CN107586728B - Cordyceps sinensis culture solution and preparation method thereof - Google Patents
Cordyceps sinensis culture solution and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of fungus fermentation, and particularly relates to a cordyceps sinensis culture solution, which contains fermentation liquor after hypha separation, wherein the fermentation liquor after hypha separation is recycled in proportion to be used as a preparation raw material return basic liquefied material liquid of a new culture solution, in addition, slag materials can be squeezed in a dilution ratio to obtain a squeezed juice, and the squeezed juice is returned to the basic liquefied material liquid for subsequent batching operation. Therefore, the culture solution provided by the invention is prepared by efficiently recycling the fermentation liquor and the squeezed liquor and matching with ingredients, so that the use of raw materials is saved, the cost of the raw materials for fermentation is finally reduced, the total production cost is further reduced, and the added value of products is improved.
Description
Technical Field
The invention relates to the technical field of fungus fermentation, in particular to a cordyceps sinensis culture solution and a preparation method thereof.
Background
The cordyceps sinensis can be used as both medicine and food for a long time, is a high-grade tonic, has mild medicine property, has wider medicinal and edible values than other tonic products, can be eaten all the year round, and is suitable for old, young, sick, weak and weak people without side effect. In recent years, the demand for cordyceps sinensis is greatly increased at home and abroad, but due to the special ecological environment and strict parasitism of cordyceps sinensis, the yield is quite limited, and resources are in short supply. In order to relieve the contradiction between supply and demand of the market, the artificial fermentation products of the cordyceps sinensis become more and more important, and the method for culturing the cordyceps sinensis mycelia by utilizing liquid submerged fermentation is an effective way for solving the shortage of the cordyceps sinensis medicinal resources. The research shows that the cordyceps sinensis mycelium obtained by artificial fermentation culture is basically consistent with the chemical composition and pharmacological action of natural cordyceps sinensis through toxicological, pharmacological and phytochemical research, and can replace the natural cordyceps sinensis to produce cordyceps products so as to make up for the shortage of natural resources.
The fermentation liquid of Cordyceps is obtained by culturing hirsutella sinensis strain by liquid fermentation, and separating mycelium. In the current fermentation production: (1) the utilization degree of fermentation liquor after mycelium extraction is limited, most of fermentation liquor directly enters a sewage treatment system as waste, so that not only is resource waste caused, but also the pollution to the environment is serious; (2) slag is generated after the raw materials are liquefied, and the slag is directly treated as waste materials, so that resource waste is caused. Therefore, the waste materials (fermentation liquor and liquefied slag) in the industrial production in the prior art are recycled and used for preparing a new culture medium, so that the resource waste is reduced, and the raw materials for production are saved.
In the prior art, a culture medium used for fermentation in the production of cordyceps sinensis powder is a common culture medium, but a culture medium is prepared again by recycling fermentation liquor after mycelium separation and liquefying feed liquid, and a technology of using the fermentation liquor and the liquefying slag as a new culture medium for fermenting the cordyceps sinensis powder is not reported, so that the fermentation liquor after mycelium separation and the liquefying slag are recycled as fermentation raw materials according to a certain proportion, and the consumption of the raw materials is reduced.
Disclosure of Invention
The invention aims to solve the problems and overcome the defects of the prior art, and provides a cordyceps sinensis culture solution capable of effectively improving the utilization rate of raw materials for producing cordyceps sinensis powder.
The technical scheme adopted by the invention for solving the technical problem is as follows:
a cordyceps sinensis culture solution is characterized in that: the culture solution contains fermentation liquor after the cordyceps sinensis mycelia are separated, and the fermentation liquor is obtained through the following processes:
(1) preparing a culture solution: respectively crushing and sieving corn and silkworm chrysalis, and taking 40-80 meshes of corn flour and silkworm chrysalis powder for later use;
1-3 wt% of corn flour, 1-3 wt% of silkworm chrysalis powder and 0.2-0.4 wt% of proteolytic enzyme are added into a liquefaction tank, water is replenished, heating is stopped when the temperature is raised to 100-105 ℃, the temperature is naturally reduced for 4-8 hours, liquefied feed liquid is obtained, separation is carried out, and supernatant is collected;
adding glucose, peptone and yeast extract into the collected supernatant to enable the content of reducing sugar in the mixed solution to be 15-30 g/L and the content of amino nitrogen to be 0.5-0.8 g/L, and adjusting the pH to be 7-7.5 to obtain a culture solution A;
(2) and (3) sterilization: sterilizing the culture solution A;
(3) fermentation: inoculating hirsutella sinensis strains into the sterilized culture solution A for fermentation culture;
(4) separation: after fermentation, solid-liquid separation is carried out, and mycelium and fermentation liquor are respectively recovered.
