CN107515287A - A kind of degradable soluble organic nitrogen assay method of saprobia based on dynamic cultivation - Google Patents
A kind of degradable soluble organic nitrogen assay method of saprobia based on dynamic cultivation Download PDFInfo
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- 125000001477 organic nitrogen group Chemical group 0.000 title claims abstract description 19
- 238000003556 assay Methods 0.000 title claims abstract description 12
- 239000010865 sewage Substances 0.000 claims abstract description 92
- 238000012545 processing Methods 0.000 claims abstract description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 25
- 229910052799 carbon Inorganic materials 0.000 claims description 25
- 238000000034 method Methods 0.000 claims description 21
- 229910052757 nitrogen Inorganic materials 0.000 claims description 18
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 claims description 11
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 claims description 11
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 11
- 239000008367 deionised water Substances 0.000 claims description 10
- 229910021641 deionized water Inorganic materials 0.000 claims description 10
- 239000000945 filler Substances 0.000 claims description 9
- 150000002500 ions Chemical class 0.000 claims description 9
- 238000004587 chromatography analysis Methods 0.000 claims description 7
- 238000002798 spectrophotometry method Methods 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 6
- 238000005375 photometry Methods 0.000 claims description 6
- LNGHGZJAEPDKFE-UHFFFAOYSA-N chloro 2-hydroxybenzoate Chemical compound OC1=CC=CC=C1C(=O)OCl LNGHGZJAEPDKFE-UHFFFAOYSA-N 0.000 claims description 5
- 238000005374 membrane filtration Methods 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 125000001637 1-naphthyl group Chemical group [H]C1=C([H])C([H])=C2C(*)=C([H])C([H])=C([H])C2=C1[H] 0.000 claims description 4
- 229920006221 acetate fiber Polymers 0.000 claims description 4
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 claims description 4
- 239000010802 sludge Substances 0.000 claims description 4
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 238000004611 spectroscopical analysis Methods 0.000 claims description 2
- 239000010841 municipal wastewater Substances 0.000 abstract description 5
- 230000003068 static effect Effects 0.000 description 6
- 238000011081 inoculation Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 230000010076 replication Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/1826—Organic contamination in water
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses a kind of degradable soluble organic nitrogen assay method of saprobia based on dynamic cultivation, belong to municipal wastewater processing technology field.Assay method comprises the following steps：1) sewage sample to be measured is pre-processed；2) it is aerated；3) pre-flush filter column；4) cultivate；5) sewage sample BDON to be measured is calculated.Assay method provided by the invention is easy to operate, minute is short, can be widely applied to municipal wastewater BDON measure.
Description
Technical field
The invention belongs to municipal wastewater processing technology field, more specifically to a kind of sewage based on dynamic cultivation
Biodegradable soluble organic nitrogen assay method.
Background technology
Dissolution of contaminated water total nitrogen includes inorganic nitrogen (ammonia nitrogen, nitrate nitrogen and nitrite nitrogen) and soluble organic nitrogen
(dissolved organic nitrogen,DON).Most of inorganic nitrogen passes through nitrification-anti-in biochemical processing procedure of sewage
Nitrification is removed.By contrast, DON is difficult to be removed in sewage disposal, causes DON in water outlet to account for total nitrogen ratio liter
It is high.In low total nitrogen water outlet, DON accounts for the ratio of total nitrogen up to 25%~80%.DON can cause fouling membrane and the nitrogenous class of generation to disappear
The problems such as toxic byproduct, influence the processing of sewage plant successive depths.Biodegradable soluble organic nitrogen (biodegradable
Dissolved organic nitrogen, BDON) refer to the part DON that can be metabolized and be utilized by heterotroph in sewage, it can use
To predict and weigh removal efficiency of the Sewage Biological Treatment unit to DON.
