can quickly be realized | |
| CN112964654A (en) | Method for determining content of ammonia nitrogen in chlorine-containing wastewater and application thereof | |
| Sawere et al. | Determination of nitrate and nitrite in surface water in Evwreni town, Delta state | |
| CN104833639A (en) | Detection method of sulfite in food |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180309 |