CN107760633A - The solid medium of streptococcus pneumonia - Google Patents
The solid medium of streptococcus pneumonia Download PDFInfo
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- CN107760633A CN107760633A CN201711235436.9A CN201711235436A CN107760633A CN 107760633 A CN107760633 A CN 107760633A CN 201711235436 A CN201711235436 A CN 201711235436A CN 107760633 A CN107760633 A CN 107760633A
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- solid medium
- soya peptone
- yeast extract
- streptococcus pneumonia
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses the solid medium of streptococcus pneumonia, the formula composition of the solid medium is that solvent is that the relative usage of water is 1000ml：It is trypticase containing nitrogen source：20~85g, tryptone：25~80g, selected soya peptone：15~70g, soya peptone：20~85g, acid hydrolyzed casein：15~75g, yeast extract：15~35g, yeast extract：Wherein three kinds of 10~45g；Carbon source is glucose：15~55g；Salt is K2HPO4：3.0~15.0g, NaCl：2.0~10.0g, MgSO4·7H2O：0.1~4.0g, CaCl2：0.10~0.18g；Trace element is L cysteine hydrochlorides：0.12~1.80g, nicotinic acid：0.5~1.2mg, adenine：5.5~10.5mg, FeSO4：3.5~7.5mg；10~15g of agar.The thicker strain of pod membrane is obtained using culture medium of the present invention, beneficial to the yield for improving capsular polysaccharide, production efficiency is improved, reduces production cost.
Description
Technical field
The present invention relates to microbial technology field, and in particular to the solid medium of streptococcus pneumonia.
Background technology
Streptococcus pneumonia (streptococcus pneumoniae) was isolated by Pasteur first in 1881, was great Ye
Property pneumonia, meningitis, one of the principal causative source of bronchitis, it can also cause tympanitis, nasosinusitis and bacteremia etc..Pneumonia
Streptococcal infection is one of major reason for causing death in the world.The U.S. has data to show, and estimation is annual 40~
500000 people suffer from an inflammation of the lungs coccus pneumonia, and case fatality rate is 5%~10%.In it can prevent the disease of death of child with vaccine, pneumonia
Disease ranks the first caused by streptococcus.With a large amount of uses of antibiotic, antibody-resistant bacterium is growing day by day, and medical field is paid close attention to again
The research and development of vaccine.Pneumococcus has now been found that 90 various serotypes.Its bacterium surface pod membrane has antigenicity, then by pneumonia ball
Granulose is covalently attached to carrier protein and makes thymus-dependent (TD) antigen, infant and old man can be produced anti-
Body, immune effect effectively improve.Existing vaccine is to extract its capsular polysaccharide to be made for raw material.
Since pneumovax is researched and developed, because its capsular polysaccharide yield is relatively low, only by expanding the scale of production as far as possible, with
Reach the purpose for improving polysaccharide yield, therefore production cost is higher.Improving polysaccharide yield can be from screening high-quality strain, optimization fermentation
The critical processes such as technique, optimized purification technique are set about.The thicker strain of pod membrane is screened, is one of critical process, from source
Solves this problem.
The content of the invention
The technical problems to be solved by the invention are the capsular polysaccharide yield that existing medium culture streptococcus pneumonia obtains
It is relatively low, and it is an object of the present invention to provide the solid medium of streptococcus pneumonia, the thicker strain of pod membrane, profit are obtained using culture medium of the present invention
In the yield for improving capsular polysaccharide, improve production efficiency, reduce production cost.
The present invention is achieved through the following technical solutions：
The solid medium of streptococcus pneumonia, the formula composition of the solid medium are that solvent is the relative usage of water
For 1000ml：
It is trypticase containing nitrogen source：20~85g, tryptone：25~80g, selected soya peptone：15~70g, soya peptone：20~
85g, acid hydrolyzed casein：15~75g, yeast extract：15~35g, yeast extract：Wherein three kinds of 10~45g；
Carbon source is glucose：15~55g；
Salt is K2HPO4：3.0~15.0g, NaCl：2.0~10.0g, MgSO4·7H2O：0.1~4.0g, CaCl2：
0.10~0.18g；
Trace element is L-cysteine hydrochloride：0.12~1.80g, nicotinic acid：0.5~1.2mg, adenine：5.5~
10.5mg FeSO4：3.5~7.5mg；
10~15g of agar.
Preferably, the formula composition of the solid medium is that solvent is that the relative usage of water is 1000ml：
It is trypticase containing nitrogen source：45~70g, tryptone：35~65g, selected soya peptone：30~55g, soya peptone：40~
60g, acid hydrolyzed casein：35~55g, yeast extract：20~30g, yeast extract：Wherein three kinds of 20~35g；
Carbon source is glucose：25~40g；
Salt is K2HPO4：6.0~9.0g, NaCl：4.0~8.0g, MgSO4·7H2O：0.9~2.1g, CaCl2：0.13
~0.16g；
Trace element is L-cysteine hydrochloride：0.18~1.40g, nicotinic acid：0.6~0.9mg, adenine：6.5~
8.0mg, FeSO4：4.5~6.5mg；
12~15g of agar.
