CN107727721A - The cholinesterase electrode being supported on using chitosan as binding agent in nickel foam - Google Patents
The cholinesterase electrode being supported on using chitosan as binding agent in nickel foam Download PDFInfo
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- CN107727721A CN107727721A CN201710777023.7A CN201710777023A CN107727721A CN 107727721 A CN107727721 A CN 107727721A CN 201710777023 A CN201710777023 A CN 201710777023A CN 107727721 A CN107727721 A CN 107727721A
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Abstract
The invention discloses a kind of cholinesterase electrode of the nickel foam as carrier to be filled with graphite, it is characterised in that：The acetylcholine esterase electrode using nickel foam/graphite as carrier includes nickel foam/graphite carrier layer and acetylcholinesterase working lining, and the preparation of the acetylcholine esterase electrode comprises the following steps：1) graphite, chitosan aqueous solution are mixed into the porous nickel mesh of filled therewith after treatment after porous nickel mesh is cleaned and dried, sheet is pressed into after drying, obtains nickel foam/graphite carrier layer；2) acetylcholinesterase working lining is prepared on nickel foam/graphite carrier layer, obtains the acetylcholine esterase electrode using nickel foam/graphite as carrier.The chitosan binding agent of the present invention used with cholinesterase electrode has the advantages that good biocompatibility, abundance nontoxic to human body and environment and without using organic solvent, the electrode has electrical conductivity height, specific surface area big, the features such as stability is good, detection sensitivity is high, available for the high-sensitivity detection to Organophosphorus and carbamate pesticides class pesticide molecule.
Description
Technical field
The present invention relates to electrochemica biological sensor field, and in particular to it is a kind of using be filled with the nickel foam of graphite as
The acetylcholine esterase electrode of carrier and its application in terms of Organophosphorus and carbamate pesticides class Pesticides Testing.
Background technology
Electrochemica biological sensor has high selectivity and sensitivity, energy quick response, and simple to operate, suitable agriculture
The Site Detection of medicine, its research are increasingly valued by people.
Nickel foam is a kind of ideal material for being used to prepare electrochemical sensor electrodes.Nickel foam is conductive can be excellent
Good, specific surface area is big and loose structure, it is easy to accomplish the features such as surface modification, is widely used in battery electrode material, electrochemistry
In terms of ultracapacitor, catalyst carrier, electrochemical electrode material.(shed is bright, Liu by Chinese patent ZL 201310042627.9
Bravely, Xue Yongliang, Yang Yan apple are using nickel foam as the cholinesterase electrode of carrier and application) disclose it is a kind of using nickel foam/graphite as
The cholinesterase electrode of carrier, but it uses oiliness binding agent, corresponding solvent in the preparation of nickel foam/graphite carrier
For organic solvent.Patent binding agent used in embodiment is cellulose acetate.Problems be present in cellulose acetate：
(1) organic solvent is used, not environmentally；(2) cellulose acetate solution is readily volatilized, and the viscosity in electrode matrix preparation process becomes
Change big；(3) the water-insoluble of cellulose acetate causes final cholinesterase electrode to be infiltrated in test fluid slowly, causes cholinester
The response speed of enzyme electrode is slow.The problem of existing for oil-soluble binding agent, the application consider to use aqueous binders such as shell
Glycan, hydroxypropyl methyl cellulose (HPMC) etc..In view of chitosan have good biocompatibility, it is nontoxic to human body and environment and
Its performance in the experiment of the application is better than HPMC, so the application chooses chitosan and is applied to cholinesterase as binding agent
The preparation of nickel foam/graphite matrix in electrode.
Organophosphorus and carbamate pesticides class agricultural chemicals has suppression to make to the normal function of acetylcholine ester enzymatic hydrolysis choline
With the concentration of its inhibiting rate and agricultural chemicals is proportionate.Simultaneously the hundred of acetylcholinesterase are calculated as follows in detection choline oxidation current
Divide inhibiting rate.
