CN107759577A - GS5816 intermediates, preparation method and application - Google Patents
GS5816 intermediates, preparation method and application Download PDFInfo
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- CN107759577A CN107759577A CN201710919030.6A CN201710919030A CN107759577A CN 107759577 A CN107759577 A CN 107759577A CN 201710919030 A CN201710919030 A CN 201710919030A CN 107759577 A CN107759577 A CN 107759577A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 206
- FHCUMDQMBHQXKK-CDIODLITSA-N velpatasvir Chemical compound C1([C@@H](NC(=O)OC)C(=O)N2[C@@H](C[C@@H](C2)COC)C=2NC(=CN=2)C=2C=C3C(C4=CC5=CC=C6NC(=NC6=C5C=C4OC3)[C@H]3N([C@@H](C)CC3)C(=O)[C@@H](NC(=O)OC)C(C)C)=CC=2)=CC=CC=C1 FHCUMDQMBHQXKK-CDIODLITSA-N 0.000 title abstract description 14
- 229960000863 velpatasvir Drugs 0.000 title abstract description 14
- 239000000543 intermediate Substances 0.000 title abstract description 10
- 150000001875 compounds Chemical class 0.000 claims abstract description 88
- 238000006243 chemical reaction Methods 0.000 claims abstract description 77
- GHYOCDFICYLMRF-UTIIJYGPSA-N (2S,3R)-N-[(2S)-3-(cyclopenten-1-yl)-1-[(2R)-2-methyloxiran-2-yl]-1-oxopropan-2-yl]-3-hydroxy-3-(4-methoxyphenyl)-2-[[(2S)-2-[(2-morpholin-4-ylacetyl)amino]propanoyl]amino]propanamide Chemical compound C1(=CCCC1)C[C@@H](C(=O)[C@@]1(OC1)C)NC([C@H]([C@@H](C1=CC=C(C=C1)OC)O)NC([C@H](C)NC(CN1CCOCC1)=O)=O)=O GHYOCDFICYLMRF-UTIIJYGPSA-N 0.000 claims abstract description 54
- 229940125797 compound 12 Drugs 0.000 claims abstract description 54
- 238000006482 condensation reaction Methods 0.000 claims abstract description 41
- 239000003960 organic solvent Substances 0.000 claims abstract description 41
- UNILWMWFPHPYOR-KXEYIPSPSA-M 1-[6-[2-[3-[3-[3-[2-[2-[3-[[2-[2-[[(2r)-1-[[2-[[(2r)-1-[3-[2-[2-[3-[[2-(2-amino-2-oxoethoxy)acetyl]amino]propoxy]ethoxy]ethoxy]propylamino]-3-hydroxy-1-oxopropan-2-yl]amino]-2-oxoethyl]amino]-3-[(2r)-2,3-di(hexadecanoyloxy)propyl]sulfanyl-1-oxopropan-2-yl Chemical compound O=C1C(SCCC(=O)NCCCOCCOCCOCCCNC(=O)COCC(=O)N[C@@H](CSC[C@@H](COC(=O)CCCCCCCCCCCCCCC)OC(=O)CCCCCCCCCCCCCCC)C(=O)NCC(=O)N[C@H](CO)C(=O)NCCCOCCOCCOCCCNC(=O)COCC(N)=O)CC(=O)N1CCNC(=O)CCCCCN\1C2=CC=C(S([O-])(=O)=O)C=C2CC/1=C/C=C/C=C/C1=[N+](CC)C2=CC=C(S([O-])(=O)=O)C=C2C1 UNILWMWFPHPYOR-KXEYIPSPSA-M 0.000 claims abstract description 39
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 29
- 239000002904 solvent Substances 0.000 claims description 98
- PAQZWJGSJMLPMG-UHFFFAOYSA-N 2,4,6-tripropyl-1,3,5,2$l^{5},4$l^{5},6$l^{5}-trioxatriphosphinane 2,4,6-trioxide Chemical compound CCCP1(=O)OP(=O)(CCC)OP(=O)(CCC)O1 PAQZWJGSJMLPMG-UHFFFAOYSA-N 0.000 claims description 34
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 32
- SZUVGFMDDVSKSI-WIFOCOSTSA-N (1s,2s,3s,5r)-1-(carboxymethyl)-3,5-bis[(4-phenoxyphenyl)methyl-propylcarbamoyl]cyclopentane-1,2-dicarboxylic acid Chemical compound O=C([C@@H]1[C@@H]([C@](CC(O)=O)([C@H](C(=O)N(CCC)CC=2C=CC(OC=3C=CC=CC=3)=CC=2)C1)C(O)=O)C(O)=O)N(CCC)CC(C=C1)=CC=C1OC1=CC=CC=C1 SZUVGFMDDVSKSI-WIFOCOSTSA-N 0.000 claims description 28
- 229940126543 compound 14 Drugs 0.000 claims description 28
- 229910052757 nitrogen Inorganic materials 0.000 claims description 25
- 239000007789 gas Substances 0.000 claims description 24
- 239000002253 acid Substances 0.000 claims description 18
- -1 Phosphonium hexafluorophosphate Chemical compound 0.000 claims description 17
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 claims description 16
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 16
- 239000003513 alkali Substances 0.000 claims description 16
- 230000001681 protective effect Effects 0.000 claims description 14
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-dimethylformamide Substances CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 13
- 150000001408 amides Chemical group 0.000 claims description 13
- 150000002148 esters Chemical class 0.000 claims description 13
- 150000007529 inorganic bases Chemical class 0.000 claims description 12
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 11
- 125000006239 protecting group Chemical group 0.000 claims description 11
- QBWKPGNFQQJGFY-QLFBSQMISA-N 3-[(1r)-1-[(2r,6s)-2,6-dimethylmorpholin-4-yl]ethyl]-n-[6-methyl-3-(1h-pyrazol-4-yl)imidazo[1,2-a]pyrazin-8-yl]-1,2-thiazol-5-amine Chemical compound N1([C@H](C)C2=NSC(NC=3C4=NC=C(N4C=C(C)N=3)C3=CNN=C3)=C2)C[C@H](C)O[C@H](C)C1 QBWKPGNFQQJGFY-QLFBSQMISA-N 0.000 claims description 9
- 229940125846 compound 25 Drugs 0.000 claims description 9
- 229910052786 argon Inorganic materials 0.000 claims description 8
- JMMWKPVZQRWMSS-UHFFFAOYSA-N isopropanol acetate Natural products CC(C)OC(C)=O JMMWKPVZQRWMSS-UHFFFAOYSA-N 0.000 claims description 7
- 229940011051 isopropyl acetate Drugs 0.000 claims description 7
- GWYFCOCPABKNJV-UHFFFAOYSA-N isovaleric acid Chemical group CC(C)CC(O)=O GWYFCOCPABKNJV-UHFFFAOYSA-N 0.000 claims description 7
- 239000002585 base Substances 0.000 claims description 6
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 claims description 6
- 229910000041 hydrogen chloride Inorganic materials 0.000 claims description 6
- 150000007522 mineralic acids Chemical class 0.000 claims description 6
- XRWSZZJLZRKHHD-WVWIJVSJSA-N asunaprevir Chemical compound O=C([C@@H]1C[C@H](CN1C(=O)[C@@H](NC(=O)OC(C)(C)C)C(C)(C)C)OC1=NC=C(C2=CC=C(Cl)C=C21)OC)N[C@]1(C(=O)NS(=O)(=O)C2CC2)C[C@H]1C=C XRWSZZJLZRKHHD-WVWIJVSJSA-N 0.000 claims description 5
- 229940125961 compound 24 Drugs 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 125000001309 chloro group Chemical group Cl* 0.000 claims description 4
- 150000003839 salts Chemical class 0.000 claims description 4
- 125000005931 tert-butyloxycarbonyl group Chemical group [H]C([H])([H])C(OC(*)=O)(C([H])([H])[H])C([H])([H])[H] 0.000 claims description 4
- 125000000217 alkyl group Chemical group 0.000 claims description 3
- 125000001584 benzyloxycarbonyl group Chemical group C(=O)(OCC1=CC=CC=C1)* 0.000 claims description 3
- 239000004327 boric acid Substances 0.000 claims description 3
- 125000002147 dimethylamino group Chemical group [H]C([H])([H])N(*)C([H])([H])[H] 0.000 claims description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N ethyl acetate Substances CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 3
- PNGLEYLFMHGIQO-UHFFFAOYSA-M sodium;3-(n-ethyl-3-methoxyanilino)-2-hydroxypropane-1-sulfonate;dihydrate Chemical compound O.O.[Na+].[O-]S(=O)(=O)CC(O)CN(CC)C1=CC=CC(OC)=C1 PNGLEYLFMHGIQO-UHFFFAOYSA-M 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical class C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 claims 1
- 150000003851 azoles Chemical class 0.000 claims 1
- 125000005605 benzo group Chemical group 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 42
- 239000003814 drug Substances 0.000 abstract description 10
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 229940079593 drug Drugs 0.000 abstract description 5
- 238000004440 column chromatography Methods 0.000 abstract description 2
- 239000012535 impurity Substances 0.000 abstract description 2
- 230000003287 optical effect Effects 0.000 abstract description 2
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 46
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 40
- 238000005406 washing Methods 0.000 description 34
- 229940125898 compound 5 Drugs 0.000 description 31
- 238000004128 high performance liquid chromatography Methods 0.000 description 29
- 238000003756 stirring Methods 0.000 description 28
- 238000001914 filtration Methods 0.000 description 26
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 24
- 239000000243 solution Substances 0.000 description 23
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 21
- ONBQEOIKXPHGMB-VBSBHUPXSA-N 1-[2-[(2s,3r,4s,5r)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]oxy-4,6-dihydroxyphenyl]-3-(4-hydroxyphenyl)propan-1-one Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1OC1=CC(O)=CC(O)=C1C(=O)CCC1=CC=C(O)C=C1 ONBQEOIKXPHGMB-VBSBHUPXSA-N 0.000 description 20
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 20
- 229940126142 compound 16 Drugs 0.000 description 20
- 229940126214 compound 3 Drugs 0.000 description 20
- 238000007796 conventional method Methods 0.000 description 20
- AOSZTAHDEDLTLQ-AZKQZHLXSA-N (1S,2S,4R,8S,9S,11S,12R,13S,19S)-6-[(3-chlorophenyl)methyl]-12,19-difluoro-11-hydroxy-8-(2-hydroxyacetyl)-9,13-dimethyl-6-azapentacyclo[10.8.0.02,9.04,8.013,18]icosa-14,17-dien-16-one Chemical compound C([C@@H]1C[C@H]2[C@H]3[C@]([C@]4(C=CC(=O)C=C4[C@@H](F)C3)C)(F)[C@@H](O)C[C@@]2([C@@]1(C1)C(=O)CO)C)N1CC1=CC=CC(Cl)=C1 AOSZTAHDEDLTLQ-AZKQZHLXSA-N 0.000 description 18
