CN107660463A - A kind of giantreed tissue culture tiller fast breeding culture medium - Google Patents
A kind of giantreed tissue culture tiller fast breeding culture medium Download PDFInfo
- Publication number
- CN107660463A CN107660463A CN201610597056.9A CN201610597056A CN107660463A CN 107660463 A CN107660463 A CN 107660463A CN 201610597056 A CN201610597056 A CN 201610597056A CN 107660463 A CN107660463 A CN 107660463A
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- culture medium
- tiller
- giantreed
- seedling
- tissue
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- 235000014676 Phragmites communis Nutrition 0.000 title claims abstract description 35
- 239000001963 growth medium Substances 0.000 title claims abstract description 35
- 241001494508 Arundo donax Species 0.000 title claims abstract description 33
- 238000009395 breeding Methods 0.000 title claims abstract description 8
- 230000001488 breeding effect Effects 0.000 title claims abstract description 8
- 230000006698 induction Effects 0.000 claims abstract description 6
- 230000001360 synchronised effect Effects 0.000 claims abstract 2
- 229920001817 Agar Polymers 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- 229930006000 Sucrose Natural products 0.000 claims description 4
- 239000005720 sucrose Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 abstract description 9
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 2
- 239000005648 plant growth regulator Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 11
- 239000004576 sand Substances 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 241001529246 Platymiscium Species 0.000 description 2
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- 235000007201 Saccharum officinarum Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000004459 forage Substances 0.000 description 2
- 230000001976 improved effect Effects 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920002955 Art silk Polymers 0.000 description 1
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- 241000283690 Bos taurus Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
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- 206010037660 Pyrexia Diseases 0.000 description 1
- 244000007853 Sarothamnus scoparius Species 0.000 description 1
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
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- 210000002700 urine Anatomy 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to a kind of giantreed tissue-culturing rapid propagation culture medium, basic MS is combined into a kind of culture medium prescription added with plant growth regulator, use the tissue-culturing rapid propagation culture medium of the present invention, excellent strain axillary bud stem section is explant, whole sterile culture process is simple and easy, it might even be possible to is simplified to a kind of culture medium (once-seedling forming and tiller fast breeding culture medium).Squamous subculture, stipes root induction seedling, the synchronous progress of root section tiller propagation, shorten giantreed tissue-cultured seedling growing-seedling period, are advantageous to industrialization production in tiller culture medium.
Description
Technical field
The present invention relates to a kind of tissue-culturing rapid propagation culture medium of plant, more particularly to giantreed tissue culture tiller fast breeding culture medium.
Background technology
Giantreed platymiscium is the perennial very tall and big perennial root draft of water, shaped like reed.Subterranean stem cripetura, sturdy, multi-branched, leaf
The wide lanceolar of piece, panicle basidixed, spike are in broom shape, and the 9-12 months are flowering fruit bearing stage.For main waterside view plant.Giantreed is
A kind of very effective corn substitute, for biomass, giantreed is a kind of annual plant for having huge yield potential,
Its productivity can keep 10-15, and adapt to marginal soil.In addition, giantreed also has one very big advantage, i.e., make in production
The fertility of soil will not be taken away while thing.Stalk is the reed in tubulation musical instrument.Fasciated fiber is grown, and length and width ratio is big, and cellulose contains
Amount is high, is the raw material of the high-quality paper pulp of system and artificial silk.The thick protein of young browse is the good greenfeed of livestock up to 12%.
Root-like stock (giantreed)：Hardship, tremble with fear.Clearing away heat and promoting diuresis.Gone mad for pyreticosis, consumptive disease hectic fever due to yin, stranguria, urine leaching pain is unfavorable, acute toothache.
In general giantreed is distributed in the torrid zone, the ground such as subtropical zone, Chinese East China, south China and southwest, and happiness is warm, and happiness water is wet, and cold resistance is not strong,
The north needs protecting field to survive the winter.But research in recent years and introducing is found, part giantreed can in Winter in North China, it is drought-resistant and
It is barren, well developed root system, sand grassland can be rebuild within a short period of time, tackling quicksand, biological production is high, while nutritive value
Height, palatability is good, can be as the good forage of cattle and sheep, and Its Eco-economic Benefit is notable, to the fragmentary plant on sand land of China humid region
Significant with Efficient Development, the improvement to other sand grounds also has experiment prospect.
" oasis No.1 " giantreed that Lin Zhanxiong such as " father of world's Juncao " names, normally survives the winter biennial green in Tibet
The average height 135cm of continent 1, the mean tillering number 15,8500 kilograms of per mu yield fresh weight；The dense prosperity of root system, depth more than 150cm,
Sand ground is completely fixed, and effect is clearly.The giantreed that national office of poverty alleviation promotes makees the giantreed of forage grass, Baiyang Lake, Weishan Lake
Giantreed etc. can be in Winter in North China.So using giantreed afforestation fix the sand, wetland transformation, ornamental plantation band, production herbage, plantation eat
Bacterium, improvement sewage etc. have extensive purposes, and especially the ecological management of northern China is had far-reaching significance.
