CN107759274A - A kind of production method of organic alga fertilizer - Google Patents
A kind of production method of organic alga fertilizer Download PDFInfo
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- CN107759274A CN107759274A CN201710914017.1A CN201710914017A CN107759274A CN 107759274 A CN107759274 A CN 107759274A CN 201710914017 A CN201710914017 A CN 201710914017A CN 107759274 A CN107759274 A CN 107759274A
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- algae
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- slag
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
Abstract
The present invention provides a kind of production method of organic alga fertilizer, and this method mainly includes：Algae discarded object, which crushes, to be mixed, enzymolysis decomposes alga cells wall, polysaccharide and protein, separation of solid and liquid enzymolysis liquid, medicinal extract is made with alcohol steep enzymolysis algae-residue again, enzymolysis liquid and medicinal extract are mixed to form organic liquid seaweed extract, organic marine alga granular fertilizer can be formed after mixing a certain proportion of algae-residue, the compound fertilizer of organic marine alga is can be made into after appropriate chemical fertilizer is added.The present invention prepares seaweed organic fertilizer using waste caused by tangleweed processing and microalgae process as raw material, and not only easy to operate, fabrication cycle is short, improves the environment friendly of enterprise's production and processing, improves Business Economic Benefit.
Description
Technical field
The invention belongs to agriculture and forestry organic waste material recycling and organic fertilizer production field, it is related to a kind of production of organic alga fertilizer
Method, and in particular to enzymatic isolation method degrades in tangleweed and microalgae process caused discarded object to produce organic alga fertilizer
Method and in this approach manufactured organic alga fertilizer and organic marine alga composite fertilizer.
Background technology
Chinese large-sized marine algae resource enriches, and annual production accounts for Gross World Product more than half up to more than 700 ten thousand tons.By rich
Rich tangleweed resource, China have been built up tangleweed production in large scale and secondary industry, formed with brown alga and
Red algae is the tangleweed industrial system of main body, is related to the multiple fields such as food, feed, medicine, chemical industry, turns into marine economy
Important component.Chinese large-sized marine alga processing at present relates generally to the algin such as extraction such as alginate, agar-agar, carragheen and added
Work.The substantial amounts of processing for being difficult to degrade can be produced in algin production process, after phycocolloid is extracted from tangleweed to give up
Gurry.By taking sea-tangle as an example, in sodium alginate extraction process, the sea-tangle after immersion digests by alkali lye, and coarse filtration obtains insoluble
The suspended solid discarded object that property component and glue obtain after diluting, foam, float is kelp residue.Calculate in proportion, often
Year China kelp processing industry at least produces number with 100,000 tons of kelp processing waste.And processing waste contains and largely had
Machine matter and nutritive salt, by taking kelp processing waste as an example, kelp residue is the solid impurity that ratio is maximum in kelp processing waste.
In terms of dry, mainly contain crude protein about 20%, crude fibre about 50%, ash content about 3% in kelp residue.In addition, in kelp residue also
Containing not extracting complete alginate, alginic acid sugar sulfuric acid ester, phytokinin and growth hormone isoreactivity material.If will
Tangleweed processing waste is directly invested in environment, this can turn into that body eutrophication and red tide induce it is potential because
Element, have a strong impact on the development of sea fishery economy and ecology.And tangleweed processing waste is carried out using the method for landfill
Processing, not only occupies substantial amounts of soil, also wastes substantial amounts of resource, therefore carries out marine alga slag for comprehensive research on utilization to lifting
The competitiveness of China's tradition sea-tangle industry, reduction environmental pollution all have great importance.To tangleweed processing waste
Second development and utilization, the efficiency of tangleweed processing technology can be effectively improved, sea is produced using tangleweed processing waste
Algae fertilizer, it is an effective way to turn waste into wealth.
The content of the invention
It is caused present invention aim to address tangleweed in the prior art and microalgae processing waste utilization rate be not high
Waste discharge pollutes in environment and the problem of the wasting of resources, there is provided it is a kind of efficiently, simply from algae processing waste
Extract the method for the organic alga fertilizer of nutriment acquisition and organic alga fertilizer and organic marine alga are compound made of such a method
Fertilizer.
