CN107619859A - Gene sequencing reaction device - Google Patents
Gene sequencing reaction device Download PDFInfo
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- CN107619859A CN107619859A CN201610561950.0A CN201610561950A CN107619859A CN 107619859 A CN107619859 A CN 107619859A CN 201610561950 A CN201610561950 A CN 201610561950A CN 107619859 A CN107619859 A CN 107619859A
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Abstract
The present invention relates to gene sequencing field, discloses a kind of gene sequencing reaction device, including gene sequencing reaction cell and temperature measuring equipment, and temperature measuring equipment includes a temperature probe；Gene sequencing reaction cell includes the first substrate and the second substrate, is provided with the first groove on the first substrate, after the first substrate is bonded with the second substrate, the first groove is reaction channel；Reagent inlet and reagent exit are provided with second substrate；Reaction channel is made up of reacting part and kink, and kink is connected with reacting part to be connected, and kink has angle with reacting part；A projection, and the raised junction being arranged on corresponding to the reacting part and kink are provided with the outer surface of second substrate；A thermometer hole is provided with projection, thermometer hole is used for temperature probe；The beneficial effects of the invention are as follows：Invention increases the wire being connected on temperature probe and reagent to enter the distance between pipeline, avoids the interference that wire and reagent enter between pipeline.
Description
Technical field
The present invention relates to gene sequencing field, more specifically to a kind of gene sequencing reaction device.
Background technology
Prior art gene sequencing reaction device generally includes gene sequencing reaction cell, temperature control equipment, temperature inspection
Survey device and adopt map device, wherein, gene sequencing reaction cell includes having reaction channel, reagent inlet and reagent exit,
Reagent inlet and reagent exit are connected with reaction channel, and pipe in communication is provided with reagent inlet and reagent exit
Road, the reagent of reagent or discharge after sequencing reaction is passed through into reaction channel by pipeline.The temperature control equipment is general
Include cooling piece, cooling piece is contacted with gene sequencing reaction cell, and temperature is carried out to gene sequencing reaction cell by cooling piece
The control of degree.Temperature-detecting device has generally comprised temperature sensor, and temperature sensor also contacts with gene sequencing reaction cell,
By detecting the temperature of gene sequencing reaction cell, the temperature of gene sequencing reaction is obtained.Adopt map device generally comprise camera lens and
Graphics processing unit, camera lens are connected on graphics processing unit, and described camera lens is used for caused by cdna collection sequencing reaction
Fluorescence.
In order to improve the accuracy of gene sequencing reaction temperature detection, temperature sensor needs to be arranged on apart from reaction channel
Closer proximity, therefore it is generally located on surface or the position directly below of reaction channel；Meanwhile in order to meet that gene sequencing is anti-
The demand of figure should be carried out adopting, avoid temperature sensor from being disturbed to caused by adopting figure, therefore leaned on, it is necessary to which temperature sensor is arranged on
It is bordering on the position of reagent inlet or reagent exit.The wire of temperature sensor is connected then with being connected to reagent inlet or reagent exit
On pipeline distance it is very near, inevitably occur interference during gene sequencing reaction, while also give gene survey
The installation and removal of sequence reaction small chamber bring inconvenience.
Therefore a kind of wire that can avoid temperature sensor and the pipeline appearance on reagent inlet or reagent exit are needed
The gene sequencing reaction device of interference.
The content of the invention
It is an object of the invention to provide a kind of gene sequencing reaction device, it is intended to solves temperature sensor in the prior art
Wire and reagent inlet or reagent exit on pipeline the problem of interfering.
The technical proposal of the invention is realized in this way, and a kind of gene sequencing reaction device, it is theed improvement is that：Including
Gene sequencing reaction cell and temperature measuring equipment, described temperature measuring equipment include a temperature probe；
The gene sequencing reaction cell includes the first substrate and the second substrate, one side of first substrate towards the second substrate
On be provided with the first groove, or the second substrate is towards being provided with the second groove, first substrate in the one side on the first substrate
After being bonded with the second substrate, the first described groove or the second groove are reaction channel；
The reagent inlet being connected with one end of the reaction channel is provided with second substrate, is set on second substrate
It is equipped with the reagent exit being connected with the other end of the reaction channel；
The reaction channel is made up of reacting part and kink, and the kink is connected with described reacting part to be connected, and is bent
Portion has angle with reacting part；
Be provided with the outer surface of the second described substrate it is one raised, and the projection be arranged on corresponding to the reacting part with it is curved
The junction of folding part；A thermometer hole is provided with the projection, and the thermometer hole is used to accommodate to the reaction in reaction channel
Reagent carries out the temperature probe of temperature detection.