However, the seed-setting rate of giantreed is low, kind section reproductive survival rate is extremely low, limits the popularization and application of its large area.
Usual way is to divide root or 2-3 stipes cuttage and quick-propagation to carry out using it.With 2-4 stem segment cuttages, survival rate is unstable,
Between 40%-80%, the survival rate of simple node plant, only 20% or so.In consideration of it, the tissue-culturing rapid propagation of giantreed be it is a kind of very
Good breeding and seedling mode, conventional giantreed tissue culture method are divided to inducing clumping bud propagation and two steps of rooting induction, its
Stipes is once for " oasis No.1 " Juncao (a kind of giantreed) described in Chinese patent application CN105248280A submitted with us
Seedling group culturation rapid propagating technology is suitable；However, in order to further increase fast numerous coefficient, save cost and shorten tissue-cultured seedling nursery week
Phase, beneficial to industrialization production.Our further scientific researches on the basis of aforementioned patent applications, find by the tiller on culture medium
It is fast numerous, fast numerous speed and quality can be significantly improved, the giantreed seedling with higher plantation survival rate is obtained, can solve the problem that current kind
The problem of seedling is provided in the plant phase for vast farmland.
The content of the invention
The purpose of the present invention is overcome the deficiencies in the prior art, there is provided a kind of tillering propagation culture of giantreed tissue-culturing rapid propagation
Base, tiller propagation and stem section propagation once-seedling forming are synchronously carried out on the tiller culture medium, further shorten growing-seedling period, favorably
In industrialization production.
Core technology scheme of the present invention is：During Initial culture, compared to existing culture medium, it can adopt
With the culture medium for promoting inducing clumping bud and point evil simultaneously, micropropagation efficiency and quality are improved so as to reach, obtains high-survival rate
The purpose of seedling.
On tiller culture medium during tissue culture giantreed, the culture medium is in addition to it can make stipes once-seedling forming, moreover it is possible to make root section (root
Portion) constantly tiller emergence, stipes is cut off and the segmentation tiller of root section is carried out simultaneously, further improves breeding coefficient.
The culture medium of the giantreed tissue culture and rapid propagation method of the present invention forms：
A. tiller culture medium (1/2MS or MS+0.1-0.5mg/LNAA+5mg/L IBA+15g sucrose+7g agar), once
Observe that axillary bud is sprouted on seedling culture medium after 3-4 days, leaf bud base portion sprouts root system within 6-20 days, you can forms complete small plant
Strain, now this plant is connected in thicker stem section, i.e., on former explant.
B. Multiple Buds culture medium (1/2MS or MS+1-4mg/L 6-BA+0.1mg/LNAA+5mg/L IBA+15g sugarcanes are induced
Sugar+7g agar), 10 days or so presidents go out the Multiple Buds of no root, can grow within 20-30 days or so 3-15 left-right parts band root
Multiple Buds or seedling.
Tiller culture medium and induction Multiple Buds culture medium, the tissue-culturing rapid propagation of giantreed can be simplified on tiller culture medium
On a kind of culture medium, root, tiller, growth is lured once to complete, stipes once-seedling forming and tiller are fast numerous to be completed simultaneously.
The fast numerous and root section tiller of stipes segment is combined, their combination improves the fast numerous fast numerous coefficient of giantreed and effect
Rate.
The culture medium can apply to the detoxification, fast numerous of all giantreed platymisciums according to tissue-culturing rapid propagation specific steps.
Brief description of the drawings
Its example embodiment is described in detail by referring to accompanying drawing, above and other target of the invention, feature and advantage will
Become more fully apparent.
Fig. 1 show stipes once-seedling forming
Fig. 2 show the tiller of root section, growth
Fig. 3 show Multiple Buds take root, tiller, growth
Fig. 4 show substantial amounts of root section tiller propagation system
Fig. 5 show substantial amounts of Multiple Buds take root tiller propagation system
Fig. 6 show a large amount of giantreed seedlings of transplant survival
Embodiment
Explant Initial culture：By the explant disinfected (stipes) and then oblique cutting or vertically it is inserted in be equipped with and prepares
Culture medium vial in cultivate, two kinds of different culture mediums that Initial culture can use are respectively：
A. tiller culture medium (1/2MS or MS+0.1-0.5mg/LNAA+5mg/L IBA+15g sucrose+7g agar), inoculation
Observe that axillary bud is sprouted after by 3-4 days thereon, leaf bud base portion sprouts root system within 6-20 days, you can complete plantlet is formed, this
When this plant be connected in thicker stem section, i.e., on former explant.
B. Multiple Buds culture medium (1/2MS or MS+1-4mg/L 6-BA+0.1mg/LNAA+5mg/L IBA+15g sugarcanes are induced
Sugar+7g agar), 10 days or so presidents go out the Multiple Buds of no root, can grow within 20-30 days or so 3-15 left-right parts band root
Multiple Buds or seedling.
The Multiplying culture of seedling：
A. on tiller culture medium Initial culture the plant for having 2-5 stipes, on super-clean bench peel off and segment, every section one
Individual section position, it is numerous soon to be seeded in progress once-seedling forming on tiller culture medium；Root will also stay a section position inoculation, be seeded in tiller training
Support on base, realize the stem section propagation of next round and tillering propagation while carry out.
