CN107543921A - A kind of method and detection kit assessed dog and breed situation - Google Patents
A kind of method and detection kit assessed dog and breed situation Download PDFInfo
- Publication number
- CN107543921A CN107543921A CN201610475780.4A CN201610475780A CN107543921A CN 107543921 A CN107543921 A CN 107543921A CN 201610475780 A CN201610475780 A CN 201610475780A CN 107543921 A CN107543921 A CN 107543921A
- Authority
- CN
- China
- Prior art keywords
- dog
- progesterone
- detection
- breed
- bitch
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Assess dog the invention discloses a kind of and breed the method and detection kit of situation, it is characterised in that the structure of kit is：Sample pad (2) is pasted in plastic bottom board (1) one end, closely crimping contains the pad (3) for marking anti-dog progesterone specific antibody, pad for one end of sample pad（3）One end closely crimps nitrocellulose NC films (4), the detection line T1 (5) being separated from each other, detection line T2 (6) and nature controlling line C (7) are coated with NC films, the other end connection adsorptive pads (8) of NC films form test paper, and test paper loads plastics and got stuck interior formation test card.By in bitch serum progesterone and the change, corresponding breeding, pregnancy, childbirth for assessing bitch etc. of numerical value content of interstitialcellstimulating hormone (ICSH) breed situation.Have the advantages that it is easy to operate, quick, be adapted to Site Detection and economical and practical.
Description
Technical field
The present invention is to be related to biologic applications technical field, more particularly to one kind using fluorescent microsphere lateral chromatography technology as base
A kind of assessment dog prepared by the competition law of plinth breeds the method and detection kit of situation, the gestation auxiliary especially suitable for canine
Diagnosis.
Background technology
In recent years, dog technology of artificial insemination gradually occupies consequence in dog auxiliary procreation technology, judges that dog is arranged
Ovum opportunity and appropriate fertilization opportunity are important all the more.Vaginal fluid smear, serum sex hormone level measure, ultrasonic examination
The means of ovulation prediction are normally used as, however, people judge that dog ovulates opportunity there is presently no standard set is made
Operation scheme.
The breeding cycle of various mammals is substantially similar.The breeding cycle of bitch compared with other mammals, has
Its particularity.Dog is seasonal single or double secondary heat animals, typically in annual spring and estrus in fall.The oestrous cycle bag of dog
Include proestrum, oestrus, heat later stage and dioestrus.Because the reproductive physiology of dog is different from other mammals, it is ovulated
Opportunity, become pregnant, the physiological change process of embryonic development and gestation it is important for undergoing an unusual development for dog auxiliary procreation technology,
The wherein most important point, it is exactly to grasp accurate ovulation, breeding and timing of delivery.
Progesterone (Progesterone, P4) is a kind of steroid hormone of corpus luteum and placenta secretion in canine body, bitch one
Individual pregnant week phase progesterone value performance is risen day by day by low, declines after 15 days phases of peaking, low-level 0ng/ml, dog were dropped to by 19 days
Progesterone continues to higher level after becoming pregnant, and serum progesterone reaches 30ng/ml within 26~30 days.The present invention captures progesterone in serum
Periodically variable rule, using fluorescent quantitation immunochromatographic method principle, using progesterone as detection object, sentenced with progesterone level
Whether disconnected bitch is pregnant, assesses delivery time, and exclude " false pregnancy " phenomenon.
The cyclostage of dog is to be listened regulation and control by the gonad axis of one hypophysis of hypothalamus one.The gonadotropin releasing hormone of hypothalamus
Hormonal stimulation anterior pituitary, FSH (FSH) and interstitialcellstimulating hormone (ICSH) (LH) are produced, in FSH and LH collective effect
Under, estrogen concentrations gradually rise up to peak, so as to stimulate LH pulsed releases.When in blood LH concentration reach peak value after can touch
Send out ovary ovulation.Now, the ovarian follicle after ovulation is gradually degenerated, and is formed corpus luteum and is entered luteal phase.It is yellow with the gradual maturation of corpus luteum
Body release progesterone (P4).As bitch enters the heat stage, the concentration of Concentration of Progesterone gradually rises in blood, straight in bitch pregnancy
To before childbirth, progesterone is constantly in high value state, and the amount of progesterone drastically declines in term, bitch serum.