A=(I0-I)/I0× 100%
In formula:A refers to the percent inhibition of enzyme, I0It is stable to refer to the enzyme electrode not suppressed by organophosphor or carbamate pesticide
Response current (blank current), I refers to suppressed by organophosphor after enzyme electrode steady-state response electric current.
Theoretic Monitoring lower-cut can be defined as inhibiting rate (I when 10%10%) corresponding to inhibitor concentration.
The content of the invention
First purpose of the present invention is that provide one kind is supported on nickel foam/graphite carrier by binding agent of chitosan
Acetylcholine esterase electrode, the chitosan binding agent that the electrode uses have good biocompatibility, it is nontoxic to human body and environment,
Abundance and the advantages that without using organic solvent.The electrical conductivity of electrode is high, and the specific surface area of carrier is big, and stability is good, detection
High sensitivity, and preparation technology is simple, and cost is cheap, easily carries out chemical electrode modification.
Second object of the present invention is by the second being supported on using chitosan as binding agent on nickel foam/graphite carrier
Acetylcholinesterase electrode is used for the Electrochemical Detection of organophosphor or carbamate pesticide.
Technical scheme is illustrated below.
It is described using nickel foam as carrier the invention provides a kind of acetylcholine esterase electrode using nickel foam as carrier
Acetylcholine esterase electrode includes nickel foam/graphite carrier layer and acetylcholinesterase working lining, described using nickel foam as carrier
The preparation of acetylcholine esterase electrode comprise the following steps：
(1) porous nickel mesh is cleaned, drying process；It is filled in after graphite, chitosan and solvent are mixed into slurry
In porous nickel mesh after processing, sheet is pressed into after drying, obtains nickel foam/graphite carrier layer；With total matter of graphite and chitosan
It is 80%~99.5% to measure as 100% meter, content of graphite, and surplus is chitosan；The mass ratio of the chitosan and solvent is
0.1~5：100；The solvent is the acetic acid,diluted aqueous solution；
(2) acetylcholinesterase working lining is prepared on nickel foam/graphite carrier layer, obtained using nickel foam/graphite as load
The acetylcholine esterase electrode of body.
In step (1) of the present invention, the mass percent concentration of the acetic acid,diluted aqueous solution is 0.1~5%.The shell
Glycan is as binding agent.
In step (1) of the present invention, counted using the gross mass of graphite and binding agent as 100%, content of graphite be 84%~
99.5%, preferably 92%~99.5%, more preferably 96~99.5%.
The mass ratio of binding agent and solvent of the present invention is 0.1~5：100, preferably 0.5~3：100.
In step (2) of the present invention, the preparation of the acetylcholinesterase working lining uses conventional method.It is specific and
Speech, the acetylcholinesterase working lining can be prepared via a method which：By acetylcholine ester enzyme solutions and cross-linking agent solution with
And after protection agent solution mixing, be applied on nickel foam/graphite carrier layer, and make it that nickel foam/graphite carrier layer be completely covered, do
It is dry to obtain acetylcholinesterase working lining.Described crosslinking agent is preferably glutaraldehyde；Described protective agent is preferably chitosan.Institute
The acetylcholine ester enzyme solutions stated are to be dissolved in the phosphate buffer (PBS) of pH7.0 ± 0.5 by acetylcholinesterase and obtained
, wherein acetylcholinesterase mass concentration is preferably 0.05~0.2g/mL, more preferably 0.1g/mL；Described glutaraldehyde is molten
Liquid is to be dissolved in the phosphate buffer (PBS) of pH7.0 ± 0.5 by glutaraldehyde and obtained, wherein the mass percent of glutaraldehyde
Preferably 0.1~10%, preferably 2.5~10%, most preferably 5%；Described chitosan solution is that chitosan is dissolved in into 0.5
~5% (preferably 1~3%, obtain in acetic acid aqueous solution most preferably 1%), wherein chitosan mass concentration be preferably 0.5~
5%, more preferably 1~3%, most preferably 1%.The acetylcholine ester enzyme solutions, cross-linking agent solution, protect feeding intake for agent solution
Mass ratio is 1：0.025~0.1：0.05~2, preferably 1：0.025~0.1：0.5~2, most preferably 1：0.1：1.
