CN107736348A - A kind of preparation prevented and treated fruit and adopt rear grey mold disease - Google Patents

A kind of preparation prevented and treated fruit and adopt rear grey mold disease Download PDF

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Publication number
CN107736348A
CN107736348A CN201711039600.9A CN201711039600A CN107736348A CN 107736348 A CN107736348 A CN 107736348A CN 201711039600 A CN201711039600 A CN 201711039600A CN 107736348 A CN107736348 A CN 107736348A
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fruit
methyl esters
acid methyl
aqueous solution
thujic
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CN201711039600.9A
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CN107736348B (en
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田世平
陈彤
秦国政
徐勇
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Institute of Botany of CAS
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Institute of Botany of CAS
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing carboxylic groups or thio analogues thereof, directly attached by the carbon atom to a cycloaliphatic ring; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of preparation prevented and treated fruit and adopt rear grey mold disease.The preparation Wei thujic acids methyl esters aqueous solution, its mass-volume concentration is 0~6000mg/L, but is not zero；The method that preventing and treating fruit provided by the invention adopts rear gray mold, comprises the following steps：The Jie Zhong thujic acid methyl esters aqueous solution, that is, realize the preventing and treating that rear gray mold is adopted to fruit in the fruit infected to botrytis cinerea；The mass-volume concentration of the Suo Shu thujic acid methyl esters aqueous solution is 0~6000mg/L, but is not zero；The inoculum concentration of the Suo Shu thujic acid methyl esters aqueous solution is 5~10 μ L.The present invention has carried out system research for postharvest diseases of fruit occurrence regularity, and finds the inhibitory action of thujic acid methyl esters on this basis, and fruit disease is occurred for it and disease development has efficient prevention effect.Preventing and treating fruit provided by the invention adopts the agent thujic acid methyl esters processed of rear grey mold disease, can prevent and treat the fruit that botrytis cinerea infects, and fruit disease is occurred and disease development has efficient prevention effect.