Preferably, the formula composition of the solid medium is that solvent is that the relative usage of water is 1000ml：
It is trypticase containing nitrogen source：60g, tryptone：55g, selected soya peptone：45g, soya peptone：50g, acid hydrolyzed casein：
45g, yeast extract：25g, yeast extract：Wherein three kinds of 25g；
Carbon source is glucose：32g；
Salt is K2HPO4：8.0g, NaCl：6.0g, MgSO4·7H2O：1.5g, CaCl2：0.14g；
Trace element is L-cysteine hydrochloride：1.10g nicotinic acid：0.8mg, adenine：7.0mg, FeSO4：7.5mg；
Agar 13g.
Preferably, it is described to be containing nitrogen source：Tryptone：25~80g, soya peptone：20~85g, acid hydrolyzed casein：15~75g.
Preferably, it is containing nitrogen source：Trypticase：20~85g, soya peptone：20~85g, yeast extract：10~45g.
The present invention compared with prior art, has the following advantages and advantages the solid training of streptococcus pneumonia of the present invention
Support base,
The invention provides a kind of solid medium of streptococcus pneumonia, and it is thicker to obtain pod membrane using culture medium of the present invention
Strain, beneficial to the yield for improving capsular polysaccharide, improve production efficiency, reduce production cost.
Embodiment
For the object, technical solutions and advantages of the present invention are more clearly understood, with reference to embodiment, the present invention is made
Further to describe in detail, exemplary embodiment of the invention and its explanation are only used for explaining the present invention, are not intended as to this
The restriction of invention.
Embodiment 1
The invention provides a kind of solid medium of streptococcus pneumonia, the formula composition of the solid medium is, molten
Agent is that the relative usage of water is 1000ml：
It is trypticase containing nitrogen source：20g, soya peptone：20g, acid hydrolyzed casein：15g；
Carbon source is glucose：15g；
Salt is K2HPO4：3.0g, NaCl：2.0g, MgSO4·7H2O：0.1g, CaCl2：0.10g；
Trace element is L-cysteine hydrochloride：0.12g, nicotinic acid：0.5mg, adenine：5.5mg, FeSO4：3.5mg；
Agar 10g.
Embodiment 2
The invention provides a kind of solid medium of streptococcus pneumonia, the formula composition of the solid medium is, molten
Agent is that the relative usage of water is 1000ml：
It is trypticase containing nitrogen source：85g, selected soya peptone：70g, soya peptone：20~85g；
Carbon source is glucose：55g；
Salt is K2HPO4：15.0g NaCl：10.0g MgSO4·7H2O：4.0g, CaCl2：0.18g；
Trace element is L-cysteine hydrochloride：1.80g nicotinic acid：1.2mg, adenine：10.5mg FeSO4：
7.5mg；
Agar 15g.
Embodiment 3
The invention provides a kind of solid medium of streptococcus pneumonia, the formula composition of the solid medium is, molten
Agent is that the relative usage of water is 1000ml：
It is tryptone containing nitrogen source：35g, soya peptone：40g, yeast extract：20g；
Carbon source is glucose：25g；
Salt is K2HPO4：6.0g, NaCl：4.0g, MgSO4·7H2O：0.9g, CaCl2：0.13g；
Trace element is L-cysteine hydrochloride：0.18g, nicotinic acid：0.6mg, adenine：6.5mg, FeSO4：4.5mg；
Agar 12g.
Embodiment 4
The invention provides a kind of solid medium of streptococcus pneumonia, the formula composition of the solid medium is, molten
Agent is that the relative usage of water is 1000ml：
It is tryptone containing nitrogen source：65g, soya peptone：60g, yeast extract：30g；
Carbon source is glucose：40g；
Salt is K2HPO4：9.0g, NaCl：8.0g, MgSO4·7H2O：2.1g, CaCl2：0.16g；
Trace element is L-cysteine hydrochloride：1.40g nicotinic acid：0.9mg, adenine：8.0mg, FeSO4：6.5mg；
Agar 15g.
Embodiment 5
The invention provides a kind of solid medium of streptococcus pneumonia, the formula composition of the solid medium is, molten
Agent is that the relative usage of water is 1000ml：
It is trypticase containing nitrogen source：60g, selected soya peptone：45g, yeast extract：25g；
Carbon source is glucose：32g；
Salt is K2HPO4：8.0g, NaCl：6.0g, MgSO4·7H2O：1.5g, CaCl2：0.14g；
Trace element is L-cysteine hydrochloride：1.10g nicotinic acid：0.8mg, adenine：7.0mg, FeSO4：7.5mg；
Agar 13g.
Above-described embodiment, the purpose of the present invention, technical scheme and beneficial effect are carried out further
Describe in detail, should be understood that the embodiment that the foregoing is only the present invention, be not intended to limit the present invention
Protection domain, within the spirit and principles of the invention, any modification, equivalent substitution and improvements done etc., all should include
Within protection scope of the present invention.