In such a configuration, reagent is communicated with the reagent inlet and enters pipeline, is communicated with the reagent exit
Reagent discharge line.
In such a configuration, the temperature measuring equipment also includes being used for the wire for connecting the temperature probe.
In such a configuration, it is described when being provided with the first groove in the one side of the second substrate of the first substrate direction
The first substrate formed by cover glass and the fitting of drain piece, first groove is the through hole being arranged on drain piece, described the
Two substrates are slide, and the drain piece is between cover glass and slide.
In such a configuration, it is described when being provided with the second groove in the one side of the first substrate of the second substrate direction
The second substrate formed by drain piece and slide fitting, second groove is the through hole being arranged on drain piece, described the
One substrate is cover glass, and the drain piece is between cover glass and slide.
In such a configuration, the reaction channel is made up of a reacting part and a kink, the kink
One end is connected with one end of reacting part, and the other end of kink is connected with described reagent inlet, the reacting part it is another
One end is connected with described reagent exit.
In such a configuration, the reaction channel is made up of a reacting part and two kinks, two bending parts
Not Wei the first kink and the second kink, one end of first kink is connected with one end of reacting part, the first bending
The other end in portion is connected with described reagent inlet, and the other end of the reacting part is connected with the second described kink,
The other end of second kink is connected with described reagent exit.
Further, the angle that the kink and reacting part are in is A, and 30 °≤A≤120 °.
In such a configuration, the bearing of trend of the raised upper thermometer hole and the second described substrate are perpendicular；Or
The bearing of trend of the raised upper thermometer hole is parallel with the second described substrate.
Further, the raised structure being formed in one with described second substrate.
In such a configuration, the thermometer hole is square thermometric slot, and the bearing of trend of slot and described the
Two substrates are parallel；
The temperature probe is thin platinum thermocouple, in the described thermometric slot of the thin platinum thermocouple insertion.
Further, the two opposite side surfaces of the thermometric slot are provided with inclined spigot surface, and the thin platinum thermocouple is consolidated
Due in a fixed seat, the fixed seat corresponds to the guide end that spigot surface is provided with arc.
The beneficial effects of the present invention are：Reaction channel is made up of reacting part and kink, reagent inlet and kink
One end is connected, and projection is arranged on kink and the perpendicular corner of reacting part, the design of this structure, increases and is connected to
Wire on temperature probe enters the distance between pipeline with reagent, avoids the interference that wire and reagent enter between pipeline,
Meanwhile do not influence the measurement of temperature of reagent and adopt map device gene sequencing reaction is carried out to adopt figure.
Brief description of the drawings
Fig. 1, Fig. 2 are the structural representation of gene sequencing reaction device in one embodiment of the invention.
Fig. 3 is the dimensional structure diagram of gene sequencing reaction device in another embodiment of the present invention.
Fig. 4 is the structural representation of gene sequencing reaction cell in one embodiment of the invention.
Fig. 5 is the top view of gene sequencing reaction cell in one embodiment of the invention.
Fig. 6 is the diagrammatic cross-section at B-B in Fig. 5.
Fig. 7, Fig. 8 and Fig. 9 are the structural representation of gene sequencing reaction device in another embodiment of the present invention.
Figure 10 is the structural representation of reaction channel in one embodiment of the invention.
Figure 11 is the structural representation of one embodiment of the invention protrusions.
Figure 12 is the structural representation of another embodiment of the present invention protrusions.
Figure 13 is the structural representation of another embodiment of the present invention protrusions.
Figure 14 is the partial enlarged drawing at C in Figure 13.
Figure 15 is the embodiment of temperature probe and thermometer hole in another embodiment of the present invention.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, it is right below in conjunction with drawings and Examples
The present invention is further elaborated.
