CN107619645B - Method for preparing dopamine-enhanced starch-based adhesive based on holoenzyme method - Google Patents
Method for preparing dopamine-enhanced starch-based adhesive based on holoenzyme method Download PDFInfo
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- CN107619645B CN107619645B CN201711057701.9A CN201711057701A CN107619645B CN 107619645 B CN107619645 B CN 107619645B CN 201711057701 A CN201711057701 A CN 201711057701A CN 107619645 B CN107619645 B CN 107619645B
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- laccase
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Abstract
The invention discloses a method for preparing a dopamine-enhanced starch-based adhesive based on a holoenzyme method, which is characterized in that starch generates aldehyde groups by using a TEMPO/laccase system, the aldehyde groups and dopamine form firm covalent bonding through aldehyde-amine condensation, and meanwhile, poly-baryl is enzymatically oxidized under the action of laccase to produce a high-bonding-strength starch-based adhesive, so that the service performance and the application range of the starch adhesive are improved.
Description
Technical Field
The invention relates to a method for preparing a dopamine-enhanced starch-based adhesive based on a holoenzyme method, in particular to a high-adhesion starch adhesive and application thereof, belonging to the technical field of macromolecules.
Background
The 20 th century and the 50 th century began to replace natural adhesives with synthetic adhesives, but the petroleum resources are gradually exhausted and the environmental protection is more and more popular, and the development and utilization of natural renewable resources for preparing adhesives are more and more important. More and more physical and chemical denaturation methods of natural materials are used to improve the overall performance of natural adhesives, especially how to improve the bond strength of starch-based adhesives.
Mussels secrete mucus which can be adsorbed on the surface of almost any substrate, and show super-strong adhesion capability. Recent studies have shown that mussels have superior adhesive capacity, which is attributed to their catecholic structure, which is contained in large amounts in the mucus. According to the characteristics, the starch macromolecule chain is modified by the catechol group to obtain the high-strength binding material with excellent binding capacity.
TEMPO and its derivatives have been widely used as mediators of laccase catalytic systems, TEMPO/laccases being able to oxidize the hydroxyl groups on glucose and its derivatives to aldehyde and carboxyl groups. Therefore, starch can be treated by an enzymatic oxidation method to generate aldehyde groups or carboxyl groups, then the starch and dopamine undergo an aldehyde-amine condensation reaction to covalently link the dopamine on a starch molecular chain, and then the laccase is used for carrying out enzymatic oxidation on poly-baryl (DOPA) on the starch molecular chain to produce the ecological and environment-friendly high-strength bonding material, so that the method can be widely applied to the fields of textile, papermaking, home decoration and the like.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a method for preparing dopamine-enhanced starch-based adhesive by using holoenzyme, wherein starch generates aldehyde group by using a TEMPO/laccase system, the aldehyde group and dopamine form firm covalent bonding through aldehyde-amine condensation, meanwhile, polybaryl is subjected to enzymatic oxidation under the action of laccase, so that the high-bonding-strength starch-based adhesive is produced, the using performance and the application range of the starch adhesive are improved, and the specific process and the steps are as follows:
30g of starch was weighed, the slurry was concentrated to 15% with 0.1M NaAc-HAc buffer pH 5.0, and laccase (2mL, 1000 UmL) was added-1) And TEMPO (2 percent, on a dry basis of starch), introducing air, reacting at 30 ℃, adding 10g of dopamine after 2 hours, stirring for 1 hour, and heating to 95 ℃ for gelatinization for 10min to obtain the dopamine-reinforced starch-based adhesive.
According to the invention, a TEMPO/laccase system is used for selectively oxidizing starch primary hydroxyl to generate aldehyde groups, a DOPA group is introduced through aldehyde-amine condensation, and meanwhile, the DOPA group is catalyzed and oxidized by laccase to prepare the dopamine-enhanced starch-based adhesive.
Detailed Description
The starch-based adhesive is prepared by adopting a holoenzyme method, and the specific embodiment is as follows:
example 1
30g corn starch was weighed, the slurry was concentrated to 15% with 0.1M NaAc-HAc buffer pH 5.0, and laccase (2mL, 1000 UmL) was added-1) And TEMPO (2 percent, on a dry basis of starch), introducing air, reacting at 30 ℃, adding 10g of dopamine after 2 hours, stirring for 1 hour, and heating to 95 ℃ for gelatinization for 10min to obtain the dopamine-reinforced starch-based adhesive.
