CN107552114A - A kind of multichannel syphilis diagnosis micro flow control chip device - Google Patents

Abstract

The present invention relates to a kind of multichannel syphilis diagnosis micro flow control chip device, belong to analysis testing field.The substrate of controller used in syphilis diagnosis micro-fluidic chip is made with dimethyl silicone polymer i.e. PDMS cheap and easily process, there are some problems to need to solve.This case main points are, the selected PDMS with ecosystem surface of substrate, and fastening is positioned at the sample liquid stream terminal of the micro-fluidic chip its neighbor positions manually by the hinge-type fixture for being attached to miniature ultrasonic transducer units, with driving force of the ultrasonic wave induced synthesis based on interfacial tension difference, its driving force couples running with Micropump simultaneously, collaboration drives test liquid to flow to terminal direction flowing, micron silica granule filler is filled up in its pipeline of the chip, its function of the filler includes supporting pipeline inwall, and, with the set of hydrophily fluid channel come the hydrophobic pipeline of compensatory script, and, extruding, cover inner-walls of duct face and thereby reduce the chance that large biological molecule contacts with the internal face.

Description

A kind of multichannel syphilis diagnosis micro flow control chip device

Technical field

The present invention relates to a kind of multichannel syphilis diagnosis micro flow control chip device, the device is anti-based on antigen/antibody specificity The special purpose device of Diagnosis of Syphilis antigen should be carried out, belong to analysis testing field.

Background technology

The micro-fluidic controller used in syphilis diagnosis technical background of associated multi-channel, it may refer to the grade applications for a patent for invention of CN 200910152141.4 Case.

Only for the microflow control technique overall general picture of itself, it may refer to famous micro-fluidic expert Mr. Lin Ping Cheng and go out not long ago Monograph " diagram Microfluid based Lab on a chip ", the monograph is published via Science Press, and the monograph is to microflow control technique Past, now, and, vision of the future etc. etc., suffer from detail, be deep into long discussion of detail.

So, the Important Problems of this case that to have a talk below concern.

The basic framework of micro-fluidic chip, including it is etched with the substrate of conduit and the cover plate to fit together therewith, the substrate On fluid course, before upper cover plate is assembled, it is apparent on see to be exactly some conduits, wait until cover cover plate thereon after, Just really closure forms the fluid course, and the conduit inner surface of the conduit is together with the part cover plate structure that surround the conduit Into described fluid course；So, it is clear that assemble the fluid course after completing, the main portion of its inner surface area Point it is the inner surface area of that conduit, in other words, the state or property of the conduit inner surface substantially determine that the liquid stream is led to The integrality or property in road；Therefore say, the inner surface state or inner surface property of this conduit of the structure on substrate are crucial Factor；In principle, any material that can be kept or keep its solid forms substantially, can be used to make substrate and lid Piece, such as, the material that can act as substrate and cover plate can be for example poly- diformazan of monocrystalline silicon piece, quartz plate, sheet glass, high polymer Radical siloxane, polymethyl methacrylate, makrolon etc.；Certainly, the selection of substrate and the selection of cover plate can with identical, It can also differ；From the point of view of material consumption, manufacture difficulty and application popularization prospect etc. etc., exist between these materials Not small difference, the especially selection of that substrate, have a great influence.

In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, so Substrate on to make conduit extremely simple, and the lower cost for material, substrate is made with the polydimethyl siloxane material, Conduit is suppressed or etched thereon, and is engaged with the cover plate that the cheap material such as glass or polypropylene or other plastic sheets makes, seemingly It is a kind of more satisfactory selection；Certainly, patch material can also select to use cheap polydimethyl siloxane material：So, This substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, and making is extremely simple, seems and should also be as being extremely easy to Popularization, promote.

But thing is really not so simple.

First, this polydimethyl siloxane material, that is, the material that alphabetical PDMS is referred to of abridging, itself are a kind of strong Hydrophobic material, conduit is built on this material, if without being operated for the modified of the conduit surface, then, it is overall After assembling is completed, that is, after covering cover plate, because the conduit its inner surface in structure is occupied in most fluid course Surface, then, its strong hydrophobic property of the PDMS conduits inner surface, is deciding factor, and it can be similar to water-soluble The fine liquid stream of polar liquid of liquid by becoming very difficult, its flow resistance is big, or even in general Micropump is all difficult to promote, Certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar；Therefore, existing Among having technology, modification is modified particular for the conduit inner surface on the PDMS material, is necessary operation；So, This is pretty troublesome for the modified operation of PDMS conduit inner surfacesThat falls nor this problem, forms serious technical puzzlement , it is another problem：PDMS polymer molecules inside its body phase of this PDMS material substrate, which have from trend surface, to be expanded Dissipate, the characteristic of migration, the characteristic that PDMS polymer molecules are diffused to the surface, migrated automatically inside this substrate body phase, will make The sufficiently long time must can not be maintained by the state after the modification of its inner surface of that conduit of surface modifying and decorating, that Holding time for conduit its inner surface state after surface-modified is substantially only sufficient to the time for completing laboratory internal test experiments Need；In other words, by surface is modified or the PDMS conduit inner surfaces of surface modification, after it is modified or modification is said The surface state formed afterwards can not be lasting, but soon automatically tends to or say the surface state before becoming surface modification again, The strong hydrophobic surface state of that script is returned in the shorter time, then, just think, such micro-fluidic chip energy Enough a large amount of making, mass storages, it is widely popularized, answer is it is obvious that is, impossible.On this PDMS material Conduit, if not doing surface modification, it can not pump and pass through similar to the fine liquid stream of polar solvent of the aqueous solution, chip also just cannot Use；And if having done surface modification, its state after modifying can not be persistently kept again, or equally can not popularization and application.

According to the literature, there is a kind of expedient solution, be that a small amount of surfactant is added in sample to be tested solution, with Just during test, dynamic, temporary transient effective surface hydrophilic layer is built temporarily in the PDMS conduits inner surface；However, Due to the amphipathic characteristic of surfactant, the introduced surfactant of the program is inevitable simultaneously also can be with being tested in sample solution Combination occurs for composition so that tested composition can not be detected normally or it is detected ratio and dubiously reduced；Live on the surface Property agent is possibly even fully wrapped around by tested composition with micelle form so that tested composition can not be detected or identify；Therefore, should Kind adds the scheme of surfactant into sample solution, is far from a preferable solution.

