CN107652970B - Preparation method and application of carbon-based nano hybrid material - Google Patents
Preparation method and application of carbon-based nano hybrid material Download PDFInfo
- Publication number
- CN107652970B CN107652970B CN201710805819.9A CN201710805819A CN107652970B CN 107652970 B CN107652970 B CN 107652970B CN 201710805819 A CN201710805819 A CN 201710805819A CN 107652970 B CN107652970 B CN 107652970B
- Authority
- CN
- China
- Prior art keywords
- carbon
- solution
- hybrid material
- nitrogen
- lpqds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/65—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing carbon
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
- B01J31/16—Catalysts comprising hydrides, coordination complexes or organic compounds containing coordination complexes
- B01J31/18—Catalysts comprising hydrides, coordination complexes or organic compounds containing coordination complexes containing nitrogen, phosphorus, arsenic or antimony as complexing atoms, e.g. in pyridine ligands, or in resonance therewith, e.g. in isocyanide ligands C=N-R or as complexed central atoms
- B01J31/1805—Catalysts comprising hydrides, coordination complexes or organic compounds containing coordination complexes containing nitrogen, phosphorus, arsenic or antimony as complexing atoms, e.g. in pyridine ligands, or in resonance therewith, e.g. in isocyanide ligands C=N-R or as complexed central atoms the ligands containing nitrogen
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/02—Use of particular materials as binders, particle coatings or suspension media therefor
- C09K11/025—Use of particular materials as binders, particle coatings or suspension media therefor non-luminescent particle coatings or suspension media
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2531/00—Additional information regarding catalytic systems classified in B01J31/00
- B01J2531/10—Complexes comprising metals of Group I (IA or IB) as the central metal
- B01J2531/16—Copper
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Materials Engineering (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Optics & Photonics (AREA)
- General Physics & Mathematics (AREA)
- Inorganic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Pathology (AREA)
- Composite Materials (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Health & Medical Sciences (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a preparation method of a carbon-based nano hybrid material, which comprises the following steps: (1) preparing a nitrogen-doped carbon quantum dot solution by using citric acid as a carbon source and urea as a nitrogen source; (2) mixing the nitrogen-doped carbon quantum dot solution with the phenylalanine amide ligand solution, and respectively adding a carboxyl activating agent 1-ethyl- (3-dimethylaminopropyl) carbonyl diimine hydrochloride, 1-hydroxybenzotriazole and 4-dimethylaminopyridine to prepare an LPQDs solution; (3) adding Cu (NO) to LPQDs solution3)2And (3) reacting the solution to obtain the Cu (II) -LPQDs nano hybrid material. The method adopts non-traditional high-temperature high-pressure hydrothermal reaction to prepare NCQDs, avoids using high-toxicity chemical reagents, and improves the fluorescence property of carbon quantum dots by doping nitrogen elements; compared with a noble metal enzyme catalyst, the invention adopts non-noble metal Cu as a catalytic active center, and the catalytic performance is superior to that of the noble metal enzyme catalyst; cu (II) -LPQDs carbon-based nano hybrid material can be used for H in human cervical carcinoma cells2O2Detection of (3).
Description
Technical Field
The invention belongs to the technical field of functional materials, and particularly relates to a preparation method and application of a carbon-based nano hybrid material.
Background
Zero-dimensional nanostructures have been the subject of intense research by researchers due to their interesting properties and unique applications. Carbon Quantum Dots (CQDs) are a novel carbon nanomaterial with a quasi-spherical structure and stable fluorescence performance, and the size of the carbon nanomaterial is less than 10 nm. Compared with the traditional semiconductor quantum dots and organic dyes, CQDs have the characteristics of low toxicity, good dispersibility, strong chemical inertness, simple functionalization, good photobleaching resistance, excellent biocompatibility and the like. Recently, the abundant physicochemical properties of nitrogen-doped nanocarbon materials (graphene, carbon nanotubes, carbon nanofibers, etc.) are receiving attention from researchers due to their ability to induce charge delocalization and adjust the overall function of carbon materials. Copper is the third most abundant soft transition metal ion in the human body, and is also an important mineral nutrient element of mammalian cells, compared to iron and zinc, and at the same time, it serves as a cofactor for many enzymes, in other words, it plays a very important role in various biological processes. Metalloenzymes are conjugated enzymes containing one or more metal ions as prosthetic groups. Among metalloenzymes, metal ions are generally the active center of the enzyme, and most of the enzymes containing zinc, iron and copper, such as iron metalloenzyme-cytochrome C, and also enzymes containing other metal ions such as molybdenum and manganese. For example, cytochrome oxidase contains copper ions in addition to iron ions.
