CN107550768A - A kind of preparation method of marine alga skin care ferment - Google Patents
A kind of preparation method of marine alga skin care ferment Download PDFInfo
- Publication number
- CN107550768A CN107550768A CN201710828916.XA CN201710828916A CN107550768A CN 107550768 A CN107550768 A CN 107550768A CN 201710828916 A CN201710828916 A CN 201710828916A CN 107550768 A CN107550768 A CN 107550768A
- Authority
- CN
- China
- Prior art keywords
- marine alga
- mass ratio
- preparation
- skin care
- conditions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Abstract
The present invention relates to a kind of preparation method of marine alga skin care ferment, by seaweed extracted liquor, emulsifying agent, NMF, nourishing agent, thickener, pearling agent, essence and water be added in homogenizer it is at least homogeneous once, the homogeneous thing of gained is marine alga skin care ferment.The present invention uses new fresh seaweed, and seaweed growth itself has good self-protection function in high salt, cold seawater, can effectively repair skin.
Description
Technical field
The present invention relates to a kind of preparation method of marine alga skin care ferment.
Background technology
Hormone face is also referred to as hormonal acne, belongs to one kind of acne medicamentosa, is because interruption is either prolonged indiscriminate
With hormone ointment or the cosmetics of hormone in secret, cause a kind of severe skin disease caused by the toxic side effect of hormone.It
Common skin sensitivity or allergy are different from, because of its not only serious normal physiological 26S Proteasome Structure and Function for destroying skin, also
Influence is caused on the whole health of patient, to also bringing very big puzzlement in treatment.Symptom of the same name has facial hormone
The call of dependence facial dermatitis, steroid dependent dermatitis.
The many skin care item of in the market are blent to improve whitening effect using chemokine class and formed, and adding has cutin of dispelling
Organic acid, such as conventional salicylic acid, whitening anti-acne effect is with regard to fine, but long-term use can cause to damage to keratoderma
Evil, trigger hormone face.
The content of the invention
The technical problem to be solved by the invention is to provide a kind of preparation method of marine alga skin care ferment, the present invention can be with
Effectively repair skin.
In order to solve the above-mentioned technical problem, the present invention uses following technical scheme：
A kind of preparation method of marine alga skin care ferment, it is characterised in that：
By seaweed extracted liquor, emulsifying agent, NMF, nourishing agent, thickener, pearling agent, essence and water according to mass ratio 10
~40:5~20:5~15:10~20:0.2~1:5~10:0.2~2:5~35 are added in homogenizer, temperature be 50~
80 DEG C, pressure be 2~45MPa under the conditions of at least it is homogeneous once, the homogeneous time is 10~40min, and the homogeneous thing of gained is marine alga
Skin care ferment；
The emulsifying agent is polyglyceryl fatty acid ester, lauric monoglyceride, methyl glycol fatty acid ester, sorbitol anhydride list palm fibre
Glycerin monostearate, stearoyl lactate, diacetyl tartarate monoglyceride, NPE, polyoxyethylene oleic acid ester, tristearin
Sour polyoxyethylene ester, sorbitan fatty acid ester, two polyglycereol, diisooctyl Succinate sodium sulfonate and aliphatic acid polyoxy second
One or any ratio in alkene fat it is two or more；
The NMF is glycerine, fructose, propane diols, sorbierite, polyglutamic acid, niacinamide, polyethylene glycol and xylitol
In one or any ratio it is two or more；
The nourishing agent is honey, sodium salicylate, superoxide dismutase, Alphalin, beta glucan, vitamin e, bad ammonia
One or any ratio in sour sodium and vitamin E it is two or more；
The pearling agent be pearl powder, stearic acid diethylene glycol dilaurate, one kind in ethylene glycol double stearic acid and mica powder or
Person's arbitrary proportion it is two or more；
The thickener is one kind in sodium chloride, sodium carboxymethylcellulose, gelatin, sodium alginate and casein or appointed
Meaning ratio it is two or more；
The seaweed extracted liquor, is followed the steps below：
1) marine alga fragment, is prepared：Marine alga after cleaning is crushed, obtains marine alga fragment；
2) saccharification marine alga, is prepared：By marine alga fragment and water according to mass ratio 5~30:70~95 are fitted into agitator tank,
1~12h is stirred under the conditions of 50~100 DEG C, must be saccharified marine alga；
3) marine alga filtrate and marine alga slag, are prepared：To be saccharified marine alga press filtration, and gained filtrate is marine alga filtrate, residue obtained to be
Marine alga slag；
4) fermentation of seaweed thing, is prepared：By marine alga slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to
Mass ratio 95.5:1:0.5:1:1:1 is added in the fermentation tank to sterilize and mixes, and is adjusted to water content as 50~70% with water, obtains
The mass ratio of mixed culture medium, access candida utili, candida utili and mixed culture medium is 0.2~2:98~
99.8, it is 25~35 DEG C in temperature, under the conditions of oxygen content is 0.1~0.5mg/L, ferments 2~7 days, is 70~150 DEG C in temperature
Under the conditions of sterilize 20~90min, obtain fermentation of seaweed thing；
5) seaweed extracted liquor and marine alga residue, are prepared：The marine alga filtrate that step 3) obtains is added to what step 4) obtained
Stirred and evenly mixed in fermentation of seaweed thing, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, prepared by mixture and thawing method
The mass ratio of corynebacterium ammoniagenes is 95~99:1~5, it is 25~35 DEG C in temperature, under the conditions of oxygen content is 1~5mg/L, fermentation 2
~7 days, gained filtrate was seaweed extracted liquor after press filtration；Filter residue is marine alga residue；
The marine alga is that one kind in Enteromorpha, sea-tangle, sargassum, opotism and kelp or arbitrary proportion are two or more.
Preferably, homogeneous for twice, wherein first time homogeneous pressure is 30MPa in the homogenizer, the homogeneous time is
20min；Second of homogeneous pressure is 4PMa, and the homogeneous time is 20min.
The preparation of thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration
In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for
Strain；
B) thawing method improves the permeability of cell：Thawing method is at least repeated once, and its step is：The expansion that step a) is obtained
It is further cultured for strain greatly to be put into -20 DEG C of refrigerator, freezes 4h, takes out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone
10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, weighs 550 grams of immersions
In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Preferably, the step 1) marine alga is Fresh Laminaria Japonica.
Preferably, the mass ratio 20 of step 2) the marine alga fragment and water:80；Saccharification temperature is 65 DEG C, speed of agitator
For 20~80r/min, 6h is stirred.
Preferably, the step 4) mixed culture medium to water content is 60%, candida utili and mixed culture medium
Mass ratio is 0.5:99.5, it is 30 DEG C in temperature, under the conditions of oxygen content is 0.2mg/L, ferments 6 days, be 120 DEG C of conditions in temperature
Lower sterilizing 20min.
Preferably, the mass ratio of corynebacterium ammoniagenes prepared by step 5) mixture and thawing method is 98:2, it is 28 in temperature
DEG C, under the conditions of oxygen content is 3mg/L, ferment 5 days.
Preferably, the candida utili and corynebacterium ammoniagenes are purchased from the management of Chinese industrial Microbiological Culture Collection
The heart, numbering are respectively CICC 31170 and CICC 10168.
Preferably, the emulsifying agent is polyglyceryl fatty acid ester, lauric monoglyceride and sorbitol anhydride monopalmitate
According to mass ratio 3:2:2 composition；The NMF is glycerine, fructose and polyglutamic acid according to mass ratio 1:2:2 combination
Thing；The nourishing agent is honey, superoxide dismutase and vitamin E according to mass ratio 2:1:1 composition；The pearly-lustre
Agent is pearl powder and stearic acid diethylene glycol dilaurate according to mass ratio 5:1 composition；The thickener is sodium chloride；Marine alga is extracted
Liquid, emulsifying agent, NMF, nourishing agent, thickener, pearling agent, the mass ratio of essence and water are 25:5:10:15:0.5:9:0.5:
35。
Invention has following advantageous effects：
1st, the present invention use new fresh seaweed, seaweed growth in high salt, cold seawater, itself have well self
Defencive function.
2nd, the present invention is fermented under anaerobic using the candida utili of aerobic and anaerobism, can prevent seaweed nutritive into
The loss divided, and alcohol caused by anaerobic fermentation has booster action to marine alga extraction.
3rd, the present invention is fermented under anoxic conditions using aerobic and anaerobism corynebacterium ammoniagenes, produces ATP, and ATP is a kind of
High energy phosphate compound, in cell, it can realize energy storage and exoergic with ADP mutual conversion, so as to ensure that cell items
The energy supply of vital movement, repair damaged skin.The reduzate of corynebacterium ammoniagenes is in alkalescent, can be repaired because making for a long time
The cuticula damaged with organic acid.
4th, the permeability of corynebacterium ammoniagenes cell is improved by thawing method, ATP caused by corynebacterium ammoniagenes can be made more to hold
Extracellular, product of the product effects significantly better than undressed corynebacterium ammoniagenes is easily penetrated into from cell membrane.
Embodiment
The present invention is further illustrated with reference to instantiation.
Embodiment 1
By seaweed extracted liquor, emulsifying agent, NMF, nourishing agent, thickener, pearling agent, essence and water according to mass ratio 25:
5:10:15:0.5:9:0.5:35 are added in homogenizer, and homogeneous under the conditions of being 55 DEG C in temperature is twice, wherein even for the first time
Matter pressure is 25MPa, and the homogeneous time is 25min；Second of homogeneous pressure is 4PMa, and the homogeneous time is 20min, the homogeneous thing of gained
As marine alga skin care ferment.
The preparation technology of seaweed extracted liquor, is followed the steps below：
1) marine alga fragment, is prepared：Fresh Laminaria Japonica after cleaning is crushed, obtains marine alga fragment；
2) saccharification marine alga, is prepared：By marine alga fragment and water according to mass ratio 20:80 are fitted into agitator tank, at 65 DEG C, stir
Under the conditions of mix rotating speed is 45r/min, 6h is stirred, must be saccharified marine alga；
3) marine alga filtrate and marine alga slag, are prepared：Using the filter press of 650 mesh filter clothes to the marine alga press filtration that is saccharified, gained filtrate
For marine alga filtrate, residue obtained is marine alga slag；
4) fermentation of seaweed thing, is prepared：By marine alga slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to
Mass ratio 95.5:1:0.5:1:1:1 is added in the fermentation tank to sterilize and mixes, and is adjusted to water content as 60% with water, must mix
The mass ratio of culture medium, access candida utili, candida utili and mixed culture medium is 0.5:99.5, it is 30 in temperature
DEG C, under the conditions of oxygen content is 0.2mg/L, ferment 6 days, sterilize 20min under the conditions of being 120 DEG C in temperature, obtains fermentation of seaweed thing；
5) seaweed extracted liquor and marine alga residue, are prepared：The marine alga filtrate that step 3) obtains is added to what step 4) obtained
Stirred and evenly mixed in fermentation of seaweed thing, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, prepared by mixture and thawing method
The mass ratio of corynebacterium ammoniagenes is 98:2, it is 28 DEG C in temperature, under the conditions of oxygen content is 3mg/L, ferments 5 days, gained after press filtration
Filtrate is seaweed extracted liquor；Filter residue is marine alga residue；
The preparation of wherein thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration
In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for
Strain；
B) thawing method improves the permeability of cell：In triplicate, its step is thawing method：The expansion that step 1) is obtained is again
Culture strain is put into -20 DEG C of refrigerator, freezes 4h, is taken out, is dissolved, be placed again into -20 DEG C of refrigerator at room temperature, is freezed
4h, take out, dissolve, be placed again into -20 DEG C of refrigerator at room temperature, freeze 4h, take out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone
10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, weighs 550 grams of immersions
In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Embodiment 2
By seaweed extracted liquor, emulsifying agent, NMF, nourishing agent, thickener, pearling agent, essence and water according to mass ratio 25:
5:10:15:0.5:9:0.5:35 are added in homogenizer, and homogeneous under the conditions of being 55 DEG C in temperature is twice, wherein even for the first time
Matter pressure is 25MPa, and the homogeneous time is 25min；Second of homogeneous pressure is 4PMa, and the homogeneous time is 20min, the homogeneous thing of gained
As marine alga skin care ferment.
The preparation technology of seaweed extracted liquor, is followed the steps below：
1) marine alga fragment, is prepared：Fresh Laminaria Japonica after cleaning is dried, dry sea-tangle is crushed using pulverizer, obtained
Marine alga fragment；
2) saccharification marine alga, is prepared：By marine alga fragment and water according to mass ratio 20:80 are fitted into agitator tank, at 65 DEG C, stir
Under the conditions of mix rotating speed is 45r/min, 6h is stirred, must be saccharified marine alga；
3) marine alga filtrate and marine alga slag, are prepared：Using the filter press of 650 mesh filter clothes to the marine alga press filtration that is saccharified, gained filtrate
For marine alga filtrate, residue obtained is marine alga slag；
4) fermentation of seaweed thing, is prepared：By marine alga slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to
Mass ratio 95.5:1:0.5:1:1:1 is added in the fermentation tank to sterilize and mixes, and is adjusted to water content as 60% with water, must mix
The mass ratio of culture medium, access candida utili, candida utili and mixed culture medium is 0.5:99.5, it is 30 in temperature
DEG C, under the conditions of oxygen content is 0.2mg/L, ferment 6 days, sterilize 20min under the conditions of being 120 DEG C in temperature, obtains fermentation of seaweed thing；
5) seaweed extracted liquor and marine alga residue, are prepared：The marine alga filtrate that step 3) obtains is added to what step 4) obtained
Stirred and evenly mixed in fermentation of seaweed thing, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, prepared by mixture and thawing method
The mass ratio of corynebacterium ammoniagenes is 98:2, it is 28 DEG C in temperature, under the conditions of oxygen content is 3mg/L, ferments 5 days, gained after press filtration
Filtrate is seaweed extracted liquor；Filter residue is marine alga residue；
The preparation of wherein thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration
In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for
Strain；
B) thawing method improves the permeability of cell：In triplicate, its step is thawing method：The expansion that step 1) is obtained is again
Culture strain is put into -20 DEG C of refrigerator, freezes 4h, is taken out, is dissolved, be placed again into -20 DEG C of refrigerator at room temperature, is freezed
4h, take out, dissolve, be placed again into -20 DEG C of refrigerator at room temperature, freeze 4h, take out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone
10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, weighs 550 grams of immersions
In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Embodiment 3
By seaweed extracted liquor, emulsifying agent, NMF, nourishing agent, thickener, pearling agent, essence and water according to mass ratio 5:
5:10:15:0.5:9:0.5:55 are added in homogenizer, and homogeneous under the conditions of being 55 DEG C in temperature is twice, wherein even for the first time
Matter pressure is 25MPa, and the homogeneous time is 25min；Second of homogeneous pressure is 4PMa, and the homogeneous time is 20min, the homogeneous thing of gained
As marine alga skin care ferment；The emulsifying agent is polyglyceryl fatty acid ester, lauric monoglyceride and sorbitol anhydride list palmitic acid
Ester is according to mass ratio 3:2:2 composition；The NMF is glycerine, fructose and polyglutamic acid according to mass ratio 1:2:2 group
Compound；The nourishing agent is honey, superoxide dismutase and vitamin E according to mass ratio 2:1:1 composition；The pearly-lustre
Agent is pearl powder and stearic acid diethylene glycol dilaurate according to mass ratio 5:1 composition；The thickener is sodium chloride.
The preparation technology of seaweed extracted liquor, is followed the steps below：
1) marine alga fragment, is prepared：Fresh Laminaria Japonica after cleaning is crushed, obtains marine alga fragment；
2) saccharification marine alga, is prepared：By marine alga fragment and water according to mass ratio 20:80 are fitted into agitator tank, at 65 DEG C, stir
Under the conditions of mix rotating speed is 45r/min, 6h is stirred, must be saccharified marine alga；
3) marine alga filtrate and marine alga slag, are prepared：Using the filter press of 650 mesh filter clothes to the marine alga press filtration that is saccharified, gained filtrate
For marine alga filtrate, residue obtained is marine alga slag；
4) fermentation of seaweed thing, is prepared：By marine alga slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to
Mass ratio 95.5:1:0.5:1:1:1 is added in the fermentation tank to sterilize and mixes, and is adjusted to water content as 60% with water, must mix
The mass ratio of culture medium, access candida utili, candida utili and mixed culture medium is 0.5:99.5, it is 30 in temperature
DEG C, under the conditions of oxygen content is 0.2mg/L, ferment 6 days, sterilize 20min under the conditions of being 120 DEG C in temperature, obtains fermentation of seaweed thing；
5) seaweed extracted liquor and marine alga residue, are prepared：The marine alga filtrate that step 3) obtains is added to what step 4) obtained
Stirred and evenly mixed in fermentation of seaweed thing, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, prepared by mixture and thawing method
The mass ratio of corynebacterium ammoniagenes is 98:2, it is 28 DEG C in temperature, under the conditions of oxygen content is 3mg/L, ferments 5 days, gained after press filtration
Filtrate is seaweed extracted liquor；Filter residue is marine alga residue；
The preparation of wherein thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration
In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for
Strain；
B) thawing method improves the permeability of cell：In triplicate, its step is thawing method：The expansion that step 1) is obtained is again
Culture strain is put into -20 DEG C of refrigerator, freezes 4h, is taken out, is dissolved, be placed again into -20 DEG C of refrigerator at room temperature, is freezed
4h, take out, dissolve, be placed again into -20 DEG C of refrigerator at room temperature, freeze 4h, take out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone
10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, weighs 550 grams of immersions
In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Embodiment 4
By seaweed extracted liquor, emulsifying agent, NMF, nourishing agent, thickener, pearling agent, essence and water according to mass ratio 10:
5:10:15:0.5:9:0.5:50 are added in homogenizer, and homogeneous under the conditions of being 55 DEG C in temperature is twice, wherein even for the first time
Matter pressure is 25MPa, and the homogeneous time is 25min；Second of homogeneous pressure is 4PMa, and the homogeneous time is 20min, the homogeneous thing of gained
As marine alga skin care ferment.The emulsifying agent is polyglyceryl fatty acid ester, lauric monoglyceride and sorbitol anhydride list palmitic acid
Ester is according to mass ratio 3:2:2 composition；The NMF is glycerine, fructose and polyglutamic acid according to mass ratio 1:2:2 group
Compound；The nourishing agent is honey, superoxide dismutase and vitamin E according to mass ratio 2:1:1 composition；The pearly-lustre
Agent is pearl powder and stearic acid diethylene glycol dilaurate according to mass ratio 5:1 composition；The thickener is sodium chloride.
The preparation technology of seaweed extracted liquor, is followed the steps below：
1) marine alga fragment, is prepared：Fresh Laminaria Japonica after cleaning is crushed, obtains marine alga fragment；
2) saccharification marine alga, is prepared：By marine alga fragment and water according to mass ratio 20:80 are fitted into agitator tank, at 65 DEG C, stir
Under the conditions of mix rotating speed is 45r/min, 6h is stirred, must be saccharified marine alga；
3) marine alga filtrate and marine alga slag, are prepared：Using the filter press of 650 mesh filter clothes to the marine alga press filtration that is saccharified, gained filtrate
For marine alga filtrate, residue obtained is marine alga slag；
4) fermentation of seaweed thing, is prepared：By marine alga slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to
Mass ratio 95.5:1:0.5:1:1:1 is added in the fermentation tank to sterilize and mixes, and is adjusted to water content as 60% with water, must mix
The mass ratio of culture medium, access candida utili, candida utili and mixed culture medium is 0.5:99.5, it is 30 in temperature
DEG C, under the conditions of oxygen content is 0.2mg/L, ferment 6 days, sterilize 20min under the conditions of being 120 DEG C in temperature, obtains fermentation of seaweed thing；
5) seaweed extracted liquor and marine alga residue, are prepared：The marine alga filtrate that step 3) obtains is added to what step 4) obtained
Stirred and evenly mixed in fermentation of seaweed thing, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, prepared by mixture and thawing method
The mass ratio of corynebacterium ammoniagenes is 98:2, it is 28 DEG C in temperature, under the conditions of oxygen content is 3mg/L, ferments 5 days, gained after press filtration
Filtrate is seaweed extracted liquor；Filter residue is marine alga residue；
The preparation of wherein thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration
In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for
Strain；
B) thawing method improves the permeability of cell：In triplicate, its step is thawing method：The expansion that step 1) is obtained is again
Culture strain is put into -20 DEG C of refrigerator, freezes 4h, is taken out, is dissolved, be placed again into -20 DEG C of refrigerator at room temperature, is freezed
4h, take out, dissolve, be placed again into -20 DEG C of refrigerator at room temperature, freeze 4h, take out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone
10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, weighs 550 grams of immersions
In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Embodiment 5
By seaweed extracted liquor, emulsifying agent, NMF, nourishing agent, thickener, pearling agent, essence and water according to mass ratio 15:
5:10:15:0.5:9:0.5:45 are added in homogenizer, and homogeneous under the conditions of being 55 DEG C in temperature is twice, wherein even for the first time
Matter pressure is 25MPa, and the homogeneous time is 25min；Second of homogeneous pressure is 4PMa, and the homogeneous time is 20min, the homogeneous thing of gained
As marine alga skin care ferment；The emulsifying agent is polyglyceryl fatty acid ester, lauric monoglyceride and sorbitol anhydride list palmitic acid
Ester is according to mass ratio 3:2:2 composition；The NMF is glycerine, fructose and polyglutamic acid according to mass ratio 1:2:2 group
Compound；The nourishing agent is honey, superoxide dismutase and vitamin E according to mass ratio 2:1:1 composition；The pearly-lustre
Agent is pearl powder and stearic acid diethylene glycol dilaurate according to mass ratio 5:1 composition；The thickener is sodium chloride.
The preparation technology of seaweed extracted liquor, is followed the steps below：
1) marine alga fragment, is prepared：Fresh Laminaria Japonica after cleaning is crushed, obtains marine alga fragment；
2) saccharification marine alga, is prepared：By marine alga fragment and water according to mass ratio 20:80 are fitted into agitator tank, at 65 DEG C, stir
Under the conditions of mix rotating speed is 45r/min, 6h is stirred, must be saccharified marine alga；
3) marine alga filtrate and marine alga slag, are prepared：Using the filter press of 650 mesh filter clothes to the marine alga press filtration that is saccharified, gained filtrate
For marine alga filtrate, residue obtained is marine alga slag；
4) fermentation of seaweed thing, is prepared：By marine alga slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to
Mass ratio 95.5:1:0.5:1:1:1 is added in the fermentation tank to sterilize and mixes, and is adjusted to water content as 60% with water, must mix
The mass ratio of culture medium, access candida utili, candida utili and mixed culture medium is 0.5:99.5, it is 30 in temperature
DEG C, under the conditions of oxygen content is 0.2mg/L, ferment 6 days, sterilize 20min under the conditions of being 120 DEG C in temperature, obtains fermentation of seaweed thing；
5) seaweed extracted liquor and marine alga residue, are prepared：The marine alga filtrate that step 3) obtains is added to what step 4) obtained
Stirred and evenly mixed in fermentation of seaweed thing, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, prepared by mixture and thawing method
The mass ratio of corynebacterium ammoniagenes is 98:2, it is 28 DEG C in temperature, under the conditions of oxygen content is 3mg/L, ferments 5 days, gained after press filtration
Filtrate is seaweed extracted liquor；Filter residue is marine alga residue；
The preparation of wherein thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration
In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for
Strain；
B) thawing method improves the permeability of cell：In triplicate, its step is thawing method：The expansion that step 1) is obtained is again
Culture strain is put into -20 DEG C of refrigerator, freezes 4h, is taken out, is dissolved, be placed again into -20 DEG C of refrigerator at room temperature, is freezed
4h, take out, dissolve, be placed again into -20 DEG C of refrigerator at room temperature, freeze 4h, take out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone
10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, weighs 550 grams of immersions
In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Embodiment 6
By seaweed extracted liquor, emulsifying agent, NMF, nourishing agent, thickener, pearling agent, essence and water according to mass ratio 10:
5:10:15:0.5:9:0.5:50 are added in homogenizer, and homogeneous under the conditions of being 55 DEG C in temperature is twice, wherein even for the first time
Matter pressure is 25MPa, and the homogeneous time is 25min；Second of homogeneous pressure is 4PMa, and the homogeneous time is 20min, the homogeneous thing of gained
As marine alga skin care ferment；The emulsifying agent is polyglyceryl fatty acid ester, lauric monoglyceride and sorbitol anhydride list palmitic acid
Ester is according to mass ratio 3:2:2 composition；The NMF is glycerine, fructose and polyglutamic acid according to mass ratio 1:2:2 group
Compound；The nourishing agent is honey, superoxide dismutase and vitamin E according to mass ratio 2:1:1 composition；The pearly-lustre
Agent is pearl powder and stearic acid diethylene glycol dilaurate according to mass ratio 5:1 composition；The thickener is sodium chloride.
The preparation technology of seaweed extracted liquor, is followed the steps below：
1) marine alga fragment, is prepared：Fresh Laminaria Japonica after cleaning is crushed, obtains marine alga fragment；
2) saccharification marine alga, is prepared：By marine alga fragment and water according to mass ratio 20:80 are fitted into agitator tank, at 65 DEG C, stir
Under the conditions of mix rotating speed is 45r/min, 6h is stirred, must be saccharified marine alga；
3) marine alga filtrate and marine alga slag, are prepared：Using the filter press of 650 mesh filter clothes to the marine alga press filtration that is saccharified, gained filtrate
For marine alga filtrate, residue obtained is marine alga slag；
4) fermentation of seaweed thing, is prepared：By marine alga slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to
Mass ratio 95.5:1:0.5:1:1:1 is added in the fermentation tank to sterilize and mixes, and is adjusted to water content as 60% with water, must mix
The mass ratio of culture medium, access candida utili, candida utili and mixed culture medium is 0.5:99.5, it is 30 in temperature
DEG C, under the conditions of oxygen content is 0.2mg/L, ferment 6 days, sterilize 20min under the conditions of being 120 DEG C in temperature, obtains fermentation of seaweed thing；
5) seaweed extracted liquor and marine alga residue, are prepared：The marine alga filtrate that step 3) obtains is added to what step 4) obtained
Stirred and evenly mixed in fermentation of seaweed thing, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, prepared by mixture and thawing method
The mass ratio of corynebacterium ammoniagenes is 98:2, it is 28 DEG C in temperature, under the conditions of oxygen content is 3mg/L, ferments 5 days, gained after press filtration
Filtrate is seaweed extracted liquor；Filter residue is marine alga residue；
The preparation of wherein thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration
In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for
Strain；
B) thawing method improves the permeability of cell：In triplicate, its step is thawing method：The expansion that step 1) is obtained is again
Culture strain is put into -20 DEG C of refrigerator, freezes 4h, is taken out, is dissolved, be placed again into -20 DEG C of refrigerator at room temperature, is freezed
4h, take out, dissolve, be placed again into -20 DEG C of refrigerator at room temperature, freeze 4h, take out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone
10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, weighs 550 grams of immersions
In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Embodiment 7
By seaweed extracted liquor, emulsifying agent, NMF, nourishing agent, thickener, pearling agent, essence and water according to mass ratio 20:
5:10:15:0.5:9:0.5:30 are added in homogenizer, homogeneous for once, homogeneous pressure is under the conditions of being 55 DEG C in temperature
35MPa, homogeneous time are 25min, and the homogeneous thing of gained is marine alga skin care ferment；The emulsifying agent be lauric monoglyceride and
Sorbitol anhydride monopalmitate is according to mass ratio 3:2 composition；The NMF is glycerine and polyglutamic acid according to mass ratio
1:2 composition；The nourishing agent is honey；The pearling agent is pearl powder；The thickener is sodium chloride.
The preparation technology of seaweed extracted liquor, is followed the steps below：
1) marine alga fragment, is prepared：New fresh sargassum after cleaning is crushed, obtains marine alga fragment；
2) saccharification marine alga, is prepared：By marine alga fragment and water according to mass ratio 10:90 are fitted into agitator tank, at 55 DEG C, stir
Under the conditions of mix rotating speed is 30r/min, 7h is stirred, must be saccharified marine alga；
3) marine alga filtrate and marine alga slag, are prepared：Using the filter press of 800 mesh filter clothes to the marine alga press filtration that is saccharified, gained filtrate
For marine alga filtrate, residue obtained is marine alga slag；
4) fermentation of seaweed thing, is prepared：By marine alga slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to
Mass ratio 95.5:1:0.5:1:1:1 is added in the fermentation tank to sterilize and mixes, and is adjusted to water content as 60% with water, must mix
The mass ratio of culture medium, access candida utili, candida utili and mixed culture medium is 0.7:99.3, it is 30 in temperature
DEG C, under the conditions of oxygen content is 0.4mg/L, ferment 6 days, sterilize 30min under the conditions of being 100 DEG C in temperature, obtains fermentation of seaweed thing；
5) seaweed extracted liquor and marine alga residue, are prepared：The marine alga filtrate that step 3) obtains is added to what step 4) obtained
Stirred and evenly mixed in fermentation of seaweed thing, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, prepared by mixture and thawing method
The mass ratio of corynebacterium ammoniagenes is 97:3, it is 28 DEG C in temperature, under the conditions of oxygen content is 4mg/L, ferments 5 days, gained after press filtration
Filtrate is seaweed extracted liquor；Filter residue is marine alga residue；
The preparation of wherein thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration
In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for
Strain；
B) thawing method improves the permeability of cell：Thawing method is repeated four times, and its step is：The expansion that step 1) is obtained is again
Culture strain is put into -20 DEG C of refrigerator, freezes 4h, is taken out, is dissolved, be placed again into -20 DEG C of refrigerator at room temperature, is freezed
4h, take out, dissolve, be placed again into -20 DEG C of refrigerator at room temperature, freeze 4h, take out, dissolve at room temperature, be placed again into -
In 20 DEG C of refrigerator, 4h is freezed, takes out, dissolves at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone
10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, weighs 550 grams of immersions
In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Beneficial effects of the present invention are further illustrated with reference to experimental data：
Experiment on white mice
1st, acute oral toxicity test：According to《Cosmetics health specification》(2007 editions) are tested to product of the present invention,
Acute oral toxicity test is tested using 10 201g small white mouses, wherein male and female mouse half and half, and disposable dosage of feeding is
5050mg/kg, hair and skin, eyes and mucous membrane through 14 days observation small white mouses, breathing, circulation, autonomic nerve and maincenter god
There are abnormal conditions through system, limb activity and behavior, small white mouse is put to death after off-test and does postmortem, does not find small white mouse device
Official changes.
2nd, acute dermal toxicity is tested：According to《Cosmetics health specification》(2007 editions) are tested to product of the present invention,
Acute dermal toxicity experiment is tested using 10 220g small white mouses, and wherein male and female mouse half and half, disposable test dose are
2180mg/kg, through 14 days observation the trembling of small white mouse, twitch, salivate, suffer from diarrhoea, drowsiness and stupor situation, be shown in Table 1.
Table 1
Small white mouse is put to death after off-test and does postmortem, does not find that small white mouse organ changes.
Application effect is tested
1st, general information
Inventor selects 800 female volunteers to use product of the present invention, year between in September, 2014~2016 year December
Age scope is 22~40 years old.Universal symptom is that pruitus is rubescent, has scurf to come off.This 800 experimenters are divided into 8 immediately
Group, every group of 100 people, test respectively common skin care item (in addition to seaweed extracted liquor is not added, other preparation methods and embodiment 1 one
Cause), contrast 1 (except prepare seaweed extracted liquor when, using corynebacterium ammoniagenes handle without thawing method outside, other preparation methods and
Embodiment 1 is consistent), embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5 and embodiment 6.
2nd, test method
Sooner or later respectively smear once, use 3 months in face.
3rd, judgment criteria and evaluation result
3.1 judgment criteria
It is divided into recovery from illness：Hormone face character disappears, and without recurrence in 1 year；Take a turn for the better：Hormone face symptom mitigation；It is invalid：Skin
It is unchanged；Adverse reaction：Hormone face exacerbation of symptoms after smearing.
3.2 evaluation results statistics such as table 2.
23 groups of Comparison of therapeutic of table
It can be seen that the present invention can be obviously improved hormone face by the data of table 2, skin repaired, by the He of embodiment 1
Embodiment 2 compares it can be seen that seaweed extracted liquor is prepared using Fresh Laminaria Japonica, and the repairing effect of skin is significantly better than and uses it
Dry the effect of thing；By embodiment 1, embodiment 3, embodiment 4, embodiment 5 and embodiment 6 compare it can be seen from embodiment 1 it is each
Component proportion effect is optimal；Embodiment 1 is significantly better than to the repairing effect of skin it can be seen from 1 data of embodiment 1 and contrast
Contrast 1 (except prepare seaweed extracted liquor when, using corynebacterium ammoniagenes handle without thawing method outside, other preparation methods with implementation
Example 1 is consistent).
Claims (9)
- A kind of 1. preparation method of marine alga skin care ferment, it is characterised in that：By seaweed extracted liquor, emulsifying agent, NMF, nourishing agent, thickener, pearling agent, essence and water according to mass ratio 10~40: 5~20:5~15:10~20:0.2~1:5~10:0.2~2:5~35 are added in homogenizer, are 50~80 DEG C in temperature, Pressure is at least homogeneous under the conditions of being 2~45MPa, and once the homogeneous time is 10~40min, and the homogeneous thing of gained is marine alga skin care ferment Element；The emulsifying agent is polyglyceryl fatty acid ester, lauric monoglyceride, methyl glycol fatty acid ester, sorbitol anhydride list palmitic acid Ester, stearoyl lactate, diacetyl tartarate monoglyceride, NPE, polyoxyethylene oleic acid ester, stearic acid gather Oxygen vinyl acetate, sorbitan fatty acid ester, two polyglycereol, diisooctyl Succinate sodium sulfonate and aliphatic acid polyethenoxy fat In one or any ratio it is two or more；The NMF is in glycerine, fructose, propane diols, sorbierite, polyglutamic acid, niacinamide, polyethylene glycol and xylitol One or any ratio it is two or more；The nourishing agent is honey, sodium salicylate, superoxide dismutase, Alphalin, beta glucan, vitamin e, Sodium lysinate It is two or more with the one or any ratio in vitamin E；The pearling agent is pearl powder, stearic acid diethylene glycol dilaurate, one kind in ethylene glycol double stearic acid and mica powder or appointed Meaning ratio it is two or more；The thickener is the one or any ratio in sodium chloride, sodium carboxymethylcellulose, gelatin, sodium alginate and casein Example it is two or more；The seaweed extracted liquor, is followed the steps below：1）, prepare marine alga fragment：Marine alga after cleaning is crushed, obtains marine alga fragment；2）, prepare saccharification marine alga：By marine alga fragment and water according to mass ratio 5~30:70~95 are fitted into agitator tank, 50~ 1~12h is stirred under the conditions of 100 DEG C, must be saccharified marine alga；3）, prepare marine alga filtrate and marine alga slag：To be saccharified marine alga press filtration, and gained filtrate is marine alga filtrate, and residue obtained is marine alga Slag；4）, prepare fermentation of seaweed thing：By marine alga slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to quality Than 95.5:1:0.5:1:1:1 is added in the fermentation tank to sterilize and mixes, and is adjusted to water content as 50~70% with water, must mix training The mass ratio of foster base, access candida utili, candida utili and mixed culture medium is 0.2~2:98~99.8, in temperature Spend for 25~35 DEG C, under the conditions of oxygen content is 0.1~0.5mg/L, ferment 2~7 days, gone out under the conditions of being 70~150 DEG C in temperature 20~90min of bacterium, obtain fermentation of seaweed thing；5）, prepare seaweed extracted liquor and marine alga residue：By step 3）Obtained marine alga filtrate is added to step 4）Obtained marine alga Stirred and evenly mixed in fermentate, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, production ammonia prepared by mixture and thawing method The mass ratio of bar bacterium is 95~99:1~5, it is 25~35 DEG C in temperature, under the conditions of oxygen content is 1~5mg/L, fermentation 2~7 My god, gained filtrate is seaweed extracted liquor after press filtration；Filter residue is marine alga residue；The marine alga is that one kind in Enteromorpha, sea-tangle, sargassum, opotism and kelp or arbitrary proportion are two or more.
- 2. the preparation method of marine alga skin care ferment as claimed in claim 1, it is characterised in that：Homogeneous in the homogenizer is two Secondary, wherein first time homogeneous pressure is 30MPa, and the homogeneous time is 20min；Second of homogeneous pressure is 4PMa, and the homogeneous time is 20min。
- 3. the preparation method of marine alga skin care ferment as claimed in claim 1, it is characterised in that：The system of thawing method corynebacterium ammoniagenes It is standby to follow the steps below：a）The expansion of strain is further cultured for：Using the thalline in exponential phase as seed, liquid is inoculated in 10% inoculum concentration In culture medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for strain；b）Thawing method improves the permeability of cell：Thawing method is at least repeated once, and its step is：By step a）Obtained expansion is again Culture strain is put into -20 DEG C of refrigerator, freezes 4h, is taken out, is dissolved at room temperature；PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone 10.0g, NaCl 5.0g, agar 25.0g；The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, is weighed 550 grams and is soaked in In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
- 4. the preparation method of marine alga skin care ferment as claimed in claim 1, it is characterised in that：Step 1）The marine alga is fresh Sea-tangle.
- 5. the preparation method of marine alga skin care ferment as claimed in claim 1, it is characterised in that：Step 2）The marine alga fragment With the mass ratio 20 of water:80；Saccharification temperature is 65 DEG C, and speed of agitator is 20~80r/min, stirs 6h.
- 6. the preparation method of marine alga skin care ferment as claimed in claim 1, it is characterised in that：Step 4）The mixed culture medium It is 60% to water content, the mass ratio of candida utili and mixed culture medium is 0.5:99.5, it is 30 DEG C in temperature, oxygen content Under the conditions of 0.2mg/L, ferment 6 days, sterilize 20min under the conditions of being 120 DEG C in temperature.
- 7. the preparation method of marine alga skin care ferment as claimed in claim 1, it is characterised in that：Step 5）Mixture and thawing method The mass ratio of the corynebacterium ammoniagenes of preparation is 98:2, it is 28 DEG C in temperature, under the conditions of oxygen content is 3mg/L, ferments 5 days.
- 8. the preparation method of the marine alga skin care ferment as described in any one of claim 1 to 6, it is characterised in that：The production Protein Candida and corynebacterium ammoniagenes are purchased from Chinese industrial Microbiological Culture Collection administrative center, and numbering is respectively CICC 31170 and CICC 10168.
- 9. the preparation method of marine alga skin care ferment as claimed in claim 1, it is characterised in that：The emulsifying agent is polyglycerol ester Fat acid esters, lauric monoglyceride and sorbitol anhydride monopalmitate are according to mass ratio 3:2:2 composition；The NMF is Glycerine, fructose and polyglutamic acid are according to mass ratio 1:2:2 composition；The nourishing agent be honey, superoxide dismutase and Vitamin E is according to mass ratio 2:1:1 composition；The pearling agent is pearl powder and stearic acid diethylene glycol dilaurate according to mass ratio 5:1 composition；The thickener is sodium chloride；Seaweed extracted liquor, emulsifying agent, NMF, nourishing agent, thickener, pearling agent, The mass ratio of essence and water is 25:5:10:15:0.5:9:0.5:35.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710828916.XA CN107550768A (en) | 2017-09-14 | 2017-09-14 | A kind of preparation method of marine alga skin care ferment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710828916.XA CN107550768A (en) | 2017-09-14 | 2017-09-14 | A kind of preparation method of marine alga skin care ferment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107550768A true CN107550768A (en) | 2018-01-09 |
Family
ID=60981059
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710828916.XA Withdrawn CN107550768A (en) | 2017-09-14 | 2017-09-14 | A kind of preparation method of marine alga skin care ferment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107550768A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108186443A (en) * | 2018-02-23 | 2018-06-22 | 广州市骄子日化有限公司 | A kind of shampoo of blackening hair and preparation method thereof |
-
2017
- 2017-09-14 CN CN201710828916.XA patent/CN107550768A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108186443A (en) * | 2018-02-23 | 2018-06-22 | 广州市骄子日化有限公司 | A kind of shampoo of blackening hair and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107362074A (en) | A kind of preparation method of algae hair conditioner | |
| CN105053593A (en) | Traditional Chinese medicine residues prepared poultry feed additive and preparation method thereof | |
| CN105919110B (en) | Black tea composite enzyme and preparation method thereof | |
| CN108823261A (en) | A kind of Ultra-low molecular weight Dendrobium officinale polysaccharide and its preparation and application | |
| KR20070097722A (en) | Fermented rice bran composition | |
| CN105255767B (en) | A kind of complex microorganism preparations and fowl fermenting bed padding | |
| CN108186422A (en) | Fermentation Condition of Monascus spp product extract, preparation method and its usage | |
| CN103263448A (en) | Fermentation bacteria used for fermentation pretreatment to improve extraction of Ginkgo biloba L. leaf flavone and application | |
| CN103783321B (en) | Shelled Turtle Trionyx Sinensis probiotic feed additive and preparation method | |
| CN111228130A (en) | Composition with skin cell energy stimulating and skin barrier repairing effects and preparation method and application thereof | |
| CN109939064A (en) | The Essence of the metabolite containing probiotics fermention and its application in cosmetics | |
| CN106946603A (en) | A kind of oil tree peony fertilizer special for organic and preparation method thereof | |
| CN111904878B (en) | Preparation method and application of liposome containing rose fermentation liquor | |
| CN107898677A (en) | A kind of preparation method of plant skin care ferment | |
| CN107488616A (en) | Strain, probiotics facial mask, the preparation method of health preserving fungi bacterium tea and edible fungi tea | |
| CN107550768A (en) | A kind of preparation method of marine alga skin care ferment | |
| KR20200136964A (en) | Method for producing a composition for treating hair loss | |
| CN103725738B (en) | The method preparing collagen polypeptide with Carnis Pseudosciaenae leftover bits and pieces | |
| CN107998002A (en) | A kind of preparation method of plant enzyme facial mask | |
| CN104644538B (en) | Radix scutellariae lactobacillus fermentation, the cosmetics containing fermentate and preparation method and purposes | |
| CN111904909A (en) | Dandruff-removing scalp essence containing rose fermentation liquor and preparation method thereof | |
| CN106726654A (en) | A kind of xylitol moisture-keeping composition and its application in cosmetics | |
| CN107550769A (en) | A kind of preparation method of marine alga ferment facial mask | |
| CN115282093B (en) | Anti-aging wrinkle-removing composition and repairing eye cream containing same | |
| CN101181314A (en) | Method for preparing folium ginkgo extract from Hericium erinaceus and usage |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180109 |