Further, the acetylcholine esterase electrode using nickel foam as carrier also includes substrate layer, the substrate layer
It is connected by adhesive with nickel foam/graphite carrier layer.
Further, described substrate be inorganic material (such as glass, silica gel, aluminium, spot take off) or high polymer material (such as
Collagen, gelatin, chitosan, sodium alginate, polyvinyl chloride, nylon, nitrocellulose or cellulose acetate) prepare sheet material or
Net.
Further, the acetylcholine esterase electrode using nickel foam as carrier also includes electron mediator decorative layer,
The electron mediator decorative layer is located at nickel foam/between graphite carrier layer and acetylcholinesterase working lining；It is described with bubble
Foam nickel comprises the following steps for the preparation of the acetylcholine esterase electrode of carrier：
(a) porous nickel mesh is cleaned, drying process；It is filled in after graphite, chitosan and solvent are mixed into slurry
In porous nickel mesh after processing, sheet is pressed into after drying, obtains nickel foam/graphite carrier layer；With total matter of graphite and chitosan
It is 80%~99.5% to measure as 100% meter, content of graphite, and surplus is chitosan；The mass ratio of the chitosan and solvent is 0.1
~5：100；The solvent is the acetic acid,diluted aqueous solution；
(b) electron mediator decorative layer is prepared on nickel foam/graphite carrier layer；
(c) courage acetyl alkali esterase working lining is prepared on electron mediator decorative layer, is obtained using nickel foam/graphite as carrier
Acetylcholine esterase electrode.
The same above-mentioned steps of described step (a) (1), will not be repeated here.
In described step (b), the electron mediator contained in the electron mediator decorative layer is 7,7,8,8- tetra- cyanogen
One kind in base benzoquinones bismethane (TCNQ), ferrocene or derivatives thereof, the potassium ferricyanide etc..The electron mediator decorative layer
Preparation also uses conventional method, such as TCNQ decorative layers to be prepared via a method which：TCNQ is dissolved in acetone and obtains saturation
TCNQ solution, chitosan is dissolved in obtained in 0.5~5% acetic acid aqueous solution chitosan mass concentration be 0.5~5% shell gather
Sugar juice, then by saturation TCNQ solution and chitosan solution according to volume ratio 1:1 is well mixed, and mixed liquor is applied into foam
Nickel/graphite carrier layer surface, is dried to obtain TCNQ decorative layers；Further, the mass percent concentration of the acetic acid aqueous solution is excellent
Elect 1~3%, most preferably 1% as；The mass percent of chitosan is preferably 1~3% in the chitosan solution, most preferably
For 1%.
The preparation method of cholinesterase working lining also uses conventional method in described step (c), similar with step (2),
It will not be repeated here.
Further, described using nickel foam is the acetylcholine esterase electrode of carrier from top to down successively by acetylcholine
Esterase working lining, electron mediator decorative layer, nickel foam/graphite carrier layer and substrate layer composition, the preparation method of each layer are same as above.
Acetylcholine esterase electrode of the present invention using nickel foam/graphite as carrier can be applied to organophosphor or amino
The Electrochemical Detection of formic acid esters agricultural chemicals, described electro-chemical test use conventionally test solution, and test solution is moL/L containing 1m
Chlorinated thio acetylcholine and 0.1mol/L KCl 0.1mol/L phosphate buffer solutions (PBS) solution, described electrochemistry
Test is carried out under conditions of pH6.0~8.0, preferable ph 7.0.
If not present invention special instruction, the ratio of chemical reagent is all mass ratio in described technical recipe, described change
The percentage for learning reagent is all mass percent.
