CN107509739A - A kind of composite bactericide for preventing and treating bakanae disease of rice and its application - Google Patents
A kind of composite bactericide for preventing and treating bakanae disease of rice and its application Download PDFInfo
- Publication number
- CN107509739A CN107509739A CN201610428312.1A CN201610428312A CN107509739A CN 107509739 A CN107509739 A CN 107509739A CN 201610428312 A CN201610428312 A CN 201610428312A CN 107509739 A CN107509739 A CN 107509739A
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- CN
- China
- Prior art keywords
- rice
- bactericide
- bakanae disease
- prochloraz
- preventing
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
Abstract
The present invention relates to a kind of composite bactericide for preventing and treating bakanae disease of rice and its application, the composite bactericide active component include the formamide of azophenlyene 1 and Prochloraz, and the weight ratio of the formamide of azophenlyene 1 and Prochloraz is 20:1~1:80.Compared with prior art, the present invention has advantages below：1. two kinds of active ingredients are low toxicity, have fewer environmental impacts；2. reducing the use of chemical pesticide, residues of pesticides are reduced；3. two kinds of compositions are without obvious cross resistance；4. two kinds of active components compounds, there is obvious synergistic effect；5. delaying the generation of active ingredient resistance, the labour cost of peasant is reduced.
Description
Technical field
The present invention relates to a kind of bactericide, more particularly, to a kind of composite bactericide for preventing and treating bakanae disease of rice and its answers
With.
Background technology
Natural compound phenazine has the function that the suppression plant pathogenic fungi of wide spectrum, and azophenlyene -1- formamides are isolated from vacation
The microbe metabolite of unit cell, available for crop disease control, such as azophenlyene -1- formamides have significantly to sickle-like bacteria pathogen
Inhibitory action.
Prochloraz is low toxicity, efficient, wide-spectrum bactericide, has good permeability, there is protection and the double effects rooted out.
Pass through the biosynthesis for the important composition composition sterol for suppressing cell membrane, the metabolism of interference cell, so as to reach sterilization
Purpose.Prochloraz is conventional to use preventing and treating gourd, fruit and vegetable anthracnose, tikka to disease excellent caused by sac fungus and Fungi Imperfecti
Disease, it can also prevent and treat bakanae disease of rice, rice blast, citrus anthracnose, blossom-end rot, penicilliosis, green mould, banana anthracnose, tikka
The vegetables anthracnose such as disease, mango anthracnose, leaf spot of peanut, capsicum, eggplant, muskmelon, tomato, Strawberry anthracnose, rice bakanae disease
Disease, rice blast, sclerotinia sclerotiorum, leaf spot, brown spot, apple anthracnose, pear scab etc..
Bakanae disease of rice is also known as excessive growth disease, and each rice region of China has generation.Sick grain does not often germinate or can not be unearthed after broadcasting.
Seedling stage morbidity sick seedling is thinner and taller than strong seedling, and root system development is bad, and part sick seedling is dead before transplanting.Sick light ears ahead of time, fringe shape
It is small and unreal.Heading stage grain also can be aggrieved, serious browning, it is impossible to solid.Infected seed and invalid body are that this is pathogenetic
Primary source of infection, conidium pollution disease-free seed during seed soaking on infected seed and infect.After there is bakanae disease in field in rice
Substantially have no idea to prevent and treat, in the market medicament prevents and treats bakanae disease of rice with method for treating seeds, but Prochloraz is a large amount of long-term
Use, produced the resistance problems of pathogen.Therefore need to develop the new paragon for preventing and treating bakanae disease of rice.
At present chemical pesticide rapidly and efficiently the advantages of it by a large amount of long-term uses is result in crop disease, worm, grass anti-
The property of medicine occurs, a series of problems, such as also result in Environment Ecological Safe and food security.But a kind of novel germicide cost of exploitation compared with
Greatly, and the compounding cost of agricultural chemicals is relatively low, and can enhancing effect, delay the pathogen resistance to the action of a drug, moreover it is possible to expand antimicrobial spectrum.And biological agriculture
The characteristics of the nuisanceless of medicine, selectivity are by force and being compatible with the environment property is good makes it have stronger potentiality, and can reduce chemical pesticide makes
Dosage, promote the action of Pesticide use amount zero growth rate and diseases and pests of agronomic crop Sustainable Treatment, grain green volume increase.
