CN107753143A - Build the method and system of gastric lymphoma animal model - Google Patents
Build the method and system of gastric lymphoma animal model Download PDFInfo
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- CN107753143A CN107753143A CN201610679832.XA CN201610679832A CN107753143A CN 107753143 A CN107753143 A CN 107753143A CN 201610679832 A CN201610679832 A CN 201610679832A CN 107753143 A CN107753143 A CN 107753143A
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Abstract
The present invention proposes the method and system of structure gastric lymphoma animal model, and this method includes：Doubtful stomach organization sample is seeded in animal body；The animal is cultivated, to obtain the animal with knurl body；And the animal with knurl body is screened, to obtain the animal to form gastric lymphoma.Gastric lymphoma animal model can be obtained using this method.
Description
Technical field
The present invention relates to biomedicine field field, in particular it relates to build the side of gastric lymphoma animal model
Method and system.
Background technology
Tumour is body under the effect of various tumorigenesis factors, and the cell of local organization loses on gene level to be grown to it
Normal regulation cause paraplasm with differentiation and formed neoformation.Neoformation does not stop once being formed because the cause of disease eliminates
Growth, his growth destroy normal structure and organ not by normal body Physiological effect, this point in malignant tumour especially
Substantially.Compared with benign tumour, malignant growth speed is fast, and in infiltrative growth, bleeding, necrosis, ulcer etc. easily occurs, and
Often there is DISTANT METASTASES IN, cause human body to become thin, be powerless, anaemia, poor appetite, heating and serious organ function are impaired etc., most
Death is caused eventually.
Gastric lymphoma, which is meant, is primary in stomach and the malignant tumour originating from submucosa lymphoid tissue, or whole body is pernicious
A part for lymthoma.Secondary gastric lymphoma then may occur in which the constitutional symptoms such as heating, weight loss, liver, splenomegaly.
At present, gastric lymphoma is needed further to be furtherd investigate.
The content of the invention
It is contemplated that at least solves one of technical problem in correlation technique to a certain extent.
The present invention is that the present inventor is completed based on following discovery：
Inventor has found, if the sample of doubtful stomach organization is inoculated into immunodeficient mouse, in Mice Body
The doubtful patients with gastric cancer tumor model of itself is rebuild, is sought peace the response feature to medicine in the biological characteristics for remaining tumor tissues
On the basis of, individual of the screening with gastric lymphoma knurl body, the mouse for forming gastric lymphoma is obtained, multi-medicament and joint can be used
Prescription case is tested, and finds out accurate individual therapeutic regimen, to obtain best gastric lymphoma clinical efficacy, and then can have
The individuation treatment of cancer of effect ground guiding gastric lymphoma tumor patient, reduce the blindness of clinical application and reduce clinical application
Error rate.
Therefore, in the first aspect of the present invention, the present invention proposes a kind of method for building gastric lymphoma animal model.Root
According to embodiments of the invention, methods described includes：Doubtful stomach organization sample is seeded in animal body；The animal is carried out
Culture, to obtain the animal with knurl body；And the animal with knurl body is screened, to form stomach leaching to obtain
The animal of bar knurl.According to an embodiment of the invention, inventor has found, by using the side of above-mentioned structure gastric lymphoma animal model
Method, obtaining animal model effectively can test multi-medicament and scheme of combination drug therapy, find out accurately individual and use
Prescription case can effectively guide the individuation cancer of gastric lymphoma tumor patient to obtain best gastric lymphoma clinical efficacy
Treatment, reduce the blindness of clinical application and reduce the error rate of clinical application.
According to an embodiment of the invention, the method for above-mentioned structure gastric lymphoma animal model can further include as follows
At least one additional technical feature：
According to an embodiment of the invention, the animal is mouse.Inventor has found that inoculation animal uses mouse, Ke Yiyou
Effect shortens the time of model construction, improves the success rate of model construction.
According to an embodiment of the invention, the animal is immunodeficient mouse.Inventor find, immunodeficient mouse for
The rejection of heterograft is relatively low, and then further increases the success rate of model construction.