Disclosure of Invention
The first purpose of the invention is to provide a preparation method of carbon-based nano hybrid material.
A second object of the invention is to provide a material obtained according to the above process.
A third object of the invention is to provide the use of the above-mentioned materials.
The purpose of the invention is realized by the following technical scheme:
a preparation method of a carbon-based nano hybrid material comprises the following steps:
(1) dissolving 2.0g of carbon source citric acid and 0.6-1.0g of nitrogen source urea in water, heating and evaporating the obtained mixed solution to dryness, continuously heating and keeping the temperature for 2min, then cooling to room temperature to obtain nitrogen-doped carbon quantum dots (NCQDs), dispersing the nitrogen-doped carbon quantum dots in water, performing dialysis treatment, and refrigerating the obtained nitrogen-doped carbon quantum dot solution for later use;
(2) mixing 10mL of the nitrogen-doped carbon quantum dots (NCQDs) solution obtained in the step (1) with 2mL of a phenylalanine amide Ligand (LPN) solution, respectively adding 5mg of carboxyl activating agents 1-ethyl- (3-dimethylaminopropyl) carbonyldiimine hydrochloride (EDC), 1-Hydroxybenzotriazole (HOBT) and 4-Dimethylaminopyridine (DMAP), stirring for reaction, and then performing dialysis treatment to obtain an LPQDs solution for cold storage for later use;
(3) adding 2mL of Cu (NO) into the LPQDs solution obtained in the step (2)3)2And (3) reacting the solution to obtain the Cu (II) -LPQDs carbon-based nano hybrid material.
Further, in the step (1), the water is ultrapure water, a carbon source of citric acid and a nitrogen source of urea are dissolved in 10mL of water, and nitrogen-doped carbon quantum dots are dispersed in 100mL of water;
the temperature for heating and drying the mixed solution is 120 ℃, and the temperature is increased to 180-220 ℃;
the dialysis treatment method comprises dialyzing with 1KD dialysis bag for 12 h;
the refrigeration temperature was 4 ℃.
Further, in the step (2), the concentration of the solution of the phenylalanine amide Ligand (LPN) is 10 mmol/L;
the stirring reaction time is 12 hours;
the dialysis treatment method comprises dialyzing with 1KD dialysis bag for 12 h;
the refrigeration temperature was 4 ℃.
Further, in the step (3), Cu (NO)3)2The concentration of the solution is 10 mmol/L;
the reaction time was 4 h.
The carbon-based nano hybrid material prepared by any one of the methods.
The carbon-based nano hybrid material prepared by any method is used for detecting H in human cervical cancer cells2O2。
In the invention, the NCQDs have amino groups and a large number of carboxyl groups on the surface, the LPN ligand containing the amino groups is modified on the NCQDs in a chemical bond mode, LPN ligand molecules are successfully grafted on the NCQDs, and N atoms in the LPN amino functional groups and Cu are utilized2+Coordinating, generating toolCu (II) -LPQDs carbon-based nano hybrid material with peroxidase-like activity utilizes the excellent catalytic activity of Cu-N bond and the synergistic effect of the Cu (II) -LPQDs carbon-based nano hybrid material and nano nitrogen-doped carbon quantum dots, and passes through TMB-H2O2System implementation H2O2A dual visualization/fluorescence detection process of molecules.
The invention has the following beneficial effects:
(1) citric acid is used as a carbon source, urea is used as a nitrogen source, and a non-traditional high-temperature high-pressure hydrothermal reaction is adopted to prepare NCQDs, so that a high-toxicity chemical reagent is avoided, the fluorescence property of carbon quantum dots is improved by doping nitrogen elements, and the NCQDs with good fluorescence property are prepared and used for constructing substrates of metalloenzymes;
(2) compared with noble metal enzyme catalysts (Au, Ag, Pt and Pd) (DFT theoretical calculation), the invention adopts non-noble metal Cu as a catalytic active center, and the catalytic performance is superior to that of the noble metal enzyme catalysts;
(3) the Cu (II) -LPQDs carbon-based nano hybrid material has peroxidase-like activity and plays an important role in sensing;
(4) cu (II) -LPQDs carbon-based nano hybrid material can be used for H in human cervical cancer cells (Hela cells)2O2Detection of (3).
