CN107691484A - A kind of natural plants antiseptic and preparation method - Google Patents
A kind of natural plants antiseptic and preparation method Download PDFInfo
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- CN107691484A CN107691484A CN201710689927.4A CN201710689927A CN107691484A CN 107691484 A CN107691484 A CN 107691484A CN 201710689927 A CN201710689927 A CN 201710689927A CN 107691484 A CN107691484 A CN 107691484A
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- tetraptera
- tetrapleura
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of natural plants Tetrapleura tetraptera antiseptics and preparation method.After its bark or tree root are fully cleaned, shearing, drying, with absolute ethyl alcohol, soak extraction, Tetrapleura tetraptera barks or the mass ratio of tree root and absolute ethyl alcohol are 1 in shaker incubator：After 9, soak extraction 24h, filter to get filtrate, be evaporated absolute ethyl alcohol in a rotary evaporator and obtain Tetrapleura tetraptera barks or tree root extract.Plant-type antibacterial agent of the present invention has broad spectrum antibacterial, and is sprayed directly on food, can reduce food quality deterioration, ensures the security of food, the shelf life of extension food, increase commodity value.The present invention can effectively reduce food apparatus, food processing environment foodborne bacterial pathogenses quantity, can be applied to the fields such as food fresh keeping, food antiseptic.
Description
Technical field
The present invention relates to a kind of natural plant extracts, refer in particular to a kind of Tetrapleura tetraptera extracts and its
Application in antiseptic is prepared, using effective antimicrobial component in natural plants Tetrapleura tetraptera, it is made
Safe and non-toxic natural plants antiseptic, the present invention be directed to the drug tolerant bacterias such as the foodborne bacterial pathogenses of field of food and MRSA
Antiseptic.
Background technology
Existing food antiseptic various types, the overwhelming majority use the organic antibacterial agent with certain toxicity, such as
Vanillic aldehyde or ethyl vanillin aldehydes compound.Under this background, field of food safety is badly in need of a kind of safe natural antibacterial agent,
And there is broad spectrum antibacterial.
Tetrapleura tetraptera are a kind of West Africa pea family flowering plants, have height edibility.Flower
It is yellow/pink, raceme is white, and fruit has dark brown.It is generally found the low land forest in Tropical Africa.Fruit is by meat
Matter pulp and small dark brown seed composition.Fruit has fragrance, the aromatic odor of feature, is attributed to the fact that its insect-repellent.It is used as
Spices and fragrance (foreign country's torrid zone smell).It is the mollusks killing medicine of Nigeria with one of plant, it can also be used to which management is taken out
Jerk, leprosy, inflammation and rheumatoid pain.This plant can be used to make spices, medicine, can also season dish, but
Application of the Tetrapleura tetraptera bark and tree root in food antibacterial field is not mentioned also.
The content of the invention
It is an object of the invention to provide a kind of Tetrapleura tetraptera extracts and its in food antibacterial field
In application, including Tetrapleura tetraptera bark extracts and Tetrapleura tetraptera tree roots extraction
Thing.
Described Tetrapleura tetraptera bark extracts, prepared using following method：Tetrapleura
After tetraptera barks fully clean, shearing, drying, with absolute ethyl alcohol in shaker incubator soak extraction,
The mass ratio of Tetrapleura tetraptera barks and absolute ethyl alcohol is 1：After 9, soak extraction 24h, filter to get filtrate,
Absolute ethyl alcohol is evaporated in rotary evaporator and obtains Tetrapleura tetraptera bark extracts.
Described Tetrapleura tetraptera tree root extracts, prepared using following method：Tetrapleura
After tetraptera tree roots fully clean, shearing, drying, with absolute ethyl alcohol in shaker incubator soak extraction,
The mass ratio of Tetrapleura tetraptera tree roots and absolute ethyl alcohol is 1:After 9, soak extraction 24h, filter to get filtrate,
Absolute ethyl alcohol is evaporated in rotary evaporator and obtains Tetrapleura tetraptera tree root extracts.
The natural plant type antiseptic of the present invention is Tetrapleura tetraptera extracts, and after 8h, it is sterilized
Rate has reached 99.9999%, it is shown that preferable bactericidal effect, and there is broad spectrum antibacterial.Tetrapleura
Tetraptera extracts not only can effectively kill foodborne bacterial pathogenses, still there is good resist to important pathogen MRSA
Bacterium effect.
Brief description of the drawings
Suppression of the table 1Tetrapleura tetraptera bark extracts to foodborne bacterial pathogenses and pathogenic bacteria of nosocomial infection
Bacterium effect and bactericidal effect.
Table 2Tetrapleura tetraptera tree roots are to foodborne bacterial pathogenses and the fungistatic effect of pathogenic bacteria of nosocomial infection
With bactericidal effect.
Antibacterial effect of Fig. 1 Tetrapleura tetraptera extracts to Escherichia coli.
Antibacterial effect of Fig. 2 Tetrapleura tetraptera extracts to staphylococcus aureus.
Antibacterial effect of Fig. 3 Tetrapleura tetraptera extracts to Escherichia coli biofilm.
Antibacterial effect of Fig. 4 Tetrapleura tetraptera extracts to Staphylococcus Aureus Biofilm.
Antibacterial effect of Fig. 5 Tetrapleura tetraptera extracts to MRSA biomembranes.
Embodiment
Illustrate the embodiment of the present invention, but the protection content of the present invention by Examples below, be not only limited to this
Embodiment 1
Measure of the Tetrapleura tetraptera extracts to pathogenic bacteria MIC and MBC：
1) experimental bacteria (the entitled italic of bacterium)
Food-borne pathogeny bacterium：
Escherichia coli (Escherichia coli IFO 3301)；
Enterorrhagia Bacillus coil 0157 (Escherichia coli 0157:H7)
Staphylococcus aureus (Staphylococcus aureus 30179)；
Bacterium enteritidis (Salmonella Enteritidis B11)；
Bacillus subtilis (Bacillus cereus IFO345)；
Listeria monocytogenes (Listeria monocytogenes M24-1)；
Pathogenic bacteria of nosocomial infection：
Methicillin-resistant staphylococcus aureus MRSA (Methicillin-resistant Staphylococcus
aureusT34)；
2) experimental method
Bacterium needed for this experiment is cultivated in advance.Tetrapleura tetraptera barks are added in NB culture mediums to carry
Thing is taken, the series concentration for then making Tetrapleura tetraptera bark extracts using twice of gradient dilution is respectively
0.16%th, 0.08%, 0.04%, 0.02%, 0.01%, addition is appropriate above-mentioned for examination bacterium, and it is 10 to make initial bacteria concentration5cfu/
ML, shaken cultivation.According to the muddy degree of fluid nutrient medium, judge Tetrapleura tetraptera bark extracts to each
The MIC (Minimum Inhibitory Concentrations) of bacterium, is carried containing minimum Tetrapleura tetraptera barks
Thing concentration and defecator are taken, as Tetrapleura tetraptera bark extracts are to the MIC for trying bacterium.It is and dense from MIC
Degree starts each concentration (MIC, 2MIC, 4MIC) clarification sample liquid and carries out plate streaking, and the flat board pulled is incubated, no for examination bacterium bacterium
It is born long Cmin, as Tetrapleura tetraptera bark extracts are to the MBC (Minimum for trying bacterium
bactericidal Concentrations).Here concentration refers to Tetrapleura tetraptera bark extracts
Percent by volume, in particular to Tetrapleura tetraptera bark extracts are diluted to required concentration with PBS.
Tetrapleura tetraptera tree root extracts are added in NB culture mediums, then utilize twice of gradient dilution
The series concentration for making Tetrapleura tetraptera root extracts is respectively 0.16%, 0.08%, 0.04%, 0.02%,
0.01%, addition is appropriate above-mentioned for examination bacterium, and it is 10 to make initial bacteria concentration5Cfu/mL, shaken cultivation.According to the muddy of fluid nutrient medium
Turbid degree, MIC of the Tetrapleura tetraptera tree roots extracts to each bacterium is judged, containing minimum Tetrapleura
Tetraptera root extracts concentration and defecator, as Tetrapleura tetraptera root extracts are to for trying bacterium
MIC.And each concentration (MIC, 2MIC, 4MIC) clarification sample liquid carries out plate streaking since MIC concentration, the flat board constant temperature training pulled
Support, no Cmin for examination bacterium colony growth, as Tetrapleura tetraptera tree roots extract is to for trying bacterium
MBC.Here concentration refers to the percent by volume of Tetrapleura tetraptera tree root extracts, in particular to incited somebody to action
Tetrapleura tetraptera tree roots extracts are diluted to required concentration with PBS.
3) experimental result
Inventive samples plant-type antibacterial agent, there is very strong antibacterial effect for above pathogen.As shown in Table 1
Tetrapleura tetraptera bark extracts are to 6 kinds of foodborne bacterial pathogenses and nosocomial infection bacterium MRSA minimal inhibitory concentrations
Scope is 0.01%-0.1%, and minimum bactericidal concentration scope is 0.01%-0.1%.Tetrapleura as shown in Table 2
Tetraptera tree roots are 0.01%- to 6 kinds of foodborne bacterial pathogenses and nosocomial infection bacterium MRSA minimal inhibitory concentrations scope
0.025%, minimum bactericidal concentration scope is 0.0125%-0.05%.Tetrapleura tetraptera bark extracts and
Tetrapleura tetraptera tree root extracts are respectively provided with broad spectrum antibacterial, and just have preferably sterilization effect in low dosage
Fruit.
Embodiment 2
Measure of the Tetrapleura tetraptera extracts to pathogenic bacteria bactericidal activity：
1) experimental bacteria (the entitled italic of bacterium)
Escherichia coli (Escherichia coli IFO 3301)；
Staphylococcus aureus (Staphylococcus aureus 30179)；
2) experimental method
In phosphate buffer [Phosphate-Buffered Saline, PBS, 0.03mol/L, pH (7.2-7.4)]
Middle addition finite concentration (1 MIC) Tetrapleura tetraptera extracts, and access for examination bacterium (105-106CFU/
ML), after shaken well, (150r/min) culture is vibrated in 37 DEG C of full warm air shaking tables, is determined with the method for plate culture count residual
Deposit bacterium number.
3) experimental result
As shown in Figure 1 and Figure 2, in Tetrapleura tetraptera bark extracts and Tetrapleura
After tetraptera tree roots extract effect 8h, Escherichia coli and staphylococcus aureus bacterium number are from 106-107CFU/mL drops to
Below 10CFU/mL, sterilizing rate reach 99.999%, it is shown that stronger bactericidal effect.
Embodiment 3
Antibacterial effect of the Tetrapleura tetraptera extracts to bacterial biof iotalm
1) experimental bacteria
Foodborne bacterial pathogenses：
Escherichia coli (Escherichia coli IFO 3301)；
Staphylococcus aureus (Staphylococcus aureus 30179)；
Pathogenic bacteria of nosocomial infection：
Methicillin-resistant staphylococcus aureus MRSA (Methicillin-resistant Staphylococcus
aureus T34)；
2) experimental method
By stainless steel iron plate (10mm × 10mm), ultrasound soaks 24h in absolute ethyl alcohol, after being cleaned up with distilled water,
Sterile stainless steel substrates are added the Tetrapleura tetraptera containing finite concentration (1 MBC) by 121 DEG C of sterilizing 15min
Experimental group is used as in the sterile TSB of bark extract, sterile stainless steel substrates are added in the TSB for not adding antiseptic as blank pair
According to.Access is for examination bacterium (10 in TSB4CFU/mL), (Escherichia coli cultivate 24h, golden yellow Portugal to 37 DEG C of quiescent cultures for a period of time
Grape coccus and MRSA cultures 48h).
Stainless steel substrates are taken out, with sterile PBS 3 times, is put into the test tube containing 1mLPBS, ultrasonic 10min, makes not
The biofilm detachment to become rusty on steel disc forms bacteria suspension.Ten times of gradient dilutions of 1mL bacteria suspensions that will be obtained, using NA in bacteria suspension
Viable count is measured.
By stainless steel iron plate (10mm × 10mm), ultrasound soaks 24h in absolute ethyl alcohol, after being cleaned up with distilled water,
Sterile stainless steel substrates are added the Tetrapleura tetraptera containing finite concentration (1 MBC) by 121 DEG C of sterilizing 15min
Experimental group is used as in the sterile TSB of tree root extract, sterile stainless steel substrates are added in the TSB for not adding antiseptic as blank pair
According to.Access is for examination bacterium (10 in TSB4CFU/mL), (Escherichia coli cultivate 24h, golden yellow Portugal to 37 DEG C of quiescent cultures for a period of time
Grape coccus and MRSA cultures 48h).Stainless steel substrates are taken out, with sterile PBS 3 times, are put into the test tube containing 1mLPBS,
Ultrasonic 10min, the biofilm detachment on stainless steel substrates is set to form bacteria suspension.The ten times of gradient dilutions of 1mL bacteria suspensions that will be obtained, profit
Viable count in bacteria suspension is measured with NA.
4) experimental result
Remaining viable count carries to evaluate Tetrapleura tetraptera in the biomembrane that this experiment is formed using measure
Take cleaning performance of the thing to biomembrane.According to Fig. 3,4,5 as can be seen that not made by Tetrapleura tetraptera extracts
48h biomembranes viable count on stainless steel substrates is 106-107CFU/cm2, extracted by Tetrapleura tetraptera
After thing effect, remaining viable count is 10 on Escherichia coli and staphylococcus aureus stainless steel3-104CFU/cm2, MRSA stainless steels
Upper remaining viable count is 104-105CFU/cm2, illustrate Tetrapleura tetraptera extracts to these three pathogenic bacteria
Biomembrane has preferable elimination effect.
Table 1
Table 2
Claims (5)
- A kind of 1. natural plants antiseptic, it is characterised in that：The natural plants antiseptic is Tetrapleura Tetraptera bark extracts, prepared using following method：After Tetrapleura tetraptera barks fully clean, Shearing, drying, with absolute ethyl alcohol in shaker incubator soak extraction, Tetrapleura tetraptera barks and anhydrous second The mass ratio of alcohol is 1：After 9, soak extraction 24h, filter to get filtrate, be evaporated absolute ethyl alcohol in a rotary evaporator and obtain Tetrapleura tetraptera bark extracts.
- A kind of 2. natural plants antiseptic, it is characterised in that：The natural plants antiseptic is Tetrapleura Tetraptera tree root extracts, prepared using following method：After Tetrapleura tetraptera tree roots fully clean, Shearing, drying, with absolute ethyl alcohol in shaker incubator soak extraction, Tetrapleura tetraptera tree roots and anhydrous second The mass ratio of alcohol is 1:After 9, soak extraction 24h, filter to get filtrate, be evaporated absolute ethyl alcohol in a rotary evaporator and obtain Tetrapleura tetraptera tree root extracts.
- A kind of 3. natural plants antiseptic as claimed in claim 1, it is characterised in that：To foodborne bacterial pathogenses and nosocomial infection Pathogen has good fungistatic effect and bactericidal effect, to foodborne bacterial pathogenses and nosocomial infection bacterium MRSA minimal inhibitory concentration models It is 0.01%-0.1% to enclose for 0.01%-0.1%, minimum bactericidal concentration scope.
- A kind of 4. natural plants antiseptic as claimed in claim 2, it is characterised in that：To foodborne bacterial pathogenses and nosocomial infection Pathogen has good fungistatic effect and bactericidal effect, to foodborne bacterial pathogenses and nosocomial infection bacterium MRSA minimal inhibitory concentration models It is 0.0125%-0.05% to enclose for 0.01%-0.025%, minimum bactericidal concentration scope.
- A kind of 5. natural plants antiseptic as described in claim 3 or 4, it is characterised in that：The foodborne bacterial pathogenses are big Enterobacteria (Escherichia coli IFO 3301)；Enterorrhagia Bacillus coil 0157 (Escherichia coli 0157: H7) staphylococcus aureus (Staphylococcus aureus 30179)；Bacterium enteritidis (Salmonella Enteritidis B11)；Bacillus subtilis (Bacillus cereus IFO345)；Listeria monocytogenes (Listeria monocytogenes M24-1)；The pathogenic bacteria of nosocomial infection is methicillin-resistant staphylococcus aureus MRSA(Methicillin-resistant Staphylococcus aureus T34)。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710689927.4A CN107691484A (en) | 2017-08-14 | 2017-08-14 | A kind of natural plants antiseptic and preparation method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710689927.4A CN107691484A (en) | 2017-08-14 | 2017-08-14 | A kind of natural plants antiseptic and preparation method |
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| Publication Number | Publication Date |
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| CN107691484A true CN107691484A (en) | 2018-02-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710689927.4A Pending CN107691484A (en) | 2017-08-14 | 2017-08-14 | A kind of natural plants antiseptic and preparation method |
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| Country | Link |
|---|---|
| CN (1) | CN107691484A (en) |
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2017
- 2017-08-14 CN CN201710689927.4A patent/CN107691484A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| O.K.ACHI 等: "Composition and antibacterial activities or Tetrapleura tetraptera Taub. Pod Extras", 《RESEARCH JOURNAL OF MICROBIOLOGY》 * |
| UCHECHI.N.EKWENYE 等: "ANTIBACTERIAL ACTIVITY OF TETRAPLEURA TETRAPTERATAUB.POD", 《INTERMATONAL JOURNAL OF PHARMA AND BIO SCIENCES》 * |
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Application publication date: 20180216 |