CN107760730A - One Plant Hormone and its application - Google Patents
One Plant Hormone and its application Download PDFInfo
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- CN107760730A CN107760730A CN201711212717.2A CN201711212717A CN107760730A CN 107760730 A CN107760730 A CN 107760730A CN 201711212717 A CN201711212717 A CN 201711212717A CN 107760730 A CN107760730 A CN 107760730A
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- China
- Prior art keywords
- gibberellin
- plant hormone
- phaeodactylum tricornutum
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- phytohormone
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Organic Chemistry (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to technical field of microalga biology, specifically a Plant Hormone and its application.Plant hormone is gibberellin.State application of the phytohormone gibberellin in promoting Phaeodactylum tricornutum to improve grease yield.Gibberellin of the present invention can improve the photosynthetic rate of Phaeodactylum tricornutum and promote its growth, significantly improve the neutral fats content of Phaeodactylum tricornutum.The present invention is simple and easy, cost is cheap, and object fat content is high, can be used as industrial biological oil-producing raw material, be had broad application prospects in terms of biodiesel exploitation.
Description
Technical field
The invention belongs to technical field of microalga biology, specifically a Plant Hormone and its application.
Background technology
With the rapid development of China's economy, the consumption of the energy is also increasing year by year, has already consumed by substantial amounts of energy at present
Source, and these fossil energies cause serious harm to environment during consuming, thus find reproducible clean energy resource
It is extremely urgent.In recent years, alternative fuel of the biodiesel as fossil energy, it has also become with fastest developing speed in the world, application
Most wide environmentally friendly regenerative resource.Microalgae has the advantages that growth rate is fast, can accumulate a large amount of lipids rapidly, it has also become Ren Menyan
Study carefully the preferred material of biodiesel.Micro algae growth does not need soil, can be grown in open pond, industrial land and photo-biological
Reactor etc., waste water and nutrient recycling can also be utilized to combine culture.Thus microalgae is considered as a new generation, even unique
The biodiesel raw material for substituting petrifaction diesel completely can be realized.Phaeodactylum tricornutum is Bacillariophyta, plumage decorative embellishments of writing guiding principle, the one of brown algae category
The unicellular swim alga kind of kind, lipid content is high, is the excellent algae kind of microalgae biodiesel exploitation.
Study hotspot is to probe into the method for improving microalgae grease content at present, but current for inducing microalgae Lipid-producing
Method is mostly two-steps tissue culture method, and this process needs substantial amounts of manpower and materials, and big city suppresses the growth of algae, and is had
The shortcomings that process is complicated, cost is high.Therefore, a kind of new sustainable abductive approach is found, turns into scientist's focus of attention.
Plant hormone has the material of physiology and biological effect, has the function of regulating plant growth and development.As the non-of external source
Trophism chemical substance, plant hormone can generally conduct to site of action in plant, with very low concentration just can promote or
Suppress some links of its life process, be allowed to develop to the needs for meeting the mankind.At present, plant hormone has been successfully applied to
Higher plant Lipid-producing is induced, and its biomass can be improved, therefore, plant hormone is used for reference and contains applied to microalgae grease is improved
Amount.Gibberellin is a Plant Hormone, and the most prominent effect of gibberellin is to accelerate the elongation of cell (gibberellin can improve plant
The content of tumor growth element, and auxin directly adjusts the elongation of cell), the division to cell also has facilitation, and it can be with
Promote the expansion (but not causing the acidifying of cell membrane) of cell, in addition, gibberellin, which also has, suppresses ripe, lateral bud dormancy,
Aging, the physiological action of tuber character.
The content of the invention
It is an object of the invention to provide a Plant Hormone and its application.
To achieve the above object, the present invention use technical scheme for：
One Plant Hormone, plant hormone are gibberellin.
Application of the phytohormone gibberellin in promoting Phaeodactylum tricornutum to improve grease yield.
The phytohormone gibberellin addition is 0.01-20mg/L.The phytohormone gibberellin addition time is phaeodactylum tricornutum
Refer to algae culture to stationary phase (OD730For 0.12-0.15).
A kind of application of gibberellin in as plant hormone.
Application of the gibberellin in promoting Phaeodactylum tricornutum to improve grease yield as plant hormone.
