CN1076830A - A kind of agricultural or horticultural germicide - Google Patents

Abstract

The invention provides a kind of to the effective agricultural or horticultural bactericide of pathogenic mold fungi such as control rice blast fungus etc., its with the derivative that contains albocycline (Albocycline) and at least a above albocycline thereof as active ingredient.These effective ingredients can make by cultivating streptomycete sp.RS-31 bacterial strain.

Description

A kind of agricultural or horticultural germicide

The present invention relates to a kind of is the agricultural or horticultural bactericide of effective ingredient with albocycline (Albocycline) and derivative thereof.

Generally mostly be organic phosphor as hyphomycetic disinfectant use in agriculture of pathogenic such as preventing and kill off rice blast.The heterocyclic organic compound, but all there are problems such as drug effect, poisoning and generation resistance bacterium in this class medicament.For this reason, the object of the present invention is to provide a kind ofly to have anti-pathogenic mold fungi activity, and can be developed into active compound into disinfectant use in agriculture.

Albocycline and derivative thereof are as the known [K.Harada etc. of compound with antibacterial activity, The Journal of Antibiotics, Vol, 27, No, 10,1187-1197(1984)], but it has the supression effect to pathogenic mold fungis such as Pyricularia oryzaes and but belongs to head and know.The inventor etc. have found that according to the result of test albocycline and derivative thereof have the activity of resisting rice blast bacteria, and with the derivative of albocycline and at least a above albocycline thereof the effective ingredient as disinfectant use in agriculture, thereby finished the present invention.

Below just the present invention be described in detail.

Albocycline is the compound with following structural formula, and has multiple derivative.

Disinfectant use in agriculture of the present invention is an effective ingredient to contain albocycline and the derivative of one or two or more kinds thereof, can carry out illustration with following compound as the derivative of albocycline.Albocycline M-1(8,9-epoxy body), albocycline M-2(12-hydroxyl body), albocycline M-5(11-hydroxyl body), albocycline M-7(10-hydroxyl body), albocycline M-4(18-hydroxyl body), albocycline M-3(11-hydroxyl, 2,3-dihydro body), albocycline M-6(10-hydroxyl, 2,3-dihydro body).

Above-mentioned albocycline and derivative thereof can adopt known method to produce easily, in addition, also can be with streptomycete sp.(Streptomyces sp.) the RS-31 bacterial strain places C4(that glucose 2%, soluble starch 1%, meat extract 0.1%, dry yeast 0.4%, soybean meal 2.5%, salt 0.2% are adjusted to PH=7.2 after being dissolved in distilled water) medium, cultivated 3-5 days at 25-30 ℃, produce with separation means such as solvent extractions again.Above-mentioned streptomycete sp.RS-31 bacterial strain was the microorganism of adopting in the soil by Yixing, Jiangsu Province, China, and had delivered China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation (preserving number is CGMCC No.0173) on March 26th, 1992.

Streptomycete sp.RS-31 bacterial strain has following mycology property, and because of containing L in its cell wall, the L-diaminopimelic acid is so can identify the microorganism that it belongs to streptomyces.

The cultivation proterties of bacterium

(1) starch yeast agar medium

Growth: good

Aerial hyphae: short

The aerial hyphae tone: the 4ec(cow-bezoar)/a(is white)

Tone in the base: 2pg(is dark yellow)

Soluble pigment: do not have

(2) yeast extract Fructus Hordei Germinatus soaks the juice agar medium

Growth: good

Aerial hyphae: short

The aerial hyphae tone: a(is white)

Tone in the base: 3pg(is yellowish-brown)

Soluble pigment: do not have

(3) oatmeal agar medium

Growth: good

Aerial hyphae: common

Aerial hyphae tone: 4ea(is bright apricot yellow)

Tone: 2ic(is light golden yellow in the base)

Soluble pigment: do not have

(4) starch mineral salt agar medium

Growth: common

Aerial hyphae: few

Aerial hyphae tone: the 4ec(cow-bezoar)

Tone in the base: 2gc(pumpkin Huang)

Soluble pigment: do not have

(5) tyrosine agar medium

Growth: common

Aerial hyphae: short

Aerial hyphae tone: the 13dc(pearl)

Tone in the base: the 3pe(amber)

Soluble pigment: 3pn(is palm fibre secretly)

(6) sucrose nitrate agar medium

Growth: common

Aerial hyphae: common

Aerial hyphae tone 4ca(digested tankage is red)

Tone: 3gc(is bright orange brown in the base)

Soluble pigment: do not have

(7) glucose asparagine agar medium

Growth: bad

Aerial hyphae: lack/almost do not have

The aerial hyphae tone: a(is white)

Tone in the base: 2ba(is greyish white)

Soluble pigment: do not have

(8) glycerine asparagine agar medium

Growth: bad

Aerial hyphae: do not have

Tone in the base: 2ba(is greyish white)

Soluble pigment: do not have

(9) nutrient agar

Growth: common

Aerial hyphae: few

Aerial hyphae tone 5ba(shell is red)

Tone in the base: 2na(is bright orange)

Soluble pigment: do not have

(10) peptone yeast extract iron agar medium

Growth: bad

Aerial hyphae: few

The red ash of aerial hyphae tone 3ih()

Tone in the base: 2pg(is dark yellow)

Soluble pigment: the 3po(ebenine)

3, Tang utilization

D-glucose is grown up good ++

D-fructose is grown up good ++

The growth of L-rhamnose-

The raffinose growth-

The D-wood sugar is grown up good ++

L-arabinose is grown up good ++

The sucrose growth-

The inositol growth-

D-mannitol is grown up good ++

The D-lactose is grown up good ++

Albocycline and derivative thereof can directly be used as disinfectant use in agriculture, also can with the use that is mixed of other pesticide adjuvants.There is no particular restriction to its preparation, for example can be made into various forms such as emulsion, wetting powder, pulvis, granula subtilis, granule, tablet, finish, spray, aerosol.For improving the result of use of disinfectant use in agriculture, increase stability, dispersiveness etc., can use suitable insecticides adjuvant, as carrier (thinner), spreader-sticker, emulsifier, wetting agent, dispersant, the agent etc. of collapsing.

Can make aromatic hydrocarbonses such as water, toluene, dimethylbenzene as the liquid carrier; Alcohols such as methyl alcohol, butanols, ethanol; Ketones such as acetone; Amide-types such as dimethyl formamide; Sulfoxide classes such as dimethyl sulfoxide (DMSO); Methyl naphthalene, cyclohexane, vegetable and animals oils, fatty acid etc.

Can use clay, kaolin, talcum powder, diatomite, silica, calcium carbonate, montmorillonite, bentonite, feldspar, quartz, alumina, sawdust, nitrocellulose, starch, gum Arabic etc. as the solid carrier.

During as emulsifier, dispersant, can add surfactant commonly used, as anionic species surfactants such as the sulfuric ester of higher alcohol, hard ester acyl trimethylamine chloride, polyoxyethylene phenylate, dodecyl trimethylaminyl ethlyl lactones; The cationic surfactant; Nonionic class surfactant and zwitterionic classes surfactant.

Can use polyoxyethylene nonyl phenylate, polyoxyethylene lauryl ether etc. as spreader-sticker.Can use polyoxyethylene nonyl phenylate, dialkyl sulfo-succinate etc. as wetting agent.Can use carboxymethyl cellulose, polyvinyl alcohol etc. as curing agent.Agent can be used lignin sodium carbonate, NaLS etc. as collapsing.

The content of the effective ingredient of disinfectant use in agriculture of the present invention (albocycline and derivative thereof) can be according to the form of preparation, and application process etc. are different and decide.For example: when the emulsifier, can effective ingredient 10-50, part solvent 10-80 part, surfactant 3-20 part (weight ratio), preparation or mother liquor.When actual using, be diluted with water to again fixed concentration.When the wetting powder, can effective ingredient 5-80 part, solid carrier 10-90 part, surfactant 1-20 part (weight ratio) is mixed with female powder, when actual using, be diluted with water to again fixed concentration.Can effective ingredient 1-5 part when the pulvis, solid carrier 95-99 part (weight ratio) directly is mixed with pulvis and uses.Can effective ingredient 5-15 part when the granule, solid carrier 82-94 part, surfactant 1-3 part (weight ratio) directly is mixed with particle and uses.

Disinfectant use in agriculture of the present invention also can mix use with other bactericide, Insecticides (tech) ﹠ Herbicides (tech), fertilizer, soil conditioner, miticide etc. except that need contain the compound of effective ingredient.

Disinfectant use in agriculture of the present invention can adopt the whole bag of tricks such as cauline leaf distribution and used for ponds to use.Usage amount when cauline leaf scatters is the solution of 100-200 litre 20-1000PPm for common per 10 ares.Usage amount during used for ponds is the granule of 1-10 kilogram 5-15% for common per 10 ares.

According to the present invention, a kind of novel agricultural or horticultural bactericide just can be provided.Below the present invention will be described in more detail with regard to use-case.

The preparation of embodiment 1(albocycline)

Production bacterium streptomycin sp.(Streptomyces sp. with albocycline) RS-31 bacterial strain (CGMCC No.0173) places and 70mlC4(is housed with glucose 2%, soluble starch 1%, meat extract 0.1%, dry yeast 0.4%, soybean meal 2.5%, after salt 0.2% is dissolved in distilled water, adjust to PH=7.2) in 20 K1 flasks of medium, cultivated 4 days in 28 ℃.Thalline and culture fluid are opened in centrifugation then, extract thalline with the 800ml70% acetone solvent, and the acetone soln of gained merges with culture fluid behind concentrating under reduced pressure.Use 1 liter ethyl acetate extraction three times again, can obtain the antibacterial substance more than 90%.With ethyl acetate extraction thing concentrating under reduced pressure, behind dried the consolidating, use molten Jie of a spot of methyl alcohol again, then at silica gel tlc post (Merck corporate system) (solvent: benzene: launch ethyl acetate=1: 1), obtain active region composition (the Rf value is about 0.58).Product is again with HPLC(capsule C18,80% methyl alcohol) refining after, get final product the albocycline about 30mg, in addition, available 1H-NHR of the evaluation of product and photodiode are carried out, and can confirm that product is an albocycline.

The mensuration (in vitro) of embodiment 2(resisting rice blast bacteria activity)

(1) detects plate

Pyricularia oryzae (Pyricuraria oryzae) was cultivated for two weeks in 28 ℃ in potato-sucrose medium, the spore of gained joins in the agar medium, and double-layer solidifies to make and detects plate.

(2) mensuration of antimicrbial power

The albocycline acetone soln of 40 μ l variable concentrations is put into the paper disc that dry sterilization is crossed, air-dry.Paper disc placed detect the top of plate, measure the diameter of restraining circle around the paper disc after 3 days in 28 ℃ of cultivations.Its result is as shown in table 1.

The determination of activity of embodiment 3(resisting rice blast bacteria) (in vivo)

(1) test plants

Adopt after planting in the 3.5 leaf phase paddy rice (love know the rising sun) of growth after 12 days in the air-conditioner temperature (daytime is the highest 28 ℃, minimum 20 ℃ of night, humidity 60%), every potted plant rice 10 strains.

(2) medicament uses

Molten Jie tests medicament with acetone, add distilled water and 1 spreader-sticker and make spreading liquid, and to adjust the spreading liquid ultimate density is 500PPm that the ultimate density of acetone is below 5%.With the blasticidin-S S of 20PPm medicament in contrast.Test plants placed on the turntable while rotating with sprayer scatter, at room temperature air-dry then.

Inoculation and morbidity are checked

Inoculation source is used in 28 ℃ of Pyricularia oryzaes (Pyricuraria oryzae) after cultivating for 2 weeks on the oatmeal plate medium, and spore concentration is 15 * 10 ⁵Individual/milliliter.In giving the inoculating hood that is positioned over earlier in the greenhouse, inoculate.Inoculation was placed in 25 ℃ of dark greenhouses 24-48 hour, take out then plant in 25 ℃ with the artificial irradiation of mercury vapor lamp 1 day half.Inoculate back 5 days and detect scab, preventive effect can be calculated as follows, and its result is as shown in table 1.

Preventive effect (%)=(1-(the scab sum of test region)/(the scab sum in non-processor district)) * 100

Table 1

Albocycline in vitro in vivo

Concentration (PPm) is restrained circle (mm) preventive effect (%)

100 21 93.7

50 16 77.8

25 15 46.0

12.5 14 -

Claims (1)

1, a kind of agricultural or horticultural bactericide is characterized in that containing in its effective ingredient the derivative of albocycline (Alboycline) and at least a above albocycline.