CN107669746A - A kind of Chinese medical extract of resisiting influenza virus and its application - Google Patents
A kind of Chinese medical extract of resisiting influenza virus and its application Download PDFInfo
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- CN107669746A CN107669746A CN201710818064.6A CN201710818064A CN107669746A CN 107669746 A CN107669746 A CN 107669746A CN 201710818064 A CN201710818064 A CN 201710818064A CN 107669746 A CN107669746 A CN 107669746A
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- extract
- influenza virus
- chinese medical
- resisiting influenza
- water
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
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- Pharmacology & Pharmacy (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Medicines Containing Plant Substances (AREA)
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Abstract
The invention discloses a kind of Chinese medical extract of resisiting influenza virus and its application, extract is to dry hilllock to harvest leaves extract, be can be applied in the preparation of anti-influenza virus medicament.The extract of the present invention has resisiting influenza virus effect, and using south of the Five Ridges genunie medicinal materials, raw material is easy to get, and medicine extraction process is easy, is advantageous to put into big production, product quality is easy to control.
Description
Technical field
The present invention relates to field of medicaments, and in particular to a kind of Chinese herbal medicine with resisiting influenza virus effect.
Background technology
Influenza (Influenza) is the acute febrile respiratory infectious disease as caused by influenza virus, through droplet transmission, is faced
Bed typical performance is projection chilly, high fever, headache, Muscular stiffness, the weak and weary systemic toxicity profiles symptom such as weak, and respiratory symptom compared with
Gently.This disease is often in self limiting, and the course of disease is generally 3-4 days.Infant, the elderly, there are heart and lung diseases and other patients with chronic diseases
Or immunologic hypofunction person can Complicating Pneumonia In Patients, prognosis is poor.
South of the Five Ridges Chinese herbal medicine, more platymiscium herbal medicine, and mostly annual herbaceous plant, the more bitter colds of its nature and flavor, its effect
Have the function that mostly clearing heat and detoxicating, be used for catching a cold, dysentery, enteritis, hyperpyrexia polydipsia, tonsillitis, sphagitis, tracheitis,
Pertussis, catarrhal jaundice, wild gill fungus, arsenic intoxication etc..
Therefore, using Chinese Traditional Medicine resource, using modern biotechnology, with reference to the characteristics of the Chinese herbal medicine of the south of the Five Ridges, research is opened
Hair one kind has the obvious south of the Five Ridges Chinese herbal medicine of resisiting influenza virus action effect, has great importance, while also have huge city
Field potentiality to be exploited.
The content of the invention
It is an object of the invention to provide a kind of Chinese medical extract of resisiting influenza virus, have the function that clearing heat and detoxicating.
A further object of the invention is applied to above-mentioned Chinese medical extract in the preparation of anti-influenza virus medicament.
The object of the present invention is achieved like this：A kind of Chinese medical extract of resisiting influenza virus, it is characterised in that：Described
Extract is that leaves extract is harvested on hilllock.
Described extract is that dry leaves extract is harvested on hilllock.
Described extract is the water extract that dry leaf is harvested on hilllock.
Described water extract is obtained by water heating extracting.
Described water extract is paste.
Described water extract is by water heating extracting step：Take hilllock to harvest dry leaf to smash, add 8-12 times of medicinal material weight
Distilled water, extract 2 times, boil every time 2-4 hours, merge extract solution, filtering, merging filtrate, concentration, water bath method to paste
Thing, obtain extract.
Above-mentioned Chinese medical extract is applied to prepare anti-influenza virus medicament.
The anti-influenza virus medicament is using above-mentioned Chinese medical extract as active ingredient, customary adjuvant is added, according to Chinese medicine
The oral formulations that conventional formulation technique is prepared into.
Described oral formulations are tablet, capsule, granule, oral liquid or pill.
The extract of the present invention has resisiting influenza virus effect, and using south of the Five Ridges genunie medicinal materials, raw material is easy to get, medicine extraction work
Skill is easy, is advantageous to put into big production, product quality is easy to control.
Embodiment
The present invention is a kind of Chinese medical extract of resisiting influenza virus, and extract is that leaf (preferably drying leaf) extraction is harvested on hilllock
Thing, can be water extract.Water extract is paste obtained by water heating extracting.
