CN107561110B - A kind of discrimination method being used for chaste honey, rape honey and acacia honey based on nuclear magnetic resonance technique - Google Patents
A kind of discrimination method being used for chaste honey, rape honey and acacia honey based on nuclear magnetic resonance technique Download PDFInfo
- Publication number
- CN107561110B CN107561110B CN201710742918.7A CN201710742918A CN107561110B CN 107561110 B CN107561110 B CN 107561110B CN 201710742918 A CN201710742918 A CN 201710742918A CN 107561110 B CN107561110 B CN 107561110B
- Authority
- CN
- China
- Prior art keywords
- honey
- nuclear magnetic
- chaste
- rape
- acacia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of discrimination methods based on nuclear magnetic resonance technique for chaste honey, rape honey and acacia honey, and honey sample is configured to the solution of 0.03-0.5g/L with heavy water after filtering；The phosphate buffer solution that concentration is 0.5-2.0 mol/L is added, wherein, sodium azide containing 3- trimethylsilyl -1- propyl sulfonic acid sodium inner mark solution and 0.1-3.0 mM that volume fraction is 0.05-0.3% in phosphate buffer solution, mixing, adjustment pH is 2.5-4.0, measures to obtain nuclear magnetic resoance spectrum through nuclear magnetic resonance spectrometer；The chemical shift of honey sample marker is analyzed,1The deviation △ δ that H chemical shift δ is 7.0ppm~7.5ppm is chaste honey within 0.02-0.05ppm range, is otherwise rape honey or acacia honey.This method can analyze a large amount of samples in the short time, quickly identify point chaste honey and rape honey and acacia honey.
Description
Technical field
The present invention relates to being originally related to belonging to food inspection, be more particularly to it is a kind of based on nuclear magnetic resonance technique for the twigs of the chaste tree
The discrimination method of honey, rape honey and acacia honey.
Background technique
It is provided in honey standard GB/T 14963-2011GB, honey is nectar, the secretion of a kind of honeybee herborization
Or honeydew, after mixing with itself secretion, natural sweet substance made of sufficiently being made is a kind of natural taste full of nutrition
Support food.Hua Yuan is variant, i.e. the metabolism of plant is variant, thus the nectar compositions that honeybee is adopted will be different, and honey is usual
Classified according to nectariferous plant, rape honey, acacia honey, chaste honey, Mel Jujubae, eucalyptus honey etc. can be divided into.In recent years, domestic and
International market constantly expands the demand of honey, however the yield of honey is but difficult to meet the market demand.Due to China region
It is vast, with a varied topography, weather is various, honey honey kind and the place of production have diversified characteristic, and added value of product also generates difference.
Under the driving of tremendous economic interests, other low-quality honey are added in high-quality honey and are filled with secondary by many criminals
It is good, achieve the effect that mix the spurious with the genuine.The severe jamming market order of honey product and the export trade of China's honey product.Cause
This needs to develop effective, reliable method to identify the honey of different plant sources.
Identification for honey honey kind, most effective means are the spirits that discovery can significantly distinguish specific sweet kind of parameter
Quick, reliable chemical marker.Conventional method identifies mainly by sense organ and pollen analysis.Sense organ identification be by seeing, hearing, product,
It was found that variety classes honey existing some differences in terms of color, smell, mouthfeel, and then distinguish and identify.This method has very big
Limitation depends on the experience of testing staff individual；Pollen analysis is to observe pollen cell in honey under the microscope
Form can identify the honey kind of honey since the pollen of every kind of nectariferous plant has the feature of oneself.But the bee of some honey kind
Bee pollen is similar, and this method is difficult to distinguish.However, the international standard in relation to special marker is not promulgated also.European Council's hair
The determining honey honey kind mirror method for distinguishing of cloth, further includes measurement general flavone, volatile component, amino acid, metal and nonmetallic trace
Secondary element.However these methods lack sensitive reliable marker, the identification for honey honey kind, accuracy, sensitivity and
Reliability will all substantially reduce.
Nuclear magnetic resonance (NMR) is a kind of very important research and analysis testing tool, in chemistry, biology, medicine etc.
It is used widely in field, there are many advantages: being able to maintain the integrality of test object, do not destroy the property of sample；To sample
This requirement is not harsh, can quickly handle, and repeats to test, greatly reduces the consumption of experiment；The identification to substance can be completed at the same time
It is efficiently quick with the measurement of content of material；With fabulous resolution ratio.The present invention filters out chaste honey with nuclear magnetic resonance technique
Characteristic indication object, thus establish quickly distinguish chaste honey, rape honey and acacia honey method.
