CN107576706A - A kind of nitrate concentration sensor based on biological fuel cell and electricity production bacterium - Google Patents
A kind of nitrate concentration sensor based on biological fuel cell and electricity production bacterium Download PDFInfo
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- CN107576706A CN107576706A CN201711006903.0A CN201711006903A CN107576706A CN 107576706 A CN107576706 A CN 107576706A CN 201711006903 A CN201711006903 A CN 201711006903A CN 107576706 A CN107576706 A CN 107576706A
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- fuel cell
- biological fuel
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- nitrate concentration
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Abstract
The present invention discloses a kind of nitrate concentration sensor based on biological fuel cell and electricity production bacterium, including intake chamber and for according to output current power come monitor water inlet in nitrate concentration biological fuel cell, the biological fuel cell includes cathode chamber and the anode chamber connected with intake chamber, separated between the anode chamber and cathode chamber by amberplex, negative electrode is provided with cathode chamber, positive electrode, and positive electrode area load electricity-producing microorganism are provided with anode chamber；When the water containing nitrate enters in the anode chamber of biological fuel cell via intake chamber, electronics caused by the nitrate ion consumable anode in water, as electron acceptor ammonification is reduced, weakens the output current of biological fuel cell.Therefore, the change of the concentration of the nitrate in water can be judged according to the power of biological fuel cell output current, so as to monitor the nitrate concentration in water outlet quickly, precisely in real time, reduces the risk of health and environment pollution.
Description
Technical field
It is more particularly to a kind of thin based on biological fuel cell and electricity production the present invention relates to a kind of nitrate concentration monitoring device
The nitrate concentration sensor of bacterium.
Background technology
Azotate pollution is extremely serious in China's water body, and too high nitrate concentration can cause a series of health in water body
With environmental risk.Therefore the monitoring device for developing nitrate in water body efficiently, economic has been the task of top priority.Existing monitoring side
Method is based primarily upon AAS and electrode method.AAS is a kind of monitoring method of standard, but equipment price be present
Height, the problems such as detection process is cumbersome.Electrode method monitoring accuracy is low, easily by the interference of temperature, ion etc..Therefore, it is badly in need of exploitation one
The monitoring device that kind is cheap, monitors in real time.
The content of the invention
Goal of the invention：For the problems such as precision is low, detection process is cumbersome existing for existing nitrate concentration monitoring device, sheet
Invention provides a kind of nitrate concentration sensor based on biological fuel cell and electricity production bacterium.
Technical scheme：A kind of nitrate concentration sensing based on biological fuel cell and electricity production bacterium of the present invention
Device, including intake chamber and the biological fuel cell for monitoring nitrate concentration in water inlet according to output current power, should
Biological fuel cell includes cathode chamber and the anode chamber connected with intake chamber, is handed between the anode chamber and cathode chamber by ion
Change film to separate, be provided with negative electrode in cathode chamber, positive electrode, and positive electrode area load electricity-producing microorganism are provided with anode chamber；
When the water containing nitrate enters in the anode chamber of biological fuel cell, electronics is by the nitrate ion in water caused by anode
Consumption, weakens the output current of biological fuel cell.
Wherein, the electricity-producing microorganism of positive electrode area load can be the combination of single microorganism or multiple-microorganism.
Preferably, electricity-producing microorganism is electricity production bacterium, such as Shewanella oneidensis.
The sterilised membrane filter for being used for removing microorganism in water inlet can be provided between above-mentioned intake chamber and anode chamber, prevents anode chamber
Polluted by microorganism.The aperture of sterilised membrane filter is more preferably less than equal to 0.2 μm.
Preferably, nutritive salt or other nutriments are added in anode chamber, so that microorganism grows；Further, it is cloudy
Cushioning liquid is added in pole room, for absorbing H caused by anode+And electronics.
Inventive principle：Nitrate concentration sensor in the present invention is on the basis of biological fuel cell, on anode
Electricity-producing microorganism is loaded, the change of nitrate content in water is judged by electric current power；Specifically, the water containing nitrate
Enter anode chamber via intake chamber, the nitrate ion in water is reduced ammonification as electron acceptor, consumes in this process
Electronics, causes the output current to weaken.Therefore, the change of the concentration of the nitrate in water can be judged according to the change of output current
Change.