- GLGNXYJARSMNGJ-VKTIVEEGSA-N (1s,2s,3r,4r)-3-[[5-chloro-2-[(1-ethyl-6-methoxy-2-oxo-4,5-dihydro-3h-1-benzazepin-7-yl)amino]pyrimidin-4-yl]amino]bicyclo[2.2.1]hept-5-ene-2-carboxamide Chemical compound CCN1C(=O)CCCC2=C(OC)C(NC=3N=C(C(=CN=3)Cl)N[C@H]3[C@H]([C@@]4([H])C[C@@]3(C=C4)[H])C(N)=O)=CC=C21 GLGNXYJARSMNGJ-VKTIVEEGSA-N 0.000 description 18
- 229940126657 Compound 17 Drugs 0.000 description 18
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 18
- 229940125758 compound 15 Drugs 0.000 description 18
- 238000006467 substitution reaction Methods 0.000 description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 150000001298 alcohols Chemical class 0.000 description 12
- 150000003863 ammonium salts Chemical class 0.000 description 12
- 150000008280 chlorinated hydrocarbons Chemical class 0.000 description 11
- 238000012805 post-processing Methods 0.000 description 11
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical group N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 10
- BZLVMXJERCGZMT-UHFFFAOYSA-N Methyl tert-butyl ether Chemical compound COC(C)(C)C BZLVMXJERCGZMT-UHFFFAOYSA-N 0.000 description 10
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 10
- 230000002140 halogenating effect Effects 0.000 description 10
- 238000010534 nucleophilic substitution reaction Methods 0.000 description 10
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 9
- 238000001514 detection method Methods 0.000 description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical class [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 8
- 235000011114 ammonium hydroxide Nutrition 0.000 description 8
- 239000007864 aqueous solution Substances 0.000 description 8
- 238000001035 drying Methods 0.000 description 8
- 150000008282 halocarbons Chemical class 0.000 description 8
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 8
- 238000012544 monitoring process Methods 0.000 description 7
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 6
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 6
- 239000005695 Ammonium acetate Substances 0.000 description 6
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 6
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 6
- 229940043376 ammonium acetate Drugs 0.000 description 6
- 235000019257 ammonium acetate Nutrition 0.000 description 6
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 6
- 239000012065 filter cake Substances 0.000 description 6
- NUJOXMJBOLGQSY-UHFFFAOYSA-N manganese dioxide Chemical compound O=[Mn]=O NUJOXMJBOLGQSY-UHFFFAOYSA-N 0.000 description 6
- 150000007530 organic bases Chemical class 0.000 description 6
- 239000012074 organic phase Substances 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- JQRYUMGHOUYJFW-UHFFFAOYSA-N pyridine;trihydrobromide Chemical compound [Br-].[Br-].[Br-].C1=CC=[NH+]C=C1.C1=CC=[NH+]C=C1.C1=CC=[NH+]C=C1 JQRYUMGHOUYJFW-UHFFFAOYSA-N 0.000 description 6
- JGFZNNIVVJXRND-UHFFFAOYSA-N N,N-Diisopropylethylamine (DIPEA) Chemical compound CCN(C(C)C)C(C)C JGFZNNIVVJXRND-UHFFFAOYSA-N 0.000 description 5
- 229910021529 ammonia Inorganic materials 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 239000000460 chlorine Substances 0.000 description 5
- 229910052801 chlorine Inorganic materials 0.000 description 5
- 238000001816 cooling Methods 0.000 description 5
- 239000000706 filtrate Substances 0.000 description 5
- 208000006454 hepatitis Diseases 0.000 description 5
- 235000011121 sodium hydroxide Nutrition 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
- 150000001335 aliphatic alkanes Chemical class 0.000 description 4
- 239000003054 catalyst Substances 0.000 description 4
- 238000002425 crystallisation Methods 0.000 description 4
- 230000008025 crystallization Effects 0.000 description 4
- 230000001186 cumulative effect Effects 0.000 description 4
- AMWRITDGCCNYAT-UHFFFAOYSA-L hydroxy(oxo)manganese;manganese Chemical compound [Mn].O[Mn]=O.O[Mn]=O AMWRITDGCCNYAT-UHFFFAOYSA-L 0.000 description 4
- 150000002825 nitriles Chemical class 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
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- 239000000047 product Substances 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 229910052708 sodium Inorganic materials 0.000 description 4
- LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Substances CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 description 3
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 3
- FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical compound Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 3
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 3
- 0 COC[C@](C*1*)C[C@]1C(OC*(C1C=C(COc2c3cc(CCCC4=O)c4c2)C3=CC1)=O)=O Chemical compound COC[C@](C*1*)C[C@]1C(OC*(C1C=C(COc2c3cc(CCCC4=O)c4c2)C3=CC1)=O)=O 0.000 description 3
- IMNFDUFMRHMDMM-UHFFFAOYSA-N N-Heptane Chemical compound CCCCCCC IMNFDUFMRHMDMM-UHFFFAOYSA-N 0.000 description 3
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- 239000001099 ammonium carbonate Substances 0.000 description 3
- 235000012501 ammonium carbonate Nutrition 0.000 description 3
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- 150000008040 ionic compounds Chemical class 0.000 description 3
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- 150000005837 radical ions Chemical class 0.000 description 3
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- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 3
- 235000014393 valine Nutrition 0.000 description 3
- VMHZXXPDUOVTHD-UHFFFAOYSA-N 2,3,4-trichloropyridine Chemical compound ClC1=CC=NC(Cl)=C1Cl VMHZXXPDUOVTHD-UHFFFAOYSA-N 0.000 description 2
- IMRWILPUOVGIMU-UHFFFAOYSA-N 2-bromopyridine Chemical compound BrC1=CC=CC=N1 IMRWILPUOVGIMU-UHFFFAOYSA-N 0.000 description 2
- YYROPELSRYBVMQ-UHFFFAOYSA-N 4-toluenesulfonyl chloride Chemical group CC1=CC=C(S(Cl)(=O)=O)C=C1 YYROPELSRYBVMQ-UHFFFAOYSA-N 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- 239000007821 HATU Substances 0.000 description 2
- AFVFQIVMOAPDHO-UHFFFAOYSA-N Methanesulfonic acid Chemical compound CS(O)(=O)=O AFVFQIVMOAPDHO-UHFFFAOYSA-N 0.000 description 2
- 239000012359 Methanesulfonyl chloride Substances 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 239000012317 TBTU Substances 0.000 description 2
- KPFBUSLHFFWMAI-HYRPPVSQSA-N [(8r,9s,10r,13s,14s,17r)-17-acetyl-6-formyl-3-methoxy-10,13-dimethyl-1,2,7,8,9,11,12,14,15,16-decahydrocyclopenta[a]phenanthren-17-yl] acetate Chemical compound C1C[C@@H]2[C@](CCC(OC)=C3)(C)C3=C(C=O)C[C@H]2[C@@H]2CC[C@](OC(C)=O)(C(C)=O)[C@]21C KPFBUSLHFFWMAI-HYRPPVSQSA-N 0.000 description 2
- GOPYZMJAIPBUGX-UHFFFAOYSA-N [O-2].[O-2].[Mn+4] Chemical class [O-2].[O-2].[Mn+4] GOPYZMJAIPBUGX-UHFFFAOYSA-N 0.000 description 2
- GPDHNZNLPKYHCN-DZOOLQPHSA-N [[(z)-(1-cyano-2-ethoxy-2-oxoethylidene)amino]oxy-morpholin-4-ylmethylidene]-dimethylazanium;hexafluorophosphate Chemical compound F[P-](F)(F)(F)(F)F.CCOC(=O)C(\C#N)=N/OC(=[N+](C)C)N1CCOCC1 GPDHNZNLPKYHCN-DZOOLQPHSA-N 0.000 description 2
- CLZISMQKJZCZDN-UHFFFAOYSA-N [benzotriazol-1-yloxy(dimethylamino)methylidene]-dimethylazanium Chemical compound C1=CC=C2N(OC(N(C)C)=[N+](C)C)N=NC2=C1 CLZISMQKJZCZDN-UHFFFAOYSA-N 0.000 description 2
- 239000008186 active pharmaceutical agent Substances 0.000 description 2
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 2
- 238000005660 chlorination reaction Methods 0.000 description 2
- NEHMKBQYUWJMIP-UHFFFAOYSA-N chloromethane Chemical class ClC NEHMKBQYUWJMIP-UHFFFAOYSA-N 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 229910052736 halogen Inorganic materials 0.000 description 2
- 125000005843 halogen group Chemical group 0.000 description 2
- 150000002367 halogens Chemical class 0.000 description 2
- NPZTUJOABDZTLV-UHFFFAOYSA-N hydroxybenzotriazole Substances O=C1C=CC=C2NNN=C12 NPZTUJOABDZTLV-UHFFFAOYSA-N 0.000 description 2
- ZFTKWAVDJBKFCS-UHFFFAOYSA-N iodine;pyridine Chemical class [I].C1=CC=NC=C1 ZFTKWAVDJBKFCS-UHFFFAOYSA-N 0.000 description 2
- QARBMVPHQWIHKH-UHFFFAOYSA-N methanesulfonyl chloride Chemical compound CS(Cl)(=O)=O QARBMVPHQWIHKH-UHFFFAOYSA-N 0.000 description 2
- TXOUDPQJASQYBZ-UHFFFAOYSA-N n,n'-dihexylmethanediimine Chemical compound CCCCCCN=C=NCCCCCC TXOUDPQJASQYBZ-UHFFFAOYSA-N 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- ITMCEJHCFYSIIV-UHFFFAOYSA-N triflic acid Chemical compound OS(=O)(=O)C(F)(F)F ITMCEJHCFYSIIV-UHFFFAOYSA-N 0.000 description 2
- IHQKEKMZKLDFDA-QWRGUYRKSA-N CCC[C@H](C)N([C@@H](C)C=C)C1CC1 Chemical compound CCC[C@H](C)N([C@@H](C)C=C)C1CC1 IHQKEKMZKLDFDA-QWRGUYRKSA-N 0.000 description 1
- SFMZBRIDMVOSKQ-UHFFFAOYSA-N CS(Cl(F)(F)F)(=O)=O Chemical compound CS(Cl(F)(F)F)(=O)=O SFMZBRIDMVOSKQ-UHFFFAOYSA-N 0.000 description 1