Further, the pressure is controlled to be 0.11-0.15 MPa during sterilization in the step (2), the temperature is controlled to be 121-125 ℃, and sterilization is carried out for 30-60 min.
Further, the hirsutella sinensis strain is subjected to scale-up culture step by step during the fermentation in the step (3), and the fermentation is finished when the concentration of the cordyceps sinensis mycelia in the tank reaches 30-50%.
Further, the culture solution further contains a liquefaction feed solution, and the liquefaction feed solution comprises a liquefaction supernatant and a liquefaction squeezed solution.
Meanwhile, the invention provides a preparation method of the cordyceps sinensis culture solution, and the culture solution is obtained by the following steps:
(1) crushing raw materials: respectively crushing and sieving corn and silkworm chrysalis, and taking 40-80 meshes of corn flour and silkworm chrysalis powder for later use;
(2) liquefaction: 1-3 wt% of corn flour, 1-3 wt% of silkworm chrysalis powder and 0.2-0.4 wt% of proteolytic enzyme are added into a liquefaction tank, water is replenished, heating is stopped when the temperature is raised to 100-105 ℃, and the temperature is naturally reduced for 4-8 hours to obtain liquefied feed liquid;
(3) separation: extracting the supernatant of the liquefied feed liquid; squeezing the slag, and respectively recovering squeezed liquid and squeezed slag;
(4) preparation: mixing 20-50 wt% of cordyceps sinensis fermentation liquor, 40-60 wt% of supernatant and 10-20 w% of squeezing liquid, adding auxiliary materials to enable the content of reducing sugar in the mixed liquid to be 15-30 g/L and the content of amino nitrogen to be 0.5-0.8 g/L, and adjusting the pH value to be 7-7.5 to obtain culture solution B.
And (3) further, adding water in an amount which is 0.5-2 times that of the slag charge when the slag charge is squeezed in the step (3), mixing and squeezing.
Furthermore, the auxiliary materials in the step (4) are glucose, peptone and yeast extract, and the auxiliary materials can be directly purchased and used from the market.
Compared with the prior art, the cordyceps sinensis culture solution provided by the invention has the following effects:
(1) according to the invention, the slag generated after raw materials are liquefied is diluted and proportioned according to the index standard of a normal basic liquefied material liquid, and the basic liquefied material liquid is returned to be used as a preparation raw material of a new culture solution, so that the liquefied material is saved;
(2) the fermentation liquor after hypha separation is recovered in proportion and returns to the liquefied feed liquid, and the recovery proportion and the post-ingredient proportion are determined to be used as the preparation raw materials of the culture solution for the next fermentation production, so that the raw material consumption and the waste material discharge are reduced;
(3) when the culture solution provided by the invention is used for producing the cordyceps sinensis powder, liquefied raw materials (corn and silkworm chrysalis) before optimization can be saved by about 50% after optimization, and the total raw materials in the total preparation are saved by about 20-25% on average.
Drawings
FIG. 1 is a process flow chart of the present invention for preparing fermentation broth of Cordyceps sinensis.
FIG. 2 is a flow chart of the preparation process of the cordyceps sinensis culture solution.
Detailed Description
In order to make the technical solutions of the present invention better understood by those skilled in the art, the present invention is further described below with reference to the accompanying drawings and examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The experimental methods in the following examples, which are not specified under specific conditions, are generally performed under conventional conditions.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. In addition, any methods and materials similar or equivalent to those described herein can be used in the practice of the present invention, and the preferred methods and materials are described herein for illustrative purposes only.