Hilllock is harvested, and the drying leaf of (Rhodomyrtus tomentosa) is harvested on Myrtaceae Myrtus hilllock.It is sweet, puckery, property
It is flat, myogenic hemostasis, puckery intestines controlling nocturnal emission with astringent drugs, it is generally used for diarrhea, gastroenteritis, traumatism and bleeding, sore swells.
Water extract is by water heating extracting step：Take hilllock to harvest dry leaf to smash, add 8-12 times of medicinal material weight distilled water,
Extraction 2 times, boils 2-4 hours every time, merges extract solution, filtering, merging filtrate, concentration, water bath method to paste, is carried
Take thing.
Above-mentioned Chinese medical extract is applied in the preparation of anti-influenza virus medicament by the present invention.The convenience taken for patient
With the characteristic according to extract, the extract addition can be prepared various customary adjuvants required during different dosage forms, pressed by the present invention
Any oral formulations are prepared into according to Chinese medicine conventional formulation technique, such as tablet, capsule, granule, oral liquid, pill
Deng.
The preparation method of the present invention, all skills realized based on the above of the present invention will be further illustrated in the following example
Art belongs to the scope of the present invention.
Embodiment
Take hilllock to harvest dry leaf 250g, smash, add 10 times of medicinal material weight distilled water, boil 3 hours, extract 2 times, merging carries
Liquid is taken, is filtered, merging filtrate, concentration, water bath method to paste, obtains extract.
Experimental example
Example 1 is extract obtained, and resisiting influenza virus experiment is carried out as test medicine, to prove the present invention to influenza
The prevention and treatment of influenza caused by virus.
Leaf harvesting is harvested from Guangxi province Guigang Pingnan County county magistrate into the Golconda of town eight treasures (choice ingredients of certain special dishes) village three in hilllock；Influenza A virus strain A/
PR/8/34 (H1N1), mdck cell, DMEM cell culture mediums, phosphate buffer (PBS) and mtt assay agents useful for same are by south
Square pharmaceutical college of medical university give.
1. inhibitory activity when leaf water extract is used to prevent A/PR/8/34 (H1N1) is harvested on detection hilllock
(1) it is inoculated with 2X 104/ well mdck cells are to 96 hole cell culture mediums, and cell grows up to individual layer after 24 hours；
(2) remove culture medium, cell is washed 2 times with PBS.100 TCID50 of 100 times of viral dilution, i.e. final concentration.It is tested
The good various concentrations of drug dilution take 100 μ l samples and 100 μ l viral dilution liquid to be incubated 30 points in 37 DEG C respectively as sample
Clock.Sample is added to 200 μ l/well in cell with antiviral compound and is incubated 30 minutes in 37 DEG C；
(3) remove virus and sample mixture, the μ l of maintaining liquid 200 containing 0.1%TPCK are added per hole；
(4) cultivated 48 hours in 37 DEG C, the test medicine of various concentrations is detected to A/PR/8/34 (H1N1) with mtt assay
Inhibitory activity.
Data result such as table 1：
Harvest the inhibitory activity (± S, n=3) during leaf extract prevention A/PR/8/34 (H1N1) in the hilllock of table 1
| Harvest leaf extract concentration (μ g/ml) in hilllock | To A/PR/8/34 (H1N1) inhibiting rate (%) |
| 500.00 | 74.26±8.50 |
| 250.00 | 96.69±5.74 |
| 125.00 | 47.10±6.60 |
| 62.50 | 18.15±4.25 |
| 31.25 | 21.33±2.99 |
| 15.63 | 18.94±8.33 |
| 7.81 | 21.75±7.78 |
| 3.91 | 9.38±4.96 |
Experimental analysis：As a result show when leaves extract is harvested for preventing A/PR/8/34 (H1N1) in 250 μ g/ml hilllock
For inhibitory activity close to 100%, the effect of inhibitory activity when illustrating this extract to prevention A/PR/8/34 (H1N1) is obvious, carries
Show that this extract infected by influenza has preferable prevention effect.
