CN107759648A - A kind of method that Hyperoside and isoquercitrin are isolated and purified from Golden flower - Google Patents
A kind of method that Hyperoside and isoquercitrin are isolated and purified from Golden flower Download PDFInfo
- Publication number
- CN107759648A CN107759648A CN201711061100.5A CN201711061100A CN107759648A CN 107759648 A CN107759648 A CN 107759648A CN 201711061100 A CN201711061100 A CN 201711061100A CN 107759648 A CN107759648 A CN 107759648A
- Authority
- CN
- China
- Prior art keywords
- hyperoside
- isoquercitrin
- golden flower
- purified
- isolated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
- C07H17/06—Benzopyran radicals
- C07H17/065—Benzo[b]pyrans
- C07H17/07—Benzo[b]pyran-4-ones
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/07—Optical isomers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention relates to a kind of method that Hyperoside and isoquercitrin are isolated and purified from Golden flower, belongs to native compound extraction and separation field.Method is as follows：Flavones in heating and refluxing extraction Golden flower；Under alkalescence condition, zinc salt forms stable comple precipitation with the Flavonoid substances in Golden flower in addition to Hyperoside and isoquercitrin；Centrifugation removes complex compound sediment；Concentrated supernatant, obtain Hyperoside and the isoquercitrin of high-purity.This method fast, efficient, safe can isolate and purify Hyperoside and isoquercitrin from Golden flower, help to realize the industrial production of Hyperoside and isoquercitrin.
Description
Technical field
The present invention relates to a kind of method that Hyperoside and isoquercitrin are isolated and purified from Golden flower.Specifically, from
Golden flower extract solution sets out, weaker using Hyperoside and isoquercitrin and zinc salt complexing power, other flavone compounds with
The stronger characteristic of zinc salt complexing power, other flavone compounds in Golden flower extract solution are removed, developed a kind of quick, high
The method that effect, safety isolate and purify Hyperoside and isoquercitrin from Golden flower.Belong to native compound extraction and separation neck
Domain.
Background technology
Hyperoside also known as Quercetin -3-O- β-D- galactopyranosides, isoquercitrin also known as Quercetin -3-O- β-D- pyrroles
The glucosides of glucopyranoside glycosides, both Quercetin, there is identical aglycon and similar polarity, only have one in glycosyl part
The configuration of hydroxyl is different, and the former is galactoside, and the latter is glucoside, and remainder is identical, belongs to flavonols glycoside
Compound.(Fudan Journal, 2007,46,417-420) they are all widely present in various plants, such as the capsule of weeping forsythia, Sunset Abelmoschus Root
Flower, evodia rutaecarpa and Folium Apocyni Veneti etc., are all important natural products.Many pharmaceutical researches show that Hyperoside has improvement
Effect (the middle traditional Chinese medicines such as cardiovascular function, cerebral ischemia/reperfusion injury protection, anti-oxidant reduction, anti-inflammatory, analgesia cough-relieving, decompression
Room, 2016,27,1415-1417), isoquercitrin has the pharmacological activities such as hypotensive, anti-inflammatory, antidepression, and (pharmacy practice is miscellaneous
Will, 2011,29,272-274).The structural formula of Hyperoside is as shown in following formula I, and the structural formula of isoquercitrin is as shown in following formula II.
Golden flower (Hibiscus manihot L.) alias vegetable Furong, wild lotus, glutinous dry or wych-elm skin, are annual grass
This Malvaceae (Malvaceae) gumbo platymiscium, Golden flower have the good reputation of " plant giant panda ", contain various plants bioactivity
Material, mainly having flavone compound, vitamin E, unrighted acid, dietary fiber and trace element etc., (Chinese medicine is commented
Valency, 2015,32,90-92).In recent years, flower of JINHUAKUI is paid close attention to as the important sources of extraction Flavonoid substances by each side.It is existing
Research shows, Hyperoside and isoquercitrin are the main actives in flower of JINHUAKUI, and its highest content is respectively 1.59%
With 1.31% (Beijing University of Chinese Medicine's journal, 2016,39,308-315).
