CN107647407A - A kind of method that flavonol is extracted from ginkgo leave tea - Google Patents
A kind of method that flavonol is extracted from ginkgo leave tea Download PDFInfo
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- 235000011201 Ginkgo Nutrition 0.000 title claims abstract description 70
- 235000008100 Ginkgo biloba Nutrition 0.000 title claims abstract description 70
- 150000007946 flavonol Chemical class 0.000 title claims abstract description 42
- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Natural products O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 title claims abstract description 42
- 235000011957 flavonols Nutrition 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 22
- 244000269722 Thea sinensis Species 0.000 title abstract 6
- 244000194101 Ginkgo biloba Species 0.000 title 1
- 241000218628 Ginkgo Species 0.000 claims abstract description 63
- 239000007788 liquid Substances 0.000 claims abstract description 40
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000000843 powder Substances 0.000 claims abstract description 27
- 239000011347 resin Substances 0.000 claims abstract description 26
- 229920005989 resin Polymers 0.000 claims abstract description 26
- 238000000605 extraction Methods 0.000 claims abstract description 25
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 17
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 17
- 238000000194 supercritical-fluid extraction Methods 0.000 claims abstract description 14
- 229920002292 Nylon 6 Polymers 0.000 claims abstract description 12
- 239000000287 crude extract Substances 0.000 claims abstract description 11
- 238000001035 drying Methods 0.000 claims abstract description 9
- 241000592346 Ginkgophyta Species 0.000 claims abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 70
- 241001122767 Theaceae Species 0.000 claims description 60
- 239000012530 fluid Substances 0.000 claims description 25
- 235000019441 ethanol Nutrition 0.000 claims description 20
- 239000000284 extract Substances 0.000 claims description 13
- 239000003480 eluent Substances 0.000 claims description 10
- 229910052799 carbon Inorganic materials 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 108010059892 Cellulase Proteins 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 5
- 229940106157 cellulase Drugs 0.000 claims description 5
- 238000005352 clarification Methods 0.000 claims description 5
- GNFTZDOKVXKIBK-UHFFFAOYSA-N 3-(2-methoxyethoxy)benzohydrazide Chemical compound COCCOC1=CC=CC(C(=O)NN)=C1 GNFTZDOKVXKIBK-UHFFFAOYSA-N 0.000 claims description 2
- 239000012459 cleaning agent Substances 0.000 claims 1
- 238000010521 absorption reaction Methods 0.000 abstract description 6
- 238000001179 sorption measurement Methods 0.000 abstract description 5
- 229930003944 flavone Natural products 0.000 abstract description 4
- 235000011949 flavones Nutrition 0.000 abstract description 4
- 150000002213 flavones Chemical class 0.000 abstract description 3
- 239000011148 porous material Substances 0.000 abstract description 3
- 239000001913 cellulose Substances 0.000 abstract description 2
- 229920002678 cellulose Polymers 0.000 abstract description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 210000004204 blood vessel Anatomy 0.000 description 3
- 239000004408 titanium dioxide Substances 0.000 description 3
- 206010020772 Hypertension Diseases 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- -1 flavone compound Chemical class 0.000 description 2
- 229930003935 flavonoid Natural products 0.000 description 2
- 150000002215 flavonoids Chemical class 0.000 description 2
- 235000017173 flavonoids Nutrition 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 241000790917 Dioxys <bee> Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000007330 LDL Lipoproteins Human genes 0.000 description 1
- 108010007622 LDL Lipoproteins Proteins 0.000 description 1
- 244000018633 Prunus armeniaca Species 0.000 description 1
- 235000009827 Prunus armeniaca Nutrition 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Botany (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention proposes a kind of method that flavonol is extracted from ginkgo leave tea, comprises the following steps：A, ginkgo leave tea are inserted in drying equipment；B, ginkgo tea powder is divided into A groups, B groups；C, A group ginkgoes tea powder is digested；D, enzymolysis liquid is subjected to ultrasonic wave extraction；E, crude extract is divided into solution A, second liquid；F, macroreticular resin is added into solution A；G, polycaprolactam resin is added into second liquid；H, B group ginkgoes tea powder is used into carbon dioxide supercritical extraction.Used by enzymolysis, ultrasonic wave extraction, physical absorption, chemisorbed, the comprehensive of carbon dioxide supercritical extraction, the flavones cellulose content not only extracted improves, it is and not high to big pore resin adsorption performance requirement, reduce operating condition, in a disguised form reduce cost, and the treating capacity of carbon dioxide supercritical extraction is few, therefore cost is also under control.