Example 1
The present embodiment provides a cordyceps sinensis culture solution, which contains a fermentation broth after mycelium separation, wherein the fermentation broth is obtained through the following processes:
(1) preparing a culture solution: respectively pulverizing corn and silkworm pupa, and sieving to obtain 40 mesh corn powder and silkworm pupa powder;
1 wt% of corn flour, 3 wt% of silkworm chrysalis meal, 0.2 wt% of proteolytic enzyme and the balance of water are added into a liquefaction tank, heating is stopped when the temperature is raised to 100 ℃, the temperature is naturally reduced for 4 hours to obtain liquefied feed liquid, separation is carried out, and supernatant liquid is collected;
adding glucose, peptone and yeast extract into the supernatant to be prepared, so that the content of reducing sugar in the mixed solution is 15-30 g/L, the content of amino nitrogen is 0.5-0.8 g/L, and adjusting the pH to 7-7.5 to obtain a culture solution A;
(2) and (3) sterilization: sterilizing the culture solution A at 121 deg.C under 0.11MPa for 30 min;
(3) fermentation: inoculating hirsutella sinensis strain into sterilized culture solution A, performing step-by-step enlarged culture, and stopping fermentation when the concentration of Cordyceps sinensis mycelia in the tank reaches 30%;
(4) separation: and after the fermentation is finished, carrying out solid-liquid separation, respectively recovering the mycelium and the fermentation liquor, and recycling the fermentation liquor.
Meanwhile, the culture solution also contains a liquefied feed liquid, and the liquefied feed liquid comprises a liquefied supernatant and a liquefied residue liquid, so that the invention provides a preparation method of the cordyceps sinensis culture solution, and the culture solution is obtained by the following steps:
(1) crushing raw materials: respectively pulverizing corn and silkworm pupa, and sieving to obtain 40 mesh corn powder and silkworm pupa powder;
(2) liquefaction: 1 wt% of corn flour, 3 wt% of silkworm chrysalis meal, 0.2 wt% of proteolytic enzyme and the balance of water are added into a liquefaction tank, heating is stopped when the temperature is raised to 100 ℃, and the temperature is naturally reduced for 4 hours to obtain liquefied feed liquid.
(3) Separation: collecting the supernatant from the liquefied material liquid; squeezing the slag, adding water with the weight 0.5 times of the weight of the slag during squeezing, mixing and squeezing, and respectively recovering squeezed liquid and squeezed slag;
(4) preparation: mixing 20 wt% of cordyceps sinensis fermentation liquor, 60 wt% of supernatant and 20 w% of squeezing liquid, adding glucose, peptone and yeast extract to ensure that the content of reducing sugar in the mixed liquor is 30g/L and the content of amino nitrogen is 0.8g/L, and adjusting the pH value to 7.5 to obtain culture solution B.
Example 2
The present embodiment provides a cordyceps sinensis culture solution, which contains a fermentation broth after mycelium separation, wherein the fermentation broth is obtained through the following processes:
(1) preparing a culture solution: respectively pulverizing corn and silkworm pupa, and sieving to obtain 70 mesh corn powder and silkworm pupa powder;
putting 2 wt% of corn flour, 2 wt% of silkworm chrysalis powder, 0.3 wt% of proteolytic enzyme and the balance of water into a liquefaction tank, heating to 103 ℃, stopping heating, naturally cooling for 6 hours to obtain liquefied feed liquid, separating, and collecting supernatant;
adding glucose, peptone and yeast extract into the supernatant to be prepared, so that the content of reducing sugar in the mixed solution is 25g/L and the content of amino nitrogen is 0.6g/L, and adjusting the pH value to 7.5 to obtain a culture solution A;
(2) and (3) sterilization: sterilizing the culture solution A at 0.13MPa and 123 deg.C for 45 min;
(3) fermentation: inoculating hirsutella sinensis strain into sterilized culture solution A, performing step-by-step enlarged culture, and stopping fermentation when the concentration of Cordyceps sinensis mycelia in the tank reaches 40%;
(4) separation: and after the fermentation is finished, carrying out solid-liquid separation, respectively recovering the mycelium and the fermentation liquor, and recycling the fermentation liquor.
