CN107537044A - A kind of chitosan nano microvesicle and preparation method thereof and purposes - Google Patents
A kind of chitosan nano microvesicle and preparation method thereof and purposes Download PDFInfo
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Abstract
The present invention, which provides a kind of chitosan nano microvesicle and preparation method thereof and purposes, this method, to be included：(1) acrylic acid aqueous solution is prepared; chitosan is added, after dissolving, solution is heated to 60 80 DEG C in the presence of protective gas; add initiator for reaction; then solution temperature is reduced and reacted, after terminating, filter and dialyse remove impurity; after processing terminates; crosslinking agent is added, heating, reacts and nanoparticle suspension is made；(2) freeze drying protectant is added into the nanoparticle suspension, be freeze-dried, the chitosan nano microvesicle is made.Chitosan is dissolved in acrylic acid solution by the present invention by a certain percentage, after addition initiator makes acrylic acid obtain the hollow Nano grain of inner aqueous with chitosan generation polymerisation, remove the moisture of boring structure, after obtained nanometer microvesicle dissolves again, there is the synergistic effect of ultrasonic development and high intensity focused ultrasound ablation of tissue.
Description
Technical field
The present invention relates to contrast preparation field, more particularly to a kind of chitosan nano microvesicle and preparation method thereof and purposes.
Background technology
Acoustic contrast agent more ripe at present is micron order contrast preparation, and about 2-8 μm of diameter range, they can be passed through
Pulmonary capillaries, do not cause embolism, but can not pass through outside blood vessel, limit its development capability to disease outside blood vessel, Er Qieyan
Also relatively narrower, micron order acoustic contrast agent can not pass through sometimes for disease and embolism position blood vessel.With molecular biology and receive
The development of rice technology, nanometer pharmacy emerge rapidly.Study on nano-scale contrast agents grain size, can be effectively real often in 700nm
Develop outside existing blood vessel, advantage is had more compared with micron order microvesicle in terms of clinical diagnosis and treatment, turn into a direction of microvesicle research.
At present, nanoscale ultrasound contrast agents have the nano-particle of coating solid particle, the negative liquid fluorocarbon of bag nanoemulsion,
The nanometer microvesicle for body of doing something in a fit of pique is wrapped, because of preparation method, filmogen and contains content difference, prepared contrast agent particle diameter difference
Larger, imaging effect is also different.Currently used high polymer material has Poly(D,L-lactide-co-glycolide (PLGA), state
Interior existing scholar uses Poly(D,L-lactide-co-glycolide to prepare macromolecule polymer microvesicle for sheathing material, but can prepare
Minimum grain size can only arrive 600nm or so.Foreign countries have scholar to prepare PLGA nanometer microvesicles, and average grain diameter reaches 150-200nm, but quiet
The echo of the reagent is weak after arteries and veins injection, and ultrasonic examination can not be imaged, it is seen that PLGA study on nano-scale contrast agents is used for the related skill developed
Art is not yet ripe.
There is research to use nanoscale silica granule to prepare chitosan nano microvesicle for template at present, in chitosan hollow Nano
After being filled with perfluor pentane in grain, ultrasonic imaging can be realized, but the preparation process is complicated, cost is high.
The content of the invention
In view of the above the shortcomings that prior art, it is an object of the invention to provide a kind of chitosan nano microvesicle and its
Preparation method and purposes, for solving the preparation process of nanometer microvesicle in the prior art is complicated, cost is higher, imaging effect is poor etc.
Problem.
In order to achieve the above objects and other related objects, the present invention provides a kind of preparation method of chitosan nano microvesicle,
Comprise the following steps：
(1) acrylic acid aqueous solution is prepared, chitosan is added, after dissolving, is heated to solution in the presence of protective gas
60-80 DEG C, initiator for reaction 10min-60min is added, solution temperature is then reduced to 50 DEG C -65 DEG C, reacts 60-
120min, reaction terminate after, filter and dialyse remove impurity, after processing terminates, add crosslinking agent, heating, react be made nanometer
Grain suspension；
(2) freeze drying protectant is added into the nanoparticle suspension, be freeze-dried, it is micro- that the chitosan nano is made
Bubble.
Further, in step (1), the mass concentration of the acrylic acid aqueous solution is 0.1-1.6%.
Preferably, in step (1), the mass concentration of the acrylic acid aqueous solution is 0.22-0.44%.
Further, in step (1), (unit g) is acrylic acid aqueous solution volume (Unit/mL) to the chitosan mass of addition
0.5-3.5%.
Preferably, in step (1), (unit g) is acrylic acid aqueous solution volume (Unit/mL) to the chitosan mass of addition
0.5-1%.
Further, in step (1), the molecular weight of chitosan is 100kDa-800kDa, deacetylation 60%-
100%.
Further, in step (1), the initiator is selected from potassium peroxydisulfate.
Further, in step (1), the addition of initiator is 30mg-70mg, and now the volume of acrylic acid aqueous solution is
50mL。
Further, in step (1), the protective gas is selected from nitrogen or other inert gases.
Further, in step (1), solution is heated to 70-75 DEG C in the presence of protective gas.
Further, in step (1), the crosslinking agent is selected from least one of glutaraldehyde, sodium tripolyphosphate.
Further, in step (1), the bag filter equipped with reaction solution is placed in buffer solution, dialysis removes impurity.
Further, in step (1), the buffer solution is selected from acetic acid-acetate buffer, pH 4.5.
Further, in step (1), processing time of the nanoparticle suspension in buffer solution is 24-48h.
Further, in step (1), add crosslinking agent quality (unit g) be reaction solution volume (Unit/mL) 0.05-
2%.
Further, in step (1), after adding crosslinking agent, solution is heated to 40 DEG C of -50 DEG C of reactions.
Further, in step (1), after adding crosslinking agent, reaction time 1h-2h.
Further, in step (2), the freeze drying protectant in mannitol, glucose, sucrose, lactose at least
It is a kind of.
Further, in step (2), adding the quality of freeze drying protectant, (unit g) is nanoparticle suspension volume (unit
ML 1%-10%).
Further, in step (2), adding the quality of freeze drying protectant, (unit g) is nanoparticle suspension volume (unit
ML 1-5%).
Further, in step (2), freeze-drying method is：- 80 DEG C of pre-freezes 4-5h, -45 DEG C of freeze-drying 48h.
Further, in step (2), after freeze-drying, perfluoro-compound is filled with into nanometer microvesicle, the chitosan is made
Nanometer microvesicle.
Further, it is described to be selected from perfluoropropane, hexafluoroethane, sulfur hexafluoride, perfluor containing perfluoro-compound in step (2)
At least one of pentane.
Further, in step (2), after freeze-drying, first nanometer microvesicle is vacuumized, then is filled with entirely into nanometer microvesicle
Fluoride.
Further, in step (2), when being filled with liquid perfluor pentane, vaporization process, it is made and is enclosed with perfluor pentane
Chitosan nano microvesicle.
Further, when being filled with liquid perfluor pentane, the temperature of vaporization process is 40 DEG C -60 DEG C.
Further, in step (1), antineoplastic is first added into reaction solution, after hatching, adds crosslinking agent.
I other words can also prepare medicament-carried nano microvesicle, for targeted therapy, on specific medicine, it can also be that gene target is controlled
Treat medicine etc..
Further, the antineoplastic is selected from Doxorubicin.
Second aspect of the present invention provides a kind of chitosan nano microvesicle, including hollow part, contains chitosan and polyacrylic acid
Shell, filled with air or perfluoro-compound in the hollow part.The chitosan nano microvesicle can be made by preceding method.
Further, the shell includes polypropylene acid layer, chitosan layer successively from inside to outside, and the shell is also containing anti-
Tumour medicine so that microvesicle, which has, carries medicine function.
Third aspect present invention provides above-mentioned chitosan nano microvesicle in ultrasonic development or high intensity focused ultrasound synergy
Purposes.