So, how to accomplish that substrate can either be made using cheap PDMS material, and the conduit inner surface can be released Decorating state can not persistently, chip can not largely make, largely lay in and then be widely popularized and such a make the numerous specialties in this area The puzzlement that personnel are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.

Be present many year in the highly difficult problem, so far, not yet properly settled.

Second, the PDMS material of non-surface modification, it is stated that, its surface is strongly hydrophobic above, this strong hydrophobic Material surface and also another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and And these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into it is gradually deep, directly It is trapped within the body phase of PDMS substrates to heavy, in fact, this process, is partly also due to inside PDMS material body phase Polymer molecule, which has, to be diffused to the surface, caused by travel motion；Such case, it can also be explained from another angle, i.e. Continuously from inside PDMS body phases to those polymer molecules of its diffusion into the surface, migration, its result moved, be by Gradually those are involved within the body phase of PDMS substrates by the large biological molecule of adsorption, briefly, these are inhaled Attached large biological molecule is exactly to be swallowed up by PDMS substrate body phases；So, this PDMS substrates body phase swallows up large biological molecule Phenomenon, the influence caused by it, necessarily cause the severe deviations of all kinds of test data of experiment for being related to large biological molecule.

As described above, the problem of PDMS substrates, is, its not only adsorption large biological molecule, and swallow up large biological molecule, So, as the large biological molecule of experiment test object, its disappearance will not stop because surface saturation is adsorbed, but, no It is disconnected adsorbed, also constantly swallowed up.

On PDMS substrates in related experiment test process its body phase constantly swallow up test associated biomolecule macromolecular phenomenon, separately It is to say that one kind, which is explained, substantial amounts of Minute pores in PDMS body phases be present, and associated biomolecule macromolecular is by after adsorption, depression Into these Minute pores, and then swallowed up；However, inventor thinks, those can allow the air point of miniature scale Son squeezes into the Minute pores therebetween, not equal to saying that they also can directly allow the large biological molecule of relative large scale to enter, two Person's difference on yardstick is huge, must not make sweeping generalizations.Explanation is bypassed, in any case, the biology as dependence test analysis object Macromolecular is adsorbed by PDMS substrate conduits inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is known objective deposits Phenomenon.

, can be from containment PDMS surfaces to life in order to prevent swallow up effect of this PDMS substrate bodies relative to large biological molecule The absorption of thing macromolecular addresses, and method is chemically modified modification aiming at the PDMS material surface, for For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described channel portion, by chemistry The conduit inner surface of modification, can contain its absorption to large biological molecule, and then avoid large biological molecule quilt PDMS substrate body phases are swallowed up；But or that old problem, that is, the chemical modification on PDMS material surface changes Surface state after property can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface automatically, The process of migration, soon it can become that conduit inner surface state being modified by surface chemical modification again script strong hydrophobic And the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS bases Its conduit inner surface of piece is always rapidly to strong hydrophobic surface state evolution.

So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, and can enough reaches Growth stage contains the absorption process of the PDMS substrate conduit inner surfaces to large biological molecule, and then prevents PDMS substrate bodies relative The effect of swallowing up of large biological molecule so that related chip manufactured goods are able to maintain that a prolonged enough, rational shelf-life, It is exactly a very intractable problem.The problem equally makes the numerous specialties in this area as another problem addressed above Personnel are entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.The hardly possible Also be present many year in topic, so far, also not yet properly settled.

Third, as described above, the PDMS conduit inner surfaces are strongly hydrophobic, and targetedly surface chemical modification or surface chemistry Modification is difficult to persistently again, therefore, actually can only still belong to effective short-term in the surface state after the modification of its surface or surface modification Within use it；If having had been subjected to that ofer short duration period of validity, and still by force if use, due to surface state Bigger flow resistance is then already certainly existed using usual Micropump driving sample liquid stream close to hydrophobic state again, this Sample, just by increasing Micropump pump power and pumping pressure test liquid can only be forced to flow to target direction flowing, as described above, this Kind of PDMS material is soft, pumps sample liquid stream with too high, mechanical pumping pressure, will cause its sample introduction of the substrate Bubbling, expanded, distortion, deformation occur for the conduit that end includes sample introduction end near zone, also, under this high-pressure situations, Under the sample introduction end and its conduit of near zone and its periphery also easily stripping between generation substrate and cover plate, this situation, Sample solution flows in the crack for entering appearance of the stripping afterwards between the substrate and cover plate of formation everywhere, and this is actually resulted in The damage of the micro-fluidic chip；Certainly, it is not in place if surface modification or surface modification script, also result in of short duration There is said circumstances within the usual term of validity；In the case where carrying out liquid stream driving using additional Micropump merely, the above-mentioned problem All the time exist.As described above, if the pre actions such as any surface modification or surface modification were not done completely, then, The above-mentioned problem will be even more serious, even without conduit bubbling, expanded, distortion, change described in generation sample introduction end and its near zone The problems such as being peeled off between shape and substrate and cover plate, merely because the flow resistance is excessive, it be able to may not also be driven using high pressure Micropump Dynamic sample liquid stream is marched forward towards terminal side.

Fourth, the polydimethyl siloxane material, namely PDMS material, the active force very little wherein between polymer molecule, its The polymer molecule of low polymerization degree inside body phase is in constantly into the dynamic process of surface migration, therefore, includes PDMS bases After it has been formed, the surface topography in its inner-walls of duct face of chip can be because of described low polymerization degree polymer molecule not for the chip of sheet material Migrate disconnectedly, pour into the surface and change, the change of its pattern is somewhat similarly to Rheological Deformation, trickling deformation or deformation of creep institute Pattern changes caused by possible；In the higher PDMS material of some low polymerization degree component ratios, this kind is somewhat similarly to flow The phenomenon of deformation, trickling deformation or the deformation of creep can show must be than more significant；Its shaping of chip of this kind comprising PDMS substrates The dynamic of inner-walls of duct surface topography afterwards changes property, namely the described property for being somewhat similarly to Rheological Deformation or trickling deformation Property or the deformation of creep property, this case applies mechanically the word of rheology one, integration, also referred to as the rheological equationm of state or Rheological Deformation property, The dynamic of corresponding inner-walls of duct face pattern changes, and this case is also referred to as rheology；As described above, because this kind of material its own The certain Rheological Deformation property as described above having, its manufactured goods actually can at leisure Rheological Deformation and cause the manufactured goods whole Individual structural form changes；Influence caused by its Rheological Deformation property, in terms of macroscopic perspective be it is subtle, still, For the very fine micro-fluidic chip of its internal structure, the rheological equationm of state can but cause bigger influence, therefore, in advance The fluid course namely the pipeline formed when first making, because the wall of its tube chamber of pipeline is mainly PDMS materials Wall, so, the cross-sectional area of the pipeline can actually taper into because of rheology over time, in other words, Due to the rheology, can more it become more for natively finer its tube chamber of the pipeline that liquid sample flows through Narrow, in the case of flow time is sufficiently long, or even the tube chamber of the pipeline can partly be closed or fully closed, and then be caused Sample fluid course is thoroughly blocked, the journey for closing or fully closing with being even also not reaching to its luminal part of pipeline Degree, its fluid passage that can be provided of that pipeline narrow because of rheology, then, in pipeline, its inner surface is exactly originally strong It is strong it is hydrophobic in the case of, aqueous sample liquid is just more difficult to by becoming narrower micro-channel so because of rheology, then, The influence of substrate rheology how is resisted, maintains the basic framework of the pipeline that gross distortion does not occur for a long time, also, keep the pipeline Long-term unobstructed, the problem of being exactly a urgent need to resolve.