Meanwhile, the culture solution provided by the invention also contains a liquefied feed solution, and the liquefied feed solution comprises a liquefied supernatant and a liquefied residue liquid, so that the embodiment provides a preparation method of the cordyceps sinensis culture solution, and the culture solution is obtained by the following steps:
(1) crushing raw materials: respectively pulverizing corn and silkworm pupa, and sieving to obtain 70 mesh corn powder and silkworm pupa powder;
(2) liquefaction: 2 wt% of corn flour, 2 wt% of silkworm chrysalis meal, 0.3 wt% of proteolytic enzyme and the balance of water are added into a liquefaction tank, heating is stopped when the temperature is raised to 103 ℃, and the temperature is naturally reduced for 6 hours to obtain liquefied feed liquid.
(3) Separation: collecting the supernatant from the liquefied material liquid; squeezing the slag, adding water with the same amount as the slag, mixing and squeezing, and respectively recovering squeezed liquid and squeezed slag;
(4) preparation: mixing 35 wt% of cordyceps sinensis fermentation liquor, 45 wt% of supernatant and 15 w% of squeezing liquid, adding glucose, peptone and yeast extract to ensure that the content of reducing sugar in the mixed liquor is 25g/L and the content of amino nitrogen is 0.6g/L, and adjusting the pH value to 7.5 to obtain culture solution B.
Example 3
The present embodiment provides a cordyceps sinensis culture solution, which contains a fermentation broth after mycelium separation, wherein the fermentation broth is obtained through the following processes:
(1) preparing a culture solution: respectively pulverizing corn and silkworm pupa, and sieving to obtain 70 mesh corn powder and silkworm pupa powder;
putting 2 wt% of corn flour, 2 wt% of silkworm chrysalis powder, 0.3 wt% of proteolytic enzyme and the balance of water into a liquefaction tank, heating to 103 ℃, stopping heating, naturally cooling for 6 hours to obtain liquefied feed liquid, separating, and collecting supernatant;
adding glucose, peptone and yeast extract into the supernatant to be prepared, so that the content of reducing sugar in the mixed solution is 25g/L and the content of amino nitrogen is 0.6g/L, and adjusting the pH value to 7 to obtain a culture solution A;
(2) and (3) sterilization: sterilizing the culture solution A at 125 deg.C under 0.15MPa for 60 min;
(3) fermentation: inoculating hirsutella sinensis strain into sterilized culture solution A, performing step-by-step amplification culture, and stopping fermentation when the concentration of Cordyceps sinensis mycelia in the tank reaches 50%;
(4) separation: and after the fermentation is finished, carrying out solid-liquid separation, respectively recovering the mycelium and the fermentation liquor, and recycling the fermentation liquor.
Meanwhile, the culture solution also contains a liquefied feed liquid, and the liquefied feed liquid comprises a liquefied supernatant and a liquefied residue liquid, so that the embodiment provides a preparation method of the cordyceps sinensis culture solution, and the culture solution is obtained by the following steps:
(1) crushing raw materials: respectively pulverizing corn and silkworm pupa, and sieving to obtain 80-mesh corn powder and silkworm pupa powder;
(2) liquefaction: and 3 wt% of corn flour, 1 wt% of silkworm chrysalis meal, 0.4 wt% of proteolytic enzyme and the balance of water are added into a liquefaction tank, heating is stopped when the temperature is raised to 105 ℃, and the temperature is naturally reduced for 8 hours to obtain liquefied feed liquid.
(3) Separation: collecting the supernatant from the liquefied material liquid; squeezing the slag, adding water 2 times of the weight of the slag during squeezing, mixing and squeezing, and respectively recovering squeezed liquid and squeezed slag;
(4) preparation: mixing 50 wt% of cordyceps sinensis fermentation liquor, 40 wt% of supernatant and 10 w% of squeezing liquid, adding glucose, peptone and yeast extract to enable the content of reducing sugar in the mixed liquor to be 15g/L and the content of amino nitrogen to be 0.5g/L, and adjusting the pH value to 7 to obtain culture solution B.