B. the Multiple Buds or seedling of primary induction Multiple Buds and tiller culture medium upper part band root, the plant segment for having stipes connect
Kind is numerous soon to progress once-seedling forming on tiller culture medium；Root is inoculated into realization on tiller culture medium and lures root, tiller and stem section life
It is long.
It is an advantage of the current invention that general giantreed Multiple Buds system, first induces Multiple Buds, Multiple Buds certain altitude Gao Shizai
Separately induce root.We are realizing giantreed stipes soon on the basis of numerous once-seedling forming, stem section of the present invention cut by segment it is fast numerous and
Root tiller is combined, and substantially increases breeding coefficient.Tissue-culturing rapid propagation tiller technology do not report in conventional tissue-culturing rapid propagation,
It is different from the system that first induction Multiple Buds lure root again, similar to the tiller of crop field giantreed or other crops (such as wheat, rice).
Claims (1)
1. a kind of giantreed tissue culture tiller fast breeding culture medium, it is characterised in that its composition is：
1/2MS or MS+ (0.1-0.5) mg/LNAA+5mg/L IBA+15g sucrose+7g agar, or MS+ (0.1-0.5) mg/LNAA
+ 5mg/L IBA+15g sucrose+7g agar；The squamous subculture in tiller culture medium, stipes root induction seedling, the tiller of root section increase
Grow synchronous progress.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610597056.9A CN107660463A (en) | 2016-07-27 | 2016-07-27 | A kind of giantreed tissue culture tiller fast breeding culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610597056.9A CN107660463A (en) | 2016-07-27 | 2016-07-27 | A kind of giantreed tissue culture tiller fast breeding culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107660463A true CN107660463A (en) | 2018-02-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610597056.9A Pending CN107660463A (en) | 2016-07-27 | 2016-07-27 | A kind of giantreed tissue culture tiller fast breeding culture medium |
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| Country | Link |
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| CN (1) | CN107660463A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112293260A (en) * | 2020-11-24 | 2021-02-02 | 福建农林大学 | Method for obtaining tissue culture seedlings of reed in Africa |
| EP3903577A4 (en) * | 2018-12-28 | 2022-01-26 | Wuhan Rundo Biotechnology Co. Ltd | Method for growing arundo donax plant |
| CN114208679A (en) * | 2022-01-19 | 2022-03-22 | 内蒙古农业大学 | Tissue culture rapid propagation method of oasis 1 juncao |
| CN116267602A (en) * | 2022-12-20 | 2023-06-23 | 郑州大学 | Tissue culture method for rapidly culturing reed leaf and reed seedling |
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|---|---|---|---|---|
| CN102919127A (en) * | 2012-11-12 | 2013-02-13 | 湖州师范学院 | Method for building bamboo reed tissue culture system |
| CN103229720A (en) * | 2013-04-26 | 2013-08-07 | 湖州师范学院 | Method for regenerating plant from arundo donax linn callus |
| CN103749302A (en) * | 2014-01-15 | 2014-04-30 | 江苏沿海地区农业科学研究所 | Induced acclimation and cultivation method for salt-tolerant bamboo reed seedlings |
| CN105248280A (en) * | 2015-10-30 | 2016-01-20 | 王晓翔 | One-step seedling culturing tissue culture and rapid propagation method of pennisetum hydridum |
-
2016
- 2016-07-27 CN CN201610597056.9A patent/CN107660463A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919127A (en) * | 2012-11-12 | 2013-02-13 | 湖州师范学院 | Method for building bamboo reed tissue culture system |
| CN103229720A (en) * | 2013-04-26 | 2013-08-07 | 湖州师范学院 | Method for regenerating plant from arundo donax linn callus |
| CN103749302A (en) * | 2014-01-15 | 2014-04-30 | 江苏沿海地区农业科学研究所 | Induced acclimation and cultivation method for salt-tolerant bamboo reed seedlings |
| CN105248280A (en) * | 2015-10-30 | 2016-01-20 | 王晓翔 | One-step seedling culturing tissue culture and rapid propagation method of pennisetum hydridum |
Non-Patent Citations (4)
| Title |
|---|
| 冉隆贤等: "芦竹组织培养技术研究", 《中南林学院学报》 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3903577A4 (en) * | 2018-12-28 | 2022-01-26 | Wuhan Rundo Biotechnology Co. Ltd | Method for growing arundo donax plant |
| CN112293260A (en) * | 2020-11-24 | 2021-02-02 | 福建农林大学 | Method for obtaining tissue culture seedlings of reed in Africa |
| CN114208679A (en) * | 2022-01-19 | 2022-03-22 | 内蒙古农业大学 | Tissue culture rapid propagation method of oasis 1 juncao |
| CN116267602A (en) * | 2022-12-20 | 2023-06-23 | 郑州大学 | Tissue culture method for rapidly culturing reed leaf and reed seedling |
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Application publication date: 20180206 |