There are much reports on isolating and purifying Hyperoside and isoquercitrin at present.For example the summer passes the (Chinese patents such as sea
Application number 201210424557.9) made simultaneously from Folium Apocyni Veneti using the mode of the macroporous resin adsorption elution of thtee-stage shiplock
Standby Hyperoside and isoquercitrin, purity reach 90%-98%.(the Chinese Patent Application No. such as Fu Wangjie
201110091575.1) it is pure using macroporous resin adsorption, the mode of silica gel column chromatography purifies and separates Hyperoside from wintergreen
Degree can reach 95%.These methods all have that purification process is cumbersome, cost is too high, the production cycle is long, is difficult to industrial production etc. asks
Topic, limits the application of Hyperoside and isoquercitrin.
The present invention is weaker using Hyperoside and isoquercitrin and zinc salt complexing power from Golden flower extract solution, its
His flavone compound and the stronger characteristic of zinc salt complexing power, remove other flavone compounds in Golden flower extract solution,
Develop a kind of fast, efficient, safe method that Hyperoside and isoquercitrin are isolated and purified from Golden flower.
The content of the invention
The purpose of the present invention is, other flavonoids weaker using Hyperoside and isoquercitrin and zinc salt complexing power
Thing and the stronger characteristic of zinc salt complexing power, remove other flavone compounds in Golden flower extract solution, develop it is a kind of it is quick,
Efficiently, the safe method for isolating and purifying Hyperoside-isoquercitrin composition in Golden flower.
The purpose of the present invention is realized by following technical scheme：
It is a kind of from Golden flower extract solution, it is different using Flavonoid substances in Golden flower and zinc salt complexing power power,
A kind of method for developing fast, efficient, safe Hyperoside isolated and purified in Golden flower and isoquercitrin.Its step is such as
Under：
A. precision weighs 5.0g and dries flower of JINHUAKUI corase meal, under conditions of 80 DEG C of temperature, with 70% ethanol solution
Heating and refluxing extraction 3h, filtered after being cooled to room temperature, obtain Golden flower residue and supernatant, supernatant is collected, with 70% second
Alcoholic solution constant volume produces sample solution into 250mL volumetric flasks；
B. Golden flower sample solution is weighed, it is 8.5-12.5 to adjust pH value with alkaline solution, preferably 10.5, alkalescence used
Finite concentration potassium hydroxide solution, sodium hydroxide solution, sodium carbonate liquor, ammoniacal liquor etc., preferably potassium hydroxide may be selected in solution
Solution.Certain mass concentration zinc salt is added, zinc sulfate, zinc acetate, zinc chloride etc., preferably zinc sulfate may be selected in zinc salt used,
Addition ensures that the mass ratio of Golden flower sample and zinc salt is 1/0.5-1/5, preferably 1/1.After addition, the complex reaction time
For 1-12h, preferably 3-5h.Complex reaction temperature is 10-70 DEG C, preferably 30-40 DEG C, centrifuges, removes zinc salt-Huang
Ketone complex compound sediment, collect supernatant；
C. above-mentioned supernatant is heated into concentration, obtains Hyperoside and the isoquercitrin of high-purity.
Hyperoside and isoquercitrin to the extract solution of Golden flower and after isolating and purifying obtain through efficient liquid phase chromatographic analysis
To analysis result, chromatogram is shown in accompanying drawing.The chromatographic condition used for：Waters HPLC high performance liquid chromatographs, chromatographic column are
COSMOSIL C18 (4.6mm × 200mm, 5 μm), mobile phase be the formic acid solution of acetonitrile -0.1% gradient elution (0min, 15:
85v/v；18min, 16:84v/v；26min, 20:80v/v；35min, 40:60v/v；40min, 40:60v/v), flow velocity is
1.0m L/min, Detection wavelength 360nm, the μ L of sample size 10, column temperature is room temperature.
The present invention achieves following useful achievement：
1st, Golden flower extract solution need not be further processed, is directly added into zinc salt, make zinc salt with carrying by adjusting pH
Take other Flavonoid substances in liquid in addition to Hyperoside and isoquercitrin to be complexed, the spun gold in Golden flower can be isolated and purified simultaneously
Peach glycosides and isoquercitrin, processing step is simplified, save operating time, the consumption for reducing manpower and the energy etc., improve preparation
Efficiency and economic benefit；
2nd, the cost using zinc salts such as zinc sulfate is low, nontoxic, and degree of danger reduces, and also allows for returning for Extraction solvent
Receive；
3rd, operated by aforementioned combinatorial, the extensive work that the Hyperoside and isoquercitrin being easy in Golden flower isolate and purify
Industry produces.