Example 4: liquid slag dilution proportioning test
Selecting materials sieved by a 70-mesh sieve, treating the materials with 0.3% of proteolytic enzyme K, collecting 10 batches of experimental data of the culture medium liquefaction materials, detecting the dry weight, the amino nitrogen and the reducing sugar of the soup bases, diluting the materials with tap water in a ratio of 1:1, detecting the same data after dilution, and continuously measuring the dry weight, the amino nitrogen and the reducing sugar content of ten batches of the liquefaction soup bases, wherein the data are as follows:
TABLE 1 detection data of dissolution rate of nutrients in liquefied slag and 1-fold dilution
TABLE 2 Shake flask yield comparison experiment (three average values) of slag diluted liquefied material and original clear liquid liquefied material
The experiment compares that the yield of the cordyceps sinensis hyphae cultured by two liquefied materials has no obvious difference with the current production, and the main physicochemical indexes of the fungus powder obtained after the culture are qualified according to the detection of pharmacopoeia standards.
The result shows that most of the liquefied slag materials are completely liquefied liquid materials and can be completely recycled through technical modification or improvement of separation technology. The concentration of the liquefied material after recovery is about 1 time higher than that of the original clear liquid liquefied material, and 1:1, and the culture test after dilution proves that the material has the same culture effect with the original clear liquid liquefied material and has no adverse effect on the quality and the yield of the product.
Example 5: returning and reusing fermented liquid with mycelium separated after fermentation
Considering that nutrient substances which are not completely utilized exist in fermentation liquor from which hyphae are separated after fermentation, the nutrient substances are used as culture raw materials for secondary fermentation, carbon sources and nitrogen sources with proper proportion are added, strains are inoculated after high-temperature sterilization, and the growth condition of the hyphae and the yield of the hyphae under test conditions are measured and used as test basis for the reutilization of the fermentation liquor in the future fermentation production.
And taking the residual quantity of the carbon and nitrogen sources in the normally extracted culture solution as reference, adding different quantities of carbon sources and nitrogen sources into subsequent ingredients, designing a three-factor three-level orthogonal test, and investigating the ingredient quantity of the subsequent materials at the return part of the fermentation solution.
Table 3 test design table for returned material proportioning ratio
The test uses a 500mL triangular flask, the loading amount is 200mL, the inoculation amount is 10%, and the dry weight, the reducing sugar content and the amino nitrogen content of each group of culture solution before inoculation are detected. Each set of orthogonal experiments was repeated in duplicate and placed on a sterile medium for small shake cultivation. And (5) placing the culture flask when the culture is carried out for 10-12 days, and detecting the dry weight of hyphae in each triangular flask and the content of amino nitrogen and reducing sugar in the culture solution.
TABLE 4 detection data of each experimental group before and after inoculation culture
TABLE 5 analysis of test results after inoculation and culture of fermentation broth returning material added with carbon source and nitrogen source in different proportions
Note: a is glucose, B is peptone, C is yeast extract; t is the mean of the sum of the factor test results;
r is the maximum to minimum number in the t value
From the analysis data in the above table, it can be seen that, taking the dry weight of the mycelia as a determination reference, the influence of the addition amount of glucose on the dry weight is the largest, and the dry weight of the mycelia is obviously increased along with the increase of the addition amount of glucose, which indicates that glucose is a main factor influencing the increase of the dry weight of the mycelia, and the addition amount of glucose becomes the most main constraint factor of the increase of the dry weight of the mycelia, so the addition amount of glucose needs to be increased; the addition of peptone also has a large influence on the dry weight of the mycelia, and the influence of yeast extract is relatively small.
Control tests, protocols and results for different glucose additions and normal manufacturing process recipes are shown in table 6:
TABLE 6 scheme and results for normal subsequent dosage and different proportions of return charge
And (4) experimental conclusion: the dry weight of the hyphae is greatly different from that of the hyphae in a control experimental group in actual production, so that the fermentation liquor at the fermentation end point is completely used as new culture, the feasibility is realized, the color of the hyphae is darker, the influence on the color of a finished product is larger, and the feasibility in actual production is not strong.