Summary of the invention
The present invention provides: a kind of discrimination method being used for chaste honey, rape honey and acacia honey based on nuclear magnetic resonance technique,
Technically reliable can analyze a large amount of samples, quickly identify point chaste honey and rape honey and acacia honey, avoid subjectivity in the short time
The influence of factor, human error, accuracy is high, conclusion science is reliable.
To solve the above-mentioned problems, the technical scheme adopted by the invention is that such, one kind being based on nuclear magnetic resonance technique
For the discrimination method of chaste honey, rape honey and acacia honey, include the following steps,
1) honey sample that will be stirred evenly, filter-cloth filtering are removed in honey after solid impurity, are configured to heavy water
The solution of 0.03-0.5g/L；The honey sample is chaste honey, rape honey and acacia honey；
2) the above-mentioned solution of certain volume is taken, the phosphate buffer solution that concentration is 0.5-2.0 mol/L is added, the two
Volume ratio is 800-1500:100-300, wherein the 3- trimethyl for being 0.05-0.3% containing volume fraction in phosphate buffer solution
The sodium azide of silylation -1- propyl sulfonic acid sodium inner mark solution and 0.1-3.0 mM, for vortex oscillation to uniform mixing, adjustment is final
The pH value of solution is 2.5-4.0, is placed in nuclear magnetic tube；
3) nuclear magnetic tube is placed in nuclear magnetic resonance spectrometer, detection obtains nuclear magnetic resoance spectrum；The analysis of NMR spectrum
Condition are as follows: popped one's head in using BBI；Measuring temperature: 20-35 DEG C of room temperature；Ambient humidity: 20-35%;400 MHz of observed frequency；
Water peak is suppressed using presaturation method；Pulse protocol are as follows: noesygppr1d or zg30 or zgpr；Spectrum width: 6410 Hz；
90 ° of pulse widths: the μ of pl=6. 45 s;Pulse delay time: the s of dl=15；Launching centre: O1=4.7；Accumulative frequency:
16 or 64；
4) chemical shift of honey sample marker is analyzed,1H chemical shift δ is 7.0ppm~7.5ppm ± 0.02-
Having multiplet within 0.05ppm range is chaste honey, is otherwise rape honey or acacia honey.
Above-mentioned honey sample is divided into nodeless mesh and crystallization, to the honey sample of nodeless mesh, stirs evenly before filtering
?；It to the honey sample for having crystallization, in closed situation, is placed in the water-bath no more than 40 DEG C -60 DEG C and warms, vibrate, to
Sample stirs evenly after all melting, and is rapidly cooled to room temperature；
Preferably, honey sample filtering uses aperture for the nylon filtering cloth of 0.10mm-0.14mm.
Preferably concentration used by the pH value of step 2 adjustment final solution is 1.0 mol/L hydrochloric acid or Strong oxdiative sodium.
In order to ensure the accuracy of looked for marker, the case where we have investigated the honey sample of different sources, honey sample
Product provenance is in Jiangsu, Henan, Xinjiang, Sichuan, Inner Mongol, Hubei, Liaoning, Jilin, Shaanxi or Shandong.
By comparing the nmr spectrum of the chaste honey of research different sources, find all chaste honeys in this chemical shift
All there is higher corresponding multiplet out, meanwhile, exist by comparing research different sources rape honey and acacia honey1H chemical shift
Whether δ has interference for 7.0ppm~7.5ppm's, the results showed that all rape honeys and acacia honey are all noiseless herein.Therefore
It is final to determine1The multiplet that H chemical shift δ is 7.0ppm~7.5ppm is the distinctive characteristic indication object of chaste honey, Ke Yizuo
For the feature difference substance for distinguishing chaste honey and rape honey and acacia honey.
The utility model has the advantages that the technology of the present invention is reliable, a large amount of samples can be analyzed in the short time, quickly identify point chaste honey and oil
Dish honey and acacia honey avoid the influence of subjective factor, human error, and accuracy is high, conclusion science is reliable.
Detailed description of the invention
Fig. 1 is the nuclear magnetic spectrogram of Henan chaste honey；
Fig. 2 is the nuclear magnetic spectrogram of Henan rape honey；
Fig. 3 is the nuclear magnetic spectrogram of Henan acacia honey.
