CN107677653A - Three-dimensional image forming apparatus and imaging method based on two-photon fluorescence - Google Patents
Three-dimensional image forming apparatus and imaging method based on two-photon fluorescence Download PDFInfo
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- CN107677653A CN107677653A CN201710734908.9A CN201710734908A CN107677653A CN 107677653 A CN107677653 A CN 107677653A CN 201710734908 A CN201710734908 A CN 201710734908A CN 107677653 A CN107677653 A CN 107677653A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
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- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The present invention relates to two-photon fluorescence imaging, a kind of three-dimensional image forming apparatus based on two-photon fluorescence is provided, including laser and tank, also include fluorescence excitation light path and fluorescence reception light path, fluorescence excitation light path includes focus adjustment block, fluorescence reception light path includes detector, and it is provided with the first lens in the light path between laser and focus adjustment block, the second lens are provided with light path between focus adjustment block and tank, the 3rd lens are provided with the light path between optical filter and detector；A kind of imaging method is also provided.The laser of the present invention sends light and sends in light tank focal position in fluorescent dye by focus adjustment block regulation after diverging, and pass through mobile exciting light focus position in fluorescent dye, absorbed in the focal position two-photon for sending light, and then produce two-photon fluorescence, the excitation wavelength of two-photon fluorescence is longer, with very deep penetration depth, arbitrary three-dimensional fluorescence image can be internally formed in fluorescent dye.
Description
Technical field
The present invention relates to two-photon fluorescence imaging, more particularly to a kind of three-dimensional image forming apparatus based on two-photon fluorescence and
Imaging method.
Background technology
The process of two-photon fluorescence excitation, be exactly ground state fluorescence molecule and meanwhile absorb two photons, from ground state transition to swash
State is sent out, then by relaxation process, gives off fluorescent photon.Single photon fluorescence is that the fluorescence launched will than excitation wavelength
It is long, and two-photon fluorescence is then that the fluorescence launched is shorter than excitation wavelength.
Two-photon excitation is a non-linear process, small different from requirement of the Single Photon Absorption to optical density, only in light
Strong sufficiently large focal point is just enough to produce two-photon excitation, so fluorescence excitation only produces in focal point.But current three
Dimension imaging device is difficult to obtain required three-dimensional fluorescence image.
The content of the invention
It is an object of the invention to provide a kind of three-dimensional image forming apparatus based on two-photon fluorescence, it is intended to existing for solving
Technology be difficult to obtain three-dimensional fluorescence image the problem of.
What the present invention was realized in：
The embodiment of the present invention provides a kind of three-dimensional image forming apparatus based on two-photon fluorescence, including laser and can place
The tank of fluorescent dye, in addition to fluorescence excitation light path and fluorescence reception light path, the fluorescence excitation light path include adjustable
Laser sends the focus adjustment block of optical focus position in fluorescent dye, and the fluorescence reception light path is caused including that can receive
The detector of fluorescence, and the first lens are provided with the light path between the laser and the focus adjustment block, it is described
Fluorescence reception light path is provided with optical filter in the light path between the tank and the detector, in the focus adjustment block
The second lens are provided with light path between the tank, are set in the light path between the optical filter and the detector
There are the 3rd lens.
Further, the focus adjustment block includes spatial light modulator and xy scanning galvanometers, and the spatial light is adjusted
Device processed is used for the position for adjusting exciting light longitudinal focusing point in fluorescent dye, and the xy scanning galvanometers are used to change focus point
Lateral attitude, the spatial light modulator be arranged in parallel with the xy scanning galvanometers.
Further, it is provided with dichroic in the light path between the spatial light modulator and the xy scanning galvanometers
Mirror, the dichroscope is parallel to the xy scanning galvanometers, and the detector is located on the reflected light path of the dichroscope.
Further, the spatial light modulator receives the incident light that the laser is sent.
Further, the laser is femto-second laser, centre wavelength 800nm, pulsewidth 300fs, repetition rate
76MHz。
The embodiment of the present invention also provides a kind of three-D imaging method based on two-photon fluorescence, comprises the following steps：
In placing fluorescent dye in tank, laser sends light and enters fluorescence excitation light path, is flat through the diverging of the first lens
Row light is incident to focus adjustment block；
The focus adjustment block reflection sends light, and sends light through the fluorescent dye in the second lens entrance to tank
On, produce fluorescence；
Fluorescence enters fluorescence reception light path after the diverging of the second lens, and is filtered through optical filter in the fluorescence reception light path
Veiling glare, and fluorescence through the 3rd lens enter detector in；
Change the focal position on fluorescent dye by adjusting the focus adjustment block, appointed with obtaining in fluorescent dye
The three-dimensional fluorescence image of one position.