The purpose of the present invention is realized by the following method：
A kind of production method of organic alga fertilizer, comprises the following steps：
(1) tangleweed is washed, smashing is dried in vacuum press dehydration, and the seawood meal of uniform particle diameter is made in sieving；
(2) algae-residue is washed, freeze-drying is standby；
(3) A. mixes the marine alga ground-slag treated by step 1) and the microalgae slag of step 2) processing, marine alga slag and microalgae
Slag mass ratio is 1-2: 1, is suspended with pH 4.5-6.0 acetic acid-sodium acetate buffer solution, by enzyme：Algae-residue gross mass is 2-2.5%
Cellulose degraded alga cells wall is added, 45-65 DEG C of temperature, extracts 36-72h, high temperature enzyme deactivation work 4-8min；B. adjust pH to
3.5-5.0 by enzyme：Algae-residue gross mass is that 1-2% adds pectase degradation of cell wall, algin and polysaccharide, 40-50 DEG C of temperature,
48-72h is extracted, enzyme deactivation is lived；C. pH to 5.5-6.5 is adjusted, by enzyme：Algae-residue gross mass is that 1-2.5% adds protease degraded
Protein, 45-65 DEG C of temperature extract 24-48h, and enzyme deactivation is lived.
(4) above-mentioned algae-residue enzymolysis liquid is subjected to standing separation of solid and liquid, obtains digesting supernatant；
(5) it is 1.0-1.2g/mL above-mentioned enzymolysis supernatant to be concentrated in vacuo into proportion；
(6) by the algae-residue alcohol steep 12-24h after step 3) processing, extraction supernatant is through the obtained proportion that is concentrated under reduced pressure
1.1-1.2g/mL medicinal extract, with obtained enzymolysis liquid in step 5), matched by medicinal extract and enzymolysis liquid mass volume ratio 5-10% mixed
Close, the organic alga fertilizer of liquid is made.
Further, the marine alga ground-slag and step 2) that the step 3) treats for A. mixings by step 1) handle micro-
Algae-residue, marine alga slag are 1-3: 1 with microalgae slag mass ratio, are suspended with pH 5.0 acetic acid-sodium acetate buffer solution, by enzyme：Algae-residue matter
Amount 55 DEG C of temperature, extracts 72h, 100 DEG C of enzyme deactivations work 5min than adding 2% cellulose degrading enzyme；B. pH 4.2 is adjusted, by enzyme：Algae
Slag mass ratio adds 1% pectase, temperature 50 C, extracts 72h, 100 DEG C of enzyme deactivations work 5min；C. pH 6.0 is adjusted, by enzyme：Algae-residue
Mass ratio adds 1% plasmase, 55 DEG C of temperature, extracts 48h, 100 DEG C of enzyme deactivations work 5min.
Beneficial effect：
(1) present invention, by reasonably optimizing reaction conditions, maximumlly decomposes algae by the way of combination digests
The organic substance in discarded object is produced, is easily to be absorbed by plants by macromolecules degradations such as cellulose therein, protein and polysaccharide
Small organic molecule, easy to operate, fabrication cycle is short；And using the remaining plant growth regulator of alcohol steep extraction, will
Enzymolysis liquid mixes with ethanol extract, is finally made organic alga fertilizer rich in nutriment and plant growth regulator.
(2) present invention prepares seaweed organic fertilizer using waste caused by tangleweed processing and microalgae process as raw material,
It is not only the new approaches that tangleweed or microalgae processing enterprise provide processing discarded object, improves the environment-friendly of enterprise's production and processing
Property, the comprehensive utilization of marine alga and microalgae process of manufacture is promoted, improves Business Economic Benefit.
Embodiment
The invention will now be further described with reference to specific embodiments, but embodiment is only exemplary, not to this hair
Bright scope forms any restrictions.It will be understood by those skilled in the art that without departing from the spirit and scope of the invention
The details and form of technical solution of the present invention can be modified or replaced, but these modifications and replacement each fall within the present invention's
In protection domain.
Embodiment 1
A kind of production method of organic alga fertilizer, comprises the following steps：
(1) tangleweed is washed, smashing is dried in vacuum press dehydration, and the seawood meal of uniform particle diameter is made in sieving；
(2) algae-residue is washed, freeze-drying is standby；
(3) A. mixes the marine alga ground-slag treated by step 1) and the microalgae slag of step 2) processing, marine alga slag and microalgae
Slag mass ratio is 1: 1, is suspended with pH 4.5 acetic acid-sodium acetate buffer solution, by enzyme：Algae-residue gross mass is 2% addition cellulose
Enzyme degraded alga cells wall, temperature 45 C, extract 36h, high temperature enzyme deactivation work 4min；B. pH to 3.5 is adjusted, by enzyme：The total matter of algae-residue
Measure and add pectase degradation of cell wall, algin and polysaccharide for 1%, 40 DEG C of temperature extracts 48h, and enzyme deactivation is lived；C. adjust pH to
5.5, by enzyme：Algae-residue gross mass is 1% addition protease protein degradation matter, and temperature 45 C extracts 24h, and enzyme deactivation is lived.