A kind of method for improving Phaeodactylum tricornutum fat content, by gibberellin addition in culture to exponential phase later stage
Phaeodactylum tricornutum algae solution (OD730For 0.12-0.15) in, in 23-25 DEG C, light intensity be 30-35 μm of oLm-2·s-1, Light To Dark Ratio be
12h/12h, incubation time are 6 days.
The gibberellin addition is 0.01-20mg/L.
The phytohormone gibberellin addition is 0.15-1.5mg/L.
Compared with prior art, its advantage is the present invention：
The inventive method is safe and pollution-free, simple, speed is fast, cost is low, is capable of the induction Phaeodactylum tricornutum of stability and high efficiency
Rapid Accumulation grease
1. the present invention can be improved the photosynthetic rate of Phaeodactylum tricornutum and be promoted its growth using gibberellin, three are significantly improved
The neutral fats content of angle brown algae.
2. the present invention is simple and easy, cost is low, pollution-free, stability and high efficiency.
3. one-step method improves fat content, and can improve biomass, can be as the method for industrial biological oil-producing.
Brief description of the drawings
Fig. 1 is to be cultivated in Phaeodactylum tricornutum provided in an embodiment of the present invention after the phytohormone gibberellin of addition various concentrations
The growth curve of six days compares figure；
Fig. 2 is to be cultivated in Phaeodactylum tricornutum provided in an embodiment of the present invention after the phytohormone gibberellin of addition various concentrations
The photosynthetic rate of six days compares figure；
Fig. 3 is to be cultivated in Phaeodactylum tricornutum provided in an embodiment of the present invention after the phytohormone gibberellin of addition various concentrations
The neutral fats relative amount of six days compares figure；
Embodiment
Below in conjunction with drawings and examples, the invention will be further described.
Embodiment 1：
Phytohormone gibberellin induces Phaeodactylum tricornutum
Step 1, prepare algae solution：In 30-35 μm of 23-25 DEG C of temperature, light intensity oLm-2·s-1, Light To Dark Ratio 12h/12h conditions
Under, culture Phaeodactylum tricornutum to exponential phase obtains algae solution close to stationary phase (OD730 0.12-0.15)；
Step 2, hormone induction：Gibberellin is added in the 300ml algae solutions that step 1 obtains, until final concentration of
The control group of gibberellin is not added in 0.15mg/L, 1.5mg/L, 2mg/L, 20mg/L, setting；
Step 3, growth of Phaeodactylum tricornutum curve：By the algae solution after step 2 hormone induction in 23-25 DEG C of temperature, light intensity 30-
35μmoL·m-2·s-1, cultivate 6 days under the conditions of Light To Dark Ratio 12h/12h.
1) measure of phytohormone gibberellin induction growth of Phaeodactylum tricornutum curve：
In the Phaeodactylum tricornutum algae solution that embodiment 1 adds hormone induction culture 0-6 days, 200 μ L are taken daily in ELIASA
Middle measure OD730 (referring to Fig. 1).
Test data：The algae solution absorbance OD730 that the present invention obtains draws induction Vee formation brown algae growth curve (figure
1).To prove the effect of the present invention, to set the blank control group for not adding gibberellin；During gibberellin inducement, phaeodactylum tricornutum refers to
For algae growth curve compared with blank control group, growth is not suppressed.
2) measure of phytohormone gibberellin induction Phaeodactylum tricornutum photosynthetic rate
The Phaeodactylum tricornutum of hormone induction culture 0-6 days is added in embodiment 1, under 0 day, 3 days, 6 days various concentrations
Algae solution application modulated chlorophyll fluorescence instrument (PAM) measurement Y (II) value (i.e. actual photosynthesis efficiency) (referring to Fig. 2).
Test data：The Phaeodactylum tricornutum photosynthetic rate of acquisition draws (Fig. 2).To prove the effect of the present invention, with setting
Not plus gibberellin blank control group.During the gibberellin inducement of various concentrations, Phaeodactylum tricornutum photosynthetic rate and blank pair
According to group compared to not suppressed.