Compared with prior art, the present invention it is first public it is a kind of be used for electrochemica biological sensor in terms of using chitosan as
Binding agent is supported on the electrochemical electrode on nickel foam substrate, and its advantage is：1. use chitosan from the upper electrode is made
Binding agent has the advantages that good biocompatibility, abundance nontoxic to human body and environment and without using organic solvent；2. from property
The electrode, which can be gone up, has the features such as electrical conductivity is high, specific surface area is big, and stability is good, detection sensitivity is high, can be achieved to organophosphor
With the high-sensitivity detection of carbamate chemicals for agriculture molecule.
Brief description of the drawings
Fig. 1 is the overall structure figure using nickel foam/graphite as the acetylcholine ester electrode of carrier prepared in embodiment 1,
Wherein：1. enzyme layer, 2.TCNQ decorative layers, 3. nickel foams, 4.PVC plates.
Fig. 2 is that the acetylcholine esterase electrode using nickel foam/graphite as carrier prepared in embodiment 2 detects chlopyrifos
Standard curve.
Fig. 3 is the stability using nickel foam/graphite as the acetylcholine esterase electrode of carrier prepared in embodiment 3.
Fig. 4 is shadow of the graphite to acetylcholine esterase electrode oxidation peak current in cyclic voltammetry in embodiment 4
Ring.Wherein, abscissa content of graphite represents graphite and accounts for graphite and the percentage of chitosan gross mass, %.
Fig. 5 is that binding agent is to acetylcholine esterase electrode in cyclic voltammetry in embodiment 5 and comparative example 1,2
The influence of middle oxidation peak current.Wherein, abscissa binder content is that binding agent accounts for graphite and the percentage of binding agent gross mass
Than %.
Embodiment
Technical scheme is further described with reference to instantiation, but protection scope of the present invention is not
It is limited to this.
Embodiment 1：Preparation using nickel foam/graphite as the acetylcholine esterase electrode of carrier
1) cleaning of nickel foam/graphite carrier：Porous nickel mesh is cut into after 40 × 2mm size successively with hydrochloric acid, second
Alcohol, acetone, deionized water carry out ultrasonic wave cleaning, are then dried for standby；
2) it is graphite-filled：Taking graphite (500 mesh) and 3% chitosan solution, (ratio of graphite and chitosan is 98:2),
It is mixed into after slurry to be placed in crucible and is fully ground, by filled therewith in the ready porous nickel mesh of step 1, is done in fume hood
It is dry, under roll squeezer, it is pressed into the thin slice of 0.5mm thickness；Wherein
The configuration of 3% chitosan solution：It is fully more molten in the 0.5% acetic acid,diluted aqueous solution than adding chitosan into by metering
Solution, is positioned over standby in 4 DEG C of refrigerators.
3) prepared by substrate layer：Nickel foam/graphite carrier prepared by step 2 is connected to what is reduced in advance with adhesive
On PVC plates, PVC board size is consistent with nickel foam/graphite carrier size, and size is 40 × 2mm；
4) preparation of TCNQ decorative layers：After taking 50 μ L saturation TCNQ solution, 50 μ L1% chitosan solutions well mixed, 8 are taken
μ L drop coatings are in electrode surface；Wherein
The preparation of TCNQ saturated solutions：TCNQ is dissolved in the TCNQ that saturation untill it can not dissolve, is obtained in 2ml acetone
Solution；
5) acetylcholine ester enzyme layer coats：By 20 μ L acetylcholine ester enzyme solutions, the glutaraldehyde solutions of 2 μ L 5%, 20 μ L
1% chitosan solution, after mixing, drip on the electrode prepared in step 4, and make it that chitosan-TCNQ films be completely covered, be placed in room
Dried under temperature.The electrode prepared is dipped in the 0.02mol/L phosphate buffer solutions of pH 7.0, protected under the conditions of being placed in 4 DEG C
Deposit.Obtain the acetylcholine esterase electrode using nickel foam/graphite as carrier；Wherein
The preparation of the phosphate buffer solutions of pH 7.0：0.1mol/L Na is prepared respectively2HPO4With 0.1mol/L's
NaH2PO4Buffer solution, 0.1mol/L NaH is taken respectively2PO4Buffer solution 50ml, 0.1mol/L Na2HPO4Buffer solution 25ml, will
The two is mixed evenly, and adjusts pH value using two kinds of buffer solutions；
The preparation of 5% glutaraldehyde solution：25% glutaraldehyde solution 2ml is taken, is mixed with the 8ml phosphate buffer solutions of pH 7.0
Close, produce 5% glutaraldehyde solution；
The configuration of 0.1mg/ μ L acetylcholine ester enzyme solutions：100mg acetylcholinesterase freeze-dried powders are taken, are dissolved in 1000
μ l pH are in 7.0 phosphate buffer solution (PBS), are placed in 4 DEG C of refrigerators and preserve.
