CN107641632A - A kind of method with the carbon-based point of Microbe synthesis - Google Patents
A kind of method with the carbon-based point of Microbe synthesis Download PDFInfo
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- CN107641632A CN107641632A CN201710973061.XA CN201710973061A CN107641632A CN 107641632 A CN107641632 A CN 107641632A CN 201710973061 A CN201710973061 A CN 201710973061A CN 107641632 A CN107641632 A CN 107641632A
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Abstract
The invention provides a kind of method with the carbon-based point of Microbe synthesis, using plant as raw material, after it is fermented using microorganisms such as bacterium, fungies, cleaned with distilled water and remove microorganism, then sig water immersion is added, filter, filtrate in collection, a large amount of water miscible carbon-based points are can obtain finally by dialysis purification.The carbon-based planar dimension obtained is distributed mainly on 8 ~ 30 nanometers, and thickness is distributed mainly on 0.5 ~ 1.3 nanometer.Its surface carries substantial amounts of oxygen-containing functional group, such as carboxyl, hydroxyl.The material shows good photoluminescent property.The preparation method has the advantages that green, efficient, simple, cheap, workable.
Description
Technical field
The invention belongs to field of material preparation, and in particular to a kind of method with the carbon-based point of Microbe synthesis.
Background technology
Carbon-based point typically refers to the carbon nano-particle that size is less than 10 nanometers, is by sp2The carbon structure composition of hydridization, surface
With oxygen-containing functional groups such as carboxyl, hydroxyls.Carbon-based point is good because its surface makes it have with abundant hydrophilic radical
It is water-soluble.Compared to traditional semiconductor-quantum-point, carbon-based point has that building-up process is simple to operate, cost is low, small toxicity, optical
The advantages that energy is stable, good biocompatibility, therefore have potential application in chemical sensitisation, bio-sensing, bio-imaging etc.
Value.
Have been developed at present a variety of with different carbon sources（Such as carbon black, citric acid, glucose）The method for synthesizing carbon-based point,
Such as hydro-thermal method, laser ablation, pyrolysismethod, microwave method, electrochemical oxidation, Ultrasonic treatment etc..But these methods or more
Or it is few all come with some shortcomings, such as using laser ablation synthesize carbon-based point process the defects of non-selectivity etching be present and
Cause the introducing of toxic reagent, while may also need to use the unavoidable problems such as the professional instrument and equipment of comparison.Therefore,
Develop a kind of green, low energy consumption, environmentally friendly method synthesize carbon-based point, be carbon source at normal temperatures using thin such as using plant
The carbon-based point with fluorescent characteristic is made in the microbial fermentations such as bacterium, fungi, and the property for understanding carbon-based point in depth has actual
Meaning, while also the synthetic method for carbon-based point provides new thinking.
The content of the invention
It is an object of the invention to the deficiency for currently available technology, there is provided a kind of to prepare with the carbon-based of fluorescent characteristic
The method of point, this method is simple to operate, cost is cheap, green and yield is higher.The carbon-based planar dimension master obtained
8 ~ 15 nanometers are distributed in, thickness is distributed mainly on 0.5 ~ 1.3 nanometer, shows good photoluminescent property.
To achieve the above object, the present invention adopts the following technical scheme that：
Using plant as raw material, after it is fermented using microorganisms such as bacterium, fungies, cleaned with distilled water and remove microorganism, then
Sig water immersion is added, upper filtrate is collected after filtering, carbon-based point is obtained finally by dialysis purification.
The plant is one kind in the variety classes plants such as yulan tree leaf, osmanthus fragrans leaf, tealeaves.
The microorganisms such as the bacterium, fungi are aspergillus, aspergillus niger, Geotrichum, Cladosporium, bacillus subtilis, huge
One or more in Bacterium anthracoides, Sphingobacterium.
The dilute alkaline soln is that 0.001 ~ 1mol/L of volume fraction sodium hydroxide solution, ammonia spirit, potassium hydroxide are molten
One or more in liquid, soak time are 5 ~ 150 minutes.Oxygen-containing functional group in alkaline solution on carbon-based point is fully solved
From making carbon-based point take negative electrical charge, and be finally released in solution.
The bag filter used by dialysis purification is 1000 ~ 3500 molecular weight, and dialysis time is 24 ~ 72 hours.
A kind of carbon-based point for utilizing Microbe synthesis as described above, its planar dimension are distributed in 8 ~ 15 nanometers, thickness distribution
In 0.5 ~ 1.3 nanometer, good photoluminescent property is shown.
The remarkable advantage of the present invention is：
The present invention uses the plant being easy to get as raw material, by using the method for the microbial fermentations such as bacterium, fungi to prepare at normal temperatures
Water-soluble carbon basic point.The preparation method raw material is easy to get, and preparation process is simple, is set without external energy and special laboratory apparatus
Standby, yield is higher, and the carbon-based dot thickness of gained is homogeneous, and planar dimension is also relatively uniform, and the product of gained has well glimmering
Light property.