Beneficial effect：Compared with prior art, remarkable advantage of the invention is, nitrate concentration sensor of the invention
Use based on biological fuel cell and electricity production bacterium, by the change of the current strength of biological fuel cell can quickly, it is real-time,
The accurate concentration for knowing nitrate in reaction water, moreover, device overall structure is simple, cost is low, it is a kind of excellent biology biography
Sensor.
Brief description of the drawings
Fig. 1 is that a kind of principle of nitrate concentration sensor based on biological fuel cell and electricity production bacterium of the present invention is shown
It is intended to；
Fig. 2 is the topology layout figure when nitrate concentration sensor of the present invention uses horizontal water distribution manner, arrow in figure
Direction represents water inlet direction；
The correlation curve of nitrate concentration and electric current in the water inlet that Fig. 3 is measured when being 25 DEG C of water temperature in embodiment 1；
The correlation curve of nitrate concentration and electric current in the water inlet that Fig. 4 is measured when being 15 DEG C of water temperature in embodiment 2；
The correlation curve of nitrate concentration and electric current in the water inlet that Fig. 5 is measured when being 5 DEG C of water temperature in embodiment 3.
Embodiment
Technical scheme is described further with specific embodiment below in conjunction with the accompanying drawings.
Such as Fig. 1, the nitrate concentration sensor main of the invention based on biological fuel cell and electricity production bacterium will be by three
It is grouped into：Cathode chamber 1, anode chamber 2 and intake chamber 3, are separated by amberplex 6 between cathode chamber 1 and anode chamber 2, entered
Tank 3 connects with anode chamber 2.
The structure of above-mentioned three big component, such as cathode chamber 1, anode chamber 2, the shape of intake chamber 3, arrangement are not limited to figure
Form in 1, or other arbitrary structures, such as Fig. 2, as long as the function of the present invention can be realized；At above-mentioned three big groups
On the basis of part, it can also increase some accessories, such as temperature control, nutriment addition equipment.
Negative electrode 4 and positive electrode 5 are set respectively in cathode chamber 1 and anode chamber 2, and the area load of positive electrode 5 produces electricity micro- life
Thing, connected between negative electrode 4 and positive electrode 5 by current detecting equipment, such as ammeter, form biological fuel cell.Positive electrode 5
The microorganism of area load is efficient electricity-producing microorganism, can be the combination of single microorganism or multiple-microorganism；It such as can be production
Electric bacterium Shewanella oneidensis.
Nutritive salt or other nutriments can be added in anode chamber 2, so that microorganism grows；It can be added in cathode chamber 1
Cushioning liquid, for absorbing H caused by anode+And electronics.
It can be connected between intake chamber 3 and anode chamber 2 by sterilised membrane filter 7, sterilised membrane filter 7 can remove micro- life in water inlet
Thing, prevent that anode chamber 2 from being polluted by microorganism.The aperture of sterilised membrane filter 7 is general≤and 0.2 μm.
Such as Fig. 1, intake chamber 3 can be by the way of lower water inlet and upper water-out, but is not limited to such a water distribution manner.Contain nitric acid
The water of salt enters anode chamber 2 via intake chamber 3, and the nitrate ion in water is reduced ammonification as electron acceptor, in this mistake
Electronics is consumed in journey, causes output current to weaken.Therefore, nitrate concentration sensor of the invention can be according to output current
Power judge nitrate in water concentration change, it is dense so as to quick, precisely in real time in monitoring water outlet nitrate
Degree, reduce the risk of health and environment pollution.
Embodiment 1
Exemplary device uses the topology layout of horizontal water distribution, such as Fig. 2, and anode chamber 2 adds nutritive salt, and cathode chamber 1 adds phosphorus
Acid buffer；The surface seeding of positive electrode 5 electricity production bacterium Shewanella oneidensis；Cathode connection stake and anode terminal
Between connected by ammeter.Water temperature of intaking is 25 DEG C, and nitrate concentration is 0~2mg/L, measure electric current and nitrate in water inlet
The correlation of concentration, as a result such as Fig. 3.
Embodiment 2
Exemplary device uses the topology layout of horizontal water distribution, such as Fig. 2, and anode chamber 2 adds nutritive salt, and cathode chamber 1 adds phosphorus
Acid buffer；The surface seeding of positive electrode 5 electricity production bacterium Shewanella oneidensis；Cathode connection stake and anode terminal
Between connected by ammeter.Water temperature of intaking is 15 DEG C, and nitrate concentration is 0~2mg/L, measure electric current and nitrate in water inlet
The correlation of concentration, as a result such as Fig. 4.
