CN107610724A - Quantitative detecting method based on the full optical disk image forming system of blu-ray drives - Google Patents
Quantitative detecting method based on the full optical disk image forming system of blu-ray drives Download PDFInfo
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- CN107610724A CN107610724A CN201710656932.5A CN201710656932A CN107610724A CN 107610724 A CN107610724 A CN 107610724A CN 201710656932 A CN201710656932 A CN 201710656932A CN 107610724 A CN107610724 A CN 107610724A
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Abstract
A kind of quantitative detecting method based on the full optical disk image forming system of blu-ray drives, the quantitative detecting method are to prepare biological detection arrays on standard Blu-ray Disc；CD is read using standard blu-ray drives, by the dimension transformation of full optical disk image forming system of the light intensity signal collected Jing Guo standard blu-ray drives, feature alignment, average scaling, coordinate system transformation, forms full disc image file；The image file of generation is analyzed, just obtains the quantitative testing result of target biological molecules.This method have the advantages that more flux, high sensitivity, can field quick detection, suitable for key areas such as food security, water quality detection and medical diagnosis.
Description
Technical field
The present invention relates to a kind of analysis of imaging of biomolecules and quantitative detecting method, specifically it is a kind of based on LabVIEW,
The quantitative detecting method of the full optical disk image forming system of the standard blu-ray drives of MATLAB and C++ hybrid programmings.
Background technology
At year ends 1970, optical disc comes out, and because optical disc surface is very smooth and has good optical property, passes through
The optical disc surface of processing can be with specific binding protein, DNA probe molecule, and then can prepare detection array in optical disc surface
Structure, when CD-ROM system laser beam flying CD to CD detection array top, laser beam path is interfered, with photoelectricity two
Pole pipe measuring beam intensity will be reduced accordingly.Wherein, the optical signalling amplitude change that photodetector receives in optical head
It can be used for quantitative analysis.
Early in 2000, the Kido in the U.S. et al.（Kido H, Maquieira A, Hammock B D. Disc-
based immunoassay microarrays[J]. Analytica Chimica Acta, 2000, 411(1-2): 1-
11）The protein microarray of the fluorescence labeling based on immune response is prepared in common CD optical disc surfaces, uses Fluorescence Scanner
It is tested and analyzed, and proposes and any hardware modification can realization " biochemistry CD " signal is not carried out to CD-ROM drive
Identification and specificity read " software solution " imagination.2003, La Clair et al.（La Clair J J,
Burkart M D. Molecular screening on a compact disc[J]. Organic & Biomolecular
Chemistry, 2003, 1(18): 3244-9）Based on standard CD-ROM drive, the software comparison write by using design is divided
Analyse the initial data wrong data corresponding with the CD after molecule association reaction of CD, for quantitative screening go out ligand-
Association reaction between protein.This method screens the part and biology point of CD optical disc surfaces expression by error mensuration program
Son, it is accurate without DVD and BD CD-ROM drives due to using CD drive, and because PC is incompatible with there is pole solvent, to CD-R surfaces
Activated by phosphorylation attaching ligand molecule to be extremely difficult in acetonitrile.
The Potyrailo in the U.S. et al.（Radislav A. Potyrailo, William G. Morris, Andrew
M. Leach, et al. Analog Signal Acquisition from Computer Optical Disk Drives
for Quantitative Chemical Sensing[J]. Analytical Chemistry, 2006, 78(16):
5893-9）From CD-ROM driver's printed circuit board extract optical head photodetector analog signal, and Usage data collection card collection and
LabVIEW writes program and CD-ROM drive is controlled and is imaged, and realizes the quantitative detection of the metal ion of CD optical disc surfaces.But this
Kind system only realizes array image-forming, fails to realize full optical disk image forming, and flux is not also high, does not utilize individual light sufficiently
Disk, the redundancy of experiment number can be caused.In patent（Radislav Alexandrovich Potyrailo. Sensor
systems and methods for quantifications of physical parameters, chemical and
biochemical volatile and nonvoiatile compounds in fluids:The U.S., the B2 of US 7,170,609
[P].2007-1-30）In, Potyrailo et al. extracts light intensity signal from photoelectric detector, and transmission is quantified by analog-to-digital conversion
The intensity of light, so as to obtain the concentration of compound on CD.But, not to full optical disk image forming in the patent.
Hispanic Maquieira research groups（Morais S, Carrascosa J, Mira D, et al.
Microimmunoanalysis on standard compact discs to determine low abundant
compounds[J]. Analytical Chemistry, 2007, 79(20): 7628-7635.）It is then using semi-transparent semi-reflecting
Special CD, an Optical Sensing Trigger and plane photodiode receiver are added above CD-ROM drive, extracts light in real time
The transmitted light intensity signal of conversion zone is analyzed on disk.This method is except that will set special CD, it is also necessary in CD-ROM drive
Other hardware units are installed, it is more bothersome, and the business softwares such as dependence photoshop are needed in post-processing data.
Various forms of transformations of CD-ROM drive, new molecular recognition and the platform technology of detection are become, still, due to
The particularity of transformation, these transformations can influence CD-ROM drive and read the ability of CD or even destroy CD-ROM drive, and it is special that some also needs to make
CD, these all largely reduce the advantage of molecular recognition and detection technique based on CD/CD-ROM drive.The present invention
The method that people proposes neither transforms CD, realizes full optical disk image forming again, while solves to detect in standard CD-ROM drive detection method and lead to
The problem of amount is limited.
The content of the invention
It is an object of the invention to based on blu-ray drives read-write theory and CD storage technique, by extracting, gathering
The light intensity signal of photoelectric detector inside standard blu-ray drives optical head, using researching and developing under a windows environment based on standard
The full optical disk image forming system of blu-ray drives, biomolecule high accuracy, high-throughout quick determination method are realized, and a kind of base is provided
In the quantitative detecting method of the full optical disk image forming system of blu-ray drives.
To achieve these goals, the present invention provides a kind of quantitative detection side based on the full optical disk image forming system of blu-ray drives
Method, methods described follow these steps to carry out：
In Blu-ray Disc polycarbonate surface biological detection arrays are formed using microflow control technique；
CD is scanned using blu-ray drives, during CD scanning, is adopted using data collecting card from optical head photoelectric detector
Full optical disk image forming system of the data synchronization transmissions collected to standard blu-ray drives on computer；
The data collected are subjected to dimension transformation using the full optical disk image forming system of standard blu-ray drives, feature is alignd, average contracts
Put, full disc image file is formed after coordinate system transformation；
Image analysis module using the full optical disk image forming system of standard blu-ray drives is entered to the pixel value of image file biologic array
Row linear fit, obtain target biological molecules concentration；
Realize imaging analysis and quantitative detection.
In the above-mentioned technical solutions, the further additional technical feature of the present invention is：
The quantitative detecting method is to put the CD after biological respinse well, and the light of biological respinse is not being done with metallochrome marker pen
Disk data field, using CD radius as directrix, characteristic value of the mark triggers band as gathered data.
The quantitative detecting method be will by gather storage the files of I 16 in one-dimensional data according to sampling length gradually
Reduction sampling process simultaneously sequentially inputs elliptic filter, and using LPF, filtered data are stored by interception position, storage
Data are 2-D data in file afterwards, realize gathered data dimension transformation, and it is right that the 2-D data after dimension transformation is passed through
Single acquisition data carry out Envelope Analysis, meet characteristic quantity position mark to width and amplitude, and intercept the data of corresponding length
Once store so that 2-D data completes feature alignment, the data procession format length after alignment is gone by setting
Number and columns expand or compressed in a manner of rounding nearby so that data realize that average scales.
The quantitative detecting method is the image generation module in the full optical disk image forming system of standard blu-ray drives by average
2-D data after scaling, by the operation of below equation：
Wherein：M, n are original coordinates；X, y are to meet actual pixels coordinate value；R is imaging region start radius；Δ r is adjacent
The space length of two rows；Δ θ is the angle that adjacent two point data corresponds to space in data line, realizes each data convert
Relevant position onto full disc image.