3) measure of phytohormone gibberellin induction Phaeodactylum tricornutum neutral fats content
Take 1mL to add the Phaeodactylum tricornutum of hormone induction culture 0-6 days in embodiment 1, take 0 day, 3 days, 6 days differences respectively
Algae solution under concentration, 2mL distilled water diluting is separately added into algae solution, surveys OD730.Take 300uL to dilute algae solution, add 150uL
DMSO, 2.55mL distilled water is added after mixing.The μ g/mL Nile red acetone solns of 30uL 1 are added, 45s is placed in darkroom after mixing.
Excitation wavelength 527nm, launch wavelength 569nm survey fluorescence angle value (referring to Fig. 3).Neutral fats relative amount calculation formula：
Test data：Neutral fats relative amount draws (Fig. 3).To prove the effect of the present invention, do not add gibberellin to set
Blank control group.0.15mg/L, 1.5mg/L, 2mg/L, 20mg/L gibberellin inducement are added after 6 days, Phaeodactylum tricornutum is neutral
Fat relative amount is respectively 446.82,399.76,264.92,262.48, and control group neutral fats relative amount is 358.14.Addition
Phaeodactylum tricornutum neutral fats content of the 0.15mg/L gibberellin inducements after 6 days improves 50.28%, and neutral fats content highest and
Higher than control group.
Claims (8)
- A 1. Plant Hormone, it is characterised in that：Plant hormone is gibberellin.
- 2. the application of the plant hormone as described in claim 1, it is characterised in that：The phytohormone gibberellin is promoting triangle Brown algae improves the application in grease yield.
- 3. the application of the plant hormone as described in claim 2, it is characterised in that：The phytohormone gibberellin addition is 0.01-20mg/L。
- A kind of 4. application of gibberellin in as plant hormone.
- 5. the gibberellin as described in claim 4 is in the application as plant hormone, it is characterised in that：The gibberellin is in conduct Plant hormone promotes Phaeodactylum tricornutum to improve the application in grease yield.
- A kind of 6. method for improving Phaeodactylum tricornutum fat content, it is characterised in that：Gibberellin addition is given birth in culture to logarithm In the Phaeodactylum tricornutum algae solution in long-term later stage, in 23-25 DEG C, light intensity be 30-35 μm of oLm-2·s-1, Light To Dark Ratio 12h/ 12h, incubation time are 6 days.
- 7. the method for carrying 1 high Phaeodactylum tricornutum fat content as described in claim 6, it is characterised in that：The gibberellin adds Dosage is 0.01-20mg/L.
- 8. the method for the raising Phaeodactylum tricornutum fat content as described in claim 7, it is characterised in that：The plant hormone is red Mycin addition is 0.15-1.5mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711212717.2A CN107760730A (en) | 2017-11-28 | 2017-11-28 | One Plant Hormone and its application |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711212717.2A CN107760730A (en) | 2017-11-28 | 2017-11-28 | One Plant Hormone and its application |
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| CN107760730A true CN107760730A (en) | 2018-03-06 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120034698A1 (en) * | 2009-04-02 | 2012-02-09 | Rosetta Green Ltd. | Compositions and methods for increasing oil content in algae |
| CN106190848A (en) * | 2016-06-23 | 2016-12-07 | 临沂大学 | A kind of waste and old easy open can and the method for narrow-mouthed bottle two stage culture Phaeodactylum |
| CN107384802A (en) * | 2017-08-23 | 2017-11-24 | 山东大学 | A kind of method for promoting microalgae grease to accumulate and keep microalgae high-biomass |
-
2017
- 2017-11-28 CN CN201711212717.2A patent/CN107760730A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120034698A1 (en) * | 2009-04-02 | 2012-02-09 | Rosetta Green Ltd. | Compositions and methods for increasing oil content in algae |
| CN106190848A (en) * | 2016-06-23 | 2016-12-07 | 临沂大学 | A kind of waste and old easy open can and the method for narrow-mouthed bottle two stage culture Phaeodactylum |
| CN107384802A (en) * | 2017-08-23 | 2017-11-24 | 山东大学 | A kind of method for promoting microalgae grease to accumulate and keep microalgae high-biomass |
Non-Patent Citations (1)
| Title |
|---|
| 范新照等: "植物生长调节剂2,4-D和GA_3对微茫藻18A8(Micractinium sp.)生长和油脂积累的影响", 《热带农业科学》 * |
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Application publication date: 20180306 |