Drawings
FIG. 1 is a scheme for preparing NCQDs according to the present invention;
FIG. 2 is a schematic diagram of the preparation route of Cu (II) -LPQDs carbon-based nano-hybrid materials of the invention;
FIG. 3 is a transmission electron micrograph and an XPS characterization of NCQDs of the present invention;
FIG. 4 is an infrared spectrum of NCQDs of the present invention and conventional CQDs;
FIG. 5 is a UV spectrum of NCQDs of the present invention and conventional CQDs;
FIG. 6 is a graph showing fluorescence spectra of NCQDs of the present invention and conventional CQDs;
FIG. 7 shows that the Cu (II) -LPQDs carbon-based nano hybrid material of the invention is used for H in human cervical cancer cells (Hela cells)2O2Detection of (3).
Detailed Description
In order to further highlight the objects, technical solutions and advantages of the present invention, the present invention is further described with reference to the following examples, but the present invention is not limited to the scope of the examples.
Example 1
The preparation method of the Cu (II) -LPQDs carbon-based nano hybrid material comprises the following steps:
(1) 2.0g of citric acid and 0.8g of urea are weighed, mixed and dissolved in 10mL of ultrapure water to form a uniform mixed solution, wherein citric acid is used as a carbon source, and urea is used as a nitrogen source. The mixed system is placed in an electric heating jacket and heated to 120 ℃, after the solvent is evaporated to dryness, the temperature is raised to 180 ℃, the temperature is kept for 2 minutes, and the mixed system is cooled to the room temperature. The brown viscous oily substance NCQDs is obtained, ultrasonically dispersed in 100mL of ultrapure water, dialyzed for 12 hours by a 1KD dialysis bag and stored at 4 ℃ for later use.
(2) 10mL of the prepared NCQDs were mixed with 2mL of LPN (10mmol/L) in a 50mL round-bottomed flask, and 5mg each of carboxyl activating agents 1-ethyl- (3-dimethylaminopropyl) carbonyldiimine hydrochloride (EDC), 1-Hydroxybenzotriazole (HOBT) and 4-Dimethylaminopyridine (DMAP) was added. Stirring to react for 12 hours, dialyzing for 12 hours by a dialysis bag with 1KD to obtain brown yellow liquid LPQDs, and storing at 4 ℃ for later use.
(3) 2mL of Cu (NO) was added to the prepared LPQDs3)2(10mmol/L) and reacting for 4 hours to obtain the final product Cu (II) -LPQDs carbon-based nano hybrid material.
The material is used for detecting H in fresh milk and human cervical cancer cells (Hela cells)2O2。
Example 2
The preparation method of the Cu (II) -LPQDs carbon-based nano hybrid material comprises the following steps:
(1) 2.0g of citric acid and 0.6g of urea are weighed, mixed and dissolved in 10mL of ultrapure water to form a uniform mixed solution, wherein citric acid is used as a carbon source, and urea is used as a nitrogen source. The mixed system is placed in an electric heating jacket and heated to 120 ℃, after the solvent is evaporated to dryness, the temperature is raised to 180 ℃, the temperature is kept for 2 minutes, and the mixed system is cooled to the room temperature. The brown viscous oily substance NCQDs is obtained, ultrasonically dispersed in 100mL of ultrapure water, dialyzed for 12 hours by a 1KD dialysis bag and stored at 4 ℃ for later use.
(2) 10mL of the prepared NCQDs were mixed with 2mL of LPN (10mmol/L) in a 50mL round-bottomed flask, and 5mg each of carboxyl activating agents 1-ethyl- (3-dimethylaminopropyl) carbonyldiimine hydrochloride (EDC), 1-Hydroxybenzotriazole (HOBT) and 4-Dimethylaminopyridine (DMAP) was added. Stirring to react for 12 hours, dialyzing for 12 hours by a dialysis bag with 1KD to obtain brown yellow liquid LPQDs, and storing at 4 ℃ for later use.
(3) 2mL of Cu (NO) was added to the prepared LPQDs3)2(10mmol/L) and reacting for 4 hours to obtain the final product Cu (II) -LPQDs carbon-based nano hybrid material.