The content of the invention

The technical problem to be solved by the invention is to provide a package solution, while solves the system addressed above The problem of row, also, the solution is applied to controller used in syphilis diagnosis multichannel micro-fluidic chip field, formed a kind of new Controller used in syphilis diagnosis Multichannel device.

The present invention solves the technical problem by following scheme, and the device that the program provides is that a kind of multichannel syphilis diagnosis is used Micro flow control chip device, the structure of the device include multichannel micro-fluidic chip, and the structure of the micro-fluidic chip includes being bonded to each other Installing substrate and cover plate together, the substrate and cover plate are plate object or tablet, the substrate towards the cover plate that The channel structure via mould pressing process or etching technics formation is contained in individual face, is bonded to each other the substrate and the cover plate being installed together The micro-fluidic chip containing pipeline configuration has been built into jointly, and the locations of structures of the pipeline is located at the substrate and is bonded to each other with the cover plate Juncture area, the both ends of the pipeline are connected with the sample introduction end of the micro-fluidic chip and terminal respectively, and the sample introduction end is the miniflow The injection end of chip sample solution is controlled, the terminal is sample solution flowing in its chip when the actual sample introduction of the micro-fluidic chip is tested Terminal, the terminal are located remotely from each other with the sample introduction end, the distance between the terminal and the sample introduction end between 3 centimetres with 10 centimetres it Between, working electrode is sequentially equiped with diverse location in the pipeline and to electrode and reference electrode, the working electrode by Conductive electrode and the gold size sensitive membrane for having embedded syphilis specific antibody being attached on the conductive electrode are formed, the pipeline Construction be in parallel construction, the pipeline in parallel construction is made up of four lateral parallel connections, the quantity of the working electrode It is four, the installation position of four working electrodes is located in four laterals respectively, and, four working electrodes Specific antibody in its top layer gold size sensitivity membrane structure is the four kinds of syphilis antibody things that can be specifically bound to Treponema pallidum antigen respectively Matter, four kinds of antibody materials are syphilis specific antibody TP0684, TP0453, TP0821 and TP0319 respectively, the work Its material of electrode is gold material or thermal decomposition conducting polymer material, and sheet or thread, weight is presented in its pattern of the working electrode Point is that its material of the substrate is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, the primary form Surface its be intended to refer to the primary form of the not material by any surface chemical modification or any surface chemical modification Surface, the structure of the device also includes hinge-type fixture, hinge-type fixture its appearance profile likeness in form hinge, hinge-type folder Tool is by two hinges being mutually hinged and through a fastening screw of two hinges and one and the fastening spiral shell Silk matching is simultaneously formed with what the fastening screw connected together for the nut of fastening and hand break loose manually, the hinge-type fixture Two hinges are respectively mutually drawn close with its tip and clamp the micro-fluidic chip, and the tip is positioned at the neighbouring institute of the micro-fluidic chip State in the locations of structures of terminal, fixation is attached in a hinge at least in and is equiped with miniature ultrasonic transducer units, with And higher-order of oscillation electric signal transmission cable, one end and the miniature ultrasonic transducer units of the higher-order of oscillation electric signal transmission cable connect It is connected together；The hinge-type fixture provides a function of facilitating the device to disassemble；Its main work(of the miniature ultrasonic transducer units It can be in the actual sample introduction test of micro-fluidic chip, be filled using the sample introduction end and the terminal with the miniature ultrasonic transducer units If the difference on the distance between position difference and its ultrasonic intensity experienced, its interface of sample introduction end described in induced synthesis Difference between tension force and the terminal its interfacial tension, the interfacial tension difference between the micro-fluidic chip both ends can be micro- at this Pressure gap is formed between the both ends of fluidic chip, the pressure gap can drive sample solution to the end flow；Softness is simultaneously Its function of the substrate of the dimethyl silicone polymer material of tool elasticity is included with its property to the strong absorption of ultrasonic wave, to ultrasonic wave Absorbed strongly, and ultrasound is thereby realized within micro-fluidic chip terminal to the limited short distance between the sample introduction end The rapid decrement of intensity of wave；And Micropump, the Micropump are connected with the sample introduction end, the Micropump is built-in Micropump or external micro- Pump；The function of the Micropump is the interface come with the mechanicalness pumping strength of the Micropump between the both ends of ultrasonic wave induction Its caused driving force of tension difference is supported mutually, adjusts mutually, is mutually coupled, and one is pooled in a manner of Collaboration Stock driving test liquid flows to the strength of the terminal direction flowing；Described its thickness of cover plate and substrate is between 1.0 millimeters and 5.0 Between millimeter；And many silica dioxide granules, many silica dioxide granules are filled in the pipeline, the pipe Its tube chamber of road is filled up by many silica dioxide granules, and its particle size range of the silica dioxide granule is between 10 microns and 200 Between micron, the silica dioxide granule is silica crystalline particles, silica glass granule or silica composition shared by it Inorganic glass particles of the percentage by weight more than 80%.

Its particle diameter of the silica dioxide granule can be that the basis between 10 microns and 200 microns is actually needed and is arbitrarily designated Particle diameter, such as 10 microns, 30 microns, 70 microns, 100 microns, 150 microns or 200 microns of the particle diameter, etc..

The scope of further preferred its particle diameter of the silica dioxide granule is between 100 microns and 200 microns.

Its pattern of the silica dioxide granule is unlimited, and the silica dioxide granule for example can be spheric granules, rod-shaped particles, cuboid Shape particle or any amorphous granular.