- IGBVFNPKSDQHKL-YFKPBYRVSA-N C[C@@H](CCC1)N1P Chemical compound C[C@@H](CCC1)N1P IGBVFNPKSDQHKL-YFKPBYRVSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- QUSNBJAOOMFDIB-UHFFFAOYSA-N Ethylamine Chemical compound CCN QUSNBJAOOMFDIB-UHFFFAOYSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- PCLIMKBDDGJMGD-UHFFFAOYSA-N N-bromosuccinimide Chemical class BrN1C(=O)CCC1=O PCLIMKBDDGJMGD-UHFFFAOYSA-N 0.000 description 1
- JRNVZBWKYDBUCA-UHFFFAOYSA-N N-chlorosuccinimide Chemical class ClN1C(=O)CCC1=O JRNVZBWKYDBUCA-UHFFFAOYSA-N 0.000 description 1
- MZBLZLWXUBZHSL-FZNJKFJKSA-N O=C([C@H]1N2C[C@H](OC3=NC4=CC(OC)=CC=C4N=C3C(F)(F)CCCC[C@@H]3C[C@H]3OC(=O)N[C@H](C2=O)C(C)(C)C)[C@H]1CC)N[C@]1(C(=O)NS(=O)(=O)C2(C)CC2)C[C@H]1C(F)F Chemical compound O=C([C@H]1N2C[C@H](OC3=NC4=CC(OC)=CC=C4N=C3C(F)(F)CCCC[C@@H]3C[C@H]3OC(=O)N[C@H](C2=O)C(C)(C)C)[C@H]1CC)N[C@]1(C(=O)NS(=O)(=O)C2(C)CC2)C[C@H]1C(F)F MZBLZLWXUBZHSL-FZNJKFJKSA-N 0.000 description 1
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 229910001870 ammonium persulfate Inorganic materials 0.000 description 1
- SRSXLGNVWSONIS-UHFFFAOYSA-N benzenesulfonic acid Chemical compound OS(=O)(=O)C1=CC=CC=C1 SRSXLGNVWSONIS-UHFFFAOYSA-N 0.000 description 1
- 229940092714 benzenesulfonic acid Drugs 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 229910052593 corundum Inorganic materials 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- AQEFLFZSWDEAIP-UHFFFAOYSA-N di-tert-butyl ether Chemical compound CC(C)(C)OC(C)(C)C AQEFLFZSWDEAIP-UHFFFAOYSA-N 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940039283 epclusa Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229940029169 harvoni Drugs 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical class [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 229940098779 methanesulfonic acid Drugs 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 239000003495 polar organic solvent Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- UGNWTBMOAKPKBL-UHFFFAOYSA-N tetrachloro-1,4-benzoquinone Chemical compound ClC1=C(Cl)C(=O)C(Cl)=C(Cl)C1=O UGNWTBMOAKPKBL-UHFFFAOYSA-N 0.000 description 1
- 125000002088 tosyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1C([H])([H])[H])S(*)(=O)=O 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 229940039243 velpatasvir 100 mg Drugs 0.000 description 1
- 229950004638 voxilaprevir Drugs 0.000 description 1
- 229910001845 yogo sapphire Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
- C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
- C07D491/04—Ortho-condensed systems
- C07D491/044—Ortho-condensed systems with only one oxygen atom as ring hetero atom in the oxygen-containing ring
- C07D491/052—Ortho-condensed systems with only one oxygen atom as ring hetero atom in the oxygen-containing ring the oxygen-containing ring being six-membered
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses GS5816 intermediates, preparation method and application.The invention provides the preparation method of compound 12, comprise the following steps：In organic solvent, under the conditions of condensing agent is existing, compound 13 and compound 9 are subjected to condensation reaction, compound 12 is made.The preparation method reaction condition of the present invention is gentle, safety simple to operate, without extraordinary purifier apparatus, avoid that operation, chiral isomer are used column chromatography in last handling process is easily controllable, Wei Patawei high incomes, obtained Wei Patawei chemistry and optical purity are prepared for intermediate with the compound 12 of the present invention and are more than 99.50%, all impurity are respectively less than 0.10%, can reach that bulk drug standard, cost are low, are suitable for industrialized production.
Description
Technical field
The present invention relates to GS5816 intermediates, preparation method and application.
Background technology
In June, 2016, FDA have approved the anti-hepatitis weight pound medicine Epclusa (Suo Feibuwei of the another money of Gilid Science Co.
400mg+Velpatasvir 100mg), the first anti-hepatitis medicine for being used to treat 1-6 type oligogene hypotypes.It is described as after rope
After Fei Buwei and Harvoni, the anti-hepatitis heavy bomb drugs of the third generation.
In October, 2016, Gilid Science Co. announced 4 three phase of whole world clinical study results, Suo Feibuwei, Wei Pata
Wei (Velpatasvir) and the three-in-one tablets of Voxilaprevir are notable to gene 1-6 type HCV infection patient's curative effects.With
Velpatasvir is the anti-hepatitis new drug of compound, and in July, 2017, FDA approval listings, medicine name Vosevi, Vosevi will quickly
As star's medicine in anti-hepatitis field, wide market.
The US20150361073 of Gilid Science Co. prepare patent disclose a kind of Wei Patawei (Velpatasvir or
GS5816 preparation method), its raw material fluorescence is weaker, and is difficult that control is chiral in building-up process, causes to be difficult to get to chiral purity
The higher intermediate of degree, then prepares API costs height, and difficulty is big.
Therefore, chiral isomer is easy to control in searching course of reaction, obtained product chiral purity is high, chemical purity
It is high, reach that bulk drug standard, total recovery are high, easy to operate, production cost is low, are suitable for the Wei Patawei of industrialized production
The preparation method of (Velpatasvir or GS5816) is current urgent problem.
The content of the invention
The technical problems to be solved by the invention be in order to overcome in the prior art Wei Patawei (Velpatasvir or
GS5816 preparation method), chiral isomer control is difficult in course of reaction, and obtained product chiral purity is poor, does not reach original
Expect medicine standard, total recovery is low, and production cost is high, the defects of being not suitable for industrialized production, and provides GS5816 intermediates, system
Preparation Method and application.The preparation method reaction condition of the present invention is gentle, and step is short, high income, and obtained product purity is high, can
Reach bulk drug (API) standard, production cost is low, is suitable for industrialized production.
The invention provides the preparation method of compound 12, and it comprises the following steps：In organic solvent, existing for condensing agent
Under the conditions of, compound 13 and compound 9 are subjected to condensation reaction, compound 12 is made；
The preparation method of described compound 12 can be the conventional method of such condensation reaction in this area, in of the invention
Particularly preferred following reaction method and condition：
In the preparation method of described compound 12, described organic solvent preferred amide class solvent；Described acid amides
The preferred N,N-dimethylformamide of class solvent (DMF).
In the preparation method of described compound 12, the preferred 1- ethyls of described condensing agent-(3- dimethylaminos third
Base) phosphinylidyne diimmonium salt hydrochlorate (EDCI), 2- (7- azos BTA)-N, N, N', N'- tetramethylurea hexafluorophosphoric acid esters
(HATU), BTA-N, N, N', N'- tetramethylurea hexafluorophosphoric acid ester (HBTU), O- BTAs-N, N, N', N'-
Tetramethylurea tetrafluoro boric acid ester (TBTU), BTA -1- three (dimethylamino)-trifluoro phosphate (BOP), chloro tripyrrole alkane
Ji Phosphonium hexafluorophosphate (PyClOP), hexafluorophosphoric acid BTA -1- bases-epoxide tripyrrole alkyl (PyBOP), the ring of N, N'- bis-
Hexyl carbodiimide (DCC), (2- oximidos-cyan-acetic ester)-N, N- Dimethyl-morpholin base urea hexafluorophosphoric acid esters (COMU),
2- oximes ethyl cyanoacetate (Oxyma) and propylphosphonic anhydride (T3P the one or more in).Described propylphosphonic anhydride (T3P) can be with
For conventional commercial propylphosphonic anhydride (T3P) reagent, such as mass concentration are 50% propylphosphonic anhydride (T3P) N, N- dimethyl formyl
Amine (DMF) solution, it is molten that described mass concentration refers to that the quality of propylphosphonic anhydride accounts for propylphosphonic anhydride DMF
The percentage of liquid gross mass.
In the preparation method of described compound 12, described condensing agent and the molar ratio of described compound 13 are excellent
Select 1~3, further preferred 1~1.5, such as 1.1.
In the preparation method of described compound 12, the molar ratio of described compound 9 and described compound 13
It is preferred that 1~3, further preferred 1~1.5, such as 1.1.