Specific embodiment
By following specific embodiments to further explanation of the present invention, and advantage of the present invention is proved.
Experimental facilities includes: 400 type Nuclear Magnetic Resonance of Advance (Brooker,Switzerland company), 14.1 T superconducting magnets,
5 mm double-core z- gradient probes and 2.3 controlling test of Topspin and data processing software；5mm nuclear magnetic resonance sample pipe；At a high speed
Centrifuge (Sigma, German company)；Turbine mixer (XW-80A type, Instrument Factory, Shanghai Medical Science Univ.)；LP403 assay balance
(Sai Duolisi, German company)；
Test solvent includes: that deuterated water (deuterium band width is 99.8% also known as heavy water) is purchased from Cambrideg Isotope
Laboratories company；3- trimethylsilyl -1- propyl sulfonic acid sodium (TSPSA) is purchased from Aldich-Sigma company；Phosphoric acid
Hydrogen dipotassium and sodium dihydrogen phosphate (excellent pure grade) are purchased from Aldich-Sigma company.
1, the confirmation of chaste honey marker
The acquisition of 1.1 honey samples
Voluntarily acquisition honey sample total 60 include chaste honey 20, rape honey 20 and acacia honey 20；It is related to
The place of production includes: Jiangsu, Henan, Xinjiang, Sichuan, Inner Mongol, Hubei, Liaoning, Jilin, Shaanxi, Shandong；
1.2. the pre-treatment of honey sample
To nodeless mesh in honey sample, it is stirred for before filtering uniformly；And to there is crystallization, in closed situation
Under, it is placed in the water-bath no more than 60 DEG C and warms, vibrate, stirred evenly after sample all melts, be rapidly cooled to room temperature；Through above-mentioned
Treated, and known honey sample removes solid impurity in honey, takes 0.1 through the filtering of the aperture 0.10mm-0.14mm nylon filtering cloth
1 mL heavy water is added in centrifuge tube in g sample, and dissolution is complete；
Above-mentioned 800 μ l of solution is taken, the phosphate buffer solution (pH=2.0) that 100 μ l concentration are 0.5 mol/L is added, wherein
In buffer solution containing volume fraction be 0.05% 3- trimethylsilyl -1- propyl sulfonic acid sodium inner mark solution and 0.1 mM it is folded
Sodium nitride, vortex oscillation, then with the hydrochloric acid of 1.0 mol/L or Strong oxdiative sodium, adjust the pH value of final solution to uniform mixing
Be 2.5, take wherein 600 μ l in nuclear magnetic tube.
1.3. the foundation of nmr analysis
It adjusts magnetic resonance spectroscopy instrument and nuclear magnetic tube is placed in nuclear magnetic resonance chemical analyser, rotation nuclear magnetic tube is in case NMR is examined
It surveys, obtains the nuclear magnetic signal of honey sample；The analysis condition of NMR spectrum are as follows: popped one's head in using BBI；Measuring temperature (probe
Temperature): 20-35 DEG C of room temperature；Ambient humidity: 20-35%;400 MHz of observed frequency；Water peak is suppressed using presaturation method；
Pulse protocol are as follows: noesygppr1d or zg30 or zgpr；Spectrum width: 6410 Hz；90 ° of pulse widths: the μ of pl=6. 45
s;Pulse delay time: the s of dl=15；Launching centre: O1=4.7；Accumulative frequency: 16 or 64.
1.4. spectrogram pre-processes
Carrying out Fourier to resulting NMR signal with the data processing software that nuclear magnetic resonance spectrometer carries becomes to change,
Transformation points are 131072, and index linewidth factor is 1Hz, obtain the nuclear magnetic spectrum of sample.
With topspin software, (each parameter setting is as follows in Topspin software: PULPROG=Jresgpprqf, AQ_mod
=DQD, TD=32K, NS=8*N, DS=4, the μ of s, DE=4 of Hz, D1=3 of TD0=1, SW=6000 s, D8=0.1s, signal
It is 16 times cumulative)) the original fingerprint map of acquisition is pre-processed, including spectral peak alignment, baseline correction and phasing.With 3-
Trimethylsilyl -1- propyl sulfonic acid sodium does internal standard compound, and chemical shift is set as 0.00 carry out spectral peak alignment；Baseline correction selection
Polynomial fit fit approach；Mutually correction selection global and metabonomics algorithm elder generation Automatic Optimal, it is rear for spy
Determine region and carry out manual correction, makes integrated value positive value as much as possible.