Description
Technical field
The present invention relates to extracting method, and in particular to a kind of method that flavonol is extracted from ginkgo leave tea.
Background technology
Ginkgo system Ginkgoaceae Ginkgo deciduous tree, contain a variety of physiologically active ingredients, wherein flavonoids in ginkgo leaf
Compound is important physiological activator, has liver protecting, prophylactic treatment angiocardiopathy, anti-oxidant, the anti-ageing effect of waiting for a long time.
Ginkgo leave tea take advanced technology to produce using the leaf of ginkgo, contain abundant protein, amino acid, mineral matter and dimension
The multiple nutritional components such as raw element.
Flavonol is one kind of flavone compound, is natural glycoside, is made up of a variety of sugar, including monosaccharide and disaccharide, three
Sugar and acylated sugar, its property is powder crystal shape.Flavonol, there is softening blood vessel, recover arteries elasticity, improve blood vessel
The effect of permeability, natural plants flavonol can strongly remove low-density lipoprotein in blood and on vascular wall, cholesterol and
Triglycerides, reach safety pressure, the purpose of lipid-loweringing, allow blood pressure to obtain stabilization for a long time, effectively prevent that hypertension and hypertension are sudden
Extremely.The effect of flavonol mainly has antioxidant, improves blood circulation, reduce cholesterol, kind reduction blood glucose, suppress inflammatory biology
Enzyme, all many-sides such as immunity, cleaning blood vessel are improved, be the health-care good product of three high crowds and the crowd of constitution difference.
The current extracting mode for extracting flavonoids from ginkgo leaf has organic solvent method, microwave method, supercritical fluid
Extraction, supercritical ultrasonics technology, adsorption resin method, but flavonol contains in the obtained product of organic solvent method, microwave method, supercritical ultrasonics technology
Amount is not high, and the effect of adsorption resin method depends on the absorption property of resin, and supercritical fluid extraction cost is too high, Wu Fa great
Scale is promoted, that is to say, that existing extractive technique has the defects of different, and research is a kind of to solve prior art defect
The method that flavonol is extracted from ginkgo leave tea just seems necessary.
The content of the invention
For the deficiency in the presence of prior art, the invention provides the method that flavonol is extracted from ginkgo leave tea, solution
The problem of extract yield in the prior art of having determined is not high, effect depends on resin, cost is not high.
To achieve the above object, present invention employs following technical scheme：It is a kind of that flavonol is extracted from ginkgo leave tea
Method, comprise the following steps：
A, ginkgo leave tea are inserted in drying equipment, dries 5- under conditions of 80-95 DEG C in carbon dioxide environment
10min；
B, dried ginkgo leave tea are inserted in disintegrating apparatus and crushed, the ginkgo tea powder of 80-100 mesh is obtained, by silver
Apricot tea powder is divided into A groups, B groups, and the mass ratio of A groups and B groups is 2:1；
C, A group ginkgo tea powders are placed in formation ginkgo tea liquid in pure water, cellulase is added in ginkgo tea liquid and is carried out
1.5-3.5h enzymolysis, obtain enzymolysis liquid；
D, ultrasonic wave extraction is carried out after enzymolysis liquid being added into ethanol, obtains crude extract；
E, crude extract is divided into solution A, second liquid two parts；
F, it will add in solution A and be filtered with the macroreticular resin of the weight such as solution A, using the ethanol of volumetric concentration 40% as removing
Miscellaneous solvent, obtains filtered fluid；
G, polycaprolactam resin addition second liquid is filtered, clarified with volumetric concentration 7-8% ethanol, after clarification with
Volumetric concentration 67-69% ethanol is eluted, and obtains eluent；
H, B group ginkgoes tea powder is used into carbon dioxide supercritical extraction, adds absolute ethyl alcohol as entrainer, fluid extraction
Pressure is 25-35MPa, and extraction temperature is 20-30 DEG C, and CO 2 fluid flow is 850-900L/h, extraction time 2-3h,
Obtain extract；
I, filtered fluid concentrated, distill, be dried to obtain flavonol；
J, eluent is concentrated in 50 DEG C of environment, obtains flavonol；
K, extract is subjected to rectification under vacuum, obtains flavonol.
