CN107741421A - A kind of food safety detection reagents and preparation method thereof - Google Patents
A kind of food safety detection reagents and preparation method thereof Download PDFInfo
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- CN107741421A CN107741421A CN201711224091.7A CN201711224091A CN107741421A CN 107741421 A CN107741421 A CN 107741421A CN 201711224091 A CN201711224091 A CN 201711224091A CN 107741421 A CN107741421 A CN 107741421A
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- silver
- salt
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- detection
- food safety
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- 238000001514 detection method Methods 0.000 title claims abstract description 89
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 54
- 235000013305 food Nutrition 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 30
- -1 polyoxyethylene Polymers 0.000 claims abstract description 24
- 229910052751 metal Inorganic materials 0.000 claims abstract description 23
- 239000002184 metal Substances 0.000 claims abstract description 23
- 239000000203 mixture Substances 0.000 claims abstract description 21
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims abstract description 16
- SDKPSXWGRWWLKR-UHFFFAOYSA-M sodium;9,10-dioxoanthracene-1-sulfonate Chemical compound [Na+].O=C1C2=CC=CC=C2C(=O)C2=C1C=CC=C2S(=O)(=O)[O-] SDKPSXWGRWWLKR-UHFFFAOYSA-M 0.000 claims abstract description 16
- 235000017858 Laurus nobilis Nutrition 0.000 claims abstract description 15
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims abstract description 15
- 244000125380 Terminalia tomentosa Species 0.000 claims abstract description 15
- 235000005212 Terminalia tomentosa Nutrition 0.000 claims abstract description 15
- 239000012266 salt solution Substances 0.000 claims abstract description 15
- 239000001509 sodium citrate Substances 0.000 claims abstract description 15
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 15
- JAJWGJBVLPIOOH-IZYKLYLVSA-M sodium taurocholate Chemical compound [Na+].C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 JAJWGJBVLPIOOH-IZYKLYLVSA-M 0.000 claims abstract description 15
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical group [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000011591 potassium Substances 0.000 claims abstract description 14
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 14
- NDVLTYZPCACLMA-UHFFFAOYSA-N silver oxide Chemical compound [O-2].[Ag+].[Ag+] NDVLTYZPCACLMA-UHFFFAOYSA-N 0.000 claims abstract description 14
- GGCZERPQGJTIQP-UHFFFAOYSA-N sodium;9,10-dioxoanthracene-2-sulfonic acid Chemical compound [Na+].C1=CC=C2C(=O)C3=CC(S(=O)(=O)O)=CC=C3C(=O)C2=C1 GGCZERPQGJTIQP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 150000003057 platinum Chemical class 0.000 claims abstract description 10
- QXDMQSPYEZFLGF-UHFFFAOYSA-L calcium oxalate Chemical compound [Ca+2].[O-]C(=O)C([O-])=O QXDMQSPYEZFLGF-UHFFFAOYSA-L 0.000 claims abstract description 9
- ZXSQEZNORDWBGZ-UHFFFAOYSA-N 1,3-dihydropyrrolo[2,3-b]pyridin-2-one Chemical compound C1=CN=C2NC(=O)CC2=C1 ZXSQEZNORDWBGZ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 150000003839 salts Chemical class 0.000 claims abstract description 8
- 229910001958 silver carbonate Inorganic materials 0.000 claims abstract description 8
- LKZMBDSASOBTPN-UHFFFAOYSA-L silver carbonate Substances [Ag].[O-]C([O-])=O LKZMBDSASOBTPN-UHFFFAOYSA-L 0.000 claims abstract description 8
- 229910001961 silver nitrate Inorganic materials 0.000 claims abstract description 8
- JEOOVNWNJKBHRG-UHFFFAOYSA-N [Na+].[S--].[S--].[Au+3] Chemical compound [Na+].[S--].[S--].[Au+3] JEOOVNWNJKBHRG-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229910001923 silver oxide Inorganic materials 0.000 claims abstract description 7
- CLSUSRZJUQMOHH-UHFFFAOYSA-L platinum dichloride Chemical compound Cl[Pt]Cl CLSUSRZJUQMOHH-UHFFFAOYSA-L 0.000 claims abstract description 6
- CQLFBEKRDQMJLZ-UHFFFAOYSA-M silver acetate Chemical group [Ag+].CC([O-])=O CQLFBEKRDQMJLZ-UHFFFAOYSA-M 0.000 claims abstract description 6
- 229940071536 silver acetate Drugs 0.000 claims abstract description 6
- 229940071575 silver citrate Drugs 0.000 claims abstract description 6
- FBEIPJNQGITEBL-UHFFFAOYSA-J tetrachloroplatinum Chemical group Cl[Pt](Cl)(Cl)Cl FBEIPJNQGITEBL-UHFFFAOYSA-J 0.000 claims abstract description 6
