CN107537592A - Additional liquid sample driving is with the easily-disassembled AIDS diagnosis device of building blocks of function - Google Patents

Abstract

The present invention relates to a kind of easily-disassembled AIDS diagnosis device of additional liquid sample driving building blocks of function, belong to analysis testing field.With dimethyl silicone polymer i.e. PDMS that is cheap and easily processing to make AIDS diagnosis with the substrate of micro-fluidic chip be a technical goal.This case main points are that substrate material is the PDMS for having ecosystem surface, and doped micro-scale titanium dioxide particle is filled with its pipeline of chip；The titanium dioxide granule is the titanium dioxide granule that its surface super hydrophilic property can be induced via advance radiation of visible light；The set in its intergranular super hydrophilic gap road of the titanium dioxide granule filler, it is compensatory, surmounted originally strong hydrophobic pipeline, thus reach constant and super hydrophilic modified effect；Meanwhile the difference of interfacial tension is induced at its head and the tail both ends of the chip with the ultrasonic wave of micropower, thereby guide sample liquid stream to be flowed along its pipeline of chip to the terminal direction.

Description

Additional liquid sample driving is with the easily-disassembled AIDS diagnosis device of building blocks of function

Technical field

The present invention relates to a kind of easily-disassembled AIDS diagnosis device of additional liquid sample driving building blocks of function, the dress Put is to belong to analysis testing field based on antigen/antibody specific reaction come the special purpose device of diagnosis of aids.

Background technology

The micro-fluidic AIDS diagnosis technical background of associated multi-channel, it is special to may refer to the grade inventions of CN 200910151219.0 Sharp application case.

Only for the microflow control technique overall general picture of itself, famous micro-fluidic expert Mr. Lin Ping Cheng may refer to not The monograph " diagram Microfluid based Lab on a chip " gone out before long, the monograph is published via Science Press, and the monograph is to micro-fluidic Past of technology, now, and, vision of the future etc. etc., suffer from detail, be deep into long discussion of detail.

So, the Important Problems of this case that to have a talk below concern.

The basic framework of micro-fluidic chip, including it is etched with the substrate of conduit and the cover plate to fit together therewith, institute State the fluid course on substrate, before upper cover plate is assembled, it is apparent on see to be exactly some conduits, wait until lid is covered thereon After piece, just really closure forms the fluid course, and the conduit inner surface of the conduit is together with the part that surround the conduit Cover plate forms described fluid course together；So, it is clear that assemble the fluid course after completing, its inner surface face Long-pending major part is the inner surface area of that conduit, and in other words, the state or property of the conduit inner surface are substantially determined The integrality or property of the fluid course are determined；Therefore say, the inner surface state or interior of this conduit of the structure on substrate Surface nature is key factor；In principle, any material that can be kept or keep its solid forms substantially, can be used To make substrate and cover plate, such as, the material that can act as substrate and cover plate can be monocrystalline silicon piece, quartz plate, sheet glass, height Polymers such as dimethyl silicone polymer, polymethyl methacrylate, makrolon etc.；Certainly, the selection of substrate and the choosing of cover plate Material be able to can also be differed with identical；From the point of view of material consumption, manufacture difficulty and application popularization prospect etc. etc., these Not small difference, the especially selection of that substrate between material be present, have a great influence.

In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, at this It is extremely simple that conduit is made on the substrate of sample, and the lower cost for material, substrate is made with the polydimethyl siloxane material, Conduit is being suppressed or etched thereon, and is being engaged with the cover plate that the cheap material such as glass or polypropylene or other plastic sheets makes, It is a kind of more satisfactory selection seemingly；Certainly, patch material can also select to use cheap polydimethyl siloxane material： So, this substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, and making is extremely simple, seems and should also be as It is extremely easy to popularize, promotes.

But thing is really not so simple.

First, this polydimethyl siloxane material, that is, the material that alphabetical PDMS is referred to of abridging, itself are a kind of strong Strong hydrophobic material, conduit is built on this material, if without being operated for the modified of the conduit surface, then, it is whole After body assembling is completed, that is, after covering cover plate, because the conduit its inner surface in structure occupies most fluid course Inner surface, then, its strong hydrophobic property of the PDMS conduits inner surface, is deciding factor, it can be similar to water The fine liquid stream of polar liquid of solution by becoming very difficult, its flow resistance is big, or even in general Micropump is all difficult to Promote, certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar；Therefore, existing Among having technology, modification is modified particular for the conduit inner surface on the PDMS material, is necessary operation；So, this The individual modified operation for PDMS conduit inner surfaces is pretty troublesomeThat falls nor this problem, forms serious technical puzzlement, It is another problem：PDMS polymer molecules inside its body phase of this PDMS material substrate have to be diffused to the surface, moves automatically The characteristic of shifting, the characteristic that this substrate body phase inside PDMS polymer molecules are diffused to the surface, migrated automatically, will cause by table State after the modification of its inner surface of that conduit of face modifying and decorating can not maintain the sufficiently long time, and that is through surface Holding time for conduit its inner surface state after modification is substantially only sufficient to the time needs for completing laboratory internal test experiments； In other words, by surface is modified or the PDMS conduit inner surfaces of surface modification, after it is modified or institute's shape after modification is said Into surface state can not be lasting, but soon automatically tend to or say become again surface be modified before surface state, compared with The strong hydrophobic surface state of that script is returned in the short time, then, just think, such micro-fluidic chip can be big Amount making, mass storage, it is widely popularized, answer is it is obvious that is, impossible.Conduit on this PDMS material, does not do If surface modification, it can not pump and pass through similar to the fine liquid stream of polar solvent of the aqueous solution, chip also cannot just use；And such as Fruit has been cooked surface modification, can not persistently keep its state after modifying again, or equally can not popularization and application.

According to the literature, there is a kind of expedient solution, be that a small amount of surface-active is added in sample to be tested solution Agent, so as to which during test, dynamic, temporary transient effective surface hydrophilic layer is built temporarily in the PDMS conduits inner surface； However, due to the amphipathic characteristic of surfactant, the introduced surfactant of the program is inevitable simultaneously also can be with sample solution In tested composition occur combination so that tested composition can not be detected normally or it is detected ratio and dubiously dropped It is low；The surfactant is possibly even fully wrapped around by tested composition with micelle form so that tested composition can not be detected or Identification；Therefore, this kind adds the scheme of surfactant into sample solution, is far from a preferable solution.

So, how to accomplish that substrate can either be made using cheap PDMS material, and the conduit can be released Inner surface decorating state can not persistently, chip can not largely make, largely lay in and then be widely popularized and such a make this area The puzzlement that numerous professionals are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.

Be present many year in the highly difficult problem, so far, not yet properly settled.

Second, the PDMS material of non-surface modification, it is stated that, its surface is strongly hydrophobic above, this strong hydrophobic Material surface and also have another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and And these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into it is gradually deep, until It is heavy to be trapped within the body phase of PDMS substrates, in fact, this process, partly it is also due to PDMS material body phase interior polymer Molecule, which has, to be diffused to the surface, caused by travel motion；Such case, it can also be explained from another angle, i.e. continue not Disconnectedly from inside PDMS body phases to those polymer molecules of its diffusion into the surface, migration, its result moved, be little by little by that It has been involved in a bit by the large biological molecule of adsorption within the body phase of PDMS substrates, briefly, these adsorbed biologies Macromolecular is exactly to be swallowed up by PDMS substrate body phases；So, this PDMS substrates body phase swallows up the phenomenon of large biological molecule, its institute Caused by influence, necessarily cause the severe deviations of all kinds of test data of experiment for being related to large biological molecule.

