CN107603904A - A kind of streptomycete and its application - Google Patents
A kind of streptomycete and its application Download PDFInfo
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- CN107603904A CN107603904A CN201710719891.XA CN201710719891A CN107603904A CN 107603904 A CN107603904 A CN 107603904A CN 201710719891 A CN201710719891 A CN 201710719891A CN 107603904 A CN107603904 A CN 107603904A
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- streptomycete
- streptomyces
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Abstract
The present invention relates to a kind of streptomycete and its application, belongs to biological technical field.The streptomycete is obtained by production bacterial strain and auxiliary material after primary dcreening operation, secondary screening, natural medium culture and streptomycete purify culture processes, production bacterial strain is the S of streptomycete Streptomyces sp.B 3, China typical culture collection center, preserving number are deposited on June 26th, 2017：CCTCC No.M2017378.The streptomycete, which has, promotes large leaf tea growth, strengthens the effect of its disease resistance, in the cultivation applied to large leaf tea tree；The S of B 3 are used in the cultivation of large leaf tea tree by the present invention according to a certain percentage, and the growth of tea tree is vigorous, disease resistance enhancing, are remarkably improved yield, reduce the use of chemical pesticide and chemical fertilizer, stabilizing and increasing for tea leaf quality is played a significant role.
Description
Technical field
The present invention relates to biological technical field, is a kind of streptomycete and its application in particular.
Background technology
Endophytic actinomycetes in plants (Endophytic actinomycetes) is found in plant health organization internal.Plant
Thing endogeny rayungus is a kind of important microbial resources, can produce a variety of farm antibiotics and plant growth regulator, can have
Effect ground preventing and treating host plant disease, improve the yield and quality of plant product.
In Elevation cultivation, the endogenetic streptomycete can secrete antimycotic material and act on plant host, side
Host plant disease-resistant fungal pathogen sickle-like bacteria (Fusarium) is helped, intends disk stey (Pestalotiopsis) and tea moire leaf is withered
The infection of sick pathogen (Colletotrichum camelliae), significantly improve the disease resistance of plant, while the bacterial strain tieback
The Net Photosynthetic Rate of plant, transpiration rate, stomatal conductance are remarkably improved in plant host, reduces intercellular CO2Concentration reduces,
Promote plant growth.Bio-bacterial manure is being made using the bacterial strain, the use of chemical pesticide and chemical fertilizer can reduced, to Pu'er tea product
The raising of matter has positive facilitation.
In Elevation endophyte research field, especially long-term lacking more systematic research, endogeny rayungus
Diversity and bioactivity research.And how by studying Elevation endogeny rayungus, and therefrom sieve
Select beneficial microbe strain, using with antimycotic, the streptomycete bacterial strain of growth promoting function cultivated to large leaf tea in lifting
Tealeaves and yield and quality, there is not yet disclosed document report.
The content of the invention
It is an object of the invention to bio-bacterial manure is studied from the angle of tea tree endosymbiontic microorganism, there is provided a kind of
Streptomycete bacterial strain and its application with antimycotic growth promoting function, to promote Elevation cultivation, improve Pu'er tea product
Matter plays a role.
The streptomycete of the present invention, trained by production bacterial strain and auxiliary material through primary dcreening operation, secondary screening, natural medium and synthetic media
Obtained after foster and streptomycete purifying culture processes, streptomycete Streptomyces sp.B-3-S were in preservation on June 26 in 2017
In China typical culture collection center, depositary institution address：Wuchang, wuhan area Bayi Road Luo Jia Shan, China of Wuhan University
Type Tissue Collection.Preserving number：CCTCC No.M2017378.
Streptomycete (Streptomyces) B-3-S morphological feature of the present invention is：
Isolated strains have flourishing gas silk and base silk on Gause I culture medium.Gas silk canescence, base silk are light yellow.
5-7 days diameters of the mushroom bacterium colony can reach 3-4mm, and mycelia is just white, rear light grey, villiform, reverse side often occur
Yellowish.Observation is continuous without tabula with flourishing hyperbranched substrate mycelium and aerial hyphae, mycelia under the microscope
Split, spore chain prosperity is in the shape of a spiral, verticillate.Spore circle or oval.
Primary dcreening operation, secondary screening, natural medium and synthetic media culture, streptomycete purifying culture pass known to present invention warp
Obtained after system process.
Streptomycete (Streptomyces) B-3-S actinomyces of the present invention are improved tea tree disease resistance, promote growth of tea plant
Effect, applied to Elevation cultivate in.