The various silica dioxide granules manufacturing technology of itself is known technology.

Various forms, the silica dioxide granule market of all size are on sale.

The silica dioxide granule can also customize to specialized factory.

The preferred scope of its internal diameter of pipeline is between 500 microns and 1000 microns；It is but preferred compared to above-mentioned Internal diameter of the pipeline scope, more tiny internal diameter of the pipeline or thicker internal diameter of the pipeline are also what this case was allowed.

Described miniature ultrasonic transducer units can certainly be all installed in two hinges；But only installing one is micro- Type ultrasonic transducer is dealt with enough to be used.

The word of hinge one art-recognized meanings of itself are known.

The word of Micropump one art-recognized meanings of itself are known for the professional in micro-fluidic chip field.

For example miniature piezoelectric pump of the Micropump, miniature peristaltic pump or miniature air driven pump.

The Micropump both can be the Micropump of external form；The Micropump can also be done into or say inside the embedded micro-fluidic chip The Micropump of the built in version of its sample introduction end structure position or the sample introduction end Near-neighbor Structure position.

The gold size sensitive membrane is to be sufficiently mixed chitosan gold size solution and syphilis specific antibody-solutions uniformly, uses point sample instrument Point sample is coated on specified structure position, and forms its drying and forming-film.Syphilis specific antibody in the gold size sensitive membrane is equal The syphilis antibody marked for horseradish peroxidase or glucose oxidase, the gold size sensitive membrane have been included as fixing above-mentioned each Syphilis specific antibody and introduce complementary medium therein, the complementary medium such as chitosan, cellulose acetate, gelatin Therein a kind of or their mixture.

The pipeline in the microfluidic chip structure includes the lateral, and its internal diameter size may each be arbitrarily selected Size, still, for prepare liquid sample and the consideration of reagent loss etc. is reduced less as far as possible, the pipeline includes described Lateral preferably selects the passage of capillary level, and the passage of the capillary level implies that its internal diameter and the capillary on ordinary meaning The suitable passage of the internal diameter of pipe.The shape of cross section of its inner passage of capillary can be arbitrary shape, the cross section Shape is for example circular, oval, square, rectangle, bar shaped, naturally it is also possible to be arbitrarily exist bending it is linear, also, With the extension of pipeline, the shape of cross section of different parts can also allow to be different shapes the interior shape of the capillary. Only for the word of capillary one, its art-recognized meanings is known.

What is be related in structure is the electrode of microsize to electrode and reference electrode, and its electrode shape may each be any choosing Fixed shape, the arbitrarily selected shape such as square piece shape, rectangular patch, strip or round sheet etc..It is described to electricity The art-recognized meanings of pole and the reference electrode vocabulary of itself are known.

It is public only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field Know.

Various sizes, variously-shaped ultrasonic transducer are commercially available；Commercially available miniature ultrasonic transducer units its sizes can be with It is small to arrive the magnitude only calculated with millimeter.

Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface itself and Speech, is known general technology for the professional in ultrasonic technology field.This case is not to this expansion superfluous words.

Only with regard to naked PDMS substrates itself conduit molding or lithographic technique for, be open-and-shut known technology.

The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.

The structure of the device can also include higher-order of oscillation electric signal generator；Its is another for the higher-order of oscillation electric signal transmission cable One end can be connected with the higher-order of oscillation electric signal generator.

The involved higher-order of oscillation electric signal generator technology of itself, for the professional in ultrasonic technology field, it is It is simple and known；The higher-order of oscillation electric signal generator can customize to ultrasonic instrument specialized factory.

The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units be between 5 milliwatts and 9000 milliwatts it Between；The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with Between 12MHz.

This case device can further include some annexes, the annex such as multiple tracks electrochemical workstation etc., institute certainly The art-recognized meanings for stating multiple tracks electrochemical workstation are known.Each working electrode for being related in this case microfluidic chip structure and , can be respectively via corresponding special the corresponding interface got lines crossed with the multiple tracks electrochemical workstation to electrode and reference electrode etc. Coupled.It is described that special to get lines crossed be for each the corresponding interface of each electrode and the multiple tracks electrochemical workstation is carried out into phase The private cable being mutually coupled with.The micro-fluidic chip in this case device, its structure can also include micro-valve, the number of the micro-valve Measure unlimited, according to being actually needed, the micro-valve can be installed in the position of any required installation in the microfluidic chip structure； For the word of micro-valve one for the professional of micro fluidic chip technical field, the art-recognized meanings of itself are known；The micro-valve The manufacturing technology of itself and the use of technology is also known；The component that the micro-valve is not required.

The diameter of the working electrode can allow to be that any setting is easily installed the suitable diameter used, it is however recommended to Or say preferable its scope of the diameter between 0.1 micron to 2000 microns；The length of the working electrode can permit Permitted to be that any setting is easily installed the length used, it is however recommended to or to say the preferable length its scope be at 1 micron To between 15000 microns.

The gold size that the working electrode surface layer is installed in by spraying or point sample instrument point sample or the coating of other appropriate process is quick Feel film, its thicknesses of layers can allow be any setting treat sample measuring liquid occur electrical signals response thickness, still, push away Preferable thickness is between 10 nanometers and 200 nanometers to the thickness recommended in other words.

The cover plate in chip structure, its material can allow to be any electrical insulating property material, such as：Polypropylene, glass Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, for example do Into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and realized in the extremely short distance to ultrasonic wave Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip Using dimethyl silicone polymer it is used as the cover plate, and this case technical scheme is allowed.

Described its thickness of cover plate and substrate can allow be any setting be easy to assembling thickness, the thickness of recommendation or say preferably Thickness be between 1.0 millimeters and 5.0 millimeters.Less thickness is advantageous to save material.

The application method of this case micro-fluidic chip：

This case is flowed with dual drive coupling operating mode driving liquid stream in the capillary channel of the four-way micro-fluidic chip, Four kinds of controller used in syphilis diagnosis antigens are detected respectively using four-way electrochemical analytical instrument.

The specific detection of this case micro-fluidic chip is as follows using step：

1st, blood serum sample liquid is added in micro-pipe road, under dual drive coupling operating mode driving, various Treponema pallidum antigens point The syphilis specific antibody for the corresponding horseradish peroxidase-labeled that son is embedded by gold size sensitive membrane on electrode surface in each passage Capture.

2nd, the syphilis specific antibody of horseradish peroxidase-labeled forms immune complex with the Treponema pallidum antigen in blood serum sample.