As described above, the problem of PDMS substrates, is, its not only adsorption large biological molecule, and swallow up biological big point Son, so, as the large biological molecule of experiment test object, its disappearance will not stop because surface saturation is adsorbed, and It is, it is constantly adsorbed, also constantly swallowed up.

On PDMS substrates, its body phase is constantly swallowed up and tests showing for associated biomolecule macromolecular in related experiment test process As, it is to say that another kind, which is explained, substantial amounts of Minute pores in PDMS body phases be present, associated biomolecule macromolecular by after adsorption, Depression enters these Minute pores, and then is swallowed up；However, inventor thinks, those can allow the sky of miniature scale Qi leel squeezes into the Minute pores therebetween, not equal to saying that they also can directly allow the large biological molecule of relative large scale to enter Enter, both difference on yardstick are huge, must not make sweeping generalizations.Explanation is bypassed, in any case, as dependence test analysis object Large biological molecule is adsorbed by PDMS substrate conduits inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is known objective Existing phenomenon.

, can be from containment PDMS surfaces pair in order to prevent swallow up effect of this PDMS substrate bodies relative to large biological molecule The absorption of large biological molecule addresses, and method is chemically modified modification aiming at the PDMS material surface, for For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described channel portion, by chemistry The conduit inner surface of modification, can contain its absorption to large biological molecule, and then avoid large biological molecule quilt PDMS substrate body phases are swallowed up；But or that old problem, that is, the chemical modification on PDMS material surface is modified it Surface state afterwards can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface, migrate automatically Process, can soon by that by surface chemical modification be modified conduit inner surface state become again script it is strong it is hydrophobic simultaneously And the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS substrates its Conduit inner surface is always rapidly to strong hydrophobic surface state evolution.

So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, and can is enough Reach and contain the absorption process of the PDMS substrate conduit inner surfaces to large biological molecule for a long time, and then prevent PDMS substrate bodies relative The effect of swallowing up of large biological molecule so that related chip manufactured goods are able to maintain that a prolonged enough, rational shelf-life, It is exactly a very intractable problem.The problem equally makes this area numerous specially as another problem addressed above Industry personnel are entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.Should Also be present many year in problem, so far, also not yet properly settled.

Third, the polydimethyl siloxane material, namely PDMS material, the active force very little wherein between polymer molecule, The polymer molecule of low polymerization degree inside its body phase is in constantly into the dynamic process of surface migration, therefore, includes PDMS After it has been formed, the surface topography in its inner-walls of duct face of chip can be because of described low polymerization degree polymer point for the chip of substrate material Son moves about, pours into the surface and change, and the change of its pattern is somewhat similarly to Rheological Deformation, trickling deformation or creep and become Pattern caused by shape institute is possible changes；In the higher PDMS material of some low polymerization degree component ratios, this kind somewhat similar Can be showed in the phenomenon of Rheological Deformation, trickling deformation or the deformation of creep must be than more significant；This kind include PDMS substrates chip its The dynamic of inner-walls of duct surface topography after shaping changes property, namely the described property or stream that are somewhat similarly to Rheological Deformation Dropping down the property of deformation or the property of the deformation of creep, the word of rheology one is applied mechanically in this case, integration, also referred to as the rheological equationm of state or rheology become Shape property, the dynamic of corresponding inner-walls of duct face pattern change, and this case is also referred to as rheology；As described above, because of this kind of material Expect certain Rheological Deformation property as described above that its own has, its manufactured goods actually can at leisure Rheological Deformation and cause The manufactured goods total form generation changes；Influence caused by its Rheological Deformation property, it is to be not easy to feel in terms of macroscopic perspective Examine, still, for the very fine micro-fluidic chip of its internal structure, the rheological equationm of state can but cause bigger shadow Ring, the fluid course namely the pipeline therefore, during pre-production formed, because the wall of its tube chamber of pipeline is main It is the wall of PDMS materials, so, the cross-sectional area of the pipeline actually can gradually become because of rheology over time It is small, stated differently, since the reason for the rheology, the natively finer pipeline that is flowed through for liquid sample Its tube chamber the more can become the more narrow, in the case of flow time is sufficiently long, or even the pipeline tube chamber can partly close or all Ground closes, and then make it that sample fluid course is thoroughly blocked, and closes with being even also not reaching to its luminal part of pipeline The degree closed or fully closed, its fluid passage that can be provided of that pipeline narrow because of rheology, then, in pipeline Its inner surface originally be exactly strongly it is hydrophobic in the case of, aqueous sample liquid is just more difficult to by becoming more so because of rheology Narrow micro-channel, then, the influence of substrate rheology how is resisted, maintains the basic framework of the pipeline not occur seriously to become for a long time The problem of shape, also, keep the long-term unobstructed of the pipeline is exactly a urgent need to resolve.

The content of the invention

The technical problem to be solved by the invention is to provide a package solution, solves totally above A series of problems addressed, also, the solution is applied to AIDS diagnosis multichannel micro-fluidic chip field, formed A kind of new AIDS diagnosis Multichannel device.