The material is used for detecting H in fresh milk and human cervical cancer cells (Hela cells)2O2。
Example 3
The preparation method of the Cu (II) -LPQDs carbon-based nano hybrid material comprises the following steps:
(1) 2.0g of citric acid and 0.6g of urea are weighed, mixed and dissolved in 10mL of ultrapure water to form a uniform mixed solution, wherein citric acid is used as a carbon source, and urea is used as a nitrogen source. The mixed system is placed in an electric heating jacket and heated to 120 ℃, after the solvent is evaporated to dryness, the temperature is raised to 180 ℃, the temperature is kept for 2 minutes, and the mixed system is cooled to the room temperature. The brown viscous oily substance NCQDs is obtained, ultrasonically dispersed in 100mL of ultrapure water, dialyzed for 12 hours by a 1KD dialysis bag and stored at 4 ℃ for later use.
(2) 10mL of the prepared NCQDs were mixed with 2mL of LPN (10mmol/L) in a 50mL round-bottomed flask, and 5mg each of carboxyl activating agents 1-ethyl- (3-dimethylaminopropyl) carbonyldiimine hydrochloride (EDC), 1-Hydroxybenzotriazole (HOBT) and 4-Dimethylaminopyridine (DMAP) was added. Stirring to react for 12 hours, dialyzing for 12 hours by a dialysis bag with 1KD to obtain brown yellow liquid LPQDs, and storing at 4 ℃ for later use.
(3) 2mL of Cu (NO) was added to the prepared LPQDs3)2(10mmol/L) and reacting for 4 hours to obtain the final product Cu (II) -LPQDs carbon-based nano hybrid material.
The material is used for detecting H in fresh milk and human cervical cancer cells (Hela cells)2O2。
Example 4
The preparation method of the Cu (II) -LPQDs carbon-based nano hybrid material comprises the following steps:
(1) 2.0g of citric acid and 0.6g of urea are weighed, mixed and dissolved in 10mL of ultrapure water to form a uniform mixed solution, wherein citric acid is used as a carbon source, and urea is used as a nitrogen source. The mixed system is placed in an electric heating jacket and heated to 120 ℃, after the solvent is evaporated to dryness, the temperature is raised to 180 ℃, the temperature is kept for 2 minutes, and the mixed system is cooled to the room temperature. The brown viscous oily substance NCQDs is obtained, ultrasonically dispersed in 100mL of ultrapure water, dialyzed for 12 hours by a 1KD dialysis bag and stored at 4 ℃ for later use.
(2) 10mL of the prepared NCQDs were mixed with 2mL of LPN (10mmol/L) in a 50mL round-bottomed flask, and 5mg each of carboxyl activating agents 1-ethyl- (3-dimethylaminopropyl) carbonyldiimine hydrochloride (EDC), 1-Hydroxybenzotriazole (HOBT) and 4-Dimethylaminopyridine (DMAP) was added. Stirring to react for 12 hours, dialyzing for 12 hours by a dialysis bag with 1KD to obtain brown yellow liquid LPQDs, and storing at 4 ℃ for later use.
(3) 2mL of Cu (NO) was added to the prepared LPQDs3)2(10mmol/L) and reacting for 4 hours to obtain the final product Cu (II) -LPQDs carbon-based nano hybrid material.
The material is used for detecting H in fresh milk and human cervical cancer cells (Hela cells)2O2。
Example 5
The preparation method of the Cu (II) -LPQDs carbon-based nano hybrid material comprises the following steps:
(1) 2.0g of citric acid and 0.6g of urea are weighed, mixed and dissolved in 10mL of ultrapure water to form a uniform mixed solution, wherein citric acid is used as a carbon source, and urea is used as a nitrogen source. The mixed system is placed in an electric heating jacket and heated to 120 ℃, after the solvent is evaporated to dryness, the temperature is raised to 180 ℃, the temperature is kept for 2 minutes, and the mixed system is cooled to the room temperature. The brown viscous oily substance NCQDs is obtained, ultrasonically dispersed in 100mL of ultrapure water, dialyzed for 12 hours by a 1KD dialysis bag and stored at 4 ℃ for later use.
(2) 10mL of the prepared NCQDs were mixed with 2mL of LPN (10mmol/L) in a 50mL round-bottomed flask, and 5mg each of carboxyl activating agents 1-ethyl- (3-dimethylaminopropyl) carbonyldiimine hydrochloride (EDC), 1-Hydroxybenzotriazole (HOBT) and 4-Dimethylaminopyridine (DMAP) was added. Stirring to react for 12 hours, dialyzing for 12 hours by a dialysis bag with 1KD to obtain brown yellow liquid LPQDs, and storing at 4 ℃ for later use.
