CN107779493A - ZnO nano particle suppresses the New function of Escherichia coli Growth - Google Patents
ZnO nano particle suppresses the New function of Escherichia coli Growth Download PDFInfo
- Publication number
- CN107779493A CN107779493A CN201711016512.7A CN201711016512A CN107779493A CN 107779493 A CN107779493 A CN 107779493A CN 201711016512 A CN201711016512 A CN 201711016512A CN 107779493 A CN107779493 A CN 107779493A
- Authority
- CN
- China
- Prior art keywords
- nano particle
- escherichia coli
- zno nano
- new function
- zno
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/10—Enterobacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- G01N2333/245—Escherichia (G)
Abstract
Nano particle has the property different from micron above particle, and one of them is embodied in the suppression to bacterium.The application introduces a kind of New function of ZnO nano particle, and major embodiment can be grown with suppression mode microorganism Escherichia coli in the addition of ZnO nano particle and reduce its survival rate, and its mechanism is that have impact on OmpF expression.Because nano particle has become a kind of widely used material, the discovery of the function is advantageous to further recognize its biological effect.
Description
Technical field
The application belongs to the technical field of nano particle New function, suppresses Escherichia coli in particular to ZnO nano particle
The New function of growth.
Background technology
Nano particle is the minitype particle that a kind of made, size is no more than 100 nanometers.Nano material is in science
There is very big value with using upper, in terms of biological effect, nano particle is because the properties such as its diameter is small, surface area is big are past
Toward that can penetrate into theca cell, so as to produce the effect entirely different with micron above particle.ZnO nano particle is a kind of normal
The metal nanoparticle seen, it is a kind of high-end high function fine inorganic product, shows many special properties, such as non-migrating
Property, fluorescence, piezoelectricity etc., be widely used in manufacture gas sensor, rheostat, magnetic material etc..But on
The especially antibacterial property of biology of ZnO nano particle (also known as nano zine oxide) is related to less at present.
Escherichia coli (Escherichia coli) are a kind of common model organisms, are widely used in microbiology, lose
Pass the research of the multiple fields such as, molecular biology.Escherichia coli are equally the common bacterias for carrying out tested material bacteriostasis test
Kind, main method of testing includes growth curve measure, Oxford cup measure etc..The latter on strain growth flat board mainly by pressing down
The presence of bacterium circle and size are measured to the bacteriostasis of tested material.The research of the antagonistic property of nano particle is beneficial to
Its biological effect is specifically illustrated, and is provided fundamental basis for the application in corresponding field.
The content of the invention
Escherichia coli are conventional bacterial strain, purchased from Beijing Ai Bixin Bioisystech Co., Ltd.
ZnO nano particle is purchased from Shanghai Sigma, it is characterized in that diameter be less than 40 nanometers, more than 99% purity, than
Surface area is more than 30 meters squared per grams.
The bacteriostatic activity detection of ZnO nano particle：
The Escherichia coli activated are seeded to fresh culture with 2% and cultivating 4h makes it grow to exponential phase,
Then take 50 microlitres of bacterium solutions to be mixed after diluting 100 times with culture medium and be down flat plate.Oxford cup is inserted simultaneously, is separately added into 150 microlitres
Positive control (ampicillin, 100 nanograms/microlitre), negative control (distilled water), low concentration (20 microgram ZnO nano particle),
Middle concentration (200 microgram ZnO nano particle), the tested material of high concentration (2 milligrams of nano particles), inhibition zone is observed after cultivating 16h
Presence.
Experimental result is as shown in Figure 1.As can be seen from Figure, occur under conditions of high concentration ZnO nano particle disposal
Obvious inhibition zone, this shows that ZnO nano particle has significant fungistatic effect.
Further, carry out repeating above-mentioned Oxford cup bacteriostatic experiment using the concentration as condition, by measuring antibacterial circle diameter,
The data obtained is as shown in table 1.Through statistics, its average diameter is in 12.3mm.
Repeat experiment sequence number antibacterial circle diameter (mm)
| Repeat experiment sequence number | Antibacterial circle diameter (mm) |
| 1 | 10.5 |
| 2 | 14.5 |
| 3 | 12.4 |
| 4 | 10.8 |
| 5 | 13.5 |
Further to explore its antifungal mechanism, add in Escherichia coli liquid culture of the culture to above-mentioned exponential phase
Enter ZnO nano particle, its final concentration is reached 50 mg/mls.Continue after cultivating 16h, reclaim thalline, it is conventional using this area
Technological means extraction cell in total mRNA, reverse transcription cDNA, and use Cycle480 (Roche) quantitative real time PCR Instrument (makes
With SybrGreen dyestuffs) quantitative analysis is carried out to the expression quantity of OmpF genes.Used PCR conditions are：95 DEG C of pre-degenerations
5min, followed by 95 DEG C of 10s, 60 DEG C of 10s, 72 DEG C of 30s amplifications totally 40 circulations.Used primer is：Upstream
CCAGGGTAACAACTCTGAAG；Downstream GCCGCCAACACGACCAACGA.The quantitative value obtained be OmpF expression quantity with
Reference gene GAPDH relative ratio.
