CN1077288A - Simplify the immunological method and the kit of fast detecting hepatitis - Google Patents
Simplify the immunological method and the kit of fast detecting hepatitis Download PDFInfo
- Publication number
- CN1077288A CN1077288A CN 93103933 CN93103933A CN1077288A CN 1077288 A CN1077288 A CN 1077288A CN 93103933 CN93103933 CN 93103933 CN 93103933 A CN93103933 A CN 93103933A CN 1077288 A CN1077288 A CN 1077288A
- Authority
- CN
- China
- Prior art keywords
- solid phase
- kit
- reagent
- solid
- phase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to method and diagnostic kit that solid-phase immunity detects, its method is that bag is invested surface of solid phase carriers by the immune binding reagents of usefulness, label, known benchmark or quality-control sample etc. as a comparison, through sealing, low temperature drying etc., form the reagent of solid phase.It is numerous and diverse that the present invention has overcome traditional kit composition, and packing, transportation, storage inconvenience make kit quality susceptible to, and operation steps is many in the use, variable factor is relatively also many, the shortcoming that operate miss is big.Elisa kit of the present invention comprises: 12 hole bar solid-phase reagents, sample diluting liquid, chromogenic reagent; Putting the agent of being excused from an examination comprises: solid-phase reagent, dilution.
Description
The present invention relates to solid-phase immunity detection method and product.
During solid-phase immunity detected, the method for widespread use had: enzyme joint inspection survey technology (ELISA), put inspection-free survey technology etc.With enzyme joint inspection survey technology is example: it is the ultramicro test method that the high susceptibility with the specificity of immune association reaction and biological enzyme combines, use very extensively in the detection of viral hepatitis, acquired immune deficiency syndrome (AIDS) etc., the commercial kit of using this method preparation goes on the market in a large number.At present, this technology has developed fairly perfectly.Commercially available kit has two kinds of well-established law and express methods, the former is a two-step reaction, the latter is a single step reaction, it is example that its basic testing process detects hepatitis B surface antibody HBsAg with sandwich method: bag by good anti--the HBs(hepatitis B surface antibody) the carrier aperture in add testing sample (patients serum) 100ul, make negative control pipe (not containing HBsAg) and positive control pipe (containing HBsAg) simultaneously, incubation is 1.5～2 hours in 37 ℃ water-bath, inhale dereaction liquid, wash aperture three times with distilled water, in each hole, add the anti-HBs(HRP of HRP-again and represent horseradish peroxidase) 100ul, after in aqueous solution, cultivating 1～1.5 hour, inhale dereaction liquid, the same wash aperture after, add developer in each hole and carry out chromogenic reaction, color deeply feels more that to contain HBsAg in the bright sample many more, and negative control and positive control are used for the test kit quality, simultaneously, negative control is also as judging whether sample contains the reference standard of HBsAg.
Express method is exactly that above-mentioned two-step reaction is become single step reaction, shortens the incubation time simultaneously, and all the other are all with last same.
Above-mentioned enzyme-linked method kit comprises following content:
(1) HRP-resists-HBs solution and label dilution;
(2) anti--HBs coating buffer and carrier orifice plate or bag are by the carrier orifice plate of good anti--HBs;
(3) negative control sera;
(4) negative control sera;
(5) chromogenic reagent: comprise the substrate H2O2 of developer and enzyme and stop buffer etc.;
(6) accelerator (a step express method uses).
Radioimmunological kit comprises following content:
(1)
125The anti-HBs solution of I-;
(2) anti--HBs wraps by the hole;
(3) negative control sera;
(4) positive control serum;
(5) dilution
Putting inspection-free survey process surveys identical with the enzyme joint inspection.
From use angle, mainly there are two problems in prior art: the one, and kit is formed numerous and diverse, thereby makes packing, transportation, storage inconvenience, kit quality susceptible to; Two are to use middle operation steps many, and variable factor is relatively also many, thereby the operate miss of bringing is also big, directly has influence on measurement result.
The object of the present invention is to provide a kind of method of the step when simplifying kit and reduce using kit and the kit of producing by said method, reach further control kit quality, reduce operate miss, thereby the raising accuracy of detection has also been saved time and labour simultaneously widely.
