CN107667957A - A kind of fish resting metabolic measure device of variable-volume - Google Patents

A kind of fish resting metabolic measure device of variable-volume Download PDF

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Publication number
CN107667957A
CN107667957A CN201711091357.5A CN201711091357A CN107667957A CN 107667957 A CN107667957 A CN 107667957A CN 201711091357 A CN201711091357 A CN 201711091357A CN 107667957 A CN107667957 A CN 107667957A
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CN
China
Prior art keywords
water
measure
upper tank
pipe
volume
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201711091357.5A
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Chinese (zh)
Inventor
付成
付世建
曹振东
彭姜岚
王蕾
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Chongqing Normal University
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Chongqing Normal University
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Filing date
Publication date
Application filed by Chongqing Normal University filed Critical Chongqing Normal University
Priority to CN201711091357.5A priority Critical patent/CN107667957A/en
Publication of CN107667957A publication Critical patent/CN107667957A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Zoology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention discloses a kind of fish resting metabolic of variable-volume to determine device, including the measure pipe in upper tank, measure pipe includes the cylinder of a rear end perforate, the rear portion of cylinder has movable sealing plug, one end connection movable sealing plug of stainless steel tube, water is discharged into from barrel front end by dissolved oxygen measurement bottle followed by water pump, water pump;Mesh sheet and porous barrier are disposed with the rear portion of cylinder;The limited post to advance for barrier activity sealing-plug is provided with cylinder inboard wall, the next tank is connected by outlet pipe in the front portion of limited post;Water is discharged into upper tank by the water pump in the next tank by rising main.In the present invention, measure pipe(Respiratory chamber)Volume can accurate adjustment, only need a set of respiratory chamber with regard to the measure of different size test fish Resting Metabolism can be met.