1.5. the comparison of nmr spectrum
By comparing research chaste honey, the nmr spectrum of rape honey and acacia honey, discovery chaste honey exists1H chemical potential
Moving δ, there are the higher multiplets of response between 7.0ppm~7.5ppm.
By comparing the nmr spectrum of the chaste honey of research different sources, find all chaste honeys in this chemical shift
All there is higher corresponding multiplet out,
Meanwhile existing by comparing research different sources rape honey and acacia honey1H chemical shift δ be 7.0ppm~
Whether 7.5ppm's has interference, the results showed that all rape honeys and acacia honey are all noiseless herein.
Therefore, final to determine1The multiplet that H chemical shift δ is 7.0ppm~7.5ppm is the distinctive feature of chaste honey
Marker can be used as the feature difference substance for distinguishing chaste honey and rape honey and acacia honey.Such as the core that Fig. 1 is Henan chaste honey
Magnetic spectrum figure；Fig. 2 is the nuclear magnetic spectrogram of Henan rape honey；Fig. 3 is the nuclear magnetic spectrogram of Henan acacia honey.
2, the detection of actual sample
With foundation identify chaste honey, the method for quick identification of rape honey and acacia honey be used for unknown honey sample into
Row detection.20 parts of unknown honey samples are operated by the method for above-mentioned steps 1,2 ~ 1.4, obtain the nuclear-magnetism of all samples
Resonate spectrogram, and the marker according to the chaste honey found in step 5 judges unknown sample.Under identical experiment condition
The sample and chaste honey marker of detection1H chemical shift δ is the deviation △ δ of 7.0ppm~7.5ppm in 0.02-0.05ppm
Within range, then it is assumed that it is chaste honey, is otherwise rape honey or acacia honey, it can be quickly from rape honey and acacia honey according to this
In identify chaste honey, concrete outcome is as shown in table 1, and 10 chaste honeys have all obtained good identification in 20 samples, identify
Accuracy is 100%.
Table 1
| Sample type | Legitimate reading | Detect result |
| Chaste honey (10) | The twigs of the chaste tree | The twigs of the chaste tree |
| Rape honey and acacia honey (10) | Rape or acacia | Rape or acacia |
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (5)
1. a kind of discrimination method for being used for chaste honey, rape honey and acacia honey based on nuclear magnetic resonance technique, which is characterized in that including
Following steps,
1) honey sample that will be stirred evenly, filter-cloth filtering remove in honey after solid impurity, are configured to 0.03- with heavy water
The solution of 0.5g/L；The honey sample is chaste honey, rape honey and acacia honey；
2) the above-mentioned solution of certain volume is taken, the phosphate buffer solution that concentration is 0.5-2.0 mol/L, the volume of the two is added
Than for 800-1500:100-300, wherein the 3- trimethyl silicane for being 0.05-0.3% containing volume fraction in phosphate buffer solution
The sodium azide of alkyl -1- propyl sulfonic acid sodium inner mark solution and 0.1-3.0 mM, vortex oscillation to uniform mixing, adjustment are final molten
The pH value of liquid is 2.5-4.0, is placed in nuclear magnetic tube；
3) nuclear magnetic tube is placed in nuclear magnetic resonance spectrometer, detection obtains nuclear magnetic resoance spectrum；The analysis condition of NMR spectrum
Are as follows: it is popped one's head in using BBI；Measuring temperature: 20-35 DEG C of room temperature；Ambient humidity: 20-35%;400 MHz of observed frequency；Using
Presaturation method suppresses water peak；Pulse protocol are as follows: noesygppr1d or zg30 or zgpr；Spectrum width: 6410 Hz；90 ° of arteries and veins
Rush width: the μ of pl=6. 45 s;Pulse delay time: the s of dl=15；Launching centre: O1=4.7；Accumulative frequency: 16 or
64；
4) chemical shift of honey sample marker is analyzed,1H chemical shift δ is 7.0ppm~7.5ppm ± 0.02-0.05ppm model
Having multiplet within enclosing is chaste honey, is otherwise rape honey or acacia honey.
2. a kind of identification for being used for chaste honey, rape honey and acacia honey based on nuclear magnetic resonance technique according to claim 1
Method, which is characterized in that for the honey sample of crystallization, before stirring evenly, in closed situation, be placed in no more than 40-60 DEG C
Water-bath in warm, vibrate, to sample all melt, after mixing evenly, then be rapidly cooled to room temperature.
