CN107703134B - Kit and method for detecting ammonia nitrogen content in water body and application - Google Patents
Kit and method for detecting ammonia nitrogen content in water body and application Download PDFInfo
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- CN107703134B CN107703134B CN201711071770.5A CN201711071770A CN107703134B CN 107703134 B CN107703134 B CN 107703134B CN 201711071770 A CN201711071770 A CN 201711071770A CN 107703134 B CN107703134 B CN 107703134B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/3103—Atomic absorption analysis
Abstract
The invention relates to a kit and a method for detecting ammonia nitrogen content in a water body and application. The kit comprises: the reagent comprises a NasLei reagent, a low-concentration boric acid solution of 1.5-2.5 g/L and a high-concentration boric acid solution of 15-25 g/L. The kit, the method and the application respectively use the boric acid solutions with different concentrations to absorb water samples with different ammonia nitrogen concentrations, ensure that the ammonia nitrogen content in the water samples can be accurately detected by adjusting the pH value of the distillation products to be neutral after the boric acid solutions are used to absorb the pre-distillation products, simplify the operation and simultaneously ensure the accuracy of the detection result.
Description
Technical Field
The invention relates to the field of water body detection, in particular to a kit and a method for detecting the ammonia nitrogen content of a water body and application thereof.
Background
Ammonia nitrogen refers to free ammonia (NH) in water3) And ammonium ion (NH)4 +) Nitrogen in the form present. The composition ratio of the two is determined by the pH value and the water temperature of water, and the content of free ammonia and the pH value form a positive relation and the water temperature form a reverse relation; the ammonium salt is the opposite. The ammonia nitrogen in water mainly comes from three aspects: the interconversion among urban domestic sewage, ammonia and nitrite and some industrial waste water, such as coking waste water and waste water of synthetic ammonia fertilizer plant.
Ammonia nitrogen is a nutrient in water, can cause water eutrophication, is a main oxygen-consuming pollutant in the water and is toxic to fishes and some aquatic organisms. Therefore, ammonia nitrogen is a routine monitoring project for water environment and wastewater, is one of key indexes for evaluating water quality safety, and is important for monitoring and treating in the integrated wastewater discharge standard (GB8978-1996) and the surface water environment quality standard (GB 3838-2002). The method can quickly and accurately measure the ammonia nitrogen in the water, and is a precondition for correctly evaluating the water environment quality and further formulating water pollution prevention measures.
The nano-reagent spectrophotometry is a method for measuring the content of ammonia nitrogen in air and water by an ultraviolet-visible spectrophotometry, wherein the reaction principle is as follows: iodine ions and mercury ions react with ammonia under the strong alkaline condition to generate a light reddish brown complex, the color can be strongly absorbed at the wavelength of 420nm, the absorbance of the generated reddish brown complex is in direct proportion to the ammonia nitrogen content of the solution of the complex, and the ammonia nitrogen content can be determined by testing the absorption value of the reaction solution.
The nano-reagent spectrophotometry is used as a conventional monitoring method for measuring ammonia nitrogen in water, has the characteristics of simple and convenient operation, sensitive reaction, rapidness, high efficiency and the like, is widely applied to all levels of environmental monitoring departments, and is listed as the industry standard of the department of environmental protection (the nano-reagent spectrophotometry for measuring ammonia nitrogen in water HJ 535-2009).
However, in the actual monitoring work, when ammonia nitrogen in a water sample is detected by adopting a nano-reagent spectrophotometry, the water sample with color or dirtier needs to be pre-distilled, the pH value after distillation can obviously influence the test result, and the data is easy to distort.
According to the national standard method, after the pH value of the sample subjected to pre-distillation treatment is adjusted to be neutral, the experimental requirement of low-concentration ammonia nitrogen cannot be met, the result is low, and the standard addition recovery rate is low. Most scholars adjust the pH of a sample to 9-13, and experiments show that the experiment requirements can be met only by accurately adjusting the pH, so that the difficulty is high. The scholars also think that the test sample after the pre-distillation treatment is diluted to reduce the influence of the boric acid matrix on the color development, but the method is only suitable for water samples with larger ammonia nitrogen content, and when the ammonia nitrogen content of the water samples is smaller, the dilution multiple influences the test result of the ammonia nitrogen.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The kit comprises a low-concentration boric acid solution and a high-concentration boric acid solution which are respectively used for absorbing a pre-distillation product of a low-concentration ammonia nitrogen water body and a pre-distillation product of a high-concentration ammonia nitrogen water body, the pH of the pre-distillation product absorbed by the boric acid solution is not required to be accurately adjusted, the pH can be directly detected by using a Nashin reagent only by adjusting the pH to be neutral, the operation is convenient, and the detection result is accurate and reliable.
The second purpose of the invention is to provide a method for measuring the ammonia nitrogen content in a water body, before using a Nashiner reagent for detection, boric acid solutions with different concentrations are respectively used for absorbing the pre-distilled distillation product, the pH value of the distillation product does not need to be accurately adjusted, and the Nashiner reagent can be used for detection only by adjusting to be neutral, so that the operation is simplified, and the accurate and reliable detection result is ensured.
The third purpose of the invention is to provide the application of the method in water quality assessment or wastewater pollution monitoring, and the application can conveniently and accurately detect the content of ammonia nitrogen in the water body so as to conveniently assess the water quality and monitor the wastewater pollution.
In order to achieve the above purpose of the present invention, the following technical solutions are adopted:
a kit for detecting the ammonia nitrogen content of a water body comprises: the reagent comprises a NasLei reagent, a low-concentration boric acid solution of 1.5-2.5 g/L and a high-concentration boric acid solution of 15-25 g/L.
A method for determining ammonia nitrogen content in a water body, comprising the following steps:
(A) pre-distilling the water body to be detected, estimating the ammonia nitrogen content of the water body to be detected, if the ammonia nitrogen content of the water body is between 0.1 and 90.6mg/L, absorbing the distillation product by using a boric acid solution with the concentration of 1.5 to 2.5g/L, and if the ammonia nitrogen content of the water body is more than 90.6mg/mL, absorbing the distillation product by using a boric acid solution with the concentration of 15 to 25 mg/L;
(B) adjusting the pH value of the distillation product to be neutral, carrying out color reaction on the distillation product absorbed by the boric acid solution by using a Nashiner reagent, and obtaining the ammonia nitrogen content of the water body to be detected according to the absorbance value.
The method is applied to water quality assessment or wastewater pollution monitoring.
Detailed Description
The invention relates to a kit for detecting ammonia nitrogen content in a water body, which comprises: the reagent comprises a NasLei reagent, a low-concentration boric acid solution of 1.5-2.5 g/L and a high-concentration boric acid solution of 15-25 g/L.
The conventional kit for detecting the ammonia nitrogen content in the water body only comprises a high-concentration boric acid solution, does not comprise a low-concentration boric acid solution, a pre-distillation product is still absorbed by the low-concentration ammonia nitrogen water body by adopting the high-concentration boric acid solution of 20g/L, the boric acid in the pre-distillation product which is easily absorbed by the high-concentration boric acid is obviously excessive, the excessive boric acid reacts with a Navier reagent, the Navier equilibrium is moved leftwards, the result is low or even not detected, the accuracy of ammonia nitrogen content detection is greatly influenced, the ammonia nitrogen content can be accurately detected only by adjusting the pH value of a sample to 9-13, and the operation method is very complicated.
The kit disclosed by the invention not only comprises a Nashin reagent and 15-25 g/L of high-concentration boric acid solution, but also comprises 1.5-2.5 g/L of low-concentration boric acid solution, wherein the low-concentration boric acid solution is used for absorbing a pre-distillation product of low-concentration ammonia nitrogen water body, and ammonia gas and NaOH can completely react boric acid in the solution without causing interference on the color development of the Nashin reagent after the pH of a distilled sample is adjusted to be neutral. Therefore, the color development test can be directly carried out by adjusting the PH value of the sample to be neutral, the operation steps of ammonia nitrogen concentration measurement can be greatly simplified, and the detection accuracy is ensured.
In some specific embodiments, the low-concentration boric acid solution has a concentration of 1.6g/L, 1.7g/L, 1.8g/L, 1.9g/L, 2.0g/L, 2.1g/L, 2.2g/L, 2.3g/L, 2.4g/L, or 2.5 g/L.
In some specific embodiments, the high-concentration boric acid solution has a concentration of 16g/L, 17g/L, 18g/L, 19g/L, 20g/L, 21g/L, 22g/L, 23g/L, 24g/L, or 25 g/L.
In some specific embodiments, the kit further comprises one or more of a standard color chart, an ammonia nitrogen standard stock solution, non-ammonia water, a masking agent, a chlorine removal agent, a pH adjusting agent, a pH indicator, light magnesium oxide, and a starch-potassium iodide test paper.
In some specific embodiments, the masking agent is selected from one or more of potassium sodium tartrate, sodium hexametaphosphate, sodium acetate, sodium malate, and tetrasodium EDTA; the dechlorinating agent is sodium thiosulfate; the pH regulator is hydrochloric acid or sodium hydroxide solution; the pH indicator is a bromothymol blue indicator.
The invention also relates to a method for measuring the ammonia nitrogen content of the water body, which comprises the following steps:
(A) pre-distilling the water body to be detected, estimating the ammonia nitrogen content of the water body to be detected, if the ammonia nitrogen content of the water body is between 0.1 and 90.6mg/L, absorbing the distillation product by using a boric acid solution with the concentration of 1.5 to 2.5g/L, and if the ammonia nitrogen content of the water body is more than 90.6mg/mL, absorbing the distillation product by using a boric acid solution with the concentration of 15 to 25 mg/L;
(B) adjusting the pH value of the distillation product to be neutral, carrying out color reaction on the distillation product absorbed by the boric acid solution by using a Nashiner reagent, and obtaining the ammonia nitrogen content of the water body to be detected according to the absorbance value.
Different from the conventional detection method, the method disclosed by the invention is used for estimating the ammonia nitrogen content of the water sample to be detected before detection, the high-ammonia nitrogen content water sample to be detected is absorbed by using a high-concentration boric acid solution, the low-ammonia nitrogen concentration water sample to be detected is absorbed by using a low-concentration boric acid solution, and the absorbed pre-distillation product is adjusted to be neutral so as to carry out subsequent color reaction by using a Nashiner reagent. The method can ensure the detection accuracy without adjusting the pH of the water sample to be detected with low ammonia nitrogen concentration to 9-13, and has the advantages of simplifying the operation steps of ammonia nitrogen concentration measurement and simultaneously ensuring the detection accuracy.
In some specific embodiments, if the ammonia nitrogen content of the water body is between 0.1 and 50mg/L, a boric acid solution with a concentration of 1.6g/L, 1.7g/L, 1.8g/L, 1.9g/L, 2.0g/L, 2.1g/L, 2.2g/L, 2.3g/L, 2.4g/L or 2.5g/L is used for absorbing the distillation product;
if the ammonia nitrogen content of the water body is more than 50mg/L, a boric acid solution with the concentration of 16g/L, 17g/L, 18g/L, 19g/L, 20g/L, 21g/L, 22g/L, 23g/L, 24g/L or 25g/L is used for absorbing the distillation product.
In some specific embodiments, the method further comprises diluting the water to be tested before the pre-distillation treatment, preferably by 10-1000 times, such as 50 times, 100 times, 150 times, 200 times, 250 times, 300 times, 350 times, 400 times, 450 times, 500 times, 550 times, 600 times, 650 times, 700 times, 750 times, 800 times, 850 times, 900 times, 950 times or 1000 times; or the distillation product after absorption with boric acid is diluted after the preliminary distillation treatment, preferably at a dilution factor of 10 to 1000 times, for example, 50 times, 100 times, 150 times, 200 times, 250 times, 300 times, 350 times, 400 times, 450 times, 500 times, 550 times, 600 times, 650 times, 700 times, 750 times, 800 times, 850 times, 900 times, 950 times, or 1000 times.
In some specific embodiments, the method further comprises pretreating the water sample to be tested with a chlorine removal agent before the pre-distillation treatment; preferably, the chlorine removal agent is sodium thiosulfate; more preferably, the pretreatment further comprises using a starch-potassium iodide test paper to detect whether chlorine in the water sample to be detected is completely removed.
In some embodiments, the method further comprises the step of adding a masking agent while performing the color reaction.
In some specific embodiments, the masking agent is selected from one or more of potassium sodium tartrate, sodium hexametaphosphate, sodium acetate, sodium malate, and tetrasodium EDTA; preferably, the masking agent is potassium sodium tartrate.
In some embodiments, the water body is selected from one or more of a river, a lake, groundwater, a reservoir water sample, domestic drinking water, industrial wastewater, or domestic wastewater.
The invention also relates to the application of the method in evaluating water quality or monitoring wastewater pollution.
Compared with the prior art, the invention has the beneficial effects that:
(1) according to the kit and the method, boric acid solutions with different concentrations are respectively used for absorbing water samples with different ammonia nitrogen concentrations, so that after the boric acid solution is used for absorbing the pre-distilled product, the pH value of the distilled product is adjusted to be neutral, the ammonia nitrogen content in the water sample can be accurately detected, the operation is simplified, and meanwhile, the accuracy of the detection result is ensured.
(2) The kit and the method also relate to a masking agent and a chlorine removing agent, wherein the masking agent and the chlorine removing agent can be used for further removing the influence of chlorine and metal ions on the detection result and increasing the accuracy of the detection result.
Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by manufacturers, and are all conventional products available on the market.
An experimental instrument:
TU1900 ultraviolet double-beam spectrophotometer, distillation apparatus (Shengtai science and technology), ultra-pure water apparatus, 500ml all-glass distillation flask.
Experimental reagent:
ammonia nitrogen standard stock solution (1000mg/L), ammonia nitrogen standard use solution (10mg/L) and potassium sodium tartrate solution (500 g/L); naeser reagent (HgI)2-KI-NaOH); boric acid absorption liquid (2g/L), boric acid absorption liquid (20 g/L); light magnesium oxide (heated at 500 ℃ for two hours to remove carbonates); sodium thiosulfate solution (3.5 g/L); hydrochloric acid (1.18 g/mL); NaOH (250 g/L); bromothymol blue indicator (0.5 g/L); starch-potassium iodide test paper.
Example 1
Detect the ammonia nitrogen concentration of power plant desulfurization waste water 1#, power plant desulfurization waste water 2#, reservoir sewage 3#, reservoir sewage 4 #:
1. plotting a calibration curve
(1) Sucking standard ammonium use solution of 0ml, 0.50 ml, 1.00 ml, 2.00 ml, 4.00 ml, 6.00 ml, 8.00 ml and 10.00ml into a colorimetric tube of 50ml, adding water to dilute until the mark line is formed, adding 1.0ml of potassium sodium tartrate solution, and mixing uniformly;
(2) adding 1.0m L Nhr's reagent (HgI)2-KI-NaOH), mixing, standing for 10min, measuring the absorbance of the standard use solution at a wavelength of 420nm by using a cuvette with a light path of 20mm and taking ammonia-free water as a reference;
(3) and subtracting the absorbance of the zero-concentration blank from the measured absorbance to obtain corrected absorbance, and drawing a calibration curve of ammonia nitrogen content (mu g) to the corrected absorbance. The equation for the calibration curve obtained in this example is 0.00337x-0.00069, and R is 0.9998.
2. Sample pretreatment
(1) Elimination of residual chlorine
The reaction result is influenced when residual chlorine exists in the water quality sample, so that a proper amount of sodium thiosulfate solution is added into the 1# water sample, the 2# water sample, the 3# water sample and the 4# water sample to eliminate interference.
In order to test whether residual chlorine is removed completely, starch-potassium iodide test paper is used for synchronously testing: and (3) dripping the treated water sample on a starch-potassium iodide test paper, wherein if the test paper is changed into blue, the water quality sample is not completely treated, and residual chlorine still exists, and if the test paper is not discolored, the water quality sample is completely treated, and the residual chlorine is completely treated.
(2) Step of adding a label
Respectively taking 50ml of the No. 1 water sample and 50ml of the No. 2 water sample in a 500ml flask, and adding water to dilute to 250 ml;
preparing a No. 1 standard water sample and a No. 2 standard water sample which are parallel to the No. 1 water sample and the No. 2 water sample: respectively taking 50ml of the No. 1 water sample and 50ml of the No. 2 water sample, respectively adding 100ml of 1000mg/L ammonium chloride standard solution, and adding water to 250 ml;
respectively taking 200ml of No. 3 and No. 4 samples in a 500ml flask;
preparing a 3# water sample and a 4# water sample which are parallel to the 3# water sample and the 4# water sample: the sample amount of the added standard sample is also 200ml, 20ml of 10mg/L ammonium chloride transition liquid is added, and water is added to 250 ml.
(3) Preliminary distillation
For a 1# water sample, a 2# water sample, a 1# standard water sample and a 2# standard water sample, the boric acid absorption liquid is a 20g/L boric acid solution, and for a 3# water sample, a 4# water sample, a 3# standard water sample, a 4# standard water sample and an ammonia nitrogen quality control sample 200566#, the boric acid absorption liquid is a 2g/L boric acid solution.
The 50ml of boric acid absorbent was transferred to a 250ml volumetric flask ensuring the condenser outlet below the boric acid solution level.
Taking 250ml samples (respectively No. 1 water sample, No. 2 water sample, No. 1 standard water sample, No. 2 standard water sample, No. 3 water sample, No. 4 water sample, No. 3 standard water sample, No. 4 standard water sample and ammonia nitrogen quality control sample 200566#), transferring into a flask, adding a few drops of bromothymol blue indicator, adjusting the pH to 6.0 (the indicator is yellow) to 7.4 (the indicator is blue) by using sodium hydroxide solution or hydrochloric acid solution if necessary, adding 0.25g of light magnesium oxide and a plurality of glass beads, and immediately connecting a nitrogen ball and a condenser pipe. Heating and distilling to make the velocity of distillate about 10ml/min, stopping distilling when the distillate reaches 200ml, adjusting pH to neutral, and fixing volume to 250 ml.
For a 1# water sample, a 2# water sample, a 1# standard water sample and a 2# standard water sample, after distillation, the pH value of the samples is adjusted to be neutral, and after dilution by 500 times, 50ml of the samples are added with a color developing agent for testing; for a 3# water sample, a 4# water sample, a 3# standard water sample and a 4# standard water sample, adjusting the pH value of the samples to be neutral after distillation, and directly sampling 50ml and adding a color developing agent for testing; for ammonia nitrogen quality control sample No. 200566, after distillation, the pH value of the sample is adjusted to be neutral and diluted by 25 times, and then a sample is directly taken and added with a color developing agent for testing.
3. Color reaction of water sample
(1) Respectively taking a diluted No. 1 water sample, a diluted No. 2 water sample, a No. 1 standard water sample, a No. 2 standard water sample and an ammonia nitrogen quality control sample No. 200566, and an undiluted No. 3 water sample, a No. 4 water sample, a No. 3 standard water sample, a No. 4 standard water sample and ammonia-free water, adding the obtained mixture to the marked line of a 50ml colorimetric tube, adding 1.0ml of a sodium potassium tartrate solution, and uniformly mixing.
(2) Adding 1.0mL of Nashi reagent, mixing, standing for 10min, and measuring absorbance at a wavelength of 420nm by using a cuvette with a light path of 20mm, wherein ammonia-free water is used as a blank sample to make a blank in the whole process.
(3) And subtracting the absorbance of the blank sample from the absorbance measured by the water sample to obtain corrected absorbance, substituting the corrected absorbance into the equation of the calibration curve to obtain the ammonia nitrogen concentration of the water sample, wherein the specific ammonia nitrogen concentration of the 1# to 4# water sample is shown in table 1, and the specific standard adding recovery result of the 1# to 4# standard adding water sample is shown in table 2.
According to the results shown in tables 1-2, the relative deviation of the 1# to 4# water sample and the recovery rate of the 1# to 4# standard water sample can meet the standard requirements, and the quality control sample 200566 is in the range of 30.0 +/-1.7 mg/L, which shows that the improved method can meet the requirements of experiments and simplify the experimental steps.
TABLE 1 Ammonia Nitrogen concentration of Water samples
TABLE 2 spiking recovery experiment
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (9)
1. A method for measuring the ammonia nitrogen content of a water body is characterized by comprising the following steps:
(A) pre-distilling the water body to be detected, estimating the ammonia nitrogen content of the water body to be detected, if the ammonia nitrogen content of the water body is between 0.1 and 90.6mg/L, absorbing the distillation product by using a boric acid solution with the concentration of 1.5 to 2.5g/L, and if the ammonia nitrogen content of the water body is more than 90.6mg/L, absorbing the distillation product by using a boric acid solution with the concentration of 15 to 25 g/L;
(B) adjusting the pH value of the distillation product to be neutral, carrying out color reaction on the distillation product absorbed by the boric acid solution by using a Nashiner reagent, and obtaining the ammonia nitrogen content of the water body to be detected according to the absorbance value.
2. The method according to claim 1, further comprising diluting the water to be measured by 10-1000 times before the pre-distillation treatment; or diluting the distillation product after the distillation treatment with boric acid, wherein the dilution multiple is 10-1000 times.
3. The method according to claim 1, further comprising pre-treating the water sample to be tested with a chlorine removal agent prior to the pre-distilling treatment.
4. The method of claim 3, wherein the chlorine scavenger is sodium thiosulfate.
5. The method of claim 3, wherein the pre-treating further comprises using a starch-potassium iodide test strip to detect the removal of chlorine from the water sample to be tested.
6. The method of claim 1, further comprising the step of adding a masking agent while performing the color reaction.
7. The method of claim 6, wherein the masking agent is selected from one or more of potassium sodium tartrate, sodium hexametaphosphate, sodium acetate, sodium malate, and tetrasodium EDTA.
8. The method according to any one of claims 1 to 7, wherein the water body is selected from one or more of rivers, lakes, underground water, reservoir water samples, domestic drinking water, industrial wastewater or domestic wastewater.
9. Use of the method of any one of claims 1 to 6 for assessing water quality or monitoring wastewater pollution.
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| CN110441469A (en) * | 2019-09-19 | 2019-11-12 | 威海长青海洋科技股份有限公司 | A kind of water body ammonia nitrogen detection kit and detection method |
| CN111781198A (en) * | 2020-07-15 | 2020-10-16 | 南通大学 | Two-dimensional determination method for ammonia nitrogen content in water body, soil or sediment |
| CN111912800B (en) * | 2020-08-11 | 2023-07-18 | 深圳市泛邦建设工程咨询有限公司 | Method for detecting low-concentration ammonia nitrogen in drinking water |
| CN112834495A (en) * | 2021-01-04 | 2021-05-25 | 广东环凯生物技术有限公司 | Test paper for rapidly detecting ammonia nitrogen in water, preparation method and detection method thereof |
| CN113607725A (en) * | 2021-07-30 | 2021-11-05 | 云南铜业股份有限公司西南铜业分公司 | Method for determining ammonia nitrogen content in wastewater |
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