Further, the focus adjustment block includes spatial light modulator and xy scanning galvanometers, parallel after diverging
Light is incident in the spatial light modulator, is reflexed to through the spatial light modulator on the xy scanning galvanometers, and described in warp
Second lens are incident to after the reflection of xy scanning galvanometers.
The invention has the advantages that：
In the imaging device of the present invention, the light that sends of laser sends light water after diverging by focus adjustment block regulation
Focal position in groove in fluorescent dye, and by the position of mobile exciting light focus point in fluorescent dye, can be in fluorescence
Dyestuff is internally formed arbitrary three-dimensional fluorescence image, and fluoroscopic image enters in fluorescence reception light path after being excited, and fluorescence
Optical filter on receiving light path can filter veiling glare thereon so that only fluorescence using detector by that and then can be connect
The fluorescence passed through is received, i.e., three-dimensional fluorescence image can be obtained by detector.In the imaging method of the present invention, light is being sent
Focal position two-photon is absorbed, and then produces two-photon fluorescence, and the excitation wavelength of two-photon fluorescence is longer, is had very deep
Penetration depth, therefore fluoroscopic image can be formed in bigger space, facilitate detector to obtain.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the required accompanying drawing used in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the light channel structure schematic diagram of the three-dimensional image forming apparatus provided in an embodiment of the present invention based on two-photon fluorescence.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained all other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
Referring to Fig. 1, the embodiment of the present invention provides a kind of three-dimensional image forming apparatus based on two-photon fluorescence, including laser 1
And tank 2, wherein laser 1 is mainly used for sending laser, and tank 2 is then to be used to place fluorescent dye, and laser 1 is sent out
The laser gone out can produce fluorescence when on the fluorescent dye being incident in tank 2, and imaging device also includes fluorescent exciting
Road 3 and fluorescence reception light path 4, the light that sends of laser 1 are exposed on fluorescent dye after fluorescence excitation light path 3, and it is produced
Raw fluorescence then enters in fluorescence reception light path 4, and then can be acquired, and specifically, fluorescence excitation light path 3 includes focus tune
Component 5 is saved, and the first lens 6 are provided with the light path between focus adjustment block 5 and laser 1, what laser 1 was sent swashs
Light is incident to focus adjustment block 5 after the diverging of the first lens 6, and after focus adjustment block 5 can adjust the diverging of the first lens 6
The locus of directional light, longitudinal direction and lateral adjustments can be carried out, and be incident to the second lens 7 after regulation is suitable and gathered
Jiao, focal position are located on fluorescent dye, and fluorescence reception light path 4 includes that the detector 8 of caused fluoroscopic image can be received, i.e.,
Fluoroscopic image caused by fluorescent dye receives to be received by detector 8 in light path into fluorescence, and fluorescence reception light path 4 is in tank 2
Optical filter 9 is provided with light path between detector 8, the optical filter 9 is shortwave optical filter 9, for only allowing required fluorescence
It is imaged into detector 8, other veiling glares, including exciting light is blocked in outside, to improve the signal to noise ratio of system, generally
The 3rd lens 10 are provided with light path between optical filter 9 and detector 8, can be by the fluorescent foci of diverging to detector 8
It is interior.In the present invention, the light that sends of laser 1 sends fluorescent dye in light tank 2 after diverging by the regulation of focus adjustment block 5
In focal position, and by the position of mobile exciting light focus point in fluorescent dye, in the double light in focal position for sending light
Son is absorbed, and then produces two-photon fluorescence, and the excitation wavelength of two-photon fluorescence is longer, has very deep penetration depth, can
To be internally formed arbitrary three-dimensional fluorescence image in fluorescent dye, and fluoroscopic image enters fluorescence reception light path 4 after being excited
It is interior, and the optical filter 9 in fluorescence reception light path 4 can filter veiling glare thereon so that only fluorescence is by that and then can adopt
The fluorescence that passes through is received with detector 8, i.e., three-dimensional fluorescence image can be obtained by detector 8, compared to Single Photon Absorption, its
Two-photon absorption occurs over just focal point, efficiently avoid in fluorescent dye the dry of caused spuious fluorescence on exciting light path
Disturb.Femto-second laser 1, the wherein a length of 800nm of cardiac wave, pulsewidth 300fs, repetition rate 76MHz can be used for laser 1,
Femtosecond laser can be produced.
Optimize above-described embodiment, refine the structure of focus adjustment block 5, it includes spatial light modulator 51 and xy scannings
Galvanometer 52, its spatial light modulator 51 is used to adjust exciting light longitudinal focusing point position in fluorescent dye, and xy scannings are shaken
Mirror 52 then can be used for adjusting focus point lateral attitude, and this is adjusted by the cooperation of spatial light modulator 51 and xy scanning galvanometers 52
Section, can cause the regulation of the focus point for sending light optional position in fluorescent dye certain limit of laser 1, certain space
It should be arranged in parallel between optical modulator 51 and xy scanning galvanometers 52.Specifically, spatial light modulator 51 can be used to receive laser
The incident light that device 1 is sent, i.e. directional light are first incident to spatial light modulator 51, in longitudinally adjust back reflection to xy scanning galvanometers 52
On, xy scanning galvanometers 52 can realize the lateral adjustments of focus position, and the directional light after certain diverging of first lens 6 also can be first
It is incident on xy scanning galvanometers 52, in lateral adjustments back reflection to spatial light modulator 51, spatial light modulator 51 carries out longitudinal direction
Regulation.
Continue to optimize above-described embodiment, femtosecond laser caused by laser 1 is directional light after the diverging of the first lens 6, is put down
The incidence angle that row light is incident on spatial light modulator 51 or xy scanning galvanometers 52 is 45 degree, then can be in spatial light modulator
Be provided with dichroscope 11 in light path between 51 and xy scanning galvanometers 52, the dichroscope 11 parallel to xy scanning galvanometers 52,
And detector 8 is located on the reflected light path of dichroscope 11.In the present embodiment, dichroscope 11 is mainly used for transmission laser device
1 sends light, at the same can reflected fluorescent light, if directional light is incident in spatial light modulator 51, through spatial light modulator 51
Dichroscope 11 is transmitted after reflection and is incident upon on xy scanning galvanometers 52, enters the second lens 7 after being reflected on xy scanning galvanometers 52,
After being focused on by the second lens 7, the focus for sending light is located on fluorescent dye, and fluoroscopic image, and fluorescence are produced on fluorescent dye
Image is incident on xy scanning galvanometers 52 for directional light after the diverging of the second lens 7, and dichroscope 11 is incident upon after reflection, and now two
Can have the function that separating laser 1 sends light and fluoroscopic image to Look mirror 11, specifically, the laser after reflection sends light
Dichroscope 11 is transmitted, and fluoroscopic image is reflexed on dichroscope 11 on optical filter 9, passes through the filtration of optical filter 9
Afterwards, fluoroscopic image is focused on detector 8 at the 3rd lens 10.
The embodiment of the present invention also provides a kind of three-D imaging method based on two-photon fluorescence, mainly employs above-mentioned
Imaging device, comprise the following steps：
Fluorescent dye is placed in the tank 2, laser 1 is worked, and light is sent caused by it into fluorescence excitation light path 3,
And through the first lens 6 diverging after be in directional light, directional light is incident to focus adjustment block 5 with 45 degree；
Focus adjustment block 5 is refined as spatial light modulator 51 and xy scanning galvanometers 52, and directional light is first incident to space
Optical modulator 51, the reflected parallel light of spatial light modulator 51, the light that sends reflected are radiated at xy scannings by dichroscope 11
On galvanometer 52, the reflection of xy scanning galvanometers 52 sends light, then focuses in fluorescent dye by the second lens 7, swashs in focal point
Send fluorescence；
Caused fluorescence scatters to the second lens 7 after being pointed into, and enters fluorescence reception light path after being dissipated by the second lens 7
4, reflected by xy scanning galvanometers 52, then optical filter 9 is reflected through by dichroscope 11, veiling glare is filtered by optical filter 9,
After the 3rd lens 10, focus in detector 8, fluoroscopic image information is obtained by detector 8, by this form, then
Fluorescence excitation light path 3 partly overlaps with fluorescence reception light path 4, specially dichroscope 11 to the light path between tank 2；
The signal being applied to by changing in spatial light modulator 51 and xy scanning galvanometers 52 changes output light and contaminated in fluorescence
The position of focus point in material, makes femtosecond laser be focused at the diverse location in dyestuff, so can be obtained by inside dyestuff
Fluorescence at diverse location, so as to draw the three-dimensional fluorescence image of needs inside dyestuff.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God any modification, equivalent substitution and improvements made etc., should be included in the scope of the protection with principle.
Claims (7)
1. a kind of three-dimensional image forming apparatus based on two-photon fluorescence, including laser and the tank that fluorescent dye can be placed, its
It is characterised by：Also include fluorescence excitation light path and fluorescence reception light path, the fluorescence excitation light path includes adjustable laser and sent out
Go out the focus adjustment block of optical focus position in fluorescent dye, the fluorescence reception light path includes that caused fluorescence can be received
Detector, and the first lens are provided with the light path between the laser and the focus adjustment block, the fluorescence connects
Receive light path and be provided with optical filter in light path between the tank and the detector, in the focus adjustment block with it is described
The second lens are provided with light path between tank, the 3rd is provided with the light path between the optical filter and the detector
Lens.
2. the three-dimensional image forming apparatus based on two-photon fluorescence as claimed in claim 1, it is characterised in that：The focal adjustments group
Part includes spatial light modulator and xy scanning galvanometers, and the spatial light modulator is indulged for adjusting exciting light in fluorescent dye
To the position of focus point, the xy scanning galvanometers are used to changing the lateral attitude of focus point, the spatial light modulator with it is described
Xy scanning galvanometers be arranged in parallel.
3. the three-dimensional image forming apparatus based on two-photon fluorescence as claimed in claim 2, it is characterised in that：Adjusted in the spatial light
Dichroscope is provided with light path between device processed and the xy scanning galvanometers, the dichroscope shakes parallel to xy scannings
Mirror, and the detector is located on the reflected light path of the dichroscope.
4. the three-dimensional image forming apparatus based on two-photon fluorescence as claimed in claim 2, it is characterised in that：The space light modulation
Device receives the incident light that the laser is sent.
5. the three-dimensional image forming apparatus based on two-photon fluorescence as claimed in claim 1, it is characterised in that：The laser is winged
Second laser, centre wavelength 800nm, pulsewidth 300fs, repetition rate 76MHz.
6. a kind of three-D imaging method based on two-photon fluorescence, it is characterised in that comprise the following steps：
In placing fluorescent dye in tank, laser sends light and enters fluorescence excitation light path, is directional light through the diverging of the first lens
It is incident to focus adjustment block；
The focus adjustment block reflection sends light, and sends light through on the fluorescent dye in the second lens entrance to tank, producing
Raw fluorescence；
Fluorescence enters fluorescence reception light path after the diverging of the second lens, and through miscellaneous in the optical filter filtering fluorescence reception light path
Astigmatism, and fluorescence enters in detector through the 3rd lens；
Change the focal position on fluorescent dye by adjusting the focus adjustment block, to obtain any bit in fluorescent dye
The three-dimensional fluorescence image put.
7. the three-D imaging method based on two-photon fluorescence as claimed in claim 6, it is characterised in that：The focal adjustments group
Part includes spatial light modulator and xy scanning galvanometers, and the directional light after diverging is incident in the spatial light modulator, through institute
State spatial light modulator to reflex on the xy scanning galvanometers, and to be incident to described second saturating after xy scanning galvanometers reflection
Mirror.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710734908.9A CN107677653A (en) | 2017-08-24 | 2017-08-24 | Three-dimensional image forming apparatus and imaging method based on two-photon fluorescence |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710734908.9A CN107677653A (en) | 2017-08-24 | 2017-08-24 | Three-dimensional image forming apparatus and imaging method based on two-photon fluorescence |
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| CN107677653A true CN107677653A (en) | 2018-02-09 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116735562A (en) * | 2023-08-15 | 2023-09-12 | 深圳湾实验室 | Three-dimensional dynamic microscopic imaging system, method and storage medium |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201167945Y (en) * | 2008-04-11 | 2008-12-24 | 中国科学院上海光学精密机械研究所 | Device for improving dual-color two-photon fluorescence imaging chromatography depth |
| CN103558193A (en) * | 2013-10-24 | 2014-02-05 | 深圳先进技术研究院 | Two-photon microscope |
| CN105548099A (en) * | 2015-12-04 | 2016-05-04 | 西北大学 | Cultural relic lossless three-dimensional imaging and component identification method based on two-photon excitation fluorescence |
| CN107037016A (en) * | 2016-02-04 | 2017-08-11 | 北京世纪桑尼科技有限公司 | A kind of confocal optical scanner |
-
2017
- 2017-08-24 CN CN201710734908.9A patent/CN107677653A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201167945Y (en) * | 2008-04-11 | 2008-12-24 | 中国科学院上海光学精密机械研究所 | Device for improving dual-color two-photon fluorescence imaging chromatography depth |
| CN103558193A (en) * | 2013-10-24 | 2014-02-05 | 深圳先进技术研究院 | Two-photon microscope |
| CN105548099A (en) * | 2015-12-04 | 2016-05-04 | 西北大学 | Cultural relic lossless three-dimensional imaging and component identification method based on two-photon excitation fluorescence |
| CN107037016A (en) * | 2016-02-04 | 2017-08-11 | 北京世纪桑尼科技有限公司 | A kind of confocal optical scanner |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116735562A (en) * | 2023-08-15 | 2023-09-12 | 深圳湾实验室 | Three-dimensional dynamic microscopic imaging system, method and storage medium |
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Application publication date: 20180209 |