According to an embodiment of the invention, the animal is that NCG mouse are the mouse for having hyperimmunization defect, background list
First, heterograft survival rate height, long lifespan, inventor are had found, gastric lymphoma animal model, success rate are built from NCG mouse
Can further it improve, advantageously in the progress of later stage drug evaluation experiment.
According to an embodiment of the invention, described doubtful stomach organization sample is seeded in animal body is in the following way
Realize：Doubtful stomach organization sample is seeded under Animal Skin.Inventor is found through experiments that, subcutaneous vaccination, advantageously
Further improved in the survival and growth being vaccinated in animal body, the success rate of model construction in seed sample.
According to an embodiment of the invention, the volume of the doubtful stomach organization sample is 2*2*2mm3.Inventor passes through reality
Issue after examination and approval now, the volume of doubtful stomach organization sample is 2*2*2mm3, be advantageous to stomach organization sample and be vaccinated in animal body soon
Fast-growing is grown, and the success rate and speed of model construction can all significantly improve.
According to an embodiment of the invention, the time of the culture is 30~40 days.Inventor has found that incubation time is more than 40
My god, tumor volume is excessive, tumor ischemia necrosis, and incubation time is shorter than 30 days, and tumor volume is too small, and the pathology identification of knurl body is not
It is high, it is difficult to which that the classification of knurl body is judged.
According to an embodiment of the invention, the volume of the knurl body is 100~200mm3.Inventor has found that tumor volume exists
100~200mm3, the judgement to knurl body classification is more accurately and reliably.
According to an embodiment of the invention, the screening is carried out by haematine-eosin stains and SABC.Soviet Union
Another name for-eosin stains and SABC, the class of knurl body from the angle of the form of knurl body cell, biochemical angle and gene, can be judged
Type, the degree of accuracy are high, so obtain formed gastric lymphoma animal success rate it is high, be subsequently used for screening medicine, sieve medicine result can
Reliability further improves.
According to an embodiment of the invention, the screening is further detected by STR is carried out.STR detections can be from the angle of gene
Degree judges whether knurl body derives from same individual with doubtful stomach organization sample, and then further increases the success of model construction
Rate, it is subsequently used for screening medicine, the confidence level of sieve medicine result further improves.
In the second aspect of the present invention, the present invention proposes a kind of system for building gastric lymphoma animal model.According to this
The embodiment of invention, the system include：Classification inoculation apparatus, the classification inoculation apparatus are dynamic for doubtful stomach organization sample to be seeded to
In object；Culture apparatus, the culture apparatus is used to cultivate the animal, to obtain the animal with knurl body；With
And screening plant, the screening plant are used to screen the animal with knurl body, and gastric lymphoma is formed to obtain
Animal.It is surprisingly found by the inventors that can build gastric lymphoma animal model using the system, the animal model of structure can
Effectively to test multi-medicament and scheme of combination drug therapy, accurate individual therapeutic regimen is found out to obtain best stomach
Lymthoma clinical efficacy, the individuation treatment of cancer of gastric lymphoma tumor patient can be effectively guided, reduces clinical application
Blindness and reduce clinical application error rate.
According to an embodiment of the invention, the method for the system of above-mentioned structure gastric lymphoma animal model can also be wrapped further
Include at least one following additional technical feature：
According to an embodiment of the invention, the animal is mouse, and optionally, the mouse is immunodeficient mouse, optionally
Ground, the mouse are NCG mouse, and optionally, described doubtful stomach organization sample is seeded in animal body is by such as lower section
What formula was realized：Doubtful stomach organization sample is seeded under Animal Skin, optionally, the volume of the doubtful stomach organization sample is
2*2*2mm3, optionally, the time of culture is 30~40 days, and optionally, the volume of the knurl body is 100~200mm3.Above-mentioned
Optionally under additional technical feature, using the system of structure gastric lymphoma animal model according to embodiments of the present invention, stomach is built
The speed and success rate of lymphoma animal models significantly improve, and the gastric lymphoma animal model obtained is used to screen medicine, sieves
The success rate of medicine and the confidence level of sieve medicine result can all significantly improve.