Brief description of the drawings
Fig. 1 is the Flied emission transmission electron microscope picture of the carbon-based point prepared by this method（It is left）And atomic force microscopy diagram（It is right）；
Illustration is the altitude curve of tangent line in atomic force microscope images（Bottom right）.
Fig. 2 is the infrared absorption spectroscopy of the carbon-based point prepared by this method.
Fig. 3 be with this method respectively under single and multiple strain fermentations prepared by carbon-based point Raman spectrum.
Fig. 4-1 is fluorescence emission of the carbon-based point prepared under being fermented using F1 and F2 under the conditions of different excitation wavelengths
Spectrum.
Fig. 4-2 is fluorescence emission of the carbon-based point prepared under being fermented using F3 and F4 under the conditions of different excitation wavelengths
Spectrum.
Fig. 4-3 is fluorescence emission of the carbon-based point prepared under being fermented using B1 and B2 under the conditions of different excitation wavelengths
Spectrum.
Fig. 4-4 is fluorescence emission of the carbon-based point prepared under being fermented using B3 and M under the conditions of different excitation wavelengths
Spectrum.
Embodiment
In order that content of the present invention easily facilitates understanding, with reference to embodiment to of the present invention
Technical scheme is described further, but the present invention is not limited only to this.
Embodiment 1
8 parts of 4.10 grams of stem teas are made into its moistening with 100 degree of distilled water immersions, tealeaves is placed in conical flask after being filtered with filter paper
In, after autoclaving, respectively by aspergillus in aseptic working platform（F1）, aspergillus niger（F2）, Geotrichum（F3）, Cladosporium
（F4）, bacillus subtilis（B1）, bacillus megaterium（B2）, Sphingobacterium（B3）7 kinds of fungies, bacterium and this 7 kinds are micro-
After biology presses mixed in equal amounts（M）It is introduced into tealeaves（Routinely inoculum concentration）, rubber stopper, ferments 7 days at normal temperatures beyond the Great Wall.By product
3 times removing microorganisms are cleaned with distillation water filtration, 0.5mol/L sodium hydroxide solution immersion 100min are then added, after filtering
Filtrate is collected, dialyses 36 hours and is purified finally by the bag filter of 3500 molecular weight, obtain carbon-based point.
Transmission electron microscope and AFM（See Fig. 1）Show that carbon-based planar dimension obtained by fermented by mixed bacterium is main
8 ~ 15 nanometers are distributed in, thickness is distributed mainly on 0.5 ~ 1.3 nanometer；Infrared spectrum（See Fig. 2）Illustrate obtained by fermented by mixed bacterium
Carbon-based point in have sp2Aromatic structure unit and its surface carries substantial amounts of carboxyl and hydroxyl；Raman spectrum（See Fig. 3）Illustrate real
The carbon-based point for testing gained has graphite lattice structure.Carbon-based point prepared by this method has good photoluminescent property（See Fig. 4-1 ~
Fig. 4-4）.
The raw material that the present invention uses is cheap and easy to get, and experimental implementation is simple and convenient, without special instrument and equipment, course of reaction
It is green, and finished product yield is higher, is adapted to large-scale industrial production.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, it should all belong to the covering scope of the present invention.
Claims (6)
- A kind of 1. method with the carbon-based point of Microbe synthesis, it is characterised in that：Using plant as raw material, make its fermentation using microorganism Afterwards, cleaned with distilled water and remove microorganism, then add sig water immersion, upper filtrate is collected after filtering, is carried finally by dialysis It is pure to obtain carbon-based point.
- A kind of 2. method with the carbon-based point of Microbe synthesis according to claim 1, it is characterised in that：The plant includes appointing One or more in what draft or xylophyta.
- A kind of 3. method with the carbon-based point of Microbe synthesis according to claim 1, it is characterised in that：The microorganism includes One or more in all bacteriums and fungi.
- A kind of 4. method with the carbon-based point of Microbe synthesis according to claim 1, it is characterised in that：The dilute alkaline soln is One or more in the mol/L of volume fraction 0.001 ~ 1 sodium hydroxide solution, ammonia spirit, potassium hydroxide solution, immersion Time is 5-150 minutes.
- A kind of 5. method with the carbon-based point of Microbe synthesis according to claim 1, it is characterised in that：It is described to be carried by dialysis Pure bag filter used is 1000 ~ 3500 molecular weight, and dialysis time is 24-72 hours.
- 6. the method for the carbon-based point of Microbe synthesis according to claim 1, it is characterised in that：The planar dimension of the carbon-based point 8-15 nanometers are distributed in, thickness distribution is in 0.5 ~ 1.3 nanometer, and surface carries oxygen-containing functional group.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109297948A (en) * | 2018-12-15 | 2019-02-01 | 侯槿瑄 | A kind of method that the carbon quantum dot that tealeaf residue extracts prepares TNP Test paper |
| CN109385275A (en) * | 2018-11-27 | 2019-02-26 | 合肥工业大学 | A method of fluorescent carbon quantum dot is prepared using organic matter anaerobic fermentation intermediate product as carbon-based material |
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Application publication date: 20180130 |