The present invention proposes first embodiment, and the present embodiment proposes a kind of gene sequencing reaction device, as shown in Figure 1, Figure 2 institute
Show, described gene sequencing reaction device includes gene sequencing reaction cell and temperature measuring equipment, and described temperature measuring equipment includes one
Temperature probe.Described gene sequencing reaction cell includes the first substrate 10 and the second substrate 20, the described court of the first substrate 10
The first groove 101 is provided with to the one side of the second substrate 20, after the first substrate 10 is bonded with the second substrate 20, the second substrate
20 by the closure of openings of the first groove 101, to form the reaction channel for being used for carrying out gene sequencing reaction；Second substrate 20
On be provided with the reagent inlet 201 and reagent exit 202 being connected with reaction channel, the reagent being passed through by reagent inlet 201,
Gene sequencing reaction is carried out in reaction channel, reagent exit 202 is then used to discharge reagent.In the present embodiment, the reaction
Passage is made up of a reacting part 102 and a kink 103, after the first substrate 10 and the fitting of the second substrate 20, the bending
The one end in portion 103 is connected with one end of reacting part 102, and the other end of kink 103 is connected with described reagent inlet 201
Logical, the other end of the reacting part 102 is connected with described reagent exit 202.And kink 103 has with reacting part 102
There is the angle of certain angle, as shown in Fig. 2 the kink 103 described in the present embodiment and described reacting part 102 are perpendicular.
Further, the outer surface of the second described substrate 20 is provided with a projection 30, and the projection 30 is arranged on corresponding to the reaction
The junction of portion 102 and kink 103, in Fig. 2, projection 30 be located at the outer surface of the second substrate 20, and be arranged on kink 103 and
The perpendicular corner of reacting part 102, a thermometer hole 301 is provided with described projection 30, and the thermometer hole 301 is used to hold
The temperature probe received to the reaction reagent progress temperature detection in reaction channel.
As shown in figure 3, be a kind of implementation illustration of gene sequencing reaction device of the present invention, it is described in the present embodiment
Reagent is connected with reagent inlet 201 and enters pipeline 2010, reagent discharge line is connected with described reagent exit 202
2020, reagent is passed through into reaction channel into pipeline 2010 by reagent, carries out gene sequencing reaction, reagent discharge line
2020 are used to discharge the reagent for flowing through reaction channel.Temperature probe is provided with thermometer hole 301, is connected with and leads on temperature probe
Line 40；In general, temperature measuring equipment also include temperature treating units, and temperature probe is connected to Temperature Treatment list by wire 40
Member.In the present embodiment, because described reaction channel is made up of reacting part 102 and kink 103, reagent inlet 201 and bending
The one end in portion 103 is connected, and projection 30 is arranged on kink 103 and the perpendicular corner of reacting part 102, and this structure is set
Meter, increase the wire 40 that is connected on temperature probe and enter the distance between pipeline 2010 with reagent, avoid wire 40 with
The interference that reagent enters between pipeline 2010, meanwhile, the measurement of temperature of reagent is not influenceed and to adopt map device anti-to gene sequencing
It should carry out adopting figure.
During gene sequencing reaction is carried out, it is also necessary to which the temperature of the reaction reagent in reaction channel is examined
Survey, the temperature of the reaction reagent is maintained within the scope of required temperature.Because reaction channel needs to be passed through reaction reagent, no
The situation of leakage can occur, the temperature sensor or temperature probe of detection temperature can not load in reaction channel, and its reason is
After temperature sensor or temperature probe load in reaction channel, the sealing of gene sequencing reaction cell can not be realized, is easily leaked
The situation of liquid；Because the first substrate 10 and the second substrate 20 are general all relatively thin, temperature probe is more difficult to be loaded in reaction channel；Separately
Outside, gene sequencing reaction cell is needing to be changed after gene sequencing reaction, even if temperature probe or temperature sensor energy
Enough load in reaction channel, when being changed to gene sequencing reaction cell, the assembly and disassembly of temperature probe are all more troublesome.In view of
The reason for above-mentioned, in the prior art in order to detect the temperature of the reaction reagent in reaction channel, more typical technical scheme is
On the surface that temperature sensor or temperature probe are abutted directly against to gene sequencing reaction cell, the reaction in reaction channel is tried
The temperature of agent measures；But the mode of this temperature survey, is a kind of indirect metering system, the temperature of temperature sensor measurement
Spend for the temperature of gene sequencing reaction chamber surface, the temperature it is not fully equal with the temperature of the reaction reagent in reaction channel
When, the result of temperature survey occurs as soon as larger error；Also, it is anti-that temperature sensor or temperature probe abut directly against gene sequencing
When answering in chamber surface, the cross-ventilation of gene sequencing reaction chamber surface can then impact to the measurement of temperature, make temperature
There is deviation in the measurement result of sensor.And in the present invention, by the setting of projection 30, temperature probe is arranged on projection 30
On thermometer hole 301 in, the temperature in thermometer hole 301 is then closer to the temperature of reagent in reaction channel, meanwhile, can be very
The good cross-ventilation for avoiding gene sequencing reaction chamber surface influences to caused by thermometric, improves gene sequencing reaction temperature and surveys
The accuracy of amount, the temperature for making to measure are closer to the temperature of the reaction reagent of gene sequencing reaction.