According to an embodiment of the invention, the screening plant further comprises：Haematine-eosin stains unit, immune group
Change unit.Above-mentioned detection unit can be analyzed form, biochemistry and the gene of the cell of knurl body tissue samples, and then can be more
Add the type for judging knurl body exactly, and then more accurately screen the animal to form gastric lymphoma, the gastric lymphoma of acquisition
Animal for Subsequent pharmacological screen, sieve medicine result confidence level also further improve.
According to an embodiment of the invention, the screening plant further comprises STR detection units, and STR detection units can be from
The angle of gene judges knurl body and doubtful gastric lymphoma samples sources in same individual, and then the structure of the animal model of gastric lymphoma
Build up power further to improve, the confidence level of gastric lymphoma animal model further improves.
Brief description of the drawings
Fig. 1 is the system diagram of structure gastric lymphoma animal model according to embodiments of the present invention；
Fig. 2 is the structure chart of screening plant according to embodiments of the present invention；
Fig. 3 is the structure chart of screening plant according to embodiments of the present invention；
Fig. 4 is the Hematoxylin-eosin coloration result figure that determination knurl body according to embodiments of the present invention is gastric lymphoma；
Fig. 5 is the ImmunohistochemistryResults Results figure that determination knurl body according to embodiments of the present invention is gastric lymphoma；
Fig. 6 is that determination knurl body according to embodiments of the present invention and the STR detections of doubtful gastric lymphoma tissue identical source are tied
Fruit is schemed；And
Fig. 7 is the result figure according to embodiments of the present invention that medicine is screened using gastric lymphoma animal model.
Embodiment
Embodiments of the invention are described below in detail.The embodiments described below is exemplary, is only used for explaining this hair
It is bright, and be not considered as limiting the invention.
The method for building gastric lymphoma animal model
In one aspect of the invention, the present invention proposes a kind of method for building gastric lymphoma animal model.According to this
The embodiment of invention, this method include：Doubtful stomach organization sample is seeded in animal body；The animal is cultivated,
To obtain the animal with knurl body；And the animal with knurl body is screened, to form gastric lymphoma to obtain
Animal.According to an embodiment of the invention, inventor has found, by using the method for above-mentioned structure gastric lymphoma animal model,
Obtained animal model can be tested effectively multi-medicament and scheme of combination drug therapy, find out accurate individual prescription
Case can effectively guide the individuation cancer of gastric lymphoma tumor patient to control to obtain best gastric lymphoma clinical efficacy
Treat, reduce the blindness of clinical application and reduce the error rate of clinical application.
It should be noted that " doubtful stomach organization sample " of the present invention can be derived from vitro tissue, convenient material drawing,
Do not limited by surgical operation materials.
It should be noted that " inoculation " of the present invention operates, it is not particularly limited, those skilled in the art can be light
Pine is completed, and is not required to professional training.
According to a particular embodiment of the invention, inventor passes through patient is postoperative or the fresh doubtful gastric cancer tumor of biopsy
Tissue is inoculated into immunodeficient mouse body, after into knurl, to being screened into knurl animal, and the formation gastric lymphoma animal of acquisition
Medicament screening experiment can be carried out, finds out accurate individual therapeutic regimen to obtain best gastric lymphoma clinical efficacy.It will suffer from
The doubtful gastric cancer tumor tissue cut off in person's operation is inoculated into immunodeficient mouse, and patient itself has been rebuild in Mice Body
Tumor model, can best retain the response feature that the biological characteristics of tumor tissues are sought peace to medicine, therefore can be more accurate
Curative effect of medication is predicted on ground, and can avoid invalid chemotherapy and possible toxic side effect.The gastric lymphoma that the technology obtains
Animal can be tested multi-medicament and scheme of combination drug therapy, find out accurate individual therapeutic regimen to obtain best clinic
Curative effect, the individualized treatment of gastric lymphoma tumor patient can be effectively guided, reduce the blindness of clinical application.
