CN107727598A - A kind of transmitted spectrum imaging method for aqueous hyaline tissue - Google Patents
A kind of transmitted spectrum imaging method for aqueous hyaline tissue Download PDFInfo
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- CN107727598A CN107727598A CN201710950127.3A CN201710950127A CN107727598A CN 107727598 A CN107727598 A CN 107727598A CN 201710950127 A CN201710950127 A CN 201710950127A CN 107727598 A CN107727598 A CN 107727598A
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- 238000001228 spectrum Methods 0.000 title claims abstract description 55
- 238000003384 imaging method Methods 0.000 title claims abstract description 25
- 210000004276 hyalin Anatomy 0.000 title claims abstract description 5
- 230000005540 biological transmission Effects 0.000 claims abstract description 64
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 8
- 230000003287 optical effect Effects 0.000 claims description 43
- 241001465754 Metazoa Species 0.000 claims description 20
- 238000000701 chemical imaging Methods 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 238000009826 distribution Methods 0.000 claims description 5
- 230000000704 physical effect Effects 0.000 claims description 5
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 241000894007 species Species 0.000 claims description 2
- 238000005286 illumination Methods 0.000 abstract description 6
- 238000001514 detection method Methods 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 239000000835 fiber Substances 0.000 description 11
- 230000003595 spectral effect Effects 0.000 description 9
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 238000005520 cutting process Methods 0.000 description 4
- 210000000582 semen Anatomy 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 230000004927 fusion Effects 0.000 description 3
- 235000014655 lactic acid Nutrition 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 238000009738 saturating Methods 0.000 description 3
- 210000002700 urine Anatomy 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 230000001795 light effect Effects 0.000 description 2
- 239000013307 optical fiber Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- GOLXNESZZPUPJE-UHFFFAOYSA-N spiromesifen Chemical compound CC1=CC(C)=CC(C)=C1C(C(O1)=O)=C(OC(=O)CC(C)(C)C)C11CCCC1 GOLXNESZZPUPJE-UHFFFAOYSA-N 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3581—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using far infrared light; using Terahertz radiation
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- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Toxicology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a kind of transmitted spectrum imaging method for aqueous hyaline tissue, this method includes the multispectral transmission calibration of laser, the calibration of Terahertz scanning transmission, the imaging of the multispectral transmission imaging of laser, Terahertz transmission scan, image calculating processing with building five, storehouse step.The beneficial effects of the invention are as follows; provide the illumination transmissive imaging method and Terahertz scanning lens imaging method in the even ray laser source of super continuous spectrums; combined standard transparency liquid sample corrects; the tissue signatures such as the structure of important living resources sample, texture, density, transmitance can be obtained; be advantageous to obtain the transmitted spectrum image containing much information, facilitate customs's inlet and outlet detection quarantine departments to carry out tracing to the source, differentiate and protecting for important living resources.
Description
Technical field
The present invention relates to a kind of transmission-type spectrum imaging method, more particularly to one kind to be shone using the even ray laser source of super continuous spectrums
The standard transmission-type imaging method of bright combination monochromatic light beam scanning THz source, suitable for the imaging point of the aqueous hyaline tissue of animals and plants
Analysis, belongs to photoelectronic imaging field.
Background technology
In inlet and outlet field, various countries mostly take the measure strictly controlled to the inlet and outlet of important living resources.Such as
Dong Zhi Quarantine Bureaus of United States Department of Agriculture provide, forbid the inhereditary materials such as domestic animal, hatching egg, animal semen, blood sample, embryo, excreta to go out
Immigration.In recent years, the animals and plants of Chinese exports are increased year by year, but original producton location identification, physical property and the something lost of current not yet Erecting and improving
Pass the metadatabases such as sign.Strengthen the protection to special strategic living resources and original producton location is traced to the source and studies and develop all kinds of something lost
The non-contact detection system for passing resource sample is imperative.
Physical property and heredity in the blade equal samples of rare animal blood, urine, seminal fluid and plant after lactic acid impregnates
In terms of characterizing metadatabase structure, high-resolution multispectral image can reflect that its structure, texture, transparency, form etc. are more
The feature of aspect, it is feasible efficient technological means.
The present invention use the even ray laser source of super continuous spectrums transmitted spectrum imaging method, for obtain rare animal blood,
The blade equal samples of urine, seminal fluid and plant after lactic acid impregnates visible and in the infrared multispectral standard transmitance figure of spectral coverage
Picture；Its Terahertz spectral coverage standard transmitance image is obtained using the method for monochromatic light beam scanning THz source.In the branch of two kinds of technologies
A kind of lower synthesis transmitted spectrum imager for the inlet and outlet protection of rare animals and plants of invention of support, the thing available for animal and plant resource
Property build storehouse and examination, facilitate customs's inlet and outlet detection quarantine departments to carry out important living resources and trace to the source, differentiate and protect.
The content of the invention
It is an object of the invention to provide the comprehensive transmission of a kind of imaging of standard super continuous spectrums and scanning terahertz imaging into
Image space method, the important living resources samples such as the blade of rare animal blood, urine, seminal fluid and plant after lactic acid impregnates can be obtained
Product it is visible, in infrared, Terahertz spectral coverage standard transmitance image, for important living resources build storehouse, trace to the source, differentiate and
Protection.
The present invention is achieved like this：
Integrated spectral imager proposed by the present invention is by embedded master controller, more Terahertz scanning imagery subsystem, light
Compose imaging subsystems, sample room and wireless lan transceiver composition；
Wherein Terahertz scanning imagery subsystem is by emitting head, reception head, bidimensional electric platforms and scanning and switching control
Device forms；Emitting head includes THz source and terahertz sources lens；Receiving head includes Terahertz receiving lens and Terahertz biography
Sensor；Emitting head is installed with receiving head by axis coaxle of Terahertz optical axis, and is fixed on bidimensional electric platforms；Bidimensional is electronic
Platform can do two dimensional surface scanning in the case where scanning the control with switch controller；
Multispectral imaging subsystem by optical filter wheel, optical filter wheel controller, bandpass filter, super continuous spectrums laser,
Fibre-coupled mirrors, one point of multi fiber, light uniforming device, secondary uniform light board, multispectral camera, multispectral imaging camera lens and the microscope group that is all-trans
Into；Multi-disc bandpass filter is housed on optical filter wheel, laser beam axis can be cut successively under the control of optical filter wheel controller；
After thering is sample cell, standard transparent liquid-like and switch, sample to put into sample cell in sample room, sample room pair is inserted
Answer notch；Switch controls switching standards transparency liquid sample or sample cell insertion sample room；Standard transparent liquid-like and sample cell
Planar dimension is consistent；
Super continuous spectrums laser sends the super continuous spectrums laser of the visible infrared spectral coverage into, is transmitted along laser beam axis, through light
After fine coupling mirror and a certain bandpass filter, export as the laser of a certain passband, be coupled into one point of multi fiber, carry out one to multiple point
Beam；Uniformly placement is distributed in light uniforming device plane the multi output optical fiber of one point of multi fiber；Light uniforming device plane is diffusing transmission plane, can
Make to obtain even light effect through light beam；Passband laser beam after even light reflects rear steering primary optical axis through total reflective mirror, then through secondary
After uniform light board, evenly, when its illumination is on the sample of sample room, its transmitted light passes through the light distribution that its surface is sent along primary optical axis
Multispectral imaging lens imaging is on multispectral camera；Laser beam axis is coplanar with primary optical axis, and laser beam axis is perpendicular to primary optical axis；
THz source can send the electromagnetic wave of terahertz wave band, can be converged at through terahertz sources lens along Terahertz optical axis
In the target of certain distance；Scanning in addition to it can control bidimensional electric platforms and do two dimensional surface scanning, is gone back with switch controller
It can control it to drive emitting head, receive head incision and cut out the spatial dimension of sample room, be workspace during incision, be now to work
State；It is nonclient area when cutting out, is now off working state；In working condition, terahertz sources lens will be along Terahertz