(4) above-mentioned algae-residue enzymolysis liquid is subjected to standing separation of solid and liquid, obtains digesting supernatant；
(5) it is 1.0g/mL above-mentioned enzymolysis supernatant to be concentrated in vacuo into proportion；
(6) by the algae-residue alcohol steep 12h after step 3) processing, extraction supernatant is through the obtained proportion 1.1- that is concentrated under reduced pressure
1.2g/mL medicinal extract, with obtained enzymolysis liquid in step 5), mixed by medicinal extract with enzymolysis liquid mass volume ratio 5-10% proportionings,
The organic alga fertilizer of liquid is made.
Embodiment 2
A kind of production method of organic alga fertilizer, comprises the following steps：
(1) tangleweed is washed, smashing is dried in vacuum press dehydration, and the seawood meal of uniform particle diameter is made in sieving；
(2) algae-residue is washed, freeze-drying is standby；
(3) A. mixes the marine alga ground-slag treated by step 1) and the microalgae slag of step 2) processing, marine alga slag and microalgae
Slag mass ratio is 2: 1, is suspended with pH 6.0 acetic acid-sodium acetate buffer solution, by enzyme：Algae-residue gross mass is 2.5% addition fiber
Plain enzyme degraded alga cells wall, 65 DEG C of temperature, extract 72h, high temperature enzyme deactivation work 8min；B. pH to 5.0 is adjusted, by enzyme：Algae-residue is total
Quality is 2% addition pectase degradation of cell wall, algin and polysaccharide, 40-50 DEG C of temperature, extracts 48-72h, and enzyme deactivation is lived；C. adjust
PH to 6.5 is saved, by enzyme：Algae-residue gross mass is 2.5% addition protease protein degradation matter, 45-65 DEG C of temperature, extracts 24-
48h, enzyme deactivation are lived.
(4) above-mentioned algae-residue enzymolysis liquid is subjected to standing separation of solid and liquid, obtains digesting supernatant；
(5) it is 1.2g/mL above-mentioned enzymolysis supernatant to be concentrated in vacuo into proportion；
(6) by the algae-residue alcohol steep 24h after step 3) processing, extraction supernatant is through the obtained proportion that is concentrated under reduced pressure
1.2g/mL medicinal extract, with obtained enzymolysis liquid in step 5), mixed by medicinal extract with enzymolysis liquid mass volume ratio 5-10% proportionings,
The organic alga fertilizer of liquid is made.
Embodiment 3
A kind of production method of organic alga fertilizer, comprises the following steps：
(1) tangleweed is washed, smashing is dried in vacuum press dehydration, and the seawood meal of uniform particle diameter is made in sieving；
(2) algae-residue is washed, freeze-drying is standby；
(3) A. mixes the marine alga ground-slag treated by step 1) and the microalgae slag of step 2) processing, marine alga slag and microalgae
Slag mass ratio is 1.5: 1, is suspended with pH 5.0 acetic acid-sodium acetate buffer solution, by enzyme：Algae-residue gross mass adds for 2-2.5%
Cellulose degraded alga cells wall, 55 DEG C of temperature, extract 55h, high temperature enzyme deactivation work 5min；B. pH to 4.0 is adjusted, by enzyme：Algae
Slag gross mass is that 1-2% adds pectase degradation of cell wall, algin and polysaccharide, and temperature 45 C extracts 60h, and enzyme deactivation is lived；C. adjust
PH to 6.0 is saved, by enzyme：Algae-residue gross mass is that 1-2.5% adds protease protein degradation matter, 45-65 DEG C of temperature, extracts 24-
48h, enzyme deactivation are lived.
(4) above-mentioned algae-residue enzymolysis liquid is subjected to standing separation of solid and liquid, obtains digesting supernatant；
(5) it is 1.1g/mL above-mentioned enzymolysis supernatant to be concentrated in vacuo into proportion；
(6) by the algae-residue alcohol steep 15h after step 3) processing, extraction supernatant is through the obtained proportion 1.1- that is concentrated under reduced pressure
1.2g/mL medicinal extract, with obtained enzymolysis liquid in step 5), mixed by medicinal extract with enzymolysis liquid mass volume ratio 5-10% proportionings,
The organic alga fertilizer of liquid is made.