In addition, as can be seen from the dry weight data in the table, the addition amount of glucose has the greatest influence on the dry weight of hyphae, and the dry weight of hyphae is obviously increased along with the increase of the addition amount of glucose, which indicates that glucose is a main factor influencing the increase of the dry weight of hyphae, and the addition amount of glucose needs to be increased.
Example 6: fermentation liquor return ratio test
Considering that the recycled fermentation liquor is directly used for mycelium culture, the yield of mycelium is less than that of a normal control at the fermentation end point, the color of the mycelium is darker, the fermentation liquor is added into the liquefied material according to different proportions at this time, other nutrient components are added according to proportions for culture, and the addition amount of glucose is consistent with that in the current production.
Different test groups with the addition of 20%, 30%, 50% and 100% of the recovered fermentation liquor are designed to carry out comparison tests by taking a normal fermentation production process formula as a reference, and the formula of each test group is shown in the following table:
TABLE 7 experimental arrangement for fermentation liquor return ratio
Except that the adopted liquefied material is fresh liquefied material or the material is returned by fermentation liquor, other ingredients are carried out according to the original process. The packaging amount of each bottle is 220ml, the inoculation amount is 20ml, and the dry weight, the reducing sugar content and the amino nitrogen content of each group of culture solution before inoculation are detected. Two replicates of each test group were placed in a sterile room for small shake culture. When the culture is carried out for 10 days and 11 days, one parallel sample in each test group is taken respectively, and the dry weight of the hyphae in each triangular flask and the amino nitrogen and reducing sugar content in the culture solution are detected.
TABLE 8 detection data of each experimental group before and after inoculation of different fermentation broth return ratios
The results showed that the addition of a certain amount of fermentation broth to the liquefied material, the dry weight of the mycelia was comparable to that of the control group, demonstrating that partial return of the fermentation broth to the culture broth was feasible.
Example 7: determination test of raw material saving rate
And (3) comparison test: take the example of preparing 1 ton of fermentation culture solution of Cordyceps sinensis
TABLE 9 raw material saving comparative test
Taking the production of 1 ton of culture medium as an example: the preparation process of the complete culture solution is to liquefy and hydrolyze part of raw materials, and compound the obtained clear solution with the rest raw materials.
The composition of 1 ton of liquefied material is as follows, in the process of preparing liquefied material liquid, feeding 50% of planned feeding amount to produce 0.5 ton of fresh liquefied material, adding 20% (0.2 ton) of squeezed centrifugally diluted squeezed liquid and 30% (0.3 ton) of fermentation liquid reclaimed material to form new liquefied material clear liquid, and the subsequent compound raw materials are not changed, which is equivalent to saving about 50% of raw material input in the process of producing liquefied material liquid; other compound raw materials are added in the preparation, so that the raw materials of the liquefied material part are reduced by about 23.9 percent in the total production cost of the fermentation liquor.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention.