Claims (5)
1. the solid medium of streptococcus pneumonia, it is characterised in that the formula composition of the solid medium is that solvent is water
Relative usage is 1000ml：
It is trypticase containing nitrogen source：20~85g, tryptone：25~80g, selected soya peptone：15~70g, soya peptone：20~85g, acid
Caseinhydrolysate：15~75g, yeast extract：15~35g, yeast extract：Wherein three kinds of 10~45g；
Carbon source is glucose：15~55g；
Salt is K2HPO4：3.0~15.0g, NaCl：2.0~10.0g, MgSO4·7H2O：0.1~4.0g, CaCl2：0.10~
0.18g；
Trace element is L-cysteine hydrochloride：0.12~1.80g, nicotinic acid：0.5~1.2mg, adenine：5.5~
10.5mg FeSO4：3.5~7.5mg；
10~15g of agar.
2. the solid medium of streptococcus pneumonia according to claim 1, it is characterised in that the solid medium is matched somebody with somebody
Fang Zucheng is that solvent is that the relative usage of water is 1000ml：
It is trypticase containing nitrogen source：45~70g, tryptone：35~65g, selected soya peptone：30~55g, soya peptone：40~60g, acid
Caseinhydrolysate：35~55g, yeast extract：20~30g, yeast extract：Wherein three kinds of 20~35g；
Carbon source is glucose：25~40g；
Salt is K2HPO4：6.0~9.0g, NaCl：4.0~8.0g, MgSO4·7H2O：0.9~2.1g, CaCl2：0.13~
0.16g；
Trace element is L-cysteine hydrochloride：0.18~1.40g, nicotinic acid：0.6~0.9mg, adenine：6.5~8.0mg,
FeSO4：4.5~6.5mg；
12~15g of agar.
3. the solid medium of streptococcus pneumonia according to claim 1, it is characterised in that the solid medium is matched somebody with somebody
Fang Zucheng is that solvent is that the relative usage of water is 1000ml：
It is trypticase containing nitrogen source：60g, tryptone：55g, selected soya peptone：45g, soya peptone：50g, acid hydrolyzed casein：45g, ferment
Female extract：25g, yeast extract：Wherein three kinds of 25g；
Carbon source is glucose：32g；
Salt is K2HPO4：8.0g, NaCl：6.0g, MgSO4·7H2O：1.5g, CaCl2：0.14g；
Trace element is L-cysteine hydrochloride：1.10g nicotinic acid：0.8mg, adenine：7.0mg, FeSO4：7.5mg；
Agar 13g.
4. the solid medium of streptococcus pneumonia according to claim 1, it is characterised in that described to be containing nitrogen source：Tryptone：
25~80g, soya peptone：20~85g, acid hydrolyzed casein：15~75g.
5. the solid medium of streptococcus pneumonia according to claim 1, it is characterised in that be containing nitrogen source：Trypticase：20
~85g, soya peptone：20~85g, yeast extract：10~45g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711235436.9A CN107760633A (en) | 2017-11-30 | 2017-11-30 | The solid medium of streptococcus pneumonia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711235436.9A CN107760633A (en) | 2017-11-30 | 2017-11-30 | The solid medium of streptococcus pneumonia |
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| Publication Number | Publication Date |
|---|---|
| CN107760633A true CN107760633A (en) | 2018-03-06 |
Family
ID=61276321
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711235436.9A Pending CN107760633A (en) | 2017-11-30 | 2017-11-30 | The solid medium of streptococcus pneumonia |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5314822A (en) * | 1992-10-15 | 1994-05-24 | Merck & Co., Inc. | Method of clonal growth of Streptococcus pneumoniae |
| CN101932698A (en) * | 2007-12-20 | 2010-12-29 | 诺华有限公司 | Fermentation processes for cultivating streptococci and purification processes for obtaining cps therefrom |
| WO2016207774A1 (en) * | 2015-06-23 | 2016-12-29 | Politecnico Di Torino | Method and device for searching images |
| CN106479930A (en) * | 2016-11-04 | 2017-03-08 | 哈尔滨亿隆科技有限公司 | A kind of improved formulations of TSA culture medium |
-
2017
- 2017-11-30 CN CN201711235436.9A patent/CN107760633A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5314822A (en) * | 1992-10-15 | 1994-05-24 | Merck & Co., Inc. | Method of clonal growth of Streptococcus pneumoniae |
| CN101932698A (en) * | 2007-12-20 | 2010-12-29 | 诺华有限公司 | Fermentation processes for cultivating streptococci and purification processes for obtaining cps therefrom |
| WO2016207774A1 (en) * | 2015-06-23 | 2016-12-29 | Politecnico Di Torino | Method and device for searching images |
| CN106479930A (en) * | 2016-11-04 | 2017-03-08 | 哈尔滨亿隆科技有限公司 | A kind of improved formulations of TSA culture medium |
Non-Patent Citations (2)
| Title |
|---|
| V.M. GONÇALVES等: "Optimization of medium and cultivation conditions", 《APPLIED MICROBIOLOGY BIOTECHNOLOGY》 * |
| 王剑虹等: "肺炎链球菌培养研究", 《微生物学免疫学进展》 * |
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Application publication date: 20180306 |
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