The mRNA relative quantification results that experimental group and control group are obtained are as shown in Figure 2.
From figure 2 it can be seen that by the processing of ZnO nano particle, OmpF expression decreases drastically, drop
Low is original 0.65 times.
The application, which demonstrates ZnO nano particle, has significant fungistatic effect, and its potential mechanism is influence outer membrane protein
OmpF expression, this application provide new approaches more completely to recognize the biological effect of metal nanoparticle.
Brief description of the drawings
The Oxford cup bacteriostatic experiment of Fig. 1 ZnO nano particles.1 positive controls；2 negative control groups；3 low concentrations expose；4
Middle concentration exposure；5 high concentrations expose
The expression change of Escherichia coli OmpF genes caused by Fig. 2 ZnO nano particle disposals
Control refers to control group；ZnO ' s addition refer to ZnO nano particle disposal group；Ordinate is relative mRNA water
It is flat.
Embodiment
Embodiment 1
The bacteriostatic activity detection of ZnO nano particle：
The Escherichia coli activated are seeded to fresh culture with 2% and cultivating 4h makes it grow to exponential phase,
Then take 50 microlitres of bacterium solutions to be mixed after diluting 100 times with culture medium and be down flat plate.Oxford cup is inserted simultaneously, is separately added into 150 microlitres
Positive control (ampicillin, 100 nanograms/microlitre), negative control (distilled water), low concentration (20 microgram ZnO nano particle),
Middle concentration (200 microgram ZnO nano particle), the tested material of high concentration (2 milligrams of nano particles), inhibition zone is observed after cultivating 16h
Presence.
Embodiment 2
The influence that ZnO is expressed target gene：
ZnO nano particle is added in Escherichia coli liquid culture of the culture to above-mentioned exponential phase, makes it dense eventually
Degree reaches 50 mg/mls.Continue after cultivating 16h, reclaim thalline, extracted using this area conventional technology total in cell
MRNA, reverse transcription cDNA, and using Cycle480 (Roche) quantitative real time PCR Instrument (using SybrGreen dyestuffs) to OmpF
The expression quantity of gene carries out quantitative analysis.Used PCR conditions are：95 DEG C of pre-degeneration 5min, followed by 95 DEG C of 10s, 60 DEG C
10s, 72 DEG C of 30s amplifications totally 40 circulations.Used primer is：Upstream CCAGGGTAACAACTCTGAAG；Downstream
GCCGCCAACACGACCAACGA.The quantitative value obtained is the relative ratio of OmpF expression quantity and reference gene GAPDH.Through
The processing of ZnO nano particle is crossed, OmpF expression quantity is reduced to 0.65 times of control group.
Claims (4)
1. a kind of nano particle suppresses the New function of Escherichia coli Growth, it is characterised in that nano particle is cultivated in Escherichia coli
Addition on flat board can produce inhibition zone.
2. a kind of New function as claimed in claim 1, it is characterised in that the nano particle is ZnO particle.
3. a kind of New function as claimed in claim 2, it is characterised in that its average diameter of inhibition zone is 12.3 millimeters.