Example 2
30g of potato starch was weighed, the slurry was concentrated to 25% with 0.1M phosphate buffer pH 6.0, and laccase (10mL, 1000 UmL) was added-1) And TEMPO (8 percent, on a dry basis of starch), introducing air, reacting at 40 ℃, adding 15g of dopamine after 8 hours, stirring for 2 hours, heating to 95 ℃, and gelatinizing for 10min to obtain the dopamine-reinforced starch-based adhesive.
Example 3
Weighing 30g cassava starch, adjusting the pulp concentration to 10% with 0.1M citric acid buffer solution with pH 4.8, adding laccase (1mL, 1000 UmL)-1) And TEMPO (1% of starch dry base), introducing air, reacting at 50 ℃, adding 5g of dopamine after 20 hours, stirring for 1 hour, heating to 95 ℃, and gelatinizing for 10min to obtain the dopamine-reinforced starch-based adhesive.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (2)
1. A method for preparing a dopamine-enhanced starch-based adhesive based on a holoenzyme method comprises the following specific processes and steps:
weighing 30g starch, concentrating the slurry to 15% with 0.1M NaAc-HAc buffer solution with pH 5.0, adding laccase 2mL, 1000UmL-1And TEMPO 2%, introducing air to the dry starch base, reacting at 30 ℃, adding 10g of dopamine after 2 hours, stirring for 1 hour, heating to 95 ℃, and gelatinizing for 10min to obtain the dopamine reinforced starch base adhesive.
2. The method of claim 1, wherein: the dopamine-reinforced starch-based adhesive is prepared from raw materials of corn starch, wheat starch, cassava starch, potato starch and corresponding modified starch.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711057701.9A CN107619645B (en) | 2017-11-01 | 2017-11-01 | Method for preparing dopamine-enhanced starch-based adhesive based on holoenzyme method |
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| CN201711057701.9A CN107619645B (en) | 2017-11-01 | 2017-11-01 | Method for preparing dopamine-enhanced starch-based adhesive based on holoenzyme method |
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| CN107619645A CN107619645A (en) | 2018-01-23 |
| CN107619645B true CN107619645B (en) | 2021-09-03 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109403089A (en) * | 2018-10-15 | 2019-03-01 | 安阳师范学院 | A kind of coating dye printing Environment-friendlyadhesive adhesive and its preparation method and application |
| CN111560226B (en) * | 2019-12-20 | 2021-06-15 | 湖南工业大学 | Starch adhesive and preparation method thereof |
| CN111040667B (en) * | 2019-12-20 | 2021-04-20 | 湖南工业大学 | Dopamine-functionalized starch adhesive and preparation method thereof |
| CN112409974B (en) * | 2020-11-19 | 2021-12-21 | 江南大学 | Amphiphilic bio-based degradable underwater adhesive and preparation method and application thereof |
| CN116607351A (en) * | 2023-05-22 | 2023-08-18 | 海南椰国食品有限公司 | Nanometer bacterial cellulose hydrophobic oil-proof paper and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103554283A (en) * | 2013-10-10 | 2014-02-05 | 西北大学 | Functional starch containing dopamine and polyethylene glycol, and preparation method and application thereof |
| CN106336829A (en) * | 2016-09-07 | 2017-01-18 | 江南大学 | Preparation method for DA (Dopamine)-based adhesive |
| CN107158453A (en) * | 2017-06-02 | 2017-09-15 | 武汉纺织大学 | A kind of preparation method of hyaluronic acid tissue adhesive |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| ES2700662T3 (en) * | 2010-11-15 | 2019-02-18 | Agrana Staerke Gmbh | Composition of starch based adhesive |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103554283A (en) * | 2013-10-10 | 2014-02-05 | 西北大学 | Functional starch containing dopamine and polyethylene glycol, and preparation method and application thereof |
| CN106336829A (en) * | 2016-09-07 | 2017-01-18 | 江南大学 | Preparation method for DA (Dopamine)-based adhesive |
| CN107158453A (en) * | 2017-06-02 | 2017-09-15 | 武汉纺织大学 | A kind of preparation method of hyaluronic acid tissue adhesive |
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