For described gene sequencing reaction cell, in the embodiment illustrated in fig. 1, the first substrate 10 is towards the second substrate
The first groove 101 is provided with 20 one side, after the first substrate 10 is bonded with the second substrate 20, the second substrate 20 is by the first groove
101 closure of openings, to form the reaction channel for being used for carrying out gene sequencing reaction.And for the first substrate 10 and the second substrate
20 concrete structure, the present invention propose another embodiment, as shown in figure 4, in the present embodiment, the first described substrate 10 by
Cover glass 104 and the fitting of drain piece 105 form, and first groove is the first through hole 1050 being arranged on drain piece 105, institute
It is slide to state the second substrate 20, and the drain piece 105 is between cover glass 104 and slide, when drain piece 105 is pressed together on
After between cover glass 104 and slide, cover glass 104 and slide then close first through hole 1050, with anti-described in formation
Answer passage.
In addition, for the position of described reaction channel, also with different implementations, for example, such as Fig. 5, Fig. 6 institute
Show, Fig. 5 is gene sequencing reaction cell front view, Fig. 6 be in Fig. 5 the diagrammatic cross-section at B-B, gene sequencing reaction it is small
Room includes the first substrate 10 and the second substrate 20, and second substrate 20 is recessed towards being provided with second in the one side of the first substrate 10
Groove 203, when the first substrate 10 is fitted on the second substrate 20, the first substrate 10 is by the closure of openings of the second groove 203, with shape
Into the reaction channel for carrying out gene sequencing reaction；This structure in the present embodiment, due to the thinner thickness of the second substrate 20,
The lower surface of two substrate 20 processes the second groove 203, and the reaction channel of formation is then closer to thermometer hole 301, in thermometer hole 301
Measurement of the temperature probe to temperature of reagent it is then more sensitive.In an alternative em bodiment, the second described substrate is by drain piece
Being formed with slide fitting, first substrate is cover glass, and drain piece is between cover glass and slide, described second
Groove is to be arranged on the second through hole on drain piece, the gene sequencing reaction cell of this structure, and the is processed on drain piece
Two through holes, then gene sequencing reaction cell is formed after slide, drain piece and cover glass are pressed, its processing technology is more
Simply.
The present invention proposes another embodiment, and the present embodiment proposes a kind of gene sequencing reaction device, such as Fig. 7, Fig. 8 institute
Show, the present embodiment and the difference of the embodiment described in Fig. 1 are, described reaction channel is curved by a reacting part 102 and two
Folding part 103 is formed, and two kinks 103 are respectively the first kink 1031 and the second kink 1032, the first substrate 10 and the
Two substrates fitting 20 after, the first kink 1031 is connected with one end of reacting part 102, the other end of the first kink 1031 and
Described reagent inlet 201 is connected；The other end of the reacting part 102 is connected with the second kink 1032, and described second
The other end of kink 1032 is connected with described reagent exit 202.In addition, set in the outer surface of the second described substrate 20
Two projections 30 are equipped with, one of projection 30 is correspondingly arranged at the junction of the first kink 1031 and reacting part 102, another
Individual raised 30 are correspondingly arranged at the junction of the second kink 1032 and reacting part 102.Described reagent inlet 201 is communicated with examination
Agent enters pipeline 2010, and described reagent exit 202 is communicated with reagent discharge line 2020, raised 30 thermometer hole 301
Temperature probe is inside provided with, and temperature probe is connected by wire, as shown in figure 9, the as connection of reagent inlet 201 examination
Agent enters pipeline 2010, reagent exit 202 connects the structure after reagent discharge line 2020 and temperature probe connecting wire 40
Schematic diagram.Reagent is passed through into reaction channel into pipeline 2010 by reagent, gene sequencing reaction is carried out in reaction channel.
