CN107607529A - A kind of quick screening method for being used for formaldehyde in perfume - Google Patents
A kind of quick screening method for being used for formaldehyde in perfume Download PDFInfo
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- CN107607529A CN107607529A CN201710709774.5A CN201710709774A CN107607529A CN 107607529 A CN107607529 A CN 107607529A CN 201710709774 A CN201710709774 A CN 201710709774A CN 107607529 A CN107607529 A CN 107607529A
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Abstract
The invention discloses a kind of quick screening method for being used for formaldehyde in perfume.This method comprises the following steps：1) fast screening reagent is made：By phloroglucin, sodium hydroxide and sodium sulphate mixing, fast screening reagent is made；2) color scale card is made：Formaldehyde standard liquid is prepared, takes formaldehyde standard liquid, in being mixed in colorimetric cylinder, the color change of recording solution, color scale card to be made with fast screening reagent；3) detect：Take perfume sampler, in being mixed in colorimetric cylinder, to record the color change of sample solution with fast screening reagent, contrasted with color scale card, qualitative analysis is carried out to the formaldehyde in perfume sampler.The present invention is used for the quick screening method of formaldehyde in perfume, and its high sensitivity, the degree of accuracy is high, simple to operate, detection time is short, it is not necessary to Special Equipment, is adapted to execute-in-place, is especially suitable for superintendent office, testing agency, the demand of all kinds of mechanism field quick detections.
Description
Technical field
The present invention relates to a kind of quick screening method for being used for formaldehyde in perfume.
Background technology
Formaldehyde due to its good hydrophilic property, sterilizing rate is high, cheap and the acid-base value in by system is not influenceed the advantages that,
And it is commonly used for the preservative of cosmetics.A kind of potential health risk material of the formaldehyde as everyday exposure, it is to health and ring
The influence in border be can not be ignored, and for this, the public is extremely paid close attention to it.
Formaldehyde is used as compared with high toxic material, second is on China's toxic chemical priority acccess control list, by generation
Boundary's health organization is defined as carcinogenic and causes deformed material, is generally acknowledged allergen, and one of potential strong mutagen.
It is formic acid, carbon dioxide etc. that formaldehyde mainly enters human body and tachymetabolism by respiratory tract.Formaldehyde and its metabolin also with ammonia
Base acid, protein, nucleic acid etc. form unstable compound, are transferred to kidney, liver and hematopoietic tissue and play a role, to each system of human body
System has different degrees of harm.
Formaldehyde sucks the electrophysiological function that can influence heart, causes the rhythm of the heart too slow.Under certain pathological conditions, formaldehyde can
Interacted with peroxide, arteries and veins is activated by promoting the formation of LDL oxidation, platelet aggregation and thromboxane
The formation of atherosis, cause the murder by poisoning to cardiovascular system.Formaldehyde has strong impulse effect to skin and mucous membrane, connects repeatedly
Tactile formalin can cause allergic dermatitis, color spot.Formaldehyde also has heredity damage to bronchial epithelial cell, pulmonary macrophage
Wound.Due to the water solubility of height, formaldehyde is easily rich in moisture mucus in nose, nasal sinus and trachea-bronchial epithelial cell mucous membrane absorbs,
And combine with protein therein, polysaccharide material, destroy the transport mechanism of mucus cilia, show obvious local excitation disease
Shape.
China《Cosmetics safety technical specification》Clear stipulaties formaldehyde is disabling thing in spray product in (version in 2015)
Matter, and define the health chemical testing method of Formaldehyde In Cosmetics in chapter 4.At present, Formaldehyde In Cosmetics detection is usual
Analyzed using liquid chromatogram, cumbersome, Check-Out Time length, be not suitable for superintendent office, testing agency, all kinds of mechanisms show
The requirement of field quick detection.
The content of the invention
It is an object of the invention to provide a kind of quick screening method for being used for formaldehyde in perfume.
The technical solution used in the present invention is：
A kind of quick screening method for being used for formaldehyde in perfume, comprises the following steps：
1) fast screening reagent is made：By phloroglucin, sodium hydroxide and sodium sulphate mixing, fast screening reagent is made；
2) color scale card is made：Formaldehyde standard liquid is prepared, takes formaldehyde standard liquid and fast screening reagent in being mixed in colorimetric cylinder,
The color change of recording solution, is made color scale card；
3) detect：Perfume sampler is taken, in being mixed in colorimetric cylinder, the color change of sample solution to be recorded, with color with fast screening reagent
Rank card is contrasted, and qualitative analysis is carried out to the formaldehyde in perfume sampler.
In step 1), phloroglucin, the mass ratio of sodium hydroxide and sodium sulphate is (2~5)：(25~100)：100.
In step 2), the concentration range of formaldehyde standard liquid is (0~500) μ g/mL.
In step 2), the amount ratio of formaldehyde standard liquid and fast screening reagent is 1mL：(25~50) mg.
In step 2), the time of recording solution color change is after formaldehyde standard liquid contacts mixing with fast screening reagent
1min。
In step 3), samples of perfume and the amount ratio of fast screening reagent are 1mL：(25~50) mg.
In step 3), the time for recording sample solution color change is specific at twice：First record be sample solution with
1min after fast screening reagent contact mixing, is recorded as sample solution and 10~15min after mixing is contacted with fast screening reagent for the second time.
In step 3), qualitative analysis is specially：If sample solution do not present it is orange or orange red, or during first record
Present orange or orange red and colour-fast when second records, be then determined as feminine gender；If sample solution is in first record
To be orange or orange red, and lighter or taken off when second records, be then determined as the positive.
The beneficial effects of the invention are as follows：
The present invention is used for the quick screening method of formaldehyde in perfume, and its high sensitivity, the degree of accuracy is high, simple to operate, detection
Time is short, it is not necessary to Special Equipment, is adapted to execute-in-place, is especially suitable for superintendent office, testing agency, all kinds of mechanisms scene quickly
The demand of detection.
It is specific as follows：
For the present invention without carrying out any pre-treatment to sample, fast screening reagent need not also be configured to solution, directly by sample with
Fast screening reagent mixing quickly can have in judgement sample formaldehydeless by front and rear color change.Because water content is very low in perfume
(main component of perfume is ethanol and essence and flavoring agent), therefore the present invention only needs less moisture can whether there is first in judgement sample
Aldehyde, suitable for the low fluid sample of water content.Method is simple to operate, detection time is short, it is not necessary to Special Equipment, is adapted to scene behaviour
Make, suitable for the requirement of superintendent office, testing agency, all kinds of mechanism field quick detections.
Embodiment
A kind of quick screening method for being used for formaldehyde in perfume, comprises the following steps：
1) fast screening reagent is made：By phloroglucin, sodium hydroxide and sodium sulphate mixing, fast screening reagent is made；
2) color scale card is made：Formaldehyde standard liquid is prepared, takes formaldehyde standard liquid and fast screening reagent in being mixed in colorimetric cylinder,
The color change of recording solution, is made color scale card；
3) detect：Perfume sampler is taken, in being mixed in colorimetric cylinder, the color change of sample solution to be recorded, with color with fast screening reagent
Rank card is contrasted, and qualitative analysis is carried out to the formaldehyde in perfume sampler.
Preferably, in step 1), phloroglucin, the mass ratio of sodium hydroxide and sodium sulphate is (2~5)：(25~100)：
100.Described sodium sulphate is anhydrous sodium sulfate.
Preferably, in step 2), formalin is diluted into compounding methanol standard liquid, wherein ethanol with ethanol water
The volumetric concentration of the aqueous solution is 75%.
Preferably, in step 2), the concentration range of formaldehyde standard liquid is (0~500) μ g/mL.
Preferably, in step 2), the amount ratio of formaldehyde standard liquid and fast screening reagent is 1mL：(25~50) mg.
Preferably, in step 2), the time of recording solution color change contacts for formaldehyde standard liquid with fast screening reagent mixes
1min after conjunction.
Preferably, in step 3), samples of perfume and the amount ratio of fast screening reagent are 1mL：(25~50) mg.
Preferably, in step 3), the time for recording sample solution color change is specific at twice：First record is sample
Solution contacted with fast screening reagent mixing after 1min, be recorded as the second time sample solution contacted with fast screening reagent mix after 10~
15min。
In step 3), qualitative analysis is specially：If sample solution do not present it is orange or orange red, or during first record
Present orange or orange red and colour-fast when second records, be then determined as feminine gender；If sample solution is in first record
To be orange or orange red, and lighter or taken off when second records, be then determined as the positive.
The present invention colour developing principle be：Using the chemical feature of formaldehyde, make itself and phloroglucinol reaction, then with sodium hydroxide
Act on and develop the color, using heat caused by sodium hydrate solid and water release chemical energy, accelerate chemical reaction rate, chromogenic reaction
Substantially.
Present disclosure is described in further detail below by way of specific embodiment.
Embodiment 1：
A kind of method for being used for formaldehyde rapid screening in perfume, comprises the following steps：
(1) phloroglucin 80mg, sodium hydroxide 1g are weighed, anhydrous sodium sulfate 2g is distributed into ratio in reagent bottle, after mixing
In colour tube, every pipe 50mg.
(2) formalin standard substance is diluted to 0,5,10,25,50,100 μ g/ with 75% (v/v) ethanol water
ML standard liquid.
Above-mentioned standard solution 2mL is taken to make solution and reagent in simultaneously timing in the colorimetric cylinder equipped with fast screening reagent, vibration respectively
Fully contact, observe during 1min and produce orange red compound, concentration is higher, and color is deeper, photographs to record color, makes quality
Rank is blocked.
(3) take 2mL samples in the colorimetric cylinder equipped with fast screening reagent and timing, vibration make solution fully be contacted with reagent,
Observation color has unchanged, and color change is photographed to record respectively at 1min, 15min, so as to whether contain formaldehyde in judgement sample.
Embodiment 2：
A kind of method for being used for formaldehyde rapid screening in perfume, comprises the following steps：
(1) phloroglucin 40mg, sodium hydroxide 2g are weighed, anhydrous sodium sulfate 2g is distributed into ratio in reagent bottle, after mixing
In colour tube, every pipe 50mg.
(2) formalin standard substance is diluted to 0,5,10,25,50,100 μ g/ with 75% (v/v) ethanol water
ML standard liquid.
Above-mentioned standard solution 2mL is taken to make solution and reagent in simultaneously timing in the colorimetric cylinder equipped with fast screening reagent, vibration respectively
Fully contact, observe during 1min and produce orange red compound, concentration is higher, and color is deeper, photographs to record color, makes quality
Rank is blocked.
(3) take 2mL samples in the colorimetric cylinder equipped with fast screening reagent and timing, vibration make solution fully be contacted with reagent,
Observation color has unchanged, and color change is photographed to record respectively at 1min, 10min, so as to whether contain formaldehyde in judgement sample.
Embodiment 3：
A kind of method for being used for formaldehyde rapid screening in perfume, comprises the following steps：
(1) phloroglucin 100mg, sodium hydroxide 2g are weighed, anhydrous sodium sulfate 2g is distributed into ratio in reagent bottle, after mixing
In colour tube, every pipe 100mg.
(2) formalin standard substance is diluted to 50,100,250,500 μ g/mL with 75% (v/v) ethanol water
Standard liquid.
Above-mentioned standard solution 2mL is taken to make solution and reagent in simultaneously timing in the colorimetric cylinder equipped with fast screening reagent, vibration respectively
Fully contact, observe during 1min and produce orange red compound, concentration is higher, and color is deeper, photographs to record color, makes quality
Rank is blocked.
(3) take 2mL samples in the colorimetric cylinder equipped with fast screening reagent and timing, vibration make solution fully be contacted with reagent,
Observation color has unchanged, and color change is photographed to record respectively at 1min, 15min, so as to whether contain formaldehyde in judgement sample.
Claims (8)
- A kind of 1. quick screening method for being used for formaldehyde in perfume, it is characterised in that：Comprise the following steps：1) fast screening reagent is made：By phloroglucin, sodium hydroxide and sodium sulphate mixing, fast screening reagent is made；2) color scale card is made：Formaldehyde standard liquid is prepared, takes formaldehyde standard liquid, in being mixed in colorimetric cylinder, to be recorded with fast screening reagent The color change of solution, is made color scale card；3) detect：Perfume sampler is taken, in being mixed in colorimetric cylinder, the color change of sample solution to be recorded, with color scale card with fast screening reagent Contrasted, qualitative analysis is carried out to the formaldehyde in perfume sampler.
- A kind of 2. quick screening method for being used for formaldehyde in perfume according to claim 1, it is characterised in that：Step 1) In, phloroglucin, the mass ratio of sodium hydroxide and sodium sulphate is (2~5)：(25~100)：100.
- A kind of 3. quick screening method for being used for formaldehyde in perfume according to claim 1, it is characterised in that：Step 2) In, the concentration range of formaldehyde standard liquid is (0~500) μ g/mL.
- A kind of 4. quick screening method for being used for formaldehyde in perfume according to claim 1, it is characterised in that：Step 2) In, the amount ratio of formaldehyde standard liquid and fast screening reagent is 1mL：(25~50) mg.
- A kind of 5. quick screening method for being used for formaldehyde in perfume according to claim 1, it is characterised in that：Step 2) In, the time of recording solution color change is that formaldehyde standard liquid contacts 1min after mixing with fast screening reagent.
- A kind of 6. quick screening method for being used for formaldehyde in perfume according to claim 1, it is characterised in that：Step 3) In, samples of perfume and the amount ratio of fast screening reagent are 1mL：(25~50) mg.
- A kind of 7. quick screening method for being used for formaldehyde in perfume according to claim 1, it is characterised in that：Step 3) In, the time for recording sample solution color change is specific at twice：First record contacts mixed for sample solution with fast screening reagent 1min after conjunction, sample solution is recorded as the second time 10~15min after mixing is contacted with fast screening reagent.
- A kind of 8. quick screening method for being used for formaldehyde in perfume according to claim 7, it is characterised in that：Step 3) In, qualitative analysis is specially：If sample solution does not present orange or orange red, or presented during first record orange or orange red Color and colour-fast when second records, then be determined as feminine gender；If sample solution is orange or orange red in first record, And lighter or taken off when second records, then it is determined as the positive.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710709774.5A CN107607529B (en) | 2017-08-18 | 2017-08-18 | Rapid screening method for formaldehyde in perfume |
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| CN201710709774.5A CN107607529B (en) | 2017-08-18 | 2017-08-18 | Rapid screening method for formaldehyde in perfume |
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| CN107607529B CN107607529B (en) | 2019-12-06 |
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| CN201710709774.5A Active CN107607529B (en) | 2017-08-18 | 2017-08-18 | Rapid screening method for formaldehyde in perfume |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110118770A (en) * | 2019-04-19 | 2019-08-13 | 广州安诺科技股份有限公司 | It is a kind of for detecting the reagent of sodium formaldehyde sulfoxylate, kit and production technology in food |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110118770A (en) * | 2019-04-19 | 2019-08-13 | 广州安诺科技股份有限公司 | It is a kind of for detecting the reagent of sodium formaldehyde sulfoxylate, kit and production technology in food |
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| CN107607529B (en) | 2019-12-06 |
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