As described above, chitosan nano microvesicle of the present invention and preparation method thereof and purposes, have the advantages that：This
Chitosan is dissolved in acrylic acid solution by invention by a certain percentage, and adding initiator makes acrylic acid polymerize with chitosan instead
After the hollow Nano grain that inner aqueous should be can obtain, the moisture (or being re-filled with perfluoro-compound) of boring structure, system are removed
After the nanometer microvesicle obtained dissolves again, there is the effect such as ultrasonic development, high intensity focused ultrasound ablation of tissue synergy.
Brief description of the drawings
Fig. 1 is shown as the transmission electron microscope picture of the nanoparticle prepared in embodiment 1.
Fig. 2 (a) is shown as ultrasonic development figure of the chitosan nano microvesicle in water pocket in the embodiment of the present invention 4.
Fig. 2 (b) is shown as ultrasonic development figure of the buffer control in water pocket in the embodiment of the present invention 4.
Fig. 3 (a) is shown as ultrasonic development figure of the nanoparticle in water pocket in the embodiment of the present invention 5.
Fig. 3 (b) is shown as ultrasonic development figure of the buffer control in water pocket in the embodiment of the present invention 5.
Fig. 4 (a) is shown as ultrasonic development figure of the chitosan nano microvesicle in water pocket in the embodiment of the present invention 6.
Fig. 4 (b) is shown as ultrasonic development figure of the buffer control in water pocket in the embodiment of the present invention 6.
Fig. 5 is shown as chitosan nano microbubble structure schematic diagram made from 1-3 of the embodiment of the present invention.
Embodiment
Illustrate embodiments of the present invention below by way of specific instantiation, those skilled in the art can be by this specification
Disclosed content understands other advantages and effect of the present invention easily.The present invention can also pass through specific realities different in addition
The mode of applying is embodied or practiced, the various details in this specification can also be based on different viewpoints with application, without departing from
Various modifications or alterations are carried out under the spirit of the present invention.
In addition, it is to be understood that one or more method and steps mentioned in the present invention do not repel before and after the combination step
There can also be other method step or other method step can also be inserted between the step of these are specifically mentioned, unless separately
It is described；It should also be understood that the combination annexation between one or more equipment/devices mentioned in the present invention is not repelled
Can also have other equipment/device before and after the unit equipment/device or two equipment/devices specifically mentioning at these it
Between can also insert other equipment/device, unless otherwise indicated.Moreover, unless otherwise indicated, the numbering of various method steps is only
Differentiate the convenient tool of various method steps, rather than ordering or the enforceable model of the restriction present invention for limitation various method steps
Enclose, its relativeness is altered or modified, and in the case where changing technology contents without essence, when being also considered as, the present invention is enforceable
Category.
The molecular weight of chitosan is 100kDa-800kDa, deacetylation 60%-100% in following examples.
Embodiment 1
A kind of preparation method of chitosan nano microvesicle, comprises the following steps：
(1) acrylic acid aqueous solution that 50mL mass concentrations are 0.44% is prepared, adds chitosan, the chitosan mass of addition
(unit g) is the 1% of acrylic acid aqueous solution volume (Unit/mL), and stirring is complete to dissolving, and is heated to 75 DEG C under nitrogen protection, adds
Enter 70mg potassium peroxydisulfates, after reacting about 1h, temperature is set as 60 DEG C, continue to react 80min, be then filtered to remove product miscellaneous
Matter, it is subsequently poured into bag filter, then is placed in pH=4.5 Acetic acid-sodium acetate buffer solution, magnetic agitation 24h, dialysis removes impurity elimination
Taken out after matter, add 0.078g glutaraldehydes, be heated to 40 DEG C and react, after 2h, reaction finishes, and nanoparticle suspension, nanometer is made
Grain particle diameter is about 100nm, and has hollow-core construction, and its form of transmission electron microscope observing, transmission electron microscope picture is shown in Fig. 1, it is seen that nanometer
Grain form rounding, particle diameter is about 100nm.
(2) mannitol is added into nanoparticle suspension made from step (1), (unit g) is nanometer to the quality of mannitol
The 5% of grain suspension volume (Unit/mL), in -80 DEG C of pre-freeze 4h, takes out from the equipment of -80 DEG C of pre-freezes, and it is dry to place into freezing
In dry machine, -45 DEG C of freeze-drying 48h, take out, be placed in glass tubule, with vacuum pumping negative pressure, adding appropriate liquid perfluor just
Pentane, produced after vaporizing 30min in 40 DEG C.