Domestic and foreign scholars mainly determine BDON by static culture method at present.First water sample to be measured is removed by membrane filtration
Insoluble substance, using bacterium as the inoculation of inoculation bacterium after lucifuge culture 20~28 days at 20 DEG C~25 DEG C, cultivate front and rear DON
Difference is BDON.This method testing cost is low, while is determined available for more water samples batch, but due to needing 20~28 days
Incubation time, sewage plant staff can not obtain sewage plant sewage BDON change in time.In addition, experimenter generally requires
Multiple preliminary experiment determines final inoculation liquid concentration, and operating procedure is time-consuming and cumbersome.Therefore, reality of the assay method in sewage plant
It is restricted in the application of border.Routine monitoring for sewage treatment plant sewage BDON is, it is necessary to a kind of quick and precisely and easy to operate
Assay method.
The content of the invention
1st, to solve the problems, such as
The invention aims to solve biodegradable organic nitrogen method in existing static culture method measure sewage to take
And the problem of cumbersome, and the present invention provides a kind of degradable soluble organic nitrogen assay method of saprobia based on dynamic cultivation
It is easy to operate, minute is short, can be widely applied to municipal wastewater BDON measure.
2nd, technical scheme
To solve the above problems, the present invention adopts the following technical scheme that.
A kind of degradable soluble organic nitrogen assay method of saprobia based on dynamic cultivation, its step are：
(1) sewage sample to be measured is pre-processed：With after membrane filtration sewage sample to be measured determine dissolved organic carbon concentration,
And sewage sample to be measured is handled according to the concentration of the dissolved organic carbon of measure；
(2) it is aerated：Sewage sample to be measured after step (1) processing is persistently aerated；
(3) pre-flush filter column：First use deionized water rinsing filter column, then with the sewage to be measured after step (2) Air Exposure
Sample wash filter column；
(4) cultivate：Sewage sample to be measured after step (2) Air Exposure is flowed through into filter column, determines filter column disengaging respectively
Water DON concentration, is designated as DON respectivelyinfAnd DONeff；
(5) sewage sample BDON to be measured is calculated.
Preferably, the filter membrane described in step (1) is acetate fiber filter membrane, and aperture is 0.22 μm.
Preferably, in step (1), as dissolved organic carbon concentration≤30mg/L of sewage sample to be measured, sewage to be measured
Sample is without dilution；As the dissolved organic carbon concentration ＞ 30mg/L of sewage sample to be measured, with deionized water by sewage to be measured
Sample is diluted to its dissolved organic carbon concentration between 15mg/L~25mg/L.
Preferably, the concentration of the dissolved organic carbon described in step (1) is determined using dry method total organic carbon analyzer.
Preferably, in step (3), deionized water volume is 2~3 column volumes, and flow velocity is 20mL/min~40mL/min；
Sewage sample volume to be measured is 2~4 column volumes, and flow velocity is 20mL/min~40mL/min.
Preferably, the filler of the filter column described in step (4) is haydite, and particle diameter is 4mm~6mm；Wherein filler inoculation is dirty
Mud is from sewage plant where sewage sample to be measured.
Preferably, the flow velocity that sewage sample to be measured flows through filter column in step (4) is 2mL/min~5mL/min.
Preferably, the DON concentration described in step (4) passes through total dissolubility nitrogen and ammonia nitrogen, nitrate nitrogen and nitrite nitrogen
Mathematic interpolation and obtain.
Preferably, the concentration of total dissolubility nitrogen is using potassium persulfate oxidation-chromatography of ions or potassium persulfate oxidation-ultraviolet
Spectrophotometry；The concentration of ammonia nitrogen is using salicylic acid-hypochlorite photometry measure；The concentration of nitrate nitrogen uses ion color
Spectrometry or determined by ultraviolet spectrophotometry；The concentration of nitrite nitrogen uses the chromatography of ions or N- (1- naphthyls)-ethylenediamine luminosity
Method determines.