6) acetylcholine esterase electrode activity test method：The acetylcholine esterase electrode prepared is taken as work electricity
Pole, in the phosphate buffer solution (PBS, containing 0.10molL that pH values are 7.0-1KCl) in infiltration 30min after, using platinum electrode as
To electrode, Ag/AgCl is reference electrode, using the phosphate buffer solutions of the chlorinated thio acetylcholines of moL/L containing 1m (pH value as
7.0, KCl containing 0.1mol/L) it is test fluid, at room temperature with electrochemical workstation (U.S. Advanced Measurement
The conventional commercialization electrochemical apparatus of the company such as Technology, the defeated power of Britain is strong, Dutch ECO, Shanghai Chen Hua) measure chlorination second
The oxidation current of phatidylcholine (oxidizing potential is determined by the cyclic voltammogram of enzyme electrode in advance).
Embodiment 2：Acetylcholine esterase electrode is used for the Electrochemical Detection of organophosphorus pesticide
Step 1) is to step 6) with embodiment 1.
7) preparation of chlopyrifos standard sample：0.731g chlopyrifos pesticides original solutions are taken, are dissolved in 100ml's
1.2mmol/L acecolines phosphate buffer solution (pH7, containing 0.10molL-1KCl in), being configured to pesticide concentration is
1.0×10-2Mo1/L detects liquid, then is progressively diluted to pesticide concentration as 1.0 × 10-4mo1/L、1.0×10-5mo1/L、1.0×
10-6mo1/L、 1.0×10-7mo1/L、1.0×10-8mo1/L、1.0×10-9Mo1/L detection solution.
8) acetylcholine esterase electrode prepared is taken as working electrode, in the phosphate buffer solution that pH value is 7.0
(PBS, containing 0.10molL-1KCl in) after infiltration 30min, using platinum electrode as to electrode, Ag/AgCl is reference electrode, to add
The phosphate buffer solution (pH value 7.0, KCl containing 0.1mol/L) of 1m moL/L chlorinated thio acetylcholine is test fluid,
Current versus time curve of the acetylcholine esterase electrode under constant potential is determined with electrochemical workstation at room temperature, it is determined that necessarily
Current value as blank current value；Then after using deionized water rinsing electrode, acetylcholine esterase electrode is inserted and is configured with
The chlorinated thio acetylcholine cushioning liquid of agricultural chemicals, the current value after suppressing is measured, and the percentage that AChE is calculated as follows suppresses
Rate：
A=(I0-I)/I0× 100%
In formula:A refers to the percent inhibition of enzyme, I0Refer to enzyme electrode stable response electric current (the blank electricity not suppressed by agricultural chemicals
Stream), I refers to suppressed by agricultural chemicals after enzyme electrode steady-state response electric current.Theoretic Monitoring lower-cut can be defined as inhibiting rate 10%
When (I10%) corresponding to inhibitor concentration.
Standard curve (accompanying drawing 4) is made to the concentration of chlopyrifos with inhibiting rate, between the two linear is obtained by fitting
Equation is：I%=130.8+12.7lgC, coefficient R2=0.969, detection is limited to 4 × 10-11mo1/L。
The stability of the acetylcholine esterase electrode of embodiment 3
Preserved under the conditions of the acetylcholine esterase electrode prepared according to the method for embodiment 1 is placed in into 4 DEG C after placing 30 days,
According to the oxidation current of the method for embodiment 2 measure acecoline, response signal is original 88.3%.This shows the acetyl
Cholinesterase electrode has good stability.
Embodiment 4：Graphite accounts for the ratio of chitosan and graphite gross mass to carbon electrode electrochemistry on nickel foam/graphite carrier
The influence of performance
Step 1), step 3) to step 6) are with embodiment 1, step 7) and 8) with embodiment 2.It is different from embodiment 1
Change graphite in step 2) and add quality, make the mass percent of graphite and graphite in cellulose acetate gross mass be 84%,
88%th, 92%, 96%, 97%, 98%, 99%, 99.5%, obtain 8 carbon electrodes.
Embodiment 5：Chitosan accounts for the ratio of chitosan and graphite gross mass to carbon electrode electrification on nickel foam/graphite carrier
Learn the influence of performance
Step 1), step 3) to step 6) are with embodiment 1, step 7) and 8) with embodiment 2.It is different from embodiment 1
Change chitosan in step 2) and add quality, the mass percent for making graphite and chitosan in cellulose acetate gross mass is
0.5%th, 1%, 2%, 3%, 4%, obtain 5 carbon electrodes.
From figure 5 it can be seen that the binding agent of the electrode of two kinds of aqueous binders (chitosan and HPMC) has been used to use
Amount and oxidation spike potential are all substantially better than the electrode using cellulose acetate as binding agent, and in the contrast of two kinds of aqueous binders
In, chitosan is all substantially superior to HPMC in binding agent usage amount and oxidation spike potential.
The HPMC of comparative example 1 accounts for the ratio of HPMC and graphite gross mass to carbon electrode electrification on nickel foam/graphite carrier
Learn the influence of performance
Step 1), step 3) to step 6) are with embodiment 1, and step 7 and 8 is the same as embodiment 2.Difference is to make in step 2)
The graphite being filled in nickel foam is bonded with HPMC.Change graphite and add quality, make the matter of graphite and graphite in HPMC gross masses
It is 1%, 2%, 4%, 6%, 8% to measure percentage, obtains 5 carbon electrodes.
The compound method of HPMC binder solutions is：Take the 2/3 of 100mL deionized waters to be heated to 80 degrees Celsius, add
HPMC, stir while adding, remaining 1/3 cold water is slowly added to again after HPMC all dissolving, stirred while adding, slowly
It is cooled to room temperature.The mass fraction for taking HPMC is 2%, 4%, 6%, 8%.The viscosity of 8%HPMC binding agents is excessive, pulp flow
Property is poor.Take binder content be 4% and 6% when, the solid content most multipotency for adding graphite reaches 85%, because graphite solid content surpasses
Cross 85% slurry then prepared can excessively viscous flow it is poor, be unfavorable for the blade coating in later stage.Consider caking property and slurry
Mobility, final choice 2%HPMC.
The cellulose acetate of comparative example 2 accounts for the ratio of cellulose acetate and graphite gross mass to nickel foam/graphite carrier
The influence of upper carbon electrode chemical property
Step 1), step 3) to step 6) are with embodiment 1, and step 7 and 8 is the same as embodiment 2.Difference is in step 2)
The graphite being filled in nickel foam is bonded using cellulose acetate.Change graphite and add quality, make graphite total with cellulose acetate
The mass percent of graphite is 2%, 4%, 6%, 8%, 10% in quality, obtains 5 carbon electrodes.
The configuration of 6% cellulose acetate solution：By metering than cellulose acetate is added into 1:1 cyclohexanone and acetone
Mix in solvent, place 72h and be allowed to fully dissolve.