In the present embodiment, temperature measuring equipment also includes temperature treating units and the report being electrically connected with temperature treating units
Unit is warned, two projections 30 are provided with the second substrate 20, thermometric spy is both provided with the thermometer hole 301 of each projection 30
Head, temperature probe are connected to temperature treating units by wire 40；The temperature treating units are used to receive two thermometrics simultaneously
The detected temperature of probe, and calculate temperature of the average for the temperature that two temperature probes are detected as reagent in reaction channel
Degree, makes the detection of temperature of reagent in reaction channel more accurate.Further, described temperature treating units receive two simultaneously
The temperature that temperature probe is detected, and the difference of the temperature of two temperature probe detections is calculated, to control whether alarm unit is sent out
Go out alarm signal.Specifically, being set with preset value t in the temperature treating units, the thermometric inside one of projection 30 is visited
The temperature that head is detected is T1, and the temperature that the temperature probe inside another projection 30 is detected is T2, temperature T1 and temperature T2
Difference is T, and the temperature treating units are used to be contrasted the difference T of temperature and preset value t, if T>T, temperature treating units
Send control command to alarm unit, alarm unit and then send alarm signal.
Further, described temperature treating units receive the temperature that two temperature probes are detected simultaneously, and to temperature
The compensation of trip temperature is entered in region where relatively low temperature probe.Specifically, described gene sequencing reaction device also includes adding
Hot slide, heating slide are made up of the first heating slide and the second heating slide, and the first heating slide and the second heating slide are equal
It is electrically connected with temperature treating units, the first heating slide is arranged on corresponding to the first projection on the bottom surface of the first substrate, and second
Heating slide is arranged on corresponding to the second projection on the bottom surface of the first substrate, the temperature that the temperature probe in the first projection is detected
For T3, the temperature that the temperature probe in the second projection is detected is T4, and the temperature treating units are used for temperature T3 and temperature
T4 is contrasted, if temperature T3>T4, temperature treating units then control the second heating slide, to the second relatively low temperature where raised
The region of degree carries out temperature-compensating；If temperature T3<T4, temperature treating units then control the first heating slide, institute raised to first
The region of lower temperature enter the compensation of trip temperature.
In addition, in the present embodiment, the first kink 1031, the structure of 102 and second kink of reacting part 1032 are set
Meter, increase the wire 40 being connected on temperature probe and enter the distance between pipeline 2010 with reagent, increase and be connected to temperature
The distance between wire 40 and reagent discharge line 2020 on degree probe, avoid wire 40 and reagent into pipeline 2010 it
Between interference, avoid the interference between wire 40 and reagent discharge line 2020, at the same do not influence temperature of reagent measurement and
Map device is adopted gene sequencing reaction is carried out to adopt figure.
In the embodiment shown in Fig. 1, the embodiment shown in Fig. 4 and the embodiment described in Fig. 7, described kink
103 is perpendicular with described reacting part 102, in the embodiment described in Fig. 7, the first described kink 1031 and the second bending
Portion 1032 is parallel, and the first kink 1031 and the second kink 1032 are each perpendicular to described reacting part 102.Will bending
Portion 103 is defined as included angle A with angle formed by reacting part 102, and in the above-described embodiment, described angle is 90 °, but needs
It is noted that the scope of the included angle A is 30 °≤A≤120 °.As shown in Figure 10, for bending with reaction formed by angle,
Propose an embodiment, in the present embodiment, wherein the first kink 1031 and angle formed by reacting part 102 are A1, A1=
120 °, the second kink 1032 and angle formed by reacting part 102 are A2, A2=110 °.