3. a kind of identification for being used for chaste honey, rape honey and acacia honey based on nuclear magnetic resonance technique according to claim 1
Method, which is characterized in that honey sample filtering uses aperture for the nylon filtering cloth of 0.10mm-0.14mm.
4. a kind of identification for being used for chaste honey, rape honey and acacia honey based on nuclear magnetic resonance technique according to claim 1
Method, which is characterized in that it is 1.0 mol/L hydrochloric acid or Strong oxdiative that step 2, which adjusts concentration used by the pH value of final solution,
Sodium.
5. a kind of identification for being used for chaste honey, rape honey and acacia honey based on nuclear magnetic resonance technique according to claim 1
Method, which is characterized in that honey sample provenance is in Jiangsu, Henan, Xinjiang, Sichuan, Inner Mongol, Hubei, Liaoning, Jilin, Shan
West or Shandong.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710742918.7A CN107561110B (en) | 2017-08-25 | 2017-08-25 | A kind of discrimination method being used for chaste honey, rape honey and acacia honey based on nuclear magnetic resonance technique |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710742918.7A CN107561110B (en) | 2017-08-25 | 2017-08-25 | A kind of discrimination method being used for chaste honey, rape honey and acacia honey based on nuclear magnetic resonance technique |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107561110A CN107561110A (en) | 2018-01-09 |
| CN107561110B true CN107561110B (en) | 2019-05-10 |
Family
ID=60976932
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710742918.7A Active CN107561110B (en) | 2017-08-25 | 2017-08-25 | A kind of discrimination method being used for chaste honey, rape honey and acacia honey based on nuclear magnetic resonance technique |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107561110B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108872289B (en) * | 2018-04-13 | 2021-03-02 | 南京海关动植物与食品检测中心 | Manuka honey identification method based on nuclear magnetic resonance technology |
| CN108614000A (en) * | 2018-04-13 | 2018-10-02 | 江苏出入境检验检疫局动植物与食品检测中心 | Manuka honey discrimination method based on SIMCA models |
| CN109444200B (en) * | 2018-12-21 | 2022-02-11 | 钛和中谱检测技术(江苏)有限公司 | Method for identifying northeast basswood honey by adopting nuclear magnetic resonance hydrogen spectrum fingerprint |
| RU2696772C1 (en) * | 2019-01-22 | 2019-08-06 | Федеральное государственное автономное образовательное учреждение высшего образования "Северо-Восточный федеральный университет имени М.К.Аммосова" | Method for determining the thermal effect on honey |
| CN111380895A (en) * | 2020-04-01 | 2020-07-07 | 江苏中谱检测有限公司 | Nuclear magnetic resonance hydrogen spectrometry for identifying whey protein powder added in formula powder |
| CN111380896A (en) * | 2020-04-07 | 2020-07-07 | 江苏中谱检测有限公司 | Method for identifying mature and concentrated rape honey, locust honey and chaste tree honey by using pyruvic acid |
| CN113933334B (en) * | 2021-10-13 | 2024-03-26 | 北京工商大学 | Locust honey authenticity identification method based on feature selection and machine learning algorithm |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104713895A (en) * | 2015-03-13 | 2015-06-17 | 中国科学院武汉物理与数学研究所 | Method for distinguishing between pure and syrup-adulterated honey based on combination of hydrogen nuclear magnetic resonance and partial least square method |
| CN105510372A (en) * | 2016-01-27 | 2016-04-20 | 江苏出入境检验检疫局动植物与食品检测中心 | Method for building model for DPLS-BS-UVE rapid honey identification |
| CN105548233A (en) * | 2015-10-30 | 2016-05-04 | 中国科学院武汉物理与数学研究所 | Method for discriminating acacia honey and rape honey on basis of H-nuclear magnetic resonance |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070055456A1 (en) * | 2005-08-31 | 2007-03-08 | Daniel Raftery | NMR method for differentiating complex mixtures |
-
2017
- 2017-08-25 CN CN201710742918.7A patent/CN107561110B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104713895A (en) * | 2015-03-13 | 2015-06-17 | 中国科学院武汉物理与数学研究所 | Method for distinguishing between pure and syrup-adulterated honey based on combination of hydrogen nuclear magnetic resonance and partial least square method |
| CN105548233A (en) * | 2015-10-30 | 2016-05-04 | 中国科学院武汉物理与数学研究所 | Method for discriminating acacia honey and rape honey on basis of H-nuclear magnetic resonance |
