CN107607666A - The detection method of organic acid in a kind of biological sample - Google Patents
The detection method of organic acid in a kind of biological sample Download PDFInfo
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- CN107607666A CN107607666A CN201710949280.4A CN201710949280A CN107607666A CN 107607666 A CN107607666 A CN 107607666A CN 201710949280 A CN201710949280 A CN 201710949280A CN 107607666 A CN107607666 A CN 107607666A
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Abstract
The invention provides a kind of detection method of organic acid in biological sample, and it is to add methanolic to carry out esterification reaction of organic acid again after oximation reaction urina sanguinis or fasting blood, is then detected gained derivative using GC MS.Detection method is accurate, high sensitivity, high specificity, sample loss caused by extraction repeatedly is not only reduced, can also reduce the usage amount of organic solvent, saves the use of urease and methyl-monosilane agent, cost is greatly reduced, and pollution of the waste liquid to environment can be mitigated.
Description
Technical field
The invention belongs to biological sample organic acid analysis field, and in particular to the detection side of organic acid in a kind of biological sample
Method.
Background technology
Metabolism group is the newer research developed rapidly after genomics, transcription group and proteomics
Field, it is the research potential analysis method of Low Molecular Weight Compound in biosystem, it can be used for analyzing biological sample
The change of metabolite level in product, it is to all metabolic components qualitative in the particular organisms sample under qualifications and quantitative.
Fatty acids oxidation group is fatty acid levels and organic acid level in urine in analysis blood.
Gas chromatographyMass spectrometry (gas chromatography-mass spectrometry, GC-MS) in
Inherited Metabolic Disorders detection was initially applied to by K-Tanaka in 1966.The method for being used for urine pretreatment in the world at present mainly has
Two kinds, i.e. organic acid extraction and urease methods, the former is mainly used in American-European countries, and service life is longer, and the latter is near several
A kind of relatively young method that year is established by Japanese scholars and promoted in Asian countries.Urease methods is to collect urine using filter paper
Sample, after urea ferment treatment, GC-MS analyses are directly carried out without organic solvent extraction, but without the gas of organic solvent extraction
The miscellaneous peak of phase chromatogram is a lot of, unfavorable interpretation of result, and very easily pollutes gas chromatographic column and mass ion source.Urine organic acid extracts
Take method for pretreating to be higher than urease pretreatment method to the detected level of various specific metabolic products, and can not be detected for the latter
Aliphatic dicarboxylic acid organic acid through extract method for pretreating remain to detect.At home, the small equality of sieve（2003）With Sun Weihua etc.
（2008）Organic acid in urine is directly extracted using ethyl acetate and ether, and with BSTFA-TMCS（99:1）As derivative
Agent carries out silylation derivative；Korea Spro's wind etc.（2013）Urine sample is first used into urea ferment treatment, removes urea and protein, then
With ethyl acetate and extracted by ether urinary organic acid, then mixed with double (trimethylsilyl) trifluoroacetamides with trim,ethylchlorosilane
Compound carries out silylation derivative.
Continuous development based on mass-spectrometric technique, domestic for organic acid derivatization, this block is also limited to silylation, and
Requirement of the silylation to response sample is anhydrous state, i.e., sample has to nitrogen drying, and this organic acid to extraction will
Ask high, while sample adds the complexity of operation through repeatedly extraction, also easily causes loss.The present invention is surveyed originally using GC
On the basis of determining blood free fatty, extracted after directly esterified to organic acid/fatty acid methyl in a system, not only
Reduce sample loss caused by extraction possibility repeatedly, moreover it is possible to meet the detection of metabolin organic acid omega-dicarboxylic acids well, and
Both at home and abroad there is not yet by relevant report.
The content of the invention
It is an object of the invention to provide a kind of detection method of organic acid in biological sample, this not only reduces extract repeatedly
Sample loss caused by taking, the usage amount of organic solvent can be also reduced, save the use of urease and methyl-monosilane agent, significantly
Cost is reduced, and pollution of the waste liquid to environment can be mitigated.
To achieve the above object, the present invention adopts the following technical scheme that：
The detection method of organic acid in a kind of biological sample, it comprises the following steps：
1）Urina sanguinis or fasting blood are centrifuged into 8min in 2200g, takes supernatant to be sub-packed in cryopreservation tube, is saved backup in -20 DEG C；
2）Gained supernatant 1mL is taken to add 100 μ L internal standard compound Heptadecanoic acides in centrifuge tube, shake up, add 100 μ L mass
Concentration is 2.5% hydroxylamine hydrochloride and 300 μ L, 10mol/L sodium hydroxide solution, is covered tightly, and 1h is stood after fully mixing, then add
Enter the sulfuric acid that 130 μ L mass concentrations are 50% to be acidified, be eventually adding the methanolic solution that 2mL mass concentrations are 10%, whirlpool shakes
1min is swung after esterification reaction of organic acid 90min in 60 DEG C of water-baths；
3）Reaction is drawn off after terminating, and is put to room temperature, is then added 1mL ultra-pure waters and is mixed, adds 2mL chloroforms, whirlpool shakes
Swing 2min and centrifuge 8min after 2200g, obtain lower floor's solution；
4）Gained lower floor solution using GC-MS through in 0.45 μm of rearmounted sample bottle of the organic membrane filtration of micropore, being detected；Its color
Spectral condition is：Hp-5ms chromatographic columns（30 m × 0.25 mm, 0.25 μm）；Sample size：1μL；Heating schedule：Initial 40 DEG C of holdings
3min, 290 DEG C then are warming up to 8 DEG C/min, keep 3min；Using helium as carrier gas；Flow velocity is 1.0mL/min；Injection port temperature
Degree：260 DEG C, transmission line：290℃；Using electron impact ion source Mass Spectrometer Method, its electron energy 70 eV；Ion source temperature
230℃；Scanning range：M/z 50-550, scan mode：Full scan and selection ion scan, solvent delay 3.0min.
The remarkable advantage of the present invention is：
The present invention can be achieved to detect while a variety of organic acids, and sample loss caused by this not only reduces extracting repeatedly, also
The usage amount of organic solvent can be reduced, eliminates the use of urease and methyl-monosilane agent, greatly reduces cost, and can subtract
Light pollution of the waste liquid to environment.
Embodiment
In order that content of the present invention easily facilitates understanding, with reference to embodiment to of the present invention
Technical scheme is described further, but the present invention is not limited only to this.
Agilent 7890A/5975C gas chromatograph-mass spectrometers, equipped with electron impact ion source（EI）And chem workstation
（Agilent companies of the U.S.）.
The detection method of organic acid in a kind of biological sample, it comprises the following steps：
1）Urina sanguinis or fasting blood are centrifuged into 8min in 2200g, takes supernatant to be sub-packed in cryopreservation tube, is saved backup in -20 DEG C；
2）Gained supernatant 1mL is taken to add 100 μ L internal standard compound Heptadecanoic acides in centrifuge tube, shake up, add 100 μ L mass
Concentration is 2.5% hydroxylamine hydrochloride and 300 μ L, 10mol/L sodium hydroxide solution, is covered tightly, and 1h is stood after fully mixing, then add
Enter the sulfuric acid that 130 μ L mass concentrations are 50% to be acidified, be eventually adding the methanolic solution that 2mL mass concentrations are 10%, whirlpool shakes
1min is swung after esterification reaction of organic acid 90min in 60 DEG C of water-baths；
3）Reaction is drawn off after terminating, and is put to room temperature, is then added 1mL ultra-pure waters and is mixed, adds 2mL chloroforms, whirlpool shakes
Swing 2min and centrifuge 8min after 2200g, obtain lower floor's solution；
4）By gained lower floor solution through in 0.45 μm of rearmounted sample bottle of the organic membrane filtration of micropore, being detected using GC-MS；Its
Chromatographic condition is：Hp-5ms chromatographic columns（30 m × 0.25 mm, 0.25 μm）；Sample size：1μL；Heating schedule：Initial 40 DEG C of guarantors
3min is held, is then warming up to 290 DEG C with 8 DEG C/min, keeps 3min；Using helium as carrier gas；Flow velocity is 1.0mL/min；Injection port
Temperature：260 DEG C, transmission line：290℃；Using electron impact ion source Mass Spectrometer Method, its electron energy 70 eV；Ion source temperature
230℃；Scanning range：M/z 50-550, scan mode：Full scan and selection ion scan, solvent delay 3.0min.
1. standard working curve is prepared：Accurately weigh laurate（Dode A）, myristic acid（Tetr A）, suberic acid
（Sub A）, pimelic acid（Hept A）, adipic acid（Adip A）, azelaic acid（Azel A）, methylmalonic acid（Mm A）, isovaleric acid
（Isov A）, palmitic acid（Hex A）Each 10.0mg of reference substance, it is configured to the reference substance that concentration is 1.0mg/mL with methanol solution and stores up
Standby liquid A；Take liquid r- leukotrienes（r-LNA）, arachidonic acid（AA）, eicosapentaenoic acid（EPA）, docosahexaenoic acid
（DHA）Each 10mg of reference substance, the reference substance storing solution B for being 1.0mg/mL into concentration with n-hexane accurate formulation.Reference substance is stored up
Standby liquid A and B obtain a series of reference substance solution of various concentrations after being diluted in various degree with methanol and n-hexane respectively.By gained
After being handled by reference substance solution by the inventive method, using reference substance and the peak area ratio of internal standard substance as ordinate, with
Its concentration is abscissa, linear recurrence, and gained regression equation is shown in Table 1.
The linear regression of table 1
From table 1, linearly dependent coefficient R >=0.99 of each reference substance, show that linear relationship is good.
Detected through GC-MS, retention time, characteristic ion and the monitoring time of each reference substance and internal standard esterification are shown in Table 2.
2 each reference substance of the table and retention time of internal standard esterification, characteristic ion and monitoring time
Therefore, by monitoring feature ion and combination chromatographic peak retention time and abundance of ions ratio, qualitative analysis can be carried out, and can
According to analyte and the area ratio of internal standard chromatographic peak, quantitative analysis is carried out with calibration curve method.
2. Method validation：Be separately added into healthy person urine sample 1.0 μ g/mL, 10.0 μ g/mL, 2 concentration contain 4
Kind organic acid（Adip A、Hept A、Sub A、Azel A）Mixed standard solution, according to the inventive method Treatment Analysis, carry out
Methodology detects, as a result as shown in table 3.Each sample parallel analysis 4 times.
The precision of table 3 and recovery test
From table 3, the inventive method is stable, and the degree of accuracy is high.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, it should all belong to the covering scope of the present invention.
Claims (3)
- A kind of 1. detection method of organic acid in biological sample, it is characterised in that：Comprise the following steps：1）Urina sanguinis or fasting blood are centrifuged into 8min in 2200g, take supernatant standby；2）Take step 1）Gained supernatant 1mL adds 100 μ L internal standard compounds, shakes up, it is dense to add 100 μ L mass in centrifuge tube Spend for 2.5% hydroxylamine hydrochloride and 300 μ L, 10mol/L sodium hydroxide solution, cover tightly, stand 1h after fully mixing, add The sulfuric acid that 130 μ L mass concentrations are 50% is acidified, and is eventually adding the methanolic solution that 2mL mass concentrations are 10%, vortex oscillation 1min is after esterification reaction of organic acid 90min in 60 DEG C of water-baths；3）Reaction is drawn off after terminating, and is put to room temperature, is then added 1mL ultra-pure waters and is mixed, adds 2mL chloroforms, whirlpool shakes Swing 2min and centrifuge 8min after 2200g, remove a layer solution；4）By gained lower floor solution after 0.45 μm of organic membrane filtration of micropore, detected using GC-MS.
- 2. according to claim 1 in biological sample organic acid detection method, it is characterised in that：Step 2）Described in internal standard Thing is Heptadecanoic acide.
- 3. according to claim 1 in biological sample organic acid detection method, it is characterised in that：Color when GC-MS is detected Spectral condition is：Hp-5ms chromatographic columns, 30 m × 0.25 mm, 0.25 μm；Sample size：1μL；Heating schedule：Initial 40 DEG C of holdings 3min, 290 DEG C then are warming up to 8 DEG C/min, keep 3min；Using helium as carrier gas；Flow velocity is 1.0mL/min；Injection port temperature Degree：260 DEG C, transmission line：290℃；Using electron impact ion source Mass Spectrometer Method, its electron energy 70 eV；Ion source temperature 230℃；Scanning range：M/z 50-550, scan mode：Full scan and selection ion scan, solvent delay 3.0min.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108414651A (en) * | 2018-02-09 | 2018-08-17 | 深圳爱湾医学检验实验室 | The a variety of organic acid assay kits of derivatization and its application method |
| CN108535395A (en) * | 2018-03-12 | 2018-09-14 | 安徽古井贡酒股份有限公司 | A method of using 32 kinds of free fatties in UPLC-QTof Rapid Simultaneous Determination health liquors |
| CN109406690A (en) * | 2018-11-25 | 2019-03-01 | 山东达因海洋生物制药股份有限公司 | A kind of method in relation to substance in detection chloraldurate |
| CN111289646A (en) * | 2020-03-06 | 2020-06-16 | 贵州省烟草科学研究院 | Qualitative and quantitative method for organic acid substances in biodegradable mulching film |
| CN111406214A (en) * | 2018-01-10 | 2020-07-10 | 弗门尼舍有限公司 | Suppression of sweat malodour |
| CN113176372A (en) * | 2021-05-20 | 2021-07-27 | 北京化工大学 | Gas chromatography method for detecting content of adipic acid in fermentation liquor |
| CN113777188A (en) * | 2021-08-26 | 2021-12-10 | 深圳爱湾医学检验实验室 | Glutaric aciduria type 1 detection device, urine organic acid content detection method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102621247A (en) * | 2012-04-18 | 2012-08-01 | 王益超 | Method for synchronously analyzing base, nucleotide, organic acid, fatty acid, amino acid and saccharide metabolic product with two-step derivation method |
| CN102621262A (en) * | 2012-02-28 | 2012-08-01 | 杭州博圣生物技术有限公司 | Method for detecting organic acid in urine by means of gas chromatography and mass spectrography |
| CN102621249A (en) * | 2012-04-18 | 2012-08-01 | 王益超 | Method for synchronously analyzing base, nucleotide, organic acid, fatty acid, amino acid and saccharide metabolic product with multi-step derivation method |
| CN103837634A (en) * | 2014-03-21 | 2014-06-04 | 上海硕源健标生物医学科技有限公司 | Method for simultaneously detecting contents of various organic acids in urine of human body |
| CN105954454A (en) * | 2016-07-14 | 2016-09-21 | 云南中烟工业有限责任公司 | Method for separating and assaying organic acid and fatty acid substances in saliva |
-
2017
- 2017-10-12 CN CN201710949280.4A patent/CN107607666A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102621262A (en) * | 2012-02-28 | 2012-08-01 | 杭州博圣生物技术有限公司 | Method for detecting organic acid in urine by means of gas chromatography and mass spectrography |
| CN102621247A (en) * | 2012-04-18 | 2012-08-01 | 王益超 | Method for synchronously analyzing base, nucleotide, organic acid, fatty acid, amino acid and saccharide metabolic product with two-step derivation method |
| CN102621249A (en) * | 2012-04-18 | 2012-08-01 | 王益超 | Method for synchronously analyzing base, nucleotide, organic acid, fatty acid, amino acid and saccharide metabolic product with multi-step derivation method |
| CN103837634A (en) * | 2014-03-21 | 2014-06-04 | 上海硕源健标生物医学科技有限公司 | Method for simultaneously detecting contents of various organic acids in urine of human body |
| CN105954454A (en) * | 2016-07-14 | 2016-09-21 | 云南中烟工业有限责任公司 | Method for separating and assaying organic acid and fatty acid substances in saliva |
Non-Patent Citations (1)
| Title |
|---|
| 马晓丽 等: "基于气相色谱的血清中游离脂肪酸代谢轮廓分析区分糖尿病与正常人", 《分析科学学报》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111406214A (en) * | 2018-01-10 | 2020-07-10 | 弗门尼舍有限公司 | Suppression of sweat malodour |
| CN108414651A (en) * | 2018-02-09 | 2018-08-17 | 深圳爱湾医学检验实验室 | The a variety of organic acid assay kits of derivatization and its application method |
| CN108535395A (en) * | 2018-03-12 | 2018-09-14 | 安徽古井贡酒股份有限公司 | A method of using 32 kinds of free fatties in UPLC-QTof Rapid Simultaneous Determination health liquors |
| CN108535395B (en) * | 2018-03-12 | 2020-01-31 | 安徽古井贡酒股份有限公司 | method for simultaneously and rapidly measuring 32 free fatty acids in health-care wine |
| CN109406690A (en) * | 2018-11-25 | 2019-03-01 | 山东达因海洋生物制药股份有限公司 | A kind of method in relation to substance in detection chloraldurate |
| CN109406690B (en) * | 2018-11-25 | 2022-02-25 | 山东达因海洋生物制药股份有限公司 | Method for detecting related substances in chloral hydrate |
| CN111289646A (en) * | 2020-03-06 | 2020-06-16 | 贵州省烟草科学研究院 | Qualitative and quantitative method for organic acid substances in biodegradable mulching film |
| CN111289646B (en) * | 2020-03-06 | 2022-11-04 | 贵州省烟草科学研究院 | Qualitative and quantitative method for organic acid substances in biodegradable mulching film |
| CN113176372A (en) * | 2021-05-20 | 2021-07-27 | 北京化工大学 | Gas chromatography method for detecting content of adipic acid in fermentation liquor |
| CN113777188A (en) * | 2021-08-26 | 2021-12-10 | 深圳爱湾医学检验实验室 | Glutaric aciduria type 1 detection device, urine organic acid content detection method and application thereof |
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