The objective of the invention is to realize: at first will wrap by the immune binding reagents of usefulness and be coated onto surface of solid phase carriers by following method; seal processing then; so that surface of solid phase carriers does not reveal the activated adsorption point, protection simultaneously has been attached to the immunologic active material coating of surface of solid phase carriers.
Again with label (enzyme mark thing or
125The I label) invests carrier surface; in the solid phase carrier of appointment, add simultaneously known benchmark as a comparison or quality-control sample (as: negative control, positive control, standard items, quality controlled serum etc.) respectively; behind the low-temperature vacuum drying; add protective agent again, forming layer protecting film because of the phase reagent surface by low-temperature vacuum drying.
With the solid-phase reagent vacuum of making as stated above be encapsulated in the sealing bag product.
Simply more than the original reagent box with its kit of preparing, its enzyme-linked method kit comprises:
(1) 12 hole bar solid-phase reagent;
(2) sample diluting liquid;
(3) chromogenic reagent
Radioimmunological kit comprises:
(1) solid-phase reagent;
(2) dilution
Use this method can assemble to put and exempt from and elisa kit, as: HBsAg, anti--HBs, HBeAg, anti--HBe, anti--HBc or the like measured.
The present invention compares with the immunological method and the kit technology of traditional fast detecting hepatitis, has significant advantage:
(1) simplifies the component of kit, improved kit quality and stability.Used reagent in the immune response is generally protein matter, and this class material is " fragile " all very, and its immunocompetence is lost easily, thereby loses immune binding ability.This immunocompetence is easier under aqueous solution state to be subjected to factors of affecting and inactivation, as temperature, bubble, various bacteriums etc.Enzyme labeling thing in traditional kit, bag is solution state with antibody or antigen and positive and negative tester etc., and stablizing of kit is totally unfavorable for keeping, and the present invention all has been placed in the carrier mentioned reagent, and be dry product, thereby can keep the stable of its quality better
(2) kit that the present invention assembled, its immunoreagent is dry product, and is in air-tight state, helps transportation and preservation.
(3) quality of kit is easier to be controlled.Because the incident that former cause user is done has become reagent producer to unify to go to do, thereby can accomplish that more kit uses gained result's a property attained in different experiments, help the quality control between each laboratory, comparability is better.
(4) reduce user's operation steps, thereby reduced artificial detection error, shortened detection time, improved efficient, saved the labour and the energy.
The concrete implementation step of the present invention is as follows, to survey HBsAg is example: use solid phase carrier, added bag by the anti--HBs of usefulness at Kong Zhongxian, wrap one deck anti--HBs after, seal processing, be about to confining liquid and add carrier surface, low-temperature vacuum drying, its temperature can be controlled in-10～-12 ℃ more and adds the anti-HBs(of HRP-again put adding when exempting from each hole
125The anti-HBs solution of I-); simultaneously in first hole, add negative control sera; add positive control serum in second hole, add protection liquid again and carry out low-temperature vacuum drying (temperature conditions is same as above), the solid-phase reagent hole bar vacuum that said method is made be encapsulated in the sealing bag product.With the solid-phase reagent box that said method is made, enzyme-linked method comprises: (1) 12 hole bar solid-phase reagent; (2) chromogenic reagent; (3) sample diluting liquid.Putting the method for exempting from comprises: (1) solid phase carrier reagent; (2) dilution.
Claims (2)
1, a kind of immunological method of simplifying fast detecting hepatitis is characterized in that:
(1) at first will wrap by the immune binding reagents of usefulness and be coated onto surface of solid phase carriers;
(2) immunologic active material coating and the surface of solid phase carriers to surface of solid phase carriers seals processing, then low-temperature vacuum drying;
(3) label is invested carrier surface, in carrier hole, pearl or the pipe of appointment, add simultaneously known as benchmark or quality-control sample;
(4) add protective agent, low-temperature vacuum drying makes the solid-phase reagent surface form layer protecting film, then it is sealed in the bag.
