CN107761105A - The corrosion inhibiter of steel in a kind of acetic environment of metabolite containing actinomyces - Google Patents
The corrosion inhibiter of steel in a kind of acetic environment of metabolite containing actinomyces Download PDFInfo
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- CN107761105A CN107761105A CN201610697242.XA CN201610697242A CN107761105A CN 107761105 A CN107761105 A CN 107761105A CN 201610697242 A CN201610697242 A CN 201610697242A CN 107761105 A CN107761105 A CN 107761105A
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- corrosion inhibiter
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- C—CHEMISTRY; METALLURGY
- C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
- C23F—NON-MECHANICAL REMOVAL OF METALLIC MATERIAL FROM SURFACE; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL; MULTI-STEP PROCESSES FOR SURFACE TREATMENT OF METALLIC MATERIAL INVOLVING AT LEAST ONE PROCESS PROVIDED FOR IN CLASS C23 AND AT LEAST ONE PROCESS COVERED BY SUBCLASS C21D OR C22F OR CLASS C25
- C23F11/00—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent
- C23F11/08—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent in other liquids
- C23F11/10—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent in other liquids using organic inhibitors
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/06—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using actinomycetales
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
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Abstract
The present invention relates to corrosion inhibiter of steel in a kind of acetic environment of metabolite containing actinomyces and preparation method thereof, the steps such as the corrosion inhibiter preparation method includes the culture of white light gray streptomycete, white light gray streptomycete metabolite is collected and prepared by corrosion inhibiter.Reach more than 83% using the corrosion inhibiter inhibition efficiency obtained by the inventive method.Because from microbial metabolic products, as corrosion inhibiter raw material, raw material sources of the present invention are extensive, easily production, and small toxicity, environmental pollution is small, high financial profit, meets energy-saving and emission-reduction and the requirement of clean manufacturing.
Description
Technical field
The invention belongs to metal inhibitor field.A kind of more particularly it relates to second of metabolite containing actinomyces
The preparation method of the corrosion inhibiter of steel in acid environment.
Background technology
The origin cause of formation of metal erosion be due to surrounding medium neutral ion with material interaction and caused by it is destructive rotten.
Therefore suppress one of metallic material corrosion effective way to be exactly control or change surrounding medium.Corrosion inhibiter need to only be added to corruption on a small quantity
Lose in environment, the condition with regard to environment and medium can be changed.Occur so as to effectively prevent or suppress metallic material corrosion effect.It is slow
Because its equipment is simple, easy to operate, dosage is few, quick, and is widely used in the portions such as machinery, chemical industry, smelting, the energy for agent protection
Door.But it can also be used when the amount of corrosive medium is less.
At present, medicine class corrosion inhibiter is because containing specific functional groups in its main component, and degradable characteristic and be used for
The research of environment-friendly type corrosion inhibiter.Wherein based on antibiotics, due to containing in the molecule of antibiotics main component
There are the groups containing unsaturated bond such as the larger atom of the electronegativity such as O, N, S, P and phenyl ring.Wherein, containing not paired lone electron
Atom can with the interphase interaction of the empty d tracks of metal surface, formed coordinate bond, so as to adsorb in metal surface, rise
To the effect for suppressing corrosion.Also contain a large amount of groups with unsaturated bond in the son of antibiotics main component simultaneously,
These unsaturated bonds can be formed with the stronger big pi bond of electronegativity, and the presence of big pi bond can be the empty d of metal surface atom
Track provides electronics, and then combines to form coordinate bond, corrosion inhibiter is attached to metal surface, slows down corrosion of metal.Therefore, medicine
Species corrosion inhibiter has certain theoretical research foundation.
Actinomyces（Actinomycetes）It is gram-positive bacterium, there is G+C rich, mainly in mycelial growth,
It is bred mainly is carried out in the form of spore, is the stronger prokaryotes of terrestrial, because producing abundant active secondary metabolism production
Thing and it is famous, it is widely distributed in land and marine environment.Wherein, streptomyces microorganism is the leather orchid for belonging to Actinomycetal
Family name's positive bacteria, soil, ocean can be derived from, can also the form of endophyte parasitize in plant.Streptomyces microorganism time
Level metabolite contains antibiotic composition mostly, has good bioactivity and application value.Moreover, streptomyces microorganism
Turn into the important source for finding pilot compound.
In order to solve technological deficiency existing for prior art, the present inventor is on the basis of prior art is summarized, by big
Experimental study and analysis are measured, completes the present invention finally.
The content of the invention
【Technical problem】
It is an object of the invention to provide the preparation method of the corrosion inhibiter of steel in a kind of acetic environment of metabolite containing actinomyces.
【Technical scheme】
The present invention is achieved through the following technical solutions.
The present invention relates to the preparation method of the corrosion inhibiter of steel in a kind of acetic environment of metabolite containing actinomyces, corrosion inhibiter
Main component be white light gray streptomycete secondary metabolite.
The step of preparation method, is as follows：
A, white light gray streptomycete culture
Standard white light gray streptomycete is inoculated on solid medium, is placed in 30 DEG C of constant incubator and carries out activation 1-2 days,
Then
B, white light gray streptomycete metabolite is collected
White light gray streptomycete after the activation obtained toward step B is inoculated into the triangular flask equipped with fluid nutrient medium, by triangular flask
It is placed in the isothermal vibration shaking table that temperature is 30 DEG C, culture seven days, then
C, prepared by corrosion inhibiter
The culture bacterium solution that step B is obtained, centrifuged 20 minutes under 10000 revs/min with centrifuge, remove sediment, take
Clear liquid simultaneously dilutes volumetric concentration to 15% ~ 20% with deionized water, obtains described corrosion inhibiter.
A preferred embodiment of the invention, in step, the solid medium is by 4 grams of glucose, malt
3 grams of sugar, 4 grams of dusty yeast, 20 grams of agar, it is added to heating for dissolving in 1 liter of deionized water and is made.
According to another preferred embodiment of the present invention, in step, constant incubator residing for the solid medium
In temperature be 30 DEG C.
According to another preferred embodiment of the present invention, in step, the solid medium is in constant incubator
Incubation time be -2 days 1 day.
According to another preferred embodiment of the present invention, in stepb, the fluid nutrient medium is by 4 grams of glucose, wheat
3 grams of bud sugar, 4 grams of dusty yeast, it is added to heating for dissolving in 1 liter of deionized water and is made.
According to another preferred embodiment of the present invention, in stepb, constant incubator residing for the fluid nutrient medium
In temperature be 30 DEG C.
According to another preferred embodiment of the present invention, in stepb, the fluid nutrient medium is in constant incubator
Incubation time be 7 days.
According to another preferred embodiment of the present invention, in step C, described corrosion inhibiter is as the training obtained by step B
Bacteria liquid in centrifuge with take after centrifuging 20 minutes under conditions of 10000 revs/min supernatant dilute volumetric concentration to 15% ~
20% gained.
According to another preferred embodiment of the present invention, the inhibition efficiency of the corrosion inhibiter is with IERtMeter up to 82.5% with
On；Up to more than 83% in terms of IE.
【Technique effect】
The beneficial effects of the invention are as follows：Compared with prior art, reached using the corrosion inhibiter inhibition efficiency obtained by the inventive method
To more than 83%.Because from microbial metabolic products, as corrosion inhibiter raw material, raw material sources of the present invention are extensive, easily production, toxicity
Small, environmental pollution is small, high financial profit, meets energy-saving and emission-reduction and the requirement of clean manufacturing.
Brief description of the drawings
Fig. 1 is the surface corrosion pattern for being not added with A3 steel in the acetum of corrosion inhibiter prepared by the inventive method.
Fig. 2 is the surface corrosion pattern of A3 steel in the acetum for the addition of corrosion inhibiter prepared by the inventive method.
Fig. 3 is dynamic potential polarization curve of the A3 steel in the acetate system containing white light gray secondary metabolism of Streptomyces product.
Fig. 4 is electrochemical impedance collection of illustrative plates of the A3 steel in the acetate system containing white light gray secondary metabolism of Streptomyces product.
Embodiment
The present invention relates to the preparation method of the corrosion inhibiter of steel in a kind of acetic environment of metabolite containing actinomyces.
The present invention is prepared using the secondary metabolite of white light gray streptomycete as primary raw material.First, raw material of the present invention
Wide material sources, easily production；Second, small toxicity of the present invention, environmental pollution is small, high financial profit, meets energy-saving and emission-reduction and is given birth to cleaning
The requirement of production.
Below by way of specific embodiment, the invention will be further described, it is noted that following examples are merely to illustrate
The present invention rather than limitation the scope of the present invention.
The white light gray streptomycete culture of embodiment 1
Standard white light gray streptomycete is inoculated on solid medium, is placed in 30 DEG C of constant incubator and carries out activation 1-2 days.
The solid culture based formulas that the present invention uses is as follows：
Glucose4 grams；
Maltose3 grams；
Dusty yeast4 grams；
20 grams of agar；
1 liter of deionized water.
The present invention carries out the culture of white light gray streptomycete using existing constant incubator, such as by PANASONIC strain formula meeting
Society is with trade name electro-heating standing-temperature cultivator（MIR-262-PC）The constant incubator of sale.
In the present invention, used glucose is, for example, by Shanghai Jing Chun biochemical technologies limited company Aladdin board
The glucose of acquisition；Used maltose is, for example, to be obtained by Shanghai Jing Chun biochemical technologies limited company Aladdin board
Maltose；Used dusty yeast is, for example, the yeast obtained by Shanghai Jing Chun biochemical technologies limited company Aladdin board
Powder；Used agar is, for example, the agar obtained by Shanghai Jing Chun biochemical technologies limited company Aladdin board；Used
Deionized water be, for example, the deionized water obtained by Shanghai Jing Chun biochemical technologies limited company Aladdin board.Described examination
Agent can also be commercially available commodity, but their chemical composition should meet above-mentioned requirements.
The white light gray streptomycete metabolite of embodiment 2 is collected
White light gray streptomycete after the activation obtained toward step B is inoculated into the triangular flask equipped with fluid nutrient medium, by triangular flask
It is placed in the isothermal vibration shaking table that temperature is 30 DEG C, cultivates seven days.
The Liquid Culture based formulas that the present invention uses is as follows：
Glucose4 grams；
Maltose3 grams；
Dusty yeast4 grams；
1 liter of deionized water.
The present invention carries out the culture of white light gray streptomycete using existing isothermal vibration shaking table, such as encourages way machinery by Shanghai
Plant engineering Co., Ltd is with trade name isothermal vibration shaking table（FB-110H200Q-C）The isothermal vibration shaking table of sale.
In the present invention, used glucose is, for example, by Shanghai Jing Chun biochemical technologies limited company Aladdin board
The glucose of acquisition；Used maltose is, for example, to be obtained by Shanghai Jing Chun biochemical technologies limited company Aladdin board
Maltose；Used dusty yeast is, for example, the yeast obtained by Shanghai Jing Chun biochemical technologies limited company Aladdin board
Powder；Used deionized water is, for example, the deionization obtained by Shanghai Jing Chun biochemical technologies limited company Aladdin board
Water.Described reagent can also be commercially available commodity, but their chemical composition should meet above-mentioned requirements.
The preparation and performance test of the corrosion inhibiter of embodiment 3
The culture bacterium solution that step B is obtained, centrifuged 20 minutes under 10000 revs/min with centrifuge, remove sediment, take
Clear liquid dilutes volumetric concentration to 15% ~ 20%, obtains described corrosion inhibiter.
The present invention carries out the separation of white light gray secondary metabolism of Streptomyces product using existing centrifuge, such as by Shanghai Lu
Xiang Yi centrifuges Instrument Ltd. is with trade name table model high speed centrifuge（TG16-WS）The centrifuge of sale.
In order to show the corrosion inhibiter performance prepared with white light gray secondary metabolism of Streptomyces product of the present invention, by A3 steel prints
24h plus in the 0.1mol/L acetate systems of the present invention and in the 0.1mol/L acetate systems of the addition present invention is not being soaked respectively,
Temperature is 30 DEG C.As seen from Figure 1, A3 steel surfaces are by heavy corrosion, generate it is a large amount of of different sizes, it is in irregular shape
Corrosion area.There is substantial amounts of loose corrosion product on surface, and forms many corrosion cracks and collapse.This explanation A3 steel is in vinegar
Decay resistance in acid is poor.As seen from Figure 2, A3 steel surfaces are more smooth.Observed by enlarged drawing, Wo Menfa
Present A3 steel surfaces have one layer of fine and close attachment, and the presence of attachment protects A3 steel surfaces not to be corroded.
In order to preferably show that the inhibition of the corrosion inhibiter prepared with white light gray secondary metabolism of Streptomyces product of the present invention is imitated
Fruit, the corrosion inhibition rate of corrosion inhibiter is obtained according to following equation（IE）：
I0, I be respectively be free of and the polarization curve of steel disc during containing corrosion inhibiter on corrosion current.Fig. 3 give A3 steel containing
Dynamic potential polarization curve in the acetate system of white light gray secondary metabolism of Streptomyces product, can obtain sustained release rate and be up to 83%.
In order to preferably show that the inhibition of the corrosion inhibiter prepared with white light gray secondary metabolism of Streptomyces product of the present invention is imitated
Fruit, the corrosion inhibition rate of corrosion inhibiter is obtained according to following equation（IERT）：
Rt, R0Respectively contain and the impedance spectrum of steel disc during without corrosion inhibiter on charge-transfer resistance.Fig. 4 gives A3 steel
Electrochemical impedance collection of illustrative plates in the acetate system containing white light gray secondary metabolism of Streptomyces product, sustained release rate can be obtained and be up to
82.5%。
On the basis of embodiment 3, change the concentration of acetic acid, from 0.1mol/L to 0.5mol/L, all corrosion inhibiter delay
Erosion rate reaches more than 83%.
From embodiment 1-3 result of implementation, prepared by the secondary metabolite of the invention with white light gray streptomycete
Corrosion inhibiter has corrosion mitigating effect to the steel in acetic environment, although existing corrosion inhibiter also has preferable corrosion mitigating effect,
Its production cost is high, unfriendly to environment；And the present invention is not only endangered environment structure, and its raw material sources is extensive, production
Cost is cheap, so as to substantially increase the economic benefit of the corrosion inhibiter of steel in acetic environment.
Claims (2)
1. the corrosion inhibiter of steel in a kind of acetic environment of metabolite containing actinomyces, it is characterised in that the corrosion inhibiter main component
For the secondary metabolite of white light gray streptomycete.
2. according to claim 1, it is characterised in that after described corrosion inhibiter is centrifuged by the culture bacterium solution of white light gray streptomycete
Its supernatant is taken to dilute volumetric concentration to 15% ~ 20% gained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610697242.XA CN107761105A (en) | 2016-08-22 | 2016-08-22 | The corrosion inhibiter of steel in a kind of acetic environment of metabolite containing actinomyces |
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| CN201610697242.XA CN107761105A (en) | 2016-08-22 | 2016-08-22 | The corrosion inhibiter of steel in a kind of acetic environment of metabolite containing actinomyces |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109913384A (en) * | 2018-12-20 | 2019-06-21 | 蚌埠学院 | The preparation method and application of a kind of white light gray streptomycete and its biocontrol agent |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101994122A (en) * | 2009-08-21 | 2011-03-30 | 中国科学院海洋研究所 | Antibiotic carbon steel pickling corrosion inhibitor and application thereof |
| CN103069050A (en) * | 2010-08-20 | 2013-04-24 | 3M创新有限公司 | Rust removing composition |
| CN104480472A (en) * | 2014-12-11 | 2015-04-01 | 中国科学院宁波材料技术与工程研究所 | Biological metal corrosion inhibitor and application thereof |
-
2016
- 2016-08-22 CN CN201610697242.XA patent/CN107761105A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101994122A (en) * | 2009-08-21 | 2011-03-30 | 中国科学院海洋研究所 | Antibiotic carbon steel pickling corrosion inhibitor and application thereof |
| CN103069050A (en) * | 2010-08-20 | 2013-04-24 | 3M创新有限公司 | Rust removing composition |
| CN104480472A (en) * | 2014-12-11 | 2015-04-01 | 中国科学院宁波材料技术与工程研究所 | Biological metal corrosion inhibitor and application thereof |
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| 余晋苗 等: "土霉素在含氯离子的醋酸介质中对冷轧钢的缓蚀作用", 《腐蚀与防护》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109913384A (en) * | 2018-12-20 | 2019-06-21 | 蚌埠学院 | The preparation method and application of a kind of white light gray streptomycete and its biocontrol agent |
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Application publication date: 20180306 |