Chinese patent CN100393211C discloses compound 2- cyano group -3- amino 3- ethyl phenylacrylates preventing and treating rice
The application of bakanae disease, and the compound and the synergetic pesticide composition of other bactericide, it is with 2- cyano group -3- amino -3- benzene
Base ethyl acrylate and bactericide are effective ingredient, and formulation is suspension seed treatment, missible oil, water dispersion granule, user
Method is seed treatment or foliar spray, can effectively prevent and treat the rice diseases such as bakanae disease of rice, improve Rice Production yield and
Quality.But the compound has the drawbacks of environmental pollution.
Chinese patent CN104365651A discloses a kind of Prochloraz microcapsule suspending agent and its complex preparation.The Prochloraz is micro-
The raw material of capsule suspending agent includes Prochloraz, solvent, emulsifying agent, wetting dispersing agent, cyst material, initiator and water.The invention solution
Prochloraz of having determined is unsuitable for making the technical problem of suspending agent, while microcapsule suspension-suspension seed-coating agent that the invention provides can have
The pest and disease damages such as effect preventing and treating bakanae disease of rice, black streaked dwarf virus of rice, thrips and planthopper, have good slow release effect.But
The still single biocidal efficacies of Prochloraz of the patent utilization.
The content of the invention
The problems such as it is an object of the invention to tackle the Environment Ecological Safe being on the rise and food security and reply disease
Opportunistic pathogen chemical pesticide resistance problems, there is provided a kind of composite bactericide with synergistic effect and safety and environmental protection, realize and improve medicine
Effect, the purpose for reducing pollution, reducing cost.
The purpose of the present invention can be achieved through the following technical solutions：
A kind of composite bactericide for preventing and treating bakanae disease of rice, its active component include azophenlyene -1- formamides and Prochloraz, fen
The weight of piperazine -1- formamides and Prochloraz ratio is 20:1~1:80.
Suspending agent, water dispersible granules, missible oil or aqueous emulsion is made by active component and auxiliary material in described composite bactericide.Match somebody with somebody
With auxiliary agent known to a person skilled in the art, various formulations are made in method known to a person skilled in the art, including it is outstanding
Floating agent, water dispersible granules, missible oil, aqueous emulsion.
In described composite bactericide, the percetage by weight of active component is 5-40%.
A kind of preferred implementation, when described composite bactericide is suspending agent, by weight percentage, its specifically into
Divide as follows：
A kind of preferred implementation, when described composite bactericide is water dispersible granules, by weight percentage, it has
Body composition is as follows：
A kind of preferred implementation, when described composite bactericide is aqueous emulsion, by weight percentage, its specifically into
Divide as follows：
Described composite bactericide is used to prevent and treat bakanae disease of rice.When specifically used, by described compound sterilizing dilution agent
Seed soaking after 1000-2000 times.
Compared with prior art, composite bactericide of the present invention has the characteristics that：
1st, two kinds of active ingredients are low toxicity, are had fewer environmental impacts；
2nd, reduce the use of chemical pesticide, reduce residues of pesticides；
3rd, two kinds of compositions are without obvious cross resistance；
4th, two kinds of active components compounds, and has obvious synergistic effect；
5th, delay the generation of active ingredient resistance, reduce the labour cost of peasant.
Brief description of the drawings
Fig. 1：Azophenlyene -1- formamides and Prochloraz compound proportion 1：5 pairs of bakanae disease of rice raw test photos.
Embodiment
The synergistic effect compounded for two kinds of active ingredients of checking azophenlyene -1- formamides and Prochloraz, first by both
Composition has carried out indoor biometricses experiment, and test method and result are as follows：
After the action character for analyzing two kinds of active ingredient first, the raw object surveyed is set to Fusarium moniliforme
(Fusarium moniliforme), the Pseudomonas Deuteromycotina fungi.Infected seed and invalid body are leaned in the propagation of the germ, can
To cause a variety of grass diseases, such as bakanae disease of rice, wheat scab.
The test method of indoor biometricses：The suppression grown using Activities measure medicament to bakanae disease of rice mycelia
Make and use.On the basis of trial test, from each medicament to designing 5 ladders in the range of germ mycelial growth inhibition rate 10%~90%
Concentration is spent, first dissolves 95% azophenlyene -1- formamides active compound and 95% Prochloraz active compound by solvent of dimethylformamide, is used
The decoction that the 0.1% Tween 80 aqueous solution is diluted to graded series concentration is standby, and then decoction is added in 1% ratio and has been melted
Change and be cooled in the PDA culture medium of room temperature and graded series concentration pastille culture medium is made, after fully shaking up, pour into the training of sterilizing
Support in ware (Φ 90mm) and drug containing flat board is made, four repetitions are often handled, to add the sterile water process of equivalent as blank control；With going out
Card punch (Φ 6mm) picking fusarium moniliforme bacterium dish of bacterium, the face-down aseptic inoculation of mycelia are placed in the center of drug containing flat board
Cultivated in 25 DEG C of constant temperature dark culturing case.Using crossing method measurement colony diameter when colony diameter reaches flat board 2/3.
Each medicament is calculated to concentration EC in the suppression of pathogen with least square method50, and poison altogether is calculated according to the following equation
Coefficient CTC, compare the virulence of different agents.Active compound used in above-mentioned raw test and reagent are product commercially available on the market.
The co-toxicity of mixture preparation is represented using Sun Yunpei co-toxicity coefficient method：
Mixture preparation theoretical toxicity index TTI=Σ (the toxicity index TI of medicament × its in mixture the medicine effectively into
The percentage divided).
It is synergistic effect during co-toxicity coefficient CTC >=120, is summation action when between 80 ＜ CTC ＜ 120, is during CTC≤80
Antagonism.
Azophenlyene -1- formamides and Prochloraz and different ratio are shown in Table 1 to the measurement result of bakanae disease of rice raw test, fen
Piperazine -1- formamides and Prochloraz compound proportion 1：5 pairs of rice bakanae disease diease occurrences survey photo and see Fig. 1.
The measurement result of the azophenlyene -1- formamides of table 1 and Prochloraz different ratio to bakanae disease of rice raw test
When azophenlyene -1- formamides and Prochloraz compound in varing proportions it can be seen from the data of table 1, the common poison system of formula
Number CTC is all higher than 120, there is obvious synergistic effect.Harnessed the river it can be seen that the compounding of azophenlyene -1- formamides and Prochloraz can turn into anti-
The favourable agents of the Bakanae disease of rice.
Testing clear and definite two kinds of active ingredient compoundings of azophenlyene -1- formamides and Prochloraz by indoor biometricses has increasing well
Effect acts on, below by way of specific embodiment to be further described the present invention, but the present invention be only limited to absolutely not it is following these
Embodiment.Percentage is mass percent in formula, and used active compound and auxiliary agent are the purchase of other producers.
Embodiment 1
6% azophenlyene -1- Jia Xian An ﹒ Prochloraz suspension emulsions
Embodiment 2
10% azophenlyene -1- Jia Xian An ﹒ Prochloraz suspending agents
Embodiment 3
11% azophenlyene -1- Jia Xian An ﹒ Prochloraz suspending agents
Embodiment 4
17% azophenlyene -1- formamide Prochloraz suspension emulsions
Embodiment 5
21% azophenlyene -1- Jia Xian An ﹒ Prochloraz suspension emulsions
Embodiment 6
25% azophenlyene -1- Jia Xian An ﹒ Prochloraz suspension emulsions
Embodiment 7
32% azophenlyene -1- Jia Xian An ﹒ Prochloraz suspension emulsions
Embodiment 8
24% azophenlyene -1- Jia Xian An ﹒ Prochloraz water dispersant grains
Embodiment 9
12% azophenlyene -1- Jia Xian An ﹒ prochloraz aqueous emulsions
Embodiment 10
Selection various embodiments above by seed-soaking method prevent and treat the experiment of bakanae disease of rice, it is determined that formula preventing and treating rice
The effect of bakanae disease, while by being the Contrast on effect compareed with 25% Prochloraz EC and clear water, verify the synergy effect after compounding
Fruit.