The rise of diestrum end LH concentration stimulates the synthesis of androgen precurosor, is utilized by FSH dependence aromatizing enzymes.LH
Concentration raises 7 days or after the longer time, estrogen synthetic quantity peaks, after the semi-autonomous development of about about 2-5 days, ovarian follicle
Stimulate the ability of estradiol production to be gradually reduced, and start a large amount of secretion progesterone, LH pulse peaks and enter heat before provoked ovulation
Phase, and along with the completion of follicular development final stage.With LH pulse peaks, ovarian follicle secretion and paracrine mechanism and other lactations
Animal is similar.If bitch is not pregnant, duration luteal phase is about 55-75 days.Pregnancy dog then enters the gestational period, about 60 days.
Because serum Lh peak value only maintains 24h, it is therefore necessary to carry out strict prison side to bitch serum sample.From proestrum the 4th or five
It starts, or when vaginal cytology shows 50% or so cuticulated epithelium, blood sampling of same time daily carries out LH detections.Blood sampling
Need within first day to determine Concentration of Progesterone baseline simultaneously.If LH is detected again after occurring in oestrus, then probably wrong
LH peak value periods are spent, can not differentiate whether LH concentration is in the decline stage.So need to progesterone in bitch serum and LH connection
Conjunction detection, the present invention show that bitch breeds period progesterone and LH variable pole by the analysis of the gestation activity to a variety of canines
With regularity.
Canine auxiliary procreation technology is the important technique measure for improving dog reproductive efficiency and canine farming productivity effect, and bitch is matched somebody with somebody
After kind, cyesiognosis can be carried out as soon as possible, it is nonpregnant for reducing, distinguish that false pregnancy, raising conception rate, assessment Parturient Environment all have
Highly important meaning.It is determined that the bitch of gestation should strengthen the management of each side, the health of bitch is kept.If it is determined that gestation should not
Find the cause in time, to be improved or to treat in time.Canine cyesiognosis technology is that solve and overcome numerous in bitch at present
Grow " false pregnancy " problem existing for aspect.So as to improve the economic benefit bred quality, dog master is supported with raising of bitch.
Existing dog Technology on Early-Pregnancy Diagnosis has a variety of methods, such as：Observation, enzymoimmunoassay, ultrasonic diagnosis
Method etc., wherein observation are the most frequently used diagnostic methods, the change of observation observation bitch behavior figure and vulva, are tried with public dog
Feelings, injection female hormone etc., it is not accurate enough although method is simple and easy, it can be caused in dog body using female hormone too early
Parasecretion causes long-term out of heat；Examination per rectum method is only applicable to the larger bitch of build, has certain limitation, using not
It is more；Hormone determination mainly uses radioimmunology or ELISA, and accuracy is higher, but cumbersome, costly, typically
For scientific research；The not only once input of ultrasonic diagnosis is too big, and also needs to technical professional.Document report
30 days diagnostic accordance rates of pregnant dog are 85%, and 40 days diagnostic accordance rates of pregnant dog can be only achieved 100%, want to improve in 19~30 days
Pregnant dog diagnosis rate, there must be higher and accurate detection technique.
Fluorescent quantitation immunochromatography technique is as follows as new diagnostic techniques, general principle：Marked using fluorescent microsphere
One or more antigens or antibody, are coated with corresponding pairing antigen or antibody on the nitrocellulose filter of reagent, work as during detection
When containing corresponding specific antibody or antigen in sample, in fluorescent microsphere marking particle and sample part be combined to be formed it is anti-
Antigen-antibody complex, then chromatographed on nitrocellulose filter, then be coated antigen or antibody capture, formed macroscopic
T lines are detected, under certain conditions, the concentration in the power and sample of T lines is proportionate or negative correlation, so as to realize detection.