3rd, using multi-channel electrochemical analyzer, the electron mediators such as catechol are added, using the above-mentioned reaction of amperometric detection Caused curent change, it is derived from the species and content of various analytes.

4th, result is subjected to comprehensive analysis, comprehensive diagnos is carried out to Treponema pallidum antigen.

It is an advantage of the invention that its close position positions the hinge-type fixture in the terminal of the micro-fluidic chip, and In the hinge-type fixture, its hinge attaches installation miniature ultrasonic transducer units, with miniature ultrasonic transducer units transmitting low-power, The ultrasonic wave of high-frequency band, meanwhile, using its strong absorbability to ultrasonic wave of dimethyl silicone polymer substrate, shorter Distance in, it is, in from the terminal to the very short distance of only several centimeters yardsticks the sample introduction end, reach super The rapid decrement of intensity of acoustic wave, the difference of the interfacial tension is thereby caused at the both ends of the micro-fluidic chip, this kind is because super Acoustic irradiation and the pressure gap that is formed itself is exactly one driving strength of sample solution to the end flow, while it is also Intercoupled with the driving force that Micropump is provided, Collaboration, above-mentioned two bursts of strength makes a concerted effort driving sample liquid stream described in the lump Flowed in pipeline to the terminal direction.

Its scheme of this case and by the pipeline except electrode installation take up space in addition to all clearance spaces all use described in Silica dioxide granule fills up.Those are filled in the silica dioxide granule in pipeline, and this case is filled out to its overall abbreviation silica dioxide granule Fill thing, its function includes support, withstands the tube chamber of the pipeline its inwall so that the tube chamber of the pipeline from because of the rheology and Narrow, prevent its reduced cross-sectional area of the tube chamber of the pipeline or even close, so as to maintain the basic framework of the pipeline not send out for a long time Raw serious deformation.

Numerous silica dioxide granule entities are mutually banked up under this case framework together at random in the pipeline, the silica The function of grain filler, also includes with the hydrophilic surface of its silica dioxide granule, utilizes each adjacent silicon dioxide particle certainly Mutual formed complications are continuous and its inner surface that is mutually close together by water-wetted surface has the sky of hydrophilic nmature Gap, in the hydrophilic fluid channel of the pipeline Inner Constitution network morphology, the having all the time although this complications can be continuous The fluid channel of hydrophilic nmature, then, inside the tube chamber of the pipeline simultaneously and a plurality of fluid channel deposited its synthesis, The effect of integrated, cumulative superposition in other words, is the equal of the relatively fine hydrophilic capillary channel of caliber；The complications Its presence of continuous hydrophilic fluid channel, counteracts its surface of the PDMS substrates of the primary form to a large extent The flow resistance for liquid sample caused by strong hydrophobic property；In other words, because of liquid sample its account for maximum ratio into It is water to divide, and liquid sample is substantially exactly the aqueous solution, and therefore, the presence of the silica dioxide granule filler can be significantly Overcome with the unmodified PDMS substrates of hydrophobic property and surface its to the aqueous solution it is incompatible, repel and barrier effect, And then be greatly reduced belong to properties of Aqueous Solution sample liquid stream its be advanced through the resistance of pipeline.

Under this case framework, inside the tube chamber of the pipeline, the area of its lumen wall wall of former pipeline, because of the titanium dioxide The rigid of silicon grain filler ties up, on lumen wall wall still can also be exposed its face of the surface of part hydrophobic property Product is already dramatically reduced, and the area on its still exposed part surface for belonging to hydrophobic property does not have far smaller than The wall surface area of the hydrophobic property of the lumen wall wall in the presence of silica dioxide granule filler；So, at this Under the case framework, in its lumen of the pipeline, its remaining hydrophobic surface increases surface newly with hydrophilic part, that it is superimposed, comprehensive , summation, cumulative technique effect, be to form the surface for tending to be hydrophilic on the whole；In other words, in the pipeline Inside tube chamber, its interfacial tension between sample solution of the part surface of remaining hydrophobic property, plus that of hydrophilic nmature Newly-increased its interfacial tension between sample solution of surface in part, that it is superimposed, comprehensive, summation, cumulative technique effect, Be so that its solid-liquid interfacial tension inside the tube chamber of the pipeline synergy level off to its tube chamber inner surface of capillary glass tube with Interfacial tension between sample solution.

On the other hand, due to its rheological equationms of state of the PDMS as substrate material, it is filled in the pipeline The silica dioxide granule filler, can gradually by constantly Rheological Deformation and to arest neighbors free space promote PDMS The pipeline wall of material more tightly wraps up, and this process eventually causes those part dioxies for pressing close to the lumen wall wall Silicon carbide particle is stuck in original place or is stuck in original place or embedding in-situ silica dioxide granule and remaining embedded in original place, the part The silica dioxide granule banked up with being mutually closely packed together each other incarceration together, due to this reason, the titanium dioxide Silicon grain filler just will not move in the pipeline easily, and silica dioxide granule therein, which is also substantially all, is locked in original Locate, therefore the stream framework of the micro-fluidic chip can be kept for a long time.

The particle size range of its particle of this case silica dioxide granule filler is between 10 microns and 200 microns, compared to general For organic molecule, general large biological molecule, the particle diameter of its particle of this case silica dioxide granule filler can be rated as it is huge, by In its huge particle diameter, and the inner surface of the surface of its polarity and the pipeline of strong hydrophobic PDMS materials can not be compatible Or say and mutually melt, then, the huge silica dioxide granule of this kind of particle diameter can not be by the interior table of the pipeline of PDMS materials Face is adsorbed, and can not more be swallowed up by the inner surface of the pipeline of PDMS materials, the huge titanium dioxide of this kind of particle diameter Silicon grain surely not be fully sunk in easily or sink to completely PDMS materials its among intrinsic many Minute pores, and And because above it is stated that the rheology the reason for, the silica dioxide granule filler can be stuck in original place in other words it is embedding In original place without moving easily.