According to a particular embodiment of the invention, the animal is mouse, preferably immunodeficient mouse, more preferably NCG mouse.
Inventor has found that the growth cycle of mouse is short, can greatly improve the growth rate of knurl body, can be maximum from immunodeficient mouse
The exclusion heterograft rejection of limit, increase the success rate of tumour transplatation, from NCG mouse, the genetic background list of mouse
First, heterograft has higher success rate, and the life-span of NCG mouse is longer, and it is real to be advantageous to the longer drug screening of subsequent cycle
The progress tested.
In addition, according to a particular embodiment of the invention, it is by connecing that doubtful stomach organization sample, which is seeded in animal body,
Realized under planting to Animal Skin.In the course of the study, inventor is had found, doubtful stomach organization sample is seeded into Animal Skin
Under, the survival rate of knurl body is higher, and the growth rate of knurl body is faster.Meanwhile inventor is in an experiment, it was also found that doubtful stomach organization
Sample size be preferably 2*2*2mm3Cube, under this size and shape, the survival rate after tumor inoculation is higher, growth
Speed is also higher.
According to another specific example of the present invention, after inoculation, animal is cultivated 30~40 days, and tumor size reaches 100~
200mm3It is the Best Times for the animal that screening forms knurl body.Inventor has found that animal incubation time is too short, and tumor volume exists
100mm3Hereinafter, the identification of knurl body type is not high, and pathology and groupization result are still similar to stomach cancer, and incubation time is long, knurl body
Volume is more than 200mm3, knurl known from experience because volume is excessive, causes knurl body tissue ischemia dead.The animal-free medium time is 30~40
My god, tumorous size is 100~200mm3, the animal for forming gastric lymphoma, the lymthoma of acquisition are screened on this time and size
Animal model is more true and reliable, and for screening the animal for the treatment of gastric lymphoma, sieve medicine result is also more true and reliable and has
Specific aim.
Further, according to the specific example of invention, the screening is by haematine-eosin stains, SABC and can
What the STR detections of choosing were carried out.Hematoxylin-eosin coloration result can provide the cellular morphology information of knurl body tissue, and SABC can
The Biochemical Information of knurl body tissue is provided, STR detections can judge the source of knurl body sample and the gene of sample from the angle of gene
Expressing information, and then judge simultaneously from cellular morphology, Biochemical Information and gene expression information, to sentencing for the species of knurl body sample
It is fixed more accurately gastric lymphoma animal model to be avoided to build by mistake, and cause the failure of even more serious sieve medicine experiment.
The system for building gastric lymphoma animal model
In another aspect of this invention, the present invention proposes a kind of system for building gastric lymphoma animal model.Reference picture 1
The system includes：
Classification inoculation apparatus 100, the classification inoculation apparatus 100 are used to doubtful stomach organization sample being seeded in animal body.According to
The specific embodiment of the present invention, the animal is mouse, preferably immunodeficient mouse, more preferably NCG mouse.According to the present invention's
Another specific embodiment, the position of the inoculation is under Animal Skin.According to the still another embodiment of the present invention, doubtful stomach is selected
The volume of cancerous tissue sample is 2*2*2mm3Cube., can be accurate by doubtful stomach organization sample under above-mentioned classification inoculation apparatus 100
Really successfully it is inoculated into animal body, success ratio of inoculation is high.
Culture apparatus 200, the culture apparatus 200 is used to cultivate the animal, to obtain with knurl body
Animal.According to a particular embodiment of the invention, the incubation time is 30~40 days, or the volume of knurl body is 100~200mm3
In the case of, the pathology identification of knurl body is high, and the activity of knurl body is high, is screened on this condition, obtains gastric lymphoma and moves
The success rate of thing model is high.