Embodiment 3
Exemplary device uses the topology layout of horizontal water distribution, such as Fig. 2, and anode chamber 2 adds nutritive salt, and cathode chamber 1 adds phosphorus
Acid buffer；The surface seeding of positive electrode 5 electricity production bacterium Shewanella oneidensis；Cathode connection stake and anode terminal
Between connected by ammeter.Water temperature of intaking is 5 DEG C, and nitrate concentration is 0~2mg/L, measure electric current and nitrate in water inlet
The correlation of concentration, as a result such as Fig. 5.
Claims (7)
1. a kind of nitrate concentration sensor based on biological fuel cell and electricity production bacterium, it is characterised in that including intake chamber
(3) and for according to output current power come monitor water inlet in nitrate concentration biological fuel cell, the bio-fuel electricity
Pond includes cathode chamber (1) and the anode chamber (2) connected with intake chamber (3), passes through between the anode chamber (2) and cathode chamber (1)
Amberplex (6) is separated, and negative electrode (4) is provided with the cathode chamber (1), positive electrode (5) is provided with anode chamber (2), and
Positive electrode (5) area load electricity-producing microorganism；When the anode chamber (2) that the water containing nitrate enters biological fuel cell is interior,
Electronics caused by anode is consumed by the nitrate ion in water, weakens the output current of biological fuel cell.
2. the nitrate concentration sensor according to claim 1 based on biological fuel cell and electricity production bacterium, its feature
It is, the electricity-producing microorganism of positive electrode (5) area load is the combination of single microorganism or multiple-microorganism.
3. the nitrate concentration sensor according to claim 2 based on biological fuel cell and electricity production bacterium, its feature
It is, the electricity-producing microorganism is electricity production bacterium Shewanella oneidensis.
4. the nitrate concentration sensor according to claim 1 based on biological fuel cell and electricity production bacterium, its feature
It is, the sterilised membrane filter (7) for being used for removing microorganism in water inlet is provided between the intake chamber (3) and anode chamber (2).
5. the nitrate concentration sensor according to claim 4 based on biological fuel cell and electricity production bacterium, its feature
It is, the aperture of the sterilised membrane filter (7) is less than or equal to 0.2 μm.
6. the nitrate concentration sensor according to claim 1 based on biological fuel cell and electricity production bacterium, its feature
It is, nutritive salt or other nutriments beneficial to microorganism growth is loaded with the anode chamber (2).
7. the nitrate concentration sensor according to claim 1 based on biological fuel cell and electricity production bacterium, its feature
It is, is loaded with the cathode chamber (1) for absorbing H caused by anode+With the cushioning liquid of electronics.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711006903.0A CN107576706A (en) | 2017-10-25 | 2017-10-25 | A kind of nitrate concentration sensor based on biological fuel cell and electricity production bacterium |
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| CN201711006903.0A CN107576706A (en) | 2017-10-25 | 2017-10-25 | A kind of nitrate concentration sensor based on biological fuel cell and electricity production bacterium |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104064794A (en) * | 2014-07-14 | 2014-09-24 | 中国海洋大学 | Microbial fuel cell capable of repairing nitrate polluted underground water in situ |
| CN104681844A (en) * | 2015-02-15 | 2015-06-03 | 中国海洋大学 | Microbial fuel cell for in-situ remediation of nitrate pollution of underground water |
| CN104773827A (en) * | 2015-04-13 | 2015-07-15 | 中国科学院过程工程研究所 | System and method for treating wastewater produced by production of bio-cathode type microbial fuel cells |
-
2017
- 2017-10-25 CN CN201711006903.0A patent/CN107576706A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104064794A (en) * | 2014-07-14 | 2014-09-24 | 中国海洋大学 | Microbial fuel cell capable of repairing nitrate polluted underground water in situ |
| CN104681844A (en) * | 2015-02-15 | 2015-06-03 | 中国海洋大学 | Microbial fuel cell for in-situ remediation of nitrate pollution of underground water |
| CN104773827A (en) * | 2015-04-13 | 2015-07-15 | 中国科学院过程工程研究所 | System and method for treating wastewater produced by production of bio-cathode type microbial fuel cells |
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Application publication date: 20180112 |