Raw material chooses ginkgo leave tea and non-traditional extract ginkgo leaf, therefore drying can be differently configured from traditional drying process, temperature
Degree can be with slightly lower, and the time with slightly shorter, can so reduce the cost of drying process；The ginkgo leave tea powder footpath of 80-100 mesh is
0.15-0.18mm, the ginkgo tea powder in the range of this specified particle diameter is more easy to soak so as to be digested in water, so as to carry
High extraction；Physical absorption is carried out to the liquid containing flavonol by macroreticular resin, by polycaprolactam resin to containing flavones
The liquid of element carries out chemisorbed；The B group ginkgo tea powder usage amounts of carbon dioxide supercritical extraction are only the half of initial feed,
Therefore cost is under control.By enzymolysis, ultrasonic wave extraction, physical absorption, chemisorbed, carbon dioxide supercritical extraction it is comprehensive
Close and use, the flavones cellulose content not only extracted improves, and not high to big pore resin adsorption performance requirement, reduces operation bar
Part, cost is in a disguised form reduced, and the treating capacity of carbon dioxide supercritical extraction is few, therefore cost is also under control.
Further, in the step g, the volume of polycaprolactam resin is three times of second liquid.Advantageously in absorption.
Further, in the step h, the addition as the absolute ethyl alcohol of entrainer is CO 2 fluid flow
1-2%.
Further, in the step k, the operating environment of rectification under vacuum is：5-8 DEG C of vacuum 40-60KPa, temperature.
Compared to prior art, a kind of concrete slump retaining agent of the present invention has the advantages that：By digesting,
Ultrasonic wave extraction, physical absorption, the comprehensive use of chemisorbed, carbon dioxide supercritical extraction, the flavonol not only extracted contain
Amount improves, and not high to big pore resin adsorption performance requirement, reduces operating condition, in a disguised form reduces cost, and dioxy
It is few to change the treating capacity of carbon supercritical extract, therefore cost is also under control.
Embodiment
Embodiment one
A kind of method that flavonol is extracted from ginkgo leave tea, comprises the following steps：
A, ginkgo leave tea are inserted in drying equipment, dries 5min under conditions of 80 DEG C in carbon dioxide environment；
B, dried ginkgo leave tea are inserted in disintegrating apparatus and crushed, the ginkgo tea powder of 80 mesh is obtained, by ginkgo leave tea
Powder is divided into A groups, B groups, and the mass ratio of A groups and B groups is 2:1；
C, A group ginkgo tea powders are placed in formation ginkgo tea liquid in pure water, cellulase is added in ginkgo tea liquid and is carried out
1.5h enzymolysis, obtains enzymolysis liquid；
D, ultrasonic wave extraction is carried out after enzymolysis liquid being added into ethanol, obtains crude extract；
E, crude extract is divided into solution A, second liquid two parts；
F, it will add in solution A and be filtered with the macroreticular resin of the weight such as solution A, using the ethanol of volumetric concentration 40% as removing
Miscellaneous solvent, obtains filtered fluid；
G, polycaprolactam resin addition second liquid being filtered, the volume of polycaprolactam resin is three times of second liquid,
Clarified with the ethanol of volumetric concentration 7%, eluted after clarification with the ethanol of volumetric concentration 67%, obtain eluent；
H, B group ginkgoes tea powder is used into carbon dioxide supercritical extraction, adds absolute ethyl alcohol as entrainer, fluid extraction
Pressure is 25MPa, and extraction temperature is 20 DEG C, and CO 2 fluid flow is 850L/h, and the addition of absolute ethyl alcohol is titanium dioxide
The 1% of carbon fluid flow, extraction time 2h, obtains extract；
I, filtered fluid concentrated, distill, be dried to obtain flavonol；
J, eluent is concentrated in 50 DEG C of environment, obtains flavonol；
K, extract is subjected to rectification under vacuum at vacuum 40KPa, 5 DEG C of temperature, obtains flavonol.