Description

A kind of preparation prevented and treated fruit and adopt rear grey mold disease
Technical field
The present invention relates to a kind of preparation prevented and treated fruit and adopt rear grey mold disease, belong to biological technical field.
Background technology
Fruit is the important component in human diet structure, because its sugar content is high, adopt rear cracky and by germ Infection, fruit rot is caused, therefore it is the fresh-keeping key issue of fruit storage that rear infection process is adopted in preventing and treating.Gray mold is by sac fungus The pathogen of Botrytis cinerea (Botrytis cinerea) (also known as botrytis cinerea) in subphylum Botrytis causes, and is a kind of common and is difficult to The fungal disease of preventing and treating；The pathogen of Botrytis cinerea can damage to the stem of crop, leaf, flower, fruit, and the harm to fruit is particularly tight Weight, influences fruit yield and quality, causes great economic loss.
Chemical prevention is to prevent and treat the method that gray mold is most widely used, and its fragmentation effect is notable, simple to operate, often at present Chemical agent is mainly aminopyrimidine, pyroles and acid amide fungicides.But the long-term use of chemical agent makes ash The resistance to the action of a drug of botrytis constantly strengthens, and causes prevention effect to substantially reduce；In addition, above-mentioned a few series bactericidal agents are all hypertoxic medicines Agent, easily largely remained in crop, cause the noxious material that is remained in fruits and vegetables exceeded, be eaten for a long time and pole is caused to human body Major injury；On the other hand, a large amount of chemicals are accumulated in soil, cause the soil texture to change, and are brought to ecological environment huge Big harmfulness pressure.
More than 400,000 kinds secondary metabolites, wherein most of chemical substances can be produced in plant growth and development process, Such as terpenes, alkaloid, flavonoids, steroid and phenols, desinsection or antibacterial activity are respectively provided with, therefore, utilization plant money It is fresh-keeping very wide with disease control prospect that source is used for postharvest fruit and vegetable.Thujic acid (thujic acid) its esterification Wu thujic acid methyl esters (methyl thujate) is the natural materials extracted from Western Red Cedar (Thuja plicata), can be used as natural component Raw material is used for Cosmetic Manufacture, purification of air and treating of wood.
The content of the invention
It is an object of the invention to provide a kind of preparation prevented and treated fruit and adopt rear grey mold disease, thujic acid methyl esters can prevent and treat grey mold The fruit that bacterium is infected, fruit disease is occurred and disease development has efficient prevention effect.
Application of the present invention Ti Gong thujic acids methyl esters first in gray mold after preventing and treating fruit is adopted.
, is seeded in the post-harvest fruits that botrytis cinerea infects in the form of the thujic acid methyl esters aqueous solution in concrete application.
The mass-volume concentration of the Suo Shu thujic acid methyl esters aqueous solution can be 0~6000mg/L, but be not zero.
The inoculum concentration of the Suo Shu thujic acid methyl esters aqueous solution can be 5~10 μ L.
Fruit suitable for preventing and treating can be apple, pears and/or peach.
Invention further provides a kind of method prevented and treated fruit and adopt rear gray mold, comprise the following steps：
The Jie Zhong thujic acid methyl esters aqueous solution, that is, realize the preventing and treating that rear gray mold is adopted to fruit in the fruit infected to botrytis cinerea.
The mass-volume concentration of the Suo Shu thujic acid methyl esters aqueous solution can be 0~6000mg/L, but be not zero；
The inoculum concentration of the Suo Shu thujic acid methyl esters aqueous solution can be 5~10 μ L.
The fruit can be apple, pears and/or peach.
The present invention is using the observation bacterium colony extension of potato dextrose agar flat board, the suppression of preliminary Ping Jia thujic acid methyl esters Bacterium effect；And inoculation is stabbed by fruit, the order inoculation in situ through botrytis cinerea spore suspension and thujic acid methyl esters carries out antibacterial Effect analysis.The present invention has carried out system research for postharvest diseases of fruit occurrence regularity, and finds thujic acid on this basis The inhibitory action of methyl esters, fruit disease is occurred for it and disease development has efficient prevention effect.
Preventing and treating fruit provided by the invention adopts the agent thujic acid methyl esters processed of rear grey mold disease, can prevent and treat the fruit that botrytis cinerea infects It is real, fruit disease is occurred and disease development has efficient prevention effect.
Brief description of the drawings
Fig. 1 is the change that different thujic acid methyl esters adds the pathogen of Botrytis cinerea colony diameter under concentration in the embodiment of the present invention 1.
Fig. 2 is that Apple stabs suppression effect of the inoculation Hou , thujic acids methyl esters to the pathogen of Botrytis cinerea in the embodiment of the present invention 2 Fruit.
Fig. 3 is that pear fruit stabs inhibition of the inoculation Hou , thujic acids methyl esters to the pathogen of Botrytis cinerea in the embodiment of the present invention 3.
Fig. 4 is that Peach fruits stab inhibition of the inoculation Hou , thujic acids methyl esters to the pathogen of Botrytis cinerea in the embodiment of the present invention 4.
Embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
Material used, reagent etc., unless otherwise specified, are commercially obtained in following embodiments.
The grey mold spore suspension used in following embodiment 2-4 is prepared by the following method：
After the pathogen of Botrytis cinerea is cultivated into 7 days on PDA plate, with sterile deionized water cleaning down bacterium colony, afterwards with two Spore is collected by filtration in layer sterile gauze, and is counted under an optical microscope using blood counting chamber, by spore suspension Concentration is adjusted to 1 × 105Individual spore/mL.
Embodiment 1,
1mm is cut on the PDA plate with the pathogen of Botrytis cinerea (Botrytis cinerea) with transfer needle2With mycelia Culture block, be seeded in a diameter of 90mm PDA plate, cultivated in 25 DEG C of incubators.Wherein PDA culture medium Zhong thujic acids The concentration of methyl esters is respectively 60,120 and 180mg/L.
Colony diameter was measured every 12 hours afterwards, is measured 4 days altogether, each concentration for the treatment of measures 6 plates, as a result such as Fig. 1 institutes Show, for Ke to find out that , thujic acids methyl esters has significant inhibitory action to the pathogen of Botrytis cinerea, wherein Pei supports the concentration of Ji Zhong thujic acid methyl esters It is optimal for 180mg/L inhibition.
Embodiment 2, apple stab inhibition of the inoculation Hou thujic acids methyl esters to the pathogen of Botrytis cinerea
Coloring, uniform healthy Apple are chosen, soaks 2min using 2wt% liquor natrii hypochloritises, clear water is washed Naturally dry after net.
By transfer needle on alcolhol burner flame calcination 1min, be pierced at fruit center equator position after natural cooling, hole depth Spend for 3mm, diameter 3mm.
5 μ L grey mold spore suspensions are added in hole stabbing, concentration is 1 × 105Individual spore/mL, naturally dry 1h.
The 10 μ L thujic acid methyl esters aqueous solution are added in hole stabbing, concentration is respectively 600,3000 and 6000mg/L, control group Add 10 μ L sterilized waters.
Add to distill the blotting paper moisturizing of water-soaked, sealed with freshness protection package, observed every 24 hours and measure record fruit Real incidence, count lesion diameter and photograph to record, as shown in Figure 2.
Embodiment 3, pears stab inhibition of the inoculation Hou thujic acids methyl esters to the pathogen of Botrytis cinerea
Coloring, uniform healthy pear fruit are chosen, soaks 2min using 2% liquor natrii hypochloritis, clear water cleans it Naturally dry afterwards.
By transfer needle on alcolhol burner flame calcination 1min, be pierced at fruit center equator position after natural cooling, hole depth Spend for 3mm, diameter 3mm.
5 μ L grey mold spore suspensions are added in hole stabbing, concentration is 1 × 105Individual spore/mL, naturally dry 1h.
The 10 μ L thujic acid methyl esters aqueous solution are added in hole stabbing, concentration is respectively 600,3000 and 6000mg/L, control group Add 10 μ L sterilized waters.
Add to distill the blotting paper moisturizing of water-soaked, sealed with freshness protection package, observed every 24 hours and measure record fruit Real incidence, count lesion diameter and photograph to record, as shown in Figure 3.
Embodiment 4, peach stab inhibition of the inoculation Hou thujic acids methyl esters to the pathogen of Botrytis cinerea
Coloring, uniform healthy Peach fruits are chosen, soak 2min using 2% liquor natrii hypochloritis, clear water cleans it Naturally dry afterwards.
By transfer needle on alcolhol burner flame calcination 1min, be pierced at fruit center equator position after natural cooling, hole depth Spend for 3mm, diameter 3mm.
5 μ L grey mold spore suspensions are added in hole stabbing, concentration is 1 × 105Individual spore/mL, naturally dry 1h.
The 10 μ L thujic acid methyl esters aqueous solution are added in hole stabbing, concentration is respectively 600,3000 and 6000mg/L, control group Add 10 μ L sterilized waters.
Add to distill the blotting paper moisturizing of water-soaked, sealed with freshness protection package, observed every 24 hours and measure record fruit Real incidence, count lesion diameter and photograph to record, as shown in Figure 4.
As Fig. 2-Fig. 4 Ke to find out that , thujic acids methyl esters can significantly inhibit fruit gray mold caused by the pathogen of Botrytis cinerea infects, Access concentration is bigger, and inhibition is better.
It is above-mentioned test result indicates that, thujic acid methyl esters, which adopts rear grey mold disease to fruit, has significant inhibitory action, to fruit Disease occurs and disease development has prevention effect.