Claims (1)
1. A preparation method of a cordyceps sinensis culture solution is characterized by comprising the following steps: the culture solution contains fermentation liquor after cordyceps sinensis hypha separation, and the culture solution comprises: liquefying feed liquid, wherein the liquefying feed liquid comprises liquefied supernatant and liquefied extraction liquid, and the liquefied extraction liquid is obtained after the supernatant of the liquefying feed liquid is extracted; adding 0.5-2 times of water into the slag charge, mixing and squeezing to obtain the finished product;
the fermentation liquor is obtained by the following steps: (1) preparing a culture solution: respectively crushing and sieving corn and silkworm chrysalis, and taking 40-80 meshes of corn flour and silkworm chrysalis powder for later use; 1-3 wt% of corn flour, 1-3 wt% of silkworm chrysalis powder and 0.2-0.4 wt% of proteolytic enzyme are added into a liquefaction tank, water is replenished, heating is stopped when the temperature is raised to 100-105 ℃, the temperature is naturally reduced for 4-8 hours, liquefied feed liquid is obtained, separation is carried out, and supernatant is collected; adding glucose, peptone and yeast extract into the supernatant to enable the content of reducing sugar in the mixed solution to be 15-30 g/L and the content of amino nitrogen to be 0.5-0.8 g/L, and adjusting the pH to be 7-7.5 to obtain a culture solution A; (2) and (3) sterilization: sterilizing the culture solution A; sterilizing for 30-60 min at the pressure of 0.11-0.15 MPa and the temperature of 121-125 ℃; (3) fermentation: inoculating hirsutella sinensis strains into the sterilized culture solution A for fermentation culture; the hirsutella sinensis strain is subjected to scale-up culture step by step during fermentation, and the fermentation is finished when the concentration of the cordyceps sinensis mycelia reaches 30-50%; (4) separation: after fermentation, carrying out solid-liquid separation, and respectively recovering mycelium and fermentation liquor;
the liquefied feed liquid is obtained by the following steps: (1) crushing raw materials: respectively crushing and sieving corn and silkworm chrysalis, and taking 40-80 meshes of corn flour and silkworm chrysalis powder for later use; (2) liquefaction: 1-3 wt% of corn flour, 1-3 wt% of silkworm chrysalis powder and 0.2-0.4 wt% of proteolytic enzyme are added into a liquefaction tank, water is replenished, heating is stopped when the temperature is raised to 100-105 ℃, and the temperature is naturally reduced for 4-8 hours to obtain liquefied feed liquid; (3) separation: extracting the supernatant; squeezing the slag, and respectively recovering squeezed liquid and squeezed slag; (4) preparation: mixing 20-50 wt% of cordyceps sinensis fermentation liquor, 40-60 wt% of supernatant and 10-20 w% of squeezing liquid, adding auxiliary materials to enable the content of reducing sugar in the mixed liquid to be 15-30 g/L and the content of amino nitrogen to be 0.5-0.8 g/L, and adjusting the pH value to be 7-7.5 to obtain the liquefied feed liquid.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102220249A (en) * | 2011-06-13 | 2011-10-19 | 杭州柯氏生物科技有限公司 | Method for producing Hirsutella sinensis |
| CN103444434A (en) * | 2013-08-27 | 2013-12-18 | 王辉 | Fermentation raw material treatment method for improving yield and quality of cordyceps sinensis |
| CN105624200A (en) * | 2016-02-05 | 2016-06-01 | 浙江工业大学 | Recycling method of hirsutella sinensis fermentation filtrate |
| CN106190866A (en) * | 2016-08-11 | 2016-12-07 | 青海珠峰冬虫夏草工程技术研究有限公司 | A kind of reuse method of Cordyceps fermented waste fluid |
-
2017
- 2017-10-26 CN CN201711016779.6A patent/CN107586728B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102220249A (en) * | 2011-06-13 | 2011-10-19 | 杭州柯氏生物科技有限公司 | Method for producing Hirsutella sinensis |
| CN103444434A (en) * | 2013-08-27 | 2013-12-18 | 王辉 | Fermentation raw material treatment method for improving yield and quality of cordyceps sinensis |
| CN105624200A (en) * | 2016-02-05 | 2016-06-01 | 浙江工业大学 | Recycling method of hirsutella sinensis fermentation filtrate |
| CN106190866A (en) * | 2016-08-11 | 2016-12-07 | 青海珠峰冬虫夏草工程技术研究有限公司 | A kind of reuse method of Cordyceps fermented waste fluid |
Non-Patent Citations (2)
| Title |
|---|
| Optimization of Liquid Fermentation Conditions and Protein Nutrition Evaluation of Mycelium from the Caterpillar Medicinal Mushroom, Cordyceps militaris (Ascomycetes);J. Gang等;《International Journal of Medicinal Mushrooms》;20160131;第18卷(第8期);第745-752页 * |
| 蛹虫草深层发酵产虫草素培养基的优化;张楠等;《北方园艺》;20170315;第5卷;第134-141页 * |
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| CN107586728A (en) | 2018-01-16 |
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