The present invention solves the technical problem by following scheme, and the device that the program provides is a kind of additional liquid sample The easily-disassembled AIDS diagnosis device of driving building blocks of function, the structure of the device include multichannel micro-fluidic chip, and this is micro- The structure of fluidic chip includes being bonded to each other the substrate and cover plate of installing together, and the substrate and cover plate are plate object or piece The channel structure via mould pressing process or etching technics formation, the base are contained in shape thing, that face towards the cover plate of the substrate Piece also containing be connected with the channel structure and pierce the substrate via mould pressing process, etching technics or simple drilling technology shape Into window structure, be bonded to each other the substrate that is installed together and the cover plate be built into jointly containing pipeline configuration and with Connected liquid pool structure micro-fluidic chip, the locations of structures of the pipeline be located at the interface that the substrate and the cover plate are bonded to each other Region, its side of the window is blocked by the cover plate and opposite side opens, and the locations of structures of the window is exactly the structure of the liquid pool Position, the quantity of the liquid pool is three, and its locations of structures of two liquid pools therein is located at the sample introduction end of the micro-fluidic chip, remaining Under the liquid flowing in its chip when being located at the actual sample introduction test of the micro-fluidic chip of liquid pool its locations of structures terminal, should Terminal is located remotely from each other with the sample introduction end, and one end of the pipeline is branched off via the manifold shape for being similarly positioned in the interface zone being bonded to each other Road respectively with the Liang Ge liquid pools UNICOM positioned at sample introduction end, the other end of the pipeline with positioned at the micro-fluidic chip the terminal A remaining liquid pool UNICOM, and, the working electrode that is sequentially respectively installed in the pipeline on diverse location and right Electrode and reference electrode, the working electrode is by conductive electrode and is attached on the conductive electrode and has embedded AIDS The gold size sensitive membrane of disease-specific antibody is formed, and the construction of the pipeline is in parallel construction, and the pipeline in parallel construction is by four Bar lateral is in parallel to be formed, and the quantity of the working electrode is four, and the installation position of four working electrodes is located at respectively In four laterals, and, the specific antibody difference in its top layer gold size sensitivity membrane structure of four working electrodes It is the four kinds of aids antibody materials that can be specifically bound to AIDS antigen, four kinds of antibody materials are aids antibody respectively P24 and aids antibody gp41 and aids antibody gp120 and aids antibody gp36, its material of the working electrode It is gold material or thermal decomposition conducting polymer material, sheet or thread is presented in its pattern of the working electrode, and emphasis is the base Its material of piece is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, the surface of the primary form its The surface of the primary form for the material for not passing through any surface chemical modification or any surface chemical modification is intended to refer to, The structure of the device also includes chain ring type magnetic holding device, and six links, the ring are shared in its structure of the chain ring type magnetic holding device Section is in strip, shaft-like or bar-shaped, and each adjacent link is hinged to form chain link, the hexagonal ring-type of its appearance profile of the chain link Structure, its locations of structures of each drift angle of the hexagonal annular structure is articulated structure position, the hexagonal annular structure its In two in the link of opposite side position its material be magnetic conductivity material, the remaining four processes of the hexagonal annular structure its Material is non-magnetic material, and the non magnetic material refers to the material not responded strongly to externally-applied magnetic field, the magnetic conductivity Magnet exciting coil is wound with a link among two links of material, this is wound with the link of magnet exciting coil in its excitation wire The pole shoe of a magnetic conductivity material is respectively equiped with the both sides neighbor positions of circle, shares two pole shoes, two pole shoes are equal Point to the link of another magnetic conductivity material, attached on its surface of the link of another magnetic conductivity material it is fixed be equiped with it is miniature Ultrasonic transducer, the chain ring type magnetic holding device socket of electromagnetism adhesive strength is relied on to be positioned at its described end of the micro-fluidic chip The close position at end, and, higher-order of oscillation electric signal transmission cable, one end of the higher-order of oscillation electric signal transmission cable is micro- with this Type ultrasonic transducer links together；The chain ring type magnetic holding device provides a function of facilitating the device to disassemble；This is micro- Its major function of type ultrasonic transducer is in the actual sample introduction test of micro-fluidic chip, utilizes the sample introduction end and the end Difference in the distance between end and the miniature ultrasonic transducer units installation position difference and its ultrasonic intensity experienced Different, the difference between its interfacial tension of sample introduction end described in induced synthesis and the terminal its interfacial tension, the micro-fluidic chip should Interfacial tension difference between both ends can form pressure gap between the both ends of the micro-fluidic chip, and the pressure gap can drive Sample solution is moved to the end flow；It is soft and have the substrate of the dimethyl silicone polymer material of elasticity its function and also include With its property to the strong absorption of ultrasonic wave, ultrasonic wave is absorbed strongly, and thereby in the micro-fluidic chip, the terminal arrives The rapid decrement of ultrasonic intensity is realized within limited short distance between the sample introduction end；And many titanium dioxide Grain, many titanium dioxide granules are filled in the pipeline, and its tube chamber of the pipeline is by many titanium dioxide granules Filled up, for its particle size range of the titanium dioxide granule between 10 microns and 200 microns, the titanium dioxide granule is by mixing The titanium dioxide granule of miscellaneous modification, the titanium dioxide granule are titanium dioxide content its shared percentage by weights more than 80% Particles of inorganic material, the titanium dioxide granule are photic super hydrophilic titanium dioxide granules, and its function of the titanium dioxide granule includes it The titanium dioxide granule surface with super hydrophilic property can be formed by receiving advance radiation of visible light.

As described above, its composition adulterated of the titanium dioxide granule can be tungsten oxide (WO₃), tin oxide (SnO₂), five V 2 O (V₂O₅), iron oxide (Fe₂O₃) zinc oxide (ZnO), the silver (Ag) of reduction-state or two or more above-mentioned doping components Composition；Said composition can be the composition of any ratio of two or more above-mentioned doping components.

Its composition adulterated of the titania particulate material can also include silica (SiO₂)。

Titania particulate material described in this case refers to the photic super hydrophilic titania particulate material of visible light-inducing.

Only with regard to the photic super hydrophilic titanic oxide material of various visible light-inducings for itself, for photic super hydrophilic material It is known for the professional of research field.

Only with regard to the technology of preparing of the photic super hydrophilic titanic oxide material of various visible light-inducings for itself, for photic It is known for the professional in super hydrophilic investigation of materials field.

Its particle diameter of the titanium dioxide granule can be that the basis between 10 microns and 200 microns is actually needed and any The particle diameter specified, such as 10 microns, 30 microns, 70 microns, 100 microns, 150 microns or 200 microns of the particle diameter, etc..

The scope of further preferred its particle diameter of the titanium dioxide granule is between 100 microns and 200 microns.

Its pattern of the titanium dioxide granule is unlimited, and the titanium dioxide granule for example can be spheric granules, rod-shaped particles, length Cube shape particle or any amorphous granular.

The preferred scope of its internal diameter of pipeline is between 500 microns and 1000 microns；It is but excellent compared to above-mentioned The internal diameter of the pipeline scope of choosing, more tiny internal diameter of the pipeline or thicker internal diameter of the pipeline are also what this case was allowed.

The word of magnet exciting coil one art-recognized meanings of itself are known.

The word of magnetic conductivity material one art-recognized meanings of itself are known；The material for belonging to magnetic conductivity material is for example pure Iron, silicon steel, soft magnetic ferrite etc..

Non magnetic material described in this case refers to the material not responded strongly to externally-applied magnetic field, belongs to the non magnetic material The material of matter such as metallic copper, metallic zinc, ormolu, metallic aluminium, almag etc..

Be hinged-word the art-recognized meanings of itself field in Machine Design is known.

The gold size sensitive membrane is to be sufficiently mixed chitosan gold size solution and AIDS specific antibody solution uniformly, is used Point sample instrument point sample is coated on specified structure position, and forms its drying and forming-film.AIDS in the gold size sensitive membrane is special Heterogenetic antibody is the aids antibody of horseradish peroxidase or glucose oxidase mark, and the gold size sensitive membrane has been wrapped Containing complementary medium therein is introduced for the above-mentioned each AIDS specific antibody of fixation, the complementary medium such as shell gathers Sugar, cellulose acetate, gelatin be therein a kind of or their mixture.

The pipeline and the lateral and the manifold shape turnout in the microfluidic chip structure, in it Footpath size may each be arbitrarily selected size, still, for as far as possible less with prepare liquid sample and reduction reagent loss etc. Consideration, the pipeline and the lateral and the manifold shape turnout preferably select capillary level passage, institute The passage for stating capillary level implies that its internal diameter passage suitable with the internal diameter of the capillary on ordinary meaning.The capillary is in it The shape of cross section of portion's passage can be arbitrary shape, and the shape of cross section is for example circular, oval, square, rectangle, bar Shape, naturally it is also possible to it is the linear of bending arbitrarily be present, also, the interior shape of the capillary is with the extension of pipeline, The shape of cross section of different parts can also allow to be different shapes.Only for the word of capillary one, its art-recognized meanings is public Know.

What is be related in structure is the electrode of microsize to electrode and reference electrode, and its electrode shape, which may each be, appoints The selected shape of meaning, the arbitrarily selected shape such as square piece shape, rectangular patch, strip or round sheet etc..It is described Art-recognized meanings to electrode and the reference electrode vocabulary of itself are known.

It is related to several liquid pools in this case microfluidic chip structure, the liquid pool is the pond shape or capsule for transitional liquid storage Shape constructs, and its shape of the inner chamber of each liquid pool may each be arbitrarily selected shape, and the cavity shape is for example round Cylindrical cavity shape, square column type cavity-like, oblong cavity shape or spherical hollow space shape etc..The size of the liquid pool can be any Selected size, still, in order to as far as possible less with prepare liquid sample and reduce reagent loss, the liquid pool be preferably capable of with The liquid pool of the microminiature of capillary matching.

Only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field, It is known.

Various sizes, variously-shaped ultrasonic transducer are commercially available；Its size of commercially available miniature ultrasonic transducer units It may diminish to the magnitude only calculated with millimeter.

Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface its It is known general technology for the professional in ultrasonic technology field for body.This case is not to this expansion superfluous words.

Only with regard to naked PDMS substrates itself conduit molding or lithographic technique for, be open-and-shut known technology； Similarly, the technology of hole-opening is even more known simple technique on naked PDMS substrates.This case is not also to this expansion superfluous words.

The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.

The structure of the device can also include higher-order of oscillation electric signal generator；The higher-order of oscillation electric signal transmission cable Its other end can be connected with the higher-order of oscillation electric signal generator.

The involved higher-order of oscillation electric signal generator technology of itself, come for the professional in ultrasonic technology field Say, be simple and known；The higher-order of oscillation electric signal generator can customize to ultrasonic instrument specialized factory.

The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 5 milliwatts and 9000 milliwatts Between；The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with Between 12MHz.

This case device can further include some annexes certainly, and the annex is such as multiple tracks electrochemical workstation Deng the art-recognized meanings of the multiple tracks electrochemical workstation are known.The each work being related in this case microfluidic chip structure Electrode and to electrode and reference electrode etc., special it can get lines crossed and the multiple tracks electrochemical workstation via corresponding respectively The corresponding interface coupled.It is described that special to get lines crossed be for by each phase of each electrode and the multiple tracks electrochemical workstation Answer the private cable that interface is coupled to each other.The micro-fluidic chip in this case device, its structure can also include micro-valve, The quantity of the micro-valve is unlimited, and according to being actually needed, the micro-valve can be installed in any need in the microfluidic chip structure Position to be mounted；The word of micro-valve one is for the professional of micro fluidic chip technical field, the art-recognized meanings of itself It is known；The micro-valve itself manufacturing technology and the use of technology is also known；The component that the micro-valve is not required.

The diameter of the working electrode can allow to be that any setting is easily installed the suitable diameter used, still, It is recommending or say preferable its scope of the diameter between 0.1 micron to 2000 microns；The length of the working electrode can To allow to be that any setting is easily installed the length used, it is however recommended to or to say the preferable length its scope be 1 Micron is between 15000 microns.

It is installed in by spraying or point sample instrument point sample or the coating of other appropriate process described in the working electrode surface layer Gold size sensitive membrane, its thicknesses of layers can allow be any setting treat sample measuring liquid occur electrical signals response thickness, It is however recommended to thickness preferable thickness is between 10 nanometers and 200 nanometers in other words.

The cover plate in chip structure, its material can allow to be any electrical insulating property material, such as：Polypropylene, Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, for example do Into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and realized in the extremely short distance to ultrasonic wave Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip Using dimethyl silicone polymer it is used as the cover plate, and this case technical scheme is allowed.

Positioned at the sample introduction end of the micro-fluidic chip two liquid pools its with positioned at the micro-fluidic chip terminal should Air line distance between a remaining liquid pool can be arbitrarily selected suitable distance, still, the distance as needed Preferred value is between 3 centimetres and 7 centimetres.

Described its thickness of cover plate and substrate can allow be any setting the thickness for being easy to assembling, the thickness of recommendation or say Preferable thickness is between 1.0 millimeters and 5.0 millimeters.Less thickness is advantageous to save material.

Its maximum difference in application method of this case micro-fluidic chip and general micro-fluidic chip, is exactly this case miniflow Control chip its before biological detection operation is applied to, it is necessary in advance with the radiation of visible light micro-fluidic chip its pipeline The titanium dioxide granule of filling；Because micro-fluidic chip is transparent or translucent state in most cases, and due to The pipeline is fine enough, and its internal titanium dioxide granule filler is also to belong to finer form, it is seen that light also can Inject with enough penetrating or partial penetration inject fine titanium dioxide granule filler its entity of the form, therefore, can at this See in the photic super hydrophilic step of photoinduction, directly with its entirety of the radiation of visible light micro-fluidic chip, it is possible to reach and be directed to Titanium dioxide granule filler inside its pipeline carries out the effect of radiation of visible light；The photic super hydrophilic step of the visible light-inducing Suddenly can be a few minutes or several days before using this case micro-fluidic chip progress biological detection or more than ten days or twenties It is completed.

The light irradiation duration of the radiation of visible light step can be a few minutes to more than ten hour.

The lead of the radiation of visible light step, and, the irradiation duration of the radiation of visible light step is, it is necessary to micro- according to this The species of specific dopant of the titanium dioxide granule filler filled in fluidic chip, the amount of dopant and specific Its parameters of preparation process and its super hydrophilic state formed retain duration to determine.

After described advance radiation of visible light step is passed through, remaining specific detection of the micro-fluidic chip makes With step by hereafter sketching；In other words, hereafter refer to using step and method refer to experienced it is advance described Remaining operation after radiation of visible light.

Itd is proposed first based on this case and the first public new liquid stream driving principle, its application of this case micro-fluidic chip are transported Among work, the new liquid stream driving method is determined completely without involving any additional Micropump.

The interfacial tension difference that this case is formed between micro-fluidic chip both ends caused by the ultrasonic wave, Driving liquid stream flows in the capillary channel of the four-way micro-fluidic chip, right respectively using four-way electrochemical analytical instrument Four kinds of AIDS diagnosis antigens are detected.

The specific detection of this case micro-fluidic chip is as follows using step：

1st, blood serum sample liquid is added in micro-pipe road, under ultrasonic wave driving, various AIDS antigen molecules are each The AIDS specific antibody for the corresponding horseradish peroxidase-labeled that gold size sensitive membrane embeds is caught on electrode surface in passage Obtain.

2nd, the AIDS specific antibody of horseradish peroxidase-labeled is formed with the AIDS antigen in blood serum sample and exempted from Epidemic disease compound.

3rd, using multi-channel electrochemical analyzer, the electron mediators such as catechol are added, it is above-mentioned using amperometric detection Curent change caused by reaction, it is derived from the species and content of various analytes.

4th, result is subjected to comprehensive analysis, comprehensive diagnos is carried out to AIDS antigen.

It is an advantage of the invention that its close position relies on electromagnetism adhesive strength set in the terminal of the micro-fluidic chip The described chain ring type magnetic holding device of positioning is connect, it is miniature super to attach installation in the chain ring type magnetic holding device one magnetic conductivity link Acoustic wave transducer, with the ultrasonic wave of miniature ultrasonic transducer units transmitting low-power, high-frequency band, meanwhile, utilize poly dimethyl Its strong absorbability to ultrasonic wave of siloxanes substrate, in shorter distance, it is, from the terminal to it is described enter In the very short distance of only several centimeters yardsticks between sample end, reach the rapid decrement of ultrasonic intensity, it is thereby micro-fluidic at this The difference of the interfacial tension is caused at the both ends of chip, and then, utilize its institute of the difference of the interfacial tension between the both ends Pressure gap between the both ends formed, driving sample liquid stream flow in the pipeline to the terminal direction.By this Case liquid stream drive scheme, has altogether dispensed with the equipment of traditional Micropump etc；The micro-fluidic chip of Micropump is eliminated, its Structure is more succinct, and its production process is less, and its cost of manufacture is lower, and this kind of succinct structure is more beneficial for reaching micro-fluidic chip Low cost application.

Its scheme of this case is all to use all clearance spaces in the pipeline in addition to electrode installation takes up space The titanium dioxide granule fills up.Those are filled in the titanium dioxide granule in pipeline, and this case is to its overall abbreviation titanium dioxide Granule filler, its function include support, withstand the tube chamber of the pipeline its inwall so that the tube chamber of the pipeline is from because described in Rheology and narrow, prevent its reduced cross-sectional area of the tube chamber of the pipeline or even close, so as to maintaining the basic of the pipeline for a long time Serious deformation does not occur for framework.

Numerous titanium dioxide granule entities are mutually banked up under this case framework together at random in the pipeline, the titanium dioxide The function of titanium granule filler, having for the titanium dioxide granule for also including living through advance radiation of visible light with it certainly are super The particle surface of hydrophilic nmature, utilize the mutual institute that is mutually close together by ultra-hydrophilic surface of each adjacent titanium dioxide granule The complications of formation are continuous and its inner surface has the space of super hydrophilic property, in the pipeline Inner Constitution network morphology Fluid channel with super hydrophilic property, although the fluid channel with super hydrophilic property that this complications can be continuous all the time, So, inside the tube chamber of the pipeline simultaneously and a plurality of fluid channel with super hydrophilic property deposited its integrate, The effect of integrated, cumulative superposition in other words, is the equal of that the relatively fine capillary with Superhydrophilic of caliber leads to Road；Its presence of the continuous super hydrophilic fluid channel of the complications, its Superhydrophilic to a large extent in other words completely, Thoroughly overcome, surmounted in degree, counteracting the strong hydrophobic property in its surface of the PDMS substrates of the primary form and made Into the flow resistance for liquid sample；In other words, it is water that because of liquid sample, it, which accounts for the composition of maximum ratio, liquid sample It is exactly substantially the aqueous solution, therefore, the presence of the titanium dioxide granule filler can be significantly complete, thorough in other words Overcome in the degree at bottom with the unmodified PDMS substrates of hydrophobic property and surface its to the aqueous solution it is incompatible, repel and Barrier effect, so be greatly reduced belong to properties of Aqueous Solution sample liquid stream its be advanced through the resistance of pipeline, its flow Dynamic resistance be possibly even less than or well below the aqueous solution similar caliber the unorganic glass using silica as main component Flow resistance in capillary.

Under this case framework, inside the tube chamber of the pipeline, the area of its lumen wall wall of former pipeline, because of described two The rigid of titan oxide particles filler ties up, the table of part hydrophobic property that still can also be exposed on lumen wall wall Its area of face is already dramatically reduced, and the area on its still exposed part surface for belonging to hydrophobic property is much Less than the wall surface area of the hydrophobic property of the lumen wall wall in the presence of no titanium dioxide granule filler； So, under this case framework, in its lumen of the pipeline, its remaining hydrophobic surface and formed after advance radiation of visible light Super hydrophilic part increases surface newly, and that it is superimposed, comprehensive, summation, cumulative technique effect, is to form on the whole In super hydrophilic surface；In other words, inside the tube chamber of the pipeline, the part surface of remaining hydrophobic property its with examination Interfacial tension between sample solution, plus the super hydrophilic part formed after advance radiation of visible light increase newly surface its with Interfacial tension between sample solution, that it is superimposed, comprehensive, summation, cumulative technique effect, is so that the pipeline The synergy of its solid-liquid interfacial tension levels off between its tube chamber inner surface of capillary glass tube and sample solution inside tube chamber Interfacial tension, it is even lower；In other words, as the above-mentioned interfacial tension in this case of the cumulative technique effect actually Be level off to the aqueous solution and with super hydrophilic property the surface of solids between that interface interfacial tension.

Therefore, exist in the pipeline under this case framework for having stated titanium dioxide granule filler, in advance to core In the case that its titanium dioxide filler of piece has carried out radiation of visible light, in other words, it is, because micro-fluidic chip is general For transparent or semitransparent state is integrally presented, therefore, in the case where having carried out radiation of visible light to chip in advance, based on this The new liquid stream drive scheme of case, it becomes possible to reached with extremely low ultrasonic power by the actuation techniques scheme of this case to examination The driving of sample liquid stream.

On the other hand, due to its rheological equationms of state of PDMS as substrate material, it is filled in the pipeline The titanium dioxide granule filler, can gradually by constantly Rheological Deformation and to arest neighbors free space promote PDMS materials Pipeline wall more tightly wrap up, this process eventually causes those part titanium dioxides for pressing close to the lumen wall wall Titanium particle is stuck in original place or is stuck in original place or embedding in-situ titanium dioxide granule and remaining embedded in original place, the part The titanium dioxide granule banked up with being mutually closely packed together each other incarceration together, due to this reason, the dioxy Change titanium granule filler just will not move in the pipeline easily, and titanium dioxide granule therein is also substantially all locked In original place, therefore the stream framework of the micro-fluidic chip can be kept for a long time.

The particle size range of its particle of this case titanium dioxide granule filler between 10 microns and 200 microns, compared to For general organic molecule, general large biological molecule, the particle diameter of its particle of this case titanium dioxide granule filler can be rated as huge Greatly, due to its huge particle diameter, and the inner surface of the surface of its polarity and the pipeline of strong hydrophobic PDMS materials can not Mutually perhaps say and mutually melt, then, the huge titanium dioxide granule of this kind of particle diameter can not be by the pipeline of PDMS materials Surface is adsorbed, and can not more be swallowed up by the inner surface of the pipeline of PDMS materials, the huge titanium dioxide of this kind of particle diameter Titanium particle surely not be fully sunk in easily or sink to completely PDMS materials its among intrinsic many Minute pores, and Because above it is stated that the rheology the reason for, the titanium dioxide granule filler can be stuck in original place in other words it is embedding In original place without moving easily.

