CN107691224A - A kind of seed propagation method of floribunda orchid - Google Patents
A kind of seed propagation method of floribunda orchid Download PDFInfo
- Publication number
- CN107691224A CN107691224A CN201711112013.8A CN201711112013A CN107691224A CN 107691224 A CN107691224 A CN 107691224A CN 201711112013 A CN201711112013 A CN 201711112013A CN 107691224 A CN107691224 A CN 107691224A
- Authority
- CN
- China
- Prior art keywords
- floribunda
- orchid
- culture
- explant
- leaf juice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The present invention provides a kind of seed propagation method of floribunda orchid, belongs to technical field of plant propagation.This method includes the steps such as sterilization, Fiber differentiation, squamous subculture and the strong seedling culture of explant using the capsule of floribunda orchid as explant, modes of reproduction.Moringa leaf juice and matrimony vine leaf juice are with the addition of in culture medium used in each cultivation stage as nutritional agents.Wherein, the composition of inducing culture is：VW+ chlorethanol 0.8mg/L 1.2mg/L+ agar 5.0g/L 8.0g/L+ sucrose 2.0g/L 4.0g/L+ Moringa leaf juice 5.0g/L 8.0g/L+ matrimony vine leaf juice 3.0g/L 5.0g/L.Culture medium prescription provided by the invention is remarkably improved induction differentiation rate, explant growth coefficient and the rooting rate of floribunda orchid, shortens cultivation cycle, improves the quality of floribunda orchid tissue-cultured seedling.
Description
【Technical field】
The present invention relates to technical field of plant propagation, and in particular to a kind of seed propagation method of floribunda orchid.
【Background technology】
Floribunda orchid (Cymbidium floribundum) is that orchid family Cymbidium grows nonparasitically upon another plant class plant, category《Endangered species of wild fauna and flora kind
International trade pact》Appendix II species, it is China second class protection plant.Floribunda orchid is distributed mainly on Yunnan, Guangxi, Guangdong, good fortune
Each province on the south Jian Deng China the Changjiang river；It is born in height above sea level 100-3300m woods, on border tree, or along trench palisades.Its leaf banding pattern
Keratin, there is gloss, the cold-proof power of drought resisting is strong；Lace fragrance, the florescence is longer, and pattern is rich and flowery colourful, has higher ornamental value,
It is one of comparatively ideal pot flowers.
Floribunda orchid bud easily breaks up, and florescence length, cultivation is easy, and growth is fast, strong adaptability, is good ornamental flower.It is long
Since phase, floribunda orchid is based on division propagation, but reproduction speed is slow.Want to obtain more plant, Ke Yili in a short time
Bred with the mode of tissue cultures, the domestic report to floribunda orchid progress tissue culture propagation is seldom.At present, also there are some scholar's research
Explant is done using floribunda orchid capsule carry out tissue cultures and obtain the method for floribunda orchid seedling, but because the research of correlation is less,
The culture medium for being more suitable for the growth of stem apex differentiated tissue is not found, is caused in tissue culture procedures, cultivation cycle length, cultivates effect
It is unsatisfactory.
【The content of the invention】
The goal of the invention of the present invention is：For above-mentioned problem, there is provided a kind of seed propagation method of floribunda orchid,
Culture medium prescription provided by the invention is remarkably improved induction differentiation rate, explant growth coefficient and the rooting rate of floribunda orchid, contracting
Short culture cycle attaining, improve the quality of floribunda orchid tissue-cultured seedling.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of seed propagation method of floribunda orchid, include sterilization, Fiber differentiation, squamous subculture and the strong sprout training of explant
Support, concretely comprise the following steps：
(1) sterilization of explant：The capsule after floribunda orchid pollination is gathered as explant, is rinsed first with flowing water, then use
Volumetric concentration is 75% alcohol-pickled 1-2min, then with the HgCl that mass concentration is 0.1%2Liquid disinfectant 10-15 minutes, use
Aseptic water washing 2-4 times, fruit both ends are cut, split fruit, the seed in fruit is used to be inoculated with；
(2) Fiber differentiation：Seed in the fruit is uniformly sprinkling upon in inducing culture, is 25 ± 1 DEG C in temperature, light
According to for 2000LX, cultivated 50-55 days under conditions of daily illumination 10-14h, obtain floribunda orchid garden bulb；The inducing culture
Form and be：VW+ chlorethanol 0.8mg/L-1.2mg/L+ agar 5.0g/L-8.0g/L+ sucrose 2.0g/L-4.0g/L+ Moringa leaf juices
5.0g/L-8.0g/L+ matrimony vine leaf juices 3.0g/L-5.0g/L；
(3) squamous subculture：The floribunda orchid garden bulb that growth obtains in step (2) is transferred in subculture medium, in temperature
Spend for 25 ± 1 DEG C, cultivate under conditions of illumination 2000LX, daily illumination 10-14h, constantly changed with the growth of garden bulb
Culture medium, squamous subculture obtain the floribunda orchid shoot of long root long leaf after 80-85 days；
(4) strong seedling culture：Floribunda orchid shoot is transferred in strong seedling culture base, it is 3-5 bars to cultivate to radical, blade 3-4
Piece, height of seedling 4-8cm, that is, obtain the floribunda orchid seedling that can be transplanted.
In the present invention, it is preferable that the composition of the subculture medium is:MS+ activated carbon 1g/L+ methyl α-naphthyl acetates 2mg/L-3mg/
L+ agar 5.0g/L-8.0g/L+ Moringa leaf juice 5.0g/L-8.0g/L+ matrimony vine leaf juice 3.0g/L-5.0g/L+ sucrose 2.0g/L-
4.0g/L。
In the present invention, it is preferable that the composition of the strong seedling culture base is:1/2MS+ activated carbon 1g/L+ indolebutyric acids
0.4mg/L-0.6mg/L+ agar 5.0g/L-8.0g/L+ Moringa leaf juice 5.0g/L-8.0g/L+ matrimony vine leaf juices 3.0g/L-5.0g/L
+ sucrose 2.0g/L-4.0g/L.
In the present invention, it is preferable that the preparation method of the Moringa leaf juice and matrimony vine leaf juice is：It is then abundant first to clean blade
Smash to pieces, then with filtered through gauze, the filtrate be corresponding juice.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
Moringa leaf juice and matrimony vine leaf juice are with the addition of in the culture medium that the present invention uses as nutriment, is contained in leaf of Moringa
Several mineral materials, vitamin, 20 kinds of amino acid, 46 kinds of antioxygen elements and 36 kinds of naturally anti-scorching bodies and mineral matter, every 100 grams of Moringa
In the vitamin C that contains be 7 times of citrus, iron is 3 times of spinach, and vitamin A is 4 times of carrot, and calcareous is 4 times of milk,
Potassium is 3 times of banana, and protein is 2 times of Yoghourt；Folium lycii is rich in glycine betaine, rutin and several amino acids and trace element
Deng the joint addition of, both materials, worked with growth regulator, basal medium one, floribunda orchid can be significantly improved
Differentiation rate, explant growth coefficient and rooting rate are induced, shortens cultivation cycle, and improve the quality of floribunda orchid tissue-cultured seedling.
【Embodiment】
In order to more clearly express the present invention, below by way of specific embodiment, the invention will be further described.
In an embodiment of the present invention, Moringa leaf juice, the preparation method of matrimony vine leaf juice are as follows：
Moringa leaf juice：First clean and blade and then fully smash to pieces, then with filtered through gauze, the filtrate be Moringa leaf juice.
Matrimony vine leaf juice：First clean and blade and then fully smash to pieces, then with filtered through gauze, the filtrate be Moringa leaf juice.
In an embodiment of the present invention, culture medium prepares is transferred to 5.4- according to the method for prior art by pH value afterwards
5.6.
Embodiment 1
A kind of seed propagation method of floribunda orchid, is carried out using following steps：
(1) sterilization of explant：The capsule after floribunda orchid pollination is gathered as explant, is rinsed first with flowing water, then use
Volumetric concentration is 75% alcohol-pickled 1min, then with the HgCl that mass concentration is 0.1%2Liquid disinfectant 10 minutes, use is sterile
Water is rinsed 2 times, and fruit both ends are cut, and splits fruit, and the seed in fruit is used to be inoculated with；
(2) Fiber differentiation：Seed in the fruit is uniformly sprinkling upon in inducing culture, is 25 ± 1 DEG C in temperature, light
According to for 2000LX, cultivated 55 days under conditions of daily illumination 10h, obtain floribunda orchid garden bulb；The composition of the inducing culture is：
VW+ chlorethanol 0.8mg/L+ agar 5.0g/L-8.0g/L+ sucrose 2.0g/L+ Moringa leaf juice 5.0g/L+ matrimony vine leaf juices 5.0g/L；
(3) squamous subculture：The floribunda orchid garden bulb that growth obtains in step (2) is transferred in subculture medium, it is described
The composition of subculture medium is:MS+ activated carbon 1g/L+ methyl α-naphthyl acetate 2mg/L+ agar 5.0g/L+ Moringa leaf juice 5.0g/L+ folium lyciis
Juice 5.0g/L+ sucrose 2.0g/L；It is 25 ± 1 DEG C in temperature, is cultivated under conditions of illumination 2000LX, daily illumination 10h, with
Culture medium is constantly changed in the growth of garden bulb, and squamous subculture obtains the floribunda orchid shoot of long root long leaf after 85 days；
(4) strong seedling culture：Floribunda orchid shoot is transferred in strong seedling culture base, the composition of the strong seedling culture base is:1/
2MS+ activated carbon 1g/L+ indolebutyric acid 0.4mg/L+ agar 5.0g/+ Moringa leaf juice 5.0g/L+ matrimony vine leaf juice 5.0g/L+ sucrose
2.0g/L；Culture is 3-5 bars to radical, blade 3-4 pieces, height of seedling 4-8cm, that is, obtains the floribunda orchid seedling that can be transplanted.
Embodiment 2
A kind of seed propagation method of floribunda orchid, is carried out using following steps：
(1) sterilization of explant：The capsule after floribunda orchid pollination is gathered as explant, is rinsed first with flowing water, then use
Volumetric concentration is 75% alcohol-pickled 1.5min, then with the HgCl that mass concentration is 0.1%2Liquid disinfectant 12 minutes, with nothing
Bacterium water is rinsed 3 times, and fruit both ends are cut, and splits fruit, and the seed in fruit is used to be inoculated with；
(2) Fiber differentiation：Seed in the fruit is uniformly sprinkling upon in inducing culture, the group of the inducing culture
Turn into：VW+ chlorethanol 1.0mg/L+ agar 6.0g/L+ sucrose 3.0g/L+ Moringa leaf juice 7.0g/L+ matrimony vine leaf juices 4.0g/L；
Temperature is 25 ± 1 DEG C, is cultivated 50 days under conditions of illumination 2000LX, daily illumination 12h, obtains floribunda orchid garden bulb；
(3) squamous subculture：The floribunda orchid garden bulb that growth obtains in step (2) is transferred in subculture medium, it is described
The composition of subculture medium is:MS+ activated carbon 1g/L+ methyl α-naphthyl acetate 2.5mg/L+ agar 6.0g/L+ Moringa leaf juice 7.0g/L+ matrimony vines
Leaf juice 4.0g/L+ sucrose 3.0g/L；It is 25 ± 1 DEG C in temperature, is cultivated under conditions of illumination 2000LX, daily illumination 12h, with
Culture medium is constantly changed in the growth for garden bulb, and squamous subculture obtains the floribunda orchid shoot of long root long leaf after 80 days；
(4) strong seedling culture：Floribunda orchid shoot is transferred in strong seedling culture base, the composition of the strong seedling culture base is:1/
2MS+ activated carbon 1g/L+ indolebutyric acid 0.5mg/L+ agar 6.0g/L+ Moringa leaf juice 6.0g/L+ matrimony vine leaf juice 4.0g/L+ sucrose
3.0g/L；Culture is 3-5 bars to radical, blade 3-4 pieces, height of seedling 4-8cm, that is, obtains the floribunda orchid seedling that can be transplanted.
Embodiment 3
A kind of seed propagation method of floribunda orchid, is carried out using following steps：
(1) sterilization of explant：The capsule after floribunda orchid pollination is gathered as explant, is rinsed first with flowing water, then use
Volumetric concentration is 75% alcohol-pickled 2min, then with the HgCl that mass concentration is 0.1%2Liquid disinfectant 15 minutes, use is sterile
Water is rinsed 4 times, and fruit both ends are cut, and splits fruit, and the seed in fruit is used to be inoculated with；
(2) Fiber differentiation：Seed in the fruit is uniformly sprinkling upon in inducing culture, is 25 ± 1 DEG C in temperature, light
According to for 2000LX, cultivated 52 days under conditions of daily illumination 14h, obtain floribunda orchid garden bulb；The composition of the inducing culture is：
VW+ chlorethanol 1.2mg/L+ agar 8.0g/L+ sucrose 4.0g/L+ Moringa leaf juice 8.0g/L+ matrimony vine leaf juices 3.0g/L；
(3) squamous subculture：The floribunda orchid garden bulb that growth obtains in step (2) is transferred in subculture medium, it is described
The composition of subculture medium is:MS+ activated carbon 1g/L+ methyl α-naphthyl acetate 2.5mg/L+ agar 8.0g/L+ Moringa leaf juice 8.0g/L+ matrimony vines
Leaf juice 3.0g/L+ sucrose 4.0g/L；It is 25 ± 1 DEG C in temperature, is cultivated under conditions of illumination 2000LX, daily illumination 14h, with
Culture medium is constantly changed in the growth for garden bulb, and squamous subculture obtains the floribunda orchid shoot of long root long leaf after 82 days；
(4) strong seedling culture：Floribunda orchid shoot is transferred in strong seedling culture base, the composition of the strong seedling culture base is:1/
2MS+ activated carbon 1g/L+ indolebutyric acid 0.6mg/L+ agar 8.0g/L+ leaf of Moringa 8.0g/L+ matrimony vine leaf juice 3.0g/L+ sucrose
4.0g/L；Culture is 3-5 bars to radical, blade 3-4 pieces, height of seedling 4-8cm, that is, obtains the floribunda orchid seedling that can be transplanted.
Comparative example 1
Moringa juice and matrimony vine leaf juice are replaced using banana puree, other conditions are same as Example 2, i.e. the group of inducing culture
Turn into：VW+ chlorethanol 1.0mg/L+ agar 6.0g/L+ sucrose 3.0g/L+ banana purees 10g/L；The composition of subculture medium is：MS
+ activated carbon 1g/L+ methyl α-naphthyl acetate 2.5mg/L+ agar 6.0g/L+ banana puree 10g/L+ sucrose 3.0g/L；The composition of strong seedling culture base
For 1/2MS+ activated carbon 1g/L+ indolebutyric acid 0.5mg/L+ agar 6.0g/L+ banana puree 10g/L+ sucrose 3.0g/L；Using implementation
The identical method culture floribunda orchid seedling of example 2, statistics induction differentiation rate, explant growth coefficient and rooting rate in incubation.
Comparative example 2
Different from the culture medium used in embodiment 2, specific culture medium is：The composition of inducing culture is：1/2MS+ lives
Property charcoal 1g/L+6-BA 1.0mg/L+NAA0.1mg/L+ agar 6g/L+ sucrose 20g/L；The composition of subculture medium is：1/2MS+
Activated carbon 1g/L+6-BA 1.0mg/L+NAA0.5mg/L+ agar 6g/L+ sucrose 20g/L；The composition of strong seedling culture base is：1/
2MS+ activated carbon 1g/L+6-BA 1.0mg/L+NAA0.5mg/L+ agar 6g/L+ sucrose 20g/L.Using the identical side of embodiment 2
Method culture floribunda orchid seedling, statistics induction differentiation rate, explant growth coefficient and rooting rate in incubation.
Comparative example 3
Different from the culture medium used in embodiment 2, specific culture medium is：The composition of inducing culture is：VW+ activated carbons
1g/L+6-BA 1.0mg/L+NAA0.1mg/L+ agar 6g/L+ sucrose 20g/L；The composition of subculture medium is：MS+ activated carbons
1g/L+6-BA 1.0mg/L+NAA0.5mg/L+ agar 6g/L+ sucrose 20g/L；The composition of strong seedling culture base is：1/2MS+ activity
Charcoal 1g/L+6-BA 1.0mg/L+NAA0.5mg/L+ agar 6g/L+ sucrose 20g/L.It is more using the identical method culture of embodiment 2
Hua Lanmiao, statistics induction differentiation rate, explant growth coefficient and rooting rate in incubation.
Tissue culture test method and result：
Every group takes 200 explant samples, and the method that embodiment 1-3 and comparative example 1-3 is respectively adopted carries out tissue culture, will
The explant Fiber differentiation of floribunda orchid counts the differentiation and proliferation situation of each group explant, by the protocorm of Fiber differentiation after 55 days
After squamous subculture 85 days, statistical average inductivity and average rooting rate；Continuation strong seedling culture statistical average root long after 65 days, respectively
The result of group is as shown in table 1.
The tissue culture growing state result of table 1
From the results shown in Table 2, relative to comparative example 1, the average bud of embodiment 1-3 average stem length, growth coefficient
Differentiation rate, average rooting rate, average root long are obvious preferable, illustrate to compare addition banana puree, using Moringa leaf juice and folium lycii
Juice is engaged as nutritional agents, the composition of culture medium is more suitable for the growth of floribunda orchid explant.It is real relative to comparative example 2,3
A 1-3 is applied because the culture medium used is different, the growing state in each stage is also obvious preferable.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, the equal change completed or modification change under the technical spirit suggested by all present invention, all should belong to
Cover the scope of the claims in the present invention.
Claims (4)
1. a kind of seed propagation method of floribunda orchid, include sterilization, Fiber differentiation, squamous subculture and the strong seedling culture of explant,
It is characterized in that concretely comprise the following steps：
(1) sterilization of explant：The capsule after floribunda orchid pollination is gathered as explant, is rinsed first with flowing water, then use volume
Concentration is 75% alcohol-pickled 1-2min, then with the HgCl that mass concentration is 0.1%2Liquid disinfectant 10-15 minutes, use are sterile
Water is rinsed 2-4 time, and fruit both ends are cut, and splits fruit, and the seed in fruit is for being inoculated with；
(2) Fiber differentiation：Seed in the fruit is uniformly sprinkling upon in inducing culture, is 25 ± 1 DEG C in temperature, illumination is
Cultivated 50-55 days under conditions of 2000LX, daily illumination 10-14h, obtain floribunda orchid garden bulb；The composition of the inducing culture
For：VW+ chlorethanol 0.8mg/L-1.2mg/L+ agar 5.0g/L-8.0g/L+ sucrose 2.0g/L-4.0g/L+ Moringa leaf juices 5.0g/
L-8.0g/L+ matrimony vine leaf juices 3.0g/L-5.0g/L；
(3) squamous subculture：The floribunda orchid garden bulb that growth obtains in step (2) is transferred in subculture medium, is in temperature
25 ± 1 DEG C, cultivate under conditions of illumination 2000LX, daily illumination 10-14h, culture is constantly changed with the growth of garden bulb
Base, squamous subculture obtain the floribunda orchid shoot of long root long leaf after 80-85 days；
(4) strong seedling culture：Floribunda orchid shoot is transferred in strong seedling culture base, it is 3-5 bars to cultivate to radical, blade 3-4 pieces, seedling
High 4-8cm, that is, obtain the floribunda orchid seedling that can be transplanted.
2. the seed propagation method of floribunda orchid according to claim 1, it is characterised in that：The composition of the subculture medium
For:MS+ activated carbon 1g/L+ methyl α-naphthyl acetate 2mg/L-3mg/L+ agar 5.0g/L-8.0g/L+ Moringa leaf juice 5.0g/L-8.0g/L+ Chinese hollys
Qi leaf juice 3.0g/L-5.0g/L+ sucrose 2.0g/L-4.0g/L.
3. the seed propagation method of floribunda orchid according to claim 1, it is characterised in that：The composition of the strong seedling culture base
For:1/2MS+ activated carbon 1g/L+ indolebutyric acid 0.4mg/L-0.6mg/L+ agar 5.0g/L-8.0g/L+ Moringa leaf juices 5.0g/L-
8.0g/L+ matrimony vine leaf juice 3.0g/L-5.0g/L+ sucrose 2.0g/L-4.0g/L.
4. the seed propagation method of floribunda orchid according to claim 1, it is characterised in that：The Moringa leaf juice and folium lycii
The preparation method of juice is：First clean and blade and then fully smash to pieces, then with filtered through gauze, the filtrate be corresponding juice.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711112013.8A CN107691224A (en) | 2017-11-13 | 2017-11-13 | A kind of seed propagation method of floribunda orchid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711112013.8A CN107691224A (en) | 2017-11-13 | 2017-11-13 | A kind of seed propagation method of floribunda orchid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107691224A true CN107691224A (en) | 2018-02-16 |
Family
ID=61178448
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711112013.8A Withdrawn CN107691224A (en) | 2017-11-13 | 2017-11-13 | A kind of seed propagation method of floribunda orchid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107691224A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113796315A (en) * | 2021-10-13 | 2021-12-17 | 湖南文理学院 | Quick propagation method of endangered wild plant cymbidium floribundum |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104285804A (en) * | 2014-10-24 | 2015-01-21 | 柳州市天姿园艺有限公司 | Special culture medium for cymbidium floribundum |
| CN106857258A (en) * | 2017-02-27 | 2017-06-20 | 中国农业科学院蔬菜花卉研究所 | A kind of quick breeding method for tissue culture blue with leaf pocket |
-
2017
- 2017-11-13 CN CN201711112013.8A patent/CN107691224A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104285804A (en) * | 2014-10-24 | 2015-01-21 | 柳州市天姿园艺有限公司 | Special culture medium for cymbidium floribundum |
| CN106857258A (en) * | 2017-02-27 | 2017-06-20 | 中国农业科学院蔬菜花卉研究所 | A kind of quick breeding method for tissue culture blue with leaf pocket |
Non-Patent Citations (6)
| Title |
|---|
| 丁长春等: "多花兰的离体萌发和试管成苗技术研究", 《安徽农业科学》 * |
| 唐凤鸾等: "多花兰种子无菌萌发及离体快繁研究", 《广西植物》 * |
| 张永清等: "《药用植物栽培学》", 28 February 2013, 中国中医药出版社 * |
| 王郑昊等: "多花兰种子繁育技术研究", 《山东林业科技》 * |
| 谢宇等: "《精编神农本草经彩色图鉴》", 31 August 2016, 海南出版社 * |
| 赖来展等: "《黑色食品与健康长寿》", 31 October 2014, 金盾出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113796315A (en) * | 2021-10-13 | 2021-12-17 | 湖南文理学院 | Quick propagation method of endangered wild plant cymbidium floribundum |
| CN113796315B (en) * | 2021-10-13 | 2022-05-17 | 湖南文理学院 | Quick propagation method of endangered wild plant cymbidium floribundum |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101611697B (en) | Virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19' | |
| CN101647393B (en) | Fast tissue culture reproducing method of actinidia eriantha | |
| CN107047320B (en) | A kind of bigflower centranthera root method for tissue culture | |
| CN103190347B (en) | Teapot dates tissue culturing method | |
| CN102283129B (en) | Method for inducing and multiplying prothallium of Huperzia serrata | |
| CN101595824B (en) | Rapid in-vitro seedling raising method by utilizing sandalwood seed embryo | |
| CN102870680A (en) | Efficient rapid propagation technique appropriate for detoxified rabbiteye blueberries | |
| CN101836585B (en) | Tissue-culture seedling raising method of rhodiola crenulata | |
| CN104285813A (en) | Camellia chrysantha tissue culture propagation method | |
| CN102228003B (en) | Culture method for brassica oleracea L. var. acephala microspore regeneration plant | |
| CN100429972C (en) | Tissue culture propagation process of late spring michelia | |
| CN101695280B (en) | Tissue culture and rapid propagation method of raspberries | |
| CN101874472A (en) | Tissue culture method of Pitaya | |
| CN102138532A (en) | Technique for breeding seedlings of Taihe tribute Chinese toon by tissue culture | |
| CN103947548A (en) | Method for establishing agapanthus high-frequency regeneration system | |
| CN105638465A (en) | Strawberry tissue culture fast propagation method | |
| CN108243959A (en) | It is a kind of using yellow fine strain of millet wood stem section as the highly efficient regeneration method of explant | |
| CN106818488A (en) | A kind of blue quick breeding method for tissue culture of valve pocket long | |
| CN1284444C (en) | Sterile seeding and tissue cultivating technology for Vanda | |
| CN106818481A (en) | A kind of kohlrabi quick breeding method for tissue culture | |
| CN103039363A (en) | Rooting medium for tissue culture seedling propagation of camellia oleifera abel and propagation method thereof | |
| CN103039362A (en) | Subculture medium for tissue culture seedling propagation of camellia oleifera abel and propagation method thereof | |
| CN103416302A (en) | Method for culturing regeneration plant of somatic embryo of osmanthus fragrans Lour | |
| CN107691224A (en) | A kind of seed propagation method of floribunda orchid | |
| CN101743909B (en) | Tissue culture and propagation method of ''Haierlian'' of Illiciaceae plant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180216 |