4. a kind of New function as claimed in claim 1, it is characterised in that ZnO nano particle can make in fluid nutrient medium
Escherichia coli OmpF expression quantity is reduced to original 0.65 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711016512.7A CN107779493A (en) | 2017-10-25 | 2017-10-25 | ZnO nano particle suppresses the New function of Escherichia coli Growth |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711016512.7A CN107779493A (en) | 2017-10-25 | 2017-10-25 | ZnO nano particle suppresses the New function of Escherichia coli Growth |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107779493A true CN107779493A (en) | 2018-03-09 |
Family
ID=61435343
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711016512.7A Pending CN107779493A (en) | 2017-10-25 | 2017-10-25 | ZnO nano particle suppresses the New function of Escherichia coli Growth |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107779493A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1568704A (en) * | 2004-04-29 | 2005-01-26 | 天津理工学院 | Composite nanometer antibiotic material, preparation method and products thereof |
| WO2008021441A2 (en) * | 2006-08-15 | 2008-02-21 | Fitchmun Mark I | Surface sanitizer |
| CN101580814A (en) * | 2008-05-15 | 2009-11-18 | 中国医学科学院医药生物技术研究所 | Screening model and application of gram negative bacterium resistant medicament using SecA power pump as target spot |
| CN102417220A (en) * | 2011-11-28 | 2012-04-18 | 南京大学 | Application of tantalum-doped zinc oxide nanometer powder photocatalyst to water treatment antisepsis |
-
2017
- 2017-10-25 CN CN201711016512.7A patent/CN107779493A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1568704A (en) * | 2004-04-29 | 2005-01-26 | 天津理工学院 | Composite nanometer antibiotic material, preparation method and products thereof |
| WO2008021441A2 (en) * | 2006-08-15 | 2008-02-21 | Fitchmun Mark I | Surface sanitizer |
| CN101580814A (en) * | 2008-05-15 | 2009-11-18 | 中国医学科学院医药生物技术研究所 | Screening model and application of gram negative bacterium resistant medicament using SecA power pump as target spot |
| CN102417220A (en) * | 2011-11-28 | 2012-04-18 | 南京大学 | Application of tantalum-doped zinc oxide nanometer powder photocatalyst to water treatment antisepsis |
Non-Patent Citations (3)
| Title |
|---|
| LIU, WENWEN ET AL: "Selenium nanoparticles incorporated into titania nanotubes inhibit bacterial growth and macrophage proliferation", 《NANOSCALE》 * |
| 张凤珍: "大肠杆菌耐药机制和消除耐药性方法概述", 《内蒙古民族大学学报(自然科学版)》 * |
| 邵珍美等: "不同锌源对大肠杆菌体内外抑菌效果的观察", 《养殖技术顾问》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Duran et al. | Removal and inactivation of indicator bacteriophages in fresh waters | |
| Kianpour et al. | Physicochemical and biological characteristics of the nanostructured polysaccharide‐iron hydrogel produced by microorganism Klebsiella oxytoca | |
| Zhen et al. | A systematic comparison of four bioaerosol generators: Affect on culturability and cell membrane integrity when aerosolizing Escherichia coli bacteria | |
| Pinot et al. | Identification of Stenotrophomonas maltophilia strains isolated from environmental and clinical samples: a rapid and efficient procedure | |
| JP5018104B2 (en) | Cell culture method and cell culture apparatus | |
| EP3286330B1 (en) | Particulate distributed sensing elements | |
| Noman et al. | Inactivating pathogenic bacteria in greywater by biosynthesized Cu/Zn nanoparticles from secondary metabolite of Aspergillus iizukae; optimization, mechanism and techno economic analysis | |
| CN102435653A (en) | Field effect transistor-based antibiotic medicine screening device and antibiotic medicine screening method | |
| Xiao et al. | A simple mathematical model for electric cell-substrate impedance sensing with extended applications | |
| Risse-Buhl et al. | Aquatic protists modulate the microbial activity associated with mineral surfaces and leaf litter | |
| CN102586160A (en) | Stenotrophomonas maltophilia DS4 | |
| CN101525583B (en) | Bacillus subtilis dcy-1 and application thereof in biofermentation | |
| Dong et al. | Time-resolved toxicity study reveals the dynamic interactions between uncoated silver nanoparticles and bacteria | |
| Vochita et al. | Magnetic nanoparticle genetic impact on root tip cells of sunflower seedlings | |
| Mohanty et al. | Impact of sublethal levels of single-wall carbon nanotubes on pyoverdine production in Pseudomonas aeruginosa and its environmental implications | |
| CN102583780A (en) | Application of Stenotrophomonas maltophilia DS4 for degrading organic pollutants in saponin waste water | |
| CN107779493A (en) | ZnO nano particle suppresses the New function of Escherichia coli Growth | |
| Kučić Grgić et al. | Screening the efficacy of a microbial consortium of bacteria and fungi isolated from different environmental samples for the degradation of LDPE/TPS films | |
| Răcuciu et al. | Biocompatible magnetic fluid nanoparticles internalized in vegetal tissue | |
| Fang et al. | Transformation of iron in pure culture process of extremely acidophilic microorganisms | |
| CN107771856A (en) | ZnO nano particle suppresses the New function of bacillus subtilis bacteria growing | |
| CN107801733A (en) | Titania nanoparticles suppress the New function of Escherichia coli Growth | |
| Polyanskaya et al. | Sizes of bacterial cells in soils determined by cascade filtration technique | |
| CN110106123A (en) | Citric acid bacterial strain Z3 and its application in reduction of hexavalent chromium ion | |
| NL2033219A (en) | Sulfur quantum dot with high fluorescence quantum yield and mimic oxidase activity, and preparation and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |