CN107691922A - A kind of functional drink - Google Patents
A kind of functional drink Download PDFInfo
- Publication number
- CN107691922A CN107691922A CN201711023082.1A CN201711023082A CN107691922A CN 107691922 A CN107691922 A CN 107691922A CN 201711023082 A CN201711023082 A CN 201711023082A CN 107691922 A CN107691922 A CN 107691922A
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- leaf
- enzymolysis
- moringa
- extract
- raw material
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- 238000000703 high-speed centrifugation Methods 0.000 description 1
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- 229960000890 hydrocortisone Drugs 0.000 description 1
- 238000005984 hydrogenation reaction Methods 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
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- 238000001727 in vivo Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 230000008944 intestinal immunity Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
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- 239000008188 pellet Substances 0.000 description 1
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- 230000035699 permeability Effects 0.000 description 1
- SBNFWQZLDJGRLK-UHFFFAOYSA-N phenothrin Chemical compound CC1(C)C(C=C(C)C)C1C(=O)OCC1=CC=CC(OC=2C=CC=CC=2)=C1 SBNFWQZLDJGRLK-UHFFFAOYSA-N 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Chemical class 0.000 description 1
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- 229960002477 riboflavin Drugs 0.000 description 1
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- 150000007949 saponins Chemical class 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
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- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
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- 235000019155 vitamin A Nutrition 0.000 description 1
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- 235000010374 vitamin B1 Nutrition 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/72—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
- A23L2/74—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration using membranes, e.g. osmosis, ultrafiltration
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Microbiology (AREA)
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- Medicines Containing Plant Substances (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The present invention provides a kind of functional drink, includes the raw material of following composition by weight：Passion flower P.E 67 83, leaf of Moringa enzymolysis product 52 69, tea tree purple bud anthocyanidin 10 17, sodium carboxymethylcellulose 36, xylitol 9 21, citric acid 59, malic acid 13 18, Phyllanthus embical fruit extract 27 39, plant thalline crude extract 8 19, water 115 132.The present invention is mutually mixed using different types of plant component, is learnt from other's strong points to offset one's weaknesses, be can be made into mixed plant type beverage best in quality, taste is better.Solve the problems, such as that the fruit juice of single taste in the market has been difficult to meet the needs of people, can both meet that people for the pursuit in mouthfeel, also can further meet the pursuit on health diet, be advantageous to alleviate the inferior health problem of modern white collar.
Description
Technical field
The present invention relates to food technology, health management arts, and in particular to a kind of functional drink.
Background technology
Province of 31, China and municipality directly under the Central Government, 589 cities are related to according to portion, are collected into 51.3 ten thousand parts in all parts of the country effectively tune
The result for interrogating volume shows that the white collar inferior health ratio in main flow city, in overwork state close to sixty percent, is really anticipated up to 76%
" Healthy People " ratio in justice is extremely low（Less than 3%）, in the booming income crowd of 35-50 year, " biological age " overage trend substantially adds
It hurry up, average more than " calendar age " 10 years or so.
Wherein, Chinese civil servant is overweight or fat ratio is up to 40.7%, behind be dyslipidemia (28.7%) successively, blood
Reduce off-flavor is normal (14.2%), besides electrocardiographic abnormality（10.4%）.Civil servant's fatty liver and the three high relatively common mental labor of problem
Dynamic person is higher by 4.4% and 3.2%；Shoulder neck acid is swollen (75%) and eyes acid swollen (60%) is the most common problem of civil servant.It is former to study carefully it
Because being 58% civil servant " daily sitting handle official business 5-8 hours " with being shown in survey, more having 25% people, " daily sitting is done
Public 8-12 hours ", and civil servant bend over one's desk working, lack that physical training is relevant for a long time, do not cause oneself attention to health
And another aspect reason.
Therefore, " inferior health " problem is just constantly invading and harassing the young and middle-aged generation in China, and medicine can not only be leaned on by breaking away from sub-health state
Thing and tonic, to attempt to resist inferior health with healthy lifestyles.For example rational work rhythm, appropriate motion, have
The life style of rule, sufficient sleep etc..Adhere to for a long time " tonic adds motion ", therapeutic effect can build up one's health by taking tonic substantially than simple.
The content of the invention
In view of this, it can solve the problem that inferior health brings problem function type beverage it is an object of the invention to provide one kind, adopt
By the use of pure natural plant ingredients as main function formula, compounded by the synergistic between each functional component, there is provided a kind of long-term
Take can effectively strengthen immunity and the in vivo functional drink of antibacterial anti-inflammatory ability, provided to solve inferior health problem
A kind of new selection of unique flavor, color and luster and composite plant type drink good in taste and with better nutritivity value.
The technical scheme is that：A kind of functional drink, it is characterised in that include the raw material of following composition by weight：
Passion flower P.E 67-83, leaf of Moringa enzymolysis product 52-69, tea tree purple bud anthocyanidin 10-17, sodium carboxymethylcellulose 3-6,
Xylitol 9-21, citric acid 5-9, malic acid 13-18, Phyllanthus embical fruit extract 27-39, plant thalline crude extract 8-19, water 115-
132。
Further, the functional drink includes the raw material of following composition by weight：Passion flower P.E 72-78, Moringa
Leaf enzymolysis product 57-63, tea tree purple bud anthocyanidin 12-15, sodium carboxymethylcellulose 4-5, xylitol 11-17, citric acid 6-7,
Malic acid 15-17, Phyllanthus embical fruit extract 29-34, plant thalline crude extract 10-13, water 120-128.
Further, the functional drink includes the raw material of following composition by weight：Passion flower P.E 75, leaf of Moringa enzyme
Solution product 59, tea tree purple bud anthocyanidin 14, sodium carboxymethylcellulose 4, xylitol 13, citric acid 6, malic acid 16, Phyllanthus embical fruit carry
Take thing 31, plant thalline crude extract 12, water 125.
Further, by fresh passion flower-fruit peel wash clean, dry, crush；In material-water ratio m:V=1:15, by the matter of raw material
The pectase than adding 0.4%-0.8% is measured, 22-38min is digested at 45 DEG C；Destroy the enzyme treatment is carried out, filtering, leaves and takes filtrate.This
In invention, passionflower (Passiflora edulis) is Passifloraceae Passiflora plant, originates in Brazil, alive later
There is plantation boundary various regions.Passionflower containing more than 135 kinds of aromatic compound because being otherwise known as passion fruit.Passionfruit nutrition
Composition enriches, and the content of glutamic acid highest containing 7 kinds of organic acids, 17 kinds of amino acid and 21 kinds of trace elements, wherein passionflower, is
64. 90 mg.It is a kind of traditional containing abundant carbohydrate, polysaccharide, flavones and polyphenols in passion flower-fruit peel
Medicinal fruit.It has abundant medicines and health protection function, such as promoting blood circulation, enriching yin and nourishing kidney, promotes the production of body fluid to quench thirst, also with anti-Jiao
The therapeutic actions such as worry, calmness, anti-inflammatory, anti-habituation, in addition, also having the physiological functions such as treatment epilepsy, hypoglycemic, reducing blood lipid.
Further, the preparation method of the leaf of Moringa enzymolysis product is：Fresh leaf of Moringa is cleaned, by leaf of Moringa and pure
Water purification is according to m:V=1:5 ratio mixing, is blended using mixer, and 1.7%-2.5% pectin is added by the original quality of raw material
Enzyme, reach 3.5 using lemon acid for adjusting pH, 8-12min is digested at 50 DEG C, preliminary enzymolysis liquid is obtained after enzymolysis, will tentatively digest
Liquid boils 10min enzyme deactivations, is cooled to 50-55 DEG C, the pH of preliminary enzymolysis liquid is adjusted into 6.5 using sodium tripolyphosphate, by raw material
Original quality adds 0.5%-0.9% compound fertilizer production, digests 0.8-1.5h, and enzymolysis boils 20min enzyme deactivations after terminating, and uses
The nylon cloth filtering of 200 mesh, then filtrate is filtered by molecular cut off for 5kDa device for ultrafiltration membrane, obtain the leaf of Moringa of clarification
Enzymolysis product.In the present invention, the Moringa (Moringa) originates in India, and Yin Qiye, flower, bark, root, seed, branch and stem are equal
With higher effective component and nutritive value, it is described as " tree of miracle " by western scientist.Wherein per in 100g leaf of Moringa powder
Mineral matter, vitamin and the essential amino acid content contained is higher than the daily ingestion of standard of world health organisation recommendations.Grind
Study carefully discovery, leaf of Moringa has hypoglycemic, lowering blood pressure and blood fat, reduces the work(such as cholesterol, anti-oxidant, anti-aging, strengthen immunity
Effect acts on.The function ingredients extracted by the preparation method of the present invention from leaf of Moringa, strengthen immunity, body for the present invention
Interior antibacterial anti-inflammatory ability has good facilitation.
Further, the preparation method of the Phyllanthus embical fruit extract is：By Phyllanthus embical fruit and pure water according to m:V=1:20
Ratio is mixed, and after boiling 30 min, the Phyllanthus embical fruit and aqueous mixtures that will be cooled to room temperature are added in colloid mill, and regulation gap is
0.1 mm, grind three times, by the original quality of raw material than adding 0.5%-1.2% pectases, reached using lemon acid for adjusting pH
3.5, digest 15-35min at 50 DEG C, after enzymolysis, enzymolysis liquid is boiled into 30 min enzyme deactivations, is cooled to room temperature, with the Buddhist nun of 200 mesh
Imperial cloth filtering, then filtrate is filtered by molecular cut off for 2 kDa device for ultrafiltration membrane, obtain the Phyllanthus embical fruit extract solution of clarification.
Phyllanthus embical fruit also known as emblic, it is the sweet perennial deciduous tree of category of Euphorbiaceae oil in the present invention.Yunnan Province is national wild
Raw Phyllanthus embical fruit is distributed most wide, yield highest province.Count according to investigations, Yunnan Province ten thousand mu of Phyllanthus embical fruit Linda 60-70 in blocks at present,
Suitable dough product produces nearly ten thousand tons of fresh fruit per year up to more than 3,000,000 mu.So huge resource is not utilized largely at present, is not had more
Have carry out profound comprehensive process-.In recent years, China's medical field and scientific research department were by repetition test and constantly research, card
Bright Phyllanthus embical fruit has obvious anti-aging effects；Have to synthesis of the strong carcinogen N- nitroso compounds in animal and human body bright
Aobvious blocking effect, blocking rate is up to more than 90.11%.In addition, Phyllanthus embical fruit contains substantial amounts of Vc, carbohydrate, organic acid, list
Rather, also containing protein, fat, vitamin B1, vitamin B2, vitamin A, carrotene, cellulose, pectin, alkaloid and
Calcium, phosphorus, iron, potassium, sodium etc., there is clearing heat and detoxicating, relieving sore-throat resolving sputum, promote the production of body fluid to quench thirst, solution is tired of the effect of sobering up, and it is treated to abscess of throat
Imitate especially pronounced.
Further, the preparation method of plant thalline crude extract is：The stem of fresh luffa vine and leaf is taken to clean to not bright
Aobvious impurity, after surface sterilization processing, it is seeded to PDA（Separate endogenetic fungus）With Gause I culture medium（Raw unwrapping wire in separation
Bacterium）Upper culture 2-5d, then separation obtain bacterium colony；Picking inoculated by hypha block is in the cone equipped with fermentation medium from activated inclined plane
In shape bottle, 37 DEG C are put, 100r/min shaking table shaken cultivations 5d；Fermentation culture medium removes thalline, and filtrate is with isometric ethyl acetate
Extraction 3 times, merge organic phase, 50 DEG C are concentrated under reduced pressure and are evaporated, as mixed bacteria liquid crude extract；The thalline for centrifuging and being filtrated to get,
With appropriate 95% edible ethanol condensing reflux 3 times, merge ethanol phase, be concentrated under reduced pressure and be evaporated, as plant thalline crude extract.Plant
Endophyte is a huge microbe groups, and its species is various, is distributed in the different parts of different host plants, therefore can produce
Raw a variety of different metabolites, have antitumor, antibacterial, antiviral, desinsection, immunosupress, anti-oxidant and hypoglycemic isoreactivity.
Simultaneously as plant itself act as natural selection system to toxicity molecule, the active material poison in endophyte of plant source is caused
Property is relatively low.The present invention by experimental results demonstrate, by the present invention method prepare plant thalline crude extract, use
It is the endophyte group of luffa vine, and sponge gourd belongs to dicotyledon medicine cucurbitaceous plant, with ripening fruits, fruit network, leaf, rattan, root
And seed is used as medicine.The medical value of sponge gourd is very high, and whole body can all enter.All kinds of nutrition are higher in melon food contained by sponge gourd, institute
Have necessarily containing particular matters such as saponins, luffein, lymphatic temperament, wood glue, citrulling, xylan and interferon
Special role.At present, the sponge gourd for vegetables mainly has two kinds, i.e. luffa-smooth loofah and angular sponge gourd.The former on both sides of the Changjiang River has
Cultivation, the latter mainly cultivate in south China, the tasty succulence of fruit, can fry also can cooking, bias is cold.It our experiments show that, luffa vine powder
Decoction has weaker inhibitory action to respiratory tract common bacteria with alcohol preserved material, and slightly strong, the fresh juice of luffa vine is acted on to pneumococcus
Without bacteriostasis.Plant thalline crude extract prepared by the present invention has excellent antibacterial activity for animal body, while does not almost have
There is bio-toxicity, bioactivity can either be played in the case of low dosage, be adapted to edible for a long time.
In the present invention, the tea tree purple bud anthocyanidin, used tea tree purple bud is as specific Resources of Tea Plant, in place
Occupy very big ratio in tea tree sexual varieties.Representative of China's property kind mainly has Tea Inst., Yun-nan Academy of Agricultural Science's seed selection
" tongue is fragrant purple " of " purple beautiful ", Zhejiang Province's seed selection, " the red bud Buddha's hand " in Fujian etc..In spring ordinary group kind, 30% or so
Reddish violet is presented in bud-leaf, then there is the different degrees of reddish violet of 88.7% presentation in summer, and the heliotrope new shoot on tea tree is compared with pure green
Bud-leaf contains more Tea Polyphenols, catechin and flavone compound, and wherein anthocyanidin content height is it in the main of reddish violet
Reason.It has been investigated that tea tree purple bud anthocyanidin as a kind of strong free radical scavenger, have anti-oxidant, hypoglycemic, anti-inflammatory,
The plurality of health care functions such as decompression.In the present invention, the tea tree purple bud anthocyanidin assigns beverage of the present invention good color and luster.Especially
, the tea tree purple bud anthocyanidin can be realized by this area any prior art.
In the present invention, in food storage or process, due to the result of bacterial action, from protein or Amino acid score
Solution, mostly in such as amine, ammonia, the material of nitrogen-containing heterocycle compound bad flavor of alkalescence, using citric acid and malic acid
Distribution, the effect for neutralizing and de-tasting can be reached.In the present invention, stabilizer can be used as to make the present invention can using sodium carboxymethylcellulose
Obtain comparatively ideal sensory effects.Particularly, the function ingredients in the present invention have natural anti-oxidation function, are not required to additionally add
Add antioxidant, there can be natural antiseptic property, there is long quality guarantee period.
The preparation method of drinks of the present invention is：S1. Passion flower P.E, leaf of Moringa enzymolysis product, Phyllanthus embical fruit are prepared
Extract, plant thalline crude extract isoreactivity composition；S2. by each component mixing preparation in proportion, the mixed liquor high speed centrifugation
Separation, obtains standby separating liquid；S3. by separating liquid homogenizer homogeneous, homogenizing fluid, homogenization pressure 25MPa are obtained；Stream
Speed is 10dm3/h；S4. the beverage after homogeneous is deaerated using water circulating pump, under 0.09 Mpa vacuums, deaerated 5 minutes；
S5. filtration sterilization is carried out to the beverage after degassing using miillpore filter；S6. by filtered beverage filling into vial, so
Rear seal-cover.
Particularly, the reagent employed in the present invention is food-grade.
The present invention passes through Passion flower P.E, leaf of Moringa enzymolysis product, tea tree purple bud anthocyanidin, Phyllanthus embical fruit extract, plant
Synergistic compounding between thing thalline crude extract component, the anti-inflammatory for reaching good is antibacterial and immunoregulation effect, its anti-inflammatory are antibacterial
Effect is mainly reached by one of approach of following several respects：Suppress migration of the leucocyte to inflammation part；Activated macrophage
Nitric oxide is produced, nitric oxide is the medium molecule of immune response and inflammatory reaction, participates in body inflammatory reaction and immune tune
Section, the physiology and pathologic process of the multiple systems of body are participated in, suppress the growth of pathogenic microorganism or kill intracellular pathogen；Suppression
The permeability of capillary processed, reduce diffusate volume；IL-6 generation can be suppressed, and lower NF- κ B nuclear translocation, suppressed
Th2 produces the cell factor such as IL-4, IL-5, IL-13, suppresses the release of Pro-inflammatory mediator, adjust some inflammation correlations because
Son generation and play antiinflammatory action；Strengthen the phagocytic activity of macrophage.
In the present invention, coordinating the active thalline crude extract extracted out of plant, active drive member enters in enteron aisle,
On the one hand it can be colonized in enteron aisle, maintain the balance of intestinal microflora；On the other hand it is active drive member and west kind
Lotus extract, leaf of Moringa enzymolysis product, tea tree purple bud anthocyanidin, Phyllanthus embical fruit extract collective effect in the immune system of host,
Intestine immunity is induced, and stimulates thymus gland, the immune organ such as spleen, promotes macrophage activity, by strengthening B, T lymphocyte pair
The reactivity of antigenic stimulus, specificity immuning activity is played, so as to strengthen the immunologic function of body, is easily absorbed by the body, bar
The crowd's long-term use of part 95% is without malaise symptoms.
The beneficial effects of the present invention are：
The present invention is mutually mixed using different types of plant component, is learnt from other's strong points to offset one's weaknesses, and it is mixed to can be made into that best in quality, taste is better
Close plant type beverage.Solve the problems, such as that the fruit juice of single taste in the market has been difficult to meet the needs of people, both can be with
Meet that people for the pursuit in mouthfeel, also can further meet the pursuit on health diet, be advantageous to alleviate modern white collar
Inferior health problem.
Embodiment
Technical scheme is clearly and completely described below in conjunction with embodiment, it is clear that described reality
It is only part of the embodiment of the present invention to apply example, rather than whole embodiments.
Embodiment 1
A kind of functional drink, include the raw material of following composition by weight：Passion flower P.E 75, leaf of Moringa enzymolysis product 59, tea
Set purple bud anthocyanidin 14, sodium carboxymethylcellulose 4, xylitol 13, citric acid 6, malic acid 16, Phyllanthus embical fruit extract 31, plant
Thalline crude extract 12, water 125.
By fresh passion flower-fruit peel wash clean, dry, crush；In material-water ratio m:V=1:15, added by the mass ratio of raw material
0.55% pectase, 29min is digested at 45 DEG C；Destroy the enzyme treatment is carried out, filtering, leaves and takes filtrate.
The preparation method of the leaf of Moringa enzymolysis product is：Fresh leaf of Moringa is cleaned, by leaf of Moringa and pure water according to
m:V=1:5 ratio mixing, is blended using mixer, 2.1% pectase is added by the original quality of raw material, using citric acid
Regulation pH reaches 3.5, and 9min is digested at 50 DEG C, preliminary enzymolysis liquid is obtained after enzymolysis, preliminary enzymolysis liquid is boiled into 10min enzyme deactivations,
52 DEG C are cooled to, the pH of preliminary enzymolysis liquid is adjusted to 6.5 using sodium tripolyphosphate, adding 0.7% by the original quality of raw material answers
Flavor protease is closed, digests 1.1h, enzymolysis boils 20min enzyme deactivations after terminating, and is filtered with the nylon cloth of 200 mesh, then filtrate is led to
Cross molecular cut off to filter for 5kDa device for ultrafiltration membrane, obtain the leaf of Moringa enzymolysis product of clarification.
The preparation method of the Phyllanthus embical fruit extract is：By Phyllanthus embical fruit and pure water according to m:V=1:20 ratio mixing,
After boiling 30 min, the Phyllanthus embical fruit and aqueous mixtures that will be cooled to room temperature are added in colloid mill, and regulation gap is 0.1 mm, grinding
Three times, by the original quality of raw material than adding 0.9% pectase, reach 3.5 using lemon acid for adjusting pH, 15- is digested at 50 DEG C
35min, after enzymolysis, enzymolysis liquid is boiled into 30 min enzyme deactivations, is cooled to room temperature, filtered with the nylon cloth of 200 mesh, then filtrate is led to
Cross molecular cut off to filter for 2 kDa device for ultrafiltration membrane, obtain the Phyllanthus embical fruit extract solution of clarification.
The preparation method of the plant thalline crude extract is：The stem of fresh luffa vine and leaf is taken to clean to not obvious miscellaneous
Matter, after surface sterilization processing, it is seeded on PDA and Gause I culture medium and cultivates 4d, then separation obtains bacterium colony；It is oblique from activation
Picking inoculated by hypha block puts 37 DEG C, 100r/min shaking table shaken cultivations 5d in the conical flask equipped with fermentation medium in face；Hair
Ferment culture removes thalline, and filtrate is extracted 3 times with isometric ethyl acetate, merges organic phase, and 50 DEG C are concentrated under reduced pressure and are evaporated, as
Mixed bacteria liquid crude extract；The thalline for centrifuging and being filtrated to get, with appropriate 95% edible ethanol condensing reflux 3 times, merge ethanol phase,
It is concentrated under reduced pressure and is evaporated, as plant thalline crude extract.Particularly, the mixing total plate count of the plant thalline crude extract is 3.1*
106cfu/g。
Embodiment 2
A kind of functional drink, include the raw material of following composition by weight：Passion flower P.E 72, leaf of Moringa enzymolysis product 57, tea
Set purple bud anthocyanidin 12, sodium carboxymethylcellulose 4, xylitol 11, citric acid 6, malic acid 15, Phyllanthus embical fruit extract 29, plant
Thalline crude extract 10, water 120.
By fresh passion flower-fruit peel wash clean, dry, crush；In material-water ratio m:V=1:15, added by the mass ratio of raw material
0.4% pectase, 38min is digested at 45 DEG C；Destroy the enzyme treatment is carried out, filtering, leaves and takes filtrate.
The preparation method of the leaf of Moringa enzymolysis product is：Fresh leaf of Moringa is cleaned, by leaf of Moringa and pure water according to
m:V=1:5 ratio mixing, is blended using mixer, 1.7% pectase is added by the original quality of raw material, using citric acid
Regulation pH reaches 3.5, and 12min is digested at 50 DEG C, preliminary enzymolysis liquid is obtained after enzymolysis, preliminary enzymolysis liquid is boiled into 10min enzyme deactivations,
50 DEG C are cooled to, the pH of preliminary enzymolysis liquid is adjusted to 6.5 using sodium tripolyphosphate, adding 0.5% by the original quality of raw material answers
Flavor protease is closed, digests 1.5h, enzymolysis boils 20min enzyme deactivations after terminating, and is filtered with the nylon cloth of 200 mesh, then filtrate is led to
Cross molecular cut off to filter for 5kDa device for ultrafiltration membrane, obtain the leaf of Moringa enzymolysis product of clarification.
The preparation method of the Phyllanthus embical fruit extract is：By Phyllanthus embical fruit and pure water according to m:V=1:20 ratio mixing,
After boiling 30 min, the Phyllanthus embical fruit and aqueous mixtures that will be cooled to room temperature are added in colloid mill, and regulation gap is 0.1 mm, grinding
Three times, by the original quality of raw material than adding 0.5% pectase, reach 3.5 using lemon acid for adjusting pH, digested at 50 DEG C
35min, after enzymolysis, enzymolysis liquid is boiled into 30 min enzyme deactivations, is cooled to room temperature, filtered with the nylon cloth of 200 mesh, then filtrate is led to
Cross molecular cut off to filter for 2 kDa device for ultrafiltration membrane, obtain the Phyllanthus embical fruit extract solution of clarification.
The preparation method of the plant thalline crude extract is：The stem of fresh luffa vine and leaf is taken to clean to not obvious miscellaneous
Matter, after surface sterilization processing, it is seeded on PDA and Gause I culture medium and cultivates 2d, then separation obtains bacterium colony；It is oblique from activation
Picking inoculated by hypha block puts 37 DEG C, 100r/min shaking table shaken cultivations 5d in the conical flask equipped with fermentation medium in face；Hair
Ferment culture removes thalline, and filtrate is extracted 3 times with isometric ethyl acetate, merges organic phase, and 50 DEG C are concentrated under reduced pressure and are evaporated, as
Mixed bacteria liquid crude extract；The thalline for centrifuging and being filtrated to get, with appropriate 95% edible ethanol condensing reflux 3 times, merge ethanol phase,
It is concentrated under reduced pressure and is evaporated, as plant thalline crude extract.Particularly, the mixing total plate count of the plant thalline crude extract is 6.5*
105cfu/g。
Embodiment 3
A kind of functional drink, include the raw material of following composition by weight：Passion flower P.E 78, leaf of Moringa enzymolysis product 63, tea
Set purple bud anthocyanidin 15, sodium carboxymethylcellulose 5, xylitol 17, citric acid 7, malic acid 17, Phyllanthus embical fruit extract 34, plant
Thalline crude extract 13, water 128.
By fresh passion flower-fruit peel wash clean, dry, crush；In material-water ratio m:V=1:15, added by the mass ratio of raw material
0.8% pectase, 22min is digested at 45 DEG C；Destroy the enzyme treatment is carried out, filtering, leaves and takes filtrate.
The preparation method of the leaf of Moringa enzymolysis product is：Fresh leaf of Moringa is cleaned, by leaf of Moringa and pure water according to
m:V=1:5 ratio mixing, is blended using mixer, 2.5% pectase is added by the original quality of raw material, using citric acid
Regulation pH reaches 3.5, and 8min is digested at 50 DEG C, preliminary enzymolysis liquid is obtained after enzymolysis, preliminary enzymolysis liquid is boiled into 10min enzyme deactivations,
55 DEG C are cooled to, the pH of preliminary enzymolysis liquid is adjusted to 6.5 using sodium tripolyphosphate, adding 0.9% by the original quality of raw material answers
Flavor protease is closed, digests 0.8h, enzymolysis boils 20min enzyme deactivations after terminating, and is filtered with the nylon cloth of 200 mesh, then filtrate is led to
Cross molecular cut off to filter for 5kDa device for ultrafiltration membrane, obtain the leaf of Moringa enzymolysis product of clarification.
The preparation method of the Phyllanthus embical fruit extract is：By Phyllanthus embical fruit and pure water according to m:V=1:20 ratio mixing,
After boiling 30 min, the Phyllanthus embical fruit and aqueous mixtures that will be cooled to room temperature are added in colloid mill, and regulation gap is 0.1 mm, grinding
Three times, by the original quality of raw material than adding 1.2% pectase, reach 3.5 using lemon acid for adjusting pH, digested at 50 DEG C
15min, after enzymolysis, enzymolysis liquid is boiled into 30 min enzyme deactivations, is cooled to room temperature, filtered with the nylon cloth of 200 mesh, then filtrate is led to
Cross molecular cut off to filter for 2 kDa device for ultrafiltration membrane, obtain the Phyllanthus embical fruit extract solution of clarification.
The preparation method of the plant thalline crude extract is：The stem of fresh luffa vine and leaf is taken to clean to not obvious miscellaneous
Matter, after surface sterilization processing, it is seeded on PDA and Gause I culture medium and cultivates 5d, then separation obtains bacterium colony；It is oblique from activation
Picking inoculated by hypha block puts 37 DEG C, 100r/min shaking table shaken cultivations 5d in the conical flask equipped with fermentation medium in face；Hair
Ferment culture removes thalline, and filtrate is extracted 3 times with isometric ethyl acetate, merges organic phase, and 50 DEG C are concentrated under reduced pressure and are evaporated, as
Mixed bacteria liquid crude extract；The thalline for centrifuging and being filtrated to get, with appropriate 95% edible ethanol condensing reflux 3 times, merge ethanol phase,
It is concentrated under reduced pressure and is evaporated, as plant thalline crude extract.Particularly, the mixing total plate count of the plant thalline crude extract is 2.2*
107cfu/g。
Embodiment 4
A kind of functional drink, include the raw material of following composition by weight：Passion flower P.E 67, leaf of Moringa enzymolysis product 52, tea
Set purple bud anthocyanidin 10, sodium carboxymethylcellulose 3, xylitol 9, citric acid 5, malic acid 13, Phyllanthus embical fruit extract 27, plant bacterium
Body crude extract 8, water 115.
Embodiment 5
A kind of functional drink, include the raw material of following composition by weight：Passion flower P.E 83, leaf of Moringa enzymolysis product 69, tea
Set purple bud anthocyanidin 17, sodium carboxymethylcellulose 6, xylitol 21, citric acid 9, malic acid 18, Phyllanthus embical fruit extract 39, plant
Thalline crude extract 19, water 132.
Comparative example 1
A kind of functional drink, include the raw material of following composition by weight：Leaf of Moringa enzymolysis product 59, tea tree purple bud anthocyanidin 14,
Sodium carboxymethylcellulose 4, xylitol 13, citric acid 6, malic acid 16, Phyllanthus embical fruit extract 31, plant thalline crude extract 12, water
125.Particularly, leaf of Moringa enzymolysis product described in the present embodiment, Phyllanthus embical fruit extract, plant thalline crude extract preparation method with
Embodiment 1 is consistent.
Comparative example 2
A kind of functional drink, include the raw material of following composition by weight：Passion flower P.E 75, tea tree purple bud anthocyanidin 14, carboxylic
Sodium carboxymethylcellulose pyce 4, xylitol 13, citric acid 6, malic acid 16, Phyllanthus embical fruit extract 31, plant thalline crude extract 12, water
125.Particularly, Passion flower P.E, Phyllanthus embical fruit extract, the preparation method and reality of plant thalline crude extract described in the present embodiment
It is consistent to apply example 1.
Comparative example 3
A kind of functional drink, include the raw material of following composition by weight：Passion flower P.E 75, leaf of Moringa enzymolysis product 59, tea
Set purple bud anthocyanidin 14, sodium carboxymethylcellulose 4, xylitol 13, citric acid 6, malic acid 16, plant thalline crude extract 12, water
125.Particularly, Passion flower P.E described in the present embodiment, leaf of Moringa enzymolysis product, plant thalline crude extract preparation method with
Embodiment 1 is consistent.
Comparative example 4
A kind of functional drink, include the raw material of following composition by weight：Passion flower P.E 75, leaf of Moringa enzymolysis product 59, carboxylic
Sodium carboxymethylcellulose pyce 4, xylitol 13, citric acid 6, malic acid 16, Phyllanthus embical fruit extract 31, plant thalline crude extract 12, water
125.Particularly, Passion flower P.E, leaf of Moringa enzymolysis product, Phyllanthus embical fruit extract, plant thalline slightly carry described in the present embodiment
The preparation method of thing is consistent with embodiment 1.
Comparative example 5
A kind of functional drink, include the raw material of following composition by weight：Passion flower P.E 75, leaf of Moringa enzymolysis product 59, tea
Set purple bud anthocyanidin 14, sodium carboxymethylcellulose 4, xylitol 13, citric acid 6, malic acid 16, Phyllanthus embical fruit extract 31, water
125.Particularly, the preparation method and reality of Passion flower P.E, leaf of Moringa enzymolysis product, Phyllanthus embical fruit extract described in the present embodiment
It is consistent to apply example 1.
Measure of merit
Method of testing in the present invention is reference《Health food is examined and assessment technique enforcement of regulations handbook》, according to the state of correlation
Family's standard formulation, in the present invention processing of all experimental datas statistical analysis, the functional survey of institute are carried out using the softwares of SPSS 16.0
The data for trying effect use（）Represent.
1. beverage sensory evaluation
Using sensory evaluation method, the color and luster, flavor, mouthfeel of water chestnut skin flavor beverage are scored for deliberated index, ask 8 tools
There is senior beverage to judge the expert of experience to taste, given a mark after control sensory judgments table identification, full marks 100 divide, and remove a highest
Point and one minimum point, then average as evaluation result.Standards of grading such as following table.
Tested by sensory evaluation, the sensory evaluation score such as following table of each embodiment and comparative example.
2. antiphlogistic effects are tested
Antiphlogistic effects test is carried out to embodiment 1-5 and comparative example 1-5, mouse 72 is screened, is bisected into 12 groups at random.Setting one
Group blank control group, gives 0.2mL/20g physiological saline；One positive drug control group, 1 property gives 0.2mL/20g hydrogenations can
Loose parenteral solution.Embodiment 1-5 and comparative example 1-5 is test group, dosage 0.2mL/20g, successive administration 10d.Last is given
Medicine 1h, mouse right ear is wide to apply caused by dimethylbenzene xylene inflammation.Mouse is put to death after 1h, cuts ears diameter 9mm card punch in same portion
Auricle is laid in position, weighs and records.The experimental results are shown inthe following table.
Note：" a " represents there is significant difference with blank group；" b " represents there is significant difference with positive group.
Antiphlogistic effects confirmatory experiment
2.1 mice caused by dimethylbenzene xylene auricle edemas are tested
Mouse 50, is randomly divided into 5 groups.A groups are blank control group, give 0.2mL/20g physiological saline, and B, C, D group are experiment
Group, embodiment 1 solution 0.1mL/20g, 0.2mL/20g and 0.4mL/20g, successive administration 10d are given respectively.E groups are positive drug
Thing control group, 0.2mL/20g hydrocortisone parenteral solutions are disposably given, it is as a result as shown in the table.
Note：" a " represents there is significant difference with blank group；" b " represents there is significant difference with positive group.
2.2 swollen hyperplasia of rat granuloma are tested
Rat 25 is taken, with 1% anaesthetized with pentobarbital, lower abdomen unhairing sterilization, by 50mg sterile cotton balls implantation left side groin
Subcutaneously, sew up a wound.It is postoperative to be randomly divided into 5 groups, every group 5, start to be administered after 1h, be grouped and be administered same 2.1, successive administration
10d.Take out granuloma induced by implantation of cotton pellets tissue within 11st day, 60 DEG C of drying, weigh and record in drying baker, as a result as shown in the table.
Note：" a " represents there is significant difference with blank group；" b " represents there is significant difference with positive group.
3. immunomodulatory effect is tested
To be dried by drinks thickening made from embodiment 1, be prepared into it is powdered, as test sample.It is positive right
According to product：Its vigorous and graceful board Soybean Peptide albumen powder；Source：Beijing Tongrentang Health Pharmaceutical Co., Ltd.；Adult is clinical to be recommended to use
Amount：0.167g.kg-1.d-1。
Animal subject strain and rank：BALB/c mouse, Hartley cavys, SPF levels；Quantity and sex：BALB/c is small
Mouse 60, male；Hartley cavys 6,.Buy body weight BALB/c mouse 17-19g, Hartley cavy 250-
350g.During experiment, mouse ad lib drinking-water, mouse state is observed daily.
Dose design is carried out according to the basic condition of sample, sample it is basic, normal, high by adult 5 times of quantity, 10 times,
20 times of design dosage, respectively 0.15g.kg-1.d-1、 0.30g.kg-1.d-1 、0.60g.kg-1.d-1；Positive group dosage is proportionately
5 times of designs of people's recommended dose, are 0.835g.kg-1.d-1.60 male mices are respectively divided into 5 groups at random（Blank control group,
The basic, normal, high dosage group of positive controls, given the test agent）, every group 12, wherein 2 are only used as standby animal.Each group mouse is daily
By 0.1mL/10g body weight gavage respective sample liquid, blank group gives equivalent pure water, 1 time a day, successive administration 30 days.Experiment
Beginning, off-test are weighed in 1 time, are weighed in weekly during experiment 1 time.
3.1 mouse lymphocyte transformation experiments
Last dose 1h, it is sterile to take spleen to be placed in the culture dish for filling RPMI-1640 nutrient solutions, and 200 eye mesh screens are placed, with note
Emitter piston is ground above it is made splenocyte suspension, packing splenocyte suspension to the centrifuge tube added with lymphocyte separation medium
In.400g, 20min is centrifuged at 20 DEG C, centrifugation finishes, and takes out centrifuge tube medium size lymphocyte layer centrifuge tube, adds RPMI-1640 trainings
Nutrient solution is washed for several times, 250g, and 10min is centrifuged at 4 DEG C.After washing, abandoning supernatant adds complete medium, and piping and druming is equal
It is even, expect that blue dyeing counting ensures more than 95% living cell rate with platform, cell concentration is adjusted to 2.5 × 105Individual/ml.
Holes is divided to add in 48 well culture plates every part of splenocyte suspension, per hole 0.5ml, a hole adds 25 ul ConA liquid
（Equivalent to 5ug/ml）, another hole is placed in 5%CO as control2,37℃ CO2Gently sucked per hole after 48h is cultivated in incubator
The ml of clear liquid 0.3,0.3 ml RPMI-1640 nutrient solutions are added, while add the ul/ holes of 8 reagents of CCK 50, continue to cultivate 2 h.
After terminating culture, piping and druming is uniform, is then dispensed into 96 well culture plates, makees 3 parallel laboratory tests per hole, and 450 nm are surveyed with ELIASA
OD value under wavelength.The multiplication capacity of lymphocyte is by adding the OD value in ConA holes and being not added with the optical density in ConA holes
The difference of value represents.The experimental results are shown inthe following table.
Note：" a " represents there is significant difference with blank group；" b " represents there is significant difference with positive group.
3.2 mouse humoral immune functions（Serum hemolysin）Influence
Administration 25 days, taken off the sheep blood 3 times of fiber with brine (2000r/min, 10min), in hematocrit SRBC plus
Enter physiological saline and be made into 2%（v/v）Cell suspension, every mouse peritoneal injection 0.2ml, at the 30th day, after 1h is administered, weigh,
All mouse extract eyeball and take blood 0.8ml to stand 1h in centrifuge tube, centrifuge 2000r/min, 10min, collect serum.Cervical vertebra takes off
Mortar puts to death mouse.300 times of serum is diluted with SA buffer solutions takes 1ml to put in test tube, adds 10% again（v/v）
SRBC0.5ml, with SA buffer solutions 9 times of complement 1ml is diluted, control tube is set（Serum is replaced with SA）, 37 DEG C of waters bath with thermostatic control
20min, then ice bath, it is then centrifuged for 2000r/min, 10min.Supernatant 1ml is taken in new test tube, add 3.75ml Dou Shi reagents,
0.25ml 10%（v/v）SRBC, fully mix, stand 10min, using control tube as blank, determine each Guan Guangmi at 540nm respectively
Angle value, the amount of hemolysin is with half hemolytic value（HC50）Represent.As a result it is as shown in the table.The experimental results are shown inthe following table.
Note：" a " represents there is significant difference with blank group；" b " represents there is significant difference with positive group.
The measure that 3.3 mouse monokaryons-macrophage phagocytic function and immune organ influence
Last dose 1h, by body weight from tail vein injection physiological saline 1:The india ink of 3 dilutions（10mL/kg）, treat prepared Chinese ink
Injection, immediately timing.2,10min after injection prepared Chinese ink, respectively from orbital venous plexus 20 μ L of blood sampling, and 2mL 0.1% is added to immediately
Na2CO3In solution, with Na2CO3Solution makees blank control, and OD value is determined at 600nm wavelength with spectrophotometer（OD）,
Calculate phagocytic index a.
Dislocate and put to death after mouse blood sampling, solution takes liver, spleen and thymus gland, accurate after filter paper blots internal organs peripheral blood
Weigh, calculate internal organs/body weight ratio.The experimental results are shown inthe following table.
Note：" a " represents there is significant difference with blank group；" b " represents there is significant difference with positive group.
3.4 NK cytoactive detections
Last dose 1h, it is sterile to take spleen, it is sterile to take spleen to be placed in the culture dish for filling RPMI-1640 nutrient solutions, and place 200 mesh
Screen cloth, ground above it with syringe piston and splenocyte suspension is made, packing splenocyte suspension is to added with separation of lymphocytes
In the centrifuge tube of liquid.400g, 20min is centrifuged at 20 DEG C, centrifugation finishes, and takes out centrifuge tube medium size lymphocyte layer centrifuge tube, adds
RPMI-1640 nutrient solutions are washed for several times, 250g, and 10min is centrifuged at 4 DEG C.After washing, abandoning supernatant, training completely is added
Base is supported, piping and druming is uniform, expects blue dyeing counting viable count with platform（Should be more than 95%）, finally with the complete culture solutions of RPMI 1640
It is 2 × 10 to adjust cell concentration7Individual/mL.
In proportion by effector cell and target cell（50:1）Each 100ul adds U-shaped 96 orifice plate；Target cell and nutrient solution are each
100ul adds target cell Spontaneous release hole；Target cell and each 100ul of 0.25%Triton add target cell maximum release aperture.Every group
It is all provided with 3 parallel holes, 37 DEG C, after 5%CO2 incubator cultures 4h, centrifuges 1500rpm, 5min.Per hole Aspirate supernatant 100ul in
Flat 96 orifice plate, LDH matrix liquid 100ul/ holes are added, react 3-10min, add 1mol/ml HCL 30ul/ holes, enzyme mark
490nm determines OD value.The experimental results are shown inthe following table.
Note：" a " represents there is significant difference with blank group；" b " represents there is significant difference with positive group.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, and do not carrying on the back
In the case of spirit or essential attributes from the present invention, the present invention can be realized in other specific forms.Therefore, no matter from which
From the point of view of a bit, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention will by appended right
Ask rather than described above limits, it is intended that all changes in the implication and scope of the equivalency of claim will be fallen
Include in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It is appreciated that other embodiment.It is noted that the technical characteristic not being described in detail in the present invention, can pass through this
Field any prior art is realized.
Claims (7)
1. a kind of functional drink, it is characterised in that include the raw material of following composition by weight：Passion flower P.E 67-83, Moringa
Leaf enzymolysis product 52-69, tea tree purple bud anthocyanidin 10-17, sodium carboxymethylcellulose 3-6, xylitol 9-21, citric acid 5-9, apple
Tartaric acid 13-18, Phyllanthus embical fruit extract 27-39, plant thalline crude extract 8-19, water 115-132.
2. functional drink according to claim 1, it is characterised in that include the raw material of following composition by weight：Passionflower
Extract 72-78, leaf of Moringa enzymolysis product 57-63, tea tree purple bud anthocyanidin 12-15, sodium carboxymethylcellulose 4-5, xylitol
11-17, citric acid 6-7, malic acid 15-17, Phyllanthus embical fruit extract 29-34, plant thalline crude extract 10-13, water 120-128.
3. functional drink according to claim 2, it is characterised in that include the raw material of following composition by weight：Passionflower
Extract 75, leaf of Moringa enzymolysis product 59, tea tree purple bud anthocyanidin 14, sodium carboxymethylcellulose 4, xylitol 13, citric acid 6,
Malic acid 16, Phyllanthus embical fruit extract 31, plant thalline crude extract 12, water 125.
4. functional drink according to claim 1, it is characterised in that by fresh passion flower-fruit peel wash clean, drying, powder
It is broken；In material-water ratio m:V=1:15,0.4%-0.8% pectase is added by the mass ratio of raw material, 22-38min is digested at 45 DEG C；
Destroy the enzyme treatment is carried out, filtering, leaves and takes filtrate.
5. functional drink according to claim 1, it is characterised in that the preparation method of the leaf of Moringa enzymolysis product
For：Fresh leaf of Moringa is cleaned, by leaf of Moringa and pure water according to m:V=1:5 ratio mixing, is blended using mixer, by original
The original quality of material adds 1.7%-2.5% pectase, reaches 3.5 using lemon acid for adjusting pH, 8- is digested at 50 DEG C
12min, after enzymolysis preliminary enzymolysis liquid, preliminary enzymolysis liquid is boiled into 10min enzyme deactivations, 50-55 DEG C is cooled to, using tripolyphosphate
The pH of preliminary enzymolysis liquid is adjusted to 6.5 by sodium, and 0.5%-0.9% compound fertilizer production, enzymolysis are added by the original quality of raw material
0.8-1.5h, enzymolysis boil 20min enzyme deactivations after terminating, and are filtered with the nylon cloth of 200 mesh, then be by molecular cut off by filtrate
5kDa device for ultrafiltration membrane filters, and obtains the leaf of Moringa enzymolysis product of clarification.
6. functional drink according to claim 1, it is characterised in that the preparation method of the Phyllanthus embical fruit extract is：
By Phyllanthus embical fruit and pure water according to m:V=1:20 ratio mixing, after boiling 30 min, will be cooled to the Phyllanthus embical fruit and water of room temperature
Mixture is added in colloid mill, and regulation gap is 0.1 mm, is ground three times, by the original quality of raw material than adding 0.5%-1.2%
Pectase, reach 3.5 using lemon acid for adjusting pH, digest 15-35min at 50 DEG C, after enzymolysis, enzymolysis liquid is boiled into 30 min
Enzyme deactivation, room temperature is cooled to, is filtered with the nylon cloths of 200 mesh, then by filtrate by molecular cut off be 2 kDa device for ultrafiltration membrane mistakes
Filter, obtain the Phyllanthus embical fruit extract solution of clarification.
7. functional drink according to claim 1, it is characterised in that the preparation method of plant thalline crude extract is：Take
The stem and leaf of fresh luffa vine are cleaned to not obvious impurity, after surface sterilization processing, are seeded to PDA and Gause I culture
2-5d is cultivated on base, then separation obtains bacterium colony；Picking inoculated by hypha block is in the cone equipped with fermentation medium from activated inclined plane
In shape bottle, 37 DEG C are put, 100r/min shaking table shaken cultivations 5d；Fermentation culture medium removes thalline, and filtrate is with isometric ethyl acetate
Extraction 3 times, merge organic phase, 50 DEG C are concentrated under reduced pressure and are evaporated, as mixed bacteria liquid crude extract；The thalline for centrifuging and being filtrated to get,
With appropriate 95% edible ethanol condensing reflux 3 times, merge ethanol phase, be concentrated under reduced pressure and be evaporated, as plant thalline crude extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711023082.1A CN107691922A (en) | 2017-10-26 | 2017-10-26 | A kind of functional drink |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1260965A (en) * | 1999-01-21 | 2000-07-26 | 陈哲超 | Process for preparing phyllanthus emblica powder preparation |
| KR101339706B1 (en) * | 2013-06-20 | 2013-12-10 | 재단법인 금산국제인삼약초연구소 | A compound for immune strengthen inclusion reducing the bitterness of red ginseng, the extract of immune, and the probiotics |
| CN104783248A (en) * | 2015-03-09 | 2015-07-22 | 李德新 | Passiflora edulis drink |
| CN106306936A (en) * | 2016-08-17 | 2017-01-11 | 深圳百绿盛农业科技发展有限公司 | Preparation method of moringa oleifera enzymolyzed drink |
| CN106616139A (en) * | 2016-08-31 | 2017-05-10 | 邓志程 | Children's functional drink |
-
2017
- 2017-10-26 CN CN201711023082.1A patent/CN107691922A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1260965A (en) * | 1999-01-21 | 2000-07-26 | 陈哲超 | Process for preparing phyllanthus emblica powder preparation |
| KR101339706B1 (en) * | 2013-06-20 | 2013-12-10 | 재단법인 금산국제인삼약초연구소 | A compound for immune strengthen inclusion reducing the bitterness of red ginseng, the extract of immune, and the probiotics |
| CN104783248A (en) * | 2015-03-09 | 2015-07-22 | 李德新 | Passiflora edulis drink |
| CN106306936A (en) * | 2016-08-17 | 2017-01-11 | 深圳百绿盛农业科技发展有限公司 | Preparation method of moringa oleifera enzymolyzed drink |
| CN106616139A (en) * | 2016-08-31 | 2017-05-10 | 邓志程 | Children's functional drink |
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