In the preparation method of described compound 12, preferably 0 DEG C~40 DEG C of the temperature of described condensation reaction, further
It is preferred that 10 DEG C~30 DEG C, such as 20 DEG C~25 DEG C.
In the preparation method of described compound 12, the process of described condensation reaction can be using normal in this area
Rule detection method (such as HPLC, TLC or NMR) is monitored, described as the terminal of reaction when typically being disappeared using compound 13
Preferably 1 hour~10 hours time of condensation reaction, further preferred 2 hours~8 hours, such as 5 hours~6 hours.
The preparation method of described compound 12 preferably uses following steps：0-10 DEG C, by compound 13 and organic solvent
The mixture of formation, it is added drop-wise in the mixture that compound 9, condensing agent and organic solvent are formed, carries out condensation reaction and obtain institute
The compound 12 stated.
The preparation method of described compound 12 preferably uses following post-processing step：After reaction terminates, cool, be added dropwise
Water, stir, filtering, washing obtains described compound 12.Described cooling is preferably dropped to 5 DEG C~10 DEG C of temperature.It is described
Stirring preferably 2 hours~3 hours time.
The preparation method of described compound 12 preferably further includes the preparation method of compound 13, and it includes following step
Suddenly：In organic solvent, compound 14 is carried out to the reaction of deaminizating protection group with acid, described compound 13 is made；
Wherein, described P is amino protecting group, preferably tertbutyloxycarbonyl, benzyloxycarbonyl group or p-toluenesulfonyl.
The preparation method of described compound 13 can be the routine of the reaction of such removing amino protecting group in this area
Method, particularly preferred following reaction method and condition in of the invention：
In the preparation method of described compound 13, the preferred esters solvent of described organic solvent；Described esters are molten
The preferred isopropyl acetate of agent.
In the preparation method of described compound 13, described sour preferred inorganic acid；The preferred chlorination of described inorganic acid
Hydrogen.Described hydrogen chloride can in gaseous form or its solution form use, the preferred hydrogen chloride of described hydrogen chloride solution
Isopropyl acetate solution.
In the preparation method of described compound 13, the described sour molar ratio preferably 1 with described compound 14
~10, further preferred 4~6, such as 5.
In the preparation method of described compound 13, preferably 40 DEG C of the temperature of the reaction of described removing amino protecting group
~100 DEG C, further preferred 50 DEG C~70 DEG C, such as 60 DEG C~65 DEG C.
In the preparation method of described compound 13, the process of the reaction of described removing amino protecting group can use
Common detection methods (such as HPLC, TLC or NMR) in this area are monitored, as reaction when typically being disappeared using compound 14
Terminal, preferably 1 hour~10 hours time of the reaction of described removing amino protecting group, further preferred 2 hours~8 is small
When, such as 2 hours~6 hours.
The preparation method of described compound 12 preferably further includes the preparation method of compound 14, and it includes following step
Suddenly：Under protective gas protection, in organic solvent, under the conditions of alkali is existing, compound 25 and compound 24 are subjected to condensation reaction,
Obtain described compound 14；
P is as defined above described.
The preparation method of described compound 14 can be the conventional method of such condensation reaction in this area, in of the invention
Particularly preferred following reaction method and condition：
In the preparation method of described compound 14, the preferred nitrogen of described protective gas and/or argon gas.
In the preparation method of described compound 14, described organic solvent preferred amide class solvent；Described acid amides
The preferred DMF of class solvent (DMF).
In the preparation method of described compound 14, the preferred inorganic base of described alkali；The preferred carbonic acid of described inorganic base
Potassium.
In the preparation method of described compound 14, described alkali and the molar ratio preferably 1 of described compound 25
~3, further preferred 1.00~1.50, such as 1.05.
In the preparation method of described compound 14, the molar ratio of described compound 24 and described compound 25
It is preferred that 1~3, further preferred 1.00~1.50, such as 1.05.
In the preparation method of described compound 14, preferably 10 DEG C~50 DEG C of the temperature of described condensation reaction, enter one
Preferably 20 DEG C~40 DEG C of step.
In the preparation method of described compound 14, the process of described condensation reaction can be using normal in this area
Rule detection method (such as HPLC, TLC or NMR) is monitored, as the terminal of reaction when typically being disappeared using described compound 25,
Preferably 1 hour~5 hours time of described condensation reaction, further preferred 2 hours~4 hours, such as 3 hours.
Present invention also offers the preparation method of compound 8, and it comprises the following steps：Preparation method as described above
Described compound 12 and then in organic solvent is made, compound 12 and halogenating agent are subjected to substitution reaction and obtain institute
The compound 8 stated；
Wherein, L represents leaving group, such as p-toluenesulfonyl (Tos), mesyl (- OMs), trifyl
(-OSO2CF3), Br, Cl or I；Described halogenating agent is paratoluensulfonyl chloride, mesyl chloride, trifluoromethanesulfchloride chloride, tribromo pyrrole
Pyridine, trichloropyridine or three iodine pyridines.
The preparation method of described compound 8 can be the conventional method of such substitution reaction in this area, in of the invention
Particularly preferred following reaction condition：
In the preparation method of described compound 8, the preferred halogenated hydrocarbon solvent of described organic solvent；Described halo
The preferred chlorinated hydrocarbon solvent of varsol；The preferred dichloromethane of described chlorinated hydrocarbon solvent.
In the preparation method of described compound 8, described halogenating agent and the molar ratio of described compound 12
It is preferred that 1~3, further preferred 1.1~1.5, such as 1.1.
In the preparation method of described compound 8, when doing halogenating agent using pyridinium tribromide, described pyridinium tribromide
Can be conventional commercial pyridinium tribromide reagent in this area, such as pyridinium tribromide methanol solution.
In the preparation method 2 of described compound 8, preferably 0 DEG C~40 DEG C of the temperature of described substitution reaction, further
It is preferred that 10 DEG C~15 DEG C.
In the preparation method of described compound 8, the process of described substitution reaction can be using normal in this area
Rule detection method (such as HPLC, TLC or NMR) is monitored, and is typically disappeared with compound 12 for reaction end, described substitution
Preferably 1 hour~10 hours time of reaction, further preferred 2 hours~5 hours, such as 3 hours.
The preparation method of described compound 8, it is preferred to use following steps：0 DEG C~5 DEG C, by being added dropwise to of halogenating agent
In the mixed liquor that compound 12 is formed with organic solvent, carry out substitution reaction and obtain described compound 8.
The preparation method of described compound 8, it is preferred to use following post-processing step：After reaction terminates, filter, wash,
It is dried to obtain described compound 8.Described filtering, washing, drying can use the conventional method of the generic operation in this area.
Described washing preferably uses alcohols solvent, the preferred methanol of described alcohols solvent.
Present invention also offers the preparation method of compound 6, and it comprises the following steps：Preparation method as described above
Described compound 8 is made and then under protective gas protection, in organic solvent, under the conditions of alkali is existing, by compound 8
Nucleophilic substitution is carried out with compound 7, obtains described compound 6；
Wherein, P is as defined above described；L represent leaving group, such as p-toluenesulfonyl (Tos), mesyl (-
OMs), trifyl (- OSO2CF3), Br, Cl or I.
The preparation method of described compound 6 can be the conventional method of such nucleophilic substitution in this area, this hair
Bright particularly preferably following reaction method and condition：
In the preparation method of described compound 6, the preferred nitrogen of described protective gas and/or argon gas.
In the preparation method of described compound 6, described organic solvent preferred amide class solvent, nitrile solvents, ether
One or more in class solvent and halogenated hydrocarbon solvent.The preferred DMF of described amide solvent (DMF).
The preferred acetonitrile of described nitrile solvents.The preferred tetrahydrofuran of described ether solvent.The preferred chloro of described halogenated hydrocarbon solvent
Varsol；The preferred dichloromethane of described chlorinated hydrocarbon solvent.
In the preparation method of described compound 6, described alkali can be inorganic base or organic base；Described inorganic base
It is preferred that potassium carbonate and/or sodium carbonate.The preferred triethylamine of described organic base and/or N, N- diisopropyl ethyl amine.
In the preparation method of described compound 6, the molar ratio preferably 1 of described alkali and described compound 8~
3, further preferred 1.00~1.20, such as 1.05.
In the preparation method of described compound 6, the molar ratio of described compound 7 and described compound 8 is excellent
Select 1~3, further preferred 1.00~1.20, such as 1.05.
In the preparation method of described compound 6, preferably 10 DEG C~50 DEG C of the temperature of described nucleophilic substitution, enter
Preferably 15 DEG C~45 DEG C of one step, such as 20 DEG C~40 DEG C.
In the preparation method of described compound 6, the process of described nucleophilic substitution can be used in this area
Routine monitoring method (such as TLC, HPLC or NMR) be monitored, as the terminal of reaction, institute when typically being disappeared using compound 8
Preferably 5 hours~24 hours time for the nucleophilic substitution stated, further preferred 10 hours~20 hours, such as 16 hours.
The preparation method of described compound 6, following post-processing step can be used：Reaction terminate after, cooling, plus water,
Stir, filter, washing, being dried to obtain compound 6 after purification.Described cooling is preferably dropped to temperature as 5 DEG C~10 DEG C.
The time of described stirring can be 2 hours~3 hours.Described washing and drying can use the generic operation in this area
Conventional method.Described washing can use water.Described drying can use vacuum drying.
Present invention also offers the preparation method of compound 17, and it comprises the following steps：Preparation side as described above
Method is made described compound 12 and then under protective gas protection, and in organic solvent, compound 12 is contracted with ammonium salt
Close the compound 17 that reaction is made described；
The preparation method of described compound 17 can be the conventional method of such condensation reaction in this area, and the present invention is special
Not not preferably following reaction method and condition：
In the preparation method of described compound 17, the preferred nitrogen of described protective gas and/or argon gas.
In the preparation method of described compound 17, described organic solvent preferred aromatic hydrocarbons class solvent；Described aromatic hydrocarbons
The preferred toluene of class solvent.
In the preparation method of described compound 17, described ammonium salt is ammonia with acid reaction generation by ammonium ion and acid
The ionic compound that radical ion is formed.In the preferred ammonium acetate of described ammonium salt, ammonium chloride, ammonium nitrate, ammonium carbonate and ammonium sulfate
It is one or more.
In the preparation method of described compound 17, described ammonium salt and the molar ratio of described compound 12 are preferred
1~40, further preferred 15~25, such as 20.
In the preparation method of described compound 17, preferably 50 DEG C~120 DEG C of the temperature of described condensation reaction, such as
80 DEG C~100 DEG C.
In the preparation method of described compound 17, the process of described condensation reaction can be using normal in this area
Rule detection method (such as TLC, HPLC or NMR) is monitored, as the end of reaction when typically being disappeared using compound 12 and intermediate state
Point, preferably 5 hours~24 hours time of described condensation reaction, further preferred 15 hours~22 hours, such as 20 hours.
The preparation method of described compound 17, following post-processing step can be used：After reaction terminates, it is molten to add alcohols
Agent, washing, removing solvent obtain compound 17 after purification.The preferred n-butanol of described alcohols solvent.Described washing
The conventional method of the generic operation in this area can be used with solvent is removed.Described washing preferably uses water and/or saturation chlorine
Change sodium water solution washing.Described removing solvent is preferably by the way of being evaporated under reduced pressure.
Present invention also offers the preparation method of compound 16, and it comprises the following steps：Preparation side as described above
Described compound 17 and then in organic solvent is made in method, and compound 17 and halogenating agent are carried out into substitution reaction, are made
Described compound 16；
Wherein, L is as defined above described；Described halogenating agent is paratoluensulfonyl chloride, mesyl chloride, trifluoro methylsulfonyl
Chlorine, pyridinium tribromide, trichloropyridine or three iodine pyridines.
The preparation method of described compound 16 can be the conventional method of such substitution reaction in this area, in of the invention
Particularly preferred following reaction condition：
In the preparation method of described compound 16, the preferred halogenated hydrocarbon solvent of described organic solvent；Described halogen
For the preferred chlorinated hydrocarbon solvent of varsol；The preferred dichloromethane of described chlorinated hydrocarbon solvent.
In the preparation method of described compound 16, described halogenating agent and the molar ratio of described compound 17
It is preferred that 1~3, further preferred 1.1~1.5, such as 1.1.
In the preparation method of described compound 16, when doing halogenating agent using pyridinium tribromide, described tribromo pyrrole
Pyridine can be conventional commercial pyridinium tribromide reagent in this area, such as pyridinium tribromide methanol solution.
In the preparation method of described compound 16, preferably 0 DEG C~40 DEG C of the temperature of described substitution reaction, further
It is preferred that 10 DEG C~15 DEG C.
In the preparation method of described compound 16, the process of described substitution reaction can be using normal in this area
Rule detection method (such as HPLC, TLC or NMR) is monitored, and is typically disappeared with compound 17 for reaction end, described substitution
Preferably 1 hour~10 hours time of reaction, further preferred 2 hours~5 hours, such as 3 hours.
The preparation method of described compound 16, it is preferred to use following steps：0 DEG C~5 DEG C, by being added dropwise to of halogenating agent
In the mixed liquor that compound 17 is formed with organic solvent, carry out substitution reaction and obtain described compound 16.
In the preparation method of described compound 16, it is preferred to use following post-processing step：After reaction terminates, filter, wash
Wash, be dried to obtain described compound 16.Described filtering, washing, drying can use the routine of the generic operation in this area
Method.Described washing preferably uses alcohols solvent, the preferred methanol of described alcohols solvent.
Present invention also offers the preparation method of compound 15, and it comprises the following steps：Preparation side as described above
Method is made described compound 16 and then under protective gas protection, in organic solvent, under the conditions of alkali is existing, by chemical combination
Thing 16 carries out the obtained described compound 15 of nucleophilic substitution with compound 7；
Wherein, P is as defined above described；L represent leaving group, such as p-toluenesulfonyl (Tos), mesyl (-
OMs), trifyl (- OSO2CF3), Br, Cl or I.
The preparation method of described compound 15 can be the conventional method of such nucleophilic substitution in this area, this hair
Bright particularly preferably following reaction condition：
In the preparation method of described compound 15, the preferred nitrogen of described protective gas and/or argon gas.
In the preparation method of described compound 15, described organic solvent preferred amide class solvent, described acid amides
The preferred DMF of class solvent (DMF).
In the preparation method of described compound 15, the preferred inorganic base of described alkali, the preferred carbonic acid of described inorganic base
Potassium.
In the preparation method of described compound 15, described alkali and the molar ratio of described compound 16 are preferred
1.00~3.00, further preferred 1.00~1.50, such as 1.05.
In the preparation method of described compound 15, the molar ratio of described compound 7 and described compound 16
It is preferred that 1.00~3.00, further preferred 1.00~1.50, such as 1.05.
In the preparation method of described compound 15, preferably 10 DEG C~60 DEG C of the temperature of described nucleophilic substitution,
Further preferred 20 DEG C~40 DEG C.
In the preparation method of described compound 15, the process of described nucleophilic substitution can be used in this area
Routine monitoring method (such as HPLC, TLC or NMR) be monitored, typically disappeared with compound 16 for reaction end, it is described
Preferably 10 hours~30 hours time of nucleophilic substitution, further preferred 12 hours~20 hours, such as 16 hours.
In the preparation method of described compound 15, it is preferred to use following post-processing step：After reaction terminates, cooling,
Add water, stir, filtering, be dried to obtain compound 15.Described cooling is preferably dropped to -5 DEG C of temperature~10 DEG C, such as 5 DEG C~10
℃.Described plus water is preferably added dropwise, and the speed of dropwise addition is defined by system temperature no more than 10 DEG C.Described stirring, filtering, drying
The conventional method of the generic operation in this area can be used.
Present invention also offers the preparation method of compound 5, and it is method A or method B；
Method A it comprises the following steps：Preparation method as described above be made described compound 6 and then
Under protective gas protection, in organic solvent, compound 6 and ammonium salt are subjected to condensation reaction and obtain described compound 5；
P is defined as above described；
Method B comprises the following steps：Preparation method as described above is made described compound 15 and then protected
Protect under gas shield, in organic solvent, compound 15 and ammonium salt are subjected to condensation reaction and obtain described compound 5；
P is defined as above described.
The preparation method A of described compound 5 can be the conventional method of such condensation reaction in this area, and the present invention is special
Not not preferably following reaction method and condition：
In the preparation method A of described compound 5, the preferred nitrogen of described protective gas and/or argon gas.
In the preparation method A of described compound 5, described organic solvent preferred aromatic hydrocarbons class solvent；Described aromatic hydrocarbons
The preferred toluene of class solvent.
In the preparation method A of described compound 5, described ammonium salt is ammonia with acid reaction generation by ammonium ion and acid
The ionic compound that radical ion is formed.In the preferred ammonium acetate of described ammonium salt, ammonium chloride, ammonium nitrate, ammonium carbonate and ammonium sulfate
It is one or more.
In the preparation method A of described compound 5, described ammonium salt and the molar ratio preferably 1 of described compound 6
~40, further preferred 15~25, such as 20.
In the preparation method A of described compound 5, preferably 50 DEG C~120 DEG C of the temperature of described condensation reaction, enter one
Preferably 60 DEG C~110 DEG C of step, such as 80 DEG C~100 DEG C.
In the preparation method A of described compound 5, the process of described condensation reaction can be using normal in this area
Rule monitoring method (such as TLC, HPLC or NMR) is monitored, as the end of reaction when typically being disappeared using compound 6 and intermediate state
Point, preferably 5 hours~24 hours time of described condensation reaction, further preferred 15 hours~22 hours, such as 20 hours.
The preparation method A of described compound 5, it is preferred to use following post-processing step：After reaction terminates, it is molten to add alcohols
Agent, washing, removing solvent obtain compound 5 after purification.Described alcohols solvent can be n-butanol.Described washing
The conventional method of the generic operation in this area can be used with solvent is removed.Described washing preferably uses water and/or saturation chlorine
Change sodium water solution washing.Described removing solvent is preferably by the way of being evaporated under reduced pressure.
The preparation method B of described compound 5 can be the conventional method of such condensation reaction in this area, and the present invention is special
Not not preferably following reaction method and condition：
In the preparation method B of described compound 5, the preferred nitrogen of described protective gas and/or argon gas.
In the preparation method B of described compound 5, described organic solvent preferred aromatic hydrocarbons class solvent；Described aromatic hydrocarbons
The preferred toluene of class solvent.
In the preparation method B of described compound 5, described ammonium salt is ammonia with acid reaction generation by ammonium ion and acid
The ionic compound that radical ion is formed.In the preferred ammonium acetate of described ammonium salt, ammonium chloride, ammonium nitrate, ammonium carbonate and ammonium sulfate
It is one or more.
In the preparation method B of described compound 5, described ammonium salt and the molar ratio of described compound 15 are preferred
1~40, further preferred 15~25, such as 20.
In the preparation method B of described compound 5, preferably 50 DEG C~120 DEG C of the temperature of described condensation reaction, enter one
Preferably 60 DEG C~110 DEG C of step, such as 80 DEG C~100 DEG C.
In the preparation method B of described compound 5, the process of described condensation reaction can be using normal in this area
Rule monitoring method (such as TLC, HPLC or NMR) is monitored, as the end of reaction when typically being disappeared using compound 15 and intermediate state
Point, preferably 5 hours~24 hours time of described condensation reaction, further preferred 15 hours~22 hours, such as 20 hours.
The preparation method B of described compound 5, following post-processing step can be used：After reaction terminates, it is molten to add alcohols
Agent, washing, removing solvent obtain compound 5 after purification.The preferred n-butanol of described alcohols solvent.Described washing and
The conventional method of the generic operation in this area can be used by removing solvent.Described washing preferably uses water and/or saturation chlorination
Sodium water solution washs.Described removing solvent is preferably by the way of being evaporated under reduced pressure.