2. inhibitory activity when leaf water extract is used to treat A/PR/8/34 (H1N1) is harvested on detection hilllock
(1) it is inoculated with 2X 104/ well mdck cells are to 96 hole cell culture mediums, and cell grows up to individual layer after 24 hours；
(2) remove culture medium, cell is washed 2 times with PBS.200 times of viral dilution, i.e., with 100 TCID50.Added per hole
100 μ l viral dilution liquid, it is incubated 1 hour in 37 DEG C；
(3) remove virus, the test medicine for having diluted concentration 200 μ l into cell are added per hole；
(4) cultivated 48 hours in 37 DEG C, the test medicine of various concentrations is detected to A/PR/8/34 (H1N1) with mtt assay
Inhibitory activity.
Data result such as table 2：
Harvest the inhibitory activity (± S, n=3) during leaf extract treatment A/PR/8/34 (H1N1) in the hilllock of table 2
| Harvest leaf extract concentration (μ g/ml) in hilllock | To A/PR/8/34 (H1N1) inhibiting rate (%) |
| 500.00 | -2.14±5.84 |
| 250.00 | 8.55±5.26 |
| 125.00 | 46.16±6.72 |
| 62.50 | 68.93±1.89 |
| 31.25 | 47.59±7.41 |
| 15.63 | 31.51±6.01 |
| 7.81 | 22.62±3.93 |
| 3.91 | 23.31±5.12 |
Experimental analysis：As a result show when leaves extract is harvested for treating A/PR/8/34 (H1N1) in 62.5 μ g/ml hilllock
Inhibitory activity is more than 70%, and the effect of inhibitory activity when illustrating this extract to treatment A/PR/8/34 (H1N1) is obvious, prompts
This extract infected by influenza has preferable therapeutic action.
Claims (9)
- A kind of 1. Chinese medical extract of resisiting influenza virus, it is characterised in that：Extract is that leaves extract is harvested on hilllock.
- 2. the Chinese medical extract of resisiting influenza virus according to claim 1, it is characterised in that：Extract is that dried leaf is harvested on hilllock Seed extract.
- 3. the Chinese medical extract of resisiting influenza virus according to claim 1 or 2, it is characterised in that：Described extract is Water extract.
- 4. the Chinese medical extract of resisiting influenza virus according to claim 3, it is characterised in that：Described water extract is by water Obtained by heating extraction.
- 5. the Chinese medical extract of resisiting influenza virus according to claim 3, it is characterised in that：Described water extract is cream Shape thing.
- 6. the Chinese medical extract of resisiting influenza virus according to claim 3, it is characterised in that：Described water extract is by water Heating extraction step is：Take hilllock to harvest dry leaf to smash, add 8-12 times of medicinal material weight distilled water, extract 2 times, boil 2-4 every time Hour, merge extract solution, filtering, merging filtrate, concentration, water bath method to paste, obtain extract.
- 7. it is applied to according to Chinese medical extract described in claim 1-6 in the preparation of anti-influenza virus medicament.
- 8. application according to claim 7, it is characterised in that：The anti-influenza virus medicament is with claim 1-6 institutes It is active ingredient to state Chinese medical extract, adds customary adjuvant, the oral formulations being prepared into according to Chinese medicine conventional formulation technique.
- 9. application according to claim 8, it is characterised in that：Described oral formulations be tablet, capsule, granule, Oral liquid or pill.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710818064.6A CN107669746B (en) | 2017-09-12 | 2017-09-12 | Anti-influenza virus traditional Chinese medicine extract and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710818064.6A CN107669746B (en) | 2017-09-12 | 2017-09-12 | Anti-influenza virus traditional Chinese medicine extract and application thereof |
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| Publication Number | Publication Date |
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| CN107669746A true CN107669746A (en) | 2018-02-09 |
| CN107669746B CN107669746B (en) | 2021-04-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710818064.6A Active CN107669746B (en) | 2017-09-12 | 2017-09-12 | Anti-influenza virus traditional Chinese medicine extract and application thereof |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010064061A1 (en) * | 2008-03-20 | 2010-06-10 | Zhou James Liu | Pharmaceutical compositions and methods of sinonasal delivering the same |
-
2017
- 2017-09-12 CN CN201710818064.6A patent/CN107669746B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010064061A1 (en) * | 2008-03-20 | 2010-06-10 | Zhou James Liu | Pharmaceutical compositions and methods of sinonasal delivering the same |
Non-Patent Citations (1)
| Title |
|---|
| 谭树辉等: "《中草药野外识别手册（一）》", 31 January 2004 * |
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| CN107669746B (en) | 2021-04-27 |
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