Screening plant 300, the screening plant 300 is used to screen the animal with knurl body, to obtain
Form the animal of gastric lymphoma.According to the specific example of the present invention, with reference to figure 2, the screening plant 300 further comprises：Soviet Union
Another name for-eosin stains unit 310, SABC unit 320.According to a particular embodiment of the invention, haematine-eosin stains
The testing result to knurl body tissue of unit 310 is " tumour cell diffuses distribution, and cell size is consistent, circular, oval, core
It is circular, oval, visible with a large amount of necrosis, nuclear fission " be gastric lymphoma instruction, SABC unit 320 is to knurl body tissue
Testing result be " CKpan (-), CEA (-), CD20 (3+), CD79 α (3+), CD43 (3+), CD3 (-), Ki-67 about 60%
(+) " is the instruction of gastric lymphoma.According to the another specific embodiment of the present invention, with reference to figure 3, the screening plant 300 is further
Including STR detections (short tandem repeat, the detection of short-movie section repetitive sequence parting) unit 330, wherein, STR detections are single
The testing result of first 330 pairs of knurl body tissues is " it is to be examined mouse tumor body sample with doubtful gastric lymphoma tissue to have that LR, which is more than 1000 ",
There is the instruction of identical source.Based on the testing result of above three detection unit, section result is sentenced accurately and reliably to knurl body classification,
Built gastric lymphoma animal model is true and reliable, is further used for the medicine or medication combined of screening treatment gastric lymphoma, sieves medicine
As a result it is also more true and reliable.
It should be noted that " method of structure gastric lymphoma animal model " described herein and " structure gastric lymphoma
The system of animal model " is used to treat gastric lymphoma medicine or medicine to obtain gastric lymphoma animal model as direct purpose
Thing screens in combination.Simultaneously as clinical stomach cancer is similar with the CT diagnostic results of stomach lymph cancer at present, method described herein
It also can use with system and be indirectly for stomach cancer and stomach lymph cancer being distinguished, the diagnosis for clinical stomach lymph cancer provides foundation.
In addition, it is necessary to explanation, enters in the gastric lymphoma animal model that method or system using the present invention are obtained
During row antineoplaston option screening, the screening of used antineoplaston scheme includes the screening of anti-gastric lymphoma medicine,
The screening of dosage, the management during the screening of screening, the radiotherapy of chemotherapy regimen, administration etc..Wherein, those skilled in the art
Can using conventional use of therapy in any this area or the medicine researched and developed come as candidate therapy, in addition, this
Art personnel can also use the existing medicine of various dose to carry out different dosing cycle and agent quantifier elimination.On anti-swollen
Tumor medicine, both can be Western medicine or Chinese medicine, and its type, which is not particularly restricted, (as appropriate for chemotherapy, molecular targeted to be controlled
The medicine for the treatment of, immunization therapy etc.).The therapeutic scheme formulated according to clinic, it can be determined according to the method for administration of different pharmaceutical real
The administering mode (including oral, hypodermic injection, intravenous injection etc.) of animal is tested, administration frequency enters according to patient clinical application method
OK.
Below by specific embodiment, the present invention will be described, if be not specifically stated, in embodiment below
Employed in material and method be known or can be obtained by buying.
Embodiment 1
In the present embodiment, the method that inventor describes structure gastric lymphoma animal model in detail.
1) experiment material
Tumor tissues：Clinic provides fresh doubtful gastric cancer tumor tissue, and tumor tissues are stored in tissue preserration liquid, 4 DEG C
Transport.
2) experimental animal
Kind：NCG mouse
Age：5-6 week old
Supplier：Model animal research institute of Nanjing University
Feeding environment：Model animal research institute of Nanjing University SPF experimental animal rooms
Laboratory temperature:22℃-25℃
Relative humidity：40%-70%
Relative atmospheric pressure：10Pa-20Pa
Air exchange frequency：(10-15) secondary/hour
Light cycle：Illumination (8 in 12 hours:00-20:00), 12 hours dark (20:00-8:00)
Animal feeding：5 animals/cages
Central air-conditioning and air filtration machinery equipment, animal cage, drinking-water, bedding and padding etc. are had through disinfection with high pressure steam,
Raise with the grains dedicated feed of SPF levels, free water.