Brief description of the drawings
Fig. 1 is the HPLC spectrograms of Hyperoside and isoquercitrin in not purified Golden flower extract solution.
Fig. 2 is HPLC spectrograms of Hyperoside and isoquercitin in Golden flower extract solution after purification.
Embodiment：
One kind of the present invention is isolated and purified from Golden flower with specific embodiment below in conjunction with the accompanying drawings Hyperoside and
The further explanation of the method for isoquercitrin, so that those skilled in the art becomes more apparent upon the present invention, but do not limited with this
The system present invention.
Embodiment 1：
A. precision weighs 5.0g and dries flower of JINHUAKUI corase meal, under conditions of 80 DEG C of temperature, with 70% ethanol solution
Heating and refluxing extraction 3h, filtered after being cooled to room temperature, obtain Golden flower residue and supernatant, supernatant is collected, with 70% second
Alcoholic solution constant volume produces sample solution into 250mL volumetric flasks；
B. 10mL Golden flower sample solutions are measured, is 10.5 with potassium hydroxide solution regulation pH value, it is dense to add certain mass
Zinc sulfate is spent, addition ensures that the mass ratio of Golden flower sample and zinc sulfate is 1/1.After addition, the complex reaction time is 3h.Network
It is 30 DEG C to close reaction temperature, is centrifuged, and obtains supernatant and zinc salt-flavones complex compound, collects supernatant；
C. above-mentioned supernatant is heated into concentration, obtains Hyperoside and the isoquercitrin of high-purity, Hyperoside and different
Quercetin purity is respectively 46.59% and 33.55%.
Embodiment 2：
A. precision weighs 5.0g and dries flower of JINHUAKUI corase meal, under conditions of 80 DEG C of temperature, with 70% ethanol solution
Heating and refluxing extraction 3h, filtered after being cooled to room temperature, obtain Golden flower residue and supernatant, supernatant is collected, with 70% second
Alcoholic solution constant volume produces sample solution into 250mL volumetric flasks；
B. 10mL Golden flower sample solutions are measured, is 12.5 with sodium carbonate liquor regulation pH value, adds certain mass concentration
Zinc chloride, addition ensure that the mass ratio of Golden flower sample and zinc chloride is 1/0.5.After addition, the complex reaction time is 12h.
Complex reaction temperature is 40 DEG C, is centrifuged, and obtains supernatant and zinc salt-flavones complex compound, collects supernatant；
C. above-mentioned supernatant is heated into concentration, obtains Hyperoside and the isoquercitrin of high-purity, Hyperoside and different
Quercetin purity is respectively 43.14% and 28.44%.
Embodiment 3：
A. precision weighs 5.0g and dries flower of JINHUAKUI corase meal, under conditions of 80 DEG C of temperature, with 70% ethanol solution
Heating and refluxing extraction 3h, filtered after being cooled to room temperature, obtain Golden flower residue and supernatant, supernatant is collected, with 70% second
Alcoholic solution constant volume produces sample solution into 250mL volumetric flasks；
B. 10mL Golden flower sample solutions are measured, is 11.5 with sodium hydroxide solution regulation pH value, it is dense to add certain mass
Zinc acetate is spent, addition ensures that the mass ratio of Golden flower sample and zinc acetate is 1/5.After addition, the complex reaction time is 1h.Network
It is 10 DEG C to close reaction temperature, is centrifuged, and obtains supernatant and zinc salt-flavones complex compound, collects supernatant；
C. above-mentioned supernatant is heated into concentration, obtains Hyperoside and the isoquercitrin of high-purity, Hyperoside and different
Quercetin purity is respectively 42.97% and 28.24%.
Embodiment 4：
A. precision weighs 5.0g and dries flower of JINHUAKUI corase meal, under conditions of 80 DEG C of temperature, with 70% ethanol solution
Heating and refluxing extraction 3h, filtered after being cooled to room temperature, obtain Golden flower residue and supernatant, supernatant is collected, with 70% second
Alcoholic solution constant volume produces sample solution into 250mL volumetric flasks；
B. 10mL Golden flower sample solutions are measured, is 10.5 with ammoniacal liquor regulation pH value, adds certain mass concentration sulphuric acid zinc,
Addition ensures that the mass ratio of Golden flower sample and zinc sulfate is 1/0.5.After addition, the complex reaction time is 5h.Complex reaction
Temperature is 70 DEG C, and after addition, the complex reaction time is 1h.Complex reaction temperature is 10 DEG C, centrifuges, obtains supernatant and zinc
Salt-flavones complex compound, collect supernatant；
C. above-mentioned supernatant is heated into concentration, obtains Hyperoside and the isoquercitrin of high-purity, Hyperoside and different
Quercetin purity is respectively 44.43% and 30.74%.
Embodiment 5：
A. precision weighs 5.0g and dries flower of JINHUAKUI corase meal, under conditions of 80 DEG C of temperature, with 70% ethanol solution
Heating and refluxing extraction 3h, filtered after being cooled to room temperature, obtain Golden flower residue and supernatant, supernatant is collected, with 70% second
Alcoholic solution constant volume produces sample solution into 250mL volumetric flasks；
B. 10mL Golden flower sample solutions are measured, is 10.5 with potassium hydroxide regulation pH value, adds certain mass concentration sulphur
Sour zinc, addition ensure that the mass ratio of Golden flower sample and zinc sulfate is 1/1.After addition, the complex reaction time is 5h.Complexing is anti-
It is 40 DEG C to answer temperature, is centrifuged, and obtains supernatant and zinc salt-flavones complex compound, collects supernatant；
C. above-mentioned supernatant is heated into concentration, obtains Hyperoside and the isoquercitrin of high-purity, Hyperoside and different
Quercetin purity is respectively 44.07% and 28.39%.
Embodiment 6：
A. precision weighs 5.0g and dries flower of JINHUAKUI corase meal, under conditions of 80 DEG C of temperature, with 70% ethanol solution
Heating and refluxing extraction 3h, filtered after being cooled to room temperature, obtain Golden flower residue and supernatant, supernatant is collected, with 70% second
Alcoholic solution constant volume produces sample solution into 250mL volumetric flasks；
B. 10mL Golden flower sample solutions are measured, is 8.5 with hydrogen-oxygen potassium regulation pH value, adds certain mass concentration sulphuric acid
Zinc, addition ensure that the mass ratio of Golden flower sample and zinc sulfate is 1/2.After addition, the complex reaction time is 7h.Complex reaction
Temperature is 50 DEG C, is centrifuged, and obtains supernatant and zinc salt-flavones complex compound, collects supernatant；
C. above-mentioned supernatant is heated into concentration, obtains Hyperoside and the isoquercitrin of high-purity, Hyperoside and different
Quercetin purity is respectively 39.10% and 21.70%.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc., it should be included in the scope of the protection.
Claims (7)
- A kind of 1. method that Hyperoside and isoquercitrin are isolated and purified from Golden flower, it is characterised in that：A. precision weighs 5.0g and dries flower of JINHUAKUI corase meal, under conditions of 80 DEG C of temperature, is heated with 70% ethanol solution Refluxing extraction 3h, filtered after being cooled to room temperature, obtain Golden flower residue and supernatant, collect supernatant, it is molten with 70% ethanol Liquid constant volume produces sample solution into 250mL volumetric flasks；B. Golden flower sample solution is weighed, it is 8.5-12.5 to adjust pH value with alkaline solution, and alkaline solution used may be selected certain Concentration potassium hydroxide solution, sodium hydroxide solution, sodium carbonate liquor, ammoniacal liquor etc..Add certain mass concentration zinc salt, zinc salt used Zinc sulfate, zinc acetate, zinc chloride etc. may be selected, addition ensures that the mass ratio of Golden flower sample and zinc salt is 1/0.5-1/5.Add After adding, the complex reaction time is 1-12h.Complex reaction temperature is 10-70 DEG C, centrifuges removal zinc salt-flavones complex compound and sinks Form sediment, collect supernatant；C. above-mentioned supernatant is heated into concentration, obtains Hyperoside and the isoquercitrin of high-purity.
- 2. a kind of method that Hyperoside and isoquercitrin are isolated and purified from Golden flower according to claim 1, it is special Sign is：Finite concentration potassium hydroxide solution, sodium hydroxide solution, sodium carbonate liquor, ammoniacal liquor etc. may be selected in alkaline solution, preferably For potassium hydroxide solution.