Test process：Medicament described in this experiment above example and above-mentioned control group dilute 2000 times of seed soakings.For
Rice varieties are tried as military fortune round-grained rice 30 and town rice 19, seed soaking time 48h, 25 DEG C of temperature, rear vernalization is to showing money or valuables one carries unintentionally.Seedling raising method is drought
Seedling, to transplanting crop field after the leaf phase at the length of time rice seedlings grow five.Seedling stage and phase pustulation period investigation bakanae disease strain rate simultaneously calculate preventive effect.The results are shown in Table 2,
Table 3.
Table 2, medicament of the embodiment of the present invention are to the prevention effect kind of bakanae disease of rice：Force fortune round-grained rice 30
Table 3, various embodiments of the present invention medicament are to the prevention effect kind of bakanae disease of rice：Town rice 19
Result of the test shows, after the above-mentioned Medicament soaked seed of the present invention, for the military fortune round-grained rice 30 of two kinds of rice varieties for examination
Number and town rice 19, prevention effect is apparently higher than 25% Prochloraz preventive effect.It can be seen that compound drug of the present invention has to bakanae disease of rice
Preferable preventive effect, the purpose for improving drug effect, reducing pollution, reducing cost is can reach, there is splendid economic value.
The above-mentioned description to embodiment is understood that for ease of those skilled in the art and using invention.
Person skilled in the art obviously can easily make various modifications to these embodiments, and described herein general
Principle is applied in other embodiment without by performing creative labour.Therefore, the invention is not restricted to above-described embodiment, ability
Field technique personnel do not depart from improvement that scope made and modification all should be the present invention's according to the announcement of the present invention
Within protection domain.
Claims (8)
1. it is a kind of prevent and treat bakanae disease of rice composite bactericide, it is characterised in that its active component include azophenlyene -1- formamides and
The weight ratio of Prochloraz, azophenlyene -1- formamides and Prochloraz is 20:1~1:80.
A kind of 2. composite bactericide for preventing and treating bakanae disease of rice according to claim 1, it is characterised in that described compounding
Suspending agent, water dispersible granules, missible oil or aqueous emulsion is made by active component and auxiliary material in bactericide.
A kind of 3. composite bactericide for preventing and treating bakanae disease of rice according to claim 2, it is characterised in that described compounding
In bactericide, the percetage by weight of active component is 5-40%.
4. a kind of composite bactericide for preventing and treating bakanae disease of rice according to claim 2, it is characterised in that when described is answered
When with bactericide being suspending agent, by weight percentage, its specific composition is as follows：
5. a kind of composite bactericide for preventing and treating bakanae disease of rice according to claim 2, it is characterised in that when described is answered
When with bactericide being water dispersible granules, by weight percentage, its specific composition is as follows：
6. a kind of composite bactericide for preventing and treating bakanae disease of rice according to claim 2, it is characterised in that when described is answered
When with bactericide being aqueous emulsion, by weight percentage, its specific composition is as follows：
7. the application of a kind of composite bactericide as any one of claim 1-6, it is characterised in that it is used for anti-harness the river
The Bakanae disease of rice.
8. the application of composite bactericide according to claim 7, it is characterised in that by described compound sterilizing dilution agent
Seed soaking after 1000-2000 times.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610428312.1A CN107509739A (en) | 2016-06-16 | 2016-06-16 | A kind of composite bactericide for preventing and treating bakanae disease of rice and its application |
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| CN201610428312.1A CN107509739A (en) | 2016-06-16 | 2016-06-16 | A kind of composite bactericide for preventing and treating bakanae disease of rice and its application |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155936A (en) * | 2011-12-18 | 2013-06-19 | 深圳诺普信农化股份有限公司 | Plant disease control composition containing phenazino-1-carboxylic acid |
| CN103642714A (en) * | 2013-11-13 | 2014-03-19 | 上海交通大学 | Gene engineering bacterial strain used for producing phenazine-1-methanamide and its purpose |
-
2016
- 2016-06-16 CN CN201610428312.1A patent/CN107509739A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155936A (en) * | 2011-12-18 | 2013-06-19 | 深圳诺普信农化股份有限公司 | Plant disease control composition containing phenazino-1-carboxylic acid |
| CN103642714A (en) * | 2013-11-13 | 2014-03-19 | 上海交通大学 | Gene engineering bacterial strain used for producing phenazine-1-methanamide and its purpose |
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Application publication date: 20171226 |