3) reagent
Tissue preserration liquid, tissue culture medium, antibiotic etc..
4) experiment content
A, draw materials
Doubtful gastric lymphoma patient, by way of biopsy or operation, obtain tumor tissues.
Tissue preserration liquid, 4 DEG C of transports are placed in after materials at once, 12H is sent in hour.
Inoculation
Inoculation work is completed in the tumor tissues 24H of materials.Biopsy or surgical tissue are cut into about 2*2*2mm3
Size, the standard inoculation by 1 piece/are subcutaneous in mouse.Mice Inoculated quantity 5-10 only, by tumor tissues amount determined by particular number
It is fixed.
Biopsy samples are less, are completely used for being inoculated with.
B, observation and measurement
Before terminating after inoculation to experiment, animal state is observed daily, if animals got sick or abnormal growth are found, by beast
Doctor is handled or dissected.
The measurement of tumour growth situation：With vernier caliper measurement gross tumor volume size and carry out tumor-bearing mice once in a week
Weighing body weight.
Gross tumor volume calculation formula：V(mm3)=(a × b2)/2, a:Tumour major diameter, b:Tumour minor axis
C, experiment and materials are terminated
If P1 has tumour to grow for animal (first generation animal after inoculation), choose the most fast tumor volume of growth and reach
To 100-200mm3,Peel off knurl body tissue and fix preservation, for doing Hematoxylin-eosin dyeing.If three months after inoculation, in P1 generations, are swollen
Knurl then puts to death this batch of mouse, terminates the model still without growth sign.
D, sample detection
Fixed knurl body tissue P1 and P0 (tumor tissues sample) are done into Hematoxylin-eosin dyeing and immunohistochemical analysis
And STR detections.
E, interpretation of result
With reference to primary tumor tissues P0 Hematoxylin-eosin coloration result, comparative analysis P1/P0 Hematoxylin-eosin
Dyeing, SABC and STR examining reports, determine the type of knurl body.
Wherein it is determined that knurl body is the Hematoxylin-eosin coloration result of gastric lymphoma as shown in figure 4, Fig. 4 left hand views are P0 groups
Finding under mirror is knitted, right part of flg is finding under the mirror of P1 knurl body tissues, and P1 knurl body tissues show as tumour cell and diffuse distribution, cell
In the same size, circular, oval, core is circular, oval, and kernel unobvious, nuclear fission is more common, and sees a large amount of thick liquid cell leachings
Profit.
Wherein it is determined that knurl body for gastric lymphoma ImmunohistochemistryResults Results as shown in figure 5, P1 knurl body tissues SABC inspection
Survey result show as tumour cell CKpan (-), CEA (-), CD20 (3+), CD79 α (3+), CD43 (3+), CD3 (being dispersed in+),
Ki-67 about 70% (+).
Wherein it is determined that P1 and P0 comes from the STR testing results of same individual as shown in fig. 6, the STR of P1 knurl body tissues is detected
As a result LR (likelihood ratio, likelihood ratio) 5.7825 × 10 is shown as27, more than 1000.
Knurl body is as characterized above, shows that gastric lymphoma animal model successfully constructs.
Embodiment 2
In the present embodiment, inventor using the gastric lymphoma animal model successfully constructed treat the medicine of gastric lymphoma
Thing screens, and experimentation and result are as described below.