- QUTYHQJYVDNJJA-UHFFFAOYSA-K trisilver;2-hydroxypropane-1,2,3-tricarboxylate Chemical compound [Ag+].[Ag+].[Ag+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QUTYHQJYVDNJJA-UHFFFAOYSA-K 0.000 claims abstract description 6
- 229960001516 silver nitrate Drugs 0.000 claims abstract description 4
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 claims description 28
- 239000007864 aqueous solution Substances 0.000 claims description 21
- 239000004971 Cross linker Substances 0.000 claims description 20
- HWXBTNAVRSUOJR-UHFFFAOYSA-N alpha-hydroxyglutaric acid Natural products OC(=O)C(O)CCC(O)=O HWXBTNAVRSUOJR-UHFFFAOYSA-N 0.000 claims description 14
- 229940009533 alpha-ketoglutaric acid Drugs 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000010521 absorption reaction Methods 0.000 claims description 7
- 230000000052 comparative effect Effects 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- 230000001473 noxious effect Effects 0.000 claims description 7
- 231100000167 toxic agent Toxicity 0.000 claims description 7
- 239000003440 toxic substance Substances 0.000 claims description 7
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 6
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims description 2
- 229910052697 platinum Inorganic materials 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 239000003431 cross linking reagent Substances 0.000 claims 1
- 125000003963 dichloro group Chemical group Cl* 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 5
- 230000035945 sensitivity Effects 0.000 abstract description 4
- KPGXRSRHYNQIFN-UHFFFAOYSA-L 2-oxoglutarate(2-) Chemical compound [O-]C(=O)CCC(=O)C([O-])=O KPGXRSRHYNQIFN-UHFFFAOYSA-L 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 description 18
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 6
- 244000025254 Cannabis sativa Species 0.000 description 6
- 239000011575 calcium Substances 0.000 description 6
- 229910052791 calcium Inorganic materials 0.000 description 6
- 229910052709 silver Inorganic materials 0.000 description 3
- 239000004332 silver Substances 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
- NMLQNVRHVSWEGS-UHFFFAOYSA-N [Cl].[K] Chemical group [Cl].[K] NMLQNVRHVSWEGS-UHFFFAOYSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- RRXWRHLYVPTSIF-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;silver Chemical compound [Ag].OC(=O)CC(O)(C(O)=O)CC(O)=O RRXWRHLYVPTSIF-UHFFFAOYSA-N 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- CTENFNNZBMHDDG-UHFFFAOYSA-N Dopamine hydrochloride Chemical compound Cl.NCCC1=CC=C(O)C(O)=C1 CTENFNNZBMHDDG-UHFFFAOYSA-N 0.000 description 1
- 208000019331 Foodborne disease Diseases 0.000 description 1
- 229920000877 Melamine resin Polymers 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- QSDSNNSKORVORL-UHFFFAOYSA-N acetic acid;silver Chemical group [Ag].CC(O)=O QSDSNNSKORVORL-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 239000003653 coastal water Substances 0.000 description 1
- 229960001149 dopamine hydrochloride Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- JDSHMPZPIAZGSV-UHFFFAOYSA-N melamine Chemical compound NC1=NC(N)=NC(N)=N1 JDSHMPZPIAZGSV-UHFFFAOYSA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/775—Indicator and selective membrane
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Spectroscopy & Molecular Physics (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses a kind of food safety detection reagents, the detection reagent includes the metal salt solution of sodium taurocholate, polyoxyethylene laurel ether, alpha Ketoglutarate, calcium oxalate, sodium citrate and reproducibility；The metal salt is one or more compositions in gold salt, silver salt, platinum salt；The gold salt is potassium chloroaurate, gold sodium sulfide and gold sodium chloride；The silver salt is silver acetate, silver nitrate, silver citrate, silver carbonate and silver oxide；The platinum salt is platinum tetrachloride, platinous chloride and potassium chloroplatinate.The detection reagent of the present invention can quickly, easy detection food, detection speed is fast, high sensitivity, and testing result is directly perceived, accurately；The detection reagent preparation method of the present invention is simple, and cost is relatively low, and is easy to carry, and is not required to specialized instrument and equipment and professional, easy promotion and implementation.