(3) 2mL of Cu (NO) was added to the prepared LPQDs3)2(10mM) and reacting for 4 hours to obtain the final product Cu (II) -LPQDs carbon-based nano hybrid material.
The material is used for detecting H in fresh milk and human cervical cancer cells (Hela cells)2O2。
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. A preparation method of a carbon-based nano hybrid material is characterized by comprising the following steps:
(1) dissolving 2.0g of carbon source citric acid and 0.6-1.0g of nitrogen source urea in water, heating and evaporating the obtained mixed solution to dryness, continuously heating and keeping the temperature for 2min, then cooling to room temperature to obtain nitrogen-doped carbon quantum dots, dispersing the nitrogen-doped carbon quantum dots in water, carrying out dialysis treatment, and refrigerating the obtained nitrogen-doped carbon quantum dot solution for later use;
(2) mixing 10mL of the nitrogen-doped carbon quantum dot solution obtained in the step (1) with 2mL of a phenylalanine amide ligand solution, respectively adding 5mg of each of a carboxyl activating agent 1-ethyl- (3-dimethylaminopropyl) carbonyldiimine hydrochloride, 1-hydroxybenzotriazole and 4-dimethylaminopyridine, carrying out stirring reaction, then carrying out dialysis treatment, and refrigerating the obtained LPQDs solution for later use;
(3) adding 2mL of Cu (NO) into the LPQDs solution obtained in the step (2)3)2And (3) reacting the solution to obtain the Cu (II) -LPQDs carbon-based nano hybrid material.
2. The preparation method of the carbon-based nano-hybrid material according to claim 1, wherein in the step (1), the carbon source of citric acid and the nitrogen source of urea are dissolved in 10mL of water, the nitrogen-doped carbon quantum dots are dispersed in 100mL of water, and the water is ultrapure water;
the temperature for heating and drying the mixed solution is 120 ℃, and the temperature is increased to 180-220 ℃;
the dialysis treatment method comprises dialyzing with 1KD dialysis bag for 12 h;
the refrigeration temperature was 4 ℃.
3. The preparation method of carbon-based nano-hybrid material according to claim 1, wherein in the step (2), the concentration of the solution of the phenylalanine amide ligand is 10 mmol/L;
the stirring reaction time is 12 hours;
the dialysis treatment method comprises dialyzing with 1KD dialysis bag for 12 h;
the refrigeration temperature was 4 ℃.
4. The method for preparing carbon-based nano-hybrid material according to claim 1, wherein in the step (3), Cu (NO)3)2The concentration of the solution is 10 mmol/L;
the reaction time was 4 h.
5. Carbon-based nanohybrid materials prepared according to the process of any of claims 1 to 4.
6. Use of carbon-based nano-hybrid material prepared according to any one of claims 1 to 4 for detecting H in human cervical cancer cells2O2。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710805819.9A CN107652970B (en) | 2017-09-08 | 2017-09-08 | Preparation method and application of carbon-based nano hybrid material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710805819.9A CN107652970B (en) | 2017-09-08 | 2017-09-08 | Preparation method and application of carbon-based nano hybrid material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107652970A CN107652970A (en) | 2018-02-02 |
| CN107652970B true CN107652970B (en) | 2020-12-25 |
Family
ID=61129415
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710805819.9A Active CN107652970B (en) | 2017-09-08 | 2017-09-08 | Preparation method and application of carbon-based nano hybrid material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107652970B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110361371B (en) * | 2019-08-06 | 2021-06-22 | 福州大学 | Fluorescence sensor based on nitrogen-doped carbon dots and catalytic oxidation reaction, and construction method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102849722A (en) * | 2012-08-29 | 2013-01-02 | 中国科学院长春光学精密机械与物理研究所 | Carbon nano-dot, and preparation method and application thereof |
| CN104232084A (en) * | 2014-08-14 | 2014-12-24 | 鼎泰（湖北）生化科技设备制造有限公司 | Method for ultrasonically synthesizing hyperfluorescent carbon spots, and applications of hyperfluorescent carbon spots |