2, the kit of the method for claim 1 production, it is characterized in that: the enzyme-linked method diagnostic kit comprises: 12 hole bar solid phase carrier reagent; Sample diluting liquid; Chromogenic reagent; Put and exempt from diagnostic kit and comprise: solid phase carrier reagent, dilution; Above-mentioned solid phase carrier reagent comprises: the outer field bag of solid phase carrier, solid phase carrier is by with known benchmark as a comparison or quality-control sample and outer protective film in immune binding reagents layer, label, designation hole pearl or the pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93103933 CN1077288A (en) | 1993-04-01 | 1993-04-01 | Simplify the immunological method and the kit of fast detecting hepatitis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93103933 CN1077288A (en) | 1993-04-01 | 1993-04-01 | Simplify the immunological method and the kit of fast detecting hepatitis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1077288A true CN1077288A (en) | 1993-10-13 |
Family
ID=4984909
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 93103933 Pending CN1077288A (en) | 1993-04-01 | 1993-04-01 | Simplify the immunological method and the kit of fast detecting hepatitis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1077288A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100437113C (en) * | 2006-04-11 | 2008-11-26 | 汪运山 | Kit for detecting concentration ratio of phenacetin and acetaminophen in blood and application thereof |
| CN108196047A (en) * | 2017-12-28 | 2018-06-22 | 广东希格生物科技有限公司 | A kind of solid phase antibody double fastener heart immune response system and its kit and application |
-
1993
- 1993-04-01 CN CN 93103933 patent/CN1077288A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100437113C (en) * | 2006-04-11 | 2008-11-26 | 汪运山 | Kit for detecting concentration ratio of phenacetin and acetaminophen in blood and application thereof |
| CN108196047A (en) * | 2017-12-28 | 2018-06-22 | 广东希格生物科技有限公司 | A kind of solid phase antibody double fastener heart immune response system and its kit and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4242096A (en) | Immunoassay for antigens | |
| US4020151A (en) | Method for quantitation of antigens or antibodies on a solid surface | |
| US4067959A (en) | Indirect solid surface test for antigens or antibodies | |
| Dzantiev et al. | Electrochemical immunosensors for determination of the pesticides 2, 4-dichlorophenoxyacetic and 2, 4, 5-tricholorophenoxyacetic acids | |
| Suter | A modified ELISA technique for anti-hapten antibodies | |
| FI89984B (en) | ENVIRONMENTAL ASSESSMENT OF ANTIGEN SPECIFICATION ANTICROPHOTH IN THE IMMUNOGLOBULATION GROUP A, M, D ELLER E OCH MEDEL DAERFOER | |
| EP0125118A2 (en) | Colorimetric immunoassay on solid surface, dipstick therefor and its preparation | |
| AU645835B2 (en) | Simultaneous assay for detecting one or more analytes | |
| CA1256025A (en) | Immuno-chemical measurement process for haptens and proteins | |
| Aizawa | Immunosensors | |
| US4410634A (en) | Method of passively adsorbing immuno-reactive haptens to solid phases | |
| Yang et al. | Evaluation of enzyme-linked immunosorbent assay for the serodiagnosis of amebiasis | |
| CA1146853A (en) | Method of passively adsorbing immuno-reactive haptens to solid phases | |
| US5468618A (en) | Article for performing immunological assays utilizing organic dyes to immobilize immunologically reactive components to a solid phase support and methods for producing and utilizing same | |
| Litman et al. | An internally referenced test strip immunoassay for morphine. | |
| CN1077288A (en) | Simplify the immunological method and the kit of fast detecting hepatitis | |
| US4980299A (en) | Carrier for coating with immunologically-active material | |
| EP0231830A2 (en) | Method of immunochemical assay | |
| US5043288A (en) | Immobilize molecular binding partners to contact activating supports | |
| CN1402003A (en) | Reagent kit and use based on antigen-antibody reaction | |
| JPH0224559A (en) | Measurement of immunologically detectable substance, reactor and measurement of various parameters by method by immunoassay theory | |
| CA1146856A (en) | Method of detecting and quantitating haptens and antigens | |
| US4414324A (en) | Immunoassay method and apparatus | |
| USRE32696E (en) | Enzymatic immunological method for determination of antigens and antibodies | |
| Caldwell et al. | Enzyme immunoassay for hepatitis B and its comparison to other methods |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |