CN107760791A - The application of gene - Google Patents
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- CN107760791A CN107760791A CN201711048100.1A CN201711048100A CN107760791A CN 107760791 A CN107760791 A CN 107760791A CN 201711048100 A CN201711048100 A CN 201711048100A CN 107760791 A CN107760791 A CN 107760791A
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Abstract
The present invention relates to the application of biology field, more particularly to gene.The invention provides the key gene being closely related in difference expression gene with ovary activation.The key gene of honeybee queen ovary activation provided by the invention has the characteristics of precise positioning；New method is provided for the analysis and research of honeybee queen reproductive trait, while also the follow-up study for the activation of honeybee queen ovary from now on provides method and approach.
Description
Technical field
The present invention relates to the application of biology field, more particularly to gene.
Background technology
China is the first in the world bee-keeping big country, and bee product yield is at the forefront in the world.Meanwhile honeybee is also to maintain ecological environment
The indispensable species of sustainable development, it is plant, the main Pollinating Insect of particularly emerging protected crop.
Honeybee bee colony includes queen bee, worker bee and drone.The reproduction of honeybee and produce offspring by queen bee to complete, queen bee
It is full grown female honeybee, its Major Function is to lay eggs, and other members are the filial generations of queen bee in bee colony；Worker bee is to develop not
Complete female honeybee, they carry all work in bee colony, and under normal circumstances, worker bee is without spawning function；Drone
It is the male member in bee colony, its major function just waits for and virgin king's mating.As can be seen here, can be direct in bee colony
To offspring it is queen bee and drone by gene genetic.But drone with will be die after queen bee mating, it is impossible to reuse.
Therefore, queen bee turns into the breeding stock that can uniquely cultivate and repeatedly utilize.The Major Function of queen bee is spawning, to maintain whole bee colony
Sustainable development.Outstanding queen bee can quickly improve egg production capacity in numerous honeybee season, grow bee colony, largely be produced for nectar flow
Bee product is ready.Queen bee reproductive capacity is improved, swarm breeding speed can be improved, and then accelerate the bee products such as honey, royal jelly
Production, can effectively increase economic efficiency.Therefore, the prolificacy of queen bee is increasingly taken seriously, and improves the numerous of queen bee
Growing power turns into focus.
Ovary is the critical function organ of queen bee reproductive system, is the key for influenceing queen to lay eggs.Ovarian size and development
Degree is not only the important morphological index of bee type level differentiation, and the important symbol of reproduction potentiality.Queen bee has development complete
Ovary, after sexal maturity carry out nuptial flight mating, queen bee all one's life only carry out a nuptial flight mating, afterwards ovary activation, start to produce
Ovum, no longer mated throughout one's life.The ovary of spawning queen bee is morphologically, bigger, heavier than the ovary of virgin.Therefore, ovum
Nest activation is the correlation molecule mark of the precondition that female honeybee embodies fecundity, research and the activation of Screening and Identification honeybee ovary
Remember significant to the honeybee queen for cultivating prolificacy power.
At present, the method that honeybee breeding person mainly uses traditional breed system production of hybrid seeds, the queen bee of high reproductive trait is cultivated.Adopt
Carry out marker assisted selection breeding with molecular labeling, just had begun to early in last century end in the poultry such as pig, ox, sheep kind, in recent years
Come, the full-length genome selection of the poultry kind such as pig, ox is all ongoing like a raging fire.And honeybee breeding is not opened also in terms of molecular breeding
The molecular labeling of maturation is sent, the horizontal breeding of group is even more to lag behind other poultrys kind.At present, researcher's generally use is surveyed
DNA, mRNA molecular labeling of the methods of sequence, chip screening honeybee queen ovary activation, and be a large amount of false positive results, gene
Ovary activation can not be significantly affected.But have no the relevant report of QTL positioning.
The content of the invention
In view of this, the invention provides the application of gene.The present invention is using transcript profile sequencing sequencing to honeybee virgin honeybee
The ovary of king and spawning queen bee carries out transcript profile sequencing, screens the gene of the differential expression in virgin king and spawning king, and passes through
The method that the QTL related to ovarian size is compared, further identification influence the gene of ovary activation.By the above method, obtain
Honeybee queen ovary activation key gene 69.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：
The application of the mark activated the invention provides gene as honeybee queen ovary；The Gene of the gene
Bank numberings are selected from：
In some specific embodiments of the present invention, the expression quantity up-regulation of the gene, the ovary of honeybee queen is sharp
State living；The Gene bank numberings of the gene are selected from：
In some specific embodiments of the present invention, the expression quantity of the gene is lowered, and the ovary of honeybee queen is sharp
State living；The Gene bank numberings of the gene are selected from：
Present invention also offers application of the gene as identification honeybee spawning queen bee and the mark of virgin, the base
The Gene bank numberings of cause are selected from：
In some specific embodiments of the present invention, the expression quantity up-regulation of the gene, the queen bee is spawning queen bee；
The Gene bank numberings of the gene are selected from：
In some specific embodiments of the present invention, the expression quantity of the gene is lowered, and the queen bee is spawning queen bee；
The Gene bank numberings of the gene are selected from：
Present invention also offers a kind of screening technique of the mark of honeybee queen ovary activation, comprise the following steps：
Step 1：The ovary tissue sample of honeybee spawning queen bee and the ovary tissue sample of honeybee virgin are obtained respectively；
Step 2：Extraction obtains the RNA of the ovary tissue sample of the honeybee spawning queen bee and the honeybee virgin honeybee respectively
The RNA of the ovary tissue sample of king；
Step 3：Sequencing obtains the sequence information of the RNA；
Step 4：By sequence alignment, the gene where mRNA sequence information and the mRNA is obtained；
Step 5：It is poor in the ovary tissue of the honeybee spawning queen bee and the ovary tissue of the honeybee virgin to obtain
The gene of different expression, differential expression standard are p<0.05；And obtain the physical location of the gene of the differential expression；
Step 6：The physical location of the gene of the different expression falls in the related QTL sections of ovarian size, as indicates
Thing；
The Gene bank numberings of the mark are selected from：
Present invention also offers a kind of authentication method of honeybee queen ovary state, comprise the following steps：
Step 1：The ovary tissue sample of honeybee spawning queen bee and the ovary tissue sample of honeybee virgin are obtained respectively；
Step 2：Extraction obtains the RNA of the ovary tissue sample of the honeybee spawning queen bee and the honeybee virgin honeybee respectively
The RNA of the ovary tissue sample of king；
Step 3：Sequencing obtains the sequence information of the RNA；
Step 4：By sequence alignment, the gene where mRNA sequence information and the mRNA is obtained；
Step 5：It is poor in the ovary tissue of the honeybee spawning queen bee and the ovary tissue of the honeybee virgin to obtain
The gene of different expression, differential expression standard are p<0.05；And obtain the physical location of the gene of the differential expression；
Step 6：The physical location of the gene of the different expression falls in the related QTL sections of ovarian size, as indicates
Thing；
The Gene bank numberings of the mark are selected from：
Step 7：The expression quantity of mark described in sample to be tested is obtained, if the base of the ovary tissue of the sample to be tested
Because expression quantity variation tendency meets as shown below, the then ovary activation of the sample to be tested：
The present invention applies transcript profile sequencing technologies, and the expression for having grasped mRNA in honeybee queen ovary activation comprehensively becomes
Change situation, and by QTL related to ovarian size comparison, identify in difference expression gene and be closely related with ovary activation
Key gene.The key gene of honeybee queen ovary activation provided by the invention has the characteristics of precise positioning；For honey
The analysis and research of honeybee queen bee reproductive trait provide new method, while are also the follow-up study of the activation of honeybee queen ovary from now on
Provide method and approach.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the required accompanying drawing used in technology description to be briefly described.
Fig. 1 shows ovary activation differential expression mRNA GO enrichment results Top 20；
Fig. 2 shows ovary activation differential expression mRNAK EGG pathway results Top 20.
Embodiment
The invention discloses the application of gene, those skilled in the art can use for reference present disclosure, be suitably modified technique ginseng
Number is realized.In particular, all similar replacements and change are apparent to those skilled in the art,
They are considered as being included in the present invention.The method of the present invention and application are described by preferred embodiment, related
Personnel can substantially not depart from present invention, method described herein and application are being modified in spirit and scope or suitably
Change is with combining, to realize and using the technology of the present invention.
Raw materials used and reagent can be bought by market in the application of gene provided by the invention.
With reference to embodiment, the present invention is expanded on further：
Embodiment 1
Sample collection：
Experimental bees queen bee is provided by China Agriculture Industitute Bee Research Center Hainan experiment bee farm.Select 2 groups (every group 3
Individual repetition) health, the basically identical Italian virgin of body condition.Wherein, the 1st group gathers ovary in virgin king's sexal maturity period
Organize (virgin king's group)；Other 1 group of carry out artificial insemination processing, ovary tissue is gathered after queen bee normal spawning.Institute is in a organized way
It is put into liquid nitrogen rapidly and freezes after sample collection, the extraction for RNA.
Tissue sample Total RNAs extraction：
(1) 1mlTrizol (Invitrogen) will be added in preprepared 1.5ml centrifuge tubes, writes sample number into spectrum.
(2) sample for needing to extract RNA is taken out from liquid nitrogen, liquid nitrogen is added in mortar and is ground.Remain
Sample is present in liquid nitrogen, grinds more thin better.
(3) sample for being ground into powdery is poured into pre-prepd Trizol, jiggles greatly to sample and be dissolved completely in
It is extremely transparent in Trizol.
(4) 4 DEG C, 12000rpm, centrifuge 10-15min.
(5) supernatant is taken, adds 0.2ml chloroforms, acutely mixes 15s, places 10min on ice.
(6) 4 DEG C, 12000rpm, centrifuge 10-15min.
(7) supernatant is taken, adds 0.5ml isopropanols, it is light to shake to precipitation precipitation.
(8) 10min on ice is placed.
(9) 4 DEG C, 12000rpm, centrifuge 10-15min.
(10) supernatant is removed, remaining liquid is washed off with pipette tips.
(11) 1ml 75% ethanol, washing precipitation are added.
(12) 4 DEG C, 12000rpm, centrifuge 10-15min.
(13) it is repeated once washing process.
(14) 4 DEG C, 12000rpm, centrifuge 10-15min.
(15) liquid is poured out, washes remaining liq off, room temperature is dried, about 3min.
(16) about 60-100 μ l DEPC water dissolving RNA is added, 15min is dissolved in placement on ice.
(17) -80 DEG C of refrigerators are stored in.
MRNA sequencing libraries are built：
(1) ribose is removed using Epicentre Ribo-zeroTM rRNA Removel Kit (Epicentre, USA)
Body RNA (rRNA)；
(2) useUltraTM Directional RNA Library Prep Kit for
(NEB, USA) builds sequencing library.
(3) microarray dataset Illumina Hiseq 4000, sequence 150bp paired-end reads are produced.
(4) reference gene group Amel_4.5.
MRNA identification：
Raw reads obtain clean reads after Quality Control.Using TopHat v2.0.9 softwares, by clean
Reads carries out mapping with honeybee genome, and according to the position of known mRNA and lncRNA in genome, identification obtains
The mRNA and lncRNA known.
Difference mRNA screening：
Sequencing data is analyzed using DESeq package (1.8.3) program bag, difference between any two groups of screening
The mRNA of expression.Difference test P values are calculated using Benjamini＆Hochber.Differential expression screening standard is correction P<0.05,
FC>1。
Embodiment 2
Real-time quantitative PCR is verified：
The chains of cDNA first synthesize
Using with sequencing used in identical RNA sample carry out cDNA synthesis, use the following steps carry out cDNA synthesis.
(1) following components is added in the microcentrifugal tube of nuclease free.
(2) mixture is immediately placed in cooled on ice after 65 DEG C are heated 5min.After of short duration centrifugation, following components is added：
| 5 × the first chains synthesize buffer solution | 4μl |
| 0.1M DTT | 2μl |
| RNaseOUTTM Nucleic acid inhibitors (40 units/μ l) | 1μl |
(3) gently various composition is mixed in centrifuge tube, and 2min is incubated at 37 DEG C.
(4) 1 μ l (200 unit) M-MLV reverse transcriptases are added at room temperature, and gently piping and druming mixes.If using with power traction
Thing at 25 DEG C by centrifuge tube, it is necessary to be incubated 10mim.
(5) it is incubated 50min 37.
(6) 15min terminating reactions are heated at 70 DEG C.
The cDNA reaction solutions of synthesis can be positioned over -20 DEG C of preservations.
Fluorescence quantification PCR primer designs：
Gene order derives from sequencing result, and using the software Design primers of Oligo 6.0, primer is by Shanghai English fine horse biology skill
Art Co., Ltd synthesizes, and primer sequence is shown in Table 1.From actin as reference gene.
The real-time quantitative PCR primer sequence of table 1
Quantitative fluorescent PCR reaction system and program：
Using the cDNA of above-mentioned synthesis as template, reaction system of the cumulative volume for 20 μ l is used, adds following component：
| SYBR Green I Master Mix | 10μl |
| Water,PCR-grade | 3μl |
| Primer | Each 0.2 μM of upstream and downstream |
| cDNA | 5μl(50ng,1:100dilution) |
| Cumulative volume | 20μl |
Quantitative fluorescent PCR response procedures are：95 DEG C of 10min of pre-degeneration；Amplification setting 45 circulates and collects fluorescence signal：
95 DEG C of denaturation 10s, 60 DEG C of annealing 10s, 72 DEG C of extension 10s；65 DEG C -97 DEG C, 0.2 DEG C/s generation solubility curves；40 DEG C of coolings are eventually
Only react.
The acquisition of RNA relative expression quantities：
Using comparing Ct methods (2-△△Ct) carry out gene mRNA relative expression quantity statistical analysis.Compare what CT methods used
On condition that require that target gene must be consistent with the amplification efficiency of housekeeping gene.This experiment is used as housekeeping gene using actin and U6
To correct the difference of different sample original templates.Tested in triplicate per secondary response, obtain the individual Average Ct values, and use
2-△△CtMethod obtains the RNA relative expression quantities of sample target gene.2-△△CtFormula used in method is as follows：△ △ Ct=[Ct (purposes
Gene, unknown sample)-Ct (housekeeping gene, unknown sample)]-[Ct (target gene, unknown sample)-Ct (housekeeping gene, it is unknown
Sample)].The 2 of each sample-△△CtValue is sample target gene mRNA relative expression quantity.
Real-time quantitative PCR is verified：
It has selected 5 mRNA and carried out real-time quantitative PCR checking.The packet of test specimen is consistent with sequencing part, runs one's home
Reference genes of the gene actin as mRNA.The calculating of expression quantity uses 2-ΔΔCtMethod, sequencing result are shown in compared with quantitative result
Table 2.
As a result find, the expression trend of quantitative testing result and sequencing assay result is basically identical, illustrates that sequencing result is true
It is real reliable.
The mRNA of table 2 quantitative PCR checking
The differential expression mRNA of embodiment 3 enrichment analysis
Ovary activates and laid eggs the function enrichment analysis of differential expression mRNA in regulation process：
GO enrichment analyses：
By the analysis to chip of expression spectrum data, the present invention has obtained multigroup difference RNAs, in order to understand these differences
The biological function of gene and the biological process that they are participated in, function enrichment analysis is carried out to the differential gene of acquisition.Work(
Analysis, which can be enriched with, helps us to further appreciate that difference RNAs information and the biological process that they are participated in from biology angle.
Gene Ontology (GO) analyses can be by gene according to molecular function (GO_MF), cell composition (GO_CC) and bioprocess
(GO_BP) three bodies are classified, and each body can continue to separate different subclass downwards again, form one downwards layer by layer
Body arborizations structure.Now list the GO_BP results (P of significant enrichment<0.05).
In ovary activation, the GO_BP processes of difference mRNA significant enrichments include tissue development, energy production and swashed
(see Fig. 1) such as the anabolism of element.
KEGG pathway enrichment analyses：
After the homologous genes for drosophila of mRNA of the differential expression of acquisition, enrichment analysis is carried out, to can be more fully
Understand lncRNA biological function.The genome of drosophila downloads (Release 74) from Ensembl databases.Homologous alignment parameters
For "-p blastx-m8-e 1e-5-F F ", alignment peptides>50.GO enrichment analyses use Goseq based
Wallenius non-central hyper-genomertic distribution(Young et al.2012)。KEGG
Pathways analyses use KOBAS software (Mao et al.2005).
The GO terms of difference RNAs significant enrichments are analyzed, in addition, many RNAs pass through in vivo
Participate in biological pathway and play its function.Regulate and control to further appreciate that we obtain these differences mRNAs enrichments
Which biological pathways, we have carried out Pathway enrichment analyses to difference mRNAs.Pathway analysis be will participate in it is same
The gene of bioprocess is concentrated in a path and studied.
Ovary is activated and the mRNA of the differential expression for regulation process of laying eggs has carried out pathway enrichment analyses, is as a result seen
Fig. 2.By analyzing the pathway being enriched to, it is found that include inositol in the biological pathways of ovary activation significant enrichment
Six phospholipid metabolisms, glycerophosphatide metabolism, hippo signal paths, phosphoinositide metabolism, MAPK signal paths, neural activity part-
Acceptor interaction, notch signal paths, phospholipid inositol signal system and Wnt signal paths.
The mRNA QTLs related to ovarian size of the differential expression of embodiment 4 comparison
MRNA QTLs related to ovarian size comparison：
The mRNA for the differential expression that the present invention obtains physical location is navigated on honeybee genome, and by mRNA physics
Position is compared with the QTL sections related with honeybee ovarian size being currently known.The QTL deciding fields are in No. 11 chromosomes
Between 8.9Mb to 12.2Mb.Fall the gene and lncRNA in the QTL sections, as influence honeybee ovarian size and spawning
Candidate gene.
Honeybee ovary mRNA expressions:
MRNA sequencing results are shown, in ovary activation, 3218 mRNA up-regulated expressions, under 2263 mRNA expression
Adjust.Partial results are shown in Table 3.
The ovary activation differential expression mRNA top30 lists of table 3
NA " is inapplicable, because denominator is 0 when calculating, can not be calculated.Such case, it is considered to be significant difference is expressed
's.
When the mRNA of differential expression physical location falls in the related QTL confidential intervals of ovarian size, these are obtained
MRNA is ovarian size and the candidate molecular marker gene of spawning.By this method, we identify 73 candidate gene (tables
4)。
Table 4 falls the gene and lncRNA in ovarian size QTL sections
Key gene and expression in the queen bee ovary activation of table 5
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Sequence table
<110>China Agriculture Industitute Bee Research Center
<120>The application of gene
<130> MP1715151
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agacgaacat cagctccagt 20
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<211> 20
<212> DNA
<213> Artificial Sequence
<400> 2
tatagcgaca cctccagcag 20
<210> 3
<211> 22
<212> DNA
<213> Artificial Sequence
<400> 3
tgcctcaatt tgtacaatgg gt 22
<210> 4
<211> 20
<212> DNA
<213> Artificial Sequence
<400> 4
acacayyccy acaccaccca 20
<210> 5
<211> 20
<212> DNA
<213> Artificial Sequence
<400> 5
cgaccttggt ctctggaact 20
<210> 6
<211> 21
<212> DNA
<213> Artificial Sequence
<400> 6
cccaatggag aactgaatgg g 21
<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence
<400> 7
aggcagccag aacattaggt 20
<210> 8
<211> 20
<212> DNA
<213> Artificial Sequence
<400> 8
ttacccgcct ttctaccgag 20
<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence
<400> 9
cctggaactg ttgctcttcg 20
<210> 10
<211> 20
<212> DNA
<213> Artificial Sequence
<400> 10
cttgcgcaat ttcacgaacg 20
<210> 11
<211> 20
<212> DNA
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<400> 11
ctgctgcatc atcctcaagc 20
<210> 12
<211> 20
<212> DNA
<213> Artificial Sequence
<400> 12
gaaaagagcc tcgggacaac 20
Claims (8)
1. the application for the mark that gene activates as honeybee queen ovary；The Gene bank numberings of the gene are selected from：
2. application according to claim 1, it is characterised in that the expression quantity up-regulation of the gene, the ovary of honeybee queen
For state of activation；The Gene bank numberings of the gene are selected from：
3. application according to claim 1, it is characterised in that the expression quantity of the gene is lowered, the ovary of honeybee queen
For state of activation；The Gene bank numberings of the gene are selected from：
4. application of the gene as identification honeybee spawning queen bee and the mark of virgin, it is characterised in that the gene