For the thermometer hole 301 on described raised 30, in the above-described embodiment, the bearing of trend of thermometer hole 301 and
Two substrates 20 are perpendicular.As shown in figure 11, the present invention also proposed an embodiment, the survey for the thermometer hole 301 of projection 30
Warm hole 301 is arranged in raised 30 side wall, and the bearing of trend of thermometer hole 301 is parallel with the second substrate 20, thermometer hole
301 this structure, the situation of sequencing reaction small chamber side is located at suitable for temperature probe, also, in the present embodiment, due to surveying
The bearing of trend in warm hole 301 is parallel with the second substrate 20, in the described thermometer hole 301 of temperature probe insertion, the second substrate
20 surface can play a part of guiding to the insertion of temperature probe, be easy in the described thermometer hole 301 of temperature probe insertion.
Further, in the opening of the thermometer hole 301, the chamfering playing the guiding role can also be set, be more convenient to thermometric spy
In the described thermometer hole 301 of head insertion.
For described projection 30, in the above-described embodiment, described raised 30 be square structure, still, described convex
The structure for playing 30 does not form influence to protection scope of the present invention, for example, described raised 30 can be designed as cylinder, or
In order to increase projection 30 and the contact area of the second substrate 20, projection 30 can be designed as circular platform type；It can also design according to demand
For other shapes.In addition, in the above-described embodiment, the thermometer hole 301 is circular blind hole, but the thermometer hole 301
Shape can be set according to demand, preferably, the shape of the thermometer hole 301 is identical with the profile of temperature probe, work as survey
After temperature probe is stretched into described thermometer hole 301, the outer surface of temperature probe forms good patch with the inwall of thermometer hole 301
Close, gap is larger so as to avoiding between the inwall of the outer surface of temperature probe and thermometer hole 301, influences the result of temperature survey.
It is as shown in figure 12, square in flat for projection 30 and another embodiment of heat emission hole, the thermometer hole 301 based on this
Thermometric slot, and the bearing of trend of thermometric slot is parallel with the second described substrate 20；Temperature probe described in the present embodiment
For thin platinum thermocouple 50, thin platinum thermocouple 50 is fixed in a fixed seat 501, the shape of fixed seat 501 and the shape of thermometric slot
Shape is identical, and the size for being dimensioned slightly smaller than thermometric slot of fixed seat 501, and fixed seat and thin platinum thermocouple 50 just may be inserted into
In described thermometric slot.Further, in order to lift the heat transfer efficiency between the inwall of thermometer hole 301 and temperature probe,
Heat conductive silica gel is filled with the thermometer hole 301, by the effect of heat conductive silica gel, by the heat transfer in projection 30 to the survey
On temperature probe.In alternate embodiment, as shown in Figure 13, Figure 14, Figure 14 is the partial enlarged drawing at C in Figure 13, the thermometric
The two opposite side surfaces in hole 301 are provided with inclined guide surface 3010, and the corresponding spigot surface of fixed seat 501 is provided with arcuate guide end 5010,
To facilitate fixed seat 501 more easily to insert in thermometer hole 301.As described in Figure 15, for described fixed seat 501, scheming
On the basis of embodiment described in 14, it is also proposed that an embodiment, described fixed seat 501 also have a corner cut guide end
5011, by the collective effect of corner cut guide end 5011 and house type guide end 5010, it is easy to fixed seat 501 easily to insert thermometric
In hole 301.
In above-mentioned any embodiment, described raised 30 and second structure that is formed in one of substrate 20, also, it is described
The material of second substrate 20 is PDMS, i.e. dimethyl silicone polymer.Because PDMS quality is softer, when temperature probe stretch into it is described
Thermometer hole 301 in after, temperature probe can be closely bonded with the inwall of thermometer hole 301, and the temperature on PDMS interlayers then can
Enough it is transferred on temperature probe, is realized to reaction reagent temperature in gene sequencing reaction well by the effect of thermometer hole 301
Measurement.For the laminating type of the substrate 20 of the first substrate 10 and second, the present invention proposes an example, first base
Piece 10 is close on the second substrate 20 by the way of pressing.In addition, in the embodiment described in Fig. 4, first substrate 10
Be made up of cover glass 104 and drain piece 105, second substrate 20 is slide, described cover glass 104, drain piece 105 with
And slide carries out plasma treatment on the surface for needing to be bonded, hydroxyl is formed, cover glass 104 is bonded by way of bonding
On drain piece 105, the slide is fixed on by way of bonding on drain piece 105, is realized by way of bonding each
Fitting between layer, its seal are more preferable.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.