Inside the tube chamber of the pipeline, its lumen wall wall of former pipeline is entirely the surface of hydrophobic property originally, as described above, Under this case framework, rigid because of the silica dioxide granule filler ties up, still can also be exposed on lumen wall wall Its area of the surface of part hydrophobic property be already dramatically reduced, its still exposed part for belonging to hydrophobic property The area on surface be far smaller than no silica dioxide granule filler in the presence of the lumen wall wall dredge The wall surface area of aqueous nature；The reason for because of the rheology, it is close to those silica dioxide granules of lumen wall wall, gradually Ground is partially embedded into or is partly absorbed among the lumen wall wall, its direction pipe of the silica dioxide granule among the state The part surface of cavity wall wall is with having occurred and that the lumen wall wall of matching deformation is brought into close contact, then, in tube chamber The part wall that the matching is deformed and is brought into close contact with silica particles of having occurred and that in wall wall is actually It can not be contacted with flowing through the sample solution of pipeline, that is to say, that the wall of this part can not be to flowing through the examination of pipeline Sample solution large biological molecule therein and organic molecule produce suction-operated.Based on above-mentioned mechanism, then because filling up described Its presence of the silica dioxide granule filler of pipeline, the overwhelming majority of its lumen wall wall of the pipeline are tightly hidden Lid, these described walls tightly covered can not contact with flowing through the sample solution of the pipeline, be only remainder The hydrophobic surface for not being brought into close contact by its surface of silica dioxide granule, tightly covering of fraction may be connect with sample solution Touch, in other words, under this case framework, large biological molecule and organic molecule in sample solution its with hydrophobic PDMS walls Direct touch opportunity significantly reduce, thus, the lumen wall faces of PDMS materials its to large biological molecule and organic molecule Adsorptive hindrance and swallow up interference be greatly reduced；As described above, this case scheme is advantageous to exclude or weakens the absorption Interference is disturbed and swallowed up, is advantageous to be lifted the reliability of correlation analysis test data.

As described above, this case silica dioxide granule filler, its function actually includes being similar to the pipeline for PDMS materials Inner surface carries out the effect of chemical modification, and functioning as its this respect of this case is by the inner surface of pipeline of the PDMS materials By the surface modification of hydrophobic property into the surface of hydrophilic nmature, still, with general, usual PDMS surface chemical modifications Its hydrophilic chemical decorative layer retention time is short, hydrophilic effect can not keep that situation prolonged enough is different, and particle described in this case is huge Big silica dioxide granule filler, its particle being stuck by rheology can not both be moved easily, the particle of its huge particle diameter It can not more be swallowed up easily by the inner-walls of duct of PDMS materials, therefore, its from this respect is similar to surface chemical modification Seen on technique effect, its technique effect of this case, be form it is permanent, can not erase, can not consume, not be corroded, not by Hydrophilic surface reforming layer swallowing up, being not dissolved, it is just comparable to a kind of in its correlation of PDMS base materials in effect The permanent hydrophilic modifying superficial layer built on surface.

With the simultaneous Micropump of miniature ultrasonic transducer units in its structure of micro-fluidic chip, its function is, with the Micropump Interfacial tension difference its caused driving force for coming between the both ends of ultrasonic wave induction of mechanicalness pumping strength Support mutually, adjust mutually, be mutually coupled, one driving test liquid is pooled in a manner of Collaboration and flows to the terminal side To the strength of flowing；The presence of the Micropump so that its ultrasonic wave emissive porwer of the miniature ultrasonic transducer units can be allowed to appropriateness Reduce, this is particularly suitable for for the situation containing ultrasonic wave sensitive composition in detection object；And due to the miniature ultrasonic transducer units And its presence of radiated ultrasonic wave, the micro-fluidic chip its both ends interfacial tension can be induced and difference occur and thereby bring spy Other driving force for sample solution, meanwhile, due also to being filled with its pipeline of this case micro-fluidic chip with hydrophily The silica dioxide granule of matter, then, in the scenario above, its flow resistance in the pipeline of sample liquid stream is greatly reduced, phase Ying Di, its running resistance of the Micropump are greatly reduced, so, the Micropump just can with than relatively low pumping pressure to be directed to The pumping work of sample solution is stated, because mechanical pumping pressure is greatly reduced, therefore, in such a situation, just not The conduit of the sample introduction end and its near zone caused by Yi Fashengyin sample introductions end its mechanicalness pumping pressure of Micropump is excessive Between bubbling, expansion, deformation, distortion and the region substrate and cover plate the problems such as stripping etc.；This case dual drive coupling running Scheme and add for the sample fluid flow action it is handling, can allow for using ultrasonic intensity, ultrasonic wave frequency Rate, Micropump pump power, the Micropump multiple indexs of pumping pressure etc. come be directed to the flow rate of the flowing, flowing action including to Preceding flowing or pause flowing or acceleration flowing etc. flowing action carry out multi-parameter and accurately manipulated；As described above, the presence of the Micropump, Its ultrasonic wave emissive porwer of the miniature ultrasonic transducer units is enabled to be allowed to appropriate reduction, then, the feature additionally aids guarantor The working electrode its sensitive coating is protected, is allowed to from ultrasound injury.

The technical scheme of this case dissolved totally address above to dimethyl silicone polymer substrate its apply related system Row technical barrier.Based on this case scheme, very cheap and easily processing and fabricating the polydimethyl siloxane material of this kind just has can Can in the micro-fluidic chip its prepare, production, using etc. field play bigger effect.

The miniature ultrasonic transducer units have been installed in fixation in the hinge-type fixture its hinge in this case structure, and the structure provides One facilitates the function that the device is disassembled, in this way, the hinge-type fixture is together with appended miniature ultrasonic transducer units in its hinge Just easily can be mutually disengaged with the micro-fluidic chip, then, the component that the part can freely depart from just can circulate benignly ground Reuse many times；The architectural feature is advantageous to save the use cost of the device.

Brief description of the drawings

Fig. 1 is its rough outside side view of this case micro flow control chip device.

In figure, 1 is hinge-type fixture, and 2 sign linkwork positions, 3 be fastening screw, and 4 be higher-order of oscillation telecommunication Number transmission cable, 5 be miniature ultrasonic transducer units, and 6,11 be two different hinges of locations of structures respectively, and 7 be cover plate, 8 It is substrate, 9 be sample introduction end, and 10 be terminal, and 12 be nut, and 13 be infusion tube, and 14 be Micropump；The conjunction in legend Page clamp structure is only the legend structure of signal, and actual its structure of the hinge-type fixture is not limited to the legend hinge-type fixture knot Structure；Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, its sample liquid stream Flow direction.