Embodiment 2
A kind of preparation method of chitosan nano microvesicle, comprises the following steps：
(1) acrylic acid solution that 50mL mass concentrations are 0.22% is prepared, adds chitosan, the chitosan mass of addition
(unit g) is the 0.5% of acrylic acid aqueous solution volume (Unit/mL), and stirring is complete to dissolving, and 75 DEG C are heated under nitrogen protection,
45mg potassium peroxydisulfates are added, after reacting about 1h, temperature is set as 60 DEG C, after 100min, product is filtered to remove impurity, then
Bag filter is poured into, then is placed in pH=4.5 Acetic acid-sodium acetate buffer solution, is taken out after magnetic agitation 24h, adds 0.035g penta
Dialdehyde, it is heated to 40 DEG C and reacts, after 2h, reaction finishes, and nanoparticle suspension is made, and nanoparticle particle diameter is about 100nm and had
Hollow-core construction, its structural form are similar with Fig. 1.
(2) lactose is added into nanoparticle suspension made from step (1), (unit g) is mixed the quality of lactose for nanoparticle
The 5% of suspension volume (Unit/mL), in -80 DEG C of pre-freeze 5h, taken out from the equipment of -80 DEG C of pre-freezes, place into freeze drier
In, -45 DEG C of freeze-drying 48h, take out, be placed in glass tubule, with vacuum pumping negative pressure, be filled with perfluoropropane gas, replace
After three times, the chitosan nano microvesicle for being enclosed with perfluoropropane gas is made.
Embodiment 3
A kind of preparation method of chitosan nano microvesicle, comprises the following steps：
(1) acrylic acid solution that 50mL mass concentrations are 0.44% is prepared, adds chitosan, the chitosan mass of addition
(unit g) is the 1% of acrylic acid aqueous solution volume (Unit/mL), is heated to 70 DEG C under nitrogen protection, adds 60mg potassium peroxydisulfates,
After reacting about 1h, temperature is set as 60 DEG C, product is filtered to remove impurity after 80min, then pour into bag filter, be placed in pH=
In 4.5 Acetic acid-sodium acetate buffer solution, taken out after magnetic agitation 24h, add 0.078g glutaraldehydes, be heated to 40 DEG C of reactions, 2h
Afterwards, reaction finishes, and nanoparticle suspension is made, and nanoparticle particle diameter is about 100nm and has hollow-core construction, its structural form and figure
1 is similar.
(2) mannitol is added into nanoparticle suspension made from step (1), (unit g) is nanometer to the quality of mannitol
The 5% of grain suspension volume (Unit/mL), in -80 DEG C of pre-freeze 5h, takes out from the equipment of -80 DEG C of pre-freezes, and it is dry to place into freezing
In dry machine, -45 DEG C of freeze-drying 48h, take out, be not re-filled with fluorocarbon gas, the nanoparticle boring obtained after freeze-drying
The moisture content in region is removed, and its inside is filled with air, and the nanometer microvesicle for being enclosed with air possesses certain ultrasonic development function.
Embodiment 4
Development evaluation is carried out to chitosan nano microvesicle made from embodiment 1
Using Bai Sheng diasonograph MYLAB90, ultrasonic probe is superficial probe LA523, supersonic frequency 5.0-
10MHz.The nanometer microvesicle being prepared successively by step (1) and step (2) in above-described embodiment 1 is fitted into water pocket, then will
Water pocket, which is put into the container for fill de aerated water, carries out development experiment, will separately fill the degassing of pH4.5 Acetic acid-sodium acetate buffer solution
Water pocket is as control, the development situation of two water pockets of paired observation.
Fig. 2 (a) is shown as ultrasonic development figure of the nanometer microvesicle in water pocket, Fig. 2 (b) in the present embodiment and is shown as this implementation
Ultrasonic development figure of the buffer control in water pocket in example, from result, prepared by embodiment 1 is loaded with receiving for perfluor pentane
There is rice microvesicle stronger development to act on, and the Acetic acid-sodium acetate buffer solution water pocket control groups of pH 4.5 under ultrasonic development only
It can be seen that the cyst wall curve of water pocket, shows that nanometer microvesicle prepared by embodiment 1 possesses developing function.
The nanometer microvesicle that embodiment 2 obtains also has the developing function for being similar to nanometer microvesicle in embodiment 1.
Embodiment 5
Ultrasonic development evaluation is carried out to the nano-particle of hollow-core construction made from step (1) in embodiment 3
Use DP3300 diasonographs, supersonic frequency 6.5MHz.Nanometer prepared by step (1) in above-described embodiment 3
Grain is fitted into water pocket, then water pocket is put into the container for fill de aerated water and carries out development experiment, will separately fill the vinegar that pH is 4.5
The degassing water pocket of acid-sodium-acetate buffer is as control, the development situation of two water pockets of paired observation.
Fig. 3 (a) is shown as ultrasonic development figure of the nanoparticle in water pocket, Fig. 3 (b) in the present embodiment and is shown as the present embodiment
Ultrasonic development figure of the middle buffer control in water pocket, the nano-particle and buffer solution water pocket prepared from result, step (1)
Cyst wall curve to impinging upon only visible water pocket under ultrasonic development, show that nano-particle prepared by step (1) does not possess development work(
Energy.
Embodiment 6
Ultrasonic development evaluation is carried out to chitosan nano microvesicle made from step (2) in embodiment 3
Use DP3300 diasonographs, supersonic frequency 6.5MHz.Weigh in appropriate embodiment 3 and received made from step (2)
Rice microvesicle, is dissolved in pH4.5 buffer solutions, is contained after dissolving in water pocket, then water pocket is put into the container for filling de aerated water again
In carry out development experiment, will separately fill the degassing water pockets of PH4.5 buffer solutions as control, the development feelings of two water pockets of paired observation
Condition.
Fig. 4 (a) is shown as ultrasonic development figure of the chitosan nano microvesicle in water pocket, Fig. 4 (b) in the present embodiment and is shown as
Ultrasonic development figure of the buffer control in water pocket in the present embodiment, from result, chitosan nano microvesicle water pocket have compared with
Strong development effect, and buffer solution water pocket is to impinging upon the cyst wall curve of only visible water pocket under ultrasonic development, show using implementing
Microvesicle made from air, which is filled with, in the step of example 3 (2) possesses developing function.
Found from the experimental result of embodiment 5 and embodiment 6, nanoparticle suspension just possesses after having to pass through freeze-drying
Ultrasonic development function.
Experiment finds that the nanometer microvesicle for not adding freeze drying protectant and being freezed to obtain is difficult to redissolve, and this nanometer micro-
After bubble plus buffer solution, there is flocculent deposit and suspension, it is impossible to be used in injection, therefore, freeze drying protectant conform to for preparation
The nanometer microvesicle asked is most important.
Embodiment 7
The high intensity focused ultrasound synergistic function evaluation of chitosan nano microvesicle
Fresh in vitro beef liver is carried out to cut into the cube block that size is 10cm*6cm*6cm, degassing drum is positioned over, takes off
Bottom is placed on after gas 40min to be had in the plastic containers of entrant sound film, and container bottom is immersed in de aerated water.Pass through ultrasound observation
The less region of tube chamber in beef liver tissue, and beef liver is divided into 3-4 aspect, each 2-3 point of aspect, between each aspect,
Interval is all higher than 1cm between each point, then by nanometer microbubble solution that 0.5mL concentration is 0.068g/mL (microbubble solution by
A nanometer microvesicle made from embodiment 3 is dissolved in Acetic acid-sodium acetate buffer solution and formed) in inhalation syringe, avoid cavity portion in beef liver
Position, syringe needle is vertically pierced into beef liver middle part, depth substantially 2cm, by ultrasonic contrast locating injection position, then by nanometer
Microvesicle injects, syringe needle of quickly choosing, and starts HIFU (high intensity focused ultrasound) immediately and is irradiated, irradiation power P=120W, spoke
Be 5s according to the time, after beef liver is taken out, by cut into slices find area for treatment, take pictures, calculate the damaged area of irradiation zone
Size, experiment is repeated 3 times, experimental result is counted；The beef liver tissue for not injecting microvesicle is directly separately subjected to HIFU ablations
Treatment is tested as a comparison.Test result indicates that beef liver tissue damage area average out to after chitosan nano microvesicle is injected
36.9mm2, beef liver tissue damage area average out to 8.5mm after chitosan nano microvesicle is not injected2, show shell prepared by the present invention
Glycan nanometer microvesicle can dramatically increase the damaged area of HIFU Treatment.