Present invention also offers the preparation method of compound 4, and it comprises the following steps：Preparation method as described above
Described compound 5 is made and then under protective gas protection, in organic solvent, compound 5 is aoxidized with oxidant
Reaction, obtain described compound 4；
P is defined as above described.
The preparation method of described compound 4 can be the conventional method of such oxidation reaction in this area, and the present invention is special
Not not preferably following reaction method and condition：
In the preparation method of described compound 4, the preferred nitrogen of described protective gas and/or argon gas.
In the preparation method of described compound 4, the preferred halogenated hydrocarbon solvent of described organic solvent；Described halo
The preferred chlorinated hydrocarbon solvent of varsol；The preferred dichloromethane of described chlorinated hydrocarbon solvent.
In the preparation method of described compound 4, the preferred manganese dioxide of described oxidant, DDQ
(DDQ), chloranil, N- chlorosuccinimides (NCS), N- bromo-succinimides (NBS) and Pd/Al2O3/O2In one kind or
It is a variety of.The activated manganese dioxide of the preferred now-making-now-using of described manganese dioxide.The preparation method of described manganese dioxide can adopt
With the customary preparation methods in this area.
In the preparation method of described compound 4, described oxidant and the molar ratio of described compound 5 are preferred
1~20, further preferred 5~15, such as 10.
In the preparation method of described compound 4, preferably 0 DEG C~40 DEG C of the temperature of described oxidation reaction, further
It is preferred that 1 DEG C~20 DEG C, such as 5 DEG C~10 DEG C.
In the preparation method of described compound 4, the process of described oxidation reaction can be using normal in this area
Rule detection method (such as TLC, HPLC or NMR) is monitored, described as the terminal of reaction when typically being disappeared using compound 5
Preferably 5 hours~24 hours time of oxidation reaction, such as 10 hours~15 hours.
The preparation method of described compound 4, it is preferred to use following post-processing step：After reaction terminates, add in reaction solution
Enter diatomite, stirring, filtering, remove solvent, then add ether solvent and inorganic base aqueous solution, flow back, filtering, remove part
Solvent, crystal seed is added, crystallization, obtains compound 4.Described stirring, filtering and removing solvent can be used in this area
The conventional method of the generic operation.Described ether solvent can be methyl tertiary butyl ether(MTBE).The preferred sodium hydroxide of described inorganic base
Or potassium hydroxide.The mass concentration of described inorganic base aqueous solution preferably 5%~40%, further preferred 5%~15%, example
Such as 10%, described mass concentration refers to that the quality of inorganic base accounts for the percentage of inorganic base aqueous solution gross mass.Described " removes
Partial solvent " refers to that the ratio of the solvent volume and filtrate solvent cumulative volume removed is 0.2~0.7.The temperature of described crystallization
It is preferred that 0 DEG C~20 DEG C.After described crystallization preferably removes partial solvent, not benign solvent crystallization is added；Described is not benign molten
The preferred alkane solvents of agent, the preferred normal heptane of described alkane solvents.
Present invention also offers the preparation method of compound 3, and it comprises the following steps：Preparation method as described above
Compound 4 described in being made and then the reaction by compound 4 and acid progress deaminizating protection group, obtain described compound 3
；
Wherein, P is amino protecting group, such as tertbutyloxycarbonyl (Boc), benzyloxycarbonyl group (CBz) or p-toluenesulfonyl
(Tosyl)。
The preparation method of described compound 3 can be the conventional method that such deaminizating protection group is reacted in this area,
Following reaction method specifically preferred according to the invention and condition：
The preparation method of described compound 3 can be carried out in a solvent or under the conditions of solvent-free；When entering in a solvent
During row, the one or more in the preferred alcohols solvent of described solvent, halogenated hydrocarbon solvent and ether solvent.Described alcohols is molten
The preferred methanol of agent.The preferred chlorinated hydrocarbon solvent of described halogenated hydrocarbon solvent；The preferred dichloromethane of described chlorinated hydrocarbon solvent.
The preferred tetrahydrofuran of described ether solvent.
In the preparation method of described compound 3, described acid can be organic acid or inorganic acid.Described organic acid
It is preferred that the one or more in trifluoromethanesulfonic acid, benzene sulfonic acid and methanesulfonic acid.The preferred hydrochloric acid of described inorganic acid and/or sulfuric acid.Institute
The hydrochloric acid stated can be conventional commercial hydrochloric acid reagent, and the mass concentration of described hydrochloric acid can be 20%~37%, described matter
Amount concentration refers to that the quality of hydrogen chloride accounts for the percentage of hydrochloric acid solution gross mass.
In the preparation method of described compound 3, the described sour and molar ratio preferably 1 of described compound 4~
20, further preferred 5~15, such as 10.
In the preparation method of described compound 3, preferably 40 DEG C of the temperature of the reaction of described deaminizating protection group~
70 DEG C, such as 60 DEG C~65 DEG C.
In the preparation method of described compound 3, the process of the reaction of described deaminizating protection group can use this
Common detection methods (such as TLC, HPLC or NMR) in field are monitored, as the end of reaction when typically being disappeared using compound 4
Point, preferably 1 hour~10 hours time of the reaction of described deaminizating protection group, such as 2 hours~6 hours.
The preparation method of described compound 3, it is preferred to use following post-processing step：After reaction terminates, solvent is added, is stirred
Mix, filter, it be 11-12 that solid, which is suspended in organic solvent and adjusts pH, and washing, removing solvent obtain compound 3 after purification i.e.
Can.The preferred ether solvent of described solvent, the preferred methyl tertiary butyl ether(MTBE) of described ether solvent.The preferred halogen of described organic solvent
For varsol；The preferred chlorinated hydrocarbon solvent of described halogenated hydrocarbon solvent；The preferred dichloromethane of described chlorinated hydrocarbon solvent.
Described regulation pH preferably uses organic base；The preferred ammoniacal liquor of described organic base.Described ammoniacal liquor can be conventional in this area
Commercially available ammoniacal liquor reagent, the mass concentration of described ammoniacal liquor can be 5%~25%, and described mass concentration refers to the quality of ammonia
Account for the percentage of ammonia spirit gross mass.Described washing and removing solvent can use the routine side of the generic operation in this area
Method.Described washing is preferably washed using water and/or saturated sodium-chloride water solution.Described removing solvent is preferably steamed using decompression
The mode evaporated.
Present invention also offers Wei Patawei 1 (GS5816) preparation method, and it comprises the following steps：As described above
Preparation method described compound 3 and then in organic solvent is made, under the conditions of alkali, catalyst and condensing agent are existing,
Compound 3 and MOC-L- valines are subjected to condensation reaction, obtain Wei Patawei 1；
Described Wei Patawei 1 preparation method can be the conventional method of such condensation reaction in this area, of the invention
Particularly preferred following reaction method and condition：
In described Wei Patawei 1 preparation method, the preferred polar organic solvent of described organic solvent；Described pole
One or more in the preferred nitrile solvents of property organic solvent, amide solvent and ether solvent.Described nitrile solvents are preferred
Acetonitrile.The preferred N,N-dimethylformamide of described amide solvent (DMF).The preferred tetrahydrofuran of described ether solvent
(THF)。
In described Wei Patawei 1 preparation method, the preferred I-hydroxybenzotriazole of described catalyst (HOBt).
In described Wei Patawei 1 preparation method, described catalyst and the molar ratio of described compound 3 are excellent
Select 1~5, further preferred 2~4, such as 2.6.
In described Wei Patawei 1 preparation method, the preferred 1- ethyls of described condensing agent-(3- dimethylaminos third
Base) phosphinylidyne diimmonium salt hydrochlorate (EDCI), 2- (7- azos BTA)-N, N, N', N'- tetramethylurea hexafluorophosphoric acid esters
(HATU), BTA-N, N, N', N'- tetramethylurea hexafluorophosphoric acid ester (HBTU), O- BTAs-N, N, N', N'-
Tetramethylurea tetrafluoro boric acid ester (TBTU), BTA -1- three (dimethylamino)-trifluoro phosphate (BOP), chloro tripyrrole alkane
Ji Phosphonium hexafluorophosphate (PyClOP), hexafluorophosphoric acid BTA -1- bases-epoxide tripyrrole alkyl (PyBOP), the ring of N, N'- bis-
Hexyl carbodiimide (DCC), (2- oximidos-cyan-acetic ester)-N, N- Dimethyl-morpholin base urea hexafluorophosphoric acid esters (COMU),
2- oximes ethyl cyanoacetate (Oxyma) and propylphosphonic anhydride (T3P the one or more in).
In described Wei Patawei 1 preparation method, described condensing agent and the molar ratio of described compound 3 are excellent
Select 1~5, further preferred 2~4, such as 2.5.
In described Wei Patawei 1 preparation method, the preferred organic base of described alkali；Described organic base preferred N, N-
Diisopropylethylamine (DIPEA) and/or triethylamine.
In described Wei Patawei 1 preparation method, described alkali and the molar ratio preferably 1 of described compound 3
~5, further preferred 2~4, such as 3.5.
In described Wei Patawei 1 preparation method, preferably -5 DEG C~30 DEG C of the temperature of described condensation reaction, enter one
Preferably 0 DEG C~20 DEG C of step, such as 0 DEG C~15 DEG C.
In described Wei Patawei 1 preparation method, the process of described condensation reaction can be used in this area
Routine monitoring method (such as TLC, HPLC or NMR) is monitored, described as the terminal of reaction when typically being disappeared using compound 3
Condensation reaction preferably 5 hours~24 hours time, further preferred 10 hours~20 hours, such as 12 hours~16 is small
When.
Described Wei Patawei 1 preparation method, it is preferred to use following reactions steps：Compound 3 and alkali are sequentially added
In the mixture that MOC-L- valines, catalyst, condensing agent and organic solvent are formed, condensation reaction is carried out, obtains Wei Patawei 1
.