Embodiment

In this case that Fig. 1 is shown embodiment, the structure of the device includes multichannel micro-fluidic chip, the micro-fluidic core The structure of piece includes being bonded to each other the substrate 8 and cover plate 7 of installing together, and the substrate 8 and cover plate 7 are plate object or piece The channel structure via mould pressing process or etching technics formation, phase are contained in shape thing, that face towards the cover plate 7 of the substrate 8 The substrate 8 being mutually jointly mounted to together has been built into the micro-fluidic chip containing pipeline configuration jointly with the cover plate 7, the pipeline Locations of structures be located at the juncture area that the substrate 8 and the cover plate 7 are bonded to each other, the both ends of the pipeline respectively with the micro-fluidic core The sample introduction end 9 of piece and terminal 10 connect, and the sample introduction end 9 is the injection end of the micro-fluidic chip sample solution, the terminal 10 It is the terminal of sample solution flowing in its chip when the actual sample introduction of the micro-fluidic chip is tested, the terminal 10 and the phase of sample introduction end 9 Mutually remote, the distance between the terminal 10 and the sample introduction end 9 are between 3 centimetres and 10 centimetres, the different positions in the pipeline Put and be sequentially equiped with working electrode and to electrode and reference electrode, the working electrode is by conductive electrode and is attached to The gold size sensitive membrane for having embedded syphilis specific antibody on the conductive electrode is formed, and the construction of the pipeline is in parallel construction, institute State and be made up of in the pipeline of parallel construction four lateral parallel connections, the quantity of the working electrode is four, this four work electricity The installation position of pole is located in four laterals respectively, and, its top layer gold size sensitivity membrane structure of four working electrodes In specific antibody be the four kinds of syphilis antibody materials that can be specifically bound to Treponema pallidum antigen respectively, four kinds of antibody materials difference Syphilis specific antibody TP0684, TP0453, TP0821 and TP0319, its material of the working electrode be gold material or Conducting polymer material is thermally decomposed, sheet or thread is presented in the working electrode its pattern, and emphasis is, its material of the substrate 8 is Dimethyl silicone polymer material, its surface of substrate 8 are the surfaces of primary form, the surface of the primary form its be intended to refer to Not by any surface chemical modification or the surface of the primary form of the material of any surface chemical modification, the structure of the device Also include hinge-type fixture 1, its appearance profile likeness in form hinge of hinge-type fixture 1, the hinge-type fixture 1 is by being hinged Two hinges 6,11 together and through a fastening screw 3 of two hinges 6,11 and one and the fastening spiral shell The matching of silk 3 is simultaneously formed, the nut with what the fastening screw 3 connected together for the nut 12 of fastening and hand break loose manually 12 employ be easier manually operated butterfly nut in this example, and butterfly nut is commercially available, the hinge-type fixture 1 Two hinges 6,11 are respectively mutually drawn close with its tip and clamp the micro-fluidic chip, and the tip is positioned at the micro-fluidic chip Fixation, which is attached, in the locations of structures of the neighbouring terminal 10, in a hinge at least in is equiped with miniature ultrasonic Transducer, in this example, the miniature ultrasonic transducer units 5 are to attach to be fixed in hinge 6, and, higher-order of oscillation electric signal passes Transmission cable 4, one end of the higher-order of oscillation electric signal transmission cable 4 link together with the miniature ultrasonic transducer units 5；The conjunction Page fixture 1 provides a function of facilitating the device to disassemble；Its major function of the miniature ultrasonic transducer units 5 is in miniflow When the actual sample introduction of control chip is tested, installed using the sample introduction end 9 and the terminal 10 with the miniature ultrasonic transducer units 5 Difference in the distance between position difference and its ultrasonic intensity experienced, its interface of sample introduction end 9 described in induced synthesis Difference between tension force and the terminal 10 its interfacial tension, the interfacial tension between the micro-fluidic chip both ends 9,10 are poor Different to form pressure gap between the both ends 9,10 of the micro-fluidic chip, the pressure gap can drive sample solution to described Flow in the direction of terminal 10；It is soft and have the substrate 8 of the dimethyl silicone polymer material of elasticity its function and include with it to ultrasound The property that ripple absorbs strongly, ultrasonic wave is absorbed strongly, and thereby in the micro-fluidic chip, the terminal 10 arrives the sample introduction end The rapid decrement of ultrasonic intensity is realized within limited short distance between 9；And Micropump 14, the Micropump 14 enter with this Sample end 9 connects；The function of the Micropump 14 is to come the institute with ultrasonic wave induction with the mechanicalness pumping strength of the Micropump 14 The interfacial tension difference between both ends 9,10 its caused driving force is stated to support mutually, adjust mutually, be mutually coupled, with The mode of Collaboration pools the strength that one driving test liquid flows to the direction of terminal 10 flowing；The cover plate 7 and base Its thickness of piece 8 is between 1.0 millimeters and 5.0 millimeters；And many silica dioxide granules, many titanium dioxides Silicon grain is filled in the pipeline, and its tube chamber of the pipeline is filled up by many silica dioxide granules, the silica For its particle size range of grain between 10 microns and 200 microns, the silica dioxide granule is silica crystalline particles, titanium dioxide Silica glass particle or silica composition its inorganic glass particles of shared percentage by weight more than 80%.

Arrow in legend indicate the micro-fluidic chip its in actual motion, by both ends 9,10 pressure differentials drive, its The flow direction of sample liquid stream.

Fig. 1 is depicted without the associate members such as the higher-order of oscillation electric signal generator.

The Micropump 14 can customize to specialized factory.

Its connected mode with various micro-fluidic chip of various Micropump is known.

Described its main body of hinge-type fixture can simply be changed a social system using commercially available hinge；It can also be customized to hardware processing factory.

Involved miniature ultrasonic transducer units 5 are commercially available；It can also be customized to ultrasonic transducer producer.

Involved higher-order of oscillation electric signal transmission cable 4 is commercially available；Can also be to ultrasonic transducer producer or cable special manufacturer Family's customization.

Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available；It can also be customized to relevant manufacturers.

The Micropump both can be the Micropump of external form；The Micropump can also be done into or say inside the embedded micro-fluidic chip The Micropump of the built in version of its sample introduction end structure position or the sample introduction end Near-neighbor Structure position, its basic structure key element and external form Micropump it is identical.Micropump in legend is the Micropump of external form.

Each working electrode in this example structure and electrode and reference electrode via each special cable or can be said respectively Get lines crossed respectively with the corresponding cable interface of the multiple tracks electrochemical workstation as annex or saying interface connection of getting lines crossed.