Preferably, the BDON calculation formula described in step (5) are：BDON=DONinf- DONeff。
3rd, beneficial effect
Compared to prior art, beneficial effects of the present invention are：
(1) short, the incubation time (including pre-flush time) of BDON assay methods required time in sewage provided by the invention
＜ 8 hours, and traditional static method measure sewage BDON needs 20~28 days；
(2) present invention is simple to operate, finding speed is fast, has the characteristics of being easy to continuously run, can be widely applied to municipal administration
Sewage BDON is determined.
Brief description of the drawings
Fig. 1 is the BDON concentration of the sewage sample 1-3 that embodiment 1-3 is determined respectively in the present invention；
Fig. 2 is the BDON concentration of the sewage sample 1-3 based on traditional static cultivation measure.
Embodiment
The present invention will be described in detail below in conjunction with the accompanying drawings.
Embodiment 1
(1) Nanjing municipal sewage plant water outlet (being designated as sewage sample 1) is taken, with the acetate fiber that aperture is 0.22 μm
Membrane filtration sewage sample 1；The dissolved organic carbon of sewage sample 1 is dense after being filtered using dry method total organic carbon analyzer measure
Degree, it is 22mg/L；Due to the concentration ＜ 30mg/L of dissolved organic carbon, sewage sample is without dilution；
(2) sewage sample handled through step (1) is persistently aerated；
(3) filter column, flow velocity 20mL/min first are got express developed with the deionized water of 2 column volumes；Again with 3 column volumes
The sewage sample handled through step (2) gets filter column express developed；Flow velocity is 30mL/min；Filter column is cylindrical, high 40cm, internal diameter
4.5cm；Bed stuffing height 30cm, filler are haydite, and particle diameter is 4mm~6mm；Filler seed sludge is where sewage sample 1
Sewage plant aerobic section；
(4) the 300mL sewage samples handled through step (2) are flowed through into filter column, flow velocity 2mL/min；Over cure is respectively adopted
Sour potassium oxidation-chromatography of ions, salicylic acid-hypochlorite photometry, the chromatography of ions and ion-chromatographic determination sewage sample 1
Total dissolubility nitrogen in Inlet and outlet water, ammonia nitrogen, the concentration of nitrate nitrogen and nitrite nitrogen；By total dissolubility nitrogen and ammonia nitrogen, nitrate nitrogen and
The mathematic interpolation filter column Inlet and outlet water DON concentration of nitrite nitrogen, is designated as DON respectivelyinfAnd DONeff；
(5) by BDON calculation formula：BDON=DONinf- DONeff, the BDON for obtaining sewage sample 1 is 0.61mg/L.
In order to improve the accuracy of measure and reliability, the present embodiment replication is averaged three times, as a result such as Fig. 1
It is shown.
Embodiment 2
(1) Nanjing municipal sewage plant water inlet (being designated as sewage sample 2) is taken, with the acetate fiber that aperture is 0.22 μm
Membrane filtration sewage sample；The dissolved organic carbon of sewage sample 2 is dense after being filtered using dry method total organic carbon analyzer measure
Degree, it is 75mg/L；Dissolved organic carbon ＞ 30mg/L, sewage sample are diluted to its dissolved organic carbon with deionized water and are
20mg/L；
(2) sewage sample handled through step (1) is persistently aerated；
(3) filter column, flow velocity 40mL/min first are got express developed with the deionized water of 3 column volumes；Again with 2 column volumes
The sewage sample handled through step (2) gets filter column, flow velocity 20mL/min express developed；Filter column is cylindrical, high 40cm, internal diameter
4.5cm；Bed stuffing height 30cm, filler are haydite, and particle diameter is 4mm~6mm；Filler seed sludge is where sewage sample 2
Sewage plant aerobic section；
(4) the 600mL sewage samples handled through step (2) are flowed through into filter column, flow velocity 5mL/min；Over cure is respectively adopted
Sour potassium oxidation-chromatography of ions, salicylic acid-hypochlorite photometry, ultraviolet spectrophotometry and N- (1- naphthyls)-ethylenediamine light
Degree method determines the concentration of total dissolubility nitrogen in the Inlet and outlet water of sewage sample 2, ammonia nitrogen, nitrate nitrogen and nitrite nitrogen；Pass through total dissolubility
The mathematic interpolation filter column Inlet and outlet water DON concentration of nitrogen and ammonia nitrogen, nitrate nitrogen and nitrite nitrogen, is designated as DON respectivelyinfAnd DONeff；
(5) by BDON calculation formula：BDON=DONinf- DONeff, the BDON for obtaining sewage sample 2 is 5.90mg/L.