Described Wei Patawei 1 preparation method, following post-processing step can be used：After reaction terminates, extraction, mistake
Filter, washing, removing solvent obtain Wei Patawei 1 after purification.Described extraction, filtering, washing and remove solvent can be with
Using the conventional method of the generic operation in this area.The solvent that described extraction uses can be isopropyl acetate and water.It is described
Washing can use sodium hydrate aqueous solution, saturated sodium-chloride water solution and water washing successively.Described sodium hydroxide is water-soluble
The molar concentration of liquid can be 0.5mol/L (i.e. 0.5N)；Described molar concentration refers to the mole and hydroxide of sodium hydroxide
The ratio of sodium water solution cumulative volume.Described removing solvent can be by the way of being evaporated under reduced pressure.
Described Wei Patawei 1 preparation method can use following route
Route 1：
Route 2：
Present invention also offers the and of midbody compound 3,4,5,6,8,12,13,14,15,16 for preparing Wei Patawei 1
17, its structural formula is as follows：
Present invention also offers the described midbody compound 3 for preparing Wei Patawei, 4,5,6,8,12,13,14,15,
16 and 17 preparation method, its reactions steps are same as above.
Without prejudice to the field on the basis of common sense, above-mentioned each optimum condition, can be combined, and it is each preferably to produce the present invention
Example.
Agents useful for same and raw material of the present invention are commercially available.
In the present invention, described room temperature refers to environment temperature, is 10 DEG C~35 DEG C.
The positive effect of the present invention is：The preparation method reaction condition of the present invention is gentle, safety simple to operate, nothing
Extraordinary purifier apparatus is needed, avoids operation, high income (reaching 67%), obtained dimension pa is used column chromatography in last handling process
His Wei Chundu is high, and (chemistry and optical purity are all higher than 99.50%, and all impurity are respectively less than 0.10%, can reach bulk drug mark
It is accurate), cost is low, is suitable for industrialized production.
Embodiment
The present invention is further illustrated below by the mode of embodiment, but does not therefore limit the present invention to described reality
Apply among a scope.The experimental method of unreceipted actual conditions in the following example, conventionally and condition, or according to business
Product specification selects.
The preparation (P Boc) of the compound 14 of embodiment 1
Compound 25 (1.96kg, 5.29mol, 1.0eq) and compound 24 (1.37kg, 1.05eq) are dissolved in N, N- dimethyl
In formamide (DMF), potassium carbonate (730.8g, 1.05eq) is added, nitrogen vacuum displacement three times, it is small to be heated to 20-40 DEG C of stirring 3
When.TLC shows that compound 25 is reacted completely.5-10 DEG C is down to, water is instilled, 5-10 DEG C, stirs 2-3 hours, filtering, filter cake water
Washing, dry, obtain 2.85kg compounds 14, yield 98.0%, HPLC purity 98.56%.
The preparation (P Boc, i.e. tertbutyloxycarbonyl) of the compound 13 of embodiment 2
Compound 14 (2.85kg, 5.18mol, 1eq) is dissolved in isopropyl acetate, adds isopropyl acetate/hydrogen chloride
(1.33L, 5eq), it is heated to reflux (60 DEG C~65 DEG C), reacts 2-6 hours, TLC shows complete reaction, is down to room temperature, slowly drop
Enter methylate tertbutyl ether, rear 0-10 DEG C of stirring 2-5 hour, filtering is added dropwise, solid is suspended in dichloromethane, added full
And sodium acid carbonate, stir 1 hour, filtering, organic phase washed with water, saturated sodium-chloride washing, concentration is dry, obtains 2.21kg compounds
13, yield 95.0%, HPLC purity 98.86%.
The preparation (method 2, L Br) of the compound 8 of embodiment 3
Compound 12 (3.02kg, 4.72mol, 1.0eq) is dissolved in dichloromethane, under ice bath (0 DEG C~5 DEG C), adds three
The methanol solution of bromopyridine (1.66kg, 1.1eq), 10-15 DEG C is stirred 3 hours, and TLC display reactions are complete.Filtering, filter cake is with two
Chloromethanes (DCM) elutes, and methanol elution, 50 DEG C of drying obtain 3.26kg bright yellow solids compound 8, yield 96.0%, HPLC
Purity 98.64%.
The preparation of the compound 12 of embodiment 4
Compound 13 (2.21kg, 4.92mol, 1.0eq) is dissolved in stand-by in N,N-dimethylformamide (DMF).
By compound 9 (1.3kg, 1.1eq), mass concentration is 50% propylphosphonic anhydride (T3P) N,N-dimethylformamide
(DMF) (described mass concentration refers to that the quality of propylphosphonic anhydride accounts for propylphosphonic anhydride N, N- bis- to solution (5.02kg, 1.1eq)
The percentage of NMF solution gross mass), it is dissolved in DMF, is down to 0-10 DEG C, instills the solution of above-mentioned configuration, 20 DEG C~
25 DEG C of stirring 5-6 hours, TLC show that compound 13 is reacted completely, are down to 5-10 DEG C, instill water, 5-10 DEG C, stir 2-3 hours,
Filtering, filter cake are washed with water, and dry, obtain 3.06kg compounds 12, yield 97.0%, HPLC purity 98.71%.
The preparation of the compound 17 of embodiment 5
Compound 12 (3.06kg, 4.77mol, 1.0eq) is dissolved in toluene, adds ammonium acetate (7.40kg, 20eq), nitrogen is true
Empty substitution three times, is heated to 80-100 DEG C (outer temperature) and stirred 20 hours.TLC shows complete reaction, is cooled to room temperature (20 DEG C~25
℃).N-butanol, organic phase washed with water are added, saturated common salt water washing is concentrated to give 2.93kg compounds 17, yield 99.0%,
HPLC purity 98.95%.
Preparation (the P Boc of the compound 6 of embodiment 6；L is Br)
Compound 8 (1.96kg, 2.73mol, 1.0eq) and compound 7 (659g, 1.05eq) are dissolved in N, N- dimethyl formyls
In amine (DMF), potassium carbonate (376.3g, 1.05eq) is added, nitrogen vacuum displacement three times, is heated to 20-40 DEG C and stirred 16 hours.
TLC shows that compound 8 is reacted completely.5-10 DEG C is down to, water is instilled, 5-10 DEG C, stirs 2-3 hours, filtering, filter cake is washed with water
Wash, dry, obtain 2.25kg compounds 6, yield 95.0%, HPLC purity 98.46%.
The preparation (L Br) of the compound 16 of embodiment 7
Compound 17 (2.93kg, 4.72mol, 1.0eq) is dissolved in dichloromethane, under ice bath (0 DEG C~5 DEG C), adds three
The methanol solution of bromopyridine (1.66kg, 1.1eq), 10-15 DEG C is stirred 3 hours, and TLC display reactions are complete.Filtering, filter cake is with two
Chloromethanes (DCM) elutes, and methanol elution, 50 DEG C of drying obtain 3.19kg bright yellow solids compound 16, yield 96.7%, HPLC
Purity 98.92%.
Preparation (the P Boc of the compound 15 of embodiment 8；L is Br)
Compound 16 (3.19kg, 4.71mol, 1.0eq) and compound 7 (1.13kg, 1.05eq) are dissolved in N, N- dimethyl
In formamide (DMF), potassium carbonate (682.3g, 1.05eq) is added, nitrogen vacuum displacement three times, is heated to 20-40 DEG C of stirring 16
Hour.TLC shows that compound 16 is reacted completely.5-10 DEG C is down to, water is instilled, 5-10 DEG C, stirs 2-3 hours, filtering, filter cake is used
Water washing, dry, obtain 3.79kg compounds 15, yield 95.0%, HPLC purity 98.57%.
The preparation (P Boc) of the compound 5 of embodiment 9
Compound 6 (2.25kg, 2.59mol, 1.0eq) is dissolved in toluene, adds ammonium acetate (4.01kg, 20eq), nitrogen is true
Empty substitution three times, is heated to 80-100 DEG C (outer temperature) and stirred 20 hours.TLC shows complete reaction, is cooled to 20 DEG C~25 DEG C, adds
Enter n-butanol, organic phase washed with water, saturated common salt water washing, be concentrated to give 2.13kg compounds 5, yield 99.0%, HPLC is pure
Degree 98.87%.
The preparation (P Boc) of the compound 5 of embodiment 10
Compound 15 (3.79kg, 4.47mol, 1.0eq) is dissolved in toluene, adds ammonium acetate (6.40kg, 20eq), nitrogen is true
Empty substitution three times, is heated to 80-100 DEG C (outer temperature) and stirred 20 hours.TLC shows complete reaction, is cooled to room temperature (20 DEG C~25
℃).N-butanol, organic phase washed with water are added, saturated common salt water washing is concentrated to give 3.63kg compounds 5, yield 98.0%,
HPLC purity 99.04%.
The preparation (P Boc) of the compound 4 of embodiment 11
Starting compound 5 (2.13kg, 2.55mol, 1.0eq) is added in dichloromethane, 10-20 DEG C of addition, activity two
Manganese oxide (2.22kg, 10eq), nitrogen protection, 15 DEG C~20 DEG C stirring 10-15 hours, HPLC monitoring compound 5 are reacted completely,
Reaction solution is added into diatomite stirring, filtering, eluent methylene chloride, is concentrated to dryness, adds methyl tertiary butyl ether(MTBE), mass concentration is
(described mass concentration refers to that the quality of potassium hydroxide accounts for the hundred of potassium hydroxide aqueous solution gross mass to 10% potassium hydroxide aqueous solution
Divide ratio), overnight, filtering, filtrate is concentrated into small size, and (solvent volume of removing and the ratio of the molten cumulative volume of filtrate are for 60 DEG C of backflows
0.7) crystal seed, is added, stirs 2-3 hours, normal heptane is then added dropwise, 0-20 DEG C of stirring 1-2 hour, filtering, obtains 2.05kgization
Compound 4, yield 99.29%, HPLC purity 99.15%.