Inside the tube chamber of the pipeline, its lumen wall wall of former pipeline is entirely the surface of hydrophobic property originally, such as Upper described, under this case framework, rigid because of the titanium dioxide granule filler ties up, on lumen wall wall still also Its area of the surface of part hydrophobic property that can be exposed is already dramatically reduced, and it is still exposed to belong to hydrophobicity The area on the part surface of matter has been far smaller than the tube chamber in the presence of no titanium dioxide granule filler The wall surface area of the hydrophobic property of inwall wall；The reason for because of the rheology, it is close to those dioxies of lumen wall wall Change titanium particle, be little by little partially embedded into or be partly absorbed among the lumen wall wall, the dioxy among the state Changing titanium particle, it towards the part surface of lumen wall wall and has occurred and that the lumen wall wall that matching deforms is tight Closely connected conjunction, then, the matching is deformed and closely pasted with titanium dioxide granule surface having occurred and that in lumen wall wall The part wall of conjunction actually can not contact with flowing through the sample solution of pipeline, that is to say, that the wall base of this part In sheet can not suction-operated be produced to the large biological molecule therein that flows through the sample solution of pipeline and organic molecule.Base In above-mentioned mechanism, then because fill up the pipeline the titanium dioxide granule filler its presence, its tube chamber of the pipeline The overwhelming majority of inwall wall is tightly covered, and these described walls tightly covered substantially can not be with Flow through the pipeline sample solution contact, only remaining fraction by titanium dioxide granule, its surface is not pasted closely The hydrophobic surface close, tightly covered may contact with sample solution, in other words, under this case framework, the life in sample solution Its direct touch opportunity with hydrophobic PDMS walls of thing macromolecular and organic molecule is significantly reduced, thus, PDMS materials Its adsorptive hindrance and interference of swallowing up to large biological molecule and organic molecule of lumen wall face is greatly reduced；As above institute State, this case scheme is advantageous to exclude the adsorptive hindrance and swallows up interference, is advantageous to lift the reliable of correlation analysis test data Property.

As described above, this case titanium dioxide granule filler, its function actually includes being similar to being directed to PDMS materials Inner surface of pipeline carry out chemical modification effect, functioning as its this respect is by the inner surface of pipeline of the PDMS materials By the surface modification of hydrophobic property into the surface of super hydrophilic property, still, with general, usual PDMS surface chemical modifications its The hydrophilic chemical decorative layer retention time is short, hydrophilic effect can not keep situation prolonged enough different, particle described in this case The huge titanium dioxide granule filler in footpath, its particle being stuck by rheology can not both be moved easily, its huge particle diameter Particle can not more be swallowed up easily by the inner-walls of duct of PDMS materials, therefore, its from this respect changes similar to surface chemistry Seen on the technique effect of property, its technique effect of this case, be form it is permanent, can not erase, can not consume, not be corroded, no The surface reforming layer with super hydrophilic property being swallowed up, being not dissolved, it is just comparable to one kind in PDMS in effect The permanent modified surface layer with super hydrophilic property built in its relevant surfaces of base material.

It is related that the technical scheme of this case has dissolved its application to dimethyl silicone polymer substrate addressed above totally A series of technical barriers.Based on this case scheme, the very cheap polydimethyl siloxane material of this kind is just possible to micro- at this It is prepared by fluidic chip, production, using etc. field play bigger effect.

Attached in the chain ring type magnetic holding device one magnetic conductivity link in this case structure it is fixed installed it is described miniature Ultrasonic transducer, this architecture provides a function of facilitating the device to disassemble, in this way, the chain ring type magnetic holding device is together with it The miniature ultrasonic transducer units attached in one magnetic conductivity link just easily can be mutually disengaged with the micro-fluidic chip, that , the component that the part can freely depart from reuses many times in which just can circulate benignly；The architectural feature is advantageous to save The use cost of the device.

Brief description of the drawings

Fig. 1 is the schematic diagram of this case device embodiment chip part construction, it is shown that the vertical view of this structure The structural form of chip internal pipeline and each electrode and each related liquid pool under angle, the chain ring type is not depicted in the figure Magnetic holding device and the miniature ultrasonic transducer units set up thereon and other annexes.

Fig. 2 be this case device chain ring type magnetic holding device its with appearance side rough under the micro-fluidic chip socket state View, the legend and indicates the relative position relation under the socket state between each component.

Fig. 3 is the outside side view under another viewing angle of this case device；Fig. 3 viewing angle and Fig. 2's Viewing angle is mutually perpendicular to.

In figure, 1,2,10 be the different liquid pool of three installation positions respectively, and 3 be manifold shape turnout, and 4,7,11,14 are respectively Installation position is different but four laterals parallel with one another for forming UNICOM's structure in parallel, and 5 be its that be installed in lateral 4 The working electrode that specific antibody material in the gold size sensitivity membrane structure of top layer is AIDS specific antibody p24,6 be to be installed in The work that the specific antibody material in its top layer gold size sensitivity membrane structure in lateral 7 is AIDS specific antibody gp41 Make electrode, 12 be that specific antibody material in its top layer gold size sensitivity membrane structure being installed in lateral 11 is AIDS Specific antibody gp120 working electrode, 13 be the spy in its top layer gold size sensitivity membrane structure being installed in lateral 14 Heterogenetic antibody material is AIDS specific antibody gp36 working electrode, and 8 be to electrode, and 9 be reference electrode, and 15 be chain ring type Magnetic holding device, 16 be the terminal of the micro-fluidic chip, and 17 be the sample introduction end of the micro-fluidic chip, and 18 be poly dimethyl The substrate of siloxanes material, 19 be cover plate, and 20 be miniature ultrasonic transducer units, and 21 be higher-order of oscillation electric signal transmission cable, 22, 29 be the link of two magnetic conductivity materials positioned at different structure position respectively, and the link of two magnetic conductivity materials is in the structure In end-to-end relation each other, 23,32 be two pole shoes positioned at different structure position respectively, and 24,26,28,31,34,36 are respectively Six pin joints in six diverse locations, 25,27,33,35 be four non magnetic materials in four diverse locations respectively The link of matter, 30 be magnet exciting coil；The chain ring type magnetic holding device structure in legend is only the legend structure of signal, actually the chain Ring type magnetic holding device structure is not limited to the legend chain ring type magnetic holding device structure；Arrow in legend indicates the micro-fluidic core Piece its in actual motion, by pressure at two ends difference drive, the flow direction of its sample liquid stream.

Embodiment

In this case that Fig. 1, Fig. 2 and Fig. 3 are shown embodiment, the structure of the device includes multichannel micro-fluidic core Piece, the structure of the micro-fluidic chip include being bonded to each other the substrate 18 and cover plate 19 of installing together, the substrate 18 and cover plate 19 be plate object or tablet, and that face towards the cover plate 19 of the substrate 18 is contained via mould pressing process or etching technics The channel structure of formation, the substrate 18 also containing be connected with the channel structure and pierce the substrate via mould pressing process, quarter The window structure that etching technique or simple drilling technology are formed, is bonded to each other the substrate 18 being installed together and the cover plate 19 is common Be built into the micro-fluidic chip containing pipeline configuration and the liquid pool structure being attached thereto, the liquid pool be liquid pool 1, liquid pool 2, Liquid pool 10, the locations of structures of the pipeline are located at the interface zone that the substrate 18 is bonded to each other with the cover plate 19, its side of the window Blocked and opposite side opening by the cover plate 19, the locations of structures of the window is exactly the liquid pool 1, liquid pool 2, the structure bit of liquid pool 10 Put, the quantity of the liquid pool is three, and two liquid pools therein are that liquid pool 1 and liquid pool 2 its locations of structures are located at the micro-fluidic core The sample introduction end 17 of piece, a remaining liquid pool be when liquid pool 10 its locations of structures is located at the micro-fluidic chip actual sample introduction test its The terminal 16 that liquid flows in chip, the terminal 16 is located remotely from each other with the sample introduction end 17, and one end of the pipeline is via being similarly positioned in The manifold shape turnout 3 of the interface zone being bonded to each other, should respectively with the liquid pool 1 positioned at sample introduction end 17 and the UNICOM of liquid pool 2 The other end of pipeline and a remaining liquid pool for the terminal 16 positioned at the micro-fluidic chip are the UNICOM of liquid pool 10, with And sequentially it is respectively installed in working electrode in the pipeline on diverse location and to electrode 8 and reference electrode 9, the work It is sensitive by conductive electrode and the gold size for having embedded AIDS specific antibody being attached on the conductive electrode to make electrode Film is formed, and the construction of the pipeline is in parallel construction, and the pipeline in parallel construction is made up of four lateral parallel connections, described The quantity of working electrode is four, and the installation position of four working electrodes is respectively in four laterals, and this four Individual working electrode is working electrode 5, working electrode 6, working electrode 12, working electrode 13 respectively, and, four working electrodes Specific antibody in its top layer gold size sensitivity membrane structure is the four kinds of AIDS that can be specifically bound to AIDS antigen respectively Antibody materials, four kinds of antibody materials are aids antibody p24 and aids antibody gp41 and aids antibody respectively Gp120 and aids antibody gp36, for specifically deploying, 5 be its top layer gold size sensitive membrane being installed in lateral 4 The working electrode that specific antibody material in structure is AIDS specific antibody p24,6 are installed in lateral 7 The working electrode that specific antibody material in its top layer gold size sensitivity membrane structure is AIDS specific antibody gp41,12 be dress Specific antibody material in its top layer gold size sensitivity membrane structure being located in lateral 11 is AIDS specific antibody Gp120 working electrode, 13 be the specific antibody thing in its top layer gold size sensitivity membrane structure being installed in lateral 14 Matter is AIDS specific antibody gp36 working electrode, and the working electrode 5, working electrode 6, working electrode 12, work are electric Its material of pole 13 is gold material or thermally decomposes conducting polymer material, the working electrode 5, working electrode 6, working electrode 12nd, sheet or thread is presented in its pattern of working electrode 13, and emphasis is, its material of the substrate 18 is dimethyl silicone polymer material, Its surface of substrate 18 is the surface of primary form, the surface of the primary form its be intended to refer to and do not come to the surface by any The surface of the primary form of the material of modification or any surface chemical modification is learned, the structure of the device also includes chain ring type electromagnetism Fixture 15, shares six links 22,25,27,29,33,35 in its structure of the chain ring type magnetic holding device 15, the link 22,25, 27th, 29,33,35 in strip, shaft-like or bar-shaped, and each adjacent link is hinged to form chain link, and its appearance profile of the chain link is in Hexagonal annular structure, each drift angle of the hexagonal annular structure are that drift angle 24,26,28,31,34,36 its locations of structures is equal For articulated structure position, two therein links in opposite side position of the hexagonal annular structure are its material of link 22,29 Magnetic conductivity material, the remaining four processes of the hexagonal annular structure be link 25,27,33,35, its material be non magnetic Material, the non magnetic material refer to the material not responded strongly to externally-applied magnetic field, two links of the magnetic conductivity material A link i.e. among link 22,29 is that magnet exciting coil 30 is wound with link 29, and this is wound with the link of magnet exciting coil 30 29 are respectively equiped with the pole shoe of a magnetic conductivity material in the both sides neighbor positions of its magnet exciting coil 30, share two poles Boots, the pole shoe are pole shoe 23 and pole shoe 32, and two pole shoes are that pole shoe 23 and pole shoe 32 are directed to another magnetic conductivity material Link 22, fixation is attached on its surface of the link 22 of another magnetic conductivity material and is equiped with miniature ultrasonic transducer units 20, The chain ring type magnetic holding device 15 socket of electromagnetism adhesive strength is relied on to be positioned at the neighbouring of the micro-fluidic chip its terminal 16 Position, and, higher-order of oscillation electric signal transmission cable 21, one end of the higher-order of oscillation electric signal transmission cable 21 is miniature super with this Acoustic wave transducer 20 links together；The chain ring type magnetic holding device 15 provides a function of facilitating the device to disassemble；This is micro- Its major function of type ultrasonic transducer 20 is in the actual sample introduction test of micro-fluidic chip, utilizes the sample introduction end 17 and institute State the distance between terminal 16 and the installation position of miniature ultrasonic transducer units 20 difference and its ultrasonic wave experienced is strong Difference between difference on degree, its interfacial tension of sample introduction end 17 described in induced synthesis and the terminal 16 its interfacial tension, should The micro-fluidic chip both ends are that the interfacial tension difference between sample introduction end 17 and terminal 16 can be at the both ends of the micro-fluidic chip Pressure gap is formed between sample introduction end 17 and terminal 16, the pressure gap can drive sample solution to be flowed to the direction of terminal 16 It is dynamic；It is soft and have the substrate 18 of the dimethyl silicone polymer material of elasticity its function and include with it to the strong absorption of ultrasonic wave Property, ultrasonic wave is absorbed strongly, and it is thereby limited between micro-fluidic chip terminal 16 to the sample introduction end 17 Short distance within realize the rapid decrement of ultrasonic intensity；And many titanium dioxide granules, many titanium dioxide It is particles filled in the pipeline, its tube chamber of the pipeline is filled up by many titanium dioxide granules, the titanium dioxide For its particle size range of grain between 10 microns and 200 microns, the titanium dioxide granule is the titanium dioxide by doping vario-property Grain, the titanium dioxide granule are its particles of inorganic material of shared percentage by weight more than 80% of titanium dioxide content, the dioxy It is photic super hydrophilic titanium dioxide granule to change titanium particle, and its function of the titanium dioxide granule includes it can be by receiving in advance Radiation of visible light forms the titanium dioxide granule surface with super hydrophilic property.

Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, its try The flow direction of sample liquid stream.

The chain ring type magnetic holding device 15 is depicted without in Fig. 1 and attaches fixation in one magnetic conductivity material link Miniature ultrasonic transducer units 20 and higher-order of oscillation electric signal transmission cable 21 of installing；Also, in Fig. 1, Fig. 2 and Fig. 3 It is depicted without the associate members such as the higher-order of oscillation electric signal generator and multiple tracks electrochemical workstation.

Involved magnetic conductive material and involved nonmagnetic substance market are on sale.

Involved miniature ultrasonic transducer units 20 are commercially available；It can also be customized to ultrasonic transducer producer.

Involved higher-order of oscillation electric signal transmission cable 21 is commercially available；Can also be special to ultrasonic transducer producer or cable Industry producer customizes.

Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available；It can also determine to relevant manufacturers System.

Each working electrode in this example structure and can be respectively via each special electricity to electrode and reference electrode Cable or say is being got lines crossed respectively with the corresponding cable interface of the multiple tracks electrochemical workstation as annex or saying interface connection of getting lines crossed.