Step i, j, k flavonol are collected, obtain the flavonol finally extracted from ginkgo leave tea.
Embodiment two
A kind of method that flavonol is extracted from ginkgo leave tea, comprises the following steps：
A, ginkgo leave tea are inserted in drying equipment, dries 10min under conditions of 95 DEG C in carbon dioxide environment；
B, dried ginkgo leave tea are inserted in disintegrating apparatus and crushed, the ginkgo tea powder of 100 mesh is obtained, by ginkgo leave tea
Powder is divided into A groups, B groups, and the mass ratio of A groups and B groups is 2:1；
C, A group ginkgo tea powders are placed in formation ginkgo tea liquid in pure water, cellulase is added in ginkgo tea liquid and is carried out
3.5h enzymolysis, obtains enzymolysis liquid；
D, ultrasonic wave extraction is carried out after enzymolysis liquid being added into ethanol, obtains crude extract；
E, crude extract is divided into solution A, second liquid two parts；
F, it will add in solution A and be filtered with the macroreticular resin of the weight such as solution A, using the ethanol of volumetric concentration 40% as removing
Miscellaneous solvent, obtains filtered fluid；
G, polycaprolactam resin addition second liquid being filtered, the volume of polycaprolactam resin is three times of second liquid,
Clarified with the ethanol of volumetric concentration 8%, eluted after clarification with the ethanol of volumetric concentration 69%, obtain eluent；
H, B group ginkgoes tea powder is used into carbon dioxide supercritical extraction, adds absolute ethyl alcohol as entrainer, fluid extraction
Pressure is 35MPa, and extraction temperature is 30 DEG C, and CO 2 fluid flow is 900L/h, and the addition of absolute ethyl alcohol is titanium dioxide
The 2% of carbon fluid flow, extraction time 3h, obtains extract；
I, filtered fluid concentrated, distill, be dried to obtain flavonol；
J, eluent is concentrated in 50 DEG C of environment, obtains flavonol；
K, extract is carried and carries out rectification under vacuum at vacuum 60KPa, 8 DEG C of temperature, obtain flavonol.
Step i, j, k flavonol are collected, obtain the flavonol finally extracted from ginkgo leave tea.
Embodiment three
A kind of method that flavonol is extracted from ginkgo leave tea, comprises the following steps：
A, ginkgo leave tea are inserted in drying equipment, dries 8min under conditions of 88 DEG C in carbon dioxide environment；
B, dried ginkgo leave tea are inserted in disintegrating apparatus and crushed, the ginkgo tea powder of 90 mesh is obtained, by ginkgo leave tea
Powder is divided into A groups, B groups, and the mass ratio of A groups and B groups is 2:1；
C, A group ginkgo tea powders are placed in formation ginkgo tea liquid in pure water, cellulase is added in ginkgo tea liquid and is carried out
2.5h enzymolysis, obtains enzymolysis liquid；
D, ultrasonic wave extraction is carried out after enzymolysis liquid being added into ethanol, obtains crude extract；
E, crude extract is divided into solution A, second liquid two parts；
F, it will add in solution A and be filtered with the macroreticular resin of the weight such as solution A, using the ethanol of volumetric concentration 40% as removing
Miscellaneous solvent, obtains filtered fluid；
G, polycaprolactam resin addition second liquid being filtered, the volume of polycaprolactam resin is three times of second liquid,
Clarified with the ethanol of volumetric concentration 7.5%, eluted after clarification with the ethanol of volumetric concentration 68%, obtain eluent；
H, B group ginkgoes tea powder is used into carbon dioxide supercritical extraction, adds absolute ethyl alcohol as entrainer, fluid extraction
Pressure is 30MPa, and extraction temperature is 25 DEG C, and CO 2 fluid flow is 875L/h, and the addition of absolute ethyl alcohol is titanium dioxide
The 1.5% of carbon fluid flow, extraction time 2.5h, obtains extract；
I, filtered fluid concentrated, distill, be dried to obtain flavonol；
J, eluent is concentrated in 50 DEG C of environment, obtains flavonol；
K, extract is carried and carries out rectification under vacuum at vacuum 50KPa, 6.5 DEG C of temperature, obtain flavonol.