Embodiment 8
A kind of preparation for carrying medicine chitosan nano microvesicle, comprises the following steps：
(1) acrylic acid solution that 50mL mass concentrations are 0.44% is prepared, adds chitosan, the chitosan mass of addition
(unit g) is the 1% of acrylic acid aqueous solution volume (Unit/mL), is heated to 75 DEG C under nitrogen protection, adds 30mg potassium peroxydisulfates,
After reacting about 1h, temperature is set as 65 DEG C, product is filtered to remove impurity after 60min, then pour into bag filter, be placed in pH=
In 4.5 Acetic acid-sodium acetate buffer solution, taken out after magnetic agitation 24h.
(2) 0.5g Doxorubicins are added in reaction solution, after 40 DEG C hatch 48h, 0.078g glutaraldehydes is added, is heated to 40 DEG C
React, after 2h, reaction finishes, and nanoparticle suspension is made, and nanoparticle particle diameter is about 100nm and has hollow-core construction, its structure
Form is similar with Fig. 1.
(3) mannitol is added into nanoparticle suspension made from step (2), (unit g) is nanometer to the quality of mannitol
The 5% of grain suspension volume (Unit/mL), in -80 DEG C of pre-freeze 5h, in being taken from the equipment of -80 DEG C of pre-freezes, it is dry to place into freezing
In dry machine, -45 DEG C of freeze-drying 48h, take out, with vacuum pumping negative pressure, add appropriate liquid perfluor pentane, in 40 DEG C of vaporizations
The nanometer microvesicle of medicine dual-use function is obtained with development and carried after 30min.
Chitosan nano microbubble structure schematic diagram made from embodiment 1-3, including hollow part 1, shell 2 are illustrated in figure 5,
Filled with air or perfluoro-compound in hollow part 1, shell 2 made from embodiment 1-3 contains chitosan and polyacrylic acid, shell 2 from
Polypropylene acid layer 21, chitosan layer 22 are followed successively by from inside to outside, and shell 2 made from embodiment 8 not only contains chitosan and polypropylene
Acid, also containing antineoplastic Doxorubicin, therefore, chitosan nano microvesicle, which also has, carries medicine function.The external diameter of shell 2 is about
100nm。
The above-described embodiments merely illustrate the principles and effects of the present invention, not for the limitation present invention.It is any ripe
Know the personage of this technology all can carry out modifications and changes under the spirit and scope without prejudice to the present invention to above-described embodiment.Cause
This, those of ordinary skill in the art is complete without departing from disclosed spirit and institute under technological thought such as
Into all equivalent modifications or change, should by the present invention claim be covered.
Claims (10)
1. a kind of preparation method of chitosan nano microvesicle, it is characterised in that comprise the following steps：
(1) acrylic acid aqueous solution is prepared, chitosan is added, after dissolving, solution is heated to 60-80 in the presence of protective gas
DEG C, initiator for reaction 10min-60min is added, solution temperature is then reduced to 50 DEG C -65 DEG C, reacts 60-120min, reaction
After end, filter and dialyse and remove impurity, after processing terminates, add crosslinking agent, heating, react and nanoparticle suspension is made；
(2) freeze drying protectant is added into the nanoparticle suspension, be freeze-dried, the chitosan nano microvesicle is made.