P1, into knurl animal, treats that knurl body reaches 800-1000mm for other3, knurl body is peeled off, knurl body is trimmed to 2*2*2mm3Greatly
It is small, by 1 piece/of standard inoculation, 50 mouse are inoculated with, when knurl body reaches 150mm3Random packet carries out pharmacodynamics test afterwards,
Optimal treatment gastric lymphoma scheme is filtered out to refer to for clinical treatment.In the present embodiment, medicine to be screened includes：A Pa is replaced
Buddhist nun, taxol, oxaliplatin, Irinotecan, 5-Fu, Ah pa for Buddhist nun+taxol, Ah pa for Buddhist nun+oxaliplatin, Ah pa for Buddhist nun+
Irinotecan, Ah pa replace Buddhist nun+5-Fu, and the selection result is as shown in fig. 7, Fig. 7 results are shown, in the medicine screened, Ah's pa replaces
Buddhist nun+Irinotecan is optimal to the inhibition of gastric lymphoma.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or the spy for combining the embodiment or example description
Point is contained at least one embodiment or example of the present invention.In this manual, to the schematic representation of above-mentioned term not
Identical embodiment or example must be directed to.Moreover, specific features, structure, material or the feature of description can be with office
Combined in an appropriate manner in one or more embodiments or example.In addition, in the case of not conflicting, the skill of this area
Art personnel can be tied the different embodiments or example and the feature of different embodiments or example described in this specification
Close and combine.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, changed, replacing and modification.
Claims (10)
- A kind of 1. method for building gastric lymphoma animal model, it is characterised in thatDoubtful stomach organization sample is seeded in animal body；The animal is cultivated, to obtain the animal with knurl body；AndThe animal with knurl body is screened, to obtain the animal to form gastric lymphoma.
- 2. according to the method for claim 1, it is characterised in that the animal is mouse,Optionally, the mouse is immunodeficient mouse,Optionally, the mouse is NCG mouse.
- 3. according to the method for claim 1, it is characterised in that described that doubtful stomach organization sample is seeded in animal body Realize in the following way：Doubtful stomach organization sample is seeded under Animal Skin.
- 4. according to the method for claim 1, it is characterised in that the volume of the doubtful stomach organization sample is 2*2*2mm3。
- 5. according to the method for claim 1, it is characterised in that the time of the culture is 30~40 days.
- 6. according to the method for claim 1, it is characterised in that the volume of the knurl body is 100~200mm3。
- 7. according to the method for claim 1, it is characterised in that the screening is by haematine-eosin stains and is immunized What groupization was carried out,Optionally, the screening is further detected by STR is carried out.
- A kind of 8. system for building gastric lymphoma animal model, it is characterised in that including：Classification inoculation apparatus, the classification inoculation apparatus are used to doubtful stomach organization sample being seeded in animal body；Culture apparatus, the culture apparatus is used to cultivate the animal, to obtain the animal with knurl body；Screening plant, the screening plant are used to screen the animal with knurl body, and stomach lymph is formed to obtain The animal of knurl.
- 9. system according to claim 8, it is characterised in that the animal is mouse,Optionally, the mouse is immunodeficient mouse；Optionally, the mouse is NCG mouse；Optionally, described doubtful stomach organization sample is seeded in animal body is realized in the following way：Doubtful stomach organization sample is seeded under Animal Skin,Optionally, the volume of the doubtful stomach organization sample is 2*2*2mm3,Optionally, the time of culture is 30~40 days,Optionally, the volume of the knurl body is 100~200mm3。
- 10. system according to claim 8, it is characterised in that the screening plant further comprises：Haematine-Yihong Dye unit and SABC unit,Optionally, the screening plant further comprises STR detection units.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108835051A (en) * | 2018-06-01 | 2018-11-20 | 郑州大学第附属医院 | Utilize immune deficiency nude mice model constructed by lymphoma cell strain |
| CN115399292A (en) * | 2022-04-01 | 2022-11-29 | 上海米地生物医药有限公司 | Construction and application of primary extralymph node lymphoma mouse model |
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- 2016-08-17 CN CN201610679832.XA patent/CN107753143A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108835051A (en) * | 2018-06-01 | 2018-11-20 | 郑州大学第附属医院 | Utilize immune deficiency nude mice model constructed by lymphoma cell strain |
| CN115399292A (en) * | 2022-04-01 | 2022-11-29 | 上海米地生物医药有限公司 | Construction and application of primary extralymph node lymphoma mouse model |
| CN115399292B (en) * | 2022-04-01 | 2024-04-02 | 上海米地生物医药有限公司 | Construction and application of primary extra-lymph node lymphoma mouse model |
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Application publication date: 20180306 |