- 3. a kind of method that Hyperoside and isoquercitrin are isolated and purified from Golden flower according to claim 1, it is special Sign is：Regulation pH value is 8.5-12.5, preferably 10.5.
- 4. a kind of method that Hyperoside and isoquercitrin are isolated and purified from Golden flower according to claim 1, it is special Sign is：Zinc sulfate, zinc acetate, zinc chloride etc., preferably zinc sulfate may be selected in zinc salt used in complex reaction.
- 5. a kind of method that Hyperoside and isoquercitrin are isolated and purified from Golden flower according to claim 1, it is special Sign is：The mass ratio of Golden flower sample and zinc salt is 1/0.5-1/5 in complex reaction, preferably 1/1.
- 6. a kind of method that Hyperoside and isoquercitrin are isolated and purified from Golden flower according to claim 1, it is special Sign is：The complex reaction time is 1-12h, preferably 3-5h.
- 7. a kind of method that Hyperoside and isoquercitrin are isolated and purified from Golden flower according to claim 1, it is special Sign is：Complex reaction temperature is 10-70 DEG C, preferably 30-40 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711061100.5A CN107759648B (en) | 2017-11-02 | 2017-11-02 | Method for separating and purifying hyperoside and isoquercitrin from abelmoschus manihot |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711061100.5A CN107759648B (en) | 2017-11-02 | 2017-11-02 | Method for separating and purifying hyperoside and isoquercitrin from abelmoschus manihot |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107759648A true CN107759648A (en) | 2018-03-06 |
| CN107759648B CN107759648B (en) | 2020-09-11 |
Family
ID=61271990
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711061100.5A Active CN107759648B (en) | 2017-11-02 | 2017-11-02 | Method for separating and purifying hyperoside and isoquercitrin from abelmoschus manihot |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107759648B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108864226A (en) * | 2018-08-09 | 2018-11-23 | 湖南农业大学 | A method of extracting ardisin and quercitrin from ardisia japonica |
| CN109810116A (en) * | 2019-02-26 | 2019-05-28 | 北京理工大学 | A method of ellagic acid in purifying Chinese chestnut hair shell extracting solution |
| CN110590882A (en) * | 2019-09-16 | 2019-12-20 | 北京理工大学 | Method for simultaneously separating and purifying 6 flavone compounds from sunflower seeds |
| CN115669470A (en) * | 2020-12-11 | 2023-02-03 | 北京林业大学 | Application of AemYB30 and/or AeUF3GaT1 in improving seting rate of Abelmoschus manihot |
| CN117442534A (en) * | 2023-12-14 | 2024-01-26 | 广州嘉瑜生物科技有限公司 | Abelmoschus manihot extract, preparation method thereof and application thereof in preparing skin care product |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103018371A (en) * | 2012-12-24 | 2013-04-03 | 江苏苏中药业集团股份有限公司 | Quality control method of sunset abelmoschus root medicinal material as well as extract and preparation of sunset abelmoschus root |
-
2017
- 2017-11-02 CN CN201711061100.5A patent/CN107759648B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103018371A (en) * | 2012-12-24 | 2013-04-03 | 江苏苏中药业集团股份有限公司 | Quality control method of sunset abelmoschus root medicinal material as well as extract and preparation of sunset abelmoschus root |
Non-Patent Citations (6)
| Title |
|---|
| G. LE NEST，等: "Zn–polyphenol chelation: complexes with quercetin,(+)-catechin, and derivatives: I optical and NMR studies", 《INORGANICA CHIMICA ACTA》 * |
| JING ZHANG，等: "Purification of flavonoid fromGingko bilobaextract by zinc complexation method and its effect on antioxidant activity", 《SEPARATION AND PURIFICATION TECHNOLOGY》 * |
| 冯朵，等: "基于药物体系的金花葵特征图谱质量表征关联分析研究", 《北京中医药大学学报》 * |
| 李春梅，等: "中药黄蜀葵花化学成分的分离与鉴定(Ⅱ)", 《沈阳药科大学学报》 * |
| 林华婷，等: "金属络合法纯化鼠曲草类黄酮工艺", 《中国食品学报》 * |
| 温锐，等: "黄蜀葵化学成分与药理活性研究进展", 《中国野生植物资源》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108864226A (en) * | 2018-08-09 | 2018-11-23 | 湖南农业大学 | A method of extracting ardisin and quercitrin from ardisia japonica |