Description
Technical field
The present invention relates to technical field of food detection, specifically a kind of food safety detection reagents and preparation method thereof.
Background technology
Food-safety problem has been a global topic, and food security malignant event constantly occurs in the world, causes
Huge economic loss.Food-safety problem mainly includes the following aspects：Chemical hazard, biotoxin, microbes
The safety issue of harm, food adulteration and Genetic engineering food.According to the report of the World Health Organization and Center for Disease Control,
The food pollution event 40% in Europe occurs in individual family.And only U.S., just there are 76,000,000 human hairs to give birth to food-borne disease every year
Disease and 5000 people are dead.In China, microorganism pollution, residues of pesticides, the pollution at the water source such as rivers and lakes and coastal waters and illegal
It is the unsafe key factor of food to add hormone and medicine etc..
Though advantage that existing food making method has high sensitivity, detection limit low etc., because expensive equipment, time-consuming,
Testing cost height waits and is difficult to the present situation for meeting that food-safety problem takes place frequently.Therefore, it is fast that a kind of low cost, detection speed are developed
Food inspection new method is extremely necessary.
The content of the invention
It is an object of the invention to provide a kind of food safety detection reagents and preparation method thereof, to solve above-mentioned background skill
The problem of being proposed in art.
To achieve the above object, the present invention provides following technical scheme：
A kind of food safety detection reagents, the detection reagent include sodium taurocholate, polyoxyethylene laurel ether, α-ketoglutaric acid, grass
The metal salt solution of sour calcium, sodium citrate and reproducibility；The metal salt is one or more in gold salt, silver salt, platinum salt
Composition；The gold salt is potassium chloroaurate, gold sodium sulfide and gold sodium chloride；The silver salt is silver acetate, silver nitrate, citric acid
Silver, silver carbonate and silver oxide；The platinum salt is platinum tetrachloride, platinous chloride and potassium chloroplatinate.
As the further scheme of the present invention：The gold salt is potassium chloroaurate, gold sodium sulfide and gold sodium chloride according to weight
Measure percentage 1：1.5：The composition of 1 mixing.
As the further scheme of the present invention：The silver salt is silver acetate, silver nitrate, silver citrate, silver carbonate and oxidation
Silver is according to percentage by weight 2：1：The composition of 1.5 mixing.
As the further scheme of the present invention：The platinum salt is platinum tetrachloride, platinous chloride and potassium chloroplatinate according to weight
Percentage 1.3:1.5:The composition of 2 mixing.
A kind of preparation method of food safety detection reagents, comprises the following steps：By sodium taurocholate, polyoxyethylene laurel ether,
The metal salt solution mixing of α-ketoglutaric acid, calcium oxalate, sodium citrate and reproducibility, 1- is stirred under 200-500r/min
2h, it is subsequently placed in ultrasonic wave and shakes 20-40min, produces detection reagent.
A kind of detection method of food safety detection reagents, comprises the following steps：（1）Take the detection examination of two parts of same volumes
Agent, and take water that is identical with aqueous solution volume to be detected and being free of toxicant solution as a comparison；（2）First part of detection is tried
Agent mixes with comparative solution, then adds 2-6M highly basic and 6-8M Biological cross-linkers, forms the first mixed liquor；By second part of inspection
Test agent mixes with the aqueous solution to be detected, then adds 2-6M highly basic and 6-8M Biological cross-linkers, forms the second mixed liquor；It is described
The ultimate density of highly basic is 0.001-0.1M, the final concentration of 0.001-0.1M of the Biological cross-linker；（3）With the first mixed liquor
For reference, the color change for observing the second mixed liquor or the UV absorption change for detecting the second mixed liquor determine the aqueous solution to be detected
With the presence or absence of noxious material.
Compared with prior art, the beneficial effects of the invention are as follows：
The detection reagent of the present invention can quickly, easy detection food, detection speed is fast, high sensitivity, and testing result is directly perceived, standard
Really；The detection reagent preparation method of the present invention is simple, and cost is relatively low, and is easy to carry, and is not required to specialized instrument and equipment and professional people
Member, easy promotion and implementation.
Embodiment
The technical scheme of this patent is described in more detail with reference to embodiment.
Embodiment 1
A kind of food safety detection reagents, the detection reagent include sodium taurocholate, polyoxyethylene laurel ether, α-ketoglutaric acid, grass
The metal salt solution of sour calcium, sodium citrate and reproducibility；The metal salt is gold salt；The gold salt is potassium chloroaurate, sulfurous acid
Golden sodium and gold sodium chloride are according to percentage by weight 1：1.5：The composition of 1 mixing.
A kind of preparation method of food safety detection reagents, comprises the following steps：By sodium taurocholate, polyoxyethylene laurel ether,
The metal salt solution mixing of α-ketoglutaric acid, calcium oxalate, sodium citrate and reproducibility, is stirred 1h, so under 200r/min
After be placed in ultrasonic wave and shake 20min, produce detection reagent.
A kind of detection method of food safety detection reagents, comprises the following steps：（1）Take the detection examination of two parts of same volumes
Agent, and take water that is identical with aqueous solution volume to be detected and being free of toxicant solution as a comparison；（2）First part of detection is tried
Agent mixes with comparative solution, then adds 2M highly basic and 6M Biological cross-linkers, forms the first mixed liquor；Second part of detection is tried
Agent mixes with the aqueous solution to be detected, then adds 2M highly basic and 6M Biological cross-linkers, forms the second mixed liquor；The highly basic is most
Final concentration of 0.001-0.1M, the final concentration of 0.001-0.1M of the Biological cross-linker；（3）Using the first mixed liquor as reference,
The color change for observing the second mixed liquor or the UV absorption change for detecting the second mixed liquor determine whether the aqueous solution to be detected is deposited
In noxious material.
Embodiment 2
A kind of food safety detection reagents, the detection reagent include sodium taurocholate, polyoxyethylene laurel ether, α-ketoglutaric acid, grass
The metal salt solution of sour calcium, sodium citrate and reproducibility；The metal salt is gold salt, the composition of silver salt；The gold salt is chlorine
Potassium aurate, gold sodium sulfide and gold sodium chloride are according to percentage by weight 1：1.5：The composition of 1 mixing.The silver salt is acetic acid
Silver, silver nitrate, silver citrate, silver carbonate and silver oxide are according to percentage by weight 2：1：The composition of 1.5 mixing.
A kind of preparation method of food safety detection reagents, comprises the following steps：By sodium taurocholate, polyoxyethylene laurel ether,
The metal salt solution mixing of α-ketoglutaric acid, calcium oxalate, sodium citrate and reproducibility, is stirred 2h, so under 500r/min
After be placed in ultrasonic wave and shake 40min, produce detection reagent.
A kind of detection method of food safety detection reagents, comprises the following steps：（1）Take the detection examination of two parts of same volumes
Agent, and take water that is identical with aqueous solution volume to be detected and being free of toxicant solution as a comparison；（2）First part of detection is tried
Agent mixes with comparative solution, then adds 6M highly basic and 8M Biological cross-linkers, forms the first mixed liquor；Second part of detection is tried
Agent mixes with the aqueous solution to be detected, then adds 6M highly basic and 8M Biological cross-linkers, forms the second mixed liquor；The highly basic is most
Final concentration of 0.001-0.1M, the final concentration of 0.001-0.1M of the Biological cross-linker；（3）Using the first mixed liquor as reference,
The color change for observing the second mixed liquor or the UV absorption change for detecting the second mixed liquor determine whether the aqueous solution to be detected is deposited
In noxious material.
Embodiment 3
A kind of food safety detection reagents, the detection reagent include sodium taurocholate, polyoxyethylene laurel ether, α-ketoglutaric acid, grass
The metal salt solution of sour calcium, sodium citrate and reproducibility；The metal salt is gold salt, silver salt, the composition of platinum salt；The gold salt
It is potassium chloroaurate, gold sodium sulfide and gold sodium chloride according to percentage by weight 1：1.5：The composition of 1 mixing.The silver salt is vinegar
Sour silver, silver nitrate, silver citrate, silver carbonate and silver oxide are according to percentage by weight 2：1：The composition of 1.5 mixing.The platinum salt
It is platinum tetrachloride, platinous chloride and potassium chloroplatinate according to percentage by weight 1.3:1.5:The composition of 2 mixing.
A kind of preparation method of food safety detection reagents, comprises the following steps：By sodium taurocholate, polyoxyethylene laurel ether,
The metal salt solution mixing of α-ketoglutaric acid, calcium oxalate, sodium citrate and reproducibility, 1.5h is stirred under 300r/min,
It is subsequently placed in ultrasonic wave and shakes 30min, produces detection reagent.
A kind of detection method of food safety detection reagents, comprises the following steps：（1）Take the detection examination of two parts of same volumes
Agent, and take water that is identical with aqueous solution volume to be detected and being free of toxicant solution as a comparison；（2）First part of detection is tried
Agent mixes with comparative solution, then adds 4M highly basic and 7M Biological cross-linkers, forms the first mixed liquor；Second part of detection is tried
Agent mixes with the aqueous solution to be detected, then adds 4M highly basic and 7M Biological cross-linkers, forms the second mixed liquor；The highly basic is most
Final concentration of 0.001-0.1M, the final concentration of 0.001-0.1M of the Biological cross-linker；（3）Using the first mixed liquor as reference,
The color change for observing the second mixed liquor or the UV absorption change for detecting the second mixed liquor determine whether the aqueous solution to be detected is deposited
In noxious material.
Embodiment 4
A kind of food safety detection reagents, the detection reagent include sodium taurocholate, polyoxyethylene laurel ether, α-ketoglutaric acid, grass
The metal salt solution of sour calcium, sodium citrate and reproducibility；The metal salt is gold salt, the composition of platinum salt；The gold salt is chlorine
Potassium aurate, gold sodium sulfide and gold sodium chloride are according to percentage by weight 1：1.5：The composition of 1 mixing.The platinum salt is four chlorinations
Platinum, platinous chloride and potassium chloroplatinate are according to percentage by weight 1.3:1.5:The composition of 2 mixing.
A kind of preparation method of food safety detection reagents, comprises the following steps：By sodium taurocholate, polyoxyethylene laurel ether,
The metal salt solution mixing of α-ketoglutaric acid, calcium oxalate, sodium citrate and reproducibility, 1.8h is stirred under 400r/min,
It is subsequently placed in ultrasonic wave and shakes 25min, produces detection reagent.
A kind of detection method of food safety detection reagents, comprises the following steps：（1）Take the detection examination of two parts of same volumes
Agent, and take water that is identical with aqueous solution volume to be detected and being free of toxicant solution as a comparison；（2）First part of detection is tried
Agent mixes with comparative solution, then adds 3M highly basic and 7M Biological cross-linkers, forms the first mixed liquor；Second part of detection is tried
Agent mixes with the aqueous solution to be detected, then adds 3M highly basic and 7M Biological cross-linkers, forms the second mixed liquor；The highly basic is most
Final concentration of 0.001-0.1M, the final concentration of 0.001-0.1M of the Biological cross-linker；（3）Using the first mixed liquor as reference,
The color change for observing the second mixed liquor or the UV absorption change for detecting the second mixed liquor determine whether the aqueous solution to be detected is deposited
In noxious material.
Embodiment 5
A kind of food safety detection reagents, the detection reagent include sodium taurocholate, polyoxyethylene laurel ether, α-ketoglutaric acid, grass
The metal salt solution of sour calcium, sodium citrate and reproducibility；The metal salt is silver salt；The silver salt is silver acetate, silver nitrate, lemon
Lemon acid silver, silver carbonate and silver oxide are according to percentage by weight 2：1：The composition of 1.5 mixing.
A kind of preparation method of food safety detection reagents, comprises the following steps：By sodium taurocholate, polyoxyethylene laurel ether,
The metal salt solution mixing of α-ketoglutaric acid, calcium oxalate, sodium citrate and reproducibility, 1.2h is stirred under 460r/min,
It is subsequently placed in ultrasonic wave and shakes 35min, produces detection reagent.
A kind of detection method of food safety detection reagents, comprises the following steps：（1）Take the detection examination of two parts of same volumes
Agent, and take water that is identical with aqueous solution volume to be detected and being free of toxicant solution as a comparison；（2）First part of detection is tried
Agent mixes with comparative solution, then adds 5M highly basic and 8M Biological cross-linkers, forms the first mixed liquor；Second part of detection is tried
Agent mixes with the aqueous solution to be detected, then adds 5M highly basic and 8M Biological cross-linkers, forms the second mixed liquor；The highly basic is most
Final concentration of 0.001-0.1M, the final concentration of 0.001-0.1M of the Biological cross-linker；（3）Using the first mixed liquor as reference,
The color change for observing the second mixed liquor or the UV absorption change for detecting the second mixed liquor determine whether the aqueous solution to be detected is deposited
In noxious material.
The detection reagent of the present invention can be used for detection formalin, melamine, Dopamine hydrochloride, sulphur etc..
The detection reagent of the present invention can quickly, easy detection food, detection speed soon, high sensitivity, testing result is straight
See, be accurate；The present invention detection reagent preparation method it is simple, cost is relatively low, and is easy to carry, be not required to specialized instrument and equipment and specially
Industry personnel, easy promotion and implementation.
The better embodiment of this patent is explained in detail above, but this patent is not limited to above-mentioned embodiment party
Formula, can also be on the premise of this patent objective not be departed from one skilled in the relevant art's possessed knowledge
Make a variety of changes.
Claims (6)
- A kind of 1. food safety detection reagents, it is characterised in that the detection reagent include sodium taurocholate, polyoxyethylene laurel ether, α-ketoglutaric acid, calcium oxalate, the metal salt solution of sodium citrate and reproducibility；The metal salt is in gold salt, silver salt, platinum salt One or more compositions；The gold salt is potassium chloroaurate, gold sodium sulfide and gold sodium chloride；The silver salt be silver acetate, Silver nitrate, silver citrate, silver carbonate and silver oxide；The platinum salt is platinum tetrachloride, platinous chloride and potassium chloroplatinate.
- 2. food safety detection reagents according to claim 1, it is characterised in that the gold salt is potassium chloroaurate, sulfurous The golden sodium of acid and gold sodium chloride are according to percentage by weight 1：1.5：The composition of 1 mixing.
- 3. food safety detection reagents according to claim 1, it is characterised in that the silver salt be silver acetate, silver nitrate, Silver citrate, silver carbonate and silver oxide are according to percentage by weight 2：1：The composition of 1.5 mixing.
- 4. food safety detection reagents according to claim 1, it is characterised in that the platinum salt is platinum tetrachloride, dichloro Change platinum and potassium chloroplatinate according to percentage by weight 1.3:1.5:The composition of 2 mixing.
- A kind of 5. preparation method of food safety detection reagents as described in claim 1-2 is any, it is characterised in that including with Lower step：By the metal salt solution of sodium taurocholate, polyoxyethylene laurel ether, α-ketoglutaric acid, calcium oxalate, sodium citrate and reproducibility Mixing, is stirred 1-2h under 200-500r/min, is subsequently placed in ultrasonic wave and shakes 20-40min, produce detection reagent.
- A kind of 6. detection method of food safety detection reagents as described in claim 1-2 is any, it is characterised in that including with Lower step：（1）The detection reagent of two parts of same volumes is taken, and is taken identical with aqueous solution volume to be detected and without toxicant Water solution as a comparison；（2）First part of detection reagent is mixed with comparative solution, then adds 2-6M highly basic and 6-8M biologies Crosslinking agent, form the first mixed liquor；Second part of detection reagent is mixed with the aqueous solution to be detected, then adds 2-6M highly basic and 6- 8M Biological cross-linkers, form the second mixed liquor；The ultimate density of the highly basic is 0.001-0.1M, the end of the Biological cross-linker Concentration is 0.001-0.1M；（3）Using the first mixed liquor as reference, color change or the mixing of detection second of the second mixed liquor are observed The UV absorption change of liquid determines that the aqueous solution to be detected whether there is noxious material.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109724971A (en) * | 2019-02-01 | 2019-05-07 | 莱华尔科技有限公司 | A kind of preparation method and applications of the food sensing solution using gold nanoparticle |
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| CN103558336A (en) * | 2013-10-24 | 2014-02-05 | 中国科学院宁波材料技术与工程研究所 | Portable food safety inspection reagent as well as preparation method and application thereof |
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| CN101403691A (en) * | 2008-11-14 | 2009-04-08 | 苏州艾杰生物科技有限公司 | Melamine or tricyanic acid measuring method and its reagent kit or test paper strip |
| CN103558336A (en) * | 2013-10-24 | 2014-02-05 | 中国科学院宁波材料技术与工程研究所 | Portable food safety inspection reagent as well as preparation method and application thereof |
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| CN109724971A (en) * | 2019-02-01 | 2019-05-07 | 莱华尔科技有限公司 | A kind of preparation method and applications of the food sensing solution using gold nanoparticle |
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