The certain point that the THz wave that optical axis transmits is converged on sample；Bidimensional electric platforms do two dimensional surface scanning, can be achieved
To sample plane THz wave a little focus on；The THz wave transmitted from sample THz wave focus point continues along terahertz
Hereby optical axis transmits, and by Terahertz receiving lens, converges at Terahertz sensor and is received；Both primary optical axis and Terahertz optical axis
It is coplanar；
Host software in embedded master controller can realize the man-machine interaction of instrument, database sharing, inquiry, long-range biography
It is defeated, the fusion of image information, analysis, taxonomic history；Its input/output port control program can be realized receives and dispatches to WLAN
Device, switch, optical filter wheel controller, THz source, scanning and switch controller, super continuous spectrums laser, multispectral camera
With the control of Terahertz sensor, and the output image of multispectral camera and the output on single point signal of Terahertz sensor are received,
And immigration department cloud system is connected by wireless lan transceiver network connection, realize that the upload, download and high in the clouds of database are looked into
Ask；
Comprehensive transmitted spectrum imaging method proposed by the present invention comprises the following steps：
(1) the multispectral transmission calibration of laser
Embedded master controller starts scanning and switch controller, and control bidimensional electric platforms drive emitting head, receive head
Into nonclient area, now Terahertz scanning imagery subsystem is off working state；Embedded main controller controls open super connect
Continuous spectrum laser and multispectral camera, start switch and standard transparent liquid-like are cut into sample room, start optical filter wheel control
First bandpass filter is cut laser beam axis by device, and now the transmitted light of the passband of standard transparent liquid-like first is imaged at most
Spectrum camera, then multispectral machine the first passband standards transmission image is mutually reached into embedded master controller；
After the standard transmission image acquisition for completing the first passband, embedded master controller starts optical filter wheel controller will
Second bandpass filter cuts laser beam axis, and now the transmitted light of the passband of standard transparent liquid-like second is imaged to multispectral
Camera, then multispectral machine the second passband standards transmission image is mutually reached into embedded master controller；So as to complete second
The acquisition of the standard transmission image of individual passband；
Similarly, be sequentially completed the three, the 4th ... until the laser light more of last passband standards transparency liquid sample
Spectrum standard transmission image obtains；
(2) Terahertz scanning transmission is calibrated
Embedded main controller controls close super continuous spectrums laser and multispectral camera；Embedded main controller controls are opened
Open THz source, Terahertz sensor, start scanning and switch controller, control bidimensional electric platforms drive emitting head, received
Head enters workspace, and now Terahertz scanning imagery subsystem is working condition；Scanning and switch controller control bidimensional are electronic
Platform drives emitting head, receives head progress two dimensional surface scanning, plane where its scanning range coverage criteria transparency liquid sample, sweeps
During retouching, Terahertz sensor gathers Terahertz transmitted wave signal point by point, and sends it to embedded master controller (note：
By the selection of two-dimensional scan step-length, the Pixel Dimensions and laser super continuous spectrums image pixel dimensions of Terahertz scan image are realized
Unanimously), the terahertz consistent with the multispectral standard transmission image pixel arrangement of above-mentioned laser is obtained by embedded master controller split
Hereby standard transmission image；
(3) the multispectral transmission imaging of laser
Embedded main controller controls close THz source and Terahertz sensor, embedded master controller start scanning with
Switch controller, control bidimensional electric platforms drive emitting head, reception head to enter nonclient area, now Terahertz scanning imagery
System is off working state；
Embedded master controller starts switch and standard transparent liquid-like is cut out into sample room；By certain rare animals and plants sample
After putting into sample cell, sample cell insertion sample room is corresponded to notch by switch；Embedded main controller controls open super continuous spectrums
Laser and multispectral camera, embedded master controller start optical filter wheel controller successively by first, second ..., it is straight
To last a piece of bandpass filter incision laser beam axis, meanwhile, multispectral camera obtains sample in each passband uniform laser successively
Transmission image under illumination, and it is sent to embedded master controller；
(4) Terahertz transmission scan is imaged
Embedded main controller controls close super continuous spectrums laser and multispectral camera；Embedded main controller controls are opened
Open THz source, Terahertz sensor, start scanning and switch controller, control bidimensional electric platforms drive emitting head, received
Head enters workspace, and now Terahertz scanning imagery subsystem is working condition；
Scanning drives emitting head, reception head to carry out two dimensional surface scanning with switch controller control bidimensional electric platforms, its
Plane where scanning range covering sample cell, in scanning process, Terahertz sensor gathers Terahertz transmitted wave signal point by point
(note：Now there is sample placement, therefore there will be absorption to THz wave when scanning element passes through sample, when scanning element falls in sample
When outside region, its transmission signal is maximum), and embedded master controller is sent it to, by embedded master controller split
Processing obtains the Terahertz transmission image of the sample consistent with above-mentioned Terahertz standard transmission image Pixel Dimensions；
(5) image calculates processing and builds storehouse
Embedded master controller is respectively by each passband standards transmission plot corresponding to each passband transmission image of sample divided by its
Picture, so as to obtain each passband standards transmitance image (i.e. super continuous spectrums standard transmitance image) of sample；Similarly, it is embedded
The Terahertz transmission image of sample divided by its Terahertz standard transmission image are obtained Terahertz standard transmitance figure by master controller
Picture；The super continuous spectrums of the rare animals and plants sample and Terahertz standard transmitance image and its kind, the place of production, tissue etc. are believed
Breath converges, and builds the physical property resource databases such as its biological structure, texture, aqueous distribution, and the database information of the sample is led to
Cross wireless lan transceiver network and deliver to entry and exit department cloud system；Substantial amounts of rare animals and plants sample is completed based on the instrument
After database sharing, unknown sample is detected with the instrument, after obtaining its super continuous spectrums and Terahertz standard transmitance image,
Retrieved, contrasted, identified with rare animals and plants sample database, you can carry out the place of production trace to the source, Species estimation etc., so as to effectively
Important living resources protection is carried out, safeguards national bio-safety.
The invention has the advantages that provide the illumination transmissive imaging method and terahertz in the even ray laser source of super continuous spectrums
Hereby scanning lens imaging method, the correction of combined standard transparency liquid sample, can obtain the structure of important living resources sample, texture,
The tissue signatures such as density, transmitance, be advantageous to obtain the transmitted spectrum image containing much information, facilitate customs's inlet and outlet detection
Quarantine departments carry out tracing to the source, differentiate and protecting for important living resources.
Brief description of the drawings
Fig. 1 is present system structural representation, in figure：1 --- embedded master controller；2 --- Terahertz is scanned into
As subsystem；3 --- scanning and switch controller；4 --- THz source；5 --- emitting head；6 --- Terahertz optical axis；
7 --- terahertz sources lens；8 --- sample room；9 --- sample cell；10 --- sample；11 --- secondary equal tabula rasa；
12 --- primary optical axis；13 --- receive head；14 --- optical filter wheel；15 --- optical filter wheel controller；16 --- bandpass filter
Piece；17 --- laser beam axis；18 --- super continuous spectrums laser；19 --- fibre-coupled mirrors；20 --- one point of multi fiber；
21 --- light uniforming device；22 --- multispectral camera；23 --- multispectral imaging subsystem；24 --- multispectral imaging camera lens；
25 --- total reflective mirror；26 --- wireless lan transceiver；27 --- workspace；28 --- nonclient area；29 --- Terahertz
Sensor；30 --- bidimensional electric platforms；31 --- standard transparent liquid-like；32 --- switch；33 --- Terahertz receives
Lens.
Embodiment
The specific embodiment of the invention is as shown in Figure 1.
Integrated spectral imager proposed by the present invention is by embedded master controller 1, Terahertz scanning imagery subsystem 2, more
Light spectrum image-forming subsystem 23, sample room 8 and wireless lan transceiver 26 form；
Wherein Terahertz scanning imagery subsystem 2 by emitting head 5, receive first 13, bidimensional electric platforms 30 and scanning with cutting
Changer controller 3 forms；Emitting head 5 includes THz source 4 and terahertz sources lens 7；Receiving first 13 includes Terahertz reception thoroughly
Mirror 33 and Terahertz sensor 29；Emitting head 5 is installed with receiving first 13 with Terahertz optical axis 6 for axis coaxle, and is fixed on two
Tie up on electric platforms 30；Bidimensional electric platforms 30 can do two dimensional surface scanning in the case where scanning the control with switch controller 3；
Multispectral imaging subsystem 23 is by optical filter wheel 14, optical filter wheel controller 15, bandpass filter 16, super continuous spectrums
It is laser 18,19, one points of multi fibers 20 of fibre-coupled mirrors, light uniforming device 21, secondary uniform light board 11, multispectral camera 22, multispectral
Imaging lens 24 form with total reflective mirror 25；Equipped with (the note of multi-disc bandpass filter 16 on optical filter wheel 14：The present embodiment is 6, will
400-2500nm spectral coverages are divided into 6 passbands), laser beam axis 17 can be cut successively under the control of optical filter wheel controller 15；
After thering is sample cell 9, standard transparent liquid-like 31 and switch 32, sample 10 to put into sample cell 9 in sample room 8, insert
Enter 8 corresponding notch of sample room；Switch 32 controls switching standards transparency liquid sample 31 or sample cell 9 to insert sample room；Standard is saturating
Prescribed liquid sample 31 is consistent with the planar dimension of sample cell 9；
(the note of super continuous spectrums laser 18：Its spectral region of the present embodiment 400-2500nm, mean power 2W) send it is visible
Into, the super continuous spectrums laser of infrared spectral coverage, is transmitted along laser beam axis 17, through fibre-coupled mirrors 19 and a certain bandpass filter 16
Afterwards, export as the laser of a certain passband, be coupled into one point of multi fiber 20, carry out one to multiple beam splitting (note：The present embodiment uses one point
Ten six fiberses)；Uniformly placement is distributed in the plane of light uniforming device 21 the multi output optical fiber of one point of multi fiber 20；The plane of light uniforming device 21 is
Diffusing transmission plane, it can make to obtain even light effect through light beam；Passband laser beam after even light reflects rear steering master through total reflective mirror 25
Optical axis 12, then through secondary uniform light board 11 after, the light distribution that its surface is sent evenly, when its illumination sample room 8 sample
On 10, its transmitted light is imaged onto on multispectral camera 22 along primary optical axis 12 through multispectral imaging camera lens 24；Laser beam axis 17 and master
Optical axis 12 is coplanar, and laser beam axis 17 is perpendicular to primary optical axis 12；
THz source 4 can send the electromagnetic wave of terahertz wave band, can through terahertz sources lens 7 along Terahertz optical axis 6
Gather in the target of certain distance；Scanning is swept with switch controller 3 except bidimensional electric platforms 30 can be controlled to do two dimensional surface
Retouch outer, also can control it to drive emitting head 5, receive spatial dimensions of first 13 incision with cutting out sample room, be workspace during incision
27, it is now working condition；It is nonclient area 28 when cutting out, is now off working state；In working condition, terahertz sources
The certain point that lens 7 converge at the THz wave transmitted along Terahertz optical axis 6 on sample 10；Bidimensional electric platforms 30 are done
Two dimensional surface scan, can be achieved to the plane of sample 10 institute a little THz wave focusing；It is saturating from the THz wave focus point of sample 10
The THz wave of injection continues to transmit along Terahertz optical axis 6, by Terahertz receiving lens 33, converges at Terahertz sensor 29
Received；Both primary optical axis 12 and Terahertz optical axis 6 are coplanar；
Host software in embedded master controller 1 can realize the man-machine interaction of instrument, database sharing, inquiry, long-range
Transmission, the fusion of image information, analysis, taxonomic history；Its input/output port control program can be realized to be received to WLAN
Send out device 26, switch 32, optical filter wheel controller 15, THz source 4, scanning and switch controller 3, super continuous spectrums laser
18th, the control of multispectral camera 22 and Terahertz sensor 29, and receive the output image and Terahertz biography of multispectral camera 22
The output on single point signal of sensor 29, and immigration department cloud system is connected by the network of wireless lan transceiver 26 connection, realize number
Inquired about according to the upload, download and high in the clouds in storehouse；
Comprehensive transmitted spectrum imaging method proposed by the present invention comprises the following steps：
(1) the multispectral transmission calibration of laser
Embedded master controller 1 starts scanning and switch controller 3, and control bidimensional electric platforms 30 drive emitting head 5, connect
Receive first 13 and enter nonclient area 28, now Terahertz scanning imagery subsystem 2 is off working state；Embedded master controller 1 is controlled
System opens super continuous spectrums laser 18 and multispectral camera 22, starts switch 32 and standard transparent liquid-like 31 is cut into sample
Room, start optical filter wheel controller 15 and first bandpass filter 16 is cut into laser beam axis 17, now standard transparent liquid-like
The transmitted light of 31 first passbands is imaged to multispectral camera 22, and then multispectral machine phase 22 is by the first passband standards transmission plot
As reaching embedded master controller 1；
After the standard transmission image acquisition for completing the first passband, embedded master controller 1 starts optical filter wheel controller
Second bandpass filter 16 is cut laser beam axis 17 by 15, now the transmitted light of the passband of standard transparent liquid-like 31 second by into
The second passband standards transmission image is reached embedded master controller 1 by picture to multispectral camera 22, then multispectral machine phase 22；
So as to complete the acquisition of the standard transmission image of second passband；
Similarly, be sequentially completed the three, the 4th ... until last passband (note：The present embodiment is 6 passbands) mark
The multispectral standard transmission image of laser of quasi- transparency liquid sample 31 obtains；
(2) Terahertz scanning transmission is calibrated
Super continuous spectrums laser 18 and multispectral camera 22 are closed in the embedded control of master controller 1；Embedded master controller 1
(the note of THz source 4 is opened in control：Be 2.5THz Terahertzs cascaded laser in the present embodiment), Terahertz sensor 29, start
Scanning and switch controller 3, control bidimensional electric platforms 30 drive emitting head 5, reception first 13 to enter workspace 27, now terahertz
Hereby scanning imagery subsystem 2 is working condition；Scanning controls bidimensional electric platforms 30 to drive emitting head 5, connect with switch controller 3
Receive first 13 and carry out two dimensional surface scanning, its place plane of scanning range coverage criteria transparency liquid sample 31, in scanning process, terahertz
Hereby sensor 29 gathers Terahertz transmitted wave signal point by point, and sends it to embedded master controller 1 (note：Swept by two dimension
The selection of step-length is retouched, realizes that the Pixel Dimensions of Terahertz scan image are consistent with laser super continuous spectrums image pixel dimensions, this reality
It is 2560*1920 to apply an image resolution ratio), obtained by the embedded split of master controller 1 saturating with the multispectral standard of above-mentioned laser
Penetrate image pixel and arrange consistent Terahertz standard transmission image；
(3) the multispectral transmission imaging of laser
THz source 4 and Terahertz sensor 29 are closed in the embedded control of master controller 1, and embedded master controller 1 starts
Scanning and switch controller 3, control bidimensional electric platforms 30 drive emitting head 5, reception first 13 to enter nonclient area 28, now too
Hertz scanning imagery subsystem 2 is off working state；
Embedded master controller 1 starts switch 32 and standard transparent liquid-like 31 is cut out into sample room；By certain rare dynamic plant
After thing sample 10 puts into sample cell 9, sample cell 9 is inserted 8 corresponding notch of sample room by switch 32；Embedded master controller 1 is controlled
System opens super continuous spectrums laser 18 and multispectral camera 22, and embedded master controller 1 starts optical filter wheel controller 15 successively
By first, second ..., until last a piece of bandpass filter 16 cuts laser beam axis 17, meanwhile, multispectral camera 22
Transmission image of the sample 10 under the illumination of each passband uniform laser is obtained successively, and is sent to embedded master controller 1；
(4) Terahertz transmission scan is imaged
Super continuous spectrums laser 18 and multispectral camera 22 are closed in the embedded control of master controller 1；Embedded master controller 1
Control opens THz source 4, Terahertz sensor 29, starts scanning and switch controller 3, and control bidimensional electric platforms 30 drive
Emitting head 5, first 13 are received into workspace 27, now Terahertz scanning imagery subsystem 2 is working condition；
Scanning controls bidimensional electric platforms 30 to drive the first 13 progress two dimensional surface of emitting head 5, reception to sweep with switch controller 3
Retouch, its scanning range covering place plane of sample cell 9, in scanning process, Terahertz sensor 29 gathers Terahertz transmission point by point
Ripple signal (note：Now there is sample 10 to place, therefore there will be absorption to THz wave when scanning element passes through sample, work as scanning element
When falling outside sample area, its transmission signal is maximum), and embedded master controller 1 is sent it to, by embedded master control
The split of device 1 processed handles to obtain the Terahertz transmission plot of the sample 10 consistent with above-mentioned Terahertz standard transmission image Pixel Dimensions
Picture；
(5) image calculates processing and builds storehouse
Embedded master controller 1 respectively transmits each passband standards corresponding to each passband transmission image of sample 10 divided by its
Image, so as to obtain each passband standards transmitance image (i.e. super continuous spectrums standard transmitance image) of sample 10；Similarly, it is embedding
Enter formula master controller 1 by the Terahertz transmission image of sample 10 divided by its Terahertz standard transmission image, obtain Terahertz standard
Transmitance image；By the super continuous spectrums of the rare animals and plants sample 10 and Terahertz standard transmitance image and its kind, production
The information fusions such as ground, tissue, the physical property resource databases such as its biological structure, texture, aqueous distribution are built, and by the sample 10
Database information delivers to entry and exit department cloud system by the network of wireless lan transceiver 26；Completed based on the instrument substantial amounts of
After rare animals and plants sample database structure, unknown sample is detected with the instrument, obtains its super continuous spectrums and Terahertz mark
After quasi- transmitance image, retrieved, contrasted, identified with rare animals and plants sample database, you can the progress place of production is traced to the source, kind
Identification etc., so as to effectively carry out important living resources protection, safeguard national bio-safety.
Claims (1)
1. a kind of transmitted spectrum imaging method for aqueous hyaline tissue, this method is real on a kind of transmitted spectrum imager
Existing, the transmitted spectrum imager is by embedded master controller (1), Terahertz scanning imagery subsystem (2), multispectral imaging
Subsystem (23), sample room (8) and wireless lan transceiver (26) composition；It is characterized in that transmitted spectrum imaging method includes
Following steps：
1) the multispectral transmission calibration of laser
Embedded master controller starts scanning and switch controller, and control bidimensional electric platforms drive emitting head, reception head to enter
Nonclient area, now Terahertz scanning imagery subsystem is off working state；Embedded main controller controls open super continuous spectrums
Laser and multispectral camera, start switch and standard transparent liquid-like is cut into sample room, starting optical filter wheel controller will
First bandpass filter cuts laser beam axis, and now the transmitted light of the passband of standard transparent liquid-like first is imaged to multispectral
Camera, then multispectral machine the first passband standards transmission image is mutually reached into embedded master controller；
After the standard transmission image acquisition for completing the first passband, embedded master controller starts optical filter wheel controller by second
Piece bandpass filter cuts laser beam axis, and now the transmitted light of the passband of standard transparent liquid-like second is imaged to multispectral phase
Machine, then multispectral machine the second passband standards transmission image is mutually reached into embedded master controller；So as to complete second
The acquisition of the standard transmission image of passband；
Similarly, be sequentially completed the three, the 4th ... until the multispectral mark of laser of last passband standards transparency liquid sample
Quasi- transmission image obtains；
2) Terahertz scanning transmission is calibrated
Embedded main controller controls close super continuous spectrums laser and multispectral camera；Embedded main controller controls are opened too
Hertz source, Terahertz sensor, start scanning and switch controller, control bidimensional electric platforms drive emitting head, reception head to enter
Enter workspace, now Terahertz scanning imagery subsystem is working condition；Scanning and switch controller control bidimensional electric platforms
Drive emitting head, receive head progress two dimensional surface scanning, plane where its scanning range coverage criteria transparency liquid sample is scanned
Cheng Zhong, Terahertz sensor gathers Terahertz transmitted wave signal point by point, and sends it to embedded master controller, by embedded
Master controller split obtains the Terahertz standard transmission image consistent with the multispectral standard transmission image pixel arrangement of above-mentioned laser；
By the selection of two-dimensional scan step-length, the Pixel Dimensions and laser super continuous spectrums image pixel dimensions of Terahertz scan image are realized
Unanimously；
3) the multispectral transmission imaging of laser
Embedded main controller controls close THz source and Terahertz sensor, and embedded master controller starts scanning and switching
Controller, control bidimensional electric platforms drive emitting head, reception head to enter nonclient area, now Terahertz scanning imagery subsystem
For off working state；
Embedded master controller starts switch and standard transparent liquid-like is cut out into sample room；Certain rare animals and plants sample is put into
After sample cell, sample cell insertion sample room is corresponded to notch by switch；Embedded main controller controls open super continuous spectrums laser
Device and multispectral camera, embedded master controller start optical filter wheel controller successively by first, second ..., until most
A piece of bandpass filter incision laser beam axis afterwards, meanwhile, multispectral camera obtains sample and illuminated in each passband uniform laser successively
Under transmission image, and be sent to embedded master controller；
4) Terahertz transmission scan is imaged
Embedded main controller controls close super continuous spectrums laser and multispectral camera；Embedded main controller controls are opened too
Hertz source, Terahertz sensor, start scanning and switch controller, control bidimensional electric platforms drive emitting head, reception head to enter
Enter workspace, now Terahertz scanning imagery subsystem is working condition；
Scanning drives emitting head, reception head to carry out two dimensional surface scanning with switch controller control bidimensional electric platforms, and it is scanned
Plane where scope covering sample cell, in scanning process, Terahertz sensor gathers Terahertz transmitted wave signal point by point, now has
Sample is placed, therefore will have absorption to THz wave when scanning element passes through sample, when scanning element falls outside sample area, its
Transmission signal is maximum, and sends it to embedded master controller, by embedded master controller split handle to obtain with it is upper
State the Terahertz transmission image of the consistent sample of Terahertz standard transmission image Pixel Dimensions；
5) image calculates processing and builds storehouse
Embedded master controller respectively by each passband transmission image of sample divided by its corresponding to each passband standards transmission image, from
And obtain each passband standards transmitance image of sample, i.e. super continuous spectrums standard transmitance image；Similarly, embedded main control
The Terahertz transmission image of sample divided by its Terahertz standard transmission image are obtained Terahertz standard transmitance image by device；Will
The information such as the super continuous spectrums and Terahertz standard transmitance image and its kind, the place of production, tissue of the rare animals and plants sample are converged
Close, build the physical property resource databases such as its biological structure, texture, aqueous distribution, and the database information of the sample is passed through into nothing
Line local area network transceiver network delivers to entry and exit department cloud system；Substantial amounts of rare animals and plants sample data is completed based on the instrument
After the structure of storehouse, unknown sample is detected with the instrument, after obtaining its super continuous spectrums and Terahertz standard transmitance image, with treasure
Dilute animals and plants sample database is retrieved, contrasted, identified, you can the progress place of production is traced to the source, Species estimation.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109580534A (en) * | 2018-11-30 | 2019-04-05 | 深圳市太赫兹科技创新研究院有限公司 | Substance classes recognition methods, terahertz time-domain spectroscopy instrument system and terminal device |
| CN111091030A (en) * | 2018-10-24 | 2020-05-01 | 中国测绘科学研究院 | Tree species identification method and device, computer equipment and readable storage medium |
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| CN105675549A (en) * | 2016-01-11 | 2016-06-15 | 武汉大学 | Portable crop parameter measurement and growth vigor intelligent analysis device and method |
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