Claims (10)

1. the easily-disassembled AIDS diagnosis device of additional liquid sample driving building blocks of function, the structure of the device includes leading to more Road micro-fluidic chip, the structure of the micro-fluidic chip include being bonded to each other the substrate and cover plate of installing together, the substrate and Cover plate is plate object or tablet, and that face towards the cover plate of the substrate is contained via mould pressing process or etching technics shape Into channel structure, the substrate also containing be connected with the channel structure and pierce the substrate via mould pressing process, etch work The window structure that skill or simple drilling technology are formed, it is bonded to each other the substrate being installed together and is built into jointly with the cover plate Micro-fluidic chip containing pipeline configuration and the liquid pool structure being attached thereto, the locations of structures of the pipeline are located at the substrate with being somebody's turn to do The interface zone that cover plate is bonded to each other, its side of the window is blocked by the cover plate and opposite side opens, the locations of structures of the window It is exactly the locations of structures of the liquid pool, the quantity of the liquid pool is three, and it is micro- that its locations of structures of two liquid pools therein is located at this The sample introduction end of fluidic chip, its locations of structures of a remaining liquid pool are located at its chip when the actual sample introduction of the micro-fluidic chip is tested The terminal of interior liquid flowing, the terminal are located remotely from each other with the sample introduction end, and one end of the pipeline is bonded to each other via this is similarly positioned in Interface zone manifold shape turnout respectively with the Liang Ge liquid pools UNICOM positioned at sample introduction end, the other end of the pipeline with positioned at should A remaining liquid pool UNICOM for the terminal of micro-fluidic chip, and, sequentially it is respectively installed in different positions in the pipeline The working electrode put and to electrode and reference electrode, the working electrode is by conductive electrode and is attached to the conduction Property electrode on embedded AIDS specific antibody gold size sensitive membrane form, the construction of the pipeline is in parallel construction, described It is made up of in the pipeline of parallel construction four lateral parallel connections, the quantity of the working electrode is four, this four work electricity The installation position of pole is located in four laterals respectively, and, its top layer gold size sensitive membrane knot of four working electrodes Specific antibody in structure is the four kinds of aids antibody materials that can be specifically bound to AIDS antigen respectively, four kinds of antibody Material is aids antibody p24 and aids antibody gp41 and aids antibody gp120 and aids antibody respectively Gp36, its material of the working electrode is gold material or thermally decomposes conducting polymer material, and its pattern of the working electrode is in Existing sheet or thread, it is characterised in that its material of the substrate is dimethyl silicone polymer material, and its surface of the substrate is primary shape The surface of state, the surface of the primary form its be intended to refer to and do not change by any surface chemical modification or any surface chemistry The surface of the primary form of the material of property, the structure of the device also include chain ring type magnetic holding device, the chain ring type magnetic holding device Sharing six links in its structure, in strip, shaft-like or bar-shaped, each adjacent link is hinged to form chain link the link, The hexagonal cyclic structure of its appearance profile of the chain link, its locations of structures of each drift angle of the hexagonal annular structure is be hinged Locations of structures, in the link of opposite side position, its material is magnetic conductivity material to two therein of the hexagonal annular structure, and this six Its material of the remaining four processes of side shape cyclic structure is non-magnetic material, and the non magnetic material is referred to externally-applied magnetic field The material not responded strongly, magnet exciting coil is wound with a link among two links of the magnetic conductivity material, this is twined The link for being wound with magnet exciting coil is respectively equiped with the pole shoe of a magnetic conductivity material in the both sides neighbor positions of its magnet exciting coil, altogether There are two pole shoes, two pole shoes are directed to the link of another magnetic conductivity material, the ring of another magnetic conductivity material Save and fixed chain ring type magnetic holding device set for being equiped with miniature ultrasonic transducer units, relying on electromagnetism adhesive strength is attached on its surface The close position for being positioned at its terminal of the micro-fluidic chip is connect, and, higher-order of oscillation electric signal transmission cable, the high frequency vibrating The one end and the miniature ultrasonic transducer units for swinging electric signal transmission cable link together；The chain ring type magnetic holding device provides one The individual function of facilitating the device to disassemble；Its major function of the miniature ultrasonic transducer units is in the actual sample introduction test of micro-fluidic chip When, using the sample introduction end and the distance between the terminal and the miniature ultrasonic transducer units installation position difference and its The difference on ultrasonic intensity experienced, its interfacial tension of sample introduction end described in induced synthesis and its interfacial tension of the terminal Between difference, interfacial tension difference between the micro-fluidic chip both ends can between the both ends of the micro-fluidic chip shape Into pressure gap, the pressure gap can drive sample solution to the end flow；The poly dimethyl silicon that is soft and having elasticity Its function of the substrate of oxygen alkane material is also included with its property to the strong absorption of ultrasonic wave, and ultrasonic wave is absorbed strongly, and Thereby the quick of ultrasonic intensity is realized within micro-fluidic chip terminal to the limited short distance between the sample introduction end Successively decrease；And many titanium dioxide granules, many titanium dioxide granules are filled in the pipeline, the pipeline its Tube chamber is filled up by many titanium dioxide granules, and its particle size range of the titanium dioxide granule is between 10 microns and 200 microns Between, the titanium dioxide granule is the titanium dioxide granule by doping vario-property, and the titanium dioxide granule is titanium dioxide content Its particles of inorganic material of shared percentage by weight more than 80%, the titanium dioxide granule are photic super hydrophilic titanium dioxide Grain, its function of the titanium dioxide granule, which includes it, to be formed by receiving advance radiation of visible light with super hydrophilic property Titanium dioxide granule surface.

2. the easily-disassembled AIDS diagnosis device of additional liquid sample driving building blocks of function according to claim 1, Characterized in that, the pipeline and the lateral and the manifold shape turnout are capillary channel.

3. the easily-disassembled AIDS diagnosis device of additional liquid sample driving building blocks of function according to claim 1, Characterized in that, its composition adulterated of the titanium dioxide granule is tungsten oxide (WO₃), tin oxide (SnO₂), vanadic anhydride (V₂O₅), iron oxide (Fe₂O₃) zinc oxide (ZnO), the silver (Ag) of reduction-state or two or more above-mentioned doping components combination Thing.

4. the easily-disassembled AIDS diagnosis device of additional liquid sample driving building blocks of function according to claim 1, Characterized in that, the width or diameter of the working electrode be between 0.1 micron to 2000 microns, and, the work electricity The length of pole is between 1 micron to 15000 microns.

5. the easily-disassembled AIDS diagnosis device of additional liquid sample driving building blocks of function according to claim 1, Characterized in that, the thickness of the gold size sensitive membrane is between 10 nanometers and 200 nanometers.

6. the easily-disassembled AIDS diagnosis device of additional liquid sample driving building blocks of function according to claim 1, Characterized in that, the cover plate its material in structure is dimethyl silicone polymer material.

7. the easily-disassembled AIDS diagnosis device of additional liquid sample driving building blocks of function according to claim 1, Characterized in that, positioned at the sample introduction end of the micro-fluidic chip two liquid pools its with positioned at the micro-fluidic chip terminal Air line distance between a remaining liquid pool is between 3 centimetres and 7 centimetres；It is thick for the cover plate and substrate in structure Degree is between 1.0 millimeters and 5.0 millimeters.

8. the easily-disassembled AIDS diagnosis device of additional liquid sample driving building blocks of function according to claim 1, Characterized in that, its particle size range of the titanium dioxide granule is between 100 microns and 200 microns, its internal diameter model of the pipeline Enclose between 500 microns and 1000 microns.

9. the easily-disassembled AIDS diagnosis device of additional liquid sample driving building blocks of function according to claim 1, Characterized in that, the structure of the device also includes higher-order of oscillation electric signal generator, the higher-order of oscillation electric signal transmission cable Its other end is connected with the higher-order of oscillation electric signal generator.

10. the easily-disassembled AIDS diagnosis device of additional liquid sample driving building blocks of function according to claim 1, Characterized in that, its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units, between 5 milliwatts and 9000 milliwatts, this is micro- The frequency of its ultrasonic wave operationally launched of type ultrasonic transducer is between 100KHz and 12MHz.