The preparation (P Boc) of the compound 4 of embodiment 12
Starting compound 5 (3.63kg, 4.38mol, 1.0eq) is added in dichloromethane, 10-20 DEG C of addition, activity two
Manganese oxide (3.81kg, 10eq), nitrogen protection, 5 DEG C~10 DEG C stirring 10-15 hours, HPLC monitoring compound 5 are reacted completely,
Reaction solution is added into diatomite stirring, filtering, eluent methylene chloride, is concentrated to dryness, adds methyl tertiary butyl ether(MTBE), mass concentration is
(described mass concentration refers to that the quality of potassium hydroxide accounts for the hundred of potassium hydroxide aqueous solution gross mass to 10% potassium hydroxide aqueous solution
Divide ratio), overnight, filtering, filtrate is concentrated into small size, and (solvent volume of removing and the ratio of the molten cumulative volume of filtrate are for 60 DEG C of backflows
0.7) crystal seed, is added, stirs 2-3 hours, normal heptane is then added dropwise, 0-20 DEG C of stirring 1-2 hour, filtering, obtains 3.62kgization
Compound 4, yield 90.0%, HPLC purity 99.30%.
The preparation of activated manganese dioxide：Weigh 20g manganese sulfates to be dissolved in 500mL distilled water, add 10mL concentrated ammonia liquors, shake
It is even, add 90mL ammonium persulfate solutions, stir while adding, boil 10min, then add 1~2 drop ammoniacal liquor, stand to clarification.Use Bu Shi
Funnel filters, and is washed 10 times with ammoniacal liquor, hot water wash 2~3 times, then is washed 12 times with sulfuric acid, is finally washed till no sulfuric acid reaction with hot water.In
110 DEG C of ± 5 DEG C of 3~4h of drying, are preserved in drier, standby.
The preparation (P Boc) of the compound 3 of embodiment 13
Compound 4 (2.05kg, 2.45mol, 1.0eq) is dissolved in methanol, addition trifluoromethanesulfonic acid (3.67kg,
10eq), (60 DEG C~65 DEG C) 2-6 hours are heated to reflux, TLC shows that compound 4 is reacted completely, is down to room temperature, is slowly dropped into and adds
Methyl tertiary butyl ether(MTBE), rear 0-10 DEG C of stirring 2-5 hour, filtering being added dropwise, solid is suspended in dichloromethane, adds ammoniacal liquor,
PH=11-12 is adjusted, 1h is stirred, filtering, organic phase washed with water, saturated sodium-chloride washing, concentration, obtains 1.66kg compounds 3,
Yield 93.5%, HPLC purity 99.30%.
The Wei Patawei 1 of embodiment 14 preparation
Addition MOC-L- valines (1.01kg 2.5eq) in reactor, I-hydroxybenzotriazole (HOBt) (0.84kg,
2.6eq), 1- ethyls-(3- dimethylaminopropyls) phosphinylidyne diimmonium salt hydrochlorate (EDCI) (1.1kg, 2.5eq) and acetonitrile 10-
15 DEG C are stirred 1 hour.0-10 DEG C is cooled to, compound 3 (1.66kg, 2.29mol, 1eq) is added, N, N- diisopropyls is then added dropwise
Base ethamine (DIPEA) (1.18kg, 3.5eq).0-15 DEG C, stir 12-16 hours.TLC detection compounds 3 are reacted completely.Slowly
Isopropyl acetate and water, filtering are added, liquid separation, organic phase uses 0.5N sodium hydroxides, saturated sodium-chloride water solution and washing successively
Wash, concentrate dry solvent, obtain 1.98kg Wei Patawei 1, yield 98.0%, HPLC purity 99.80%, maximum list miscellaneous 0.05%.
Claims (10)
1. a kind of preparation method of compound 12, it is characterised in that comprise the following steps：In organic solvent, bar existing for condensing agent
Under part, compound 13 and compound 9 are subjected to condensation reaction, compound 12 is made；
2. the preparation method of compound 12 as claimed in claim 1, it is characterised in that：
In the preparation method of described compound 12, described organic solvent is amide solvent；
And/or
In the preparation method of described compound 12, described condensing agent is 1- ethyls-(3- dimethylaminopropyls) phosphinylidyne
Diimmonium salt hydrochlorate, 2- (7- azos BTA)-N, N, N', N'- tetramethylurea hexafluorophosphoric acids ester, BTA-N,
N, N', N'- tetramethylurea hexafluorophosphoric acid ester, O- BTAs-N, N, N', N'- tetramethylurea tetrafluoro boric acid ester, benzo three
Azoles -1- three (dimethylamino)-trifluoro phosphate, chloro tripyrrole Wan Ji Phosphonium hexafluorophosphate, hexafluorophosphoric acid BTA -1-
Base-epoxide tripyrrole alkyl, N, N'- dicyclohexylcarbodiimides, (2- oximidos-cyan-acetic ester)-N, N- Dimethyl-morpholins
One or more in base urea hexafluorophosphoric acid ester, 2- oximes ethyl cyanoacetate and propylphosphonic anhydride；
And/or
In the preparation method of described compound 12, the molar ratio of described condensing agent and described compound 13 for 1~
3；
And/or
In the preparation method of described compound 12, the molar ratio of described compound 9 and described compound 13 for 1~
3；
And/or
In the preparation method of described compound 12, the temperature of described condensation reaction is 0 DEG C~40 DEG C；
And/or
In the preparation method of described compound 12, the time of described condensation reaction is 1 hour~10 hours；
And/or
The preparation method of described compound 12 uses following steps：0-10 DEG C, compound 13 and organic solvent are formed mixed
Compound, it is added drop-wise in the mixture that compound 9, condensing agent and organic solvent are formed, carries out condensation reaction and obtain described chemical combination
Thing 12.
3. the preparation method of compound 12 as claimed in claim 2, it is characterised in that：
In the preparation method of described compound 12, described amide solvent is DMF；
And/or
In the preparation method of described compound 12, the molar ratio of described condensing agent and described compound 13 for 1~
1.5；
And/or
In the preparation method of described compound 12, the molar ratio of described compound 9 and described compound 13 for 1~
1.5；
And/or
In the preparation method of described compound 12, the temperature of described condensation reaction is 10 DEG C~30 DEG C；
And/or
In the preparation method of described compound 12, the time of described condensation reaction is 2 hours~8 hours.
4. the preparation method of compound 12 as claimed in claim 1, it is characterised in that：
The preparation method of described compound 12 further comprises the preparation method of compound 13, and it comprises the following steps：It is organic
In solvent, compound 14 is carried out to the reaction of deaminizating protection group with acid, described compound 13 is made；
Wherein, P is amino protecting group.
5. the preparation method of compound 12 as claimed in claim 4, it is characterised in that：
In the preparation method of described compound 13, P is tertbutyloxycarbonyl, benzyloxycarbonyl group or p-toluenesulfonyl；
And/or
In the preparation method of described compound 13, described organic solvent is esters solvent；
And/or
In the preparation method of described compound 13, described acid is inorganic acid；
And/or
In the preparation method of described compound 13, the described sour and molar ratio of described compound 14 is 1~10；
And/or
In the preparation method of described compound 13, the temperature of the reaction of described removing amino protecting group is 40 DEG C~100
℃；
And/or
In the preparation method of described compound 13, the time of the reaction of described removing amino protecting group is 1 hour~10
Hour.
6. the preparation method of compound 12 as claimed in claim 5, it is characterised in that：
In the preparation method of described compound 13, described esters solvent is isopropyl acetate；
And/or
In the preparation method of described compound 13, described inorganic acid is hydrogen chloride；
And/or
In the preparation method of described compound 13, the described sour and molar ratio of described compound 14 is 4~6；
And/or
In the preparation method of described compound 13, the temperature of the reaction of described removing amino protecting group is 50 DEG C~70
℃；
And/or
In the preparation method of described compound 13, the time of the reaction of described removing amino protecting group is small for 2 hours~8
When.
7. the preparation method of compound 12 as claimed in claim 4, it is characterised in that：
The preparation method of described compound 12 further comprises the preparation method of compound 14, and it comprises the following steps：Protection
Under gas shield, in organic solvent, under the conditions of alkali is existing, compound 25 and compound 24 are subjected to condensation reaction, obtain institute
The compound 14 stated；
Wherein, P definition is as described in claim 4 or 5.
8. the preparation method of compound 12 as claimed in claim 7, it is characterised in that：
In the preparation method of described compound 14, described protective gas is nitrogen and/or argon gas；
And/or
In the preparation method of described compound 14, described organic solvent is amide solvent；
And/or
In the preparation method of described compound 14, described alkali is inorganic base；
And/or
In the preparation method of described compound 14, the molar ratio of described alkali and described compound 25 is 1~3；
And/or
In the preparation method of described compound 14, the molar ratio of described compound 24 and described compound 25 is 1
~3；
And/or
In the preparation method of described compound 14, the temperature of described condensation reaction is 10 DEG C~50 DEG C；
And/or
In the preparation method of described compound 14, the time of described condensation reaction is 1 hour~5 hours.
9. compound 12 and compound 13, its structure are as follows：
10. a kind of preparation method of compound 13, it is characterised in that it comprises the following steps：In organic solvent, by compound 14
The reaction of deaminizating protection group is carried out with acid, described compound 13 is made；
Wherein, P definition is as described in claim 4 or 5；Each reaction condition is as described in any one of claim 3~8.
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| CN113072615B (en) * | 2021-03-24 | 2023-01-10 | 上海法默生物科技有限公司 | Preparation method of vipatavir intermediate |
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| CN107573355A (en) | 2018-01-12 |
| CN107674062A (en) | 2018-02-09 |
| CN110698489B (en) | 2022-02-22 |
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| CN107573380A (en) | 2018-01-12 |
| CN107674064A (en) | 2018-02-09 |
| CN107674064B (en) | 2020-03-27 |
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| CN107759577B (en) | 2020-03-27 |
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