Claims (2)
1. a kind of production method of organic alga fertilizer, it is characterised in that comprise the following steps：
(1) tangleweed is washed, smashing is dried in vacuum press dehydration, and the seawood meal of uniform particle diameter is made in sieving；
(2) algae-residue is washed, freeze-drying is standby；
(3) A. mixes the marine alga ground-slag treated by step 1) and the microalgae slag of step 2) processing, marine alga slag and microalgae slag
Amount is suspended, by enzyme than being 1-2: 1 with pH 4.5-6.0 acetic acid-sodium acetate buffer solution：Algae-residue gross mass adds for 2-2.5%
Cellulose degraded alga cells wall, 45-65 DEG C of temperature, extract 36-72h, high temperature enzyme deactivation work 4-8min；B. pH to 3.5- is adjusted
5.0, by enzyme：Algae-residue gross mass is that 1-2% adds pectase degradation of cell wall, algin and polysaccharide, 40-50 DEG C of temperature, extraction
48-72h, enzyme deactivation are lived；C. pH to 5.5-6.5 is adjusted, by enzyme：Algae-residue gross mass is that 1-2.5% adds protease protein degradation
Matter, 45-65 DEG C of temperature extract 24-48h, and enzyme deactivation is lived；
(4) above-mentioned algae-residue enzymolysis liquid is subjected to standing separation of solid and liquid, obtains digesting supernatant；
(5) it is 1.0-1.2g/mL above-mentioned enzymolysis supernatant to be concentrated in vacuo into proportion；
(6) by the algae-residue alcohol steep 12-24h after step 3) processing, extraction supernatant is through the obtained proportion 1.1- that is concentrated under reduced pressure
1.2g/mL medicinal extract, with obtained enzymolysis liquid in step 5), mixed by medicinal extract with enzymolysis liquid mass volume ratio 5-10% proportionings,
The organic alga fertilizer of liquid is made.
A kind of 2. production method of organic alga fertilizer described in claim 1, it is characterised in that：Step 3) is that A. is mixed by step
The rapid marine alga ground-slag 1) treated and the microalgae slag of step 2) processing, marine alga slag is 1-3: 1 with microalgae slag mass ratio, with pH 5.0
Acetic acid-sodium acetate buffer solution suspend, by enzyme：Algae-residue mass ratio adds 2% cellulose degrading enzyme, 55 DEG C of temperature, extracts 72h,
100 DEG C of enzyme deactivations work 5min；B. pH 4.2 is adjusted, by enzyme：Algae-residue mass ratio adds 1% pectase, temperature 50 C, extracts 72h,
100 DEG C of enzyme deactivations work 5min；C. pH 6.0 is adjusted, by enzyme：Algae-residue mass ratio adds 1% plasmase, 55 DEG C of temperature, extraction
48h, 100 DEG C of enzyme deactivations work 5min.
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| CN201710914017.1A CN107759274A (en) | 2017-09-30 | 2017-09-30 | A kind of production method of organic alga fertilizer |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108770882A (en) * | 2018-06-06 | 2018-11-09 | 广西宾德利生物科技有限公司 | Earthworm microalgae small-molecular peptides and the preparation method and application thereof |
| CN111543252A (en) * | 2020-05-08 | 2020-08-18 | 福建环海生物科技股份有限公司 | Seaweed gel extraction residue post-treatment process, fungus culture medium and fungus culture method |
| CN112479782A (en) * | 2021-01-15 | 2021-03-12 | 晟丰(烟台)农业科技有限公司 | Seaweed fertilizer and preparation method thereof |
| CN112592943A (en) * | 2021-01-15 | 2021-04-02 | 晟丰(烟台)农业科技有限公司 | Extraction method of seaweed liquid |
| CN112661543A (en) * | 2021-01-26 | 2021-04-16 | 晟丰(烟台)农业科技有限公司 | Seaweed fertilizer and preparation method thereof |
| CN113046246A (en) * | 2019-12-26 | 2021-06-29 | 云南爱尔发生物技术股份有限公司 | Culture solution preparation method based on microalgae culture |
| CN115487099A (en) * | 2022-10-28 | 2022-12-20 | 珠海市雅莎医疗器械有限公司 | Lifting and tightening skin barrier repair dressing and preparation method and application thereof |
-
2017
- 2017-09-30 CN CN201710914017.1A patent/CN107759274A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108770882A (en) * | 2018-06-06 | 2018-11-09 | 广西宾德利生物科技有限公司 | Earthworm microalgae small-molecular peptides and the preparation method and application thereof |
| CN113046246A (en) * | 2019-12-26 | 2021-06-29 | 云南爱尔发生物技术股份有限公司 | Culture solution preparation method based on microalgae culture |
| CN111543252A (en) * | 2020-05-08 | 2020-08-18 | 福建环海生物科技股份有限公司 | Seaweed gel extraction residue post-treatment process, fungus culture medium and fungus culture method |
| CN112479782A (en) * | 2021-01-15 | 2021-03-12 | 晟丰(烟台)农业科技有限公司 | Seaweed fertilizer and preparation method thereof |
| CN112592943A (en) * | 2021-01-15 | 2021-04-02 | 晟丰(烟台)农业科技有限公司 | Extraction method of seaweed liquid |
| CN112661543A (en) * | 2021-01-26 | 2021-04-16 | 晟丰(烟台)农业科技有限公司 | Seaweed fertilizer and preparation method thereof |
| CN115487099A (en) * | 2022-10-28 | 2022-12-20 | 珠海市雅莎医疗器械有限公司 | Lifting and tightening skin barrier repair dressing and preparation method and application thereof |
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