Streptomycete (Streptomyces sp.) B-3-S described in claim 1 is applied to the life of crops bio-bacterial manure
In production. art
The advantage of the invention is that：Bacterial manure is made in B-3-S and is used for Elevation cultivation according to a certain percentage
In, the disease resistance of tea tree is remarkably improved, and photosynthetic efficiency is remarkably reinforced, and can substantially reduce chemical pesticide and chemical fertilizer
Usage amount, to reduce agriculture it is residual, the quality for improving tealeaves plays a significant role.
Embodiment
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, below
In conjunction with specific embodiments, the present invention is expanded on further.
Embodiment 1：B-3-S bacteria selections
From the tea tree sample collected, (culture medium with TWYE culture mediums) is separated by known flat board and obtains 15
Endogenetic streptomycete bacterial strain.Endogenetic streptomycete pure medium is：Yeast extract 0.25g, K2HPO40.5g, agar 15g, distilled water
1000ml, pH value 7.2, sterilize 30min at 121 DEG C.Filter out to sickle-like bacteria (Fusarium) by filter paper enzyme again and intend disk
Stey (Pestalotiopsis) and tea moire blight pathogen (Colletotrichum camelliae), which have, to be suppressed
Active while active No. 3 stronger bacterial strains are as test organisms.Screening technique is as follows：
A. primary dcreening operation：By the streptomycete bacterial strain being separated to be inoculated into Gause I fluid nutrient medium (soluble starch 20g,
KNO31g, NaCl 0.5g, K2HPO40.5g, MgSO40.5g, FeSO40.01g, distilled water 1000ml, pH 7.2-7.4,
Sterilize 30min in 121 DEG C of pressure cookers) on, cultivated seven days in 28 DEG C.Separating payingoff bacteria bacterial strain group DNA is extracted, using merger
Primer pair amplifies PKS- I KS genetic fragments, PKS- II ACP genes and NRPS genes, obtain genescreen positive strain.
Principle be most of actinomyces caused by secondary metabolite be by polyketide synthases route of synthesis (PKS) and
Nonribosomal Peptide Synthetases (NRPS) approach is formed.Have in the medical or farm antibiotics listed at present it is a variety of belong to such,
Although these Antibiotique composition structural differences are very big, its biosynthesis pathway is as regular as clockwork, and its synthetic gene has one
Fixed homology, therefore can be by using specific gene as selection markers.Select PKS, NRPS gene PCR product difference larger,
The stronger bacterial strain of antagonistic activity is further to be studied.
B. secondary screening：With the bottled 50ml culture mediums of 250ml triangles (soluble starch 20g, KNO31g, NaCl 0.5g,
K2HPO40.5g, MgSO40.5g, FeSO40.01g, distilled water 1000ml pH 7.2-7.4) sterilizing after access primary dcreening operation chain
Mold species, in shake flask culture 7 days (28 DEG C).Then strain growth situation is determined with nephelometer.Every plant of bacterium inoculation three
Bottle.One plant of most vigorous bacterial strain of growth is finally selected as experimental strain.
C. the strain selected finally is inoculated on natural medium (tryptone 15g, soy peptone 5g, NaCl
5g, add distilled water to 1000mL, pH value 7.2).After being put into the shake culture 5~7 days of 28 DEG C of constant-temperature table (150rpm/min),
It is sterile it is super make to be filtered with aseptic filter paper on platform, the spore of mycelia and streptomycete on sterile harvest filter paper, be put into 28 DEG C of insulating boxs
Middle drying 24 hours, inoculation fermentation experiment can be used for.
Embodiment 2：B-3-S production application
Large leaf tea tea tree seedling is cultivated by traditional method, weight ratio is then inoculated with cultivation matrix is
It after being inoculated with 14 days, can be found from seedling root infection plant from the root, stem, leaf texture of plant by 0.1-0.8% B-3-S
B-3-S is colonized, and endosymbiosis relation is established with plant；And with the increase of B-3-S quantity, it is living to secret out of substantial amounts of biology
Property material, the influence to plant growth are gradually obvious.These bioactive substances are remarkably improved the photosynthesis effect of tea tree
Rate, makes the increment of tea tree substantially increase.The resistance rate enhancing to venereal infection fungal pathogenses, disease plant significantly reduce simultaneously,
This illustrates, its growth-promoting functions and disease resistance are significantly improved in tieback B-3-S to tea tree tissue, and chemical pesticide and change
The use reduction of fertilizer is residual for reducing agriculture, and reducing the influence to soil microflora all has clear meaning.But due to dew
In the tea tree of ground plantation in addition to B-3-S, there are substantial amounts of other endosymbiontic microorganisms to start to grow, raw microorganism can in advantage
It can be changed with plant growing condition, it is therefore desirable to four months periodically inoculation endogenetic streptomycete bacterial strains are spaced, to ensure its hair
The effect of waving.
Large leaf tea tree after being inoculated with B-3-S, its growth quality are significantly improved, and outer viewing plant is more healthy and stronger,
Blade is larger, and color is deeper.Plant weights can increase 30-40%, polyphenol content 25-35%, and disease resistance is remarkably reinforced.
General principle, principal character and the advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the simply explanation described in above-described embodiment and specification is originally
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these
Changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims
And its equivalent circle.
Claims (7)
1. a kind of streptomycete, purified and cultivated through primary dcreening operation, secondary screening, natural medium culture and actinomyces by production bacterial strain and auxiliary material
Obtained after process, it is characterised in that：Production bacterial strain is streptomycete Streptomyces sp.B-3-S, preservation on June 26 in 2017
In China typical culture collection center, preserving number：CCTCC No.M2017378.
A kind of 2. streptomycete as claimed in claim 1, it is characterised in that streptomycete (Streptomyces sp.) B-3-
S biological characteristics are：There is flourishing gas silk and base silk after separation on Gause I culture medium；Gas silk canescence, base silk are light yellow.
A kind of 3. streptomycete as claimed in claim 1, it is characterised in that streptomycete (Streptomyces sp.) B-3-
S-shaped state is characterized as：5-7 days diameters of streptomycete (Streptomyces sp.) B-3-S bacterium colonies can reach 3-4mm, and mycelia is just white
Color, it is rear light grey, villiform, reverse side yellowish often occur；Observation is with bacterium in flourishing hyperbranched base under the microscope
Silk and aerial hyphae, mycelia is not broken without tabula, and spore chain prosperity is in the shape of a spiral, verticillate；Spore circle or oval.
4. streptomycete (Streptomyces sp.) B-3-S described in claim 1 is improving disease resistance of plant with promoting plant
Application in growth.
5. application as claimed in claim 4, it is characterised in that：The plant is tea tree.
6. application as claimed in claim 5, it is characterised in that：The tea tree is Elevation.
7. streptomycete (Streptomyces sp.) B-3-S described in claim 1 is applied to the production of crops bio-bacterial manure
In technique.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710719891.XA CN107603904B (en) | 2017-08-21 | 2017-08-21 | Streptomyces and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710719891.XA CN107603904B (en) | 2017-08-21 | 2017-08-21 | Streptomyces and application thereof |
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| Publication Number | Publication Date |
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| CN107603904A true CN107603904A (en) | 2018-01-19 |
| CN107603904B CN107603904B (en) | 2021-02-09 |
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| CN201710719891.XA Active CN107603904B (en) | 2017-08-21 | 2017-08-21 | Streptomyces and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110468083A (en) * | 2019-06-28 | 2019-11-19 | 大连理工大学 | One plant of streptomycete DL70 separated and its biological and ecological methods to prevent plant disease, pests, and erosion growth-promoting application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104726362A (en) * | 2014-12-29 | 2015-06-24 | 中国热带农业科学院椰子研究所 | Streptomyces murinus and application thereof |
-
2017
- 2017-08-21 CN CN201710719891.XA patent/CN107603904B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104726362A (en) * | 2014-12-29 | 2015-06-24 | 中国热带农业科学院椰子研究所 | Streptomyces murinus and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| ANG LYU等: "Reveromycins A and B from Streptomyces sp. 3–10: Antifungal Activity against Plant Pathogenic Fungi In vitro and in a Strawberry Food Model System", 《FRONTIERS IN MICROBIOLOGY》 * |
| 李应祥等: "茶云纹叶枯病拮抗放线菌的筛选", 《安徽农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110468083A (en) * | 2019-06-28 | 2019-11-19 | 大连理工大学 | One plant of streptomycete DL70 separated and its biological and ecological methods to prevent plant disease, pests, and erosion growth-promoting application |
| CN110468083B (en) * | 2019-06-28 | 2022-04-01 | 大连理工大学 | Separated streptomycete DL70 and biocontrol growth promotion application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107603904B (en) | 2021-02-09 |
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