Gene bank numberings are selected from：
5. application according to claim 4, it is characterised in that the expression quantity up-regulation of the gene, the queen bee are spawning
Queen bee；The Gene bank numberings of the gene are selected from：
6. application according to claim 4, it is characterised in that the expression quantity of the gene is lowered, and the queen bee is spawning
Queen bee；The Gene bank numberings of the gene are selected from：
7. a kind of screening technique of the mark of honeybee queen ovary activation, it is characterised in that comprise the following steps：
Step 1：The ovary tissue sample of honeybee spawning queen bee and the ovary tissue sample of honeybee virgin are obtained respectively；
Step 2：Extraction obtains the RNA of the ovary tissue sample of honeybee spawning queen bee and the honeybee virgin respectively
The RNA of ovary tissue sample；
Step 3：Sequencing obtains the sequence information of the RNA；
Step 4：By sequence alignment, the gene where mRNA sequence information and the mRNA is obtained；
Step 5：Obtain difference table in the ovary tissue of the honeybee spawning queen bee and the ovary tissue of the honeybee virgin
The gene reached, differential expression standard are p<0.05；And obtain the physical location of the gene of the differential expression；
Step 6：The physical location of the gene of the different expression falls in ovarian size QTL sections, as mark；
The Gene bank numberings of the mark are selected from：
8. a kind of authentication method of honeybee queen ovary state, it is characterised in that comprise the following steps：
Step 1：The ovary tissue sample of honeybee spawning queen bee and the ovary tissue sample of honeybee virgin are obtained respectively；
Step 2：Extraction obtains the RNA of the ovary tissue sample of honeybee spawning queen bee and the honeybee virgin respectively
The RNA of ovary tissue sample；
Step 3：Sequencing obtains the sequence information of the RNA；
Step 4：By sequence alignment, the gene where mRNA sequence information and the mRNA is obtained；
Step 5：Obtain difference table in the ovary tissue of the honeybee spawning queen bee and the ovary tissue of the honeybee virgin
The gene reached, differential expression standard are p<0.05；And obtain the physical location of the gene of the differential expression；
Step 6：The physical location of the gene of the different expression falls in ovarian size QTL sections, as mark；
The Gene bank numberings of the mark are selected from：
Step 7：The expression quantity of mark described in sample to be tested is obtained, if the gene table of the ovary tissue of the sample to be tested
Meet as shown below, the then ovary activation of the sample to be tested up to amount variation tendency：
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| CN109628612A (en) * | 2019-01-14 | 2019-04-16 | 中国农业科学院蜜蜂研究所 | The application of lncRNA |
| CN110846421A (en) * | 2019-12-03 | 2020-02-28 | 中国农业科学院蜜蜂研究所 | Application of gene and detection kit thereof |
| CN112365920A (en) * | 2020-09-30 | 2021-02-12 | 中国农业科学院蜜蜂研究所 | Method for identifying bee differentiation key gene, gene obtained by identification and application |
| CN115181745A (en) * | 2022-04-01 | 2022-10-14 | 中国农业科学院蜜蜂研究所 | siRNA for inhibiting honeybee Rho1 gene and application thereof |
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| CN105504037A (en) * | 2016-01-27 | 2016-04-20 | 中国农业科学院蜜蜂研究所 | Gene and application thereof |
| CN105525027A (en) * | 2016-02-22 | 2016-04-27 | 中国农业科学院蜜蜂研究所 | SNP marker as well as application and detection method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109628612A (en) * | 2019-01-14 | 2019-04-16 | 中国农业科学院蜜蜂研究所 | The application of lncRNA |
| CN110846421A (en) * | 2019-12-03 | 2020-02-28 | 中国农业科学院蜜蜂研究所 | Application of gene and detection kit thereof |
| CN112365920A (en) * | 2020-09-30 | 2021-02-12 | 中国农业科学院蜜蜂研究所 | Method for identifying bee differentiation key gene, gene obtained by identification and application |
| CN112365920B (en) * | 2020-09-30 | 2024-04-02 | 中国农业科学院蜜蜂研究所 | Method for identifying bee differentiation key genes, identified genes and application |
| CN115181745A (en) * | 2022-04-01 | 2022-10-14 | 中国农业科学院蜜蜂研究所 | siRNA for inhibiting honeybee Rho1 gene and application thereof |
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