CN107596725A - The extracting method of free amino acid in a kind of fresh olive pomace - Google Patents
The extracting method of free amino acid in a kind of fresh olive pomace Download PDFInfo
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- CN107596725A CN107596725A CN201710789306.3A CN201710789306A CN107596725A CN 107596725 A CN107596725 A CN 107596725A CN 201710789306 A CN201710789306 A CN 201710789306A CN 107596725 A CN107596725 A CN 107596725A
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- amino acid
- free amino
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- fresh olive
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Abstract
The invention discloses a kind of extracting method of free amino acid in fresh olive pomace, it is used as Extraction solvent using the aqueous solution containing sodium isoascorbate, by the way of micro-wave digestion Free Amino Acids are extracted from fresh olive pomace, the purity of amino acid in obtained product is extracted up to more than 95%, species is up to 17 kinds, the amount of the amino acid in fresh fruit slag is greatly remained, total recovery rate is up to more than 35%.Processing is dried to fresh olive pomace with vacuum drying method in processing procedure; it is lower to the loss amount of amino acid than traditional heated-air drying; and the sodium isoascorbate in Extraction solvent is also further formed to aminoacid ingredient and protected; prevent oxidation of being failed during hot-working or ingress of air; finally realize the extraction of free amino acid in olive pomace in a manner of efficient, environmentally friendly, be the high-qualityization application extension direction of pomace.
Description
Technical field
The present invention relates to biological technical field, and in particular to the extraction side of free amino acid in a kind of fresh olive pomace
Method.
Background technology
According to statistics, 35-40kg pomaces will be produced after being extracted oil per 100kg fresh olives fruit, these pomace depolarization small parts
Outside as fertilizer or feed, the overwhelming majority is directly discarded, and this not only seriously pollutes environment, also result in the waste of resource,
Therefore, the effective utilization technology for developing olive pomace is needed badly.The deep development on olive pomace is using mainly at present
Concentrate on the extraction of the bioactive ingredients such as extraction and flavones, the polyphenol of Marc oil, but the big portion in the expressing process of early stage
The bioactive substances such as the grease and flavones, polyphenol that divide enter in olive oil, and polyphenol, Flavonoid substances easily aoxidize again, this
A little actual contents in pomace of active component are very low.According to《The main chemical compositions analysis of fresh olive pomace》One text is ground
Study carefully result to show in olive pomace in addition to fatty and a small amount of active component, the content of total amino acid up to 2.1%, including
17 kinds of amino acid, secondly content highest altogether containing 7 kinds of essential amino acids, wherein leucine is valine and isoleucine,
The content of threonine, lysine and methionine is relatively low, and leucine, isoleucine and valine are branched-chain amino acid, has and promotes
Muscle protein synthesis, reduce Adipogenesis, hypoglycemic, fatigue-resisting function.In nonessential amino acid, glutamic acid and lucid asparagus
The content of propylhomoserin is higher, and cystine, arginine, the content of tyrosine are relatively low, and the ratio of itself and optimum protein pattern approaches, and is tool
There is the protein of higher nutritive value.As can be seen here, olive pomace is a kind of accessory substance with better nutritivity value, is worth
Deep development is obtained to utilize.
Conventional to the extracting method of free amino acid in plant fresh sample at present has ultrasonic extraction, heating and refluxing extraction, leaching
The methods of formulation.Extraction solvent is generally water, alcohol, ether etc., and extraction conditions have conclusive influence to the extracted amount of amino acid, by
Aminoacid ingredient is different in not kindred plant, and corresponding extraction conditions are also not quite similar, only the composition point from plant in itself
Hand is separated out, suitable dicyandiamide solution and extraction conditions is established, the problems such as current recovery rate is low, extraction time is long could be improved, and
Being related to amino acid extraction in olive pomace, there is not been reported.
The content of the invention
The present invention is intended to provide a kind of method of the high efficiency extraction free amino acid from olive pomace, realizes olea europaea fruit
The high-quality utilization of slag.
The present invention is achieved by the following technical solutions：
The extracting method of free amino acid in a kind of fresh olive pomace, including it is drying and crushing, Microwave Extraction, Purification by filtration, dense
Contracting drying, specifically includes following steps：
（1）Drying and crushing：Drying process after fresh olive pomace degreasing is dried to pomace water content ＜ 13%, dried fruit
Ground-slag is broken, crosses 50-80 mesh sieves；
（2）Microwave Extraction：By step（1）In the pomace powder input micro-wave diminishing pot of preparation, according to 1：5-10 solid-liquid ratio adds
Extraction solvent, sealed after addition, be placed in microwave dissolver and handle, reprocessed 2-3 times, merging filtrate；
（3）Purification by filtration：Gained filtrate is purified with cation exchange resin column or activated carbon；
（4）It is concentrated and dried：Extract solution traditional vacuum after purification is concentrated to dryness, produces free amino acid finished product.
Step（1）Described in drying process be to be dried in vacuo fresh olive pomace under the conditions of 70-80 DEG C.
Described step（2）In Extraction solvent be 1-5% arabo-ascorbic acid sodium water solution, adjusted with glacial acetic acid or hydrochloric acid
It is 2-6 to be assigned to pH value.
Described step（2）The program of middle microwave dissolver is arranged to：50-80W、80℃、30-50min.
Described step（4）The temperature of middle vacuum concentration is 70 DEG C.
The present invention is using the aqueous solution containing sodium isoascorbate as Extraction solvent, from fresh oily olive by the way of micro-wave digestion
Free Amino Acids are extracted in olive pomace, the purity of amino acid is up to more than 95% in the product for extracting to obtain, and species is up to 17
Kind, the amount of the amino acid in fresh fruit slag is greatly remained, total recovery rate is up to more than 35%.With true in processing procedure
Processing is dried to fresh olive pomace in the method that sky is dried, lower to the loss amount of amino acid than traditional heated-air drying,
And the sodium isoascorbate in Extraction solvent is also further formed to aminoacid ingredient and protected, prevent empty in hot-working or contact
Fail and aoxidize during gas, the extraction of free amino acid in olive pomace is finally realized in a manner of efficient, environmentally friendly, is fruit
The high-qualityization application extension of slag direction.
Embodiment
The extracting method of free amino acid in a kind of fresh olive pomace, including drying and crushing, Microwave Extraction, filtered pure
Change, be concentrated and dried, specifically include following steps：
（1）Drying and crushing：By vacuum drying treatment, drying to pomace water content under the conditions of 70 DEG C after fresh olive pomace degreasing
＜ 13%, dried pomace crush, and cross 50 mesh sieves；
（2）Microwave Extraction：By step（1）In the pomace powder input micro-wave diminishing pot of preparation, according to 1：5 solid-liquid ratio adds extraction
Solvent, sealed after addition, be placed in microwave dissolver and handle, reprocessed 2 times, merging filtrate；
（3）Purification by filtration：Gained filtrate is purified with cation exchange resin column or activated carbon；
（4）It is concentrated and dried：Extract solution traditional vacuum after purification is concentrated to dryness, produces free amino acid finished product.
Step（2）The middle Extraction solvent used be 1% arabo-ascorbic acid sodium water solution, with glacial acetic acid or hydrochloric acid allocate to
PH value is 2.
Step（2）The program of middle microwave dissolver is arranged to：50W、80℃、50min.
Step（4）The temperature of middle vacuum concentration is 70 DEG C.
Claims (5)
1. the extracting method of free amino acid in a kind of fresh olive pomace, including drying and crushing, Microwave Extraction, Purification by filtration,
It is concentrated and dried, specifically includes following steps：
（1）Drying and crushing：Drying process after fresh olive pomace degreasing is dried to pomace water content ＜ 13%, dried fruit
Ground-slag is broken, crosses 50-80 mesh sieves；
（2）Microwave Extraction：By step（1）In the pomace powder input micro-wave diminishing pot of preparation, according to 1：5-10 solid-liquid ratio adds
Extraction solvent, sealed after addition, be placed in microwave dissolver and handle, reprocessed 2-3 times, merging filtrate；
（3）Purification by filtration：Gained filtrate is purified with cation exchange resin column or activated carbon；
（4）It is concentrated and dried：Extract solution traditional vacuum after purification is concentrated to dryness, produces free amino acid finished product.
2. the extracting method of free amino acid in a kind of fresh olive pomace as claimed in claim 1, it is characterised in that step
（1）Described in drying process be to be dried in vacuo fresh olive pomace under the conditions of 70-80 DEG C.
3. the extracting method of free amino acid in a kind of fresh olive pomace as claimed in claim 1, it is characterised in that described
The step of（2）In Extraction solvent be 1-5% arabo-ascorbic acid sodium water solution, it is 2- to be allocated with glacial acetic acid or hydrochloric acid to pH value
6。
4. the extracting method of free amino acid in a kind of fresh olive pomace as claimed in claim 1, it is characterised in that described
The step of（2）The program of middle microwave dissolver is arranged to：50-80W、80℃、30-50min.
5. the extracting method of free amino acid in a kind of fresh olive pomace as claimed in claim 1, it is characterised in that described
The step of（4）The temperature of middle vacuum concentration is 70 DEG C.
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| CN201710789306.3A CN107596725A (en) | 2017-09-05 | 2017-09-05 | The extracting method of free amino acid in a kind of fresh olive pomace |
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| CN201710789306.3A CN107596725A (en) | 2017-09-05 | 2017-09-05 | The extracting method of free amino acid in a kind of fresh olive pomace |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111481962A (en) * | 2020-04-23 | 2020-08-04 | 兰州海关技术中心 | Method for extracting salmonella-inhibiting substance from olive pomace and application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104001006A (en) * | 2014-05-14 | 2014-08-27 | 广西壮族自治区药用植物园 | Method for extracting free amino acid from asparagus cochinchinensis |
| CN104689106A (en) * | 2015-02-11 | 2015-06-10 | 海门市润圣纺织品有限公司 | Method for extracting free amino acid |
| CN104740296A (en) * | 2015-02-28 | 2015-07-01 | 周可幸 | Method for extracting amino acid from radices polygonati officinalis by microwave |
-
2017
- 2017-09-05 CN CN201710789306.3A patent/CN107596725A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104001006A (en) * | 2014-05-14 | 2014-08-27 | 广西壮族自治区药用植物园 | Method for extracting free amino acid from asparagus cochinchinensis |
| CN104689106A (en) * | 2015-02-11 | 2015-06-10 | 海门市润圣纺织品有限公司 | Method for extracting free amino acid |
| CN104740296A (en) * | 2015-02-28 | 2015-07-01 | 周可幸 | Method for extracting amino acid from radices polygonati officinalis by microwave |
Non-Patent Citations (1)
| Title |
|---|
| 万继锋 等: "橄榄果实中糖和氨基酸组成与含量分析", 《福建农业学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111481962A (en) * | 2020-04-23 | 2020-08-04 | 兰州海关技术中心 | Method for extracting salmonella-inhibiting substance from olive pomace and application |
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Application publication date: 20180119 |
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