There is easy to operate, rapid reaction, sensitiveness height, high specificity, be adapted to Site Detection and economical and practical.
The content of the invention
Assess dog it is an object of the invention to provide a kind of and breed the method and detection kit of situation, have directiveness it is strong,
Easy to operate, rapid reaction, sensitiveness height, high specificity, be adapted to Site Detection and it is economical and practical the advantages that, utilize fluorescent quantitation
Immunochromatographic method principle, using serum progesterone and interstitialcellstimulating hormone (ICSH) as detection object, with progesterone and interstitialcellstimulating hormone (ICSH) water
It is flat to judge that bitch breeds situation.
In order to solve the above technical problems, a kind of method and detection kit assessed dog and breed situation of the present invention, its feature
It is, the structure of kit is：Sample pad (2) is pasted in plastic bottom board (1) one end, closely crimping contains mark for one end of sample pad
Remember the pad (3) of anti-dog progesterone specific antibody, pad (3) one end closely crimps nitrocellulose NC films (4), on NC films
It is coated with the detection line T1 (5) being separated from each other, detection line T2 (6) and nature controlling line C (7), the other end connection adsorptive pads (8) of NC films
Test paper is formed, test paper loads plastics and got stuck interior formation test card.
The fluorescent microsphere of the labelled antibody of the kit can be a kind of metal colloid particles, a kind of latex particle, wrap
Include but be not limited only to magnetic-particle, dye granule.
The fluorescent microsphere labelled antibody of the test card is dog progesterone (P4) specific antibody and dog interstitialcellstimulating hormone (ICSH)
(LH) specific antibody, combined with dog progesterone and interstitialcellstimulating hormone (ICSH) antigentic specificity, detection line T1 coating dog progesterone is manually exempted from
Epidemic focus, detection line T2 coating dog interstitialcellstimulating hormone (ICSH) artificial immunogens, nature controlling line C coating sheep anti-mouse igg antibodies.
Methods described by bitch serum progesterone and interstitialcellstimulating hormone (ICSH) numerical value content change, it is corresponding to comment
Estimate breeding, pregnancy, childbirth of bitch etc. and breed situation.
Described bitch breeds feature and meets Y=k1 (P4)+k2 (LH) rule, by live dog progesterone and estrogen inspection
Measured value, bitch gestation present situation is assessed, wherein k1 values are that 0.874, k2 values are 1.32.
The test card is immediately inserted into matching used fluorescent quantitative detector device, at 15 minutes after adding mouth sample-adding
Afterwards, dog progesterone and interstitialcellstimulating hormone (ICSH) content in sample are accurately shown.
The beneficial effects of the invention are as follows：Assessment dog breeds the method for situation and detection kit uses and lateral layer is completely newly immunized
The technical scheme of analysis, have it is simple to operate, detection time is short, result is clear, without complex operations skill, high sensitivity, without dirt
The advantages that contaminating and be easy to carry.Can be in plant, pet clinic's spot sampling, scene goes out result, convenient and practical.Determined using fluorescence
Immunity principle is measured, using serum progesterone as detection object, situation of being bred to bitch is carried out effectively assessing serum progesterone and promoted yellow
Body generation element judges that bitch breeds situation, so as to substantially reduce as detection object so that progesterone and interstitialcellstimulating hormone (ICSH) are horizontal
The generation of " false pregnancy ", improve diagnosis efficiency and economic benefit.
Brief description of the drawings：
Described above is the general introduction to technical solution of the present invention, for the present invention is explained further, below in conjunction with accompanying drawing and reality
Applying mode, the present invention is described in further detail.
A kind of detection reagent arrangement signal assessed dog and breed the method and detection kit of situation of Fig. 1 present invention
Figure.
Reference numeral explanation：1：Plastic bottom board；2：Sample pad；3：Pad；4：Nitrocellulose NC films；5：Detection line T；
6：Nature controlling line C；7：Adsorptive pads.
A kind of detection reagent whole body structure signal assessed dog and breed the method and detection kit of situation of Fig. 2 present invention
Figure.
Embodiment
Embodiment：A kind of detection kit preparation and detection assessed dog and breed situation
1 main material
1.1 clinical sample：Obtained by company in related pet clinic, totally 50 parts of bitch serum.
1.2 dog progesterone labelled antibodies, dog interstitialcellstimulating hormone (ICSH) labelled antibody：U.S.'s Fitzgerald Products；Dog is pregnant
Ketone standard items：Academy of agricultural sciences；Sheep anti-mouse igg antibody：ICL International Computer Limited of U.S. product；Fluorescent microsphere：Sigma Products；Cellulose nitrate
Plain (NC) film：SARTORIUS (Germany), CN140；Sartorius AG's product；Bovine serum albumin(BSA) (BSA), hydrolyze junket
Albumen：Sigma products.Other common agents are AR.
2.1 dog progesterone labelled antibodies are coupled fluorescent microsphere：Take 1mg, 0.5mg, 0.1mg (10mg/ml) fluorescent microsphere solution
It is respectively placed in centrifuge tube, 500 μ l MES buffer solutions is added after ultrasonic 1.5min.5 μ l activator solutions are taken, it is rapid respectively to be added dropwise
Into microballoon latex solution, it is fixed in vortex oscillator, room temperature concussion 30min.Activation terminates, and takes latex solution to centrifuge, and abandons
Supernatant is removed, is redissolved with 100 μ l PB buffer solutions.Labelled antibody 0.025mg is taken to be added in 400 μ l PB buffer solutions, refrigeration is protected
Deposit.The latex of redissolution is added in antibody dilute solution, is vortexed and mixes 1.5min, ultrasonic 1.5min, in room temperature concussion reaction
1h.Reaction terminates, ultrasonic 1min, centrifugation abandoning supernatant, 500 μ l confining liquids of addition, and concussion reaction 1h (rear eccentric cleaning, respectively
Confining liquid is added to clean, supernatant is removed in centrifugation.It is each to add 500 μ l re-suspension liquids, it is resuspended as 1mg/ml, it is stored refrigerated, it is standby.
2.2 dog interstitialcellstimulating hormone (ICSH) labelled antibodies are coupled fluorescent microsphere：Take 1mg, 0.5mg, 0.1mg (10mg/ml) fluorescence
Microspheres solution is respectively placed in centrifuge tube, and 500 μ l MES buffer solutions are added after ultrasonic 1.5min.5 μ l activator solutions are taken, respectively
It is added drop-wise in microballoon latex solution rapidly, is fixed in vortex oscillator, room temperature concussion 30min.Activation terminates, and takes latex solution
Centrifugation, abandoning supernatant, redissolved with 100 μ l PB buffer solutions.Labelled antibody 0.025mg is taken to be added in 400 μ l PB buffer solutions,
It is stored refrigerated.The latex of redissolution is added in antibody dilute solution, is vortexed and mixes 1.5min, ultrasonic 1.5min, is shaken in room temperature
Swing reaction 1h.Reaction terminates, ultrasonic 1min, centrifuges abandoning supernatant, adds 500 μ l confining liquids, (rear centrifugation is clear by concussion reaction 1h
Wash, respectively plus supernatant is removed in confining liquid cleaning, centrifugation.It is each to add 500 μ l re-suspension liquids, it is resuspended as 1mg/ml, it is stored refrigerated, it is standby.
2.3 spray pad processing：Dog progesterone antibody fluorescence microballoon presses 1 with dog interstitialcellstimulating hormone (ICSH) antibody fluorescence microballoon:1 mixes,
Film instrument, which is drawn, with metal spraying is sprayed on pad, 1h, 37 DEG C of drying process pads.
The line of 2.3NC films is handled：With 0.01M pH7.4PBS by dog progesterone artificial immunogen and dog interstitialcellstimulating hormone (ICSH) people
Work immunizing antigen is diluted to 0.5mg/ml, 1mg/ml, 2mg/ml, and sheep anti-mouse igg is diluted to 1mg/ml, 2mg/ml respectively, then
Film instrument is drawn with metal spraying on NC films to be rule respectively coating by 1 μ l/cm, by NC films in 21-25 DEG C of temperature, phase after the completion of coating
2-5 hours are dried to drying room of the humidity in ＜ 30%.
2.4 test cards assemble：In hothouse, 21-25 DEG C of temperature, humidity is less than 30%, takes plastic bottom board, will be coated with
NC films be placed on plastic bottom board middle part paste, colloidal gold pad is cut into suitable width, NC film T lines side overlap tie
Pad is closed, take pad 1/5 is pasted, and pastes sample pad in pad, and take pad 1/5 is pasted；Overlapped in NC film C lines side
Sample suction pad, take sample suction pad 1/10 are pasted；The plastic plate posted is finally cut into the wide test strips of 3-5mm with cutter, refilled
Enter in plastics get stuck, form reagent test strip card.
2.5 test paper technological parameters are debugged：By concentration, isolabeling, coated reagent are not combined pairing, prepare sample, profit
Reagent is tested with CTNI standard items, finds best of breed.
2.6 detection method：1) liquid is detected to prepare：Whole blood 1-2ml, 1500r/min are gathered, centrifugation, 50 μ are drawn with pipettor
L serum is added in a pipe sample diluting liquid, after fully mixing, as detection liquid.(if sample can not be tested immediately, should refrigerate
Preserve, more than 24h, answer freezen protective).
2) test card not broken a seal and detection sample are recovered to room temperature；3) it is test card is horizontal positioned, to test paper hole clipping
It is slowly added dropwise 120 μ L altogether and detects liquid；4) test card is inserted into rapidly the detection mouth of supporting fluorescent quantitative detector device
In, into test interface, click on " standard testing ", you can read testing result.
2.7 pattern detections are analyzed：After the completion of prepared by reagent, all clinical samples are detected by detection method, and point
Analyse testing result.
3 results
3.1 test paper parameters determine：According to the testing result of sample, it is determined that progesterone antibody optimum mark amount is 0.5mg, is promoted
Lutropin antibody optimum mark amount is 0.1mg；Optimal collaurum working solution is 20mM phosphate buffers, pH8.0, is contained
2%BSA, 10% sucrose, 0.01M Tris, 5% trehalose；Optimal coating V antigen concentrations are 1.0mg/ml.Testing result is most
The good judgement time is 15min.But above parameter may need suitably to adjust when preparing different batches of product.
3.2 pattern detection：To 50 parts of clinical definite value pattern detections, the coincidence rate of section result of determination and sample value is
96.4%, consistency coefficient>0.97.Testing result shows that the Test paper card of preparation is functional, is suitable for clinical detection,
Exist at present in bitch proliferation so as to solve the problems, such as and overcome.So as to which that improves bitch breeds quality, to improve foster dog
Main benefit.
Patent of the present invention discloses a kind of method and detection kit assessed dog and breed situation, it is characterised in that reagent
The structure of box is：Sample pad (2) is pasted in plastic bottom board (1) one end, closely crimping is pregnant containing the anti-dog of mark for one end of sample pad
The pad (3) of ketone specific antibody, pad (3) one end closely crimp nitrocellulose NC films (4), phase are coated with NC films
Detection line T1 (5), detection line T2 (6) and the nature controlling line C (7) mutually separated, the other end connection adsorptive pads (8) of NC films form examination
Paper, test paper load plastics and got stuck interior formation test card.By in bitch serum progesterone and interstitialcellstimulating hormone (ICSH) numerical value
Situation is bred in the change of content, the corresponding breeding, pregnancy, childbirth for assessing bitch etc..With easy to operate, quick, suitable scene
The advantages that detecting and be economical and practical.
Claims (6)
- Assess dog 1. a kind of and breed the method and detection kit of situation, it is characterised in that the structure of kit is：In plastic bottom Sample pad (2) is pasted in plate (1) one end, and closely crimping contains the pad for marking anti-dog progesterone specific antibody for one end of sample pad (3), pad (3) one end closely crimps nitrocellulose NC films (4), be coated with NC films be separated from each other detection line T1 (5), Detection line T2 (6) and nature controlling line C (7), the other end connection adsorptive pads (8) of NC films form test paper, and test paper loading plastics get stuck interior Form test card.
- 2. a kind of method and detection kit assessed dog and breed situation according to claim 1, it is characterised in that described The fluorescent microsphere of the labelled antibody of kit can be a kind of metal colloid particles, a kind of latex particle, include but are not limited to Magnetic-particle, dye granule.
- A kind of 3. method and detection kit assessed dog and breed situation according to claim 1, it is characterised in that test paper The fluorescent microsphere labelled antibody of card is dog progesterone (P4) specific antibody and dog interstitialcellstimulating hormone (ICSH) (LH) specific antibody, with dog Progesterone and interstitialcellstimulating hormone (ICSH) antigentic specificity combine, detection line T1 coating dog progesterone artificial immunogen, detection line T2 coating dogs Interstitialcellstimulating hormone (ICSH) artificial immunogen, nature controlling line C coating sheep anti-mouse igg antibodies.
- 4. a kind of method and detection kit assessed dog and breed situation according to claim 1, it is characterised in that described Method by bitch serum progesterone and interstitialcellstimulating hormone (ICSH) numerical value content change, it is corresponding to assess matching somebody with somebody for bitch Situation is bred in kind, pregnancy, childbirth etc..
- 5. a kind of method and detection kit assessed dog and breed situation according to claim 4, it is characterised in that described Bitch breed feature and meet Y=k1 (P4)+k2 (LH) rule, by the dog progesterone of acquisition and the detected value of estrogen, to mother Dog gestation present situation is assessed, and wherein k1 values are that 0.874, k2 values are 1.32.
- 6. a kind of method and detection kit assessed dog and breed situation according to claim 1, it is characterised in that described Test card is immediately inserted into matching used fluorescent quantitative detector device, over the course of 15 mins, accurately shows sample after adding mouth sample-adding Dog progesterone and interstitialcellstimulating hormone (ICSH) content in this.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610475780.4A CN107543921A (en) | 2016-06-24 | 2016-06-24 | A kind of method and detection kit assessed dog and breed situation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610475780.4A CN107543921A (en) | 2016-06-24 | 2016-06-24 | A kind of method and detection kit assessed dog and breed situation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107543921A true CN107543921A (en) | 2018-01-05 |
Family
ID=60961231
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610475780.4A Pending CN107543921A (en) | 2016-06-24 | 2016-06-24 | A kind of method and detection kit assessed dog and breed situation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107543921A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112578127A (en) * | 2020-11-16 | 2021-03-30 | 爱若维生物科技(苏州)有限公司 | Canine progesterone immunochromatography test strip, preparation method and detection method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201035012Y (en) * | 2007-02-14 | 2008-03-12 | 万华普曼生物工程有限公司 | endocrine hormone four-in-one combined checker board |
| CN101995470A (en) * | 2009-08-31 | 2011-03-30 | 万积成 | Colloid gold immunochromatographic assay diagnosis method for early gestation of sows and detector |
| CN104569433A (en) * | 2014-05-22 | 2015-04-29 | 江苏金标世纪生物科技有限公司 | Myocardial infarction rapid detection kit and preparation method thereof |
-
2016
- 2016-06-24 CN CN201610475780.4A patent/CN107543921A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201035012Y (en) * | 2007-02-14 | 2008-03-12 | 万华普曼生物工程有限公司 | endocrine hormone four-in-one combined checker board |
| CN101995470A (en) * | 2009-08-31 | 2011-03-30 | 万积成 | Colloid gold immunochromatographic assay diagnosis method for early gestation of sows and detector |
| CN104569433A (en) * | 2014-05-22 | 2015-04-29 | 江苏金标世纪生物科技有限公司 | Myocardial infarction rapid detection kit and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| M. V. ROOT KUSTRITZ ET AL.: "Use of Commercial Luteinizing Hormone and Progesterone Assay Kits in Canine Breeding Management", 《INTERNATIONAL VETERINARY INFORMATION SERVICE (WWW.IVIS.ORG)》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112578127A (en) * | 2020-11-16 | 2021-03-30 | 爱若维生物科技(苏州)有限公司 | Canine progesterone immunochromatography test strip, preparation method and detection method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Snijders et al. | Genetic merit for milk production and reproductive success in dairy cows | |
| Lopez et al. | Relationship between level of milk production and estrous behavior of lactating dairy cows | |
| Sasser et al. | Detection of early pregnancy in domestic ruminants | |
| Heap et al. | Oestrone sulphate in milk as an indicator of a viable conceptus in cows | |
| JP2009512871A (en) | Monitoring and management of the ovulation cycle | |
| EP2465469A1 (en) | Device for predicting the optimum insemination time for an animal | |
| CN102297966A (en) | Progesterone semi-quantitative determining colloidal gold test paper and preparation method thereof | |
| Steinhoff et al. | Thyroid hormone profiles and TSH evaluation during early pregnancy and the transition period in dairy cows | |
| Kajaysri et al. | Assessment of pregnancy status of Asian elephants (Elephas maximus) by measurement of progestagen and glucocorticoid and their metabolite concentrations in serum and feces, using enzyme immunoassay (EIA) | |
| Lockwood et al. | Relationships among temperament, acute and chronic cortisol and testosterone concentrations, and breeding soundness during performance testing of Angus bulls | |
| Cabrera et al. | Replacing the first gonadotropin-releasing hormone treatment in an Ovsynch protocol with human chorionic gonadotropin decreased pregnancies per artificial insemination in lactating dairy cows | |
| CN105572394A (en) | Preparation method of urine HCG quantitative detection test paper strip | |
| Tucker et al. | Serum prolactin and growth hormone in Holstein bulls | |
| AU659612B2 (en) | Hormone detection methods | |
| CN107543921A (en) | A kind of method and detection kit assessed dog and breed situation | |
| Panzani et al. | Corpus luteum vascularization and progesterone production in autumn and winter cycles of the mare: Relationship between ultrasonographic characteristics of corpora lutea and plasma progesterone concentration in the last cycles before anestrus | |
| CN103163305A (en) | Method for quickly judging woman pregnancy conditions | |
| Fleming et al. | Serum progesterone determination as an aid for pregnancy diagnosis in goats bred out of season | |
| Lueders et al. | Prolonged luteal lifespan and pseudopregnancy in Asian elephants (Elephas maximus) | |
| Sanders et al. | The development of a simple fecal immunoreactive progestin assay to monitor reproductive function in swine. | |
| Al-Qarawi et al. | Intratesticular morphometric, cellular and endocrine changes around the pubertal period in dromedary camels | |
| Nepal et al. | Pregnancy diagnosis with progesterone ELISA kit in farm animals, its accuracy and application | |
| CN103604934B (en) | Domestic animal chorionic-gonadotropin hormone method for quick and quickly detection test card | |
| Schneyer et al. | Radioimmunoassay of serum follicle-stimulating hormone and luteinizing hormone in the bottlenosed dolphin | |
| JP2002328127A (en) | Kit for diagnosing noncyesis, and method of diagnosing noncyesis in animal using the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180105 |