Step i, j, k flavonol are collected, obtain the flavonol finally extracted from ginkgo leave tea.
Various ways are integrated and used by the present invention, that is, are improved recovery rate, reduced cost again.
Finally illustrate, the above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, although with reference to compared with
The present invention is described in detail good embodiment, it will be understood by those within the art that, can be to the skill of the present invention
Art scheme is modified or equivalent substitution, and without departing from the objective and scope of technical solution of the present invention, it all should cover at this
Among the right of invention.
Claims (4)
- A kind of 1. method that flavonol is extracted from ginkgo leave tea, it is characterised in that comprise the following steps：A, ginkgo leave tea are inserted in drying equipment, dries 5-10min under conditions of 80-95 DEG C in carbon dioxide environment；B, dried ginkgo leave tea are inserted in disintegrating apparatus and crushed, the ginkgo tea powder of 80-100 mesh is obtained, by ginkgo leave tea Powder is divided into A groups, B groups, and the mass ratio of A groups and B groups is 2:1；C, A group ginkgo tea powders are placed in formation ginkgo tea liquid in pure water, cellulase is added in ginkgo tea liquid and carries out 1.5- 3.5h enzymolysis, obtains enzymolysis liquid；D, ultrasonic wave extraction is carried out after enzymolysis liquid being added into ethanol, obtains crude extract；E, crude extract is divided into solution A, second liquid two parts；F, it will add in solution A and filtered with heavy macroreticular resins such as solution As, it is molten using the ethanol of volumetric concentration 40% as cleaning Agent, obtain filtered fluid；G, polycaprolactam resin addition second liquid is filtered, clarified with volumetric concentration 7-8% ethanol, with volume after clarification Concentration 67-69% ethanol is eluted, and obtains eluent；H, B group ginkgoes tea powder is used into carbon dioxide supercritical extraction, adds absolute ethyl alcohol as entrainer, fluid extraction pressure For 25-35MPa, extraction temperature is 20-30 DEG C, and CO 2 fluid flow is 850-900L/h, extraction time 2-3h, is obtained Extract；I, filtered fluid concentrated, distill, be dried to obtain flavonol；J, eluent is concentrated in 50 DEG C of environment, obtains flavonol；K, extract is subjected to rectification under vacuum, obtains flavonol.
- A kind of 2. method that flavonol is extracted from ginkgo leave tea as claimed in claim 1, it is characterised in that in the step g, The volume of polycaprolactam resin is three times of second liquid.
- A kind of 3. method that flavonol is extracted from ginkgo leave tea as claimed in claim 1, it is characterised in that in the step h, Addition as the absolute ethyl alcohol of entrainer is the 1-2% of CO 2 fluid flow.
- A kind of 4. method that flavonol is extracted from ginkgo leave tea as claimed in claim 1, it is characterised in that in the step k, The operating environment of rectification under vacuum is：5-8 DEG C of vacuum 40-60KPa, temperature.
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| US20080286391A1 (en) * | 2005-05-03 | 2008-11-20 | Bioplanta Arzneimittel Gmbh | Method for preparing ginkgo extracts having a low content of 4'-O-methyl pyridoxine and/or biflavones |
| CN103638071A (en) * | 2013-12-23 | 2014-03-19 | 湖南科技学院 | Efficient production method of gingko medicinal extract |
| CN104435017A (en) * | 2014-11-11 | 2015-03-25 | 常州大学 | Extraction method of flavonol in ginkgo leaves |
-
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- 2017-11-14 CN CN201711122368.5A patent/CN107647407A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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