Description

A kind of fish resting metabolic measure device of variable-volume
Technical field
The present invention relates to a kind of fish experimental provision.
Background technology
Resting Metabolism refers to metabolisable energy consumption of the animal under quiet, fasting state, can be anti-to a certain extent Reflect the physiological status of animal thus enjoy researcher to pay close attention to.Experimental rig currently used for fish Resting Metabolism measure mainly has Continuous-flow type and batch (-type)(Closed)Two kinds, the problem of the test fish size that two kinds of experimental rig can run into:Measure Respiratory chamber is excessive, and the decline of dissolved oxygen can be very slow, influences measurement accuracy.Further for activity space for less test fish It is very big, the autogenic movement more compared with small test fish may be caused, influence the measure of Resting Metabolism;And determine respiratory chamber mistake Small, dissolved oxygen declines too fast, the low-oxygen environment being likely to result in respiratory chamber, test fish is caused to coerce, in addition too small breathing Room can not also determine larger test fish.Currently used solution is to of different sizes using the different respiratory chamber of volume Test fish is measured, but also brings along the problem of new:Respiratory chamber is of different sizes, activity space of the test fish in respiratory chamber Difference, the spontaneous activity of test fish can show larger difference, and then influence measurement accuracy;In addition this solution is used The respiratory chamber of several fixed volumes can only be made, and test fish body weight is often widely different, is still difficult to meet the needs of experiment, And frequently replacing respiratory chamber is operationally relatively complicated, there is also the problem of waste for the making of a large amount of respiratory chambers.
The content of the invention
It is an object of the invention to provide a kind of fish resting metabolic of variable-volume to determine device.
To achieve these goals, using following technical scheme:
A kind of fish resting metabolic measure device of variable-volume, including the measure pipe in upper tank, it is characterised in that: Measure pipe includes the cylinder of a rear end perforate, and the rear portion of cylinder has movable sealing plug, one end connection activity of stainless steel tube Sealing-plug, water is discharged into from barrel front end by dissolved oxygen measurement bottle followed by water pump, water pump;It is disposed with the rear portion of cylinder Mesh sheet and porous barrier;The limited post to advance for barrier activity sealing-plug is provided with cylinder inboard wall, in the front portion of limited post The next tank is connected by outlet pipe;Water is discharged into upper tank by the water pump in the next tank by rising main.
The water of measurement bottle is entered by water inlet, is flowed out after dissolved oxygen probe from delivery port, and sealing ring fixes closure In on dissolved oxygen probe, there is the screw thread of matching on the inside of closure with measurement bottle outside.
Up-hill line bending is stretched into upper tank water, and the side in the middle part of upper tank is provided with dividing plate.
Upper tank is provided with upper tank retaining wall close to the side of movable sealing plug, and unnecessary water is from upper tank retaining wall Diffuse out, enter the next tank by descending water pipe.
In the present invention, measure pipe(Respiratory chamber)Volume can accurate adjustment, only need a set of respiratory chamber with regard to different size can be met The measure of test fish Resting Metabolism;During breathing building volume regulation, the activity space of test fish does not change, and avoids Respiratory chamber stereomutation impacts to test fish autogenic movement;Pump working is avoided to cause by the installation of dividing plate, mesh sheet Jet and attraction interfere to test fish;Changing lower of aqueous mode and need to opening magnetic valve just can complete, in whole respiratory chamber Need a small water pump just to meet operation, reduce heat production, while the installation of U-shaped stainless steel tube is also beneficial to avoid temperature liter in pipe Height, metabolic determination is impacted.
Brief description of the drawings
Fig. 1 is the structural representation of specific embodiment;
Fig. 2 is the concrete structure schematic diagram that dissolved oxygen measures bottle.
In figure, 1 is rising main;2 be solenoid valve controller;3 be magnetic valve;4 be oral siphon;5 be circulating water pipe;6 are Dissolved oxygen measures bottle;7 be dissolved oxygen probe;8 be measure pipe;9 be limited post;10 be movable sealing plug;11 be dissolved oxygen meter;12 be stainless Steel pipe;13 be upper tank retaining wall;14 be upper tank;15 be water valve;16 be dividing plate;17 be water pump;18 be support;19 be to fill Oxygen pump;20 be mesh sheet;21 be porous barrier;22 be check valve;23 be outlet pipe;24 be descending water pipe;25 be water pump;26 be to fill Gas sand head;27 be the next tank;28 be sealing ring;29 be measurement bottle closure;30 be measurement bottle water inlet;31 go out for measurement bottle The mouth of a river.
Embodiment
The present invention will be further described with specific embodiment below in conjunction with the accompanying drawings.
As depicted in figs. 1 and 2, the fish resting metabolic measure device of a kind of variable-volume, including in upper tank Measure pipe, measure pipe includes the cylinder of a rear end perforate, and the rear portion of cylinder has movable sealing plug, one end of stainless steel tube Movable sealing plug is connected, is discharged into water from barrel front end by dissolved oxygen measurement bottle followed by water pump, water pump;The rear portion of cylinder according to It is secondary to be provided with mesh sheet and porous barrier;The limited post to advance for barrier activity sealing-plug is provided with cylinder inboard wall, spacing The front portion of post connects the next tank by outlet pipe;Water is discharged into upper tank by the water pump in the next tank by rising main In.
Pipe 8 is wherein determined as cylinder, manage in the symmetrical porous barrier 21 of installation and mesh sheet 20, mesh sheet 20 in inner side, every Plate 21 is in outside;Left side dividing plate and mesh sheet are detachable, facilitate being placed and taken out for test fish;Test fish is located at two nets during experiment Between piece so that no matter determine breathing building volume it is much, the activity space of test fish keeps constant, can eliminate change respiratory chamber Influence of the volume to the spontaneous activity of test fish;Porous barrier 21 may be such that the water into test fish activity space is more uniform, And the interface that measure pipe 8 will not be connected in water pump forms jet or suction, and test fish is interfered;Dividing plate 21 and mesh sheet 20 Between have interval, avoid current direct contact test fish after the hole outflow on dividing plate 21, reduce the interference to test fish;
Movable sealing plug 10 can enter line slip along U-shaped stainless steel tube 12 in measure pipe 8, to change the actual volume for determining pipe 8, The measure pipe external labeling of pipe 8 has the volume markings calculated, and fine-tuning for the volume of pipe 8 is determined during convenient experiment;
The top connecting pipe 4 of pipe 8 is determined, cut-offfing for pipeline 4 is controlled by magnetic valve 3, and the front end of pipeline 4 is reached deep down into water, is avoided In bubble suction respiratory chamber;The lower section connecting pipe 23 of pipe 8 is determined, check valve 22 is installed on pipeline 23;Determine the lower section water outlet of pipe 8 Limited post is installed on the right side of mouthful, prevents movable sealing plug 10 is excessively mobile to the left from blocking lower section delivery port;
The inside of the connection measure of U-shaped stainless steel tube 12 pipe 8 and measurement bottle 6;In-pipe flow direction and the current side of outside water bath To on the contrary, and be beneficial to radiating for stainless steel, avoid caused by pump working temperature in pipe from raising and influence metabolic determination;
The measurement water of bottle 6 is entered by water inlet 30, is flowed out after dissolved oxygen probe 7 from delivery port 31.Sealing ring 28 is by closure 29 It is fixed on dissolved oxygen probe 7, but is freely rotatable;The inner side of closure 29 has the screw thread of matching with the outside of measurement bottle 6, can rotate Closure 29 makes closure 29 be connected with measurement bottle 6, while crush seal circle 28, reaches the effect of sealing;
Determine the water inlet pipe 4 and lower section delivery port of pipe 8(At check valve 22)It is poor with liquid level, when opening magnetic valve 3, external water Automatically measure pipe 8 can be flowed into through pipeline 4, and is flowed out from pipeline 23;
Water valve 15 is installed, to adjust the water into upper tank 14 on water supply pipeline 1;In the bending deeply of water supply pipeline 1 In the tank water of position, and right side is provided with dividing plate 16, prevents from being inhaled into pipeline 4 by bubble, into measure pipe 8;
Sewer pipe 24 gos deep into the next tank 27, prevents current from falling directly into the water surface, produces very big noise, test fish is made Into influence.
In experimentation, measure can be divided and change water both of which by carrying out experiment using measure pipe main body, in measure mould Under formula, magnetic valve 3 is controlled to close by controller 2, water pump 17 extracts water out from measure pipe 8, by Stainless Steel U pipe 12 and surveys Measuring bottle 6, return in measure pipe 8, realize that water body exchanges and dissolved oxygen determines;Change under aqueous mode, magnetic valve 3 is opened, due to the He of pipeline 4 It is poor to there is liquid level between check valve 22, external water enters measure pipe 8 by pipeline 4, while determines the water in pipe 8 from check valve Flowed out in 22, into the next tank 27.
In circulator bath environment:Water is pumped into upper tank by water pump 25 from the next tank 27 through pipeline 1, then is covered The right side inwall 13 of upper tank 14, returned to through pipeline 24 in the next tank 27.