| CN108864226B (en) * | 2018-08-09 | 2021-05-07 | 湖南农业大学 | Method for extracting ardisiacrispin and quercitrin from Japanese ardisia |
| CN109810116A (en) * | 2019-02-26 | 2019-05-28 | 北京理工大学 | A method of ellagic acid in purifying Chinese chestnut hair shell extracting solution |
| CN109810116B (en) * | 2019-02-26 | 2021-06-04 | 北京理工大学 | Method for purifying ellagic acid in chestnut shell extracting solution |
| CN110590882A (en) * | 2019-09-16 | 2019-12-20 | 北京理工大学 | Method for simultaneously separating and purifying 6 flavone compounds from sunflower seeds |
| CN110590882B (en) * | 2019-09-16 | 2020-11-03 | 北京理工大学 | Method for simultaneously separating and purifying 6 flavone compounds from sunflower seeds |
| CN115669470A (en) * | 2020-12-11 | 2023-02-03 | 北京林业大学 | Application of AemYB30 and/or AeUF3GaT1 in improving seting rate of Abelmoschus manihot |
| CN117442534A (en) * | 2023-12-14 | 2024-01-26 | 广州嘉瑜生物科技有限公司 | Abelmoschus manihot extract, preparation method thereof and application thereof in preparing skin care product |
| CN117442534B (en) * | 2023-12-14 | 2024-05-28 | 广州嘉瑜生物科技有限公司 | Abelmoschus manihot extract, preparation method thereof and application thereof in preparing skin care product |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107759648B (en) | 2020-09-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107759648A (en) | A kind of method that Hyperoside and isoquercitrin are isolated and purified from Golden flower | |
| CN105001281B (en) | A kind of industrialized preparing process of synchronous production steviol glycoside, flavones and chlorogenic acid | |
| CN1324043C (en) | Prepn and use of high-purity momordica glycoside V | |
| CN101220062A (en) | Method for preparing stevioside and rebaudiodside A simultaneously | |
| CN101353363B (en) | Method for separating and purifying Momordica grosvenori leaf chromocor compound by high-speed countercurrent chromatography and products thereof | |
| CN104906153A (en) | Technological method for efficiently extracting ginkgo flavone | |
| CN103110670A (en) | Preparation method for efficiently extracting separating high-purity flavone components from ginkgo leaf | |
| CN106198172B (en) | A kind of selective enrichment method of tree peony anthocyanins in black fruit fructus lycii | |
| CN110623988A (en) | Method for extracting and preparing total flavonoids of cotton rose leaves | |
| CN102643315B (en) | Method for purifying phlorizin from apple velamen | |
| CN104557834B (en) | A kind of isolated and purified pinocembrin, Chrysin and method of Galangin from China's Water extracts of propolis | |
| CN102942606B (en) | Method for preparing high-purity lycium barbarum acid | |
| CN101891727A (en) | Method for separating and extracting apigenin, acacetin-7-O-beta-D-glucoside and apigenin-7-O-beta-D-glucoside from dendranthema indicum | |
| CN102627679A (en) | Method for preparing schaftoside from desmodium styracifolium | |
| CN104761596A (en) | Method for preparing anthocyanin standard substance | |
| CN104311616B (en) | A kind of extraction high purity cortex fraxini and method of fraxin from Cortex Fraxini | |
| CN106539848A (en) | A kind of preparation method of sweet persimmon flavone | |
| CN113354697B (en) | Method for separating and purifying pectolinarin and linarin | |
| CN107188772B (en) | Urea derivative aqueous two-phase system and application thereof | |
| CN102942455B (en) | Method for extracting oxyresveratrol from mulberry branches | |
| CN104230871A (en) | Method for separating polymethoxylated flavones, hesperidin and synephrine from immature bitter orange | |
| CN101618052A (en) | Process for extracting total flavonoids from hippophae leaves | |
| CN102285954B (en) | Method for extracting epicatechin from red bayberry fruit residues | |
| CN104650164A (en) | Method for preparing active flavonoid glycoside monomers from pepper leaf | |
| CN103242399A (en) | Method for extracting apigenin-7-O-beta-D-glucuronide from broussonetia papyrifera leaves |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |