CN107684636A - A kind of bactericidal composition and as the bacteria cellulose antimicrobial composite material obtained by it - Google Patents
A kind of bactericidal composition and as the bacteria cellulose antimicrobial composite material obtained by it Download PDFInfo
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- CN107684636A CN107684636A CN201610633180.6A CN201610633180A CN107684636A CN 107684636 A CN107684636 A CN 107684636A CN 201610633180 A CN201610633180 A CN 201610633180A CN 107684636 A CN107684636 A CN 107684636A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/425—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/18—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
Abstract
The invention provides a kind of bactericidal composition, by weight including 5~20 parts of bacteria celluloses, 1~3 part of bioactive glass particle and 0~10 part of polymeric biomaterial.Present invention also offers a kind of bacteria cellulose antimicrobial composite material being prepared by the bactericidal composition.Bactericidal composition provided by the invention combines bacteria cellulose with bioactivity glass, new antimicrobial composite material can be obtained, it possesses several microns to hundreds of microns of porous, spongelike structure, thus there is more preferable cellular affinity, excellent water imbibition and absorption speed, but also there is certain bacteriostasis, available for prevention and treatment II degree, III degree burn or the wound for scalding the chronic refractory conjunction such as secondary trauma surface infestation and diabetes, there is very extensive medical usage.
Description
Technical field
The present invention relates to biomedical materials field, more particularly to a kind of bactericidal composition and thin as one kind obtained by it
Fungin antimicrobial composite material.
Background technology
Bacteria cellulose is one of focus of biological investigation of materials outside Now Domestic.As a kind of novel biomaterial, carefully
Fungin is close with natural (plant) cellulose on physical property, chemical composition and molecular structure, but has traditional day
The incomparable advantage of right cellulose.These properties are：(1) high-purity, the degree of polymerization and crystallinity.With plant cellulose phase
Than, without lignin, pectin, hemicellulose and other cell wall constituents, molecularly oriented is good, and structure is homogeneous, and with single fibre
Dimension form is present, and very high purity (reaches more than 99%).(2) hyperfine network structure.Passed through by diameter 3-4nm microfiber bundle
The filament band that hydrogen bond is interconnected to form, its width are about 30-100nm, thickness 3-8nm, are intertwined to form prosperity
Hyperfine network structure.(3) high-tensile and modulus of elasticity.(4) moisture holding capacity is strong.Due to superfine nano structure and molecule
It is interior there is substantial amounts of hydrophilic radical, have a lot " ducts " inside cellulose, surface area is 300 times of plant cellulose, is made
It has strong water suction and water holding moisture-retaining capacity, can absorb the moisture content of 60-400 times of its dry weight under normal circumstances.(5) higher life
Thing compatibility, adaptability and good biodegradability.The pure cellulose nanofiber synthesized by acetobacter forms, almost
Do not cause foreign matter and inflammatory reaction, there is good intensity and formed in situ, biocompatibility under hygrometric state, acid, micro-
Can directly it be degraded in nature under the conditions of biology and cellulase catalytic etc., it is free from environmental pollution, it is environmental friendly product.Carefully
Fungin good biocompatibility, intensity are high, have good hydrophily and ventilative water permeability, now useful bacteria cellulose
The commodity of the wound dressing such as artificial skin, gauze, bandage and " bandage " are made, it is mainly characterized by under moisture conditions mechanical
Intensity is high, has good permeability to gas, moisture and electrolyte, good with the compatibility of skin, nonirritant, structure is extremely thin
It is close, bacterium infection can be prevented, is advantageous to skin histology growth.
But fine and closely woven microstructure also constrains the application of bacteria cellulose, bacteria cellulose sheet to a certain extent
Body has nanofibrous structures, and these nanofibers are closely packed together, and the hole of formation is very small, is unfavorable for cell
Creep and breed.Moreover, bacteria cellulose does not have antibacterial activity in itself, it is impossible to prevents wound infection, therefore scientists are developed
Bacteria cellulose anti-biotic material containing antibiotic and argentiferous.Yet with drug resistance caused by the improper use of antibiotic with
And caused by the cytotoxicity brought of silver ion or elemental silver and silver precipitation the shortcomings of skin darkening so that above-mentioned anti-biotic material
Using receiving larger limitation.
Bioactivity glass has good biocompatibility and biocidal property, also has preferably to burn and chronic wound care
The effect of, comprehensive to weigh, repair field in wound has bigger advantage than antibiotic and Ag-containing compound.
The content of the invention
The defects of to overcome existing bacteria cellulose material to exist, an object of the present invention are to provide a kind of antibacterial combination
Thing, available for preparing new bacteria cellulose composite material.
It is a further object of the present invention to provide a kind of bacteria cellulose antimicrobial composite material.
Bactericidal composition provided by the invention includes 5~20 parts of bacteria celluloses, 1~3 part of bioactivity glass by weight
Glass particle and 0~10 part of polymeric biomaterial.
Preferably, bactericidal composition provided by the invention includes 8~10 parts of bacteria celluloses, 1~3 part of biology by weight
Activity glass particle and 1~5 part of polymeric biomaterial.It is compound that the addition of polymeric biomaterial can further change gained
The microstructure of material, improves the mechanical strength of composite, and increases the biocompatibility of composite.Wherein, it is common
Polymeric biomaterial for biomedical sector is all applied to the present invention, it may for example comprise but be not limited to collagen, gelatin,
Chitosan etc..
In bactericidal composition provided by the invention, the particle diameter of the bioactive glass particle is 5~100nm, is preferably
10~50nm, more preferably 15~30nm.
In bactericidal composition provided by the invention, the bacteria cellulose can derive from commercially available prod or made products, its
For micron particles, preferable particle size is 10~200 μm, more preferably 50~100 μm.
Bacteria cellulose antimicrobial composite material provided by the invention is as the antibacterial combination described in above any one of technical scheme
Thing is prepared.
Preferably, the antimicrobial composite material can be prepared into diversified forms as needed, preferably be prepared as antimicrobial compound film.
The preparation process of the antimicrobial composite material is：By the solution containing bioactive glass particle with containing bacterium fibre
The gel homogeneous suspension liquid mixing of crude granule is tieed up, foaming agent and plasticizer is added, forms mixed liquor, produced after drying.
Various forms can be made according to existing process according to being actually needed in composite prepared by said process.
In above-mentioned preparation process, the gel homogeneous suspension liquid containing bacterial fibers crude granule is by by bacteria cellulose
Formed after film is broken, wherein, the particle diameter of the bacterial fibers crude granule is 10~200 μm, preferably 50~100 μm.It is described thin
Fungin particle is preferred before broken to carry out purification process, to remove the thalline of residual, culture medium etc., and washs in neutrality.
In above-mentioned preparation process, the foaming agent is selected from NaCl, NaHCO3、Na2SO4、NH4HCO3, soluble polysaccharide, poly- second
One or more in glycol, polyvinyl alcohol；One or more of the plasticizer in glycerine, ISDN.Institute
State foaming agent, the dosage of plasticizer can be according to being actually needed by those skilled in the art's simple adjustment.
Above-mentioned preparation process also includes adding crosslinking agent and activator into the homogeneous mixture, adds crosslinking agent and work
Composite can be further promoted to form tridimensional network after agent, the mechanical performance of gained composite can also obtain bigger
Improve.Wherein, the crosslinking agent is selected from 1- ethyls -3- (3- dimethyl aminopropyls) carbodiimide hydrochloride, 1- dimethylaminos third
Base ethyl carbodiimide, cyclohexyl methyl morpholine ethyl carbodiimide β-butylene hydrosulphate, phenyleneethyl carbodiimide or
1.6- hexamethylenes (carbodiimide)；The activator is selected from n-hydroxysuccinimide or N- hydroxies succinyl is sub-
Amine.The dosage of the crosslinking agent and activator can be according to being actually needed by those skilled in the art's simple adjustment.
Bactericidal composition provided by the invention combines bacteria cellulose with bioactivity glass, can obtain new resist
Bacterium composite, it possesses several microns to hundreds of microns of porous, spongelike structure, thus with suitable hole size, high
Porosity and the pore morphology being connected, thus there is more preferable cellular affinity.Composite provided by the invention is advantageous to carefully
The formation of the adhesion of born of the same parents, propagation and extracellular matrix, be advantageous to cell and obtain nutrition and discharge metabolic waste, can meet normally
The cell metabolism tissue new with formation.Structure after change has higher specific surface area and suitable surface physics and chemistry
Can, being more beneficial for growth factor-loaded grade has the medicine of bioactivity and function.
Secondly as porous, the spongy microstructure of composite, can make it possess excellent water imbibition and water suction speed
Degree, available for absorbing wound hydrops and possessing certain anastalsis, so as to also have the function that certain promotion wound healing.
3rd, because the catabolite of bioactivity glass has alkalescent, so as to make composite have necessarily
Bacteriostasis, the deficiency of single bacteria cellulose material is compensate for, it is thin with existing carried with antibiotics available for the wound of infection
Fungin material is compared, cytotoxicity then can be low compared to that will not develop immunity to drugs with the bacteria cellulose material of carrying silver ion
A lot.
In summary, composite of the invention has excellent mechanical property, anti-microbial property and well permeable, saturating
Gas or drainage, moisture sorption effect, the surface of a wound dry, incrustation and healing can be promoted, available for prevention and treatment II degree, III degree burn or
Person scalds the wound of the chronic refractory conjunctions such as secondary trauma surface infestation and diabetes, has very extensive medical usage.
Brief description of the drawings
Fig. 1 is the SEM image of bioactivity glass/bacteria cellulose composite material prepared by embodiment 5；
Fig. 2 is the cell bio-activity chart of bioactivity glass/bacteria cellulose composite material of MTT experiment measure.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, the example of the present invention will be further described below
The technical scheme of property embodiment.
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.Wherein, agents useful for same, material
Deng being if not otherwise specified commercially available prod, the operation is this area routine operation if not otherwise specified.
The purifying of preparation example bacteria cellulose
Commercially available bacteria cellulose film is taken repeatedly to be rinsed with clear water, except striping surface medium and impurity.It is soaked in again
In 0.1mol/L NaOH solution, 60min is boiled at 80-100 DEG C, the thalline in bacteria cellulose film is washed out and residual is cultivated
Base.Among bacteria cellulose film is soaked in into 0.05mol/L NaOH solution again, 30min is boiled at 80-100 DEG C, then with steaming
Distilled water is repeatedly rinsed.
By the bacteria cellulose film after above-mentioned processing by disinfection by ultraviolet light, reach sterilization procedure requirement (subsequent technique
It is required that sterile working).By distilled water immersion 3-5 times of the bacteria cellulose film after above-mentioned sterilization, surveyed with the light press mold of pH test paper
PH value, the pH value for finally controlling bacteria cellulose film is 7.2, obtains bacteria cellulose after purification.
The bacterial cellulose wet-coating of gained is beaten to form the gel containing bacterial fibers crude granule by mechanical means
Homogeneous suspension liquid, for following examples.
Embodiment 1
The bioactive glass particle that 100 gram particle footpaths are 5 nanometers is dispersed in 500g water at room temperature and forms solution,
The bacteria cellulose graininess gel homogeneous suspension liquid (containing 900 grams of bacteria cellulose) of purification process is mixed again, grain diameter
50 μm of average out to, add 50g NaHCO3Foaming agent and 50g glycerine, homogeneous mixed liquor is made after disperseing by refiner；Will be equal
Mix liquid to be filtered under diminished pressure, bioactivity glass/bacteria cellulose composite material is isolated after freeze-drying.
Embodiment 2
At room temperature by 200 gram particle footpaths be 10 nanometers of bioactive glass particle be dispersed in 500g water formed it is molten
Liquid, then the bacteria cellulose graininess gel homogeneous suspension liquid (containing 800 grams of bacteria cellulose) of purification process is mixed, particle
100 μm of footpath average out to, 15gNaCl foaming agents and 15g ISDNs are added, be made after being disperseed by refiner and mix
Liquid；Homogeneous mixed liquor is filtered under diminished pressure, bioactivity glass/bacteria cellulose composite material is isolated after freeze-drying.
Embodiment 3
At room temperature by 100 gram particle footpaths be 15 nanometers of bioactive glass particle be dispersed in 500g water formed it is molten
Liquid, then bacteria cellulose graininess gel homogeneous suspension liquid (containing 900 grams of bacteria cellulose) and 1000g glue by purification process
Original solution mixes, 150 μm of grain diameter average out to, adds 20g sucrose foaming agent and 20g ISDNs, passes through refiner
Homogeneous mixed liquor is made after scattered；Homogeneous mixed liquor is filtered under diminished pressure, it is fine that bioactivity glass/bacterium is isolated after freeze-drying
Tie up element/collagen composite materials.Membranaceous composite is prepared after hot pressure reaction.
Embodiment 4
At room temperature by 200 gram particle footpaths be 20 nanometers of bioactive glass particle be dispersed in 500g water formed it is molten
Liquid, then the bacteria cellulose graininess gel homogeneous suspension liquid (containing 1000 grams of bacteria cellulose) and 500g of purification process is bright
Sol solution mixes, 50 μm of grain diameter average out to, adds 51g polyethylene glycol foaming agent and 51g ISDNs, passes through homogenate
Homogeneous mixed liquor is made after machine is scattered；Homogeneous mixed liquor is filtered under diminished pressure, bioactivity glass/bacterium is isolated after freeze-drying
Cellulose/gelatin-compounded material.Membranaceous composite is prepared after hot pressure reaction.
Embodiment 5
The bioactivity glass that 200 gram particle footpaths are 15 nanometers is dispersed in 500g water at room temperature and forms solution, then
The bacteria cellulose graininess gel homogeneous suspension liquid (containing 1000 grams of bacteria cellulose) and 500g collagens of purification process is molten
Liquid mixes, 100 μm of grain diameter average out to, adds the agent of 136g polyvinyl alcohol foams and 85g ISDNs, passes through refiner
Homogeneous mixed liquor is made after scattered；Add 1g carbodiimides (EDC) and 1g n-hydroxysuccinimides (NHS) are crosslinked, will
Homogeneous mixed liquor is filtered under diminished pressure, and bioactivity glass/bacterial cellulose/collagen composite material is isolated after freeze-drying.
Embodiment 6
The bioactivity glass that 200 gram particle footpaths are 15 nanometers is dispersed in 500g water at room temperature and forms solution, then
The bacteria cellulose graininess gel homogeneous suspension liquid (containing 1000 grams of bacteria cellulose) and 500g gelatin of purification process is molten
Liquid mixes, 50 μm of grain diameter average out to, adds 17g NaHCO3Foaming agent and 17g ISDNs, pass through refiner point
Homogeneous mixed liquor is made after dissipating；Add 1g carbodiimides (EDC) and 1g n-hydroxysuccinimides (NHS) are crosslinked, will be equal
Mix liquid to be filtered under diminished pressure, bioactivity glass/bacteria cellulose/gelatin composite is isolated after freeze-drying.
Test case
According to Fig. 1, it can be seen that, the bacteria cellulose of the gained of embodiment 5 has sponge with bioactivity glass composite
Shape, micrometer level porous structure, thus it can be inferred that its to be advantageous to cell proliferation, biocompatibility more preferable.
The composite of embodiment 5 is subjected to MTT experiment, negative control is blank, and positive control is 0.1% phenol.With
ELIASA determines absorbance (i.e. OD values) in 570nm wavelength, and the value can represent cytoactive, as a result as shown in Fig. 2 explanation is thin
Fungin and bioactivity glass composite cytotoxicity very little.
In addition, the composite that embodiment 1-6 is obtained carries out hemolytic experiment, the hemolysis rate for obtaining composite is followed successively by
2.1%th, 2%, 1.8%, 1.9%, 1.7%, 1.7%, and (hemolysis rate be less than 5% can testimonial material will not cause haemolysis), can
See the good biocompatibility that the composite has.
Composite prepared by the present invention is substantially better than common bacteria cellulose dressing on absorption speed and water absorption,
As shown in the result of table 1.Because bacteria cellulose/bioactivity glass composite of the present invention has micrometer level porous property (ginseng
Examine Fig. 1), therefore it has excellent water absorbing properties, and also absorption speed is also very fast, within the 10 second initial time, composite wood
The absorption speed of material is more than 20 times of conventional bacteria cellulose dressing, and it can absorb the moisture of own wt hundreds of times, is to pass
More than 4 times of system bacteria cellulose dressing.These behavior extensions clinical practice field of composite so that bacteria cellulose
Dressing may apply on the wound with sepage, quick to absorb wound exudate and keep the moisture state of wound, promote wound
Mouth healing.Simultaneously because the composite has higher specific surface area, quick water absorbing properties and larger water absorption so that
The material also has certain anthemorrhagic performance.
Table 1
| Absorption speed | Water absorption | |
| Conventional bacteria cellulose dressing | 0.02ml/s | 30ml |
| The composite of embodiment 1 | 0.4ml/s | 120ml |
| The composite of embodiment 3 | 0.3ml/s | 125ml |
| The composite of embodiment 5 | 0.3ml/s | 120ml |
Table 2 gives the ultimate tensile strength value of the composite of different embodiments, it can thus be seen that the present invention's is thin
Because microstructure changes, mechanical strength has declined fungin/bioactivity glass composite.Add macromolecule
After material (collagen, gelatin etc.), almost identical mechanical strength can be reached with the bacteria cellulose film of routine.Crosslinking agent is added to enter
After row crosslinking, mechanical performance further improves, even better than existing bacteria cellulose dressing, it can be seen that, of the invention answers
Condensation material is not only excellent beneficial to cell propagation, water absorbing properties, can also easily change its mechanical performance, thus can further expand
The application of big composite.
Table 2
| Title | Stretch Nmax |
| Conventional bacteria cellulose dressing | 28.3N |
| The composite of embodiment 1 | 10.2N |
| The composite of embodiment 3 | 20.5N |
| The composite of embodiment 5 | 25.2N |
| The composite of embodiment 6 | 26.0N |
Although in order to illustrate the present invention, the preferred embodiments of the invention, those skilled in the art are had been disclosed for
Member can do it should be appreciated that in the case where not departing from the present inventive concept and scope that claims are limited to the present invention
Go out various modifications, addition and replace.
Claims (10)
1. a kind of bactericidal composition, it is characterised in that by weight including 5~20 parts of bacteria celluloses, 1~3 part of bioactivity
Glass particle and 0~10 part of polymeric biomaterial.
2. bactericidal composition according to claim 1, it is characterised in that by weight including 8~10 parts of bacteria celluloses,
1~3 part of bioactive glass particle and 1~5 part of polymeric biomaterial.
3. bactericidal composition according to claim 1 or 2, it is characterised in that the particle diameter of the bioactive glass particle
For 5~100nm, preferably 10~50nm, more preferably 15~30nm.
4. bactericidal composition according to claim 1 or 2, it is characterised in that the polymeric biomaterial be selected from collagen,
One or more in gelatin, chitosan.
5. a kind of bacteria cellulose antimicrobial composite material, it is characterised in that as the antibacterial combination described in claim any one of 1-4
It is prepared by thing.
6. antimicrobial composite material according to claim 5, it is characterised in that the antimicrobial composite material is that antibacterial is compound
Film.
7. antimicrobial composite material according to claim 5, it is characterised in that the preparation process of the antimicrobial composite material
For：Solution containing bioactive glass particle is mixed with the gel homogeneous suspension liquid containing bacterial fibers crude granule, added
Foaming agent and plasticizer, mixed liquor is formed, produced after drying.
8. antimicrobial composite material according to claim 7, it is characterised in that the gel containing bacterial fibers crude granule
Homogeneous suspension liquid by by bacteria cellulose film it is broken after formed, wherein, the particle diameter of the bacterial fibers crude granule is 10~200
μm, preferably 50~100 μm.
9. antimicrobial composite material according to claim 7, it is characterised in that the foaming agent is selected from NaCl, NaHCO3、
Na2SO4、NH4HCO3, soluble polysaccharide, polyethylene glycol, the one or more in polyvinyl alcohol；The plasticizer be selected from glycerine,
One or more in ISDN.
10. antimicrobial composite material according to claim 7, it is characterised in that the preparation process is also included to described equal
Crosslinking agent and activator are added in phase mixture；Wherein, the crosslinking agent is selected from 1- ethyls -3- (3- dimethyl aminopropyls) carbon two
Inferior amine salt hydrochlorate, 1- dimethyl aminopropyls ethyl carbodiimide, cyclohexyl methyl morpholine ethyl carbodiimide β-butylene sulphation
Thing, phenyleneethyl carbodiimide or 1.6- hexamethylenes (carbodiimide)；It is sub- that the activator is selected from N- hydroxysuccinimidyls acyl
Amine or N- hydroxy thiosuccinimides.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111840633A (en) * | 2019-04-29 | 2020-10-30 | 上海硅健生物材料有限公司 | Skin repairing film and preparation method thereof |
| CN113456884A (en) * | 2020-03-30 | 2021-10-01 | 北京纳通医学科技研究院有限公司 | Composite bionic patch and preparation method thereof |
| CN117244096A (en) * | 2023-10-08 | 2023-12-19 | 华东交通大学 | Boron-silicon-based bioactive glass modified bacterial cellulose functional dressing and preparation method thereof |
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| CN111840633A (en) * | 2019-04-29 | 2020-10-30 | 上海硅健生物材料有限公司 | Skin repairing film and preparation method thereof |
| CN111840633B (en) * | 2019-04-29 | 2022-08-23 | 上海硅健生物材料有限公司 | Skin repairing film and preparation method thereof |
| CN113456884A (en) * | 2020-03-30 | 2021-10-01 | 北京纳通医学科技研究院有限公司 | Composite bionic patch and preparation method thereof |
| CN117244096A (en) * | 2023-10-08 | 2023-12-19 | 华东交通大学 | Boron-silicon-based bioactive glass modified bacterial cellulose functional dressing and preparation method thereof |
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