Claims (10)

1. a kind of multichannel syphilis diagnosis micro flow control chip device, the structure of the device includes multichannel micro-fluidic chip, and this is micro- The structure of fluidic chip includes being bonded to each other the substrate and cover plate of installing together, and the substrate and cover plate are plate object or sheet Thing, that face towards the cover plate of the substrate are contained the channel structure via mould pressing process or etching technics formation, are bonded to each other The substrate being installed together has been built into the micro-fluidic chip containing pipeline configuration, the locations of structures of the pipeline jointly with the cover plate The juncture area being bonded to each other positioned at the substrate and the cover plate, the both ends of the pipeline respectively with the sample introduction end of the micro-fluidic chip and Terminal connects, and the sample introduction end is the injection end of the micro-fluidic chip sample solution, and the terminal is that the actual sample introduction of the micro-fluidic chip is surveyed The terminal that sample solution flows in its chip during examination, the terminal is located remotely from each other with the sample introduction end, between the terminal and the sample introduction end Distance between 3 centimetres and 10 centimetres, be sequentially equiped with diverse location in the pipeline working electrode and to electrode with And reference electrode, the working electrode are resisted by conductive electrode and the syphilis specific that embedded being attached on the conductive electrode The gold size sensitive membrane of body is formed, and the construction of the pipeline is in parallel construction, the pipeline in parallel construction by four laterals simultaneously Connection is formed, and the quantity of the working electrode is four, and the installation position of four working electrodes is located at four branched pipes respectively In road, and, the specific antibody in its top layer gold size sensitivity membrane structure of four working electrodes is respectively can be special to Treponema pallidum antigen The opposite sex combine four kinds of syphilis antibody materials, four kinds of antibody materials be respectively syphilis specific antibody TP0684, TP0453, TP0821 and TP0319, its material of the working electrode are that gold material or thermal decomposition conducting polymer material, the work are electric Extremely sheet or thread is presented in pattern, it is characterised in that its material of the substrate is dimethyl silicone polymer material, its table of the substrate Face is the surface of primary form, the surface of the primary form its be intended to refer to and do not pass through any surface chemical modification or any table The surface of the primary form of the material of face chemical modification, the structure of the device also include hinge-type fixture, the hinge-type fixture its Appearance profile is similar to hinge, and the hinge-type fixture is by two hinges being mutually hinged and one through two hinges Fastening screw and one matched with the fastening screw and with the fastening screw connect together be used to fasten manually and pine manually De- nut is formed, and two hinges of the hinge-type fixture are respectively mutually drawn close with its tip and clamp the micro-fluidic chip, the end The tip is positioned in the locations of structures of the neighbouring terminal of the micro-fluidic chip, is attached in a hinge at least in solid Surely miniature ultrasonic transducer units are equiped with, and, higher-order of oscillation electric signal transmission cable, the higher-order of oscillation electric signal transmission cable One end linked together with the miniature ultrasonic transducer units；The hinge-type fixture provides the work(for facilitating the device to disassemble Energy；Its major function of the miniature ultrasonic transducer units be in the test of micro-fluidic chip actual sample introduction, using the sample introduction end and In the distance between the terminal and the miniature ultrasonic transducer units installation position difference and its ultrasonic intensity experienced Difference, the difference between its interfacial tension of sample introduction end described in induced synthesis and the terminal its interfacial tension, the micro-fluidic chip Interfacial tension difference between the both ends can form pressure gap between the both ends of the micro-fluidic chip, and the pressure gap can drive Sample solution is moved to the end flow；Softness simultaneously has its function of the substrate of the dimethyl silicone polymer material of elasticity including with it To the property of the strong absorption of ultrasonic wave, ultrasonic wave is absorbed strongly, and thereby in micro-fluidic chip terminal to the sample introduction The rapid decrement of ultrasonic intensity is realized within limited short distance between end；And Micropump, the Micropump connect with the sample introduction end Connect, the Micropump is built-in Micropump or external Micropump；The function of the Micropump is, with the mechanicalness pumping strength of the Micropump come with Interfacial tension difference its caused driving force between the both ends of ultrasonic wave induction supports mutually, adjustment, mutually mutually It is coupled, the strength that one driving test liquid flows to the terminal direction flowing is pooled in a manner of Collaboration；The cover plate And substrate its thickness is between 1.0 millimeters and 5.0 millimeters；And many silica dioxide granules, many dioxies Silicon carbide particle is filled in the pipeline, and its tube chamber of the pipeline is filled up by many silica dioxide granules, the silica For its particle size range of particle between 10 microns and 200 microns, the silica dioxide granule is silica crystalline particles, dioxy SiClx glass particle or silica composition its inorganic glass particles of shared percentage by weight more than 80%.

A kind of 2. multichannel syphilis diagnosis micro flow control chip device according to claim 1, it is characterised in that the pipe It is capillary channel that road, which includes the lateral,.

A kind of 3. multichannel syphilis diagnosis micro flow control chip device according to claim 1, it is characterised in that the heat It is the conductive material formed by polyimides or polyacrylonitrile after anoxybiotic is heat-treated to decompose conducting polymer.

A kind of 4. multichannel syphilis diagnosis micro flow control chip device according to claim 1, it is characterised in that the work Make electrode width or diameter between 0.1 micron to 2000 microns, and, the length of the working electrode is at 1 micron To between 15000 microns.

A kind of 5. multichannel syphilis diagnosis micro flow control chip device according to claim 1, it is characterised in that the gold The thickness of glue sensitive membrane is between 10 nanometers and 200 nanometers.

6. a kind of multichannel syphilis diagnosis micro flow control chip device according to claim 1, it is characterised in that in structure The cover plate its material be dimethyl silicone polymer material.

7. a kind of multichannel syphilis diagnosis micro flow control chip device according to claim 1, it is characterised in that described micro- Pump is miniature piezoelectric pump, miniature peristaltic pump or miniature air driven pump.

A kind of 8. multichannel syphilis diagnosis micro flow control chip device according to claim 1, it is characterised in that the dioxy Its particle size range of silicon carbide particle is between 100 microns and 200 microns, and its inside diameter ranges of the pipeline are between 500 microns and 1000 Between micron.

A kind of 9. multichannel syphilis diagnosis micro flow control chip device according to claim 1, it is characterised in that the device Structure also include higher-order of oscillation electric signal generator, its other end of the higher-order of oscillation electric signal transmission cable and the higher-order of oscillation Electric signal generator connects.

10. a kind of multichannel syphilis diagnosis micro flow control chip device according to claim 1, it is characterised in that this is miniature Its specified ultrasonic wave transmission power of ultrasonic transducer between 5 milliwatts and 9000 milliwatts, the miniature ultrasonic transducer units its The frequency for the ultrasonic wave operationally launched is between 100KHz and 12MHz.