In order to improve the accuracy of measure and reliability, the present embodiment replication is averaged three times, as a result such as Fig. 1
It is shown.
Embodiment 3
(1) Wuxi municipal sewage plant aerobic section sewage (being designated as sewage sample 3) is taken, with the vinegar that aperture is 0.22 μm
Sour fibrous filter membrane filter sewage sample；Dissolubility using sewage sample 3 after the measure filtering of dry method total organic carbon analyzer is organic
Concentration of carbon, it is 44mg/L；Dissolved organic carbon is more than 30mg/L, and it is organic that sewage sample with deionized water is diluted to its dissolubility
Carbon is 25mg/L；
(2) sewage sample handled through step (1) is persistently aerated；
(3) filter column, flow velocity 30mL/min first are got express developed with the deionized water of 2 column volumes；Again with 2 column volumes
The sewage sample handled through step (2) gets filter column, flow velocity 30mL/min express developed；Filter column is cylindrical, high 40cm, internal diameter
4.5cm；Bed stuffing height 30cm, filler are haydite, and particle diameter is 4mm~6mm；Filler seed sludge is where sewage sample 3
Sewage plant aerobic section；
(4) the 400mL sewage samples handled through step (2) are flowed through into filter column, flow velocity 3mL/min；Over cure is respectively adopted
Sour potassium oxidation-ultraviolet spectrophotometry, salicylic acid-hypochlorite photometry, ultraviolet spectrophotometry and N- (1- naphthyls)-second two
Amine photometry determines the concentration of total dissolubility nitrogen in the Inlet and outlet water of sewage sample 3, ammonia nitrogen, nitrate nitrogen and nitrite nitrogen；By total molten
Solution property nitrogen and ammonia nitrogen, the mathematic interpolation filter column Inlet and outlet water DON concentration of nitrate nitrogen and nitrite nitrogen, are designated as DON respectivelyinfWith
DONeff；
(5) by BDON calculation formula：BDON=DONinf- DONeff, the BDON for obtaining sewage sample 3 is 1.40mg/L.
In order to improve the accuracy of measure and reliability, the present embodiment replication is averaged three times, as a result such as Fig. 1
It is shown.
Comparative example
As shown in Fig. 2 the BDON concentration of the sewage sample 1-3 by traditional static cultivation measure be respectively 0.55 ±
0.09mg/L, 5.67 ± 1.00mg/L and 1.33 ± 0.21mg/L.
In summary, as it can be seen from table 1 the BDON concentration for the sewage sample 1-3 that the present invention determines is quiet with being based on tradition
The T test values of the sewage sample 1-3 of state cultivation measure BDON concentration are all higher than 0.05, show two groups of data no significant differences
Property.The present invention is simple to operate, short without preliminary experiment, minute, therefore, can be widely applied to municipal wastewater BDON measure.Table 1
The BDON concentration for the sewage sample 1-3 that the present invention determines is with the sewage sample 1-3's based on traditional static cultivation measure
The T test values of BDON concentration
| Sample 1 | Sample 2 | Sample 3 | |
| T test values | 0.423 | 0.837 | 0.667 |
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.
Claims (10)
1. a kind of degradable soluble organic nitrogen assay method of saprobia based on dynamic cultivation, its step are：
(1) sewage sample to be measured is pre-processed：With the concentration that dissolved organic carbon is determined after membrane filtration sewage sample to be measured, and root
The concentration of dissolved organic carbon according to surveying and determination is handled sewage sample to be measured；
(2) it is aerated：Sewage sample to be measured after step (1) processing is persistently aerated；
(3) pre-flush filter column：First use deionized water rinsing filter column, then with the sewage sample to be measured after step (2) Air Exposure
Rinse filter column；
(4) cultivate：Sewage sample to be measured after step (2) Air Exposure is flowed through into filter column, determines filter column Inlet and outlet water DON respectively
Concentration, DON is designated as respectivelyinfAnd DONeff；
(5) sewage sample BDON to be measured is calculated.
2. according to the degradable soluble organic nitrogen measure side of a kind of saprobia based on dynamic cultivation described in claim 1
Method, it is characterised in that：Filter membrane described in step (1) is acetate fiber filter membrane, and aperture is 0.22 μm.
3. according to the degradable soluble organic nitrogen measure side of a kind of saprobia based on dynamic cultivation described in claim 1
Method, it is characterised in that：It is described as dissolved organic carbon concentration≤30mg/L of described sewage sample to be measured in step (1)
Sewage sample to be measured without dilution；As the dissolved organic carbon concentration ＞ 30mg/L of described sewage sample to be measured, spend
Described sewage sample to be measured is diluted to its dissolved organic carbon concentration between 15mg/L~25mg/L by described ionized water.
4. according to the degradable soluble organic nitrogen measure side of a kind of saprobia based on dynamic cultivation described in claim 2
Method, it is characterised in that：The concentration of dissolved organic carbon described in step (1) is determined using dry method total organic carbon analyzer.
5. according to the degradable soluble organic nitrogen measure side of a kind of saprobia based on dynamic cultivation described in claim 1
Method, it is characterised in that：In step (3), described deionized water volume is 2~3 column volumes, flow velocity be 20mL/min~
40mL/min；Described sewage sample volume to be measured is 2~4 column volumes, and flow velocity is 20mL/min~40mL/min.
6. had according to the degradable dissolubility of a kind of saprobia based on dynamic cultivation described in claim 1-5 any one
Machine nitrogen determination method, it is characterised in that：The filler of filter column described in step (4) is haydite, and particle diameter is 4mm~6mm；Wherein fill out
Expect seed sludge from sewage plant where sewage sample to be measured.
7. had according to the degradable dissolubility of a kind of saprobia based on dynamic cultivation described in claim 1-5 any one
Machine nitrogen determination method, it is characterised in that：The flow velocity that sewage sample to be measured flows through filter column in step (4) is 2mL/min~5mL/
min。
8. according to the degradable soluble organic nitrogen measure side of a kind of saprobia based on dynamic cultivation described in claim 1
Method, it is characterised in that：The difference that DON concentration described in step (4) passes through total dissolubility nitrogen and ammonia nitrogen, nitrate nitrogen and nitrite nitrogen
Value is calculated and obtained.
9. according to the degradable soluble organic nitrogen measure side of a kind of saprobia based on dynamic cultivation described in claim 8
Method, it is characterised in that：The concentration of total dissolubility nitrogen is using potassium persulfate oxidation-chromatography of ions or potassium persulfate oxidation-ultraviolet
Spectrophotometry；The concentration of ammonia nitrogen is using salicylic acid-hypochlorite photometry measure；The concentration of nitrate nitrogen uses ion color
Spectrometry or determined by ultraviolet spectrophotometry；The concentration of nitrite nitrogen uses the chromatography of ions or N- (1- naphthyls)-ethylenediamine luminosity
Method determines.
10. determined according to the degradable soluble organic nitrogen of a kind of saprobia based on dynamic cultivation described in claim 9
Method, it is characterised in that：BDON calculation formula described in step (5) are：BDON=DONinf- DONeff。
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| CN113138170A (en) * | 2021-03-16 | 2021-07-20 | 同济大学 | Method for measuring biodegradable soluble organic nitrogen of drinking water |
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