Claims (8)
- A kind of 1. acetylcholine esterase electrode using nickel foam/graphite as carrier, it is characterised in that：The acetylcholine esterase electrode using nickel foam/graphite as carrier includes nickel foam/graphite carrier layer and acetylcholine Esterase working lining, the preparation using nickel foam/graphite as the acetylcholine esterase electrode of carrier comprise the following steps：(1) porous nickel mesh is cleaned, drying process；Graphite, chitosan and solvent are mixed into after slurry and are filled in processing In porous nickel mesh afterwards, sheet is pressed into after drying, obtains nickel foam/graphite carrier layer；Using the gross mass of graphite and chitosan as 100% meter, content of graphite are 80%~99.5%, and surplus is chitosan；The mass ratio of the chitosan and solvent is 0.1~5： 100；The solvent is the acetic acid,diluted aqueous solution；(2) acetylcholinesterase working lining is prepared on nickel foam/graphite carrier layer, obtained using nickel foam/graphite as carrier Acetylcholine esterase electrode.
- 2. the acetylcholine esterase electrode using nickel foam/graphite as carrier as claimed in claim 1, it is characterised in that：It is described The acetylcholine esterase electrode using nickel foam/graphite as carrier also include substrate layer, the substrate layer passes through adhesive and bubble Foam nickel/graphite carrier layer connection.
- 3. the acetylcholine esterase electrode using nickel foam/graphite as carrier as claimed in claim 1, it is characterised in that：It is described The acetylcholine esterase electrode using nickel foam/graphite as carrier also include electron mediator decorative layer, the electron mediator Decorative layer is located at nickel foam/between graphite carrier layer and acetylcholinesterase working lining；It is described using nickel foam/graphite as carrier The preparation of acetylcholine esterase electrode comprise the following steps：(a) porous nickel mesh is cleaned, drying process；Graphite, chitosan and solvent are mixed into after slurry and are filled in processing In porous nickel mesh afterwards, sheet is pressed into after drying, obtains nickel foam/graphite carrier layer；Using the gross mass of graphite and chitosan as 100% meter, content of graphite are 80%~99.5%, and surplus is chitosan；The mass ratio of the chitosan and solvent is 0.1~5： 100；The solvent is the acetic acid,diluted aqueous solution；(b) electron mediator decorative layer is prepared on nickel foam/graphite carrier layer；(c) acetylcholinesterase working lining is prepared on electron mediator decorative layer, obtains the second using nickel foam/graphite as carrier Acetylcholinesterase electrode.
- 4. the acetylcholine esterase electrode using nickel foam/graphite as carrier as claimed in claim 3, it is characterised in that：It is described Electron mediator decorative layer in the electron mediator that contains be 7,7,8,8- four cyano benzoquinones bismethane, ferrocene or its derivative One kind in thing, the potassium ferricyanide.
- 5. the acetylcholine esterase electrode using nickel foam/graphite as carrier as claimed in claim 4, it is characterised in that：It is described Be the acetylcholine esterase electrode of carrier from top to down successively by acetylcholinesterase working lining, electronics using nickel foam/graphite Mediator decorative layer, nickel foam/graphite carrier layer and substrate layer composition.
- 6. the acetylcholine esterase electrode using nickel foam/graphite as carrier as claimed in claim 1, it is characterised in that：It is described The mass percent concentration of the acetic acid,diluted aqueous solution is 0.1~5%.
- 7. it is as claimed in claim 1 the acetylcholine esterase electrode of carrier in organophosphor or amino first using nickel foam/graphite Application in the Electrochemical Detection of acid esters agricultural chemicals.
- 8. application as claimed in claim 7, it is characterised in that：The Electrochemical Detection is entered under conditions of pH6.0~8.0 OK.
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| CN201710777023.7A CN107727721A (en) | 2017-09-01 | 2017-09-01 | The cholinesterase electrode being supported on using chitosan as binding agent in nickel foam |
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| CN201710777023.7A CN107727721A (en) | 2017-09-01 | 2017-09-01 | The cholinesterase electrode being supported on using chitosan as binding agent in nickel foam |
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| CN114252487A (en) * | 2020-09-24 | 2022-03-29 | 中国科学院理化技术研究所 | Enzyme electrode, electrochemical biosensor and method for analyzing phenol concentration in water |
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