Claims (10)

  1. A kind of 1. preparation , Wei thujic acid methyl esters aqueous solution prevented and treated fruit and adopt rear gray mold.
  2. 2. preparation according to claim 1, it is characterised in that：The mass-volume concentration of the Suo Shu thujic acid methyl esters aqueous solution is 0 ~6000mg/L, but be not zero.
  3. 3. application of the thujic acid methyl esters in gray mold after preventing and treating fruit is adopted.
  4. 4. application according to claim 3, it is characterised in that：The thujic acid methyl esters aqueous solution is seeded to the fruit that botrytis cinerea infects In reality.
  5. 5. application according to claim 4, it is characterised in that：The mass-volume concentration of the Suo Shu thujic acid methyl esters aqueous solution is 0 ~6000mg/L, but be not zero.
  6. 6. the application according to claim 4 or 5, it is characterised in that：The inoculum concentration of the Suo Shu thujic acid methyl esters aqueous solution is 5~10 μL。
  7. 7. according to the application any one of claim 3-6, it is characterised in that：The fruit is apple, pears and/or peach.
  8. 8. a kind of method prevented and treated fruit and adopt rear gray mold, comprises the following steps：
    The Jie Zhong thujic acid methyl esters aqueous solution, that is, realize the preventing and treating that rear gray mold is adopted to fruit in the fruit infected to botrytis cinerea.
  9. 9. according to the method for claim 8, it is characterised in that：The mass-volume concentration of the Suo Shu thujic acid methyl esters aqueous solution is 0 ~6000mg/L, but be not zero；
    The inoculum concentration of the Suo Shu thujic acid methyl esters aqueous solution is 5~10 μ L.
  10. 10. method according to claim 8 or claim 9, it is characterised in that：The fruit is apple, pears and/or peach.
CN201711039600.9A 2017-10-30 2017-10-30 A kind of prevention and treatment fruit adopts the preparation of rear grey mold disease Active CN107736348B (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06329504A (en) * 1993-05-20 1994-11-29 Norin Suisansyo Shinrin Sogo Kenkyusho Mite and tick preventive
JPH09220383A (en) * 1995-12-15 1997-08-26 Michiko Nakatsuka Antibacterial material
JP2006307392A (en) * 2005-04-28 2006-11-09 Hajime Torimoto Antimicrobial natural fiber and method for producing the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06329504A (en) * 1993-05-20 1994-11-29 Norin Suisansyo Shinrin Sogo Kenkyusho Mite and tick preventive
JPH09220383A (en) * 1995-12-15 1997-08-26 Michiko Nakatsuka Antibacterial material
JP2006307392A (en) * 2005-04-28 2006-11-09 Hajime Torimoto Antimicrobial natural fiber and method for producing the same

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