Claims (12)
- A kind of 1. gene sequencing reaction device, it is characterised in that：Including gene sequencing reaction cell and temperature measuring equipment, described survey Warm device includes a temperature probe；The gene sequencing reaction cell includes the first substrate and the second substrate, one side of first substrate towards the second substrate On be provided with the first groove, or the second substrate is towards being provided with the second groove, first substrate in the one side on the first substrate After being bonded with the second substrate, the first described groove or the second groove are reaction channel；The reagent inlet being connected with one end of the reaction channel is provided with second substrate, is set on second substrate It is equipped with the reagent exit being connected with the other end of the reaction channel；The reaction channel is made up of reacting part and kink, and the kink is connected with described reacting part to be connected, and is bent Portion has angle with reacting part；Be provided with the outer surface of the second described substrate it is one raised, and the projection be arranged on corresponding to the reacting part with it is curved The junction of folding part；A thermometer hole is provided with the projection, and the thermometer hole is used to accommodate to the reaction in reaction channel Reagent carries out the temperature probe of temperature detection.
- 2. gene sequencing reaction device according to claim 1, it is characterised in that：Reagent is communicated with the reagent inlet Into pipeline, reagent discharge line is communicated with the reagent exit.
- 3. gene sequencing reaction device according to claim 1, it is characterised in that：The temperature measuring equipment also includes being used to connect Connect the wire of the temperature probe.
- 4. gene sequencing reaction device according to claim 1, it is characterised in that：The second substrate of the first substrate direction One side on when being provided with the first groove, the first described substrate is formed by cover glass and the fitting of drain piece, first groove For the through hole being arranged on drain piece, second substrate is slide, and the drain piece is between cover glass and slide.
- 5. gene sequencing reaction device according to claim 1, it is characterised in that：The first substrate of the second substrate direction One side on when being provided with the second groove, the second described substrate is formed by drain piece and slide fitting, second groove For the through hole being arranged on drain piece, first substrate is cover glass, and the drain piece is between cover glass and slide.
- 6. gene sequencing reaction device according to claim 1, it is characterised in that：The reaction channel is by a reacting part Formed with kink, one end of the kink is connected with one end of reacting part, the other end of kink with it is described Reagent inlet is connected, and the other end of the reacting part is connected with described reagent exit.
- 7. gene sequencing reaction device according to claim 1, it is characterised in that：The reaction channel is by a reacting part Formed with two kinks, two kinks are respectively the first kink and the second kink, one end of first kink Be connected with one end of reacting part, the other end of the first kink is connected with described reagent inlet, the reacting part it is another One end is connected with the second described kink, and the other end of second kink is connected with described reagent exit.
- 8. according to the gene sequencing reaction device described in any claim of claim 1,6,7, it is characterised in that：The bending The angle that portion and reacting part are in is A, and 30 °≤A≤120 °.
- 9. gene sequencing reaction device according to claim 1, it is characterised in that：The extension side of the raised upper thermometer hole To perpendicular with the second described substrate；OrThe bearing of trend of the raised upper thermometer hole is parallel with the second described substrate.
- 10. gene sequencing reaction device according to claim 9, it is characterised in that：The second described raised and described base The structure that piece is formed in one.
- 11. gene sequencing reaction device according to claim 1, it is characterised in that：The thermometer hole is square thermometric Slot, and the bearing of trend of slot is parallel with the second described substrate；The temperature probe is thin platinum thermocouple, in the described thermometric slot of the thin platinum thermocouple insertion.
- 12. gene sequencing reaction device according to claim 11, it is characterised in that：Two relatively of the thermometric slot Side is provided with inclined spigot surface, and the thin platinum thermocouple is fixed in a fixed seat, and the fixed seat is corresponded to and set on spigot surface There is the guide end of arc.
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| CN201610561950.0A CN107619859A (en) | 2016-07-13 | 2016-07-13 | Gene sequencing reaction device |
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| CN201610561950.0A CN107619859A (en) | 2016-07-13 | 2016-07-13 | Gene sequencing reaction device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109401940A (en) * | 2018-10-22 | 2019-03-01 | 深圳华因康基因科技有限公司 | Gene sequencing reaction cell |
| CN111575146A (en) * | 2020-04-21 | 2020-08-25 | 通用生物系统（安徽）有限公司 | Gene sequencing reaction device |
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