| CN105510372A (en) * | 2016-01-27 | 2016-04-20 | 江苏出入境检验检疫局动植物与食品检测中心 | Method for building model for DPLS-BS-UVE rapid honey identification |
Non-Patent Citations (1)
| Title |
|---|
| Origin Identification and Quantitative Analysis of Honeys by Nuclear Magnetic Resonance and Chemometric Techniques;Xin Zheng et al.;《Food Anal. Methods》;20151003;第9卷(第6期);1470-1479 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107561110A (en) | 2018-01-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107561110B (en) | A kind of discrimination method being used for chaste honey, rape honey and acacia honey based on nuclear magnetic resonance technique | |
| CN107300565B (en) | The NMR spectrum method of rice syrup is mixed in a kind of quick identification honey | |
| CN105510372B (en) | Set up the true and false model method of DPLS BS UVE quick discriminating honey | |
| CN104713895B (en) | Method for distinguishing between pure and syrup-adulterated honey based on combination of hydrogen nuclear magnetic resonance and partial least square method | |
| CN107561111B (en) | The NMR spectrum method of cassava syrup is mixed in a kind of quick identification honey | |
| Foxall et al. | Magic angle spinning proton nuclear magnetic resonance spectroscopic analysis of intact kidney tissue samples | |
| Huang et al. | A high-resolution 2D J-resolved NMR detection technique for metabolite analyses of biological samples | |
| CN107015003A (en) | It is a kind of to be used for the kit of quantitatively detection homocysteine, cysteine and methionine | |
| Dalvit et al. | 19F NMR transverse and longitudinal relaxation filter experiments for screening: a theoretical and experimental analysis | |
| CN108872289B (en) | Manuka honey identification method based on nuclear magnetic resonance technology | |
| JP2002536664A (en) | Processes for quality control and standardization of medicinal plant products | |
| Maiwald et al. | Quantitative on-line high-resolution NMR spectroscopy in process engineering applications | |
| Euceda et al. | NMR-based prostate cancer metabolomics | |
| Goodman et al. | High field NMR microscopic imaging of cultivated strawberry fruit | |
| CN108287206B (en) | Method for quantitatively detecting barbiturates in hair and application | |
| Bigler et al. | Improved impurity fingerprinting of heparin by high resolution 1H NMR spectroscopy | |
| Nagana Gowda et al. | One‐step analysis of major bile components in human bile using 1H NMR spectroscopy | |
| Gowda et al. | Isotope enhanced approaches in metabolomics | |
| Sheedy | Metabolite analysis of biological fluids and tissues by proton nuclear magnetic resonance spectroscopy | |
| Bataille et al. | NMR waterLOGSY as An Assay in Drug Development Programmes for Detecting Protein-Ligand Interactions–NMR waterLOGSY | |
| JP2005345193A (en) | Quantitative determination method using nuclear magnetic resonance method and/or diffusion factor measurement method based on nuclear magnetic resonance | |
| CN104655666A (en) | Low field nuclear magnetic resonance detection method of edible gelatin quality | |
| CN108614000A (en) | Manuka honey discrimination method based on SIMCA models | |
| KR20090042632A (en) | A quantitative analysis method of heteronuclear-materials by using nuclear magnetic resonance spectroscopy | |
| Dais | Nuclear magnetic resonance: methodologies and applications |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liu Yun Inventor after: Chen Lei Inventor after: Wu Bin Inventor after: Ding Tao Inventor after: Zhang Jian Inventor after: Fei Xiaoqing Inventor after: Zhang Xiaoyan Inventor after: Zhang Rui Inventor before: Liu Yun Inventor before: Zhang Rui Inventor before: Ding Tao Inventor before: Zhang Jian Inventor before: Fei Xiaoqing Inventor before: Chen Lei Inventor before: Zhang Xiaoyan Inventor before: Wu Bin |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190610 Address after: 210000 Building No. 1, No. 39 Chuangzhi Road, Jianye District, Nanjing City, Jiangsu Province Co-patentee after: Jiangsu Sinography Testing Co., Ltd. Patentee after: Propagation and Food Test Center of Jiangsu Entry-Exit Inspection and Quarantine Bureau Address before: Jiangsu Entry-Exit Inspection and Quarantine Bureau, No. 99 Zhonghua Road, Nanjing, Jiangsu Province, 210001 Patentee before: Propagation and Food Test Center of Jiangsu Entry-Exit Inspection and Quarantine Bureau |