The quantitative detecting method is the Analysis of test results module in the full optical disk image forming system of standard blu-ray drives, will
Tif format image files are converted into bianry image, then using the data interaction function of Analysis of test results module, when click pair
When answering the arbitrary region in bianry image, pixel average size in the region enveloping space can be provided in display box, is being detected
It is separately added into Standard-concentration and Standard-pixel value in interpretation of result module and inserts mark
The concentration and respective image pixel value of quasi- sample, testing sample pixel value is inserted in Sample-pixel value, is set up
Can be to provide testing result into rear click ' the Fitting Calculation ' button.
This method with standard CD-ROM drive and carries standard blu-ray drives based on blu-ray drives read principle and memory technology
The computer of full optical disk image forming system be platform, overcoming transformation CD-ROM drive, time-consuming, it is expensive the problems such as, with existing biology
Molecular method quantification detection method is compared, it is possible to achieve full optical disk image forming, detection flux are higher.
What this method utilized is standard blu-ray drives, and compared with CD, DVD drive, the focal beam spot diameter of its laser is smaller,
Detection resolution is higher, has higher detection sensitivity.
The micro-concentrations of this method heavy metal etc. in harmful components, residues of pesticides, water imaging analysis food,
The fields such as clinical examination, diagnosis, food industry and individual medical treatment are can be widely applied to, before there is boundless market
Scape.
Brief description of the drawings
Fig. 1 is the full optical disk image forming system schematic of this method standard blu-ray drives.
Fig. 2 is the full optical disk image forming figure of this method biology dot matrix.
Fig. 3 is the matched curve figure of this method standard sample and testing sample.
Embodiment
The embodiment of the present invention is made further instructions below.
Implement a kind of quantitative detecting method based on the full optical disk image forming system of blu-ray drives provided by the present invention, including this
What inventive method used puts CD-ROM drive gathered data into there will be biologic array with the CD for carrying out triggering band, the data after collection
By dimension transformation, feature alignment, average scaling, coordinate system transformation, full disc image file is formed, to the image file of generation
The concentration of target biological molecules is obtained after data interaction, the Fitting Calculation, specific detection method follows these steps to carry out：
（1）The biological respinse of CD：In Blu-ray Disc polycarbonate surface biological detection arrays are formed using microflow control technique.
（2）Mark triggers band：CD after biological respinse is put well, biological respinse is not being done with metallochrome marker pen
Data of optical disk area, using the radius of CD as directrix, characteristic value of the mark triggers band as gathered data.
（3）Gathered data：The CD that biologic array is formed after biological respinse is put into external blu-ray drives, utilized
The mistake that tracks in PlexUTILITIES softwares（TE）And focal error（FE）, make CD start to rotate with 6 times of rotating speeds, data
In gatherer process, full optical disk image forming system parameter configuration in data acquisition of standard blu-ray drives：Coupled modes use
AC coupled, trigger source are ATR triggerings, and triggering direction is that lower edges trigger, and passage is arranged to ± 1000mv, sampling frequency
Rate is 2MHz, and clock source is internal clocking.Data collecting card starts to gather the light intensity signal of photoelectric detector, while will collect
Full optical disk image forming system of the data transfer to standard blu-ray drives, the data storage collected is I16 files.
（4）Gathered data processing：One-dimensional data in I16 storage files after collection is gradually reduced according to sampling length
Sampling process simultaneously sequentially inputs elliptic filter, uses LPF, the mv of measurement range selection ± 1000, filtered number during filtering
Stored according to by interception position, data are two dimension in data end per treatment insertion newline storage, the file after storage
Data, realize the dimension transformation of gathered data.To the 2-D data after dimension transformation by being carried out to the data of single acquisition
Envelope Analysis, meets width and amplitude the position mark of characteristic quantity, and the data for intercepting corresponding length once store so that two
Dimension data completes feature alignment, and the data storage after alignment is txt forms.When 2-D data feature is alignd, setting triggering band
It is upper and lower be limited to 10000 ~ 25000, individual pen data volume up and down be limited to 100 ~ 250 and trigger band threshold value.After alignment
Data procession format length, i.e., expanded or compressed in a manner of rounding nearby by setting line number and columns so that data
Realize that average scales.
（5）Full optical disk image forming and quantitative detection：In the image generation module of the full optical disk image forming system of standard blu-ray drives
2-D data after average is scaled, by the conversion from rectangular coordinate system to polar coordinate system, realize that each data convert arrives
Relevant position on disc image, i.e., by a rectangular image by forming a doughnut after rectangular coordinates transformation to polar coordinates
Picture, the closer actual CD of image of generation.When image generates, it is necessary to set the CD center of circle to the most short distance of triggering band
From the ranks number of the 2-D data after R0, triggering band length Δ R, alignment columns and average scaling, so as to obtain original adopt
The two pseudo- chromaticity diagrams generated after collection data processing optimization（Jpg and tif）.In the full optical disk image forming system of standard blu-ray drives
Tif format image files are converted into bianry image by Analysis of test results module, using data interaction function when click corresponding two
When being worth the arbitrary region in image, pixel average in the region enveloping space can be provided in Sample_intensity display boxes
Size.The concentration for inserting standard sample is separately added into Standard-intensity and Standard-concentration
With respective image gray value, testing sample gray value is inserted in Sample-intensity, ' fitting meter is clicked on after being provided with
Calculate ' button can be to provide testing result.
Below with determinand aflatoxins（AFB1）Exemplified by, a kind of imaging of biomolecules analysis of the present invention is further described
And quantitative detecting method, its embodiment follow these steps to carry out：
Step 1: the biological respinse of CD
In Blu-ray Disc polycarbonate surface biological detection arrays are formed using microflow control technique.
Step 2: mark triggers band
CD after biological respinse is put well, with metallochrome marker pen in the data of optical disk area for not doing biological respinse, with CD
Radius is directrix, characteristic value of the mark triggers band as gathered data.
Step 3: gathered data
The CD that biologic array is formed after biological respinse is put into external blu-ray drives, using in PlexUTILITIES softwares
Track mistake（TE）And focal error（FE）, make CD start to rotate with 6 times of rotating speeds, in data acquisition, standard blue light light
The full optical disk image forming system parameter configuration in data acquisition driven：Coupled modes use AC coupled, and trigger source is ATR
Triggering, triggering direction is that lower edges trigger, and passage is arranged to ± 1000mv, sample frequency 2MHz, and clock source is inside
Clock.Data collecting card starts to gather the light intensity signal of photoelectric detector, while gives the data transfer collected to standard blue light
The full optical disk image forming system of CD-ROM drive, the data storage collected are I16 files.
Step 4: gathered data is handled
One-dimensional data in I16 storage files after collection is gradually reduced into sampling process according to sampling length and sequentially input ellipse
Circle wave filter, LPF use during filtering, the mv of measurement range selection ± 1000, filtered data are by interception position storage, every
Secondary processing data end insertion newline stores, and data are 2-D data in the file after storage, realize gathered data
Dimension transformation.To the 2-D data after dimension transformation by carrying out Envelope Analysis to the data of single acquisition, to width and amplitude
Meet the position mark of characteristic quantity, and the data for intercepting corresponding length once store so that 2-D data completes feature alignment, right
Data storage after neat is txt forms.When 2-D data feature is alignd, setting triggering band it is upper and lower be limited to 10000 ~ 25000,
Individual pen data volume up and down be limited to 100 ~ 250 and trigger band threshold value be -0.2.By the data procession length after alignment
Format, i.e., be 1471 by setting line number and columns, expanded in a manner of nearly rounding or compressed so that data realize average
Scaling.
Step 5: full optical disk image forming and quantitative detection
2-D data after the image generation module of the full optical disk image forming system of standard blu-ray drives scales average, by by
Conversion of the rectangular coordinate system to polar coordinate system, relevant position of each data convert to disc image is realized, i.e., by one
Rectangular image is by forming a doughnut picture, the closer actual CD of image of generation after rectangular coordinates transformation to polar coordinates.
When image generates, it is necessary to the beeline R0 that the CD center of circle to triggering band is set be 2.4, triggering band length Δ R be
3.6th, the columns that aligns be 98 and average scaling after the ranks number of 2-D data be 1471, so as to obtain at acquired original data
The two pseudo- chromaticity diagrams generated after reason optimization（Jpg and tif）.In the testing result of the full optical disk image forming system of standard blu-ray drives
Tif format image files are converted into bianry image by analysis module, using data interaction function when in the corresponding bianry image of click
Arbitrary region when, pixel average size in the region enveloping space can be provided in Sample_intensity display boxes.
The concentration of inserting standard sample and corresponding is separately added into Standard-intensity and Standard-concentration
Image intensity value, testing sample gray value is inserted in Sample-intensity, ' the Fitting Calculation ' is clicked on after being provided with and is pressed
Button can be to provide testing result.
Claims (5)
1. a kind of quantitative detecting method based on the full optical disk image forming system of blu-ray drives, methods described follow these steps to carry out：
In Blu-ray Disc polycarbonate surface biological detection arrays are formed using microflow control technique；
CD is scanned using blu-ray drives, during CD scanning, is adopted using data collecting card from optical head photoelectric detector
Full optical disk image forming system of the data synchronization transmissions collected to standard blu-ray drives on computer；
The data collected are subjected to dimension transformation using the full optical disk image forming system of standard blu-ray drives, feature is alignd, average contracts
Put, full disc image file is formed after coordinate system transformation；
Image analysis module using the full optical disk image forming system of standard blu-ray drives is entered to the pixel value of image file biologic array
Row linear fit, obtain target biological molecules concentration；
Realize imaging analysis and quantitative detection.
2. quantitative detecting method as claimed in claim 1, the quantitative detecting method is to put the CD after biological respinse well,
With metallochrome marker pen in the data of optical disk area for not doing biological respinse, using CD radius as directrix, mark triggers band is as adopting
Collect the characteristic value of data.
3. quantitative detecting method as claimed in claim 1, the quantitative detecting method is will will to gather the files of I 16 of storage
In one-dimensional data sampling process is gradually reduced according to sampling length and sequentially inputs elliptic filter, using LPF, filter
Data after ripple are stored by interception position, and data are 2-D data in the file after storage, realize gathered data dimension transformation,
To the 2-D data after dimension transformation by carrying out Envelope Analysis to single acquisition data, characteristic quantity position is met to width and amplitude
Tagging, and the data for intercepting corresponding length once store so that 2-D data completes feature alignment, and the data after alignment are entered
Every trade row format length, i.e., expanded or compressed in a manner of rounding nearby by setting line number and columns so that data are realized equal
Value scaling.
4. quantitative detecting method as claimed in claim 3, the quantitative detecting method is the full CD in standard blu-ray drives
The image generation module of imaging system average is scaled after 2-D data, by the operation of below equation：
z.jpg
Wherein：M, n are original coordinates；X, y are to meet actual pixels coordinate value；R is imaging region start radius；Δ r is adjacent
The space length of two rows；Δ θ is the angle that adjacent two point data corresponds to space in data line, realizes each data convert
Relevant position onto full disc image.
5. quantitative detecting method as claimed in claim 4, the quantitative detecting method be the full CD of standard blu-ray drives into
As the Analysis of test results module of system, tif format image files are converted into bianry image, then utilize Analysis of test results
The data interaction function of module, when clicking on the arbitrary region in corresponding bianry image, region bag can be provided in display box
Pixel average size in network space, Standard-concentration and Standard- in Analysis of test results module
The concentration for inserting standard sample and respective image pixel value are separately added into pixel value, in Sample-pixel value
In insert testing sample pixel value, ' the Fitting Calculation ' button is clicked on after being provided with can be to provide testing result.
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2017
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| US7170609B2 (en) * | 2003-11-24 | 2007-01-30 | General Electric Company | Sensor systems and methods for quantification of physical parameters, chemical and biochemical volatile and nonvolatile compounds in fluids |
| CN1906489A (en) * | 2003-11-24 | 2007-01-31 | 通用电气公司 | Optical storage medium having an analyte containing polymer film, use thereof |
| CN104991416A (en) * | 2015-07-23 | 2015-10-21 | 太原理工大学 | Optical disc based dimensional periodic micro-nano structure hot padding method |
| CN106442418A (en) * | 2016-09-06 | 2017-02-22 | 大连理工大学 | Low-cost spectroscopic imaging system based on surface plasma resonance |
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