2. preparation method according to claim 1, it is characterised in that：In step (1), by solution temperature be reduced to 50 DEG C-
65 DEG C, react 80-100min；
And/or in step (1), the mass concentration of the acrylic acid aqueous solution is 0.1-1.6%, preferably 0.22-0.44%；
And/or in step (1), the chitosan mass (g) of addition is the 0.5-3.5% of acrylic acid aqueous solution volume (mL), preferably
For 0.5-1%；
And/or in step (1), the molecular weight of the chitosan is 100kDa-800kDa, deacetylation 60%-100%.
3. preparation method according to claim 1, it is characterised in that：The initiator is selected from potassium peroxydisulfate；
And/or the addition of the initiator is 30-70mg, the volume of acrylic acid aqueous solution is 50mL.
4. preparation method according to claim 1, it is characterised in that：In step (1), the protective gas be selected from nitrogen or
Other inert gases；
And/or in step (1), solution is heated to 70-75 DEG C in the presence of protective gas；
And/or in step (1), the crosslinking agent is selected from least one of glutaraldehyde, sodium tripolyphosphate；
And/or in step (1), add crosslinking agent quality (unit g) be reaction solution volume (Unit/mL) 0.05-2%；
And/or in step (1), after adding crosslinking agent, solution is heated to 40 DEG C of -50 DEG C of reactions；
And/or in step (1), after adding crosslinking agent, reaction time 1h-2h.
5. preparation method according to claim 1, it is characterised in that：In step (2), the freeze drying protectant is selected from sweet dew
At least one of alcohol, glucose, sucrose, lactose；
And/or in step (2), adding the quality of freeze drying protectant, (unit g) is nanoparticle suspension volume (Unit/mL)
1%-10%, preferably 1-5%；
And/or in step (2), freeze-drying method is：- 80 DEG C of pre-freezes 4-5h, -45 DEG C of freeze-drying 24-48h；
And/or in step (2), after freeze-drying, perfluoro-compound is filled with into nanometer microvesicle, it is micro- that the chitosan nano is made
Bubble, the perfluoro-compound are selected from least one of perfluoropropane, hexafluoroethane, sulfur hexafluoride, perfluor pentane.
6. preparation method according to claim 1, it is characterised in that：In step (1), first added into reaction solution antitumor
Medicine, after hatching, add crosslinking agent.
7. preparation method according to claim 6, it is characterised in that：The antineoplastic is selected from Doxorubicin.
A kind of 8. chitosan nano microvesicle, it is characterised in that：It is outer including hollow part (1) and containing chitosan and polyacrylic acid
Shell (2), the hollow part (1) are interior filled with air or perfluoro-compound.
9. chitosan nano microvesicle according to claim 8, it is characterised in that the shell (2) is wrapped successively from inside to outside
Polypropylene acid layer (21), chitosan layer (22) are included, the shell (2) also contains antineoplastic.
10. chitosan nano microvesicle according to claim 8 or claim 9 is in ultrasonic development or high intensity focused ultrasound synergy
Purposes.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108836392A (en) * | 2018-03-30 | 2018-11-20 | 中国科学院深圳先进技术研究院 | Ultrasonic imaging method, device, equipment and storage medium based on ultrasonic RF signal |
| CN109223731A (en) * | 2018-10-30 | 2019-01-18 | 贵阳中医学院第附属医院 | A kind of gastrointestinal tract is stable and targets the hollow nanospheres and preparation method thereof of enteric microorganism aggregation |
| CN111067872A (en) * | 2019-12-30 | 2020-04-28 | 浙江大学 | Polydopamine nanoparticle stable microbubble dispersion system for efficient intravenous oxygen supply and preparation method thereof |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1314430A (en) * | 2001-04-26 | 2001-09-26 | 南京大学 | Nanometer microball of chitosan-polyacrylic acid composite and its producing method and use |
| CN101721718A (en) * | 2008-10-28 | 2010-06-09 | 鲁翠涛 | Lipid microbubble and preparation method thereof |
| CN102391428A (en) * | 2011-07-18 | 2012-03-28 | 河北科技师范学院 | Preparation method for maleoyl chitosan polymer microspheres |
| CN102552157A (en) * | 2010-12-17 | 2012-07-11 | 南京大学 | Chitosan-polyacrylic acid composite nanometer micro-sphere covered with precious metal on surface as well as preparation method and application thereof |
| CN104069517A (en) * | 2014-07-16 | 2014-10-01 | 复旦大学 | Magnetism polymer nanometer microsphere material capable of degrading ring structure, as well as preparation method and application of magnetism polymer nanometer microsphere material |
| CN104524602A (en) * | 2014-12-26 | 2015-04-22 | 宁波市第一医院 | Folate receptor targeting ultrasound contrast nanobubble and preparation method thereof |
| CN106075471A (en) * | 2016-07-12 | 2016-11-09 | 上海纳米技术及应用国家工程研究中心有限公司 | A kind of have lympha targeted fluorocarbon emulsion and preparation method thereof |
| CN106620720A (en) * | 2015-07-31 | 2017-05-10 | 温州生物材料与工程研究所 | Modification of carrier gas microbubbles having different charges respectively through step-by-step method and one-step method, and preparation of microspheres through one-step method |
| US20170136144A1 (en) * | 2015-11-12 | 2017-05-18 | John C. Herr | Compositions and methods for vas-occlusive contraception |
-
2017
- 2017-08-29 CN CN201710756784.4A patent/CN107537044B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1314430A (en) * | 2001-04-26 | 2001-09-26 | 南京大学 | Nanometer microball of chitosan-polyacrylic acid composite and its producing method and use |
| CN101721718A (en) * | 2008-10-28 | 2010-06-09 | 鲁翠涛 | Lipid microbubble and preparation method thereof |
| CN102552157A (en) * | 2010-12-17 | 2012-07-11 | 南京大学 | Chitosan-polyacrylic acid composite nanometer micro-sphere covered with precious metal on surface as well as preparation method and application thereof |
| CN102391428A (en) * | 2011-07-18 | 2012-03-28 | 河北科技师范学院 | Preparation method for maleoyl chitosan polymer microspheres |
| CN104069517A (en) * | 2014-07-16 | 2014-10-01 | 复旦大学 | Magnetism polymer nanometer microsphere material capable of degrading ring structure, as well as preparation method and application of magnetism polymer nanometer microsphere material |
| CN104524602A (en) * | 2014-12-26 | 2015-04-22 | 宁波市第一医院 | Folate receptor targeting ultrasound contrast nanobubble and preparation method thereof |
| CN106620720A (en) * | 2015-07-31 | 2017-05-10 | 温州生物材料与工程研究所 | Modification of carrier gas microbubbles having different charges respectively through step-by-step method and one-step method, and preparation of microspheres through one-step method |
| US20170136144A1 (en) * | 2015-11-12 | 2017-05-18 | John C. Herr | Compositions and methods for vas-occlusive contraception |
| CN106075471A (en) * | 2016-07-12 | 2016-11-09 | 上海纳米技术及应用国家工程研究中心有限公司 | A kind of have lympha targeted fluorocarbon emulsion and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| YONG HU ET AL: "Hollow Chitosan/Poly(acrylic acid) Nanospheres as Drug Carriers", 《BIOMACROMOLECULES》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108836392A (en) * | 2018-03-30 | 2018-11-20 | 中国科学院深圳先进技术研究院 | Ultrasonic imaging method, device, equipment and storage medium based on ultrasonic RF signal |
| CN108836392B (en) * | 2018-03-30 | 2021-06-22 | 中国科学院深圳先进技术研究院 | Ultrasonic imaging method, device and equipment based on ultrasonic RF signal and storage medium |
| CN109223731A (en) * | 2018-10-30 | 2019-01-18 | 贵阳中医学院第附属医院 | A kind of gastrointestinal tract is stable and targets the hollow nanospheres and preparation method thereof of enteric microorganism aggregation |
| CN111067872A (en) * | 2019-12-30 | 2020-04-28 | 浙江大学 | Polydopamine nanoparticle stable microbubble dispersion system for efficient intravenous oxygen supply and preparation method thereof |
| WO2021135682A1 (en) * | 2019-12-30 | 2021-07-08 | 浙江大学 | Microbubble dispersion system used for efficiently supplying oxygen to veins and stabilized by polydopamine nanoparticles and preparation method for microbubble dispersion system |
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