CN107699485A - Microelectrode fluidic chip and adjustable parameter single cell electroporation device - Google Patents
Microelectrode fluidic chip and adjustable parameter single cell electroporation device Download PDFInfo
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- CN107699485A CN107699485A CN201711082795.5A CN201711082795A CN107699485A CN 107699485 A CN107699485 A CN 107699485A CN 201711082795 A CN201711082795 A CN 201711082795A CN 107699485 A CN107699485 A CN 107699485A
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- microelectrode
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
Abstract
The invention discloses a kind of microelectrode fluidic chip and adjustable parameter single cell electroporation device, microelectrode fluidic chip includes transparency carrier and microfluidic channel layer, microfluidic channel layer is located above transparency carrier, microelectrode is integrated with transparency carrier, microelectrode includes interdigital electrode and parallel pole, interdigital electrode is with parallel distribution of electrodes in the lower section of micro-fluidic electroporation passage, and interdigital electrode and parallel pole are sequentially distributed from cell suspending liquid entrance to cell outlet.Adjustable parameter single cell electroporation device includes power module of voltage regulation, module, PCB control circuits and digital display module, chip carrier and microelectrode fluidic chip occur for function signal.The present invention can realize carries out electroporation processing successively to individual cells;Reduce the cellular damage even death rate;Improve cell electrotransfection efficiency;The species and quantity of access electrode are selected by control circuit, changes the frequency, amplitude, dutycycle of electric signal, the optimization of Electroporation parameters is realized for different cell lines.
Description
Technical field
The invention belongs to microflow control technique and single cell electroporation, electrotransfection field, more particularly to one kind to be based on microelectrode
The adjustable parameter single cell electroporation device of fluidic chip.
Background technology
Microflow control technique (Microfluidics) is in a few micrometers to hundreds of microns of fluid channel processing or behaviour using size
Vertical volume is the Science and Technology involved by nanoliter system for arriving microlitre fluid, is one and is related to micro-nano technology, physics, micro- electricity
The emerging cross discipline in the fields such as sub-, biology, chemistry, new material.Based on the chip of microflow control technique to be miniaturized, integrate
Feature is turned to, generally again by title chip lab (Lab on a Chip) and micro-total analysis system (microTAS).Micro-fluidic skill
Art is considered as having huge development potentiality in biomedical research and is widely applied prospect.
, it is necessary to apply the electricity in the external world, optical signalling when carrying out biological, chemical sample detection and analysis with micro-fluidic chip
Excitation reflects the parameter of sample to be tested by output electricity, optical signalling.Especially, for electrical detection analytical technology
For, it need to utilize micro-processing technology Integrated electrode on micro-fluidic chip, be interacted with realizing with outer signals, so that in miniflow
Control and electrical detection method is integrated on chip.The micro-fluidic chip of integrated microelectrode possesses many superior performances:Detected in manipulation
While biological specimen, electric field caused by microelectrode can promote cell to produce some physiological reactions, such as perforate, crack, or will
Associated electrical information (such as resistance antinoise signal) feeds back to experimental system to realize cell detection, and possesses high sensitivity, response
The advantages that fast and the potentiality of device miniaturization.
Cell electroporation technology (Electroporation) is also known as electrotransfection technology, is commonly used in cell transfection technique
A kind of approach.Because cell membrane has selective penetrated property to external substance, control gene of eucaryote cell experiment needs thin to eucaryon
Specific biological DNA, RNA fragment of born of the same parents' input.Apply the electrical potential difference of some strength in cell membrane both sides and continue for some time, carefully
Micropore just can be produced on after birth, strengthens membrane passage.When electroporation occurs for cell membrane, its permeability and membrane conductance meeting
Instantaneous increase, so that hydrophilic molecules, DNA, protein, virion, drug particles etc. can not pass through cell under normal circumstances
The molecule of film gets enter into cell.After removing electrical potential difference in a short time, cell membrane can self-recovery, again turn into selectivity
Permeability barrier.Compared with traditional chemical transfection and virus transfection, electroporation has more wide applicability and superiority:It is applicable
In plasmid and tens KB genomic fragment, without chemical viral pollution, acellular permanent damage, transient transfection the advantages that.Cause
This, electroporation technology has broad application prospects in fields such as biophysics, molecular biology, clinical medicine.
Electrode size is big in traditional cell electroporation instrument, electrode spacing still in macro-size, in ten mm-scales,
And cell size is in micro-meter scale, therefore the voltage that need to apply is big, about hundreds of volts, and electric field is uneven, each cell
Residing electric field environment is different, causes that the cell close to electrode is easily dead, and the cell at compared with weak electric field can not be worn again
Hole transfects, and survival rate and transfection efficiency are all than relatively low.The parameters such as existing micro-fluidic electroporation device electrode spacing are fixed, experiment
Operability and Electroporation parameters controllability by chip design limited, lack versatility.In addition, commercialized electricity
The key parameters such as signal frequency, the amplitude of instrument are transfected all by factory settings, client can not optimize regulation, for special
Cell, such as primary cell, immunocyte transfection efficiency be not high.
The content of the invention
Goal of the invention:In order to solve the problems, such as that prior art is present, electroporation device is set to be common to various cells, the present invention
A kind of microelectrode fluidic chip and adjustable parameter single cell electroporation device is provided.
Technical scheme:A kind of microelectrode fluidic chip, including transparency carrier and microfluidic channel layer, microfluidic channel layer position
Above transparency carrier, the microfluidic channel layer includes sheath inflow entrance, cell suspending liquid entrance, sheath circulation road, cell and suspended
Liquid passage, micro-fluidic electroporation passage and cell outlet;Sheath inflow entrance connects with sheath circulation road, cell suspending liquid entrance and cell
Suspension passage is connected, and the end of sheath circulation road is converged with cell suspending liquid channel end and with the one of micro-fluidic electroporation passage
End connection, the other end of micro-fluidic electroporation passage connect with cell outlet;Microelectrode, micro- electricity are integrated with the transparency carrier
Pole includes interdigital electrode and parallel pole, and interdigital electrode, in the lower section of micro-fluidic electroporation passage, and is pitched with parallel distribution of electrodes
Refer to electrode and parallel pole is sequentially distributed from cell suspending liquid entrance to cell outlet;Microelectrode includes microelectrode exit, micro-
Electrode leads to client is distributed in transparency carrier both sides.
Preferably, the interdigital electrode and/or parallel pole have multiple and uniform intervals to be distributed.Electric Field Distribution can be made more
Add uniformly, make the electric field environment residing for each cell close, improve survival rate and transfection efficiency.
Preferably, the sheath circulation road includes two passage tributaries, and two passage tributaries are respectively from cell suspending liquid entrance
Both sides connect around cell suspending liquid entrance with cell suspending liquid passage, and the sheath stream in two passage tributaries can be effectively by cell
The flowing of microchannel center is converged in, realizes and electroporation processing is carried out successively to individual cells.
Preferably, in addition to cell suspending liquid input pipe, sheath stream input pipe, cell efferent duct, cell suspending liquid input pipe
Connected with cell suspending liquid entrance, sheath stream input pipe connects with sheath inflow entrance, and cell efferent duct connects with cell delivery outlet.
Preferably, transparency carrier is provided with bonding cross alignment mark, and microfluidic channel layer is provided with being bonded with ten
Four square alignment marks of the bonding that word alignment mark is engaged, bonding make transparency carrier be combined more with microfluidic channel layer
Accurately, with ensure electrode be able to be located at micro-fluidic electroporation passage in.
Preferably, the finger spacing of interdigital electrode is 50~80 μm;The electrode spacing of parallel pole is 150~200 μm.
A kind of adjustable parameter single cell electroporation device using microelectrode fluidic chip, including power module of voltage regulation, letter
Number signal generating module, PCB control circuits and digital display module, chip carrier and microelectrode fluidic chip, chip carrier are provided with
Chip slot, the microelectrode fluidic chip are fixed in chip slot, and PCB control circuits and digital display module output end are visited for spring
Pin, the spring probe make electrical contact with microelectrode exit;Power module of voltage regulation is used to module, PCB controls occur for function signal
Circuit processed and digital display module and the power supply of microelectrode fluidic chip;Function signal occurs module and is used to believe needed for output power supply perforation
Number.
Preferably, the PCB control circuits and digital display module include on-off circuit and amplifier, and on-off circuit is defeated by single channel
Enter and be divided into multiple-channel output, amplifier is used for the amplitude for adjusting output signal;The input contiguous function signal of on-off circuit occurs
The output end of module, the input of the output end connection amplifier of on-off circuit;The output end connection microelectrode of amplifier is drawn
End.
Preferably, amplifier is gain adjustable amplifier.
Preferably, interdigital electric field of the interdigital electrode is more than the electric field between electrodes of parallel pole.
Beneficial effect:A kind of microelectrode fluidic chip provided by the invention, compare prior art, microelectrode fluidic chip
In microelectrode include interdigital electrode and parallel pole, interdigital electrode is located at micro-fluidic electroporation passage upstream, produces highfield,
Cell suspending liquid by when by electric field action, electroporation occurs for cell membrane;Parallel pole is located under micro-fluidic electroporation passage
Trip, applies and keeps existing fringing field, can maintain cell electroporation state, strengthen and improve the transfection efficiency of cell.Each cell
Residing electric field environment is close, and cellular damage caused by reducing conventional electroporation instrument highfield and electric-field intensity difference is even dead
Die.
Adjustable parameter single cell electroporation device based on microelectrode fluidic chip, compare prior art, and 1) device
For complete single cell electroporation experimental system, it is convenient to carry out single cell electroporation experiment, be placed on inverted microscope
Observe the dynamic process of cell electroporation;2) device uses microelectrode fluidic chip, and spacing is short between electrode, to reach cell electricity
The effect of perforation and the voltage that applies is low in 10V magnitudes, cell mortality, safe operation;3) device to individual cells successively
Electroporation is carried out, and the electric field environment residing for each cell is close, reduces cell caused by uncontrollable factor in experimentation and damages
Wound;4) electrode geometrical parameter of the core microelectrode fluidic chip of the device can be designed according to requirement of experiment, and electricity is controlled by PCB
Road selects the species of electrode used in electroporation and number and selects waveform, frequency, dutycycle and the amplitude of electric signal,
Realize the optimization regulation of electroporation experiment parameter;5) the microelectrode fluidic chip of the device designs simultaneously in microfluidic channel downstream
Parallel pole is machined with, existing fringing field is produced when cell flows through the region, effectively enhances cell electrotransfection efficiency.
Brief description of the drawings
Fig. 1 is the three dimensional structure diagram of microelectrode fluidic chip in the present invention;
Fig. 2 is the three dimensional structure diagram of microfluidic channel layer in microelectrode fluidic chip in the present invention;
Fig. 3 is the transparency carrier three dimensional structure diagram for integrating microelectrode in the present invention in microelectrode fluidic chip;
Fig. 4 is microelectrode fluidic chip A-A profile structure diagram;
Fig. 5 is the structured flowchart of the adjustable parameter single cell electroporation device of the present invention.
Embodiment
The invention will be further described with specific embodiment below in conjunction with the accompanying drawings.
As shown in figure 1, microelectrode fluidic chip includes transparency carrier 10 and microfluidic channel layer 20, microfluidic channel layer 20
Above transparency carrier 10.As shown in Fig. 2 the microfluidic channel layer 20 includes sheath inflow entrance 201, cell suspending liquid entrance
202nd, sheath circulation road 203, cell suspending liquid passage 204, micro-fluidic electroporation passage 205 and cell outlet 206;Sheath inflow entrance
201 connect with sheath circulation road 203, and cell suspending liquid entrance 202 connects with cell suspending liquid passage 204, the end of sheath circulation road 203
End is converged with the end of cell suspending liquid passage 204 and connected with one end of micro-fluidic electroporation passage 205, and micro-fluidic electroporation leads to
The other end in road 205 connects with cell outlet 206.As shown in figure 3, it is integrated with microelectrode, microelectrode on the transparency carrier 10
Including interdigital electrode 101 and parallel pole 102, interdigital electrode 101 and parallel pole 102 are distributed in micro-fluidic electroporation passage
205 lower section, micro-fluidic electroporation passage 205 is just from interdigital electrode 101 and parallel pole 102 from the point of view of vertical view
Between pass through, the two-stage of electrode is located at the micro-fluidic both ends of electroporation passage 205 respectively, and interdigital electrode 101 and parallel pole 102 from
Cell suspending liquid entrance 202 is sequentially distributed to cell outlet 206, i.e. interdigital electrode 101 close to cell suspending liquid entrance 202,
Upstream;Parallel pole 102 is close to cell outlet 206, in downstream;Microelectrode includes microelectrode exit 103, microelectrode exit
103 are distributed in the both sides of transparency carrier 10.
Interdigital electrode 101 in the present embodiment has three, and parallel pole 102 has one, can actually be arranged as required to
Corresponding number, parallel pole 102 can also have multiple.Interval is uniformly distributed between multiple microelectrodes, designs phase as needed
The spacing of adjacent microelectrode, Electric Field Distribution can be made more uniform, make the electric field environment residing for each cell close, improve survival rate
And transfection efficiency.Microelectrode spacing is embodied in geometric electrode size and arrangement when making transparency carrier 10 and designed.
The sheath circulation road 203 includes two passage tributaries, and two passage tributaries are respectively from 202 liang of cell suspending liquid entrance
Side connects around cell suspending liquid entrance 202 with cell suspending liquid passage 204, the sheath stream in two passage tributaries can effectively by
Cell converges in the flowing of microchannel center, realizes and carries out electroporation processing successively to individual cells.
As shown in figure 4, also include sheath stream input pipe 301, cell suspending liquid input pipe 302, cell efferent duct 303, cell
Suspension input pipe 302 connects with cell suspending liquid entrance 202, and sheath stream input pipe 301 connects with sheath inflow entrance 201, and cell is defeated
Outlet pipe 303 connects with cell delivery outlet 206, to facilitate cell suspending liquid, the injection of sheath stream and the outflow of mixing liquid.Sheath stream
Input pipe 301, cell suspending liquid input pipe 302, cell efferent duct 303 typically use teflon pipe, it is possible to use stainless steel tube,
Specific diameter can determine according to the cell suspending liquid entrance 202, sheath inflow entrance 201, the aperture of cell outlet 206.
Transparency carrier 10 is provided with bonding cross alignment mark 104, and microfluidic channel layer 20 is provided with being bonded with ten
Four square alignment marks 207 of the bonding that word alignment mark 104 is engaged, i.e., can also using sphere of movements for the elephants shape alignment mark
It is the same using the alignment mark of other shapes, effect.Sheath inflow entrance 201, cell suspending liquid entrance are additionally provided with transparency carrier 10
202nd, the processing alignment mark 105 of cell outlet 206.Bonding shares four directions with lucky sunk key at cross-shaped alignment marks 104
In shape alignment mark 207, transparency carrier 10 is set to be combined with microfluidic channel layer 20 more accurate, to ensure that micro-fluidic electroporation leads to
Road 205 be able to be located in microelectrode.In the occasion without fine registration, bonding alignment mark can not be also designed.
Spacing between the two end electrodes of microelectrode can be designed as needed, the interdigital electrode 101 of the present embodiment
Finger spacing be 50~80 μm;The electrode spacing of parallel pole 102 is 150~200 μm.Both changeabilities are embodied in transparent base
Electrode parameter design when plate 10 designs.
As shown in figure 5, use the adjustable parameter single cell electroporation device of the microelectrode fluidic chip, including voltage-stabilized power supply
Module, PCB control circuits and digital display module, chip carrier and microelectrode fluidic chip, chip carrier occur for module, function signal
Chip slot is provided with, the microelectrode fluidic chip is fixed in chip slot, and PCB control circuits and digital display module output end are bullet
Spring probe, the spring probe make electrical contact with microelectrode exit.
Power module of voltage regulation is used to module, PCB control circuits and digital display module and microelectrode stream control occur for function signal
Chip power supply.
Module, which occurs, for function signal can control the parameters such as the waveform for being applied to microelectrode power on signal, frequency, dutycycle,
For exporting perforation desired signal of powering.
PCB control circuits and digital display module can be applied to microelectrode power on signal by gain adjustable amplifier to adjust
Amplitude, realize for different cell lines Electroporation parameters optimization regulation.The PCB control circuits and digital display module bag
On-off circuit and amplifier are included, single channel input is divided into multiple-channel output by on-off circuit;Amplifier is used for the width for adjusting output signal
Value;The output end of the input contiguous function signal generating module of on-off circuit, the output end connection amplifier of on-off circuit
Input;The output end connection microelectrode exit of amplifier.Because microelectrode have it is multiple, amplifier also have it is multiple, often
The individual signal from on-off circuit output is accessed corresponding microelectrode after amplifier amplifies.Amplifier amplifies for adjustable gain
Device, gain that can be as needed to each amplifier are adjusted, so as to realize the signal width being applied on each microelectrode
Value is different.
Voltage stabilizing circuit module can be used the Switching Power Supply voltage stabilizing chip such as LM2575, MAX1715, efficiency high, small power consumption, specifically
Chip can be chip used according to PCB control circuits and digital display module supply voltage determine;Module, which occurs, for function signal to make
Chip MAX038 occurs with single chip integrated function to complete, high-frequency high-precision output waveform can be produced, output waveform distortion is small, drift
Small, wide frequency range is moved, the waveforms such as sine wave, square wave can be produced, and frequency, dutycycle are adjustable;Make in PCB control circuits
Realize that electric signal single channel inputs multiple-channel output with on-off circuit, adjustable gain function is realized using gain adjustable amplifier.
In the present embodiment, there are three groups of interdigital electrodes and one group of parallel pole, therefore PCB control circuits are that single channel inputs the output of four tunnels, are increased
Benefit is respectively set to 0 times, 1 times, 2 times and 3 times.
The break-make of microelectrode power on signal is by the chip in PCB control circuit modules and switch co- controlling, chip
The input of single channel electric signal, the output of multi-channel electric signal are realized, the species sum of electrode is accessed by PCB control circuits switch-mode regulation
Amount, the gating of multi-electrode is realized, change the time that cell is exposed in electric field, so as to control the work bar of cell electroporation
Part.
Interdigital electric field of the interdigital electrode 101 is more than the electric field between electrodes of parallel pole 102, when cell passes through upstream
When, electroporation can be implemented to cell;Cell after electroporation continues downward downstream, the electric field between electrodes of parallel pole 102
It is relatively low, cell electroporation state can be maintained, strengthens and improves the transfection efficiency of cell.
The making of microelectrode fluidic chip and be mounted to adjustable parameter single cell electroporation device process it is as follows:
(1) gold microelectrode is made using lift-off techniques on 4 cun of transparency carriers;Utilize and be based in 4 cun of silicon wafers
The soft light carving technology of SU-8 photoresists makes PDMS microfluidic channel layers;Glass, (poly-methyl methacrylate can be used in transparency carrier
Fat) transparent insulation material such as PMMA makes;The precious metal materials such as gold, platinum can be used by the technique such as electroplating or depositing in microelectrode
Make.Transparency carrier material ensure that can carry out optical microphotograph observation in experimentation;The materials chemistry such as gold, platinum strong inert,
Good conductivity, no biotoxicity.
(2) as shown in Figure 1 to 4, under the observation of stereomicroscope, punched on PDMS microfluidic channel layers,
To process cell suspending liquid entrance, sheath inflow entrance, cell outlet.
(3) transparency carrier is cleaned, dried up respectively with PDMS microfluidic channels layer, is subsequently placed in oxygen plasma cleaning
Permanent bonding is realized after carrying out surface modification treatment in machine.By on PDMS microfluidic channel layers under stereoscopic sem observation during bonding
Bonding four square alignment marks and being bonded with cross alignment mark center superposition on transparency carrier, to ensure that electrode is proper
It can be located in micro-fluidic electroporation passage.Then as shown in figure 4, inserting cell suspending liquid input on microelectrode fluidic chip
Pipe, sheath stream input pipe, cell efferent duct.
(4) as shown in figure 5, microelectrode fluidic chip is fixed in the chip slot in chip carrier, by PCB control circuits
Module and chip carrier fastened by screw, the spring probe and microelectrode for making control circuit realize electrical contact under stress, then
Connect power module of voltage regulation and module occurs for function signal, form the unicellular electricity of the adjustable parameter based on microelectrode fluidic chip and wear
Aperture apparatus.
Claims (10)
1. a kind of microelectrode fluidic chip, it is characterised in that including transparency carrier (10) and microfluidic channel layer (20), microfluid
Channel layer (20) is located above transparency carrier (10), and the microfluidic channel layer (20) includes sheath inflow entrance (201), cell suspends
Liquid entrance (202), sheath circulation road (203), cell suspending liquid passage (204), micro-fluidic electroporation passage (205) and cell outlet
(206);Sheath inflow entrance (201) connects with sheath circulation road (203), cell suspending liquid entrance (202) and cell suspending liquid passage
(204) connect, the end of sheath circulation road (203) is converged with cell suspending liquid passage (204) end and led to micro-fluidic electroporation
One end connection in road (205), the other end of micro-fluidic electroporation passage (205) connect with cell outlet (206);The transparent base
Microelectrode is integrated with plate (10), microelectrode includes interdigital electrode (101) and parallel pole (102), interdigital electrode (101) peace
Row electrode (102) is distributed in the lower section of micro-fluidic electroporation passage (205), and interdigital electrode (101) and parallel pole (102) from
Cell suspending liquid entrance (202) is sequentially distributed to cell outlet (206);Microelectrode includes microelectrode exit (103), microelectrode
Exit (103) is distributed in transparency carrier (10) both sides.
2. microelectrode fluidic chip according to claim 1, it is characterised in that the interdigital electrode (101) and/or parallel
Electrode (102) has multiple and uniform intervals to be distributed.
3. microelectrode fluidic chip according to claim 1, it is characterised in that the sheath circulation road (203) includes two
Passage tributary, two passage tributaries respectively from cell suspending liquid entrance (202) both sides around cell suspending liquid entrance (202) with it is thin
Born of the same parents' suspension passage (204) connects.
4. microelectrode fluidic chip according to claim 1, it is characterised in that also including sheath stream input pipe (301), cell
Suspension input pipe (302), cell efferent duct (303), cell suspending liquid input pipe (302) and cell suspending liquid entrance (202)
Connection, sheath stream input pipe (301) connect with sheath inflow entrance (201), and cell efferent duct (303) connects with cell delivery outlet (206).
5. microelectrode fluidic chip according to claim 1, it is characterised in that transparency carrier (10) is provided with bonding with ten
Word alignment mark (104), microfluidic channel layer (20) are provided with the bonding being engaged with bonding with cross alignment mark (104) and used
Four square alignment marks (207).
6. microelectrode fluidic chip according to claim 1, it is characterised in that the finger spacing of interdigital electrode (101) is 50
~80 μm;The electrode spacing of parallel pole (102) is 150~200 μm.
7. a kind of adjustable parameter single cell electroporation device using the microelectrode fluidic chip as described in claim 1-5, it is special
Sign is, including module, PCB control circuits and digital display module, chip carrier and micro- electricity occur for power module of voltage regulation, function signal
Pole fluidic chip, chip carrier are provided with chip slot, and the microelectrode fluidic chip is fixed in chip slot, PCB control circuits
And digital display module output end is spring probe, the spring probe makes electrical contact with microelectrode exit (103);Power module of voltage regulation
For module, PCB control circuits and digital display module and the power supply of microelectrode fluidic chip to occur for function signal;Function signal occurs
Module is used to export power supply perforation desired signal.
8. adjustable parameter single cell electroporation device according to claim 6, it is characterised in that the PCB control circuits
And digital display module includes on-off circuit and amplifier, single channel input is divided into multiple-channel output by on-off circuit, and amplifier is used to adjust
The amplitude of output signal;The output end of the input contiguous function signal generating module of on-off circuit, the output end of on-off circuit
Connect the input of amplifier;The output end connection microelectrode exit of amplifier.
9. adjustable parameter single cell electroporation device according to claim 7, it is characterised in that amplifier is adjustable gain
Amplifier.
10. adjustable parameter single cell electroporation device according to claim 7, it is characterised in that the interdigital electrode
(101) interdigital electric field is more than the electric field between electrodes of parallel pole (102).
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