Claims (4)

1. a kind of fish resting metabolic measure device of variable-volume, including the measure pipe in upper tank, its feature exist In:Measure pipe includes the cylinder of a rear end perforate, and the rear portion of cylinder has movable sealing plug, and one end connection of stainless steel tube is lived Dynamic sealing plug, water is discharged into from barrel front end by dissolved oxygen measurement bottle followed by water pump, water pump;Set gradually at the rear portion of cylinder There are mesh sheet and porous barrier;The limited post to advance for barrier activity sealing-plug is provided with cylinder inboard wall, before limited post Portion connects the next tank by outlet pipe;Water is discharged into upper tank by the water pump in the next tank by rising main.
2. the fish resting metabolic measure device of variable-volume as claimed in claim 1, it is characterised in that:Measure bottle water by Water inlet enters, and is flowed out after dissolved oxygen probe from delivery port, closure is fixed on dissolved oxygen probe by sealing ring, in closure Side and the screw thread for having matching on the outside of measurement bottle.
3. the fish resting metabolic measure device of variable-volume as claimed in claim 1, it is characterised in that:Up-hill line is bent Stretch into upper tank water, and the side in the middle part of upper tank is provided with dividing plate.
4. the fish resting metabolic measure device of variable-volume as claimed in claim 1, it is characterised in that:Upper tank is close The side of movable sealing plug is provided with upper tank retaining wall, and unnecessary water is diffused out from upper tank retaining wall, entered by descending water pipe Enter the next tank.
CN201711091357.5A 2017-11-08 2017-11-08 A kind of fish resting metabolic measure device of variable-volume Pending CN107667957A (en)

Priority Applications (1)

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CN201711091357.5A CN107667957A (en) 2017-11-08 2017-11-08 A kind of fish resting metabolic measure device of variable-volume

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Application Number Priority Date Filing Date Title
CN201711091357.5A CN107667957A (en) 2017-11-08 2017-11-08 A kind of fish resting metabolic measure device of variable-volume

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111493002A (en) * 2020-03-19 2020-08-07 菏泽学院 Device for measuring activity and metabolic rate of pelteobagrus vachelli under low oxygen

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SU1169575A1 (en) * 1984-01-18 1985-07-30 Всесоюзный Научно-Исследовательский Институт По Охране Вод Method and apparatus for consumption of oxygen by water organisms
WO1995014925A1 (en) * 1993-11-23 1995-06-01 Nancie - Centre International De L'eau Method and apparatus for monitoring aqueous media using electrolocating aquatic animals
CN2850274Y (en) * 2005-12-12 2006-12-27 重庆师范大学 Respirator water supply device capable of separating survival water with bathing water
CN2850270Y (en) * 2005-12-12 2006-12-27 重庆师范大学 Pressure differential type fish swimming capacity testing device
CN102687691A (en) * 2012-05-02 2012-09-26 中国水产科学研究院东海水产研究所 Apparatus for testing trans-epithelium potential of fish body
CN103461232A (en) * 2013-08-08 2013-12-25 中国水产科学研究院长江水产研究所 Testing device and testing method for testing environmental preference behavior of fish
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SU1169575A1 (en) * 1984-01-18 1985-07-30 Всесоюзный Научно-Исследовательский Институт По Охране Вод Method and apparatus for consumption of oxygen by water organisms
WO1995014925A1 (en) * 1993-11-23 1995-06-01 Nancie - Centre International De L'eau Method and apparatus for monitoring aqueous media using electrolocating aquatic animals
CN2850274Y (en) * 2005-12-12 2006-12-27 重庆师范大学 Respirator water supply device capable of separating survival water with bathing water
CN2850270Y (en) * 2005-12-12 2006-12-27 重庆师范大学 Pressure differential type fish swimming capacity testing device
CN102687691A (en) * 2012-05-02 2012-09-26 中国水产科学研究院东海水产研究所 Apparatus for testing trans-epithelium potential of fish body
CN103461232A (en) * 2013-08-08 2013-12-25 中国水产科学研究院长江水产研究所 Testing device and testing method for testing environmental preference behavior of fish
CN103975881A (en) * 2014-05-12 2014-08-13 中国水产科学研究院黑龙江水产研究所 Oxygen consumption rate and choke point measuring device of fishes
CN104904632A (en) * 2015-05-20 2015-09-16 重庆师范大学 Test device for determining fish critical oxygen pressure

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111493002A (en) * 2020-03-19 2020-08-07 菏泽学院 Device for measuring activity and metabolic rate of pelteobagrus vachelli under low oxygen

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Application publication date: 20180209