| CN106970061A (en) * | 2017-05-10 | 2017-07-21 | 青岛大学 | The preparation method of carbon point/copper nano-cluster compound ratio fluorescent dopamine probe |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107033886B (en) * | 2017-04-25 | 2019-06-25 | 安徽师范大学 | With being catalyzed and indicate difunctional fluorescent carbon point and its preparation method and application |
-
2017
- 2017-09-08 CN CN201710805819.9A patent/CN107652970B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102849722A (en) * | 2012-08-29 | 2013-01-02 | 中国科学院长春光学精密机械与物理研究所 | Carbon nano-dot, and preparation method and application thereof |
| CN104232084A (en) * | 2014-08-14 | 2014-12-24 | 鼎泰（湖北）生化科技设备制造有限公司 | Method for ultrasonically synthesizing hyperfluorescent carbon spots, and applications of hyperfluorescent carbon spots |
| CN106970061A (en) * | 2017-05-10 | 2017-07-21 | 青岛大学 | The preparation method of carbon point/copper nano-cluster compound ratio fluorescent dopamine probe |
Non-Patent Citations (2)
| Title |
|---|
| "Amino acids-incorporated nanoflowers with an intrinsic peroxidase-like activity";Zhuo-Fu Wu et al.,;《Scientific Reports》;20160301;第6卷;第22412（1-7）页 * |
| "碳点的研究进展";木合塔尔•吐尔洪等，;《分析化学》;20170131;第45卷(第1期);第139-150页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107652970A (en) | 2018-02-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kukkar et al. | Recent advances in the synthesis techniques for zeolitic imidazolate frameworks and their sensing applications | |
| Shi et al. | Covalent organic frameworks: recent progress in biomedical applications | |
| Gong et al. | Fluorescence-switchable ultrasmall fluorinated graphene oxide with high near-infrared absorption for controlled and targeted drug delivery | |
| Zou et al. | Amino Acid Coordinated Self‐Assembly | |
| Dong et al. | Metal–organic framework MIL-53 (Fe): facile microwave-assisted synthesis and use as a highly active peroxidase mimetic for glucose biosensing | |
| Ren et al. | Engineering two-dimensional metal oxides via surface functionalization for biological applications | |
| Lee et al. | Enzymatic fuel cells based on electrodeposited graphite oxide/cobalt hydroxide/chitosan composite–enzymeelectrode | |
| Shen et al. | High-performance oxygen reduction electrocatalysts derived from uniform cobalt–adenine assemblies | |
| Zhang et al. | Doped graphene: synthesis, properties and bioanalysis | |
| Samui et al. | Integration of α-amylase into covalent organic framework for highly efficient biocatalyst | |
| He et al. | Protein-supported RuO2 nanoparticles with improved catalytic activity, in vitro salt resistance, and biocompatibility: colorimetric and electrochemical biosensing of cellular H2O2 | |
| Singh et al. | Self-assembled carbon nitride/cobalt (III) porphyrin photocatalyst for mimicking natural photosynthesis | |
| Bai et al. | Metal-doped carbon nitrides: synthesis, structure and applications | |
| Zhuo et al. | Preparation of palladium/carbon dot composites as efficient peroxidase mimics for H 2 O 2 and glucose assay | |
| Munyemana et al. | Deep eutectic solvent-assisted facile synthesis of copper hydroxide nitrate nanosheets as recyclable enzyme-mimicking colorimetric sensor of biothiols | |
| Kumar et al. | Exploitation of anaerobic enriched mixed bacteria (AEMB) for the silver and gold nanoparticles synthesis | |
| Jia et al. | Electrocatalytically active cuprous oxide nanocubes anchored onto macroporous carbon composite for hydrazine detection | |
| CN109894149A (en) | A kind of composite nanostructure catalyst and its preparation and application | |
| Saikia et al. | A novel rapid synthesis of highly stable silver nanoparticle/carbon quantum dot nanocomposites derived from low-grade coal feedstock | |
| CN102847533A (en) | Microwave method for synthesizing attapulgite and palladium nanocomposite catalyst | |
| Chen et al. | AuPt bimetallic nanozymes for enhanced glucose catalytic oxidase | |
| Ranasinghe et al. | Engineered 2D materials for optical bioimaging and path toward therapy and tissue engineering | |
| Jain et al. | Highly fluorescent nitrogen-doped graphene quantum dots (N-GQDs) as an efficient nanoprobe for imaging of microbial cells | |
| CN107652970B (en) | Preparation method and application of carbon-based nano hybrid material | |
| Wu et al